WMU05418 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU05418
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Delta(7)-sterol-C5(6)-desaturase
Location	Chr7 : 31331662 .. 31333485 (-)
Sequence length	960

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

ATTGTACTGCCTCCTTCCAACTTTTCTGAATACGTGGTTGAACATGATTGGACAAAATGCTATCCAAGGATACTCGATGTCGGGTGGCCTGCCTATGCCTTCCATATAATTACTTATATTGCCTTTCTAGAGTTTTCTATCTATTGGATGCATAGGGGGCTACATGATATAAAGCCTCTTTTACAAATATCTTCATGCAAAACACCATGTATACAACAAGAAAATTATTCTCTCTCCATTTGCTGGTTTAGCATTTCACCCTGTCGATGGGATATTGCAAGCAATACCTCATTTGTTTGCTCTATTTCTCATTCCAACCCATTTTCGAACACATATTTGTGCTGCTATTCTTCGAGGTGGTATGGACGGCAAACATTCACGATGGCATCCACGCACAAATGTGGCCAGTTCTGGGTGCTGGCTACCACACGATTCATCATACAAGATACCGCTACAATTATGGTCACTACTCCATATGGATGGATTGGATGTTTGGGACACTTCTTGATCCAATGGATACAGAAGCCAAAAGGGTTGTGATTGGTTGCTTCGACATGAATTGCTTACTCGTTATAGCTTTTGGTGATTCTTTGTGATCAATGTAAATCAAGTTAGTTATTGATAAAGCCCGGGCTTCTAGTATGTAACAGAATAAATTGCCATTCCAGGGTAATTTGCGAAGCCATTCATACCATCAAATCAAACATTTAATCTCAGAGTTCAGGAATCCACAGAGCCTGTGTGCTGCTGCTGCTGCTATTGGTAAGTAAAACTTGTGGACGCATTAGTTGTATCTTTGTATATCATATTTAAATGAAAACTTGTGTGAAAAAAGAAAAAAAGAAAAACAATGACCATGTTTGGGGAATGGACTTCATGATTTCCCCATCTTCAATTTGTGCAAGTTTTTCACATGTAATTGTATGAAATCGGAAAAGGAAAGCTAGTCATATTGCTACG

Library


Library category	ESTs
watermelon fruit 18d library	10
watermelon fruit 26d library	12
watermelon fruit 34d library	1

Homology

[Close]

BLAST of WMU05418 vs. TAIR10
Match: AT3G02580.1 (AT3G02580.1 sterol 1)

HSP 1 Score: 111.7 bits (278), Expect = 8.4e-25
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPM 401
           LLF E +WTANIHD IH  +WPV+GAGYHTIHHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 210 LLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYKHNYGHYTIWMDWMFGSLRDPL 267

Query: 402  461
           
Sbjct: 270  267

Query: 462  516
           
Sbjct: 330  267

HSP 2 Score: 95.1 bits (235), Expect = 8.2e-20
Identity = 40/52 (76.92%), Postives = 44/52 (84.62%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHI 241
           YKYLHA HH+YNK+  LSPFAGLAFHPVDGILQA+PH+ ALF++P HF THI
Sbjct: 157 YKYLHATHHIYNKQNTLSPFAGLAFHPVDGILQAVPHVIALFIVPIHFTTHI 208

Query: 242  301
           
Sbjct: 217  208

Query: 302  338
           
Sbjct: 277  208

HSP 3 Score: 70.5 bits (171), Expect = 2.2e-12
Identity = 29/52 (55.77%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 84
           SE ++E  WTKC+  I + GW  Y  +I  Y+ F+EF IYWMHR LHDIKPL
Sbjct: 105 SESMIERGWTKCFASIGEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPL 156

Query: 85   144
           
Sbjct: 165  156

Query: 145  181
           
Sbjct: 225  156

[Close]

BLAST of WMU05418 vs. TAIR10
Match: AT3G02590.1 (AT3G02590.1 Fatty acid hydroxylase superfamily protein)

HSP 1 Score: 106.3 bits (264), Expect = 3.5e-23
Identity = 40/58 (68.97%), Postives = 49/58 (84.48%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPM 401
           LLF E +WTA+IHD IH  +WP++GAGYHTIHHT Y++NYGHY+IWMDWMFG+L+ P+
Sbjct: 211 LLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYKHNYGHYTIWMDWMFGSLMVPL 268

Query: 402  461
           
Sbjct: 271  268

Query: 462  516
           
Sbjct: 331  268

HSP 2 Score: 90.5 bits (223), Expect = 2.0e-18
Identity = 39/59 (66.10%), Postives = 45/59 (76.27%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHICAAILRG 241
           YK+LHA HH+YNK+  LSPFAGLAFHP+DGILQAIPH+ ALF++P H  TH+    L G
Sbjct: 158 YKHLHATHHMYNKQNTLSPFAGLAFHPLDGILQAIPHVIALFIVPIHLITHLSLLFLEG 216

Query: 242  301
           
Sbjct: 218  216

Query: 302  359
           
Sbjct: 278  216

HSP 3 Score: 61.2 bits (147), Expect = 1.3e-09
Identity = 24/50 (48.00%), Postives = 30/50 (60.00%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIK 84
           SEY++EH WTKCY  +    W     +I  Y+  +EF IYW+H+ LHDIK
Sbjct: 106 SEYMIEHGWTKCYSTLDHFNWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155

Query: 85   144
           
Sbjct: 166  155

Query: 145  175
           
Sbjct: 226  155

[Close]

BLAST of WMU05418 vs. Swiss-Prot
Match: SC5D_TOBAC (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 6.7e-24
Identity = 45/71 (63.38%), Postives = 56/71 (78.87%), Query Frame = 3


Query: 324 FEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 383
           F     L+F E +WTANIHD IH +++PV+GAGYHTIHH  YR+NYGHY+IWMDWMFGTL
Sbjct: 201 FSTHIALIFLEALWTANIHDCIHGKVFPVMGAGYHTIHHRTYRHNYGHYTIWMDWMFGTL 260

Query: 384 LDPMDTEAKRV 443
            DP++ +AK++
Sbjct: 261 RDPVEEDAKKM 271

Query: 444  503
           
Sbjct: 321  271

Query: 504  537
           
Sbjct: 381  271

HSP 2 Score: 92.8 bits (229), Expect = 7.2e-18
Identity = 40/57 (70.18%), Postives = 44/57 (77.19%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHICAAIL 241
           YKYLHA HH+YNK+  LSPFAGLAFHP+DGILQA+PH+ AL L+P HF THI    L
Sbjct: 154 YKYLHATHHIYNKQNTLSPFAGLAFHPLDGILQAVPHVVALLLVPMHFSTHIALIFL 210

Query: 242  301
           
Sbjct: 214  210

Query: 302  353
           
Sbjct: 274  210

HSP 3 Score: 76.6 bits (187), Expect = 5.4e-13
Identity = 30/54 (55.56%), Postives = 37/54 (68.52%), Query Frame = 1


Query: 19  NFSEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 78
           + SEY++E+ WTKC+ RI DVGW +Y  +   Y+  +EF IYWMH  LHDIKPL
Sbjct: 100 SLSEYMIENGWTKCFARISDVGWLSYVIYAAIYLVIVEFGIYWMHMELHDIKPL 153

Query: 79   138
           
Sbjct: 160  153

Query: 139  181
           
Sbjct: 220  153

[Close]

BLAST of WMU05418 vs. Swiss-Prot
Match: SC5D1_ARATH (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-23
Identity = 43/58 (74.14%), Postives = 49/58 (84.48%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPM 401
           LLF E +WTANIHD IH  +WPV+GAGYHTIHHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 210 LLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYKHNYGHYTIWMDWMFGSLRDPL 267

Query: 402  461
           
Sbjct: 270  267

Query: 462  516
           
Sbjct: 330  267

HSP 2 Score: 95.1 bits (235), Expect = 1.5e-18
Identity = 40/52 (76.92%), Postives = 44/52 (84.62%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHI 241
           YKYLHA HH+YNK+  LSPFAGLAFHPVDGILQA+PH+ ALF++P HF THI
Sbjct: 157 YKYLHATHHIYNKQNTLSPFAGLAFHPVDGILQAVPHVIALFIVPIHFTTHI 208

Query: 242  301
           
Sbjct: 217  208

Query: 302  338
           
Sbjct: 277  208

HSP 3 Score: 70.5 bits (171), Expect = 3.8e-11
Identity = 29/52 (55.77%), Postives = 34/52 (65.38%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 84
           SE ++E  WTKC+  I + GW  Y  +I  Y+ F+EF IYWMHR LHDIKPL
Sbjct: 105 SESMIERGWTKCFASIGEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPL 156

Query: 85   144
           
Sbjct: 165  156

Query: 145  181
           
Sbjct: 225  156

[Close]

BLAST of WMU05418 vs. Swiss-Prot
Match: SC5D2_ARATH (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.3e-22
Identity = 40/58 (68.97%), Postives = 49/58 (84.48%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPM 401
           LLF E +WTA+IHD IH  +WP++GAGYHTIHHT Y++NYGHY+IWMDWMFG+L+ P+
Sbjct: 211 LLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYKHNYGHYTIWMDWMFGSLMVPL 268

Query: 402  461
           
Sbjct: 271  268

Query: 462  516
           
Sbjct: 331  268

HSP 2 Score: 90.5 bits (223), Expect = 3.6e-17
Identity = 39/59 (66.10%), Postives = 45/59 (76.27%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHICAAILRG 241
           YK+LHA HH+YNK+  LSPFAGLAFHP+DGILQAIPH+ ALF++P H  TH+    L G
Sbjct: 158 YKHLHATHHMYNKQNTLSPFAGLAFHPLDGILQAIPHVIALFIVPIHLITHLSLLFLEG 216

Query: 242  301
           
Sbjct: 218  216

Query: 302  359
           
Sbjct: 278  216

HSP 3 Score: 61.2 bits (147), Expect = 2.3e-08
Identity = 24/50 (48.00%), Postives = 30/50 (60.00%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIK 84
           SEY++EH WTKCY  +    W     +I  Y+  +EF IYW+H+ LHDIK
Sbjct: 106 SEYMIEHGWTKCYSTLDHFNWFLCFLYIALYLVLVEFMIYWVHKELHDIK 155

Query: 85   144
           
Sbjct: 166  155

Query: 145  175
           
Sbjct: 226  155

[Close]

BLAST of WMU05418 vs. TrEMBL
Match: A0A0A0L3B9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.8e-31
Identity = 61/69 (88.41%), Postives = 64/69 (92.75%), Query Frame = 3


Query: 324 FEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 383
           F    VLLFFEVVWTANIHDGIH++MWPV+GAGYHTIHHTRYRYNYGHYSIWMDWMFGTL
Sbjct: 204 FRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 263

Query: 384 LDPMDTEAK 443
           LDPMD EAK
Sbjct: 264 LDPMDIEAK 272

Query: 444  503
           
Sbjct: 324  272

Query: 504  531
           
Sbjct: 384  272

[Close]

BLAST of WMU05418 vs. TrEMBL
Match: A0A0A0L3B9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.0e-23
Identity = 51/66 (77.27%), Postives = 56/66 (84.85%), Query Frame = 1


Query: 4   VLPPSNFSEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPLL 63
           VLP   F+EYVVEHDWTKCYPR+LDVGWPAYAFHIITY+ F+EF IYWMHRGLHDIKPL 
Sbjct: 100 VLP--TFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLY 159

Query: 64  QISSCK 123
           +    K
Sbjct: 160 KYLHAK 163

Query: 124  183
           
Sbjct: 220  163

Query: 184  202
           
Sbjct: 280  163

HSP 2 Score: 113.6 bits (283), Expect = 4.4e-22
Identity = 59/93 (63.44%), Postives = 64/93 (68.82%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHICAAILR-- 241
           YKYLHAKHH+YNKKI LSPFAGLAFHP+DGILQAIPHLFALFLIPTHFRTHI        
Sbjct: 157 YKYLHAKHHIYNKKITLSPFAGLAFHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVV 216

Query: 242 ---GGMDGKHSRWHPRTNVASSGCWLPHDSSYK 301
                 DG HSR  P   V  +G    H + Y+
Sbjct: 217 WTANIHDGIHSRMWP---VMGAGYHTIHHTRYR 246

Query: 302  361
           
Sbjct: 277  246

Query: 362  421
           
Sbjct: 337  246

Query: 422  446
           
Sbjct: 397  246

HSP 3 Score: 122.9 bits (307), Expect = 7.2e-25
Identity = 51/80 (63.75%), Postives = 61/80 (76.25%), Query Frame = 3


Query: 297 LLYFSFQPIFEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSI 356
           L  F     F     LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY+I
Sbjct: 192 LALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTI 251

Query: 357 WMDWMFGTLLDPMDTEAKRV 416
           WMDWMFGTL DP++ EAK++
Sbjct: 252 WMDWMFGTLRDPVEDEAKKI 271

Query: 417  476
           
Sbjct: 312  271

Query: 477  536
           
Sbjct: 372  271

[Close]

BLAST of WMU05418 vs. TrEMBL
Match: A0A022RY23_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a011765mg PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.9e-17
Identity = 42/52 (80.77%), Postives = 47/52 (90.38%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHI 241
           YKYLHA HH+YNK+  LSPFAGLAFHP+DGILQA+PH+ ALFLIPTHF THI
Sbjct: 154 YKYLHATHHIYNKQNTLSPFAGLAFHPIDGILQAVPHVLALFLIPTHFTTHI 205

Query: 242  301
           
Sbjct: 214  205

Query: 302  338
           
Sbjct: 274  205

HSP 2 Score: 84.7 bits (208), Expect = 2.2e-13
Identity = 34/52 (65.38%), Postives = 41/52 (78.85%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 84
           SEY++EH WTKC+ RI DVGW AY  ++  Y+ F+EF IYWMHR LHDIKPL
Sbjct: 102 SEYMIEHGWTKCFSRISDVGWIAYFCYLAVYLVFVEFGIYWMHRELHDIKPL 153

Query: 85   144
           
Sbjct: 162  153

Query: 145  181
           
Sbjct: 222  153

HSP 3 Score: 121.7 bits (304), Expect = 1.6e-24
Identity = 47/65 (72.31%), Postives = 57/65 (87.69%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDT 401
           LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY++WMDWMFGTL DP++ 
Sbjct: 208 LLFIEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTVWMDWMFGTLRDPLED 267

Query: 402 EAKRV 461
           EAK +
Sbjct: 268 EAKNI 272

Query: 462  521
           
Sbjct: 328  272

Query: 522  537
           
Sbjct: 388  272

[Close]

BLAST of WMU05418 vs. TrEMBL
Match: A0A118K5B7_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_012760 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.2e-17
Identity = 41/52 (78.85%), Postives = 47/52 (90.38%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHI 241
           YKYLHA HH+YNK+  LSPFAGLAFHP+DGILQA+PH+ ALFL+PTHF THI
Sbjct: 155 YKYLHATHHIYNKQNTLSPFAGLAFHPLDGILQAVPHVIALFLVPTHFTTHI 206

Query: 242  301
           
Sbjct: 215  206

Query: 302  338
           
Sbjct: 275  206

HSP 2 Score: 78.2 bits (191), Expect = 2.0e-11
Identity = 33/59 (55.93%), Postives = 43/59 (72.88%), Query Frame = 1


Query: 4   VLPPSNFSEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 63
           VLP    SEY+VE+ WT+C+ RI D+GW +Y +++  Y   +EF IYWMHR LHDIKPL
Sbjct: 98  VLP--TISEYMVENGWTRCFSRISDIGWASYIWYLGLYFVIVEFGIYWMHRELHDIKPL 154

Query: 64   123
           
Sbjct: 158  154

Query: 124  181
           
Sbjct: 218  154

HSP 3 Score: 121.3 bits (303), Expect = 2.1e-24
Identity = 47/65 (72.31%), Postives = 58/65 (89.23%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDT 401
           LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY+IWMDWMFGTL DP++ 
Sbjct: 207 LLFMEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTIWMDWMFGTLRDPIED 266

Query: 402 EAKRV 461
           +AK++
Sbjct: 267 DAKKI 271

Query: 462  521
           
Sbjct: 327  271

Query: 522  537
           
Sbjct: 387  271

[Close]

BLAST of WMU05418 vs. TrEMBL
Match: A0A164UFV8_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025906 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.6e-16
Identity = 39/52 (75.00%), Postives = 46/52 (88.46%), Query Frame = 2


Query: 182 YKYLHAKHHVYNKKIILSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHI 241
           YKYLHA HH+YNK+  LSPFAGLAFHP+DGILQA+PH+ ALFL+P HF TH+
Sbjct: 154 YKYLHATHHIYNKQNTLSPFAGLAFHPLDGILQALPHVIALFLVPMHFTTHV 205

Query: 242  301
           
Sbjct: 214  205

Query: 302  338
           
Sbjct: 274  205

HSP 2 Score: 79.3 bits (194), Expect = 9.2e-12
Identity = 31/52 (59.62%), Postives = 41/52 (78.85%), Query Frame = 1


Query: 25  SEYVVEHDWTKCYPRILDVGWPAYAFHIITYIAFLEFSIYWMHRGLHDIKPL 84
           SEY+VE+ WT+C+P I  +GW  Y +++I Y+ F+EF IYWMHR LHDIKPL
Sbjct: 102 SEYMVENGWTRCFPSISSIGWLDYIWNLIGYLVFVEFGIYWMHRELHDIKPL 153

Query: 85   144
           
Sbjct: 162  153

Query: 145  181
           
Sbjct: 222  153

HSP 3 Score: 121.3 bits (303), Expect = 2.1e-24
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDT 401
           LLF E +WTANIHD IHA++WPV+GAGYHTIHHT YR+NYGHY+IWMDWMFGTL DP++ 
Sbjct: 207 LLFIEAIWTANIHDCIHAKVWPVMGAGYHTIHHTTYRHNYGHYTIWMDWMFGTLRDPVED 266

Query: 402 EAKRV 461
           E K++
Sbjct: 267 EVKKM 271

Query: 462  521
           
Sbjct: 327  271

Query: 522  537
           
Sbjct: 387  271

[Close]

BLAST of WMU05418 vs. NCBI nr
Match: gi|449465599|ref|XP_004150515.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus])

HSP 1 Score: 140.6 bits (353), Expect = 4.8e-30
Identity = 61/69 (88.41%), Postives = 64/69 (92.75%), Query Frame = 3


Query: 324 FEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 383
           F    VLLFFEVVWTANIHDGIH++MWPV+GAGYHTIHHTRYRYNYGHYSIWMDWMFGTL
Sbjct: 204 FRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 263

Query: 384 LDPMDTEAK 443
           LDPMD EAK
Sbjct: 264 LDPMDIEAK 272

Query: 444  503
           
Sbjct: 324  272

Query: 504  531
           
Sbjct: 384  272

[Close]

BLAST of WMU05418 vs. NCBI nr
Match: gi|659086753|ref|XP_008444093.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo])

HSP 1 Score: 140.6 bits (353), Expect = 4.8e-30
Identity = 61/69 (88.41%), Postives = 64/69 (92.75%), Query Frame = 3


Query: 324 FEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 383
           F    VLLFFEVVWTANIHDGIH++MWPV+GAGYHTIHHTRYRYNYGHYSIWMDWMFGTL
Sbjct: 204 FRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 263

Query: 384 LDPMDTEAK 443
           LDPMD EAK
Sbjct: 264 LDPMDIEAK 272

Query: 444  503
           
Sbjct: 324  272

Query: 504  531
           
Sbjct: 384  272

[Close]

BLAST of WMU05418 vs. NCBI nr
Match: gi|747102741|ref|XP_011099542.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase [Sesamum indicum])

HSP 1 Score: 121.3 bits (303), Expect = 3.0e-24
Identity = 49/71 (69.01%), Postives = 59/71 (83.10%), Query Frame = 3


Query: 324 FEHIFVLLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTL 383
           F     LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY+IWMDWMFGTL
Sbjct: 201 FSTHIALLFIEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTIWMDWMFGTL 260

Query: 384 LDPMDTEAKRV 443
            DP++ EAK++
Sbjct: 261 RDPVEDEAKKI 271

Query: 444  503
           
Sbjct: 321  271

Query: 504  537
           
Sbjct: 381  271

[Close]

BLAST of WMU05418 vs. NCBI nr
Match: gi|976924940|gb|KVI08863.1| (hypothetical protein Ccrd_012760 [Cynara cardunculus var. scolymus])

HSP 1 Score: 120.9 bits (302), Expect = 3.9e-24
Identity = 47/65 (72.31%), Postives = 57/65 (87.69%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDT 401
           LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY++WMDWMFGTL DP++ 
Sbjct: 208 LLFIEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTVWMDWMFGTLRDPLED 267

Query: 402 EAKRV 461
           EAK +
Sbjct: 268 EAKNI 272

Query: 462  521
           
Sbjct: 328  272

Query: 522  537
           
Sbjct: 388  272

[Close]

BLAST of WMU05418 vs. NCBI nr
Match: gi|1021031046|gb|KZM88831.1| (hypothetical protein DCAR_025906 [Daucus carota subsp. sativus])

HSP 1 Score: 120.6 bits (301), Expect = 5.1e-24
Identity = 47/65 (72.31%), Postives = 58/65 (89.23%), Query Frame = 3


Query: 342 LLFFEVVWTANIHDGIHAQMWPVLGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDT 401
           LLF E +WTANIHD IH ++WPV+GAGYHTIHHT YR+NYGHY+IWMDWMFGTL DP++ 
Sbjct: 207 LLFMEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNYGHYTIWMDWMFGTLRDPIED 266

Query: 402 EAKRV 461
           +AK++
Sbjct: 267 DAKKI 271

Query: 462  521
           
Sbjct: 327  271

Query: 522  537
           
Sbjct: 387  271

The following BLAST results are available for this feature:

BLAST of WMU05418 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G02580.1	8.4e-25	74.14	sterol 1	[more]
AT3G02590.1	3.5e-23	68.97	Fatty acid hydroxylase superfamily protein	[more]

BLAST of WMU05418 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SC5D_TOBAC	6.7e-24	63.38	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1	[more]
SC5D1_ARATH	1.5e-23	74.14	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2	[more]
SC5D2_ARATH	6.3e-22	68.97	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3...	[more]

BLAST of WMU05418 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3B9_CUCSA	8.8e-31	88.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1	[more]
A0A0A0L3B9_CUCSA	4.0e-23	77.27	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1	[more]
A0A022RY23_ERYGU	1.9e-17	80.77	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a011765mg PE=3 SV=1	[more]
A0A118K5B7_CYNCS	4.2e-17	78.85	Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_012760 PE=3 ...	[more]
A0A164UFV8_DAUCA	3.6e-16	75.00	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025906 PE=4 SV=1	[more]

BLAST of WMU05418 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449465599\|ref\|XP_004150515.1\|	4.8e-30	88.41	PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus]	[more]
gi\|659086753\|ref\|XP_008444093.1\|	4.8e-30	88.41	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo]	[more]
gi\|747102741\|ref\|XP_011099542.1\|	3.0e-24	69.01	PREDICTED: delta(7)-sterol-C5(6)-desaturase [Sesamum indicum]	[more]
gi\|976924940\|gb\|KVI08863.1\|	3.9e-24	72.31	hypothetical protein Ccrd_012760 [Cynara cardunculus var. scolymus]	[more]
gi\|1021031046\|gb\|KZM88831.1\|	5.1e-24	72.31	hypothetical protein DCAR_025906 [Daucus carota subsp. sativus]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR006694	Fatty_acid_hydroxylase

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006633	fatty acid biosynthetic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla010940	Cla010940	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S2_0001095	S2_0001095	EST
S2_0025948	S2_0025948	EST
S2_0050279	S2_0050279	EST
S2_0069463	S2_0069463	EST
S2_0094572	S2_0094572	EST
S2_0120200	S2_0120200	EST
S2_0137525	S2_0137525	EST
S2_0158293	S2_0158293	EST
S2_0164112	S2_0164112	EST
S2_0179029	S2_0179029	EST
S3_0032950	S3_0032950	EST
S3_0034415	S3_0034415	EST
S3_0043801	S3_0043801	EST
S3_0047780	S3_0047780	EST
S3_0059478	S3_0059478	EST
S3_0080757	S3_0080757	EST
S3_0109509	S3_0109509	EST
S3_0116056	S3_0116056	EST
S3_0124537	S3_0124537	EST
S3_0127299	S3_0127299	EST
S3_0163211	S3_0163211	EST
S3_0173767	S3_0173767	EST
S4_0120268	S4_0120268	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 7..78 score: 3.
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 7..89 score: 2.1
None	No IPR available	PANTHER	PTHR11863:SF33	LATHOSTEROL OXIDASE	coord: 7..89 score: 2.1