MU66156 (transcribed_cluster) Melon (DHL92) v3.5.1

Overview
NameMU66156
Typetranscribed_cluster
OrganismCucumis melo (Melon (DHL92) v3.5.1)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
Locationchr1 : 23384912 .. 23386345 (-)
Sequence length867
Genome Viewer
Sequences
The following sequences are available for this feature:

transcribed_cluster sequence

CTACGAAGCATGCTCTCAGGCTTCAACCATTTGTTCGTGATTTAGTTGGAGTAACGTTGGTGAGCAATCGATCAATGGCGCTATTCAAGCTTTTAACTATGGCCGCATCTCGAACAAGAAAACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACAGCGGCCCCACCTCCCAAACTCCTAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTACGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTACCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTTGCTCGTGGGGCTAGGAGACCTCTTTTGATTGGTGATTTGCCGTTTGGTACTTATGAATCCAGCTCTGCGCAGGATGTCAAAACCTTAAACAAATCAGTAACTTCTATTGGAGAGGTCGAGCCTGAGGCTAAAGGCATTGTGGCATGTGGGTGTAAACTGCATTCATGGAAGGGCTAGAGAAAATCTCAGTAGAGGATCGACTGATATTTGAGTTTTAGGCATATTATGGAATGGAAACACAGTTTCATGGAAGACCACGTCTCTTGGGATGAAGAACTTTTTGTGCTCAATGTCATACAGCTTGTAAAGCCTTCATGCCATGGGGATAGCCGATGAAGACAGAGGGAACAACTCTGGGATGAACTTGGAGCGATTGTGAGACAGTGAGGATGCATAGCATAAAGAGCCAAAAATTCTTTAAGAGATATAGTCAGAACATGGTTCTCTG
Library
Library categoryESTs
melon CM-TEa library1
Homology
[Close]
BLAST of MU66156 vs. Swiss-Prot
Match: PANB1_ARATH (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 7.3e-54
Identity = 104/138 (75.36%), Postives = 118/138 (85.51%), Query Frame = 3 

Query: 99  MAASRTRKQSLLK---TLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMV 158
           MA+S TR  S      ++R+MSN+PENTVY GP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 159 TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 218
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 219 GDLPFGTYESSSAQDVKT 278
           GDLPFGTYESSS+Q V T
Sbjct: 121 GDLPFGTYESSSSQAVDT 138

Query: 279  338
           
Sbjct: 181  138

Query: 339  398
           
Sbjct: 241  138

Query: 399  458
           
Sbjct: 301  138

Query: 459  504
           
Sbjct: 361  138

[Close]
BLAST of MU66156 vs. Swiss-Prot
Match: PANB2_ARATH (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.0e-52
Identity = 98/130 (75.38%), Postives = 111/130 (85.38%), Query Frame = 3 

Query: 114 TRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 173
           +R    + T+R+MSNVPE+TVY GP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 174 VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 233
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 234 ESSSAQDVKT 293
           ESS+ Q V T
Sbjct: 131 ESSTNQAVDT 140

Query: 294  353
           
Sbjct: 191  140

Query: 354  413
           
Sbjct: 251  140

Query: 414  473
           
Sbjct: 311  140

Query: 474  504
           
Sbjct: 371  140

[Close]
BLAST of MU66156 vs. Swiss-Prot
Match: PANB1_ORYSJ (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.2e-40
Identity = 85/130 (65.38%), Postives = 97/130 (74.62%), Query Frame = 3 

Query: 144 RYMSNVPENTVYSGPTSQT------------PNQRVTLTNLRQKYKKGEPITMVTAYDYP 203
           R +S+VPE+ VY GP  Q               +RVT+T LR K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 204 SGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFG 263
           S VH+D AGID+ LVGDSAAMVVHGHDTTLPISLD +L HCRAVARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 264 TYESSSAQDV 323
            YESSS + V
Sbjct: 138 CYESSSTRAV 147

Query: 324  383
           
Sbjct: 198  147

Query: 384  443
           
Sbjct: 258  147

Query: 444  498
           
Sbjct: 318  147

[Close]
BLAST of MU66156 vs. Swiss-Prot
Match: PANB2_ORYSJ (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.3e-36
Identity = 79/125 (63.20%), Postives = 90/125 (72.00%), Query Frame = 3 

Query: 144 RYMSN-VPENTVYSG------PTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHL 203
           R MSN   E  +Y G        S    +RVTL  LR K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 204 DMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESS 263
           D AG DI LVGDSAAMV HGHD TLPISLD ++ HCRAVARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 264 SAQDV 323
           +AQ V
Sbjct: 174 TAQAV 178

Query: 324  383
           
Sbjct: 234  178

Query: 384  443
           
Sbjct: 294  178

Query: 444  498
           
Sbjct: 354  178

[Close]
BLAST of MU66156 vs. Swiss-Prot
Match: PANB_EMENI (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN=panB PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.1e-23
Identity = 53/107 (49.53%), Postives = 70/107 (65.42%), Query Frame = 3 

Query: 183 GPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHG 242
           G     P ++VT+  LR  YKKGEPITM+TA+D+PS    D AG+D+ LVGDS AMV  G
Sbjct: 55  GAAQANPRKKVTMQTLRNLYKKGEPITMLTAHDFPSAHVADAAGMDMILVGDSLAMVALG 114

Query: 243 HDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESSSAQDVKT 302
              T  ++LD++LVHCR+VAR A+    + DLP G+YE S  Q +++
Sbjct: 115 MQDTSEVTLDDMLVHCRSVARAAQSAFTVSDLPMGSYEVSPEQALQS 161

Query: 303  362
           
Sbjct: 175  161

Query: 363  422
           
Sbjct: 235  161

Query: 423  482
           
Sbjct: 295  161

Query: 483  504
           
Sbjct: 355  161

[Close]
BLAST of MU66156 vs. TrEMBL
Match: A0A0A0K8B2_CUCSA (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.7e-70
Identity = 136/143 (95.10%), Postives = 138/143 (96.50%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 195 RPLLIGDLPFGTYESSSAQDVKT 254
           RPLLIGDLPFGTYESSSAQ + T
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDT 143

Query: 255  314
           
Sbjct: 181  143

Query: 315  374
           
Sbjct: 241  143

Query: 375  434
           
Sbjct: 301  143

Query: 435  494
           
Sbjct: 361  143

Query: 495  504
           
Sbjct: 421  143

[Close]
BLAST of MU66156 vs. TrEMBL
Match: B9I8Q3_POPTR (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Populus trichocarpa GN=POPTR_0014s08620g PE=3 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 6.7e-54
Identity = 106/143 (74.13%), Postives = 123/143 (86.01%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           M L   LT ++S  +  +LL+ LR+MSNVPENTVY GP  Q P+QRVTLT+L+QK+KKG+
Sbjct: 1   MGLLTYLTKSSSTLKPTTLLRHLRFMSNVPENTVYGGPKPQNPHQRVTLTHLKQKHKKGD 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PIT+VTAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+
Sbjct: 61  PITVVTAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAK 120

Query: 195 RPLLIGDLPFGTYESSSAQDVKT 254
           RPLL+GDLPFGTYESSS Q V T
Sbjct: 121 RPLLVGDLPFGTYESSSNQAVDT 143

Query: 255  314
           
Sbjct: 181  143

Query: 315  374
           
Sbjct: 241  143

Query: 375  434
           
Sbjct: 301  143

Query: 435  494
           
Sbjct: 361  143

Query: 495  504
           
Sbjct: 421  143

[Close]
BLAST of MU66156 vs. TrEMBL
Match: A0A067KNV3_JATCU (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Jatropha curcas GN=JCGZ_07886 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 5.7e-53
Identity = 108/143 (75.52%), Postives = 120/143 (83.92%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           MALF  L  ++S  +   L   LR MSNVPENTVY GP SQ PN+RVTLT L+QK+KKGE
Sbjct: 1   MALFAKLYRSSSVIKAHYLHHCLRSMSNVPENTVYGGPKSQNPNERVTLTQLKQKHKKGE 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PIT+VTAYDYPS VHLD AGIDI LVGDSAAMVVHGHDTTLPISL+E+LVHCRAVARGA+
Sbjct: 61  PITVVTAYDYPSAVHLDQAGIDICLVGDSAAMVVHGHDTTLPISLEEMLVHCRAVARGAK 120

Query: 195 RPLLIGDLPFGTYESSSAQDVKT 254
           RPLL+GDLPFGTYESS+ Q V T
Sbjct: 121 RPLLVGDLPFGTYESSTKQAVDT 143

Query: 255  314
           
Sbjct: 181  143

Query: 315  374
           
Sbjct: 241  143

Query: 375  434
           
Sbjct: 301  143

Query: 435  494
           
Sbjct: 361  143

Query: 495  504
           
Sbjct: 421  143

[Close]
BLAST of MU66156 vs. TrEMBL
Match: D7LSB0_ARALL (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_486638 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-52
Identity = 103/136 (75.74%), Postives = 118/136 (86.76%), Query Frame = 3 

Query: 96  TMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTA 155
           ++  S +R    + T+R+MSNVPENTVY GP SQ PNQRVTLT LRQK++KGEPITMVTA
Sbjct: 4   SLVRSCSRASRAITTVRFMSNVPENTVYGGPKSQNPNQRVTLTQLRQKHRKGEPITMVTA 63

Query: 156 YDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGD 215
           YDYPS VH+D AGID+ LVGDSAAMVVHG+DTTLPISLDE+LVHCRAVARGA+RPLL+GD
Sbjct: 64  YDYPSAVHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAKRPLLVGD 123

Query: 216 LPFGTYESSSAQDVKT 275
           LPFGTYESS+ Q V T
Sbjct: 124 LPFGTYESSTNQAVDT 139

Query: 276  335
           
Sbjct: 184  139

Query: 336  395
           
Sbjct: 244  139

Query: 396  455
           
Sbjct: 304  139

Query: 456  504
           
Sbjct: 364  139

[Close]
BLAST of MU66156 vs. TrEMBL
Match: A0A087H5I9_ARAAL (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Arabis alpina GN=AALP_AA4G251200 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.7e-52
Identity = 105/138 (76.09%), Postives = 120/138 (86.96%), Query Frame = 3 

Query: 99  MAASRTRKQSLLK---TLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMV 158
           MA+S TR  S      +LR+MSN+PENTVY GP  Q PNQRVTLT+LRQK+KKGEPIT+V
Sbjct: 1   MASSLTRNCSRFSKALSLRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHKKGEPITVV 60

Query: 159 TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 218
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAKRPLLV 120

Query: 219 GDLPFGTYESSSAQDVKT 278
           GDLPFGTYESS++Q V T
Sbjct: 121 GDLPFGTYESSTSQAVDT 138

Query: 279  338
           
Sbjct: 181  138

Query: 339  398
           
Sbjct: 241  138

Query: 399  458
           
Sbjct: 301  138

Query: 459  504
           
Sbjct: 361  138

[Close]
BLAST of MU66156 vs. TAIR10
Match: AT2G46110.1 (AT2G46110.1 ketopantoate hydroxymethyltransferase 1)

HSP 1 Score: 212.2 bits (539), Expect = 4.1e-55
Identity = 104/138 (75.36%), Postives = 118/138 (85.51%), Query Frame = 3 

Query: 99  MAASRTRKQSLLK---TLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMV 158
           MA+S TR  S      ++R+MSN+PENTVY GP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 159 TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 218
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 219 GDLPFGTYESSSAQDVKT 278
           GDLPFGTYESSS+Q V T
Sbjct: 121 GDLPFGTYESSSSQAVDT 138

Query: 279  338
           
Sbjct: 181  138

Query: 339  398
           
Sbjct: 241  138

Query: 399  458
           
Sbjct: 301  138

Query: 459  504
           
Sbjct: 361  138

[Close]
BLAST of MU66156 vs. TAIR10
Match: AT3G61530.1 (AT3G61530.1 Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 205.3 bits (521), Expect = 5.1e-53
Identity = 98/130 (75.38%), Postives = 111/130 (85.38%), Query Frame = 3 

Query: 114 TRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 173
           +R    + T+R+MSNVPE+TVY GP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 174 VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 233
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 234 ESSSAQDVKT 293
           ESS+ Q V T
Sbjct: 131 ESSTNQAVDT 140

Query: 294  353
           
Sbjct: 191  140

Query: 354  413
           
Sbjct: 251  140

Query: 414  473
           
Sbjct: 311  140

Query: 474  504
           
Sbjct: 371  140

[Close]
BLAST of MU66156 vs. NCBI nr
Match: gi|659092903|ref|XP_008447278.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo])

HSP 1 Score: 275.4 bits (703), Expect = 1.1e-70
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 195 RPLLIGDLPFGTYESSSAQDVKT 254
           RPLLIGDLPFGTYESSSAQ V T
Sbjct: 121 RPLLIGDLPFGTYESSSAQAVDT 143

Query: 255  314
           
Sbjct: 181  143

Query: 315  374
           
Sbjct: 241  143

Query: 375  434
           
Sbjct: 301  143

Query: 435  494
           
Sbjct: 361  143

Query: 495  504
           
Sbjct: 421  143

[Close]
BLAST of MU66156 vs. NCBI nr
Match: gi|449443772|ref|XP_004139651.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-68
Identity = 136/143 (95.10%), Postives = 138/143 (96.50%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 195 RPLLIGDLPFGTYESSSAQDVKT 254
           RPLLIGDLPFGTYESSSAQ + T
Sbjct: 121 RPLLIGDLPFGTYESSSAQAIDT 143

Query: 255  314
           
Sbjct: 181  143

Query: 315  374
           
Sbjct: 241  143

Query: 375  434
           
Sbjct: 301  143

Query: 435  494
           
Sbjct: 361  143

Query: 495  504
           
Sbjct: 421  143

[Close]
BLAST of MU66156 vs. NCBI nr
Match: gi|659098169|ref|XP_008450010.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo])

HSP 1 Score: 247.3 bits (630), Expect = 3.3e-62
Identity = 126/140 (90.00%), Postives = 131/140 (93.57%), Query Frame = 3 

Query: 75  MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 134
           MA+FKLLT  AS+TR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNL QKYKKGE
Sbjct: 1   MAVFKLLTWIASQTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLIQKYKKGE 60

Query: 135 PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 194
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGH++TLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHNSTLPISLDEMLVHCRAVARGAR 120

Query: 195 RPLLIGDLPFGTYESSSAQD 254
           RP LI DLPFGTYESS A D
Sbjct: 121 RPFLIVDLPFGTYESSHAVD 140

Query: 255  314
           
Sbjct: 181  140

Query: 315  374
           
Sbjct: 241  140

Query: 375  434
           
Sbjct: 301  140

Query: 435  494
           
Sbjct: 361  140

[Close]
BLAST of MU66156 vs. NCBI nr
Match: gi|698473793|ref|XP_009784538.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial-like [Nicotiana sylvestris])

HSP 1 Score: 218.4 bits (555), Expect = 1.6e-53
Identity = 106/131 (80.92%), Postives = 116/131 (88.55%), Query Frame = 3 

Query: 111 RTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPS 170
           R   Q+  + LRYMSNVPENTVY GP  Q PNQRVTLTNLRQK+KKGEPITMVT YDYPS
Sbjct: 16  RKNVQTHYRKLRYMSNVPENTVYGGPKPQNPNQRVTLTNLRQKHKKGEPITMVTGYDYPS 75

Query: 171 GVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGT 230
           GVH+DMAGIDI LVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGA+RPLL+GDLPFGT
Sbjct: 76  GVHIDMAGIDICLVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAKRPLLVGDLPFGT 135

Query: 231 YESSSAQDVKT 290
           YESS+ Q V T
Sbjct: 136 YESSTKQAVDT 146

Query: 291  350
           
Sbjct: 196  146

Query: 351  410
           
Sbjct: 256  146

Query: 411  470
           
Sbjct: 316  146

Query: 471  504
           
Sbjct: 376  146

[Close]
BLAST of MU66156 vs. NCBI nr
Match: gi|697122926|ref|XP_009615453.1| (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial-like [Nicotiana tomentosiformis])

HSP 1 Score: 216.5 bits (550), Expect = 6.2e-53
Identity = 105/131 (80.15%), Postives = 116/131 (88.55%), Query Frame = 3 

Query: 111 RTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPS 170
           R   Q+  + LRYMSNVPENTVY GP  Q PNQRVTLT+LRQK+KKGEPITMVT YDYPS
Sbjct: 16  RKNVQTHYRKLRYMSNVPENTVYGGPKPQNPNQRVTLTSLRQKHKKGEPITMVTGYDYPS 75

Query: 171 GVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGT 230
           GVH+DMAGIDI LVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGA+RPLL+GDLPFGT
Sbjct: 76  GVHIDMAGIDICLVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAKRPLLVGDLPFGT 135

Query: 231 YESSSAQDVKT 290
           YESS+ Q V T
Sbjct: 136 YESSTKQAVDT 146

Query: 291  350
           
Sbjct: 196  146

Query: 351  410
           
Sbjct: 256  146

Query: 411  470
           
Sbjct: 316  146

Query: 471  504
           
Sbjct: 376  146

The following BLAST results are available for this feature:
BLAST of MU66156 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PANB1_ARATH7.3e-5475.363-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
PANB2_ARATH9.0e-5275.383-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
PANB1_ORYSJ1.2e-4065.383-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
PANB2_ORYSJ5.3e-3663.203-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
PANB_EMENI5.1e-2349.533-methyl-2-oxobutanoate hydroxymethyltransferase OS=Emericella nidulans (strain ... [more]
BLAST of MU66156 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K8B2_CUCSA8.7e-7095.103-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus GN=Csa_7G338... [more]
B9I8Q3_POPTR6.7e-5474.133-methyl-2-oxobutanoate hydroxymethyltransferase OS=Populus trichocarpa GN=POPTR... [more]
A0A067KNV3_JATCU5.7e-5375.523-methyl-2-oxobutanoate hydroxymethyltransferase OS=Jatropha curcas GN=JCGZ_0788... [more]
D7LSB0_ARALL1.3e-5275.743-methyl-2-oxobutanoate hydroxymethyltransferase OS=Arabidopsis lyrata subsp. ly... [more]
A0A087H5I9_ARAAL3.7e-5276.093-methyl-2-oxobutanoate hydroxymethyltransferase OS=Arabis alpina GN=AALP_AA4G25... [more]
BLAST of MU66156 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G46110.14.1e-5575.36 ketopantoate hydroxymethyltransferase 1[more]
AT3G61530.15.1e-5375.38 Phosphoenolpyruvate carboxylase family protein[more]
BLAST of MU66156 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659092903|ref|XP_008447278.1|1.1e-7098.60PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo][more]
gi|449443772|ref|XP_004139651.1|1.4e-6895.10PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
gi|659098169|ref|XP_008450010.1|3.3e-6290.00PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase-like [Cucumis melo][more]
gi|698473793|ref|XP_009784538.1|1.6e-5380.92PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial-lik... [more]
gi|697122926|ref|XP_009615453.1|6.2e-5380.15PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial-lik... [more]
Annotated Terms
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR003700Pantoate_hydroxy_MeTrfase
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
Vocabulary: Molecular Function
TermDefinition
GO:00038643-methyl-2-oxobutanoate hydroxymethyltransferase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0015940pantothenate biosynthetic process
GO Annotation
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015940 pantothenate biosynthetic process
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0003824 catalytic activity
Relationships

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
MELO3C012767MELO3C012767gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
TEa0010G15TEa0010G15EST


InterPro
Analysis Name: InterPro Annotations of melon unigene v4
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 47..168
score: 7.9
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 71..169
score: 5.1
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainGENE3DG3DSA:3.20.20.60coord: 70..169
score: 3.5
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domainunknownSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 71..168
score: 2.98
NoneNo IPR availablePANTHERPTHR20881:SF03-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 47..168
score: 7.9