MU55386 (transcribed_cluster) Melon (DHL92) v3.5.1

NameMU55386
Typetranscribed_cluster
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionProtein N-terminal asparagine amidohydrolase
Locationchr4 : 24442307 .. 24445290 (-)
Sequence length811
The following sequences are available for this feature:

transcribed_cluster sequence

GATTCTCCTTTGCTATAATATGAACATGAATAAAGAAAAGAAAAACCCCATGAAAACCCGTTTTTTTTCCTCAAACAATTCATTGCAAAGTTTCCACTGATTGGTTCTTTCTCCTTGCACAAATTTCTTCTCTTCTTCTCTTCTTCTCTTCTTCCCTCTCCCATTTGTTCTTCCTTCGTCTACCATCTCCTTCAAGCTTCCATACCAGAACCATGATTTTCGTCGATGGCGCACCCTGCACCCTCCAATCATCTTCGTCAAATAAGGACATCTGTTGCACATATGGATTTCCCAGACATCATCAATATTGCCCTCTCACAGATGTTATCCCTAGTTGTCGATCCCACTGCTGATGCTGAGTTAGATGTTCATTTAGTTGGAGGTTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGGACCCCTAACTTCGTGATTGCTTTCCAAGGATGCTATCCACAAAATCAGAATAGTTGAATTGAATAAATTGGGGTTTGATGCAGGAAAATAATAATATTACTCGGACAGGAGATCGTAAAAAGATGGAGGGCTATTCGTTACCGCTGTGTAATAAAATTATAAGGAGTTTGTGGACAAGACCCGAGAAATTTCATCTTCAGACTCTTTGTATTCTTCTGCATAACACTCGGAGAGACTCTCACGGAAATTCTTACCCTATCTTTAATGGATTCGCGGTGAAACATCTGACGGATCTGTGTTCCCCGCTAGCTTTGATAGTACTTCAAGATGCCCAGATGAAGTTGTTCGGAGAATTCGACTGTCTTCATCATATGAAGATCCAAGTTGGGAGGGC
Library categoryESTs
melon 15d library1
BLAST of MU55386 vs. TrEMBL
Match: A0A0A0L9W7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G702610 PE=4 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-29
Identity = 63/69 (91.30%), Postives = 65/69 (94.20%), Query Frame = 3

Query: 492 LMQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 551
           L++ENNNITR GDRKKMEGYSLPLCNKII SLWTRPEKFHLQTLCIL HNTRRDS GNSY
Sbjct: 146 LLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTRPEKFHLQTLCILQHNTRRDSEGNSY 205

Query: 552 PIFNGFAVK 611
           PIFNGFAVK
Sbjct: 206 PIFNGFAVK 214

Query: 612 671
           
Sbjct: 266 214

Query: 672 699
           
Sbjct: 326 214

BLAST of MU55386 vs. TrEMBL
Match: A0A0A0L9W7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G702610 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.4e-15
Identity = 45/46 (97.83%), Postives = 45/46 (97.83%), Query Frame = 1

Query: 268 TSVAHMDFPDIINIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 327
           TSVAHMDFPDII IALSQMLSLVVDPTADAELDVHLVGGFEDVLLK
Sbjct: 103 TSVAHMDFPDIIQIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 148

Query: 328 387
           
Sbjct: 163 148

Query: 388 406
           
Sbjct: 223 148


HSP 2 Score: 82.0 bits (201), Expect = 1.2e-12
Identity = 37/39 (94.87%), Postives = 39/39 (100.00%), Query Frame = 2

Query: 695 ETSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEG 754
           +TSDGSVFPASFDSTSRCPDE+VRRIRLSSSYEDPSWEG
Sbjct: 214 KTSDGSVFPASFDSTSRCPDEIVRRIRLSSSYEDPSWEG 252

Query: 755 812
           
Sbjct: 274 252


HSP 3 Score: 35.8 bits (81), Expect = 9.8e+01
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3

Query: 213 MIFVDGAPCTLQSSSSNK 267
           MIFVDGAP TLQSSSSNK
Sbjct: 1   MIFVDGAPFTLQSSSSNK 18


HSP 4 Score: 91.7 bits (226), Expect = 1.5e-15
Identity = 39/76 (51.32%), Postives = 53/76 (69.74%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q+ N    +     ++GYS PLC+KI+ +LW+R EKFHLQT C+L HNTRRDS GN+YP
Sbjct: 132 LQQGNGSAVSESPAYLDGYSFPLCSKIVHTLWSRDEKFHLQTFCVLGHNTRRDSDGNTYP 191

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF V+  + +  P
Sbjct: 192 FFNGFVVETASGIVIP 207

Query: 615 674
           
Sbjct: 252 207

Query: 675 723
           
Sbjct: 312 207

BLAST of MU55386 vs. TrEMBL
Match: A0A0L9UJ97_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan05g045200 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-05
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = 2

Query: 695 ETSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWE 754
           ET+ G V PA FD TSRCPDE+VRRIR+S SYED +W+
Sbjct: 199 ETASGIVIPAIFDRTSRCPDELVRRIRVSVSYEDANWK 236

Query: 755 809
           
Sbjct: 259 236


HSP 2 Score: 58.5 bits (140), Expect = 1.4e-05
Identity = 28/46 (60.87%), Postives = 35/46 (76.09%), Query Frame = 1

Query: 268 TSVAHMDFPDIINIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 327
           TSVAHMD P I+ I LSQMLS +V  + + E +VHL+GGFEDV L+
Sbjct: 88  TSVAHMDSPKIVEIGLSQMLSSLVANSLETEFEVHLIGGFEDVSLQ 133

Query: 328 387
           
Sbjct: 148 133

Query: 388 406
           
Sbjct: 208 133


HSP 3 Score: 91.7 bits (226), Expect = 1.5e-15
Identity = 39/76 (51.32%), Postives = 53/76 (69.74%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q+ N    +     ++GYS PLC+KI+ +LW+R EKFHLQT C+L HNTRRDS GN+YP
Sbjct: 146 LQQGNGSAVSESPAYLDGYSFPLCSKIVHTLWSRDEKFHLQTFCVLGHNTRRDSDGNTYP 205

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF V+  + +  P
Sbjct: 206 FFNGFVVETASGIVIP 221

Query: 615 674
           
Sbjct: 266 221

Query: 675 723
           
Sbjct: 326 221

BLAST of MU55386 vs. TrEMBL
Match: A0A0S3SJP4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.07G193500 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-05
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = 2

Query: 695 ETSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWE 754
           ET+ G V PA FD TSRCPDE+VRRIR+S SYED +W+
Sbjct: 213 ETASGIVIPAIFDRTSRCPDELVRRIRVSVSYEDANWK 250

Query: 755 809
           
Sbjct: 273 250


HSP 2 Score: 58.5 bits (140), Expect = 1.4e-05
Identity = 28/46 (60.87%), Postives = 35/46 (76.09%), Query Frame = 1

Query: 268 TSVAHMDFPDIINIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 327
           TSVAHMD P I+ I LSQMLS +V  + + E +VHL+GGFEDV L+
Sbjct: 102 TSVAHMDSPKIVEIGLSQMLSSLVANSLETEFEVHLIGGFEDVSLQ 147

Query: 328 387
           
Sbjct: 162 147

Query: 388 406
           
Sbjct: 222 147


HSP 3 Score: 90.1 bits (222), Expect = 4.4e-15
Identity = 38/76 (50.00%), Postives = 55/76 (72.37%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q  N  T +     ++G+S PLC+KI+ +LW+R EKFH++T+C+L HNTRRDS+GN+YP
Sbjct: 114 LQHANGGTVSESPVHLDGHSFPLCSKIVHTLWSREEKFHIRTICVLGHNTRRDSNGNTYP 173

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF V+  T +  P
Sbjct: 174 FFNGFVVETTTGIIIP 189

Query: 615 674
           
Sbjct: 234 189

Query: 675 723
           
Sbjct: 294 189

BLAST of MU55386 vs. TrEMBL
Match: A0A151SWY0_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_014715 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.8e-07
Identity = 27/39 (69.23%), Postives = 32/39 (82.05%), Query Frame = 2

Query: 695 ETSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEG 754
           ET+ G + PA FD TSRCPDE+VRRIR+S+SYED SW G
Sbjct: 181 ETTTGIIIPAIFDRTSRCPDEIVRRIRVSASYEDASWRG 219

Query: 755 812
           
Sbjct: 241 219


HSP 2 Score: 60.8 bits (146), Expect = 2.9e-06
Identity = 28/46 (60.87%), Postives = 37/46 (80.43%), Query Frame = 1

Query: 268 TSVAHMDFPDIINIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 327
           TSVAH+D P I+ + LSQ+LS +VD + + ELDVHL+GGFEDV L+
Sbjct: 70  TSVAHLDSPKIVEMGLSQILSSLVDNSLETELDVHLIGGFEDVSLQ 115

Query: 328 387
           
Sbjct: 130 115

Query: 388 406
           
Sbjct: 190 115


HSP 3 Score: 89.4 bits (220), Expect = 7.5e-15
Identity = 38/76 (50.00%), Postives = 52/76 (68.42%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q  N  T +     ++GYS PLC KI+ +LW+R EKFH++T+C+L HNTRRDS GN+YP
Sbjct: 143 LQHANGSTVSESPADLDGYSFPLCLKIVHTLWSREEKFHIRTICVLGHNTRRDSDGNTYP 202

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF  +  T +  P
Sbjct: 203 FFNGFVAETTTGIIIP 218

Query: 615 674
           
Sbjct: 263 218

Query: 675 723
           
Sbjct: 323 218

BLAST of MU55386 vs. TAIR10
Match: AT2G44420.1 (AT2G44420.1 protein N-terminal asparagine amidohydrolase family protein)

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-12
Identity = 36/71 (50.70%), Postives = 40/71 (56.34%), Query Frame = 3

Query: 510 NITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYPIFNGF 569
           N    GD  K EGYS PLC K++ +L  R E FH+QTL IL HNT+ DS  N+ PIFNG 
Sbjct: 153 NADGVGDYAKPEGYSFPLCCKLVETLQKRRENFHIQTLFILGHNTKLDSQANTCPIFNGC 212

Query: 570 AVKHLTDLCSP 629
            V   T    P
Sbjct: 213 LVNTSTGAILP 223

Query: 630 689
           
Sbjct: 273 223

Query: 690 723
           
Sbjct: 333 223


HSP 2 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 29/46 (63.04%), Postives = 36/46 (78.26%), Query Frame = 1

Query: 268 TSVAHMDFPDIINIALSQMLSLVVDPTADAELDVHLVGGFEDVLLK 327
           TSVAHMD P+I+++ +SQML LV+    DAELDVH+VGG+EDV +K
Sbjct: 107 TSVAHMDSPEIVDLGISQMLLLVLQDDVDAELDVHMVGGYEDVDIK 152

Query: 328 387
           
Sbjct: 167 152

Query: 388 406
           
Sbjct: 227 152


HSP 3 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 27/38 (71.05%), Postives = 32/38 (84.21%), Query Frame = 2

Query: 698 TSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEG 757
           TS G++ PASF+ TSRCPDE+VRRIR+SSS+ED SW+G
Sbjct: 216 TSTGAILPASFNRTSRCPDEIVRRIRVSSSFEDSSWKG 253

Query: 758 812
           
Sbjct: 276 253

BLAST of MU55386 vs. NCBI nr
Match: gi|659123648|ref|XP_008461769.1| (PREDICTED: protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo])

HSP 1 Score: 148.7 bits (374), Expect = 1.5e-32
Identity = 67/69 (97.10%), Postives = 69/69 (100.00%), Query Frame = 3

Query: 492 LMQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 551
           L++ENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY
Sbjct: 146 LLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 205

Query: 552 PIFNGFAVK 611
           PIFNGFAVK
Sbjct: 206 PIFNGFAVK 214

Query: 612 671
           
Sbjct: 266 214

Query: 672 699
           
Sbjct: 326 214

BLAST of MU55386 vs. NCBI nr
Match: gi|659123650|ref|XP_008461770.1| (PREDICTED: protein N-terminal asparagine amidohydrolase isoform X2 [Cucumis melo])

HSP 1 Score: 148.7 bits (374), Expect = 1.5e-32
Identity = 67/69 (97.10%), Postives = 69/69 (100.00%), Query Frame = 3

Query: 492 LMQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 551
           L++ENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY
Sbjct: 84  LLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 143

Query: 552 PIFNGFAVK 611
           PIFNGFAVK
Sbjct: 144 PIFNGFAVK 152

Query: 612 671
           
Sbjct: 204 152

Query: 672 699
           
Sbjct: 264 152

BLAST of MU55386 vs. NCBI nr
Match: gi|449463783|ref|XP_004149611.1| (PREDICTED: protein N-terminal asparagine amidohydrolase [Cucumis sativus])

HSP 1 Score: 138.3 bits (347), Expect = 2.0e-29
Identity = 63/69 (91.30%), Postives = 65/69 (94.20%), Query Frame = 3

Query: 492 LMQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSY 551
           L++ENNNITR GDRKKMEGYSLPLCNKII SLWTRPEKFHLQTLCIL HNTRRDS GNSY
Sbjct: 146 LLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTRPEKFHLQTLCILQHNTRRDSEGNSY 205

Query: 552 PIFNGFAVK 611
           PIFNGFAVK
Sbjct: 206 PIFNGFAVK 214

Query: 612 671
           
Sbjct: 266 214

Query: 672 699
           
Sbjct: 326 214

BLAST of MU55386 vs. NCBI nr
Match: gi|950965054|ref|XP_014498746.1| (PREDICTED: protein N-terminal asparagine amidohydrolase isoform X4 [Vigna radiata var. radiata])

HSP 1 Score: 94.4 bits (233), Expect = 3.3e-16
Identity = 39/76 (51.32%), Postives = 53/76 (69.74%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q+ N    +     ++GYS PLC+KI+ +LW+R EKFHLQT C+L HNTRRDS GN+YP
Sbjct: 152 LQQGNGSAISESPAYLDGYSFPLCSKIVHTLWSRDEKFHLQTFCVLGHNTRRDSDGNTYP 211

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF V+  + +  P
Sbjct: 212 FFNGFVVETASGIVIP 227

Query: 615 674
           
Sbjct: 272 227

Query: 675 723
           
Sbjct: 332 227

BLAST of MU55386 vs. NCBI nr
Match: gi|920699623|gb|KOM42848.1| (hypothetical protein LR48_Vigan05g045200 [Vigna angularis])

HSP 1 Score: 94.4 bits (233), Expect = 3.3e-16
Identity = 39/76 (51.32%), Postives = 53/76 (69.74%), Query Frame = 3

Query: 495 MQENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTRRDSHGNSYP 554
           +Q+ N    +     ++GYS PLC+KI+ +LW+R EKFHLQT C+L HNTRRDS GN+YP
Sbjct: 132 LQQGNGSAVSESPAYLDGYSFPLCSKIVHTLWSRDEKFHLQTFCVLGHNTRRDSDGNTYP 191

Query: 555 IFNGFAVKHLTDLCSP 614
            FNGF V+  + +  P
Sbjct: 192 FFNGFVVETASGIVIP 207

Query: 615 674
           
Sbjct: 252 207

Query: 675 723
           
Sbjct: 312 207

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0A0L9W7_CUCSA7.0e-2991.30Uncharacterized protein OS=Cucumis sativus GN=Csa_3G702610 PE=4 SV=1[more]
A0A0A0L9W7_CUCSA4.4e-1597.83Uncharacterized protein OS=Cucumis sativus GN=Csa_3G702610 PE=4 SV=1[more]
A0A0L9UJ97_PHAAN1.1e-0568.42Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan05g045200 PE=4 SV=1[more]
A0A0S3SJP4_PHAAN1.1e-0568.42Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.07G193500 PE=... [more]
A0A151SWY0_CAJCA5.8e-0769.23Uncharacterized protein OS=Cajanus cajan GN=KK1_014715 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44420.11.4e-1250.70 protein N-terminal asparagine amidohydrolase family protein[more]
Match NameE-valueIdentityDescription
gi|659123648|ref|XP_008461769.1|1.5e-3297.10PREDICTED: protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo... [more]
gi|659123650|ref|XP_008461770.1|1.5e-3297.10PREDICTED: protein N-terminal asparagine amidohydrolase isoform X2 [Cucumis melo... [more]
gi|449463783|ref|XP_004149611.1|2.0e-2991.30PREDICTED: protein N-terminal asparagine amidohydrolase [Cucumis sativus][more]
gi|950965054|ref|XP_014498746.1|3.3e-1651.32PREDICTED: protein N-terminal asparagine amidohydrolase isoform X4 [Vigna radiat... [more]
gi|920699623|gb|KOM42848.1|3.3e-1651.32hypothetical protein LR48_Vigan05g045200 [Vigna angularis][more]
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR026750NTAN1
Vocabulary: Molecular Function
TermDefinition
GO:0008418protein-N-terminal asparagine amidohydrolase activity
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008418 protein-N-terminal asparagine amidohydrolase activity

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
MELO3C023943MELO3C023943gene
MELO3C023942MELO3C023942gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
15D32F0815D32F08EST


Analysis Name: InterPro Annotations of melon unigene v4
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026750Protein N-terminal asparagine amidohydrolasePFAMPF14736N_Asn_amidohydcoord: 11..57
score: 3.