CU177991 (transcribed_cluster) Cucumber (Chinese Long) v2

Overview
NameCU177991
Typetranscribed_cluster
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionMalate synthase
LocationChr1 : 5873836 .. 5874313 (+)
Sequence length481
Genome Viewer
Sequences
The following sequences are available for this feature:

transcribed_cluster sequence

NGGTAGCAGGGTTCAAAACTGGCAATGGCTGAAGTATGGAGTGGAATTGGATGGAGATGGGCTTGGTGTGAGAGTGAACAAGGAACTGTTTGGGAGAGTGGTGGAAGAAGAAATGGAAAGGATTGAAAGAGAAGTTGGGAAAGAGAGATTCAAAAAAGGAATGTACAAAGAAGCTTGCAAGATGTTCACAAGGCAATGCACAGCTCCAAATTTGGATGACTTTCTGACCTTAGACGCTTACAACTATATTGTTATACATCATCCAAGGGAATTGTCCAAGCTTTGAAAAGTGAAAACCACCACCAAAACACTTGCTTTTGCTTCCATTCTCTTCTTGTTACTGAGAATAACGTATCTGTAATGTAAGTCTGTCTTTGTCCATTTCTTTTTGCAAGCTGTGTGGTTATTTCAAGAAATCCAGCAATTGCAAGCAAATTGGAATTCGGTACTTCTCTATATATAAACCTATATTTTGCCTGTT
Library
Library categoryESTs
published gene (cucumber)1
Homology
[Close]
BLAST of CU177991 vs. Swiss-Prot
Match: MASY_CUCSA (Malate synthase, glyoxysomal OS=Cucumis sativus PE=2 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 4.8e-39
Identity = 77/91 (84.62%), Postives = 75/91 (82.42%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACKM
Sbjct: 476 SRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKM 535

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 536 FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 566

Query: 125  184
           
Sbjct: 596  566

Query: 185  244
           
Sbjct: 656  566

Query: 245  278
           
Sbjct: 716  566

[Close]
BLAST of CU177991 vs. Swiss-Prot
Match: MASY_CUCMA (Malate synthase, glyoxysomal OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.3e-36
Identity = 72/91 (79.12%), Postives = 73/91 (80.22%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELF               GKE+F+KGMYKEACKM
Sbjct: 474 SRVQNWQWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKM 533

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAP LDDFLTLDAYN+IVIHHPRELS
Sbjct: 534 FTRQCTAPTLDDFLTLDAYNHIVIHHPRELS 564

Query: 125  184
           
Sbjct: 594  564

Query: 185  244
           
Sbjct: 654  564

Query: 245  278
           
Sbjct: 714  564

[Close]
BLAST of CU177991 vs. Swiss-Prot
Match: MASY_SOYBN (Malate synthase, glyoxysomal (Fragment) OS=Glycine max PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.6e-34
Identity = 67/91 (73.63%), Postives = 72/91 (79.12%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVEL+GDGLGV+VNKELFG              G E+FK+GMYKEACK+
Sbjct: 472 SRVQNWQWLKYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKEGMYKEACKI 531

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCT+P LDDFLTLDAYNYIV+HHPRE S
Sbjct: 532 FTRQCTSPMLDDFLTLDAYNYIVVHHPRETS 562

Query: 125  184
           
Sbjct: 592  562

Query: 185  244
           
Sbjct: 652  562

Query: 245  278
           
Sbjct: 712  562

[Close]
BLAST of CU177991 vs. Swiss-Prot
Match: MASY_GOSHI (Malate synthase, glyoxysomal OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-31
Identity = 66/91 (72.53%), Postives = 70/91 (76.92%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQ WQWLKYGVELDGDGLGVRVN  +FG              GKE+FKKGMYKEACK+
Sbjct: 475 SRVQIWQWLKYGVELDGDGLGVRVN-HVFGRVVEEEMARIEREVGKEKFKKGMYKEACKI 534

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTA  LDDFLTLDAYNYIVIHHP+++S
Sbjct: 535 FTRQCTASTLDDFLTLDAYNYIVIHHPKDVS 564

Query: 125  184
           
Sbjct: 595  564

Query: 185  244
           
Sbjct: 655  564

Query: 245  278
           
Sbjct: 715  564

[Close]
BLAST of CU177991 vs. Swiss-Prot
Match: MASY_ARATH (Malate synthase OS=Arabidopsis thaliana GN=MLS PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.8e-30
Identity = 61/91 (67.03%), Postives = 68/91 (74.73%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQW++YGVELDGDGLGVRV+KELFG              GK++FK GMYKEACKM
Sbjct: 470 SRVQNWQWIRYGVELDGDGLGVRVSKELFGRVVEEEMERIEKEVGKDKFKNGMYKEACKM 529

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FT+QCTAP LDDFLTL  YN+IV H+P  +S
Sbjct: 530 FTKQCTAPELDDFLTLAVYNHIVAHYPINVS 560

Query: 125  184
           
Sbjct: 590  560

Query: 185  244
           
Sbjct: 650  560

Query: 245  278
           
Sbjct: 710  560

[Close]
BLAST of CU177991 vs. TrEMBL
Match: A0A0A0LUC5_CUCSA (Malate synthase OS=Cucumis sativus GN=Csa_1G050360 PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.4e-37
Identity = 77/91 (84.62%), Postives = 77/91 (84.62%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACKM
Sbjct: 476 SRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKM 535

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 536 FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 566

Query: 125  184
           
Sbjct: 596  566

Query: 185  244
           
Sbjct: 656  566

Query: 245  278
           
Sbjct: 716  566

[Close]
BLAST of CU177991 vs. TrEMBL
Match: E3UZU8_CUCMA (Malate synthase OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-34
Identity = 72/91 (79.12%), Postives = 75/91 (82.42%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELF               GKE+F+KGMYKEACKM
Sbjct: 475 SRVQNWQWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKM 534

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAP LDDFLTLDAYN+IVIHHPRELS
Sbjct: 535 FTRQCTAPTLDDFLTLDAYNHIVIHHPRELS 565

Query: 125  184
           
Sbjct: 595  565

Query: 185  244
           
Sbjct: 655  565

Query: 245  278
           
Sbjct: 715  565

[Close]
BLAST of CU177991 vs. TrEMBL
Match: A0A0B2Q284_GLYSO (Malate synthase OS=Glycine soja GN=glysoja_033315 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 8.1e-33
Identity = 68/91 (74.73%), Postives = 74/91 (81.32%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVEL+GDGLGV+VNKELFG              G E+FKKGMYKEACK+
Sbjct: 477 SRVQNWQWLKYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKKGMYKEACKI 536

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCT+P LDDFLTLDAYNYIV+HHPRE S
Sbjct: 537 FTRQCTSPTLDDFLTLDAYNYIVVHHPRETS 567

Query: 125  184
           
Sbjct: 597  567

Query: 185  244
           
Sbjct: 657  567

Query: 245  278
           
Sbjct: 717  567

[Close]
BLAST of CU177991 vs. TrEMBL
Match: I1MUM7_SOYBN (Malate synthase OS=Glycine max GN=GLYMA_17G128000 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.4e-32
Identity = 68/91 (74.73%), Postives = 74/91 (81.32%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVEL+GDGLGV+VNKELFG              G E+FKKGMYKEACK+
Sbjct: 475 SRVQNWQWLKYGVELNGDGLGVKVNKELFGRVVEEEMARIEKEVGTEKFKKGMYKEACKI 534

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCT+P LDDFLTLDAYNYIV+HHPRE S
Sbjct: 535 FTRQCTSPMLDDFLTLDAYNYIVVHHPRETS 565

Query: 125  184
           
Sbjct: 595  565

Query: 185  244
           
Sbjct: 655  565

Query: 245  278
           
Sbjct: 715  565

[Close]
BLAST of CU177991 vs. TrEMBL
Match: G7ZYR8_MEDTR (Malate synthase OS=Medicago truncatula GN=MTR_8g028225 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.4e-31
Identity = 66/91 (72.53%), Postives = 74/91 (81.32%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGV+V+KELFG              GK++FKKGMYK+ACK+
Sbjct: 475 SRVQNWQWLKYGVELDGDGLGVKVSKELFGRVVEEEMDRIEKEVGKDKFKKGMYKDACKI 534

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCT+P LDDFLTLDAYNYIV+ HP ELS
Sbjct: 535 FTRQCTSPTLDDFLTLDAYNYIVVVHPVELS 565

Query: 125  184
           
Sbjct: 595  565

Query: 185  244
           
Sbjct: 655  565

Query: 245  278
           
Sbjct: 715  565

[Close]
BLAST of CU177991 vs. NCBI nr
Match: gi|167521|gb|AAA33123.1| (glyoxysomal malate synthase (EC 4.1.3.1), partial [Cucumis sativus])

HSP 1 Score: 166.4 bits (420), Expect = 4.1e-38
Identity = 78/92 (84.78%), Postives = 78/92 (84.78%), Query Frame = 2 

Query: 2   GSRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACK 61
           GSRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACK
Sbjct: 1   GSRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACK 60

Query: 62  MFTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 121
           MFTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 61  MFTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 92

Query: 122  181
           
Sbjct: 121  92

Query: 182  241
           
Sbjct: 181  92

Query: 242  278
           
Sbjct: 241  92

[Close]
BLAST of CU177991 vs. NCBI nr
Match: gi|126768|sp|P08216.2|MASY_CUCSA (RecName: Full=Malate synthase, glyoxysomal [Cucumis sativus])

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-37
Identity = 77/91 (84.62%), Postives = 77/91 (84.62%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACKM
Sbjct: 476 SRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKM 535

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 536 FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 566

Query: 125  184
           
Sbjct: 596  566

Query: 185  244
           
Sbjct: 656  566

Query: 245  278
           
Sbjct: 716  566

[Close]
BLAST of CU177991 vs. NCBI nr
Match: gi|659067451|ref|XP_008439505.1| (PREDICTED: malate synthase, glyoxysomal [Cucumis melo])

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-37
Identity = 77/91 (84.62%), Postives = 77/91 (84.62%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACKM
Sbjct: 476 SRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKM 535

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 536 FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 566

Query: 125  184
           
Sbjct: 596  566

Query: 185  244
           
Sbjct: 656  566

Query: 245  278
           
Sbjct: 716  566

[Close]
BLAST of CU177991 vs. NCBI nr
Match: gi|449469625|ref|XP_004152519.1| (PREDICTED: malate synthase, glyoxysomal [Cucumis sativus])

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-37
Identity = 77/91 (84.62%), Postives = 77/91 (84.62%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELFG              GKERFKKGMYKEACKM
Sbjct: 476 SRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIEREVGKERFKKGMYKEACKM 535

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS
Sbjct: 536 FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 566

Query: 125  184
           
Sbjct: 596  566

Query: 185  244
           
Sbjct: 656  566

Query: 245  278
           
Sbjct: 716  566

[Close]
BLAST of CU177991 vs. NCBI nr
Match: gi|310697396|gb|ADP06653.1| (malate synthase [Cucurbita maxima])

HSP 1 Score: 155.6 bits (392), Expect = 7.2e-35
Identity = 72/91 (79.12%), Postives = 75/91 (82.42%), Query Frame = 2 

Query: 5   SRVQNWQWLKYGVELDGDGLGVRVNKELFGXXXXXXXXXXXXXXGKERFKKGMYKEACKM 64
           SRVQNWQWLKYGVELDGDGLGVRVNKELF               GKE+F+KGMYKEACKM
Sbjct: 475 SRVQNWQWLKYGVELDGDGLGVRVNKELFARVVEEEMERIEREVGKEKFRKGMYKEACKM 534

Query: 65  FTRQCTAPNLDDFLTLDAYNYIVIHHPRELS 124
           FTRQCTAP LDDFLTLDAYN+IVIHHPRELS
Sbjct: 535 FTRQCTAPTLDDFLTLDAYNHIVIHHPRELS 565

Query: 125  184
           
Sbjct: 595  565

Query: 185  244
           
Sbjct: 655  565

Query: 245  278
           
Sbjct: 715  565

The following BLAST results are available for this feature:
BLAST of CU177991 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MASY_CUCSA4.8e-3984.62Malate synthase, glyoxysomal OS=Cucumis sativus PE=2 SV=2[more]
MASY_CUCMA2.3e-3679.12Malate synthase, glyoxysomal OS=Cucurbita maxima PE=1 SV=1[more]
MASY_SOYBN3.6e-3473.63Malate synthase, glyoxysomal (Fragment) OS=Glycine max PE=2 SV=1[more]
MASY_GOSHI1.3e-3172.53Malate synthase, glyoxysomal OS=Gossypium hirsutum PE=2 SV=1[more]
MASY_ARATH1.8e-3067.03Malate synthase OS=Arabidopsis thaliana GN=MLS PE=1 SV=1[more]
BLAST of CU177991 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LUC5_CUCSA5.4e-3784.62Malate synthase OS=Cucumis sativus GN=Csa_1G050360 PE=3 SV=1[more]
E3UZU8_CUCMA2.5e-3479.12Malate synthase OS=Cucurbita maxima PE=2 SV=1[more]
A0A0B2Q284_GLYSO8.1e-3374.73Malate synthase OS=Glycine soja GN=glysoja_033315 PE=3 SV=1[more]
I1MUM7_SOYBN1.4e-3274.73Malate synthase OS=Glycine max GN=GLYMA_17G128000 PE=3 SV=1[more]
G7ZYR8_MEDTR3.4e-3172.53Malate synthase OS=Medicago truncatula GN=MTR_8g028225 PE=3 SV=1[more]
BLAST of CU177991 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|167521|gb|AAA33123.1|4.1e-3884.78glyoxysomal malate synthase (EC 4.1.3.1), partial [Cucumis sativus][more]
gi|126768|sp|P08216.2|MASY_CUCSA1.6e-3784.62RecName: Full=Malate synthase, glyoxysomal [Cucumis sativus][more]
gi|659067451|ref|XP_008439505.1|1.6e-3784.62PREDICTED: malate synthase, glyoxysomal [Cucumis melo][more]
gi|449469625|ref|XP_004152519.1|1.6e-3784.62PREDICTED: malate synthase, glyoxysomal [Cucumis sativus][more]
gi|310697396|gb|ADP06653.1|7.2e-3579.12malate synthase [Cucurbita maxima][more]
Annotated Terms
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR001465Malate_synthase
IPR011076Malate_synth-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004474malate synthase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006097glyoxylate cycle
GO Annotation
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006097 glyoxylate cycle
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004474 malate synthase activity
Relationships

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Csa1G050360Csa1G050360gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
M16219M16219EST


InterPro
Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001465Malate synthasePFAMPF01274Malate_synthasecoord: 3..85
score: 4.8
IPR011076Malate synthase-likeunknownSSF51645Malate synthase Gcoord: 3..85
score: 3.01
NoneNo IPR availableGENE3DG3DSA:1.20.1220.12coord: 3..78
score: 2.2
NoneNo IPR availablePANTHERPTHR21631ISOCITRATE LYASE/MALATE SYNTHASEcoord: 3..93
score: 3.4
NoneNo IPR availablePANTHERPTHR21631:SF1MALATE SYNTHASE 1, GLYOXYSOMAL-RELATEDcoord: 3..93
score: 3.4