CU165969 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU165969
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Aquaporin NIP1-1
Location	Chr3 : 18051388 .. 18052084 (+)
Sequence length	229

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GGATCTCAGAATCAATGGCCACAAAGATCGACGGAATCGAAGATGAAGAAATTTCAAAGCTTGAAGAAGGCATTGTCGTTTCCGCCGTTGCTCGTTTCTGTCCCTCCAATTTAGTCGTCATCATCCAAAAGGTAATCGCGGAGTTGATCGGGACGTACTTCGTGATCTTCGGGGGATGTGGGGCGGTGGTGGTGAACAAAATATACGGATCAGTGACATTTCCGGGAAT

Library


Library category	ESTs
hermaphrodite flower library	1

Homology

[Close]

BLAST of CU165969 vs. Swiss-Prot
Match: NIP1_NICAL (Probable aquaporin NIP-type OS=Nicotiana alata PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 9.3e-17
Identity = 47/74 (63.51%), Postives = 55/74 (74.32%), Query Frame = 3


Query: 24  KIDGIEDEEISKLEEGIVVSAV-----ARFCPS-NLVVIIQKVIAELIGTYFVIFGGCGA 83
           K DG ++EEIS++EEG + SA        FC S ++VVI+QK+IAE IGTYFVIF GCG+
Sbjct: 3   KKDGNQEEEISQMEEGNIHSASNSDSNVGFCSSVSVVVILQKLIAEAIGTYFVIFAGCGS 62

Query: 84  VVVNKIYGSVTFPG 143
           V VNKIYGSVTFPG
Sbjct: 63  VAVNKIYGSVTFPG 76

Query: 144  203
           
Sbjct: 123  76

Query: 204  228
           
Sbjct: 183  76

[Close]

BLAST of CU165969 vs. Swiss-Prot
Match: NIP41_ARATH (Putative aquaporin NIP4-1 OS=Arabidopsis thaliana GN=NIP4-1 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.4e-11
Identity = 37/78 (47.44%), Postives = 52/78 (66.67%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIV------VSAVARFCPSNLVVIIQKVIAELIGTYFVIFGG 74
           M++  D IE+E+IS++E+G        +  V    PS +V + QK+IAE+IGTYF++F G
Sbjct: 1   MSSHSDEIEEEQISRIEKGKGKDCQGGIETVICTSPS-IVCLTQKLIAEMIGTYFIVFSG 60

Query: 75  CGAVVVNKIY-GSVTFPG 134
           CG VVVN +Y G++TFPG
Sbjct: 61  CGVVVVNVLYGGTITFPG 77

Query: 135  194
           
Sbjct: 121  77

Query: 195  228
           
Sbjct: 181  77

[Close]

BLAST of CU165969 vs. Swiss-Prot
Match: NIP42_ARATH (Probable aquaporin NIP4-2 OS=Arabidopsis thaliana GN=NIP4-2 PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 3.4e-11
Identity = 37/77 (48.05%), Postives = 51/77 (66.23%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVA----RFCPS-NLVVIIQKVIAELIGTYFVIFGGC 74
           M +  + IEDE+IS++E+G    +        C S ++V + QK+IAE+IGTYF+IF GC
Sbjct: 1   MTSHGEEIEDEQISRIEKGNCKDSQGGMETAICSSPSIVCLTQKLIAEMIGTYFIIFSGC 60

Query: 75  GAVVVNKIY-GSVTFPG 134
           G VVVN +Y G++TFPG
Sbjct: 61  GVVVVNVLYGGTITFPG 77

Query: 135  194
           
Sbjct: 121  77

Query: 195  228
           
Sbjct: 181  77

[Close]

BLAST of CU165969 vs. Swiss-Prot
Match: NIP11_ORYSJ (Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-06
Identity = 24/39 (61.54%), Postives = 28/39 (71.79%), Query Frame = 3


Query: 114 VVIIQKVIAELIGTYFVIFGGCGAVVVNKI-YGSVTFPG 173
           V  IQK+IAE+ GTYF+IF GCGAV +N+   G +TFPG
Sbjct: 44  VPFIQKIIAEIFGTYFLIFAGCGAVTINQSKNGQITFPG 82

Query: 174  228
           
Sbjct: 104  82

[Close]

BLAST of CU165969 vs. Swiss-Prot
Match: NIP11_MAIZE (Aquaporin NIP1-1 OS=Zea mays GN=NIP1-1 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-06
Identity = 28/59 (47.46%), Postives = 36/59 (61.02%), Query Frame = 3


Query: 60  LEEGIVVSAVARFCPSNLVVI--IQKVIAELIGTYFVIFGGCGAVVVN-KIYGSVTFPG 119
           LEEG       + C + +V +  IQK+IAE+ GTYF++F GCGAV +N    G +TFPG
Sbjct: 20  LEEGRKEEFADQGCAAMVVSVPFIQKIIAEIFGTYFLMFAGCGAVTINASKNGQITFPG 78

Query: 120  179
           
Sbjct: 80   78

Query: 180  228
           
Sbjct: 140  78

[Close]

BLAST of CU165969 vs. TrEMBL
Match: A0A0A0LA39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G345890 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 6.0e-31
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 74
           MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV
Sbjct: 1   MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 60

Query: 75  NKIYGSVTFPG 134
           NKIYGSVTFPG
Sbjct: 61  NKIYGSVTFPG 71

Query: 135  194
           
Sbjct: 121  71

Query: 195  228
           
Sbjct: 181  71

[Close]

BLAST of CU165969 vs. TrEMBL
Match: A0A151U148_CAJCA (Putative aquaporin NIP-type OS=Cajanus cajan GN=KK1_005646 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.9e-17
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVS-----AVARFCPSNLVVIIQKVIAELIGTYFVIFGGC 74
           MATK +GI++EE S++E+G+  S      +   C +N+V ++QKVIAELIGTYFV+F GC
Sbjct: 1   MATKAEGIQEEEFSRMEDGVSSSPSRCDTLHNCCSNNVVTLVQKVIAELIGTYFVVFAGC 60

Query: 75  GAVVVNKIYGSVTFPG 134
           G+V VNKIYGSVTFPG
Sbjct: 61  GSVAVNKIYGSVTFPG 76

Query: 135  194
           
Sbjct: 121  76

Query: 195  228
           
Sbjct: 181  76

[Close]

BLAST of CU165969 vs. TrEMBL
Match: K7KAK5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G246700 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/77 (57.14%), Postives = 58/77 (75.32%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCP------SNLVVIIQKVIAELIGTYFVIFGG 74
           MA K +GI++EE+S +EEG+   + +R C       SN+V + QKV+AE+IGTYFV+F G
Sbjct: 1   MAAKSEGIQEEEMSSMEEGVSSPSSSRTCSMSHNCCSNVVTLAQKVMAEVIGTYFVVFAG 60

Query: 75  CGAVVVNKIYGSVTFPG 134
           CG+V VNKIYGSVTFPG
Sbjct: 61  CGSVAVNKIYGSVTFPG 77

Query: 135  194
           
Sbjct: 121  77

Query: 195  228
           
Sbjct: 181  77

[Close]

BLAST of CU165969 vs. TrEMBL
Match: A0A0B2S9W2_GLYSO (Putative aquaporin NIP-type OS=Glycine soja GN=glysoja_013426 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/77 (57.14%), Postives = 58/77 (75.32%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCP------SNLVVIIQKVIAELIGTYFVIFGG 74
           MA K +GI++EE+S +EEG+   + +R C       SN+V + QKV+AE+IGTYFV+F G
Sbjct: 1   MAAKSEGIQEEEMSSMEEGVSSPSSSRTCSMSHNCCSNVVTLAQKVMAEVIGTYFVVFAG 60

Query: 75  CGAVVVNKIYGSVTFPG 134
           CG+V VNKIYGSVTFPG
Sbjct: 61  CGSVAVNKIYGSVTFPG 77

Query: 135  194
           
Sbjct: 121  77

Query: 195  228
           
Sbjct: 181  77

[Close]

BLAST of CU165969 vs. TrEMBL
Match: A0A061EGH1_THECC (NOD26-like intrinsic protein 4,1 OS=Theobroma cacao GN=TCM_019213 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.1e-15
Identity = 48/78 (61.54%), Postives = 57/78 (73.08%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVS------AVARFCPSNLVVI-IQKVIAELIGTYFVIFG 74
           MATK D IE+EEISK+E+G   S       ++  C S  VV  +QK+IAELIGTYF+IF 
Sbjct: 1   MATKTDNIEEEEISKMEQGHPTSNKSETDQLSILCSSTSVVTTVQKLIAELIGTYFIIFA 60

Query: 75  GCGAVVVNKIYGSVTFPG 134
           GCG+V VNKIYGSVTFPG
Sbjct: 61  GCGSVAVNKIYGSVTFPG 78

Query: 135  194
           
Sbjct: 121  78

Query: 195  228
           
Sbjct: 181  78

[Close]

BLAST of CU165969 vs. NCBI nr
Match: gi|449464154|ref|XP_004149794.1| (PREDICTED: probable aquaporin NIP-type [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 1.6e-29
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 74
           MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV
Sbjct: 1   MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 60

Query: 75  NKIYGSVTFPG 134
           NKIYGSVTFPG
Sbjct: 61  NKIYGSVTFPG 71

Query: 135  194
           
Sbjct: 121  71

Query: 195  228
           
Sbjct: 181  71

[Close]

BLAST of CU165969 vs. NCBI nr
Match: gi|659114474|ref|XP_008457069.1| (PREDICTED: probable aquaporin NIP-type [Cucumis melo])

HSP 1 Score: 125.9 bits (315), Expect = 2.9e-26
Identity = 65/71 (91.55%), Postives = 66/71 (92.96%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCPSNLVVIIQKVIAELIGTYFVIFGGCGAVVV 74
           MATKIDGIEDEEISKLEEG VVSA+AR CPSN VVIIQKVIAELIGTYFVIF GCGAV V
Sbjct: 1   MATKIDGIEDEEISKLEEGTVVSAIARLCPSNSVVIIQKVIAELIGTYFVIFSGCGAVAV 60

Query: 75  NKIYGSVTFPG 134
           NKIYGSVTFPG
Sbjct: 61  NKIYGSVTFPG 71

Query: 135  194
           
Sbjct: 121  71

Query: 195  228
           
Sbjct: 181  71

[Close]

BLAST of CU165969 vs. NCBI nr
Match: gi|1012361856|gb|KYP73039.1| (putative aquaporin NIP-type [Cajanus cajan])

HSP 1 Score: 91.3 bits (225), Expect = 7.9e-16
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVS-----AVARFCPSNLVVIIQKVIAELIGTYFVIFGGC 74
           MATK +GI++EE S++E+G+  S      +   C +N+V ++QKVIAELIGTYFV+F GC
Sbjct: 1   MATKAEGIQEEEFSRMEDGVSSSPSRCDTLHNCCSNNVVTLVQKVIAELIGTYFVVFAGC 60

Query: 75  GAVVVNKIYGSVTFPG 134
           G+V VNKIYGSVTFPG
Sbjct: 61  GSVAVNKIYGSVTFPG 76

Query: 135  194
           
Sbjct: 121  76

Query: 195  228
           
Sbjct: 181  76

[Close]

BLAST of CU165969 vs. NCBI nr
Match: gi|356499099|ref|XP_003518381.1| (PREDICTED: probable aquaporin NIP-type [Glycine max])

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-14
Identity = 44/77 (57.14%), Postives = 58/77 (75.32%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVSAVARFCP------SNLVVIIQKVIAELIGTYFVIFGG 74
           MA K +GI++EE+S +EEG+   + +R C       SN+V + QKV+AE+IGTYFV+F G
Sbjct: 1   MAAKSEGIQEEEMSSMEEGVSSPSSSRTCSMSHNCCSNVVTLAQKVMAEVIGTYFVVFAG 60

Query: 75  CGAVVVNKIYGSVTFPG 134
           CG+V VNKIYGSVTFPG
Sbjct: 61  CGSVAVNKIYGSVTFPG 77

Query: 135  194
           
Sbjct: 121  77

Query: 195  228
           
Sbjct: 181  77

[Close]

BLAST of CU165969 vs. NCBI nr
Match: gi|590652058|ref|XP_007033055.1| (NOD26-like intrinsic protein 4,1 [Theobroma cacao])

HSP 1 Score: 85.1 bits (209), Expect = 5.6e-14
Identity = 48/78 (61.54%), Postives = 57/78 (73.08%), Query Frame = 3


Query: 15  MATKIDGIEDEEISKLEEGIVVS------AVARFCPSNLVVI-IQKVIAELIGTYFVIFG 74
           MATK D IE+EEISK+E+G   S       ++  C S  VV  +QK+IAELIGTYF+IF 
Sbjct: 1   MATKTDNIEEEEISKMEQGHPTSNKSETDQLSILCSSTSVVTTVQKLIAELIGTYFIIFA 60

Query: 75  GCGAVVVNKIYGSVTFPG 134
           GCG+V VNKIYGSVTFPG
Sbjct: 61  GCGSVAVNKIYGSVTFPG 78

Query: 135  194
           
Sbjct: 121  78

Query: 195  228
           
Sbjct: 181  78

The following BLAST results are available for this feature:

BLAST of CU165969 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NIP1_NICAL	9.3e-17	63.51	Probable aquaporin NIP-type OS=Nicotiana alata PE=2 SV=1	[more]
NIP41_ARATH	3.4e-11	47.44	Putative aquaporin NIP4-1 OS=Arabidopsis thaliana GN=NIP4-1 PE=3 SV=1	[more]
NIP42_ARATH	3.4e-11	48.05	Probable aquaporin NIP4-2 OS=Arabidopsis thaliana GN=NIP4-2 PE=2 SV=2	[more]
NIP11_ORYSJ	1.9e-06	61.54	Aquaporin NIP1-1 OS=Oryza sativa subsp. japonica GN=NIP1-1 PE=2 SV=1	[more]
NIP11_MAIZE	1.9e-06	47.46	Aquaporin NIP1-1 OS=Zea mays GN=NIP1-1 PE=2 SV=1	[more]

BLAST of CU165969 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA39_CUCSA	6.0e-31	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G345890 PE=3 SV=1	[more]
A0A151U148_CAJCA	2.9e-17	59.21	Putative aquaporin NIP-type OS=Cajanus cajan GN=KK1_005646 PE=3 SV=1	[more]
K7KAK5_SOYBN	5.5e-16	57.14	Uncharacterized protein OS=Glycine max GN=GLYMA_02G246700 PE=3 SV=1	[more]
A0A0B2S9W2_GLYSO	5.5e-16	57.14	Putative aquaporin NIP-type OS=Glycine soja GN=glysoja_013426 PE=3 SV=1	[more]
A0A061EGH1_THECC	2.1e-15	61.54	NOD26-like intrinsic protein 4,1 OS=Theobroma cacao GN=TCM_019213 PE=3 SV=1	[more]

BLAST of CU165969 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449464154\|ref\|XP_004149794.1\|	1.6e-29	100.00	PREDICTED: probable aquaporin NIP-type [Cucumis sativus]	[more]
gi\|659114474\|ref\|XP_008457069.1\|	2.9e-26	91.55	PREDICTED: probable aquaporin NIP-type [Cucumis melo]	[more]
gi\|1012361856\|gb\|KYP73039.1\|	7.9e-16	59.21	putative aquaporin NIP-type [Cajanus cajan]	[more]
gi\|356499099\|ref\|XP_003518381.1\|	1.5e-14	57.14	PREDICTED: probable aquaporin NIP-type [Glycine max]	[more]
gi\|590652058\|ref\|XP_007033055.1\|	5.6e-14	61.54	NOD26-like intrinsic protein 4,1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000425	MIP
IPR023271	Aquaporin-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0005215	transporter activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa3G345890	Csa3G345890	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
H0096979	H0096979	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000425	Major intrinsic protein	PANTHER	PTHR19139	AQUAPORIN TRANSPORTER	coord: 2..76 score: 1.6
IPR023271	Aquaporin-like	GENE3D	G3DSA:1.20.1080.10		coord: 41..69 score: 1.
IPR023271	Aquaporin-like	unknown	SSF81338	Aquaporin-like	coord: 40..68 score: 9.0
None	No IPR available	PANTHER	PTHR19139:SF181	AQUAPORIN NIP4-1-RELATED	coord: 2..76 score: 1.6