CU161234 (transcribed_cluster) Cucumber (Chinese Long) v2

BLAST of CU161234 vs. Swiss-Prot
Match: GH34_ARATH (Indole-3-acetic acid-amido synthetase GH3.4 OS=Arabidopsis thaliana GN=GH3.4 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 7.3e-12
Identity = 30/58 (51.72%), Postives = 43/58 (74.14%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           N AP F+F+RR+NV+L+I ++KTDEA L KAVE   R++   G ++++YT+YAD  TI
Sbjct: 423 NSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTI 480

Query: 61   120
           
Sbjct: 483  480

Query: 121  175
           
Sbjct: 543  480

HSP 2 Score: 33.1 bits (74), Expect = 1.7e+00
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = -1

Query: 177 AGLYRYCMGDIVRVTGF 228
           AGLYRY +GDI+RVTGF
Sbjct: 405 AGLYRYRVGDILRVTGF 421

BLAST of CU161234 vs. Swiss-Prot
Match: GH32_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.6e-11
Identity = 33/58 (56.90%), Postives = 41/58 (70.69%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           N AP FRFVRRKNV+L+I ++KTDEA L  AVE    ++  +GA IV+YT+ AD +TI
Sbjct: 437 NAAPQFRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTI 494

Query: 61   120
           
Sbjct: 497  494

Query: 121  175
           
Sbjct: 557  494

BLAST of CU161234 vs. Swiss-Prot
Match: GH32_ARATH (Indole-3-acetic acid-amido synthetase GH3.2 OS=Arabidopsis thaliana GN=GH3.2 PE=1 SV=3)

HSP 1 Score: 69.3 bits (168), Expect = 2.1e-11
Identity = 29/58 (50.00%), Postives = 42/58 (72.41%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           N AP F+F+RRKNV+L++ ++KTDEA L KAVE   R+    G ++++YT+YA+  TI
Sbjct: 429 NSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTI 486

Query: 61   120
           
Sbjct: 489  486

Query: 121  175
           
Sbjct: 549  486

HSP 2 Score: 33.1 bits (74), Expect = 1.7e+00
Identity = 14/17 (82.35%), Postives = 14/17 (82.35%), Query Frame = -1

Query: 177 AGLYRYCMGDIVRVTGF 228
           AGLYRY +GDI+RVTGF
Sbjct: 411 AGLYRYRVGDILRVTGF 427

BLAST of CU161234 vs. Swiss-Prot
Match: GH34_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.8e-11
Identity = 33/57 (57.89%), Postives = 40/57 (70.18%), Query Frame = -3

Query: 4   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLST 63
           N AP F FVRRKNV L+I ++KTDEA LH AV E  + +  FGA +V+YT+YAD +T
Sbjct: 434 NAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTAT 490

Query: 64   123
           
Sbjct: 494  490

Query: 124  175
           
Sbjct: 554  490

HSP 2 Score: 31.6 bits (70), Expect = 4.9e+00
Identity = 12/18 (66.67%), Postives = 14/18 (77.78%), Query Frame = -1

Query: 174 AGLYRYCMGDIVRVTGFK 228
           +GLYRY +GD++RV GFK
Sbjct: 416 SGLYRYRVGDVLRVAGFK 433

BLAST of CU161234 vs. Swiss-Prot
Match: GH31_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.0e-10
Identity = 31/58 (53.45%), Postives = 40/58 (68.97%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           NKAP F FVRR+NV L++ ++KTDE  LH AV    + +  FGA +V+YT+YAD +TI
Sbjct: 426 NKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATI 483

Query: 61   120
           
Sbjct: 486  483

Query: 121  175
           
Sbjct: 546  483

HSP 2 Score: 32.3 bits (72), Expect = 2.9e+00
Identity = 13/20 (65.00%), Postives = 15/20 (75.00%), Query Frame = -1

Query: 168 AGLYRYCMGDIVRVTGFKIK 227
           +GLYRY +GD++RV GFK K
Sbjct: 408 SGLYRYRVGDVLRVAGFKNK 427

BLAST of CU161234 vs. TrEMBL
Match: A0A0A0KAF9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.5e-24
Identity = 62/72 (86.11%), Postives = 64/72 (88.89%), Query Frame = -3

Query: 1   RHCSGDRI*-----NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 60
           R+C GD +      NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI
Sbjct: 405 RYCMGDIVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 464

Query: 61  VDYTTYADLSTI 120
           VDYTTYADLSTI
Sbjct: 465 VDYTTYADLSTI 476

Query: 121  180
           
Sbjct: 525  476

Query: 181  202
           
Sbjct: 585  476

BLAST of CU161234 vs. TrEMBL
Match: A0A0A0KAF9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1)

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-01
Identity = 19/20 (95.00%), Postives = 19/20 (95.00%), Query Frame = -1

Query: 168 AGLYRYCMGDIVRVTGFKIK 227
           AGLYRYCMGDIVRVTGFK K
Sbjct: 401 AGLYRYCMGDIVRVTGFKNK 420

HSP 2 Score: 79.0 bits (193), Expect = 3.0e-12
Identity = 39/58 (67.24%), Postives = 43/58 (74.14%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           NKAP F FVRRKNVVLN+  EKT+E  L   VE  G V+K FGA IVDYT+YAD STI
Sbjct: 420 NKAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTI 477

Query: 61   120
           
Sbjct: 480  477

Query: 121  175
           
Sbjct: 540  477

BLAST of CU161234 vs. TrEMBL
Match: A0A0A0LHA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G081190 PE=4 SV=1)

HSP 1 Score: 32.3 bits (72), Expect = 3.2e+02
Identity = 15/20 (75.00%), Postives = 16/20 (80.00%), Query Frame = -1

Query: 168 AGLYRYCMGDIVRVTGFKIK 227
           AGLYR  +GDIVRVTGF  K
Sbjct: 402 AGLYRCSLGDIVRVTGFTNK 421

HSP 2 Score: 76.3 bits (186), Expect = 1.9e-11
Identity = 34/58 (58.62%), Postives = 45/58 (77.59%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           NKAP FRFVRRKNV+L+I ++KTDEA LHKAVE    +++ F   +V+YT+YA+  TI
Sbjct: 424 NKAPQFRFVRRKNVLLSIESDKTDEAELHKAVENASLLLREFNTSVVEYTSYAETKTI 481

Query: 61   120
           
Sbjct: 484  481

Query: 121  175
           
Sbjct: 544  481

BLAST of CU161234 vs. TrEMBL
Match: A0A0D2SMB3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G234200 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-10
Identity = 33/58 (56.90%), Postives = 43/58 (74.14%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           N AP F FVRRKNV+L+I ++KTDEA L KAVE   R+++ F   +V+YT+YAD  TI
Sbjct: 425 NSAPQFHFVRRKNVLLSIDSDKTDEAELQKAVENASRLLREFNTSVVEYTSYADTKTI 482

Query: 61   120
           
Sbjct: 485  482

Query: 121  175
           
Sbjct: 545  482

BLAST of CU161234 vs. TrEMBL
Match: A0A0D2SMB3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G234200 PE=4 SV=1)

HSP 1 Score: 29.6 bits (65), Expect = 2.1e+03
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = -1

Query: 177 AGLYRYCMGDIVRVTGF 228
           AGL RY +GDI+RVTGF
Sbjct: 407 AGLCRYRVGDILRVTGF 423

HSP 2 Score: 73.2 bits (178), Expect = 1.6e-10
Identity = 33/58 (56.90%), Postives = 45/58 (77.59%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           N AP F FVRRKNV+L+I ++KTDEA L  AVE   ++++SF A +V+YT+YAD +TI
Sbjct: 424 NSAPQFHFVRRKNVLLSIDSDKTDEAELQLAVENASKLLRSFNASVVEYTSYADTTTI 481

Query: 61   120
           
Sbjct: 484  481

Query: 121  175
           
Sbjct: 544  481

BLAST of CU161234 vs. NCBI nr
Match: gi|449444564|ref|XP_004140044.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 7.5e-25
Identity = 62/72 (86.11%), Postives = 64/72 (88.89%), Query Frame = -3

Query: 1   RHCSGDRI*-----NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 60
           R+C GD +      NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI
Sbjct: 405 RYCMGDIVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 464

Query: 61  VDYTTYADLSTI 120
           VDYTTYADLSTI
Sbjct: 465 VDYTTYADLSTI 476

Query: 121  180
           
Sbjct: 525  476

Query: 181  202
           
Sbjct: 585  476

BLAST of CU161234 vs. NCBI nr
Match: gi|659094768|ref|XP_008448232.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo])

HSP 1 Score: 113.2 bits (282), Expect = 2.0e-22
Identity = 57/72 (79.17%), Postives = 61/72 (84.72%), Query Frame = -3

Query: 1   RHCSGDRI*-----NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 60
           R+C GD +      NKAP+F FVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMK FGA I
Sbjct: 404 RYCMGDVVRVTGFKNKAPNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATI 463

Query: 61  VDYTTYADLSTI 120
           VDYTTYAD+STI
Sbjct: 464 VDYTTYADISTI 475

Query: 121  180
           
Sbjct: 524  475

Query: 181  202
           
Sbjct: 584  475

BLAST of CU161234 vs. NCBI nr
Match: gi|659082377|ref|XP_008441807.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo])

HSP 1 Score: 81.6 bits (200), Expect = 6.6e-13
Identity = 42/72 (58.33%), Postives = 50/72 (69.44%), Query Frame = -3

Query: 1   RHCSGDRI*-----NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKI 60
           R+C GD +      NKAP F FVRRKNV+LN+  EKT+E+ L   VE+ G V+K FGA I
Sbjct: 407 RYCIGDILRVTGFTNKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATI 466

Query: 61  VDYTTYADLSTI 120
           VDYT YAD STI
Sbjct: 467 VDYTCYADTSTI 478

Query: 121  180
           
Sbjct: 527  478

Query: 181  202
           
Sbjct: 587  478

BLAST of CU161234 vs. NCBI nr
Match: gi|778668015|ref|XP_011649024.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X2 [Cucumis sativus])

HSP 1 Score: 79.7 bits (195), Expect = 2.5e-12
Identity = 39/58 (67.24%), Postives = 43/58 (74.14%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           NKAP F FVRRKNVVLN+  EKT+E  L   VE  G V+K FGA IVDYT+YAD STI
Sbjct: 406 NKAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTI 463

Query: 61   120
           
Sbjct: 466  463

Query: 121  175
           
Sbjct: 526  463

BLAST of CU161234 vs. NCBI nr
Match: gi|449442409|ref|XP_004138974.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X1 [Cucumis sativus])

HSP 1 Score: 79.7 bits (195), Expect = 2.5e-12
Identity = 39/58 (67.24%), Postives = 43/58 (74.14%), Query Frame = -3

Query: 1   NKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTI 60
           NKAP F FVRRKNVVLN+  EKT+E  L   VE  G V+K FGA IVDYT+YAD STI
Sbjct: 420 NKAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTI 477

Query: 61   120
           
Sbjct: 480  477

Query: 121  175
           
Sbjct: 540  477