CU144813 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU144813
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Sterol 3-beta-glucosyltransferase
Location	Chr1 : 26673394 .. 26686723 (-)
Sequence length	264

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CATTACAAATTGTGATGCTCTATTAGTTGGCACAAGAGGAGACGTTCAACCTTTTGTTTTCCATTGGGAAGCGTTTACAGGAGCATGGCCATAGAGTTAGATTGGCAACTCATGCAAATTTTAAAGATTTTGTACTCTCTACTGGACTGGAGTTCTTTCCTTTAGGAGGAGATGCTAAAGTTCTTGCCGACTATATGGTAAAGAATAAAGGATTCCTTCCATCAGGACCCTTCGGAGATACATGCCCAACGAAATCATTTGAAG

Library


Library category	ESTs
gynoecious flower library	1

Homology

[Close]

BLAST of CU144813 vs. Swiss-Prot
Match: U80A2_ARATH (Sterol 3-beta-glucosyltransferase UGT80A2 OS=Arabidopsis thaliana GN=UGT80A2 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 8.5e-22
Identity = 52/76 (68.42%), Postives = 58/76 (76.32%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           +QIVML  +          +I KRLQ++GHRVRLATHANFK+FVL+ GLEF+PLGGD KV
Sbjct: 193 MQIVMLI-VGTRGDVQPFVAIAKRLQDYGHRVRLATHANFKEFVLTAGLEFYPLGGDPKV 252

Query: 63  LADYMVKNKGFLPSGP 122
           LA YMVKNKGFLPSGP
Sbjct: 253 LAGYMVKNKGFLPSGP 267

Query: 123  182
           
Sbjct: 313  267

Query: 183  231
           
Sbjct: 373  267

[Close]

BLAST of CU144813 vs. Swiss-Prot
Match: U80B1_ARATH (Sterol 3-beta-glucosyltransferase UGT80B1 OS=Arabidopsis thaliana GN=UGT80B1 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-16
Identity = 38/57 (66.67%), Postives = 45/57 (78.95%), Query Frame = 3


Query: 60  SIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKVLADYMVKNKGFLPSGP 119
           ++ KRLQE GHRVRLATHANF+ FV + G+EF+PLGGD + LA YM +NKG +PSGP
Sbjct: 174 AMAKRLQEFGHRVRLATHANFRSFVRAAGVEFYPLGGDPRELAAYMARNKGLIPSGP 230

Query: 120  179
           
Sbjct: 234  230

Query: 180  231
           
Sbjct: 294  230

[Close]

BLAST of CU144813 vs. Swiss-Prot
Match: ATG26_YARLI (Sterol 3-beta-glucosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=ATG26 PE=3 SV=3)

HSP 1 Score: 52.0 bits (123), Expect = 3.8e-06
Identity = 26/57 (45.61%), Postives = 35/57 (61.40%), Query Frame = 3


Query: 60   SIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGD----AKVLADYMVKNKGFL 119
            S+GK L E GHRVR+ATH+ FKD++   G+EF  + GD     K++ D+ V +  FL
Sbjct: 1013 SLGKALIEEGHRVRIATHSEFKDWIEGYGIEFKEVAGDPSELMKIMVDHGVFSVSFL 1069

Query: 120   179
            
Sbjct: 1073  1069

Query: 180   219
            
Sbjct: 1133  1069

[Close]

BLAST of CU144813 vs. TrEMBL
Match: A0A166D3D7_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_005506 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.7e-21
Identity = 56/76 (73.68%), Postives = 62/76 (81.58%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIV+L  +          +IGKRLQ++GHRVRLATHANFKDFVLS+GLEFFPLGGD KV
Sbjct: 137 LQIVVLI-VGTRGDVQPFVAIGKRLQDYGHRVRLATHANFKDFVLSSGLEFFPLGGDPKV 196

Query: 63  LADYMVKNKGFLPSGP 122
           LA YMVKNKGFLPSGP
Sbjct: 197 LAGYMVKNKGFLPSGP 211

Query: 123  182
           
Sbjct: 257  211

Query: 183  231
           
Sbjct: 317  211

[Close]

BLAST of CU144813 vs. TrEMBL
Match: A0A0A0LXW5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G661230 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.3e-21
Identity = 51/51 (100.00%), Postives = 51/51 (100.00%), Query Frame = 3


Query: 78  QEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKVLADYMVKNKGFLPSGP 137
           QEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKVLADYMVKNKGFLPSGP
Sbjct: 6   QEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKVLADYMVKNKGFLPSGP 56

Query: 138  197
           
Sbjct: 66   56

Query: 198  231
           
Sbjct: 126  56

[Close]

BLAST of CU144813 vs. TrEMBL
Match: A0A0A0LXW5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G661230 PE=4 SV=1)

HSP 1 Score: 29.6 bits (65), Expect = 2.3e+03
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = 1


Query: 229 PSEIHAQRNHLK 265
           PSEIHAQRNHLK
Sbjct: 56  PSEIHAQRNHLK 67

HSP 2 Score: 109.0 bits (271), Expect = 2.9e-21
Identity = 55/76 (72.37%), Postives = 62/76 (81.58%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGKRLQ++GHRVRLATHANFK+FVLS+GLEFFPLGGD KV
Sbjct: 197 LQIVMLI-VGTRGDVQPFIAIGKRLQDYGHRVRLATHANFKEFVLSSGLEFFPLGGDPKV 256

Query: 63  LADYMVKNKGFLPSGP 122
           LA+YMVKNKGFLPS P
Sbjct: 257 LAEYMVKNKGFLPSAP 271

Query: 123  182
           
Sbjct: 317  271

Query: 183  231
           
Sbjct: 377  271

[Close]

BLAST of CU144813 vs. TrEMBL
Match: A0A059AXC4_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00810 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.9e-21
Identity = 55/76 (72.37%), Postives = 62/76 (81.58%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGKRLQ++GHRVRLATHANFK+FVL+ GLEFFPLGGD KV
Sbjct: 233 LQIVMLI-VGTRGDVQPFVAIGKRLQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPKV 292

Query: 63  LADYMVKNKGFLPSGP 122
           LA+YMVKNKGFLPSGP
Sbjct: 293 LANYMVKNKGFLPSGP 307

Query: 123  182
           
Sbjct: 353  307

Query: 183  231
           
Sbjct: 413  307

[Close]

BLAST of CU144813 vs. TrEMBL
Match: M5WHU1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019814mg PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.8e-21
Identity = 56/76 (73.68%), Postives = 60/76 (78.95%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGK LQE+GHRVRLATHANFKDFVL+ GLEFFPLGGD KV
Sbjct: 161 LQIVMLI-VGTRGDVQPFVAIGKSLQEYGHRVRLATHANFKDFVLTAGLEFFPLGGDPKV 220

Query: 63  LADYMVKNKGFLPSGP 122
           LA YMVKNKGFLPSGP
Sbjct: 221 LAGYMVKNKGFLPSGP 235

Query: 123  182
           
Sbjct: 281  235

Query: 183  231
           
Sbjct: 341  235

[Close]

BLAST of CU144813 vs. NCBI nr
Match: gi|778664181|ref|XP_011660238.1| (PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-25
Identity = 63/76 (82.89%), Postives = 64/76 (84.21%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          SIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV
Sbjct: 155 LQIVMLI-VGTRGDVQPFVSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 214

Query: 63  LADYMVKNKGFLPSGP 122
           LADYMVKNKGFLPSGP
Sbjct: 215 LADYMVKNKGFLPSGP 229

Query: 123  182
           
Sbjct: 275  229

Query: 183  231
           
Sbjct: 335  229

[Close]

BLAST of CU144813 vs. NCBI nr
Match: gi|778664184|ref|XP_011660239.1| (PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X2 [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-25
Identity = 63/76 (82.89%), Postives = 64/76 (84.21%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          SIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV
Sbjct: 74  LQIVMLI-VGTRGDVQPFVSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 133

Query: 63  LADYMVKNKGFLPSGP 122
           LADYMVKNKGFLPSGP
Sbjct: 134 LADYMVKNKGFLPSGP 148

Query: 123  182
           
Sbjct: 194  148

Query: 183  231
           
Sbjct: 254  148

[Close]

BLAST of CU144813 vs. NCBI nr
Match: gi|659086031|ref|XP_008443730.1| (PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X1 [Cucumis melo])

HSP 1 Score: 122.5 bits (306), Expect = 3.7e-25
Identity = 62/76 (81.58%), Postives = 64/76 (84.21%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV
Sbjct: 156 LQIVMLI-VGTRGDVQPFVAIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 215

Query: 63  LADYMVKNKGFLPSGP 122
           LADYMVKNKGFLPSGP
Sbjct: 216 LADYMVKNKGFLPSGP 230

Query: 123  182
           
Sbjct: 276  230

Query: 183  231
           
Sbjct: 336  230

[Close]

BLAST of CU144813 vs. NCBI nr
Match: gi|659086033|ref|XP_008443731.1| (PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X2 [Cucumis melo])

HSP 1 Score: 122.5 bits (306), Expect = 3.7e-25
Identity = 62/76 (81.58%), Postives = 64/76 (84.21%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV
Sbjct: 75  LQIVMLI-VGTRGDVQPFVAIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 134

Query: 63  LADYMVKNKGFLPSGP 122
           LADYMVKNKGFLPSGP
Sbjct: 135 LADYMVKNKGFLPSGP 149

Query: 123  182
           
Sbjct: 195  149

Query: 183  231
           
Sbjct: 255  149

[Close]

BLAST of CU144813 vs. NCBI nr
Match: gi|470138113|ref|XP_004304803.1| (PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 112.5 bits (280), Expect = 3.8e-22
Identity = 56/76 (73.68%), Postives = 62/76 (81.58%), Query Frame = 3


Query: 3   LQIVMLY*LAQEETFNLLFSIGKRLQEHGHRVRLATHANFKDFVLSTGLEFFPLGGDAKV 62
           LQIVML  +          +IGK+LQE+GHRVRLATHANFKDFVL+ GLEFFPLGGD KV
Sbjct: 158 LQIVMLI-VGTRGDVQPFVAIGKKLQEYGHRVRLATHANFKDFVLTAGLEFFPLGGDPKV 217

Query: 63  LADYMVKNKGFLPSGP 122
           LA+YMVKNKGFLPSGP
Sbjct: 218 LAEYMVKNKGFLPSGP 232

Query: 123  182
           
Sbjct: 278  232

Query: 183  231
           
Sbjct: 338  232

The following BLAST results are available for this feature:

BLAST of CU144813 vs. Swiss-Prot


Match Name	E-value	Identity	Description
U80A2_ARATH	8.5e-22	68.42	Sterol 3-beta-glucosyltransferase UGT80A2 OS=Arabidopsis thaliana GN=UGT80A2 PE=...	[more]
U80B1_ARATH	1.4e-16	66.67	Sterol 3-beta-glucosyltransferase UGT80B1 OS=Arabidopsis thaliana GN=UGT80B1 PE=...	[more]
ATG26_YARLI	3.8e-06	45.61	Sterol 3-beta-glucosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 15...	[more]

BLAST of CU144813 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A166D3D7_DAUCA	1.7e-21	73.68	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_005506 PE=4 SV=1	[more]
A0A0A0LXW5_CUCSA	2.3e-21	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G661230 PE=4 SV=1	[more]
A0A0A0LXW5_CUCSA	2.3e+03	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G661230 PE=4 SV=1	[more]
A0A059AXC4_EUCGR	2.9e-21	72.37	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00810 PE=4 SV=1	[more]
M5WHU1_PRUPE	3.8e-21	73.68	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019814mg PE=4 SV=1	[more]

BLAST of CU144813 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778664181\|ref\|XP_011660238.1\|	1.7e-25	82.89	PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X1 [Cucumis sa...	[more]
gi\|778664184\|ref\|XP_011660239.1\|	1.7e-25	82.89	PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X2 [Cucumis sa...	[more]
gi\|659086031\|ref\|XP_008443730.1\|	3.7e-25	81.58	PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X1 [Cucumis me...	[more]
gi\|659086033\|ref\|XP_008443731.1\|	3.7e-25	81.58	PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like isoform X2 [Cucumis me...	[more]
gi\|470138113\|ref\|XP_004304803.1\|	3.8e-22	73.68	PREDICTED: sterol 3-beta-glucosyltransferase UGT80A2-like [Fragaria vesca subsp....	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002213	UDP_glucos_trans
IPR004276	GlycoTrans_28_N

Vocabulary: **Molecular Function**
Term	Definition
GO:0016758	transferase activity, transferring hexosyl groups

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process
GO:0005975	carbohydrate metabolic process
GO:0030259	lipid glycosylation

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0030259	lipid glycosylation
biological_process	GO:0008152	metabolic process
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G661230	Csa1G661230	gene
Csa1G661240	Csa1G661240	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
G0122208	G0122208	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002213	UDP-glucuronosyl/UDP-glucosyltransferase	PANTHER	PTHR11926	GLUCOSYL/GLUCURONOSYL TRANSFERASES	coord: 22..77 score: 1.6
IPR004276	Glycosyltransferase family 28, N-terminal domain	PFAM	PF03033	Glyco_transf_28	coord: 20..66 score: 1.
None	No IPR available	GENE3D	G3DSA:3.40.50.2000		coord: 21..70 score: 2.5
None	No IPR available	PANTHER	PTHR11926:SF308	STEROL 3-BETA-GLUCOSYLTRANSFERASE UGT80A2	coord: 22..77 score: 1.6
None	No IPR available	unknown	SSF53756	UDP-Glycosyltransferase/glycogen phosphorylase	coord: 17..73 score: 1.88