CU133907 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU133907
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	DNA polymerase epsilon subunit 2
Location	Chr1 : 15969367 .. 15969805 (+)
Sequence length	217

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GGCGATGGTGTGCTACCTAAAGAGGAGACTCTCAGGCGTGCAGATCTGGAGAAGAAAGCAGTTAATGATATGTTTGTCATACTCTCTGACATTTGGTTGGATAGTGAAGAGGCCATGGGAAAACTGGAGACCATACTTGATGGTTTCGAGAATGTGGAAGTGGTTCCTTCCTTGTTTGTTTTGATGGGGAATTTTTGTTCCCATCCTTGTAATATTG

Library


Library category	ESTs
fruit (8DPP) library	1

Homology

[Close]

BLAST of CU133907 vs. Swiss-Prot
Match: DPB2_ARATH (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.2e-25
Identity = 53/72 (73.61%), Postives = 58/72 (80.56%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KEE ++ ADLE++AVND FVILSDIWLD EE M KLET+LDGFE+VE VPSLFV
Sbjct: 260 GGGTLTKEEMIKLADLERQAVNDTFVILSDIWLDDEEVMRKLETVLDGFESVETVPSLFV 319

Query: 61  LMGNFCSHPCNI 120
            MGNFCS PCN+
Sbjct: 320 FMGNFCSRPCNL 331

Query: 121  180
           
Sbjct: 380  331

Query: 181  217
           
Sbjct: 440  331

[Close]

BLAST of CU133907 vs. TrEMBL
Match: A0A0A0LX08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G435200 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.1e-33
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV
Sbjct: 34  GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 93

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCNI
Sbjct: 94  LMGNFCSHPCNI 105

Query: 121  180
           
Sbjct: 154  105

Query: 181  217
           
Sbjct: 214  105

[Close]

BLAST of CU133907 vs. TrEMBL
Match: A0A061FWB8_THECC (DNA polymerase epsilon subunit B2 isoform 1 OS=Theobroma cacao GN=TCM_013360 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-26
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KEETLR ADLEK+AVNDMFVILSDIWLD+EE M KLET+L+GFE+VE+VPSLFV
Sbjct: 262 GGGTLTKEETLRLADLEKRAVNDMFVILSDIWLDNEEVMEKLETVLNGFESVEIVPSLFV 321

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCN+
Sbjct: 322 LMGNFCSHPCNL 333

Query: 121  180
           
Sbjct: 382  333

Query: 181  217
           
Sbjct: 442  333

[Close]

BLAST of CU133907 vs. TrEMBL
Match: A0A061FXF2_THECC (DNA polymerase epsilon subunit B2 isoform 2 (Fragment) OS=Theobroma cacao GN=TCM_013360 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-26
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KEETLR ADLEK+AVNDMFVILSDIWLD+EE M KLET+L+GFE+VE+VPSLFV
Sbjct: 312 GGGTLTKEETLRLADLEKRAVNDMFVILSDIWLDNEEVMEKLETVLNGFESVEIVPSLFV 371

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCN+
Sbjct: 372 LMGNFCSHPCNL 383

Query: 121  180
           
Sbjct: 432  383

Query: 181  217
           
Sbjct: 492  383

[Close]

BLAST of CU133907 vs. TrEMBL
Match: A0A067DUC4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009713mg PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.9e-26
Identity = 58/72 (80.56%), Postives = 64/72 (88.89%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KE+TLR ADLEK+A NDMFVILSDIWLD+EE MGKLE +LDGFE+VEVVPSLFV
Sbjct: 262 GGGTLKKEDTLRLADLEKRAANDMFVILSDIWLDNEEVMGKLEVVLDGFESVEVVPSLFV 321

Query: 61  LMGNFCSHPCNI 120
            MGNFCSHPCN+
Sbjct: 322 FMGNFCSHPCNL 333

Query: 121  180
           
Sbjct: 382  333

Query: 181  217
           
Sbjct: 442  333

[Close]

BLAST of CU133907 vs. TrEMBL
Match: A0A067DVL2_CITSI (DNA polymerase epsilon subunit 2 OS=Citrus sinensis GN=CISIN_1g009713mg PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.9e-26
Identity = 58/72 (80.56%), Postives = 64/72 (88.89%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KE+TLR ADLEK+A NDMFVILSDIWLD+EE MGKLE +LDGFE+VEVVPSLFV
Sbjct: 262 GGGTLKKEDTLRLADLEKRAANDMFVILSDIWLDNEEVMGKLEVVLDGFESVEVVPSLFV 321

Query: 61  LMGNFCSHPCNI 120
            MGNFCSHPCN+
Sbjct: 322 FMGNFCSHPCNL 333

Query: 121  180
           
Sbjct: 382  333

Query: 181  217
           
Sbjct: 442  333

[Close]

BLAST of CU133907 vs. NCBI nr
Match: gi|778660923|ref|XP_011657177.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-33
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV
Sbjct: 262 GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 321

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCNI
Sbjct: 322 LMGNFCSHPCNI 333

Query: 121  180
           
Sbjct: 382  333

Query: 181  217
           
Sbjct: 442  333

[Close]

BLAST of CU133907 vs. NCBI nr
Match: gi|700210428|gb|KGN65524.1| (hypothetical protein Csa_1G435200 [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-33
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV
Sbjct: 34  GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 93

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCNI
Sbjct: 94  LMGNFCSHPCNI 105

Query: 121  180
           
Sbjct: 154  105

Query: 181  217
           
Sbjct: 214  105

[Close]

BLAST of CU133907 vs. NCBI nr
Match: gi|659066275|ref|XP_008463560.1| (PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo])

HSP 1 Score: 144.8 bits (364), Expect = 5.8e-32
Identity = 70/72 (97.22%), Postives = 70/72 (97.22%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G GVLPKEETLR ADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV
Sbjct: 263 GGGVLPKEETLRLADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 322

Query: 61  LMGNFCSHPCNI 120
           LMGNFCSHPCNI
Sbjct: 323 LMGNFCSHPCNI 334

Query: 121  180
           
Sbjct: 383  334

Query: 181  217
           
Sbjct: 443  334

[Close]

BLAST of CU133907 vs. NCBI nr
Match: gi|1012175509|ref|XP_015966828.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Arachis duranensis])

HSP 1 Score: 129.4 bits (324), Expect = 2.5e-27
Identity = 60/72 (83.33%), Postives = 66/72 (91.67%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KEET+R AD+EKKAVNDMFVILSDIWLD+EEAMGKLET+LDGFE+VEVVPSLFV
Sbjct: 265 GSGTLTKEETIRLADMEKKAVNDMFVILSDIWLDNEEAMGKLETVLDGFESVEVVPSLFV 324

Query: 61  LMGNFCSHPCNI 120
            MGNF SHPCN+
Sbjct: 325 FMGNFSSHPCNL 336

Query: 121  180
           
Sbjct: 385  336

Query: 181  217
           
Sbjct: 445  336

[Close]

BLAST of CU133907 vs. NCBI nr
Match: gi|1021519862|ref|XP_016203345.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Arachis ipaensis])

HSP 1 Score: 129.4 bits (324), Expect = 2.5e-27
Identity = 60/72 (83.33%), Postives = 66/72 (91.67%), Query Frame = 1


Query: 1   GDGVLPKEETLRRADLEKKAVNDMFVILSDIWLDSEEAMGKLETILDGFENVEVVPSLFV 60
           G G L KEET+R AD+EKKAVNDMFVILSDIWLD+EEAMGKLET+LDGFE+VEVVPSLFV
Sbjct: 265 GSGTLTKEETIRLADMEKKAVNDMFVILSDIWLDNEEAMGKLETVLDGFESVEVVPSLFV 324

Query: 61  LMGNFCSHPCNI 120
            MGNF SHPCN+
Sbjct: 325 FMGNFSSHPCNL 336

Query: 121  180
           
Sbjct: 385  336

Query: 181  217
           
Sbjct: 445  336

The following BLAST results are available for this feature:

BLAST of CU133907 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DPB2_ARATH	5.2e-25	73.61	DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1	[more]

BLAST of CU133907 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX08_CUCSA	2.1e-33	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G435200 PE=4 SV=1	[more]
A0A061FWB8_THECC	1.1e-26	81.94	DNA polymerase epsilon subunit B2 isoform 1 OS=Theobroma cacao GN=TCM_013360 PE=...	[more]
A0A061FXF2_THECC	1.1e-26	81.94	DNA polymerase epsilon subunit B2 isoform 2 (Fragment) OS=Theobroma cacao GN=TCM...	[more]
A0A067DUC4_CITSI	1.9e-26	80.56	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g009713mg PE=4 SV=1	[more]
A0A067DVL2_CITSI	1.9e-26	80.56	DNA polymerase epsilon subunit 2 OS=Citrus sinensis GN=CISIN_1g009713mg PE=3 SV=...	[more]

BLAST of CU133907 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778660923\|ref\|XP_011657177.1\|	1.4e-33	100.00	PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus]	[more]
gi\|700210428\|gb\|KGN65524.1\|	1.4e-33	100.00	hypothetical protein Csa_1G435200 [Cucumis sativus]	[more]
gi\|659066275\|ref\|XP_008463560.1\|	5.8e-32	97.22	PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo]	[more]
gi\|1012175509\|ref\|XP_015966828.1\|	2.5e-27	83.33	PREDICTED: DNA polymerase epsilon subunit 2 [Arachis duranensis]	[more]
gi\|1021519862\|ref\|XP_016203345.1\|	2.5e-27	83.33	PREDICTED: DNA polymerase epsilon subunit 2 [Arachis ipaensis]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR007185	DNA_pol_alpha/epsilon_bsu

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006260	DNA replication

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006260	DNA replication
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G435200	Csa1G435200	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02OU49L	FKNP3UI02OU49L	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007185	DNA polymerase alpha/epsilon, subunit B	PFAM	PF04042	DNA_pol_E_B	coord: 25..71 score: 1.
None	No IPR available	PANTHER	PTHR12708	DNA POLYMERASE EPSILON SUBUNIT B	coord: 1..71 score: 1.6
None	No IPR available	PANTHER	PTHR12708:SF0	DNA POLYMERASE EPSILON SUBUNIT 2	coord: 1..71 score: 1.6