CU130954 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU130954
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	rRNA N-glycosidase
Location	Chr5 : 15507680 .. 15507937 (+)
Sequence length	257

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CCAAAATTTGCTTTTGATATTCTCTTCCCCAAAACTAATCAAAATCTTCTCAACTTCGATAATTCCTTTAAATCCATTGAAATAGCAGCTAGTACTACAAGAGAAGCCACTCCCCTTGGTCTCAAACCATCGAATGCAGCCATTGCCAACTTGTTTCATTACGATCCTGTTCTGGCCCCTGTCTCGTTCCTCATTGTATTTCAAATGGTCTTCGAATCCGCCAAATTCAAATTTATCGAACAACGCATCGTTAATAG

Library


Library category	ESTs
fruit (8DPP) library	1

Homology

[Close]

BLAST of CU130954 vs. Swiss-Prot
Match: SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2)

HSP 1 Score: 100.1 bits (248), Expect = 1.2e-20
Identity = 47/83 (56.63%), Postives = 62/83 (74.70%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P+ AF  LF  T+QN+LNFDN+FKS+E AA TTR+   LG+ P + AI+NLF+ DP L P
Sbjct: 92  PQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQTIVLGVDPLDFAISNLFNADPKLLP 151

Query: 61  VSFLIVFQMVFESAKFKFIEQRI 120
           +SFL++ QMV E++KF+FIEQ +
Sbjct: 152 LSFLVIIQMVLEASKFRFIEQSV 174

Query: 121  180
           
Sbjct: 212  174

Query: 181  240
           
Sbjct: 272  174

Query: 241  250
           
Sbjct: 332  174

[Close]

BLAST of CU130954 vs. Swiss-Prot
Match: NIGB_SAMNI (Nigrin b OS=Sambucus nigra PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.3e-10
Identity = 34/76 (44.74%), Postives = 48/76 (63.16%), Query Frame = 1


Query: 22  LFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVF 81
           LF  T QN L+F  ++ ++E AA+T RE+  LG  P + AI +L+H D V    S L+V 
Sbjct: 126 LFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSLYHGDSVAR--SLLVVI 185

Query: 82  QMVFESAKFKFIEQRI 141
           QMV E+A+F++IEQ +
Sbjct: 186 QMVSEAARFRYIEQEV 199

Query: 142  201
           
Sbjct: 246  199

Query: 202  250
           
Sbjct: 306  199

[Close]

BLAST of CU130954 vs. Swiss-Prot
Match: RIP_LUFAC (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 8.1e-09
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 4   KFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAPV 63
           K A   +F  +    L +  +++ ++ AA   RE  PLG    ++AI  LFHYD   A  
Sbjct: 89  KLASQYVFAGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAA 148

Query: 64  SFLIVFQMVFESAKFKFIEQRIV 123
           +FL++ Q   ES++FK+IE +I+
Sbjct: 149 AFLVIIQTTAESSRFKYIEGQII 171

Query: 124  183
           
Sbjct: 209  171

Query: 184  243
           
Sbjct: 269  171

Query: 244  253
           
Sbjct: 329  171

[Close]

BLAST of CU130954 vs. Swiss-Prot
Match: RIPA_LUFAE (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-08
Identity = 28/83 (33.73%), Postives = 45/83 (54.22%), Query Frame = 1


Query: 4   KFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAPV 63
           K A   +F  +    L +  +++ ++ AA   RE  PLG    ++AI  LFHYD   A  
Sbjct: 109 KLASQYVFKGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAA 168

Query: 64  SFLIVFQMVFESAKFKFIEQRIV 123
           +FL++ Q   E+++FK+IE +I+
Sbjct: 169 AFLVIIQTTAEASRFKYIEGQII 191

Query: 124  183
           
Sbjct: 229  191

Query: 184  243
           
Sbjct: 289  191

Query: 244  253
           
Sbjct: 349  191

[Close]

BLAST of CU130954 vs. Swiss-Prot
Match: RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 4.0e-08
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P  A  +LF  T Q  L +  ++++++ AA   RE   LGL   ++AI  LFHY+   A 
Sbjct: 87  PAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLRENIELGLPALDSAITTLFHYNAEAAA 146

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
            + L++ Q   E+A+F++IE +I N
Sbjct: 147 SALLVLIQTTSEAARFRYIELQIAN 171

Query: 121  180
           
Sbjct: 207  171

Query: 181  240
           
Sbjct: 267  171

Query: 241  256
           
Sbjct: 327  171

[Close]

BLAST of CU130954 vs. TrEMBL
Match: A0A0A0KN71_CUCSA (rRNA N-glycosidase OS=Cucumis sativus GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 3.1e-39
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           PKFAFDILFPKTNQNLLNFDNSFKSIEIAA+TTREATPLGLKPSNAAIANLFHYDPVLAP
Sbjct: 102 PKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAP 161

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
           VSFLIVFQMVFESAKFKFIEQRIVN
Sbjct: 162 VSFLIVFQMVFESAKFKFIEQRIVN 186

Query: 121  180
           
Sbjct: 222  186

Query: 181  240
           
Sbjct: 282  186

Query: 241  256
           
Sbjct: 342  186

[Close]

BLAST of CU130954 vs. TrEMBL
Match: B7X8M2_MOMCH (rRNA N-glycosidase OS=Momordica charantia GN=MCL1 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-16
Identity = 43/85 (50.59%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P  A  ++F  TNQN++NF+N+F+SIEI   TTR  TPLG+    A+I +LF +D    P
Sbjct: 112 PPIASYVIFTDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVP 171

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
            SFL++ QMV E+AKFKFIEQ++++
Sbjct: 172 TSFLVLIQMVLEAAKFKFIEQKVIH 196

Query: 121  180
           
Sbjct: 232  196

Query: 181  240
           
Sbjct: 292  196

Query: 241  256
           
Sbjct: 352  196

[Close]

BLAST of CU130954 vs. TrEMBL
Match: A0A0A0K979_CUCSA (rRNA N-glycosidase OS=Cucumis sativus GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.8e-16
Identity = 44/84 (52.38%), Postives = 62/84 (73.81%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           PK A D++FP+T + +L F++ ++SIE A+ TTR  T LGL+P N+AI+NLFHY     P
Sbjct: 111 PKAALDVVFPETCRVVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIP 170

Query: 61  VSFLIVFQMVFESAKFKFIEQRIV 120
            SFL++ QMV E +KFKFIEQ ++
Sbjct: 171 GSFLVILQMVLEGSKFKFIEQSVI 194

Query: 121  180
           
Sbjct: 231  194

Query: 181  240
           
Sbjct: 291  194

Query: 241  253
           
Sbjct: 351  194

[Close]

BLAST of CU130954 vs. TrEMBL
Match: I6XQI9_BENHI (Ribosome-inactivating protein (Fragment) OS=Benincasa hispida GN=RIP PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.1e-07
Identity = 27/78 (34.62%), Postives = 51/78 (65.38%), Query Frame = 1


Query: 22  LFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVF 81
           +F  T    L + ++++ ++ AA   R++ PLG K  ++AI+ L++YD   AP +FL++ 
Sbjct: 30  VFQNTKMITLPYSSNYQKLQNAARKDRDSIPLGFKALDSAISTLYYYDAKAAPAAFLVLI 89

Query: 82  QMVFESAKFKFIEQRIVN 141
           Q   E+++FK+IEQ I++
Sbjct: 90  QSTAEASRFKYIEQLIID 107

Query: 142  201
           
Sbjct: 150  107

Query: 202  256
           
Sbjct: 210  107

[Close]

BLAST of CU130954 vs. TrEMBL
Match: I6YUR5_BENHI (Ribosome-inactivating protein (Fragment) OS=Benincasa hispida var. chieh-qua GN=RIP PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.1e-07
Identity = 27/78 (34.62%), Postives = 51/78 (65.38%), Query Frame = 1


Query: 22  LFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVF 81
           +F  T    L + ++++ ++ AA   R++ PLG K  ++AI+ L++YD   AP +FL++ 
Sbjct: 30  VFQNTKMITLPYSSNYQKLQNAARKDRDSIPLGFKALDSAISTLYYYDAKAAPAAFLVLI 89

Query: 82  QMVFESAKFKFIEQRIVN 141
           Q   E+++FK+IEQ I++
Sbjct: 90  QSTAEASRFKYIEQLIID 107

Query: 142  201
           
Sbjct: 150  107

Query: 202  256
           
Sbjct: 210  107

[Close]

BLAST of CU130954 vs. NCBI nr
Match: gi|449445192|ref|XP_004140357.1| (PREDICTED: nigrin b [Cucumis sativus])

HSP 1 Score: 166.8 bits (421), Expect = 1.7e-38
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           PKFAFDILFPKTNQNLLNFDNSFKSIEIAA+TTREATPLGLKPSNAAIANLFHYDPVLAP
Sbjct: 102 PKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAP 161

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
           VSFLIVFQMVFESAKFKFIEQRIVN
Sbjct: 162 VSFLIVFQMVFESAKFKFIEQRIVN 186

Query: 121  180
           
Sbjct: 222  186

Query: 181  240
           
Sbjct: 282  186

Query: 241  256
           
Sbjct: 342  186

[Close]

BLAST of CU130954 vs. NCBI nr
Match: gi|659126458|ref|XP_008463192.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 149.1 bits (375), Expect = 3.6e-33
Identity = 75/85 (88.24%), Postives = 79/85 (92.94%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           PKFAFDILFP TNQNLLNFD+SFKSIE AA+TTR+ATPLG K SNAAIANLFHYD VLAP
Sbjct: 102 PKFAFDILFPNTNQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAP 161

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
           VSFLIVFQM+FESAKF FIEQRIVN
Sbjct: 162 VSFLIVFQMIFESAKFNFIEQRIVN 186

Query: 121  180
           
Sbjct: 222  186

Query: 181  240
           
Sbjct: 282  186

Query: 241  256
           
Sbjct: 342  186

[Close]

BLAST of CU130954 vs. NCBI nr
Match: gi|529281106|pdb|4HR6|B (Chain B, Crystal Structure Of Snake Gourd (trichosanthes Anguina) Seed Lectin, A Three Chain Homologue Of Type Ii Rips)

HSP 1 Score: 97.8 bits (242), Expect = 9.5e-18
Identity = 47/83 (56.63%), Postives = 64/83 (77.11%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P+ AF  LF  T+QN+LNFDN+FKS+E AA TTR+   LG+ P + AI+NLF+ DP L P
Sbjct: 45  PQLAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQTIVLGVDPLDFAISNLFNADPKLLP 104

Query: 61  VSFLIVFQMVFESAKFKFIEQRI 120
           +SFL++ QMV E++KF+FIEQ +
Sbjct: 105 LSFLVIIQMVLEASKFRFIEQSV 127

Query: 121  180
           
Sbjct: 165  127

Query: 181  240
           
Sbjct: 225  127

Query: 241  250
           
Sbjct: 285  127

[Close]

BLAST of CU130954 vs. NCBI nr
Match: gi|659090016|ref|XP_008445788.1| (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 92.8 bits (229), Expect = 3.1e-16
Identity = 46/84 (54.76%), Postives = 63/84 (75.00%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P  AFDI+FPKT + LL F+++++SIE A+ TTR  T LGL+P N+AI+NLFHY     P
Sbjct: 110 PLVAFDIVFPKTCRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIP 169

Query: 61  VSFLIVFQMVFESAKFKFIEQRIV 120
            SFL++ QMV E +KFKFIE+ ++
Sbjct: 170 ESFLVILQMVLEGSKFKFIERSVI 193

Query: 121  180
           
Sbjct: 230  193

Query: 181  240
           
Sbjct: 290  193

Query: 241  253
           
Sbjct: 350  193

[Close]

BLAST of CU130954 vs. NCBI nr
Match: gi|1009083353|pdb|4Z8S|A (Chain A, Structural Studies On A Non-toxic Homologue Of Type Ii Rips From Momordica Charantia (bitter Gourd)-native-1)

HSP 1 Score: 90.9 bits (224), Expect = 1.2e-15
Identity = 43/85 (50.59%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   PKFAFDILFPKTNQNLLNFDNSFKSIEIAASTTREATPLGLKPSNAAIANLFHYDPVLAP 60
           P  A  ++F  TNQN++NF+N+F+SIEI   TTR  TPLG+    A+I +LF +D    P
Sbjct: 89  PPIASYVIFTDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVP 148

Query: 61  VSFLIVFQMVFESAKFKFIEQRIVN 120
            SFL++ QMV E+AKFKFIEQ++++
Sbjct: 149 TSFLVLIQMVLEAAKFKFIEQKVIH 173

Query: 121  180
           
Sbjct: 209  173

Query: 181  240
           
Sbjct: 269  173

Query: 241  256
           
Sbjct: 329  173

The following BLAST results are available for this feature:

BLAST of CU130954 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SGSL_TRIAN	1.2e-20	56.63	Seed lectin (Fragments) OS=Trichosanthes anguina PE=1 SV=2	[more]
NIGB_SAMNI	3.3e-10	44.74	Nigrin b OS=Sambucus nigra PE=1 SV=2	[more]
RIP_LUFAC	8.1e-09	34.94	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula PE=1 SV=2	[more]
RIPA_LUFAE	1.8e-08	33.73	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca PE=1 SV=1	[more]
RIP_CUCMO	4.0e-08	35.29	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata PE=1 SV=2	[more]

BLAST of CU130954 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN71_CUCSA	3.1e-39	98.82	rRNA N-glycosidase OS=Cucumis sativus GN=Csa_5G428440 PE=3 SV=1	[more]
B7X8M2_MOMCH	2.1e-16	50.59	rRNA N-glycosidase OS=Momordica charantia GN=MCL1 PE=2 SV=1	[more]
A0A0A0K979_CUCSA	4.8e-16	52.38	rRNA N-glycosidase OS=Cucumis sativus GN=Csa_6G040650 PE=3 SV=1	[more]
I6XQI9_BENHI	3.1e-07	34.62	Ribosome-inactivating protein (Fragment) OS=Benincasa hispida GN=RIP PE=4 SV=1	[more]
I6YUR5_BENHI	3.1e-07	34.62	Ribosome-inactivating protein (Fragment) OS=Benincasa hispida var. chieh-qua GN=...	[more]

BLAST of CU130954 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445192\|ref\|XP_004140357.1\|	1.7e-38	98.82	PREDICTED: nigrin b [Cucumis sativus]	[more]
gi\|659126458\|ref\|XP_008463192.1\|	3.6e-33	88.24	PREDICTED: nigrin b-like [Cucumis melo]	[more]
gi\|529281106\|pdb\|4HR6\|B	9.5e-18	56.63	Chain B, Crystal Structure Of Snake Gourd (trichosanthes Anguina) Seed Lectin, A...	[more]
gi\|659090016\|ref\|XP_008445788.1\|	3.1e-16	54.76	PREDICTED: nigrin b-like [Cucumis melo]	[more]
gi\|1009083353\|pdb\|4Z8S\|A	1.2e-15	50.59	Chain A, Structural Studies On A Non-toxic Homologue Of Type Ii Rips From Momord...	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001574	Ribosome_inactivat_prot
IPR016138	Ribosome_inactivat_prot_sub1
IPR017989	Ribosome_inactivat_1/2

Vocabulary: **Biological Process**
Term	Definition
GO:0017148	negative regulation of translation

Vocabulary: **Molecular Function**
Term	Definition
GO:0030598	rRNA N-glycosylase activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa5G428440	Csa5G428440	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02NJC1K	FKNP3UI02NJC1K	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 12..84 score: 8.
IPR001574	Ribosome-inactivating protein	unknown	SSF56371	Ribosome inactivating proteins (RIP)	coord: 3..84 score: 1.57
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	G3DSA:3.40.420.10		coord: 4..74 score: 3.2
IPR017989	Ribosome-inactivating protein subgroup	PRINTS	PR00396	SHIGARICIN	coord: 63..83 score: 1.3E-8coord: 34..48 score: 1.