CU129066 (transcribed_cluster) Cucumber (Chinese Long) v2

NameCU129066
Typetranscribed_cluster
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionMitogen-activated protein kinase
LocationChr5 : 82477 .. 85737 (-)
Sequence length219
The following sequences are available for this feature:

transcribed_cluster sequence

TGCAAATGCAGACTGCAAGCTGAAGATCTGTGACTTTGGACTTGCGCGTGCATCTTTCAGTGATGCCCCGTCTGCTATATTTTGGACTGATTATGTCGCTACTCGATGGTACCGTGCTCCTGAACTTTGTGGTTCTTTTTTCTCAAAGTATACACCAGCTATTGATATTTGGAGCATAGGATGTATATTTGCTGAAATGTTGGGAAGCAAGCCTTTGTT
Library categoryESTs
fruit (8DPP) library1
BLAST of CU129066 vs. Swiss-Prot
Match: MPK9_ARATH (Mitogen-activated protein kinase 9 OS=Arabidopsis thaliana GN=MPK9 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 3.3e-35
Identity = 67/72 (93.06%), Postives = 67/72 (93.06%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           AN+DCKLKICDFGLAR SF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 157 ANSDCKLKICDFGLARVSFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 216

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 217 CIFAEMLTGKPL 228

Query: 122 181
           
Sbjct: 277 228

Query: 182 218
           
Sbjct: 337 228

BLAST of CU129066 vs. Swiss-Prot
Match: MPK12_ORYSJ (Mitogen-activated protein kinase 12 OS=Oryza sativa subsp. japonica GN=MPK12 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 5.6e-35
Identity = 66/72 (91.67%), Postives = 68/72 (94.44%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           AN+DCKLKICDFGLARASF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 221 ANSDCKLKICDFGLARASFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 280

Query: 62  CIFAEMLGSKPL 121
           CIFAE+L  +PL
Sbjct: 281 CIFAELLTGRPL 292

Query: 122 181
           
Sbjct: 341 292

Query: 182 218
           
Sbjct: 401 292

BLAST of CU129066 vs. Swiss-Prot
Match: MPK8_ARATH (Mitogen-activated protein kinase 8 OS=Arabidopsis thaliana GN=MPK8 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 7.3e-35
Identity = 66/72 (91.67%), Postives = 67/72 (93.06%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLAR SF+DAP+AIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWS+G
Sbjct: 238 ANADCKLKICDFGLARVSFNDAPTAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSVG 297

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 298 CIFAEMLLGKPL 309

Query: 122 181
           
Sbjct: 358 309

Query: 182 218
           
Sbjct: 418 309

BLAST of CU129066 vs. Swiss-Prot
Match: MPK15_ARATH (Mitogen-activated protein kinase 15 OS=Arabidopsis thaliana GN=MPK15 PE=1 SV=3)

HSP 1 Score: 147.1 bits (370), Expect = 7.3e-35
Identity = 66/72 (91.67%), Postives = 67/72 (93.06%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLAR SF+DAP+AIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWS+G
Sbjct: 224 ANADCKLKICDFGLARVSFNDAPTAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSVG 283

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 284 CIFAEMLLGKPL 295

Query: 122 181
           
Sbjct: 344 295

Query: 182 218
           
Sbjct: 404 295

BLAST of CU129066 vs. Swiss-Prot
Match: MPK17_ORYSJ (Mitogen-activated protein kinase 17 OS=Oryza sativa subsp. japonica GN=MPK17 PE=2 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 1.6e-34
Identity = 64/72 (88.89%), Postives = 66/72 (91.67%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLK+CDFGLAR SF+D PSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWS+G
Sbjct: 239 ANADCKLKVCDFGLARVSFNDTPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSVG 298

Query: 62  CIFAEMLGSKPL 121
           CIFAE+L  KPL
Sbjct: 299 CIFAELLTGKPL 310

Query: 122 181
           
Sbjct: 359 310

Query: 182 218
           
Sbjct: 419 310

BLAST of CU129066 vs. TrEMBL
Match: A0A0A0KMF1_CUCSA (Mitogen-activated protein kinase OS=Cucumis sativus GN=Csa_5G002030 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.0e-35
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 150 ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 209

Query: 62  CIFAEMLGSKPL 121
           CIFAEMLGSKPL
Sbjct: 210 CIFAEMLGSKPL 221

Query: 122 181
           
Sbjct: 270 221

Query: 182 218
           
Sbjct: 330 221

BLAST of CU129066 vs. TrEMBL
Match: M0TWD2_MUSAM (Mitogen-activated protein kinase OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 5.6e-34
Identity = 69/72 (95.83%), Postives = 70/72 (97.22%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 230 ANADCKLKICDFGLARASFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 289

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 290 CIFAEMLTGKPL 301

Query: 122 181
           
Sbjct: 350 301

Query: 182 218
           
Sbjct: 410 301

BLAST of CU129066 vs. TrEMBL
Match: A0A0A0R603_MUSAC (Mitogen-activated protein kinase OS=Musa acuminata AAA Group GN=MAPK3 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 5.6e-34
Identity = 69/72 (95.83%), Postives = 70/72 (97.22%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 230 ANADCKLKICDFGLARASFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 289

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 290 CIFAEMLTGKPL 301

Query: 122 181
           
Sbjct: 350 301

Query: 182 218
           
Sbjct: 410 301

BLAST of CU129066 vs. TrEMBL
Match: A0A068TSP0_COFCA (Mitogen-activated protein kinase OS=Coffea canephora GN=GSCOC_T00022437001 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.5e-34
Identity = 68/72 (94.44%), Postives = 69/72 (95.83%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLAR SF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 236 ANADCKLKICDFGLARVSFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 295

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 296 CIFAEMLSGKPL 307

Query: 122 181
           
Sbjct: 356 307

Query: 182 218
           
Sbjct: 416 307

BLAST of CU129066 vs. TrEMBL
Match: A0A164SQ37_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023595 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.5e-34
Identity = 68/72 (94.44%), Postives = 69/72 (95.83%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASF DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPA+DIWSIG
Sbjct: 18  ANADCKLKICDFGLARASFDDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAVDIWSIG 77

Query: 62  CIFAEMLGSKPL 121
           CIFAEML  KPL
Sbjct: 78  CIFAEMLTGKPL 89

Query: 122 181
           
Sbjct: 138 89

Query: 182 218
           
Sbjct: 198 89

BLAST of CU129066 vs. NCBI nr
Match: gi|778697922|ref|XP_011654439.1| (PREDICTED: mitogen-activated protein kinase 17-like isoform X2 [Cucumis sativus])

HSP 1 Score: 158.3 bits (399), Expect = 5.0e-36
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 150 ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 209

Query: 62  CIFAEMLGSKPL 121
           CIFAEMLGSKPL
Sbjct: 210 CIFAEMLGSKPL 221

Query: 122 181
           
Sbjct: 270 221

Query: 182 218
           
Sbjct: 330 221

BLAST of CU129066 vs. NCBI nr
Match: gi|778697913|ref|XP_011654436.1| (PREDICTED: mitogen-activated protein kinase 9-like isoform X1 [Cucumis sativus])

HSP 1 Score: 158.3 bits (399), Expect = 5.0e-36
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 150 ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 209

Query: 62  CIFAEMLGSKPL 121
           CIFAEMLGSKPL
Sbjct: 210 CIFAEMLGSKPL 221

Query: 122 181
           
Sbjct: 270 221

Query: 182 218
           
Sbjct: 330 221

BLAST of CU129066 vs. NCBI nr
Match: gi|778697926|ref|XP_011654440.1| (PREDICTED: mitogen-activated protein kinase 12-like isoform X3 [Cucumis sativus])

HSP 1 Score: 158.3 bits (399), Expect = 5.0e-36
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 53  ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 112

Query: 62  CIFAEMLGSKPL 121
           CIFAEMLGSKPL
Sbjct: 113 CIFAEMLGSKPL 124

Query: 122 181
           
Sbjct: 173 124

Query: 182 218
           
Sbjct: 233 124

BLAST of CU129066 vs. NCBI nr
Match: gi|659109494|ref|XP_008454747.1| (PREDICTED: mitogen-activated protein kinase 17-like isoform X2 [Cucumis melo])

HSP 1 Score: 154.8 bits (390), Expect = 5.6e-35
Identity = 70/72 (97.22%), Postives = 71/72 (98.61%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 150 ANADCKLKICDFGLARASFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 209

Query: 62  CIFAEMLGSKPL 121
           CIFAEML SKPL
Sbjct: 210 CIFAEMLASKPL 221

Query: 122 181
           
Sbjct: 270 221

Query: 182 218
           
Sbjct: 330 221

BLAST of CU129066 vs. NCBI nr
Match: gi|659109484|ref|XP_008454742.1| (PREDICTED: mitogen-activated protein kinase 9-like isoform X1 [Cucumis melo])

HSP 1 Score: 154.8 bits (390), Expect = 5.6e-35
Identity = 70/72 (97.22%), Postives = 71/72 (98.61%), Query Frame = 2

Query: 2   ANADCKLKICDFGLARASFSDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 61
           ANADCKLKICDFGLARASF+DAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG
Sbjct: 150 ANADCKLKICDFGLARASFNDAPSAIFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIG 209

Query: 62  CIFAEMLGSKPL 121
           CIFAEML SKPL
Sbjct: 210 CIFAEMLASKPL 221

Query: 122 181
           
Sbjct: 270 221

Query: 182 218
           
Sbjct: 330 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MPK9_ARATH3.3e-3593.06Mitogen-activated protein kinase 9 OS=Arabidopsis thaliana GN=MPK9 PE=2 SV=2[more]
MPK12_ORYSJ5.6e-3591.67Mitogen-activated protein kinase 12 OS=Oryza sativa subsp. japonica GN=MPK12 PE=... [more]
MPK8_ARATH7.3e-3591.67Mitogen-activated protein kinase 8 OS=Arabidopsis thaliana GN=MPK8 PE=1 SV=2[more]
MPK15_ARATH7.3e-3591.67Mitogen-activated protein kinase 15 OS=Arabidopsis thaliana GN=MPK15 PE=1 SV=3[more]
MPK17_ORYSJ1.6e-3488.89Mitogen-activated protein kinase 17 OS=Oryza sativa subsp. japonica GN=MPK17 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KMF1_CUCSA1.0e-35100.00Mitogen-activated protein kinase OS=Cucumis sativus GN=Csa_5G002030 PE=4 SV=1[more]
M0TWD2_MUSAM5.6e-3495.83Mitogen-activated protein kinase OS=Musa acuminata subsp. malaccensis PE=4 SV=1[more]
A0A0A0R603_MUSAC5.6e-3495.83Mitogen-activated protein kinase OS=Musa acuminata AAA Group GN=MAPK3 PE=2 SV=1[more]
A0A068TSP0_COFCA9.5e-3494.44Mitogen-activated protein kinase OS=Coffea canephora GN=GSCOC_T00022437001 PE=4 ... [more]
A0A164SQ37_DAUCA9.5e-3494.44Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023595 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
gi|778697922|ref|XP_011654439.1|5.0e-36100.00PREDICTED: mitogen-activated protein kinase 17-like isoform X2 [Cucumis sativus][more]
gi|778697913|ref|XP_011654436.1|5.0e-36100.00PREDICTED: mitogen-activated protein kinase 9-like isoform X1 [Cucumis sativus][more]
gi|778697926|ref|XP_011654440.1|5.0e-36100.00PREDICTED: mitogen-activated protein kinase 12-like isoform X3 [Cucumis sativus][more]
gi|659109494|ref|XP_008454747.1|5.6e-3597.22PREDICTED: mitogen-activated protein kinase 17-like isoform X2 [Cucumis melo][more]
gi|659109484|ref|XP_008454742.1|5.6e-3597.22PREDICTED: mitogen-activated protein kinase 9-like isoform X1 [Cucumis melo][more]
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR011009Kinase-like_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Csa5G002030Csa5G002030gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
FKNP3UI02MS8PZFKNP3UI02MS8PZEST


Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..72
score: 1.6
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 1..74
score: 15
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 3..73
score: 5.63
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 3..72
score: 7.3
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 3..73
score: 6.6
NoneNo IPR availablePANTHERPTHR24055:SF211MITOGEN-ACTIVATED PROTEIN KINASE 16-RELATEDcoord: 3..73
score: 6.6