CU121195 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU121195
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Unknown protein
Location	Chr4 : 19948946 .. 19949279 (-)
Sequence length	249

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

AATTTCATTCATATTGGATTCCATGAGAATGCTTAATAGATCAGTTTGTATTGCTTCACCTGCTTTCATAGACTTTTCTCTTTCATCCATAATACCCAATATCAAAGTTGTTATCTCTTTGCTTATCTCTTTCATTTTTCTGTTTAACTTTGTGGGTAGAAATCTCCATCCAGGAATATATATCCCTAAGGTAGTTTGAATCACAAAATTAGTTAGTTCAGTTTGAAGCTTGAAGATCTTTTTTCCTTT

Library


Library category	ESTs
fruit (8DPP) library	2

Homology

[Close]

BLAST of CU121195 vs. Swiss-Prot
Match: C72A1_CATRO (Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.7e-17
Identity = 42/83 (50.60%), Postives = 59/83 (71.08%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G KIF+L  EL +  I     +YIPGW +LPTK N++MKEI+KEIT ++  I+++R K+
Sbjct: 224 EGGKIFRLLKELMDLTIDCMRDVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKA 283

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           +KAGE  + DLL +L+ESN+ EI
Sbjct: 284 LKAGEPGEDDLLGVLLESNIQEI 306

Query: 121  180
           
Sbjct: 344  306

Query: 181  240
           
Sbjct: 404  306

Query: 241  250
           
Sbjct: 464  306

[Close]

BLAST of CU121195 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.9e-16
Identity = 43/84 (51.19%), Postives = 59/84 (70.24%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G+KIF+LQ E    +IQ +  IY+PG RFLPTK N++MKEI+KE+   +  I+++R K+
Sbjct: 219 EGRKIFQLQREQAELIIQASQTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKA 278

Query: 61  MKAGE-AIQTDLLSILMESNMNEI 120
           M+AGE +   DLL IL+ESN  EI
Sbjct: 279 MEAGERSSHDDLLGILLESNSKEI 302

Query: 121  180
           
Sbjct: 339  302

Query: 181  240
           
Sbjct: 399  302

Query: 241  250
           
Sbjct: 459  302

[Close]

BLAST of CU121195 vs. Swiss-Prot
Match: C7A11_ARATH (Cytochrome P450 72A11 OS=Arabidopsis thaliana GN=CYP72A11 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 4.3e-15
Identity = 38/82 (46.34%), Postives = 58/82 (70.73%), Query Frame = -1


Query: 4   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 63
           +G++IF+LQ EL   +IQT    +IPG+ +LPTK NR+MK  ++EI  ++ GI+++R ++
Sbjct: 220 EGQRIFELQAELAQLIIQTVRKAFIPGYSYLPTKGNRRMKAKAREIQVILRGIVNKRLRA 279

Query: 64  MKAGEAIQTDLLSILMESNMNE 123
            +AGEA   DLL IL+ESN+ +
Sbjct: 280 REAGEAPNDDLLGILLESNLGQ 301

Query: 124  183
           
Sbjct: 340  301

Query: 184  243
           
Sbjct: 400  301

Query: 244  250
           
Sbjct: 460  301

[Close]

BLAST of CU121195 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 9.6e-15
Identity = 37/82 (45.12%), Postives = 57/82 (69.51%), Query Frame = -1


Query: 4   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 63
           +G++IF+LQ EL   +IQ     +IPG+ +LPTK NR+MK  ++EI  ++ GI+++R ++
Sbjct: 220 EGQRIFELQAELAQLIIQAFRKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRA 279

Query: 64  MKAGEAIQTDLLSILMESNMNE 123
            +AGEA   DLL IL+ESN+ +
Sbjct: 280 REAGEAPSDDLLGILLESNLRQ 301

Query: 124  183
           
Sbjct: 340  301

Query: 184  243
           
Sbjct: 400  301

Query: 244  250
           
Sbjct: 460  301

[Close]

BLAST of CU121195 vs. Swiss-Prot
Match: C7A14_ARATH (Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 2.1e-14
Identity = 37/82 (45.12%), Postives = 57/82 (69.51%), Query Frame = -1


Query: 4   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 63
           +G +IF+LQ EL   V+Q     +IPG+ +LPTK NR+MK  ++EI  ++ GI+++RE++
Sbjct: 220 EGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAREIQDILRGIINKRERA 279

Query: 64  MKAGEAIQTDLLSILMESNMNE 123
            ++GEA   DLL IL+ESN+ +
Sbjct: 280 RESGEAPSEDLLGILLESNLGQ 301

Query: 124  183
           
Sbjct: 340  301

Query: 184  243
           
Sbjct: 400  301

Query: 244  250
           
Sbjct: 460  301

[Close]

BLAST of CU121195 vs. TrEMBL
Match: F6I501_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02440 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.7e-22
Identity = 51/83 (61.45%), Postives = 67/83 (80.72%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G++IF+LQ E T+  IQ T+ +YIPGWRFLPTK NR+MK+ISKE+  L+ GI+++REK+
Sbjct: 110 EGRRIFQLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIINKREKA 169

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MKAGE   +DLL ILMESN  EI
Sbjct: 170 MKAGETANSDLLGILMESNFREI 192

Query: 121  180
           
Sbjct: 230  192

Query: 181  240
           
Sbjct: 290  192

Query: 241  250
           
Sbjct: 350  192

[Close]

BLAST of CU121195 vs. TrEMBL
Match: F6H5K6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0135g00290 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.7e-22
Identity = 51/83 (61.45%), Postives = 67/83 (80.72%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G++IF+LQ E T+  IQ T+ +YIPGWRFLPTK NR+MK+ISKE+  L+ GI+++REK+
Sbjct: 157 EGRRIFQLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIVNKREKA 216

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MKAGE   +DLL ILMESN  EI
Sbjct: 217 MKAGETANSDLLGILMESNFREI 239

Query: 121  180
           
Sbjct: 277  239

Query: 181  240
           
Sbjct: 337  239

Query: 241  250
           
Sbjct: 397  239

[Close]

BLAST of CU121195 vs. TrEMBL
Match: D7UAX7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02140 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 4.5e-19
Identity = 46/83 (55.42%), Postives = 64/83 (77.11%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G++IF+LQ E T   I+  + +YIPGWRF PTK N++MK+ISKE+  L+ GI+++REK+
Sbjct: 110 EGRRIFQLQKEQTYLAIKVAMSVYIPGWRFFPTKTNKRMKQISKEVHALLGGIINKREKA 169

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           M+AGE   +DLL ILMESN  EI
Sbjct: 170 MEAGETANSDLLGILMESNFREI 192

Query: 121  180
           
Sbjct: 230  192

Query: 181  240
           
Sbjct: 290  192

Query: 241  250
           
Sbjct: 350  192

[Close]

BLAST of CU121195 vs. TrEMBL
Match: F6I4Y3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02090 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.7e-18
Identity = 46/83 (55.42%), Postives = 63/83 (75.90%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G +IF+LQ E T   I+  + +YIPGWRF PTK N++MK+ISKE+  L+ GI+++REK+
Sbjct: 157 EGIRIFQLQKEQTYLAIKVAMSVYIPGWRFFPTKTNKRMKQISKEVHALLGGIINKREKA 216

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           M+AGE   +DLL ILMESN  EI
Sbjct: 217 MEAGETANSDLLGILMESNFREI 239

Query: 121  180
           
Sbjct: 277  239

Query: 181  240
           
Sbjct: 337  239

Query: 241  250
           
Sbjct: 397  239

[Close]

BLAST of CU121195 vs. TrEMBL
Match: M1BGG1_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017314 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-17
Identity = 45/83 (54.22%), Postives = 59/83 (71.08%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +G++IF+LQTE   + I+    +YIPGW FLPTK NR+MKEI + +   I GI+D+R K+
Sbjct: 222 EGRRIFELQTEQAQYFIEAVRSVYIPGWSFLPTKRNRRMKEIERHVQATIRGIIDKRVKA 281

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MK GEA   DLL IL+ESN  EI
Sbjct: 282 MKGGEANNEDLLGILLESNFKEI 304

Query: 121  180
           
Sbjct: 342  304

Query: 181  240
           
Sbjct: 402  304

Query: 241  250
           
Sbjct: 462  304

[Close]

BLAST of CU121195 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 163.3 bits (412), Expect = 1.8e-37
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS
Sbjct: 218 KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 277

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MKAGEAIQTDLLSILMESNMNEI
Sbjct: 278 MKAGEAIQTDLLSILMESNMNEI 300

Query: 121  180
           
Sbjct: 338  300

Query: 181  240
           
Sbjct: 398  300

Query: 241  250
           
Sbjct: 458  300

[Close]

BLAST of CU121195 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 146.7 bits (369), Expect = 1.7e-32
Identity = 74/83 (89.16%), Postives = 78/83 (93.98%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           KGKKIF+LQTEL N VIQ  LGIYIPGWRFLPTK NRKMKEISKEITTLILGIM+E+EKS
Sbjct: 223 KGKKIFELQTELANLVIQANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKS 282

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           +KAGEAIQTDLLSILMESNMNEI
Sbjct: 283 IKAGEAIQTDLLSILMESNMNEI 305

Query: 121  180
           
Sbjct: 343  305

Query: 181  240
           
Sbjct: 403  305

Query: 241  250
           
Sbjct: 463  305

[Close]

BLAST of CU121195 vs. NCBI nr
Match: gi|778697794|ref|XP_011654406.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 146.4 bits (368), Expect = 2.3e-32
Identity = 75/83 (90.36%), Postives = 77/83 (92.77%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           KGKKIFKLQTEL N VIQ +LGIYIPGW FLPTK NRKMKEISKEITTLILGIM+EREKS
Sbjct: 176 KGKKIFKLQTELGNLVIQASLGIYIPGWSFLPTKSNRKMKEISKEITTLILGIMNEREKS 235

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           M AGEAIQTDLLSILMESNMNEI
Sbjct: 236 MNAGEAIQTDLLSILMESNMNEI 258

Query: 121  180
           
Sbjct: 296  258

Query: 181  240
           
Sbjct: 356  258

Query: 241  250
           
Sbjct: 416  258

[Close]

BLAST of CU121195 vs. NCBI nr
Match: gi|778695577|ref|XP_004146006.2| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 2.4e-29
Identity = 67/83 (80.72%), Postives = 76/83 (91.57%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           +GKKIFKLQTELTN VIQ +L  YIPGWRFLPTK NRK+KE ++EITTL+LGI++E+EKS
Sbjct: 225 RGKKIFKLQTELTNLVIQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKS 284

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MKAGE IQTDLLSILMESNMNEI
Sbjct: 285 MKAGEEIQTDLLSILMESNMNEI 307

Query: 121  180
           
Sbjct: 345  307

Query: 181  240
           
Sbjct: 405  307

Query: 241  250
           
Sbjct: 465  307

[Close]

BLAST of CU121195 vs. NCBI nr
Match: gi|778695572|ref|XP_011654018.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X3 [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 2.4e-29
Identity = 67/83 (80.72%), Postives = 78/83 (93.98%), Query Frame = -1


Query: 1   KGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKS 60
           KGKKIF+LQ++L  F IQ++LGIYIPGWRFLPTK N KMKEISKEI+TLILG+M+E+EKS
Sbjct: 226 KGKKIFELQSKLGAFAIQSSLGIYIPGWRFLPTKSNNKMKEISKEISTLILGVMNEKEKS 285

Query: 61  MKAGEAIQTDLLSILMESNMNEI 120
           MKAGEAIQTDLLSIL+ESN+NEI
Sbjct: 286 MKAGEAIQTDLLSILIESNLNEI 308

Query: 121  180
           
Sbjct: 346  308

Query: 181  240
           
Sbjct: 406  308

Query: 241  250
           
Sbjct: 466  308

The following BLAST results are available for this feature:

BLAST of CU121195 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C72A1_CATRO	2.7e-17	50.60	Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1	[more]
C7A29_PANGI	3.9e-16	51.19	Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1	[more]
C7A11_ARATH	4.3e-15	46.34	Cytochrome P450 72A11 OS=Arabidopsis thaliana GN=CYP72A11 PE=2 SV=1	[more]
C7A15_ARATH	9.6e-15	45.12	Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1	[more]
C7A14_ARATH	2.1e-14	45.12	Cytochrome P450 72A14 OS=Arabidopsis thaliana GN=CYP72A14 PE=2 SV=1	[more]

BLAST of CU121195 vs. TrEMBL


Match Name	E-value	Identity	Description
F6I501_VITVI	5.7e-22	61.45	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02440 PE=3 SV=...	[more]
F6H5K6_VITVI	5.7e-22	61.45	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0135g00290 PE=3 SV=...	[more]
D7UAX7_VITVI	4.5e-19	55.42	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02140 PE=3 SV=...	[more]
F6I4Y3_VITVI	1.7e-18	55.42	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0015g02090 PE=3 SV=...	[more]
M1BGG1_SOLTU	1.1e-17	54.22	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017314 PE=3 SV=1	[more]

BLAST of CU121195 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778695562\|ref\|XP_011654016.1\|	1.8e-37	100.00	PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus]	[more]
gi\|659103032\|ref\|XP_008452438.1\|	1.7e-32	89.16	PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo]	[more]
gi\|778697794\|ref\|XP_011654406.1\|	2.3e-32	90.36	PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus]	[more]
gi\|778695577\|ref\|XP_004146006.2\|	2.4e-29	80.72	PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus]	[more]
gi\|778695572\|ref\|XP_011654018.1\|	2.4e-29	80.72	PREDICTED: cytochrome P450 CYP72A219 isoform X3 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa4G622230	Csa4G622230	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02MCQEA	FKNP3UI02MCQEA	EST
FKNP3UI02MTUV4	FKNP3UI02MTUV4	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 3..82 score: 9.5
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 4..79 score: 1.
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 4..81 score: 1.
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 2..83 score: 5.2
None	No IPR available	PANTHER	PTHR24282:SF16	SUBFAMILY NOT NAMED	coord: 2..83 score: 5.2