CU118235 (transcribed_cluster) Cucumber (Chinese Long) v2

Overview
NameCU118235
Typetranscribed_cluster
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionE3 ubiquitin-protein ligase
LocationChr4 : 10981036 .. 10981284 (-)
Sequence length249
Genome Viewer
Sequences
The following sequences are available for this feature:

transcribed_cluster sequence

CTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTT
Homology
[Close]
BLAST of CU118235 vs. Swiss-Prot
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.6e-16
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYSLE
Sbjct: 206 CFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 247

Query: 62   121
           
Sbjct: 266  247

Query: 122  128
           
Sbjct: 326  247


HSP 2 Score: 72.4 bits (176), Expect = 2.6e-12
Identity = 34/42 (80.95%), Postives = 35/42 (83.33%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL+
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALY 287

Query: 183  242
           
Sbjct: 306  287

Query: 243  249
           
Sbjct: 366  287

[Close]
BLAST of CU118235 vs. Swiss-Prot
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.6e-16
Identity = 37/42 (88.10%), Postives = 39/42 (92.86%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYSLE
Sbjct: 203 CFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLE 244

Query: 62   121
           
Sbjct: 263  244

Query: 122  128
           
Sbjct: 323  244


HSP 2 Score: 70.1 bits (170), Expect = 1.3e-11
Identity = 33/42 (78.57%), Postives = 34/42 (80.95%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VG + RKL WQGIPRSIRDSHRKVRDS DGLII R LAL+
Sbjct: 243 LEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALY 284

Query: 183  242
           
Sbjct: 303  284

Query: 243  249
           
Sbjct: 363  284

[Close]
BLAST of CU118235 vs. Swiss-Prot
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.2e-14
Identity = 35/42 (83.33%), Postives = 37/42 (88.10%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQR +ALF
Sbjct: 249 LEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALF 290

Query: 183  242
           
Sbjct: 309  290

Query: 243  249
           
Sbjct: 369  290


HSP 2 Score: 76.3 bits (186), Expect = 1.8e-13
Identity = 32/42 (76.19%), Postives = 37/42 (88.10%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFG+ FCLHFEAF+LG APVYMAFLRFMGD++EA+ ++YSLE
Sbjct: 209 CFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLE 250

Query: 62   121
           
Sbjct: 269  250

Query: 122  128
           
Sbjct: 329  250

[Close]
BLAST of CU118235 vs. Swiss-Prot
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 5.2e-13
Identity = 31/42 (73.81%), Postives = 36/42 (85.71%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFG+ FCLHFEAF+LG APVYMAFLRFMGD+ +A+ ++YSLE
Sbjct: 192 CFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLE 233

Query: 62   121
           
Sbjct: 252  233

Query: 122  128
           
Sbjct: 312  233


HSP 2 Score: 74.7 bits (182), Expect = 5.2e-13
Identity = 33/42 (78.57%), Postives = 36/42 (85.71%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +ALF
Sbjct: 232 LEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALF 273

Query: 183  242
           
Sbjct: 292  273

Query: 243  249
           
Sbjct: 352  273

[Close]
BLAST of CU118235 vs. Swiss-Prot
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 5.2e-13
Identity = 34/42 (80.95%), Postives = 37/42 (88.10%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +ALF
Sbjct: 250 LEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALF 291

Query: 183  242
           
Sbjct: 310  291

Query: 243  249
           
Sbjct: 370  291


HSP 2 Score: 74.3 bits (181), Expect = 6.8e-13
Identity = 31/42 (73.81%), Postives = 35/42 (83.33%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFG+ FCLHFEAF+LG  PVYMAFLRFMGD+ +A+ +SYSLE
Sbjct: 210 CFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYSLE 251

Query: 62   121
           
Sbjct: 270  251

Query: 122  128
           
Sbjct: 330  251

[Close]
BLAST of CU118235 vs. TrEMBL
Match: A0A0A0KWV9_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.5e-16
Identity = 42/42 (100.00%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE
Sbjct: 224 CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 265

Query: 62   121
           
Sbjct: 284  265

Query: 122  128
           
Sbjct: 344  265

[Close]
BLAST of CU118235 vs. TrEMBL
Match: A0A0A0KWV9_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-14
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF
Sbjct: 264 LEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 305

Query: 183  242
           
Sbjct: 324  305

Query: 243  249
           
Sbjct: 384  305


HSP 2 Score: 89.7 bits (221), Expect = 1.7e-15
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 2 

Query: 5   FGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLESW-WKWKKTHLARYSKKYS 64
           FGRQFCLHFEAF+LGTAPVYMAFLRFMGD++EAK+FSY LE+W  K KK  +  +S+K +
Sbjct: 138 FGRQFCLHFEAFQLGTAPVYMAFLRFMGDENEAKKFSYGLETWVIKKKKKKVGAHSRKLT 197

Query: 65  RQ 124
            Q
Sbjct: 198 WQ 199

Query: 125  184
           
Sbjct: 258  199

Query: 185  188
           
Sbjct: 318  199

[Close]
BLAST of CU118235 vs. TrEMBL
Match: A0A078FJP9_BRANA (E3 ubiquitin-protein ligase OS=Brassica napus GN=BnaC02g24630D PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 3.4e-03
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = 3 

Query: 126 KVGGNGRKLIWQGIPRSIRDSHRKVRD 185
           KVG + RKL WQGIPRSIRD HRKVR+
Sbjct: 188 KVGAHSRKLTWQGIPRSIRDGHRKVRE 214

Query: 186  207
           
Sbjct: 248  214


HSP 2 Score: 86.7 bits (213), Expect = 1.5e-14
Identity = 38/42 (90.48%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFG+QFCLHFEAF+LGTAPVYMAFLRFMGDD+EAK+FSYSLE
Sbjct: 205 CFGKQFCLHFEAFQLGTAPVYMAFLRFMGDDNEAKKFSYSLE 246

Query: 62   121
           
Sbjct: 265  246

Query: 122  128
           
Sbjct: 325  246

[Close]
BLAST of CU118235 vs. TrEMBL
Match: Q9XGC3_VITVI (E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_08s0007g06740 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 5.3e-12
Identity = 36/42 (85.71%), Postives = 39/42 (92.86%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+VGGN RKLIWQG+PRSIRDSHRKVRDS DGLIIQR LAL+
Sbjct: 245 LEVGGNSRKLIWQGVPRSIRDSHRKVRDSQDGLIIQRNLALY 286

Query: 183  242
           
Sbjct: 305  286

Query: 243  249
           
Sbjct: 365  286


HSP 2 Score: 86.7 bits (213), Expect = 1.5e-14
Identity = 38/42 (90.48%), Postives = 40/42 (95.24%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAF +GTAPVYMAFLRFMGDD EAKQF+YSLE
Sbjct: 206 CFGRQFCLHFEAFHIGTAPVYMAFLRFMGDDDEAKQFTYSLE 247

Query: 62   121
           
Sbjct: 266  247

Query: 122  128
           
Sbjct: 326  247

[Close]
BLAST of CU118235 vs. TrEMBL
Match: M5WJ19_PRUPE (E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa009039mg PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.4e-11
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 3 

Query: 123 LKVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALF 182
           L+V G+GRKLIWQGIPRSIRDSHRKVRDS DGLII R LALF
Sbjct: 246 LEVSGSGRKLIWQGIPRSIRDSHRKVRDSQDGLIIPRNLALF 287

Query: 183  242
           
Sbjct: 306  287

Query: 243  249
           
Sbjct: 366  287


HSP 2 Score: 86.3 bits (212), Expect = 1.9e-14
Identity = 38/42 (90.48%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAF+LGTAPVYMAFLRFMGD++EAK+FSYSLE
Sbjct: 205 CFGRQFCLHFEAFQLGTAPVYMAFLRFMGDENEAKKFSYSLE 246

Query: 62   121
           
Sbjct: 265  246

Query: 122  128
           
Sbjct: 325  246

[Close]
BLAST of CU118235 vs. NCBI nr
Match: gi|700198937|gb|KGN54095.1| (hypothetical protein Csa_4G285710 [Cucumis sativus])

HSP 1 Score: 92.0 bits (227), Expect = 5.0e-16
Identity = 42/42 (100.00%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE
Sbjct: 224 CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 265

Query: 62   121
           
Sbjct: 284  265

Query: 122  128
           
Sbjct: 344  265

[Close]
BLAST of CU118235 vs. NCBI nr
Match: gi|659097725|ref|XP_008449780.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-15
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDD+EAKQFSYSLE
Sbjct: 224 CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDNEAKQFSYSLE 265

Query: 62   121
           
Sbjct: 284  265

Query: 122  128
           
Sbjct: 344  265

[Close]
BLAST of CU118235 vs. NCBI nr
Match: gi|674918626|emb|CDY14650.1| (BnaC02g24630D [Brassica napus])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-14
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 2 

Query: 5   FGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLESW-WKWKKTHLARYSKKYS 64
           FGRQFCLHFEAF+LGTAPVYMAFLRFMGD++EAK+FSY LE+W  K KK  +  +S+K +
Sbjct: 138 FGRQFCLHFEAFQLGTAPVYMAFLRFMGDENEAKKFSYGLETWVIKKKKKKVGAHSRKLT 197

Query: 65  RQ 124
            Q
Sbjct: 198 WQ 199

Query: 125  184
           
Sbjct: 258  199

Query: 185  188
           
Sbjct: 318  199

[Close]
BLAST of CU118235 vs. NCBI nr
Match: gi|526117513|ref|NP_001268043.1| (SINA2p [Vitis vinifera])

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-14
Identity = 38/42 (90.48%), Postives = 42/42 (100.00%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFG+QFCLHFEAF+LGTAPVYMAFLRFMGDD+EAK+FSYSLE
Sbjct: 205 CFGKQFCLHFEAFQLGTAPVYMAFLRFMGDDNEAKKFSYSLE 246

Query: 62   121
           
Sbjct: 265  246

Query: 122  128
           
Sbjct: 325  246

[Close]
BLAST of CU118235 vs. NCBI nr
Match: gi|645215298|ref|XP_008226952.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2-like [Prunus mume])

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-14
Identity = 38/42 (90.48%), Postives = 40/42 (95.24%), Query Frame = 2 

Query: 2   CFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 61
           CFGRQFCLHFEAF +GTAPVYMAFLRFMGDD EAKQF+YSLE
Sbjct: 206 CFGRQFCLHFEAFHIGTAPVYMAFLRFMGDDDEAKQFNYSLE 247

Query: 62   121
           
Sbjct: 266  247

Query: 122  128
           
Sbjct: 326  247

The following BLAST results are available for this feature:
BLAST of CU118235 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SINA2_ARATH8.6e-1688.10E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1[more]
SINA1_ARATH8.6e-1688.10Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE... [more]
SINA3_ARATH6.2e-1483.33E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1[more]
SINA5_ARATH5.2e-1373.81E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2[more]
SINA4_ARATH5.2e-1380.95E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1[more]
BLAST of CU118235 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWV9_CUCSA3.5e-16100.00E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1[more]
A0A0A0KWV9_CUCSA1.1e-1497.62E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1[more]
A0A078FJP9_BRANA3.4e-0377.78E3 ubiquitin-protein ligase OS=Brassica napus GN=BnaC02g24630D PE=3 SV=1[more]
Q9XGC3_VITVI5.3e-1285.71E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_08s0007g06740 PE=2 SV=1[more]
M5WJ19_PRUPE3.4e-1185.71E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa009039mg PE=3 SV=1[more]
BLAST of CU118235 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700198937|gb|KGN54095.1|5.0e-16100.00hypothetical protein Csa_4G285710 [Cucumis sativus][more]
gi|659097725|ref|XP_008449780.1|1.1e-1597.62PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo][more]
gi|674918626|emb|CDY14650.1|1.2e-1469.35BnaC02g24630D [Brassica napus][more]
gi|526117513|ref|NP_001268043.1|2.1e-1490.48SINA2p [Vitis vinifera][more]
gi|645215298|ref|XP_008226952.1|2.1e-1490.48PREDICTED: E3 ubiquitin-protein ligase SINAT2-like [Prunus mume][more]
Annotated Terms
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR004162SINA-like
IPR008974TRAF-like
IPR0181217-in-absentia-prot_TRAF-dom
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Biological Process
TermDefinition
GO:0006511ubiquitin-dependent protein catabolic process
GO:0007275multicellular organism development
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Annotation
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
Relationships

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Csa4G285710Csa4G285710gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
FKNP3UI02LDUDCFKNP3UI02LDUDCEST
H0074730H0074730EST


InterPro
Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004162E3 ubiquitin-protein ligase SIN-likePANTHERPTHR10315SEVEN IN ABSENTIA HOMOLOGcoord: 2..42
score: 3.5
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 2..42
score: 8.9
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 2..43
score: 6.1
NoneNo IPR availablePANTHERPTHR10315:SF45E3 UBIQUITIN-PROTEIN LIGASEcoord: 2..42
score: 3.5