CU114994 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU114994
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Mitogen-activated protein kinase kinase 4
Location	Chr1 : 4621289 .. 4621540 (-)
Sequence length	259

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CGGCCGGGCTCCTCCGACCTCGACAAGCTCCAAGTCCTCGGTCACGGTAACGGCGGCACCGTCTACAAAGTCCGTCATAAACGCACCTCCGCTACTTACGCACTCAAGGTCGTCCACGGCGACTGCGACCCCACCGTTCGTCGCCAGGTTTTCAGAGAGATGGAGATTCTTCGTCGGACTGATTCTCCTTATGTTGTTCAGTGTCATGGAATCTTTGAGAAACCTTCTGGAGATGTAACGATTTTGATGGAGTATATGG

Library


Library category	ESTs
fruit (8DPP) library	3

Homology

[Close]

BLAST of CU114994 vs. Swiss-Prot
Match: M2K9_ARATH (Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana GN=MKK9 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 6.0e-28
Identity = 62/82 (75.61%), Postives = 66/82 (80.49%), Query Frame = 1


Query: 16  DLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYV 75
           DL+KL VLG GNGG VYKVRHK TS  YALK V+GD DP   RQ+ REMEILRRTDSPYV
Sbjct: 46  DLEKLNVLGCGNGGIVYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYV 105

Query: 76  VQCHGIFEKP-SGDVTILMEYM 135
           V+CHGIFEKP  G+V+ILMEYM
Sbjct: 106 VKCHGIFEKPVVGEVSILMEYM 127

Query: 136  195
           
Sbjct: 166  127

Query: 196  255
           
Sbjct: 226  127

Query: 256  259
           
Sbjct: 286  127

[Close]

BLAST of CU114994 vs. Swiss-Prot
Match: M2K7_ARATH (Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana GN=MKK7 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 3.1e-24
Identity = 56/84 (66.67%), Postives = 63/84 (75.00%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           +SD++KL VLG G+ G VYKV HK T   YALK V+GD  P   RQ+ REMEILRRTDSP
Sbjct: 42  ASDVEKLHVLGRGSSGIVYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSP 101

Query: 70  YVVQCHGIFEKP-SGDVTILMEYM 129
           YVV+C GIFEKP  G+V+ILMEYM
Sbjct: 102 YVVRCQGIFEKPIVGEVSILMEYM 125

Query: 130  189
           
Sbjct: 162  125

Query: 190  249
           
Sbjct: 222  125

Query: 250  259
           
Sbjct: 282  125

[Close]

BLAST of CU114994 vs. Swiss-Prot
Match: M2K8_ARATH (Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana GN=MKK8 PE=3 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 2.5e-18
Identity = 46/82 (56.10%), Postives = 58/82 (70.73%), Query Frame = 1


Query: 13  SDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 72
           ++LD++ VLG GNGGTV+KV+ K TS  YALK V  + D T      RE+EILR  +SPY
Sbjct: 51  ANLDRISVLGSGNGGTVFKVKDKTTSEIYALKKVKENWDSTS----LREIEILRMVNSPY 110

Query: 73  VVQCHGIFEKPSGDVTILMEYM 132
           V +CH IF+ PSG+V+ILM+YM
Sbjct: 111 VAKCHDIFQNPSGEVSILMDYM 128

Query: 133  192
           
Sbjct: 171  128

Query: 193  252
           
Sbjct: 231  128

Query: 253  259
           
Sbjct: 291  128

[Close]

BLAST of CU114994 vs. Swiss-Prot
Match: M2K5_ARATH (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana GN=MKK5 PE=1 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 6.2e-17
Identity = 41/82 (50.00%), Postives = 60/82 (73.17%), Query Frame = 1


Query: 13  SDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 72
           S+L+++  +G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P 
Sbjct: 68  SELERVNRIGSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPN 127

Query: 73  VVQCHGIFEKPSGDVTILMEYM 132
           VV+CH +F+  +G++ +L+E+M
Sbjct: 128 VVKCHDMFDH-NGEIQVLLEFM 148

Query: 133  192
           
Sbjct: 188  148

Query: 193  252
           
Sbjct: 248  148

Query: 253  259
           
Sbjct: 308  148

[Close]

BLAST of CU114994 vs. Swiss-Prot
Match: M2K4_ARATH (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana GN=MKK4 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 5.3e-16
Identity = 41/82 (50.00%), Postives = 60/82 (73.17%), Query Frame = 1


Query: 13  SDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 72
           SDL +   +G G GGTVYKV H+ +S  YALKV++G+ + TVRRQ+ RE+EILR  + P 
Sbjct: 77  SDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPN 136

Query: 73  VVQCHGIFEKPSGDVTILMEYM 132
           VV+CH +F++ +G++ +L+E+M
Sbjct: 137 VVKCHEMFDQ-NGEIQVLLEFM 157

Query: 133  192
           
Sbjct: 197  157

Query: 193  252
           
Sbjct: 257  157

Query: 253  259
           
Sbjct: 317  157

[Close]

BLAST of CU114994 vs. TrEMBL
Match: A0A0U5PS50_CUCSA (MAP kinase Kinase 4 OS=Cucumis sativus GN=MKK4 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 9.6e-41
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP
Sbjct: 45  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 104

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           YVVQCHGIFEKPSGDVTILMEYM
Sbjct: 105 YVVQCHGIFEKPSGDVTILMEYM 127

Query: 130  189
           
Sbjct: 165  127

Query: 190  249
           
Sbjct: 225  127

Query: 250  259
           
Sbjct: 285  127

[Close]

BLAST of CU114994 vs. TrEMBL
Match: A0A0A0LQY3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042980 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 2.8e-40
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           SSDLDKLQVLGHGNGGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSP
Sbjct: 45  SSDLDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSP 104

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           YVVQCHGIFEKPSGDVTILMEYM
Sbjct: 105 YVVQCHGIFEKPSGDVTILMEYM 127

Query: 130  189
           
Sbjct: 165  127

Query: 190  249
           
Sbjct: 225  127

Query: 250  259
           
Sbjct: 285  127

[Close]

BLAST of CU114994 vs. TrEMBL
Match: F6GSP7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g01970 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 3.0e-34
Identity = 68/83 (81.93%), Postives = 79/83 (95.18%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           ++DL+K++V+GHGNGGTVYKV+HKRT+A YALKVVHGDCDPTVRRQV REMEILR TDSP
Sbjct: 44  AADLEKIEVIGHGNGGTVYKVQHKRTAANYALKVVHGDCDPTVRRQVLREMEILRFTDSP 103

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           +VVQCHGIF+KPSGD+ ILMEYM
Sbjct: 104 FVVQCHGIFQKPSGDIAILMEYM 126

Query: 130  189
           
Sbjct: 164  126

Query: 190  249
           
Sbjct: 224  126

Query: 250  259
           
Sbjct: 284  126

[Close]

BLAST of CU114994 vs. TrEMBL
Match: A5AHG4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038954 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 3.0e-34
Identity = 68/83 (81.93%), Postives = 79/83 (95.18%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           ++DL+K++V+GHGNGGTVYKV+HKRT+A YALKVVHGDCDPTVRRQV REMEILR TDSP
Sbjct: 44  AADLEKIEVIGHGNGGTVYKVQHKRTAANYALKVVHGDCDPTVRRQVLREMEILRFTDSP 103

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           +VVQCHGIF+KPSGD+ ILMEYM
Sbjct: 104 FVVQCHGIFQKPSGDIAILMEYM 126

Query: 130  189
           
Sbjct: 164  126

Query: 190  249
           
Sbjct: 224  126

Query: 250  259
           
Sbjct: 284  126

[Close]

BLAST of CU114994 vs. TrEMBL
Match: V4VNQ3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021170mg PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 7.4e-33
Identity = 69/82 (84.15%), Postives = 75/82 (91.46%), Query Frame = 1


Query: 13  SDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 72
           SDL+KLQVLGHGNGGTVYKV+H+ T   YALKVVHGD DPTVRRQVFREMEILRRTDSP+
Sbjct: 51  SDLEKLQVLGHGNGGTVYKVQHRCTHKIYALKVVHGDADPTVRRQVFREMEILRRTDSPF 110

Query: 73  VVQCHGIFEKPSGDVTILMEYM 132
           +VQC GIFEKPSGD+ ILMEYM
Sbjct: 111 IVQCFGIFEKPSGDIAILMEYM 132

Query: 133  192
           
Sbjct: 171  132

Query: 193  252
           
Sbjct: 231  132

Query: 253  259
           
Sbjct: 291  132

[Close]

BLAST of CU114994 vs. NCBI nr
Match: gi|974569602|emb|CUX76643.1| (MAP kinase Kinase 4 [Cucumis sativus])

HSP 1 Score: 177.2 bits (448), Expect = 1.2e-41
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP
Sbjct: 45  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 104

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           YVVQCHGIFEKPSGDVTILMEYM
Sbjct: 105 YVVQCHGIFEKPSGDVTILMEYM 127

Query: 130  189
           
Sbjct: 165  127

Query: 190  249
           
Sbjct: 225  127

Query: 250  259
           
Sbjct: 285  127

[Close]

BLAST of CU114994 vs. NCBI nr
Match: gi|449439485|ref|XP_004137516.1| (PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis sativus])

HSP 1 Score: 175.6 bits (444), Expect = 3.6e-41
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           SSDLDKLQVLGHGNGGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSP
Sbjct: 45  SSDLDKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSP 104

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           YVVQCHGIFEKPSGDVTILMEYM
Sbjct: 105 YVVQCHGIFEKPSGDVTILMEYM 127

Query: 130  189
           
Sbjct: 165  127

Query: 190  249
           
Sbjct: 225  127

Query: 250  259
           
Sbjct: 285  127

[Close]

BLAST of CU114994 vs. NCBI nr
Match: gi|659066908|ref|XP_008466859.1| (PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo])

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-40
Identity = 81/83 (97.59%), Postives = 82/83 (98.80%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           SSDL+KLQVLGHGNGGTVYKVRHKRTS TYALKVVHGDCDPTVRRQVFREMEILRRTDSP
Sbjct: 45  SSDLEKLQVLGHGNGGTVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSP 104

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           YVVQCHGIFEKPSGDVTILMEYM
Sbjct: 105 YVVQCHGIFEKPSGDVTILMEYM 127

Query: 130  189
           
Sbjct: 165  127

Query: 190  249
           
Sbjct: 225  127

Query: 250  259
           
Sbjct: 285  127

[Close]

BLAST of CU114994 vs. NCBI nr
Match: gi|720048760|ref|XP_010271229.1| (PREDICTED: mitogen-activated protein kinase kinase 9-like [Nelumbo nucifera])

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-35
Identity = 70/82 (85.37%), Postives = 77/82 (93.90%), Query Frame = 1


Query: 13  SDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPY 72
           SDL+KL+VLGHGNGGTVYKVRHKRTSA YALKVV GDCD TVRRQ+FREMEILRRTDSPY
Sbjct: 51  SDLEKLRVLGHGNGGTVYKVRHKRTSAIYALKVVQGDCDTTVRRQIFREMEILRRTDSPY 110

Query: 73  VVQCHGIFEKPSGDVTILMEYM 132
           VV+CHG+FEKP+GD+  LMEYM
Sbjct: 111 VVRCHGVFEKPTGDIAFLMEYM 132

Query: 133  192
           
Sbjct: 171  132

Query: 193  252
           
Sbjct: 231  132

Query: 253  259
           
Sbjct: 291  132

[Close]

BLAST of CU114994 vs. NCBI nr
Match: gi|225456228|ref|XP_002283080.1| (PREDICTED: mitogen-activated protein kinase kinase 9 [Vitis vinifera])

HSP 1 Score: 155.6 bits (392), Expect = 3.9e-35
Identity = 68/83 (81.93%), Postives = 79/83 (95.18%), Query Frame = 1


Query: 10  SSDLDKLQVLGHGNGGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSP 69
           ++DL+K++V+GHGNGGTVYKV+HKRT+A YALKVVHGDCDPTVRRQV REMEILR TDSP
Sbjct: 44  AADLEKIEVIGHGNGGTVYKVQHKRTAANYALKVVHGDCDPTVRRQVLREMEILRFTDSP 103

Query: 70  YVVQCHGIFEKPSGDVTILMEYM 129
           +VVQCHGIF+KPSGD+ ILMEYM
Sbjct: 104 FVVQCHGIFQKPSGDIAILMEYM 126

Query: 130  189
           
Sbjct: 164  126

Query: 190  249
           
Sbjct: 224  126

Query: 250  259
           
Sbjct: 284  126

The following BLAST results are available for this feature:

BLAST of CU114994 vs. Swiss-Prot


Match Name	E-value	Identity	Description
M2K9_ARATH	6.0e-28	75.61	Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana GN=MKK9 PE=1 S...	[more]
M2K7_ARATH	3.1e-24	66.67	Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana GN=MKK7 PE=1 S...	[more]
M2K8_ARATH	2.5e-18	56.10	Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana GN=MKK8 PE=3 S...	[more]
M2K5_ARATH	6.2e-17	50.00	Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana GN=MKK5 PE=1 S...	[more]
M2K4_ARATH	5.3e-16	50.00	Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana GN=MKK4 PE=1 S...	[more]

BLAST of CU114994 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0U5PS50_CUCSA	9.6e-41	100.00	MAP kinase Kinase 4 OS=Cucumis sativus GN=MKK4 PE=3 SV=1	[more]
A0A0A0LQY3_CUCSA	2.8e-40	98.80	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G042980 PE=3 SV=1	[more]
F6GSP7_VITVI	3.0e-34	81.93	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g01970 PE=3 SV=...	[more]
A5AHG4_VITVI	3.0e-34	81.93	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038954 PE=3 SV=1	[more]
V4VNQ3_9ROSI	7.4e-33	84.15	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021170mg PE=3 SV=1	[more]

BLAST of CU114994 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|974569602\|emb\|CUX76643.1\|	1.2e-41	100.00	MAP kinase Kinase 4 [Cucumis sativus]	[more]
gi\|449439485\|ref\|XP_004137516.1\|	3.6e-41	98.80	PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis sativus]	[more]
gi\|659066908\|ref\|XP_008466859.1\|	1.1e-40	97.59	PREDICTED: mitogen-activated protein kinase kinase 9 [Cucumis melo]	[more]
gi\|720048760\|ref\|XP_010271229.1\|	1.7e-35	85.37	PREDICTED: mitogen-activated protein kinase kinase 9-like [Nelumbo nucifera]	[more]
gi\|225456228\|ref\|XP_002283080.1\|	3.9e-35	81.93	PREDICTED: mitogen-activated protein kinase kinase 9 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000719	Prot_kinase_dom
IPR011009	Kinase-like_dom_sf
IPR017441	Protein_kinase_ATP_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0004672	protein kinase activity
GO:0005524	ATP binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G042980	Csa1G042980	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02L9ETH	FKNP3UI02L9ETH	EST
FKNP3UI02N3YOL	FKNP3UI02N3YOL	EST
FKNP3UI02OIIES	FKNP3UI02OIIES	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 9..86 score: 1.9
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 7..87 score: 13
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 5..86 score: 3.32
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 13..36 scor
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 5..86 score: 2.0
None	No IPR available	PANTHER	PTHR24361	MITOGEN-ACTIVATED KINASE KINASE KINASE	coord: 5..86 score: 8.5
None	No IPR available	PANTHER	PTHR24361:SF344	MITOGEN-ACTIVATED PROTEIN KINASE KINASE 7-RELATED	coord: 5..86 score: 8.5