CU110349 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU110349
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	EF hand calcium-binding family protein
Location	Chr4 : 14429505 .. 14430058 (-)
Sequence length	554

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GACCTCCATTTTCTCTTCTCCTTCTCCCCAAGTTTCTCCTCTCTACCTTTCCCACTTTCTGCCATGATGAAAATCCCCAAAAGATTTAGCCCTAAACGCCTCTTCCGATCCAAAAAGCATCACTCCGCCGACGTATCCCGCTCGGATCCACCCTCTTTCGGCTCCGGTACCTCCTCTTCCTCGTCCTCTTCCGAGAATTTCTTCAAAGCTAACACCGTGAACACCGGTTACGGAACCCCGACTAGCGTTCTGGACTCGGACGGGAGGGGGAACGAGGGGGTTGTGTCGAGGAAGGAACTGGAGGCGATTCTTAGCCGGATCGCGTCAGAGGAAGAGGTTGCGATGATGTTGCGCGAAGTGGATAGCGGGGGCGATGGGGTTCATACGGTTGGAGGAATTGATGGCTCGGGTGGGGTCGGGTGAAGCGGCGGGAGAGTCGGAGATGCGGGAGACGTTTGAGTTTTTTGACGCGGATCAGGATGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTTAATCAATAGGAGATGAGCGGTGCACGTTAGAGTATTG

Library


Library category	ESTs
gynoecious flower library	5
hermaphrodite flower library	2
Cucumber male flower buds	1

Homology

[Close]

BLAST of CU110349 vs. Swiss-Prot
Match: CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-06
Identity = 30/49 (61.22%), Postives = 37/49 (75.51%), Query Frame = 2


Query: 377 GFIRLEELMARVGSGEAAGES-EMRETFEFFDADQDGRITAEELHGVFN 436
           G IRLEEL +RV S + A +S E++ETFEFFDAD+DG I+A+EL  VF+
Sbjct: 121 GTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFS 169

Query: 437  496
           
Sbjct: 181  169

Query: 497  521
           
Sbjct: 241  169

HSP 2 Score: 42.4 bits (98), Expect = 6.4e-03
Identity = 21/41 (51.22%), Postives = 28/41 (68.29%), Query Frame = 1


Query: 265 RGNEGVVSRKELEAILSRIA----SEEEVAMMLREVDSGGD 324
           R N+G VSR +LE++LSR+     +EEE+ +ML+EVD  GD
Sbjct: 80  RDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCDGD 120

Query: 325  376
           
Sbjct: 140  120

[Close]

BLAST of CU110349 vs. TrEMBL
Match: A0A0A0L0H1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.9e-38
Identity = 87/104 (83.65%), Postives = 87/104 (83.65%), Query Frame = 1


Query: 64  MMKIPKRFSPKRLFRSKKHHSADVSRXXXXXXXXXXXXXXXXXENFFKANTVNTGYGTPT 123
           MMKIPKRFSPKRLFRSKKHHSADVSR                 ENFFKANTVNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60

Query: 124 SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD 183
           SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD
Sbjct: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD 104

Query: 184  243
           
Sbjct: 121  104

Query: 244  303
           
Sbjct: 181  104

Query: 304  363
           
Sbjct: 241  104

Query: 364  376
           
Sbjct: 301  104

[Close]

BLAST of CU110349 vs. TrEMBL
Match: A0A0A0L0H1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-17
Identity = 48/51 (94.12%), Postives = 49/51 (96.08%), Query Frame = 2


Query: 365 AGAMGFIRLEELMARVGSGEAAGESEMRETFEFFDADQDGRITAEELHGVF 424
           +G  GFIRLEELMARVGSGEAAGESEMRETFEFFDADQDGRITAEELHGVF
Sbjct: 101 SGGDGFIRLEELMARVGSGEAAGESEMRETFEFFDADQDGRITAEELHGVF 151

Query: 425  484
           
Sbjct: 161  151

Query: 485  518
           
Sbjct: 221  151

HSP 2 Score: 62.8 bits (151), Expect = 5.1e-07
Identity = 31/50 (62.00%), Postives = 38/50 (76.00%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG---EAAGESEMRETFEFFDADQDGRITAEELHGVF 436
           G I +E LM+RVGS    E AG+SE+RE F+FFD D+DGRITA+ELH  F
Sbjct: 139 GCISVEALMSRVGSAAACEPAGDSELREAFDFFDTDRDGRITADELHSFF 188

Query: 437  496
           
Sbjct: 199  188

Query: 497  518
           
Sbjct: 259  188

[Close]

BLAST of CU110349 vs. TrEMBL
Match: W9RJF8_9ROSA (Putative calcium-binding protein CML36 OS=Morus notabilis GN=L484_015534 PE=4 SV=1)

HSP 1 Score: 43.1 bits (100), Expect = 4.2e-01
Identity = 24/42 (57.14%), Postives = 29/42 (69.05%), Query Frame = 1


Query: 265 RGNEGVVSRKELEAILSRIA-------SEEEVAMMLREVDSG 324
           R N+G+VSRKELEA+LSR+        SE+ VA ML EVD G
Sbjct: 94  RDNDGIVSRKELEALLSRLGGVGSISESEDFVASMLSEVDGG 135

Query: 325  370
           
Sbjct: 154  135

HSP 2 Score: 62.0 bits (149), Expect = 8.7e-07
Identity = 31/51 (60.78%), Postives = 40/51 (78.43%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG--EAAGESEMRETFEFFDADQDGRITAEELHGVFNQ 436
           G+I LE L++RVG+   E A E E++ETF+FFDAD DG+ITAEEL GVF +
Sbjct: 132 GYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTK 182

Query: 437  496
           
Sbjct: 192  182

Query: 497  524
           
Sbjct: 252  182

[Close]

BLAST of CU110349 vs. TrEMBL
Match: A0A067DGT9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-02
Identity = 47/134 (35.07%), Postives = 56/134 (41.79%), Query Frame = 1


Query: 67  MKIPKRFSPKRLFRSK-KHHSADVSRXXXXXXXXXXXXXXXXXENFFKANTVNTGYGTPT 126
           MK+ ++ SPKRLFRSK K     VSR                     K  +     GTPT
Sbjct: 1   MKLIEKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHN---KLGSAPDRVGTPT 60

Query: 127 SVLD------SD--------------------GRGNEGVVSRKELEAILSRIA----SEE 186
           SVL       SD                     R N+GVV R ELEA+L R+     ++E
Sbjct: 61  SVLHEISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQE 120

Query: 187 EVAMMLREVDSGGD 246
           EV  ML EVD  GD
Sbjct: 121 EVKSMLSEVDREGD 131

Query: 247  306
           
Sbjct: 181  131

Query: 307  366
           
Sbjct: 241  131

Query: 367  376
           
Sbjct: 301  131

HSP 2 Score: 62.0 bits (149), Expect = 8.7e-07
Identity = 31/51 (60.78%), Postives = 40/51 (78.43%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG--EAAGESEMRETFEFFDADQDGRITAEELHGVFNQ 436
           G+I LE L++RVG+   E A E E++ETF+FFDAD DG+ITAEEL GVF +
Sbjct: 132 GYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTK 182

Query: 437  496
           
Sbjct: 192  182

Query: 497  524
           
Sbjct: 252  182

[Close]

BLAST of CU110349 vs. TrEMBL
Match: V4SC13_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-02
Identity = 47/134 (35.07%), Postives = 56/134 (41.79%), Query Frame = 1


Query: 67  MKIPKRFSPKRLFRSK-KHHSADVSRXXXXXXXXXXXXXXXXXENFFKANTVNTGYGTPT 126
           MK+ ++ SPKRLFRSK K     VSR                     K  +     GTPT
Sbjct: 1   MKLIEKLSPKRLFRSKSKKDRTSVSRSDPSSFSSSSTSDSSTHN---KLGSAPDRVGTPT 60

Query: 127 SVLD------SD--------------------GRGNEGVVSRKELEAILSRIA----SEE 186
           SVL       SD                     R N+GVV R ELEA+L R+     ++E
Sbjct: 61  SVLHEISGDWSDMSADISLDMNYELVQACKLLDRDNDGVVLRSELEALLIRLGADPPTQE 120

Query: 187 EVAMMLREVDSGGD 246
           EV  ML EVD  GD
Sbjct: 121 EVKSMLSEVDREGD 131

Query: 247  306
           
Sbjct: 181  131

Query: 307  366
           
Sbjct: 241  131

Query: 367  376
           
Sbjct: 301  131

HSP 2 Score: 61.6 bits (148), Expect = 1.1e-06
Identity = 30/47 (63.83%), Postives = 38/47 (80.85%), Query Frame = 2


Query: 383 IRLEELMARVGS--GEAAGESEMRETFEFFDADQDGRITAEELHGVF 442
           + +EEL++RVG   G A  ++E+RETFEFFDAD DG+ITAEEL GVF
Sbjct: 137 VSIEELLSRVGPACGPACDDAELRETFEFFDADHDGKITAEELLGVF 183

Query: 443  502
           
Sbjct: 197  183

Query: 503  518
           
Sbjct: 257  183

[Close]

BLAST of CU110349 vs. NCBI nr
Match: gi|449467126|ref|XP_004151276.1| (PREDICTED: probable calcium-binding protein CML36 [Cucumis sativus])

HSP 1 Score: 164.1 bits (414), Expect = 2.3e-37
Identity = 87/104 (83.65%), Postives = 87/104 (83.65%), Query Frame = 1


Query: 64  MMKIPKRFSPKRLFRSKKHHSADVSRXXXXXXXXXXXXXXXXXENFFKANTVNTGYGTPT 123
           MMKIPKRFSPKRLFRSKKHHSADVSR                 ENFFKANTVNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60

Query: 124 SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD 183
           SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD
Sbjct: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD 104

Query: 184  243
           
Sbjct: 121  104

Query: 244  303
           
Sbjct: 181  104

Query: 304  363
           
Sbjct: 241  104

Query: 364  376
           
Sbjct: 301  104

[Close]

BLAST of CU110349 vs. NCBI nr
Match: gi|659115502|ref|XP_008457589.1| (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo])

HSP 1 Score: 147.5 bits (371), Expect = 2.3e-32
Identity = 80/104 (76.92%), Postives = 82/104 (78.85%), Query Frame = 1


Query: 64  MMKIPKRFSPKRLFRSKKHHSADVSRXXXXXXXXXXXXXXXXXENFFKANTVNTGYGTPT 123
           MMKIPKRFSPKRLFRSKKH SADVSR                 ENFFK N+VNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPT 60

Query: 124 SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGD 183
           SVLDSD R NEGVV+RKELEAILSRIASEEEVAMML EVDSGGD
Sbjct: 61  SVLDSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGD 104

Query: 184  243
           
Sbjct: 121  104

Query: 244  303
           
Sbjct: 181  104

Query: 304  363
           
Sbjct: 241  104

Query: 364  376
           
Sbjct: 301  104

[Close]

BLAST of CU110349 vs. NCBI nr
Match: gi|703123116|ref|XP_010102734.1| (putative calcium-binding protein CML36 [Morus notabilis])

HSP 1 Score: 62.4 bits (150), Expect = 9.6e-07
Identity = 31/50 (62.00%), Postives = 38/50 (76.00%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG---EAAGESEMRETFEFFDADQDGRITAEELHGVF 436
           G I +E LM+RVGS    E AG+SE+RE F+FFD D+DGRITA+ELH  F
Sbjct: 139 GCISVEALMSRVGSAAACEPAGDSELREAFDFFDTDRDGRITADELHSFF 188

Query: 437  496
           
Sbjct: 199  188

Query: 497  518
           
Sbjct: 259  188

[Close]

BLAST of CU110349 vs. NCBI nr
Match: gi|567857156|ref|XP_006421261.1| (hypothetical protein CICLE_v10005842mg [Citrus clementina])

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-06
Identity = 31/51 (60.78%), Postives = 40/51 (78.43%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG--EAAGESEMRETFEFFDADQDGRITAEELHGVFNQ 436
           G+I LE L++RVG+   E A E E++ETF+FFDAD DG+ITAEEL GVF +
Sbjct: 132 GYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTK 182

Query: 437  496
           
Sbjct: 192  182

Query: 497  524
           
Sbjct: 252  182

[Close]

BLAST of CU110349 vs. NCBI nr
Match: gi|568877049|ref|XP_006491566.1| (PREDICTED: probable calcium-binding protein CML36 [Citrus sinensis])

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-06
Identity = 31/51 (60.78%), Postives = 40/51 (78.43%), Query Frame = 2


Query: 377 GFIRLEELMARVGSG--EAAGESEMRETFEFFDADQDGRITAEELHGVFNQ 436
           G+I LE L++RVG+   E A E E++ETF+FFDAD DG+ITAEEL GVF +
Sbjct: 132 GYIPLEALISRVGNSSCEPACEPELKETFDFFDADHDGKITAEELFGVFTK 182

Query: 437  496
           
Sbjct: 192  182

Query: 497  524
           
Sbjct: 252  182

The following BLAST results are available for this feature:

BLAST of CU110349 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML36_ARATH	1.6e-06	61.22	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=...	[more]

BLAST of CU110349 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0H1_CUCSA	1.9e-38	83.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G355130 PE=4 SV=1	[more]
A0A0A0L0H1_CUCSA	1.4e-17	94.12	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G355130 PE=4 SV=1	[more]
W9RJF8_9ROSA	4.2e-01	57.14	Putative calcium-binding protein CML36 OS=Morus notabilis GN=L484_015534 PE=4 SV...	[more]
A0A067DGT9_CITSI	1.3e-02	35.07	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g027592mg PE=4 SV=1	[more]
V4SC13_9ROSI	1.3e-02	35.07	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005842mg PE=4 SV=1	[more]

BLAST of CU110349 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449467126\|ref\|XP_004151276.1\|	2.3e-37	83.65	PREDICTED: probable calcium-binding protein CML36 [Cucumis sativus]	[more]
gi\|659115502\|ref\|XP_008457589.1\|	2.3e-32	76.92	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo]	[more]
gi\|703123116\|ref\|XP_010102734.1\|	9.6e-07	62.00	putative calcium-binding protein CML36 [Morus notabilis]	[more]
gi\|567857156\|ref\|XP_006421261.1\|	1.3e-06	60.78	hypothetical protein CICLE_v10005842mg [Citrus clementina]	[more]
gi\|568877049\|ref\|XP_006491566.1\|	1.3e-06	60.78	PREDICTED: probable calcium-binding protein CML36 [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa4G355130	Csa4G355130	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
G0031839	G0031839	EST
G0082609	G0082609	EST
G0090669	G0090669	EST
G0174330	G0174330	EST
G0200768	G0200768	EST
H0050301	H0050301	EST
H0104885	H0104885	EST
csa02-2ms3-d08	csa02-2ms3-d08	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 148..168 score: 1.
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 93..126 score: 0.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 147..175 score: 0.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 93..110 score: 5.671coord: 143..178 score: 13
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 145..178 score: 2.9E-7coord: 83..126 score: 1.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 90..177 score: 2.2
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 156..168 scor