CU098916 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU098916
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	FAD/NAD(P)-binding oxidoreductase family protein
Location	Chr7 : 832766 .. 833378 (-)
Sequence length	612

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GCAATAACTTTTCTCGAAGTAACATTACCATTGCAGGCGACGCGCTCCACTCGATGACCCCAGATCTTGGCCAAGGTGGTTGTTCTGCATTGGAAGATGGTGTCATTCTCGCCCGTTGTTTAGGTGAAGCTATGTCGAGGAATCCAAATGGTGAAGTAGAAGACGAGGAGGAATATAAAAGAATAGAGAAAGGTTTGGAGAAATATGCAAAAGAGAGGAGATGGAGAAGCATTAAGCTTATAATTGCATCTGATGTGGTTGGCTCAATTCAAGAGAGTAAAGGGAAAGTGATGAACTACTTGAGAGATAATATCTTGGCTGATTCTCTTGTTGGTGTGTTAATGAAGATCTCTGATTTTGATTGTGGGACACTTAGTTGATGCATTAGCCCATTTTTTGTATGGTTTGGGTCGATGATATGTGGGCAAGAATTTTCTACTTTTTTTTGTCGATAATATATTACTTAATTATTTAAGTCATACATGAGTTGGCTAAATCAAAGTCGTTTTGTATTAATTTAAGACACTTTATGGTTTTTGTAATTTTTATTTTGGTTCATTCTGATATGTAGATTTTGGTACTATAATTTGGTTTTAATGATGTGTTATTCAT

Library


Library category	ESTs
fruit (8DPP) library	4
gynoecious flower library	1

Homology

[Close]

BLAST of CU098916 vs. Swiss-Prot
Match: HPXO_ACIAD (FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) GN=hpxO PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.4e-06
Identity = 27/79 (34.18%), Postives = 40/79 (50.63%), Query Frame = 3


Query: 9   FSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKRIE 68
           F +  + + GDA HS TPD+GQGGC A+ED + LAR L              +     +E
Sbjct: 275 FYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLARSL--------------QINTLSVE 334

Query: 69  KGLEKYAKERRWRSIKLII 128
             L +Y ++R  R+ +L++
Sbjct: 335 DALRRYQEKRNQRANELVL 339

Query: 129  188
           
Sbjct: 395  339

Query: 189  246
           
Sbjct: 455  339

[Close]

BLAST of CU098916 vs. TrEMBL
Match: A0A0A0K2K7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G014500 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.3e-63
Identity = 124/125 (99.20%), Postives = 125/125 (100.00%), Query Frame = 3


Query: 3   NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKR 62
           NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVED+EEYKR
Sbjct: 285 NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDKEEYKR 344

Query: 63  IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 122
           IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD
Sbjct: 345 IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 404

Query: 123 CGTLS 182
           CGTLS
Sbjct: 405 CGTLS 409

Query: 183  242
           
Sbjct: 465  409

Query: 243  302
           
Sbjct: 525  409

Query: 303  362
           
Sbjct: 585  409

Query: 363  378
           
Sbjct: 645  409

[Close]

BLAST of CU098916 vs. TrEMBL
Match: A0A072W2C4_MEDTR (FAD/NAD(P)-binding oxidoreductase family protein OS=Medicago truncatula GN=MTR_1g112780 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.5e-39
Identity = 83/123 (67.48%), Postives = 97/123 (78.86%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKRI 65
           N S+ N+ +AGDALH MTPDLGQGGCSALEDGV+LARCL EA S+ P  E E+EEEYKRI
Sbjct: 674 NISKGNVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAEAFSKKPKEEEEEEEEYKRI 733

Query: 66  EKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFDC 125
           E+G +KYAKERRWR I LIIA+  VGSIQES  K++N+ RD ILA  L   L+K S+FDC
Sbjct: 734 EEGFKKYAKERRWRCIDLIIANYFVGSIQESGSKLVNFFRDKILATYLASQLLKKSNFDC 793

Query: 126 GTL 185
           G L
Sbjct: 794 GKL 796

Query: 186  245
           
Sbjct: 854  796

Query: 246  305
           
Sbjct: 914  796

Query: 306  365
           
Sbjct: 974  796

Query: 366  375
           
Sbjct: 1034  796

[Close]

BLAST of CU098916 vs. TrEMBL
Match: A0A072W2C4_MEDTR (FAD/NAD(P)-binding oxidoreductase family protein OS=Medicago truncatula GN=MTR_1g112780 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.5e-33
Identity = 73/121 (60.33%), Postives = 90/121 (74.38%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKRI 65
           N S+ N+ +AGDALH MTPDLGQGGCSALEDGV+LARCL EA+S+      ++EE+YKRI
Sbjct: 285 NISKGNVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAEALSKKQ----KEEEKYKRI 344

Query: 66  EKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFDC 125
           E+GL+KYAKERRWR I LI  + + G IQ++  K + + RD ILA  L G L K S FDC
Sbjct: 345 EQGLKKYAKERRWRCIDLITTAYIAGVIQQADSKFVTFFRDKILAPLLAGQLFKKSGFDC 401

Query: 126 G 185
           G
Sbjct: 405 G 401

Query: 186  245
           
Sbjct: 465  401

Query: 246  305
           
Sbjct: 525  401

Query: 306  365
           
Sbjct: 585  401

Query: 366  369
           
Sbjct: 645  401

HSP 2 Score: 162.5 bits (410), Expect = 5.2e-37
Identity = 77/125 (61.60%), Postives = 99/125 (79.20%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPN--GEVEDEEEYK 65
           N S+ N+++AGDALH MTPD+GQGGC+ALEDGV+LARCLGEA+ +  N   E +++EEYK
Sbjct: 284 NISKGNVSVAGDALHPMTPDIGQGGCAALEDGVVLARCLGEALMKQVNVENEEKEKEEYK 343

Query: 66  RIEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDF 125
           RIE GL+KYA+ER WRS +LI  S +VG IQES GK++N+LRD I+   L G+ +K SDF
Sbjct: 344 RIEIGLKKYAQERGWRSFQLIATSYLVGFIQESDGKILNFLRDRIMVKYLAGLFLKRSDF 403

Query: 126 DCGTL 185
           DCG L
Sbjct: 404 DCGKL 408

Query: 186  245
           
Sbjct: 464  408

Query: 246  305
           
Sbjct: 524  408

Query: 306  365
           
Sbjct: 584  408

Query: 366  375
           
Sbjct: 644  408

[Close]

BLAST of CU098916 vs. TrEMBL
Match: U5GJH2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s18310g PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.2e-36
Identity = 75/126 (59.52%), Postives = 99/126 (78.57%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSR--NPNGEVEDEEEYK 65
           N S+ N+++AGDALH MTPD+GQGGC+ALEDGV+LARCL EA+ +  N  G+ ++ EEYK
Sbjct: 288 NISKGNVSVAGDALHPMTPDIGQGGCAALEDGVVLARCLAEALKKELNVEGKEKEREEYK 347

Query: 66  RIEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDF 125
           R+E GL+KYA ERRWRS +LI  + +VG+IQ+  GK+MN LRD  LA  L G+L+K +DF
Sbjct: 348 RVEMGLKKYAAERRWRSFELISTAYIVGAIQQGDGKIMNILRDAFLAKFLAGLLLKKADF 407

Query: 126 DCGTLS 185
           DCG L+
Sbjct: 408 DCGKLN 413

Query: 186  245
           
Sbjct: 468  413

Query: 246  305
           
Sbjct: 528  413

Query: 306  365
           
Sbjct: 588  413

Query: 366  378
           
Sbjct: 648  413

[Close]

BLAST of CU098916 vs. TrEMBL
Match: A0A067FL81_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g015061mg PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.0e-36
Identity = 75/125 (60.00%), Postives = 99/125 (79.20%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEY-KR 65
           N SR ++ +AGDALH MTPD+GQGGC+ALEDG++LARC+ EA+        EDEEE+ KR
Sbjct: 288 NISRGSVCVAGDALHPMTPDIGQGGCAALEDGIVLARCINEALKTKQGVGEEDEEEFNKR 347

Query: 66  IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 125
           +E GL++YAKERRWR  +LI  + +VGSIQ+S GK++N+LRD ILA  LVG+L+K +DFD
Sbjct: 348 VEMGLKRYAKERRWRCFELISIAYLVGSIQQSDGKILNFLRDKILASFLVGLLLKKADFD 407

Query: 126 CGTLS 185
           CG L+
Sbjct: 408 CGNLT 412

Query: 186  245
           
Sbjct: 468  412

Query: 246  305
           
Sbjct: 528  412

Query: 306  365
           
Sbjct: 588  412

Query: 366  378
           
Sbjct: 648  412

[Close]

BLAST of CU098916 vs. NCBI nr
Match: gi|449454223|ref|XP_004144855.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic [Cucumis sativus])

HSP 1 Score: 246.5 bits (628), Expect = 4.0e-62
Identity = 124/125 (99.20%), Postives = 125/125 (100.00%), Query Frame = 3


Query: 3   NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKR 62
           NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVED+EEYKR
Sbjct: 285 NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDKEEYKR 344

Query: 63  IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 122
           IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD
Sbjct: 345 IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 404

Query: 123 CGTLS 182
           CGTLS
Sbjct: 405 CGTLS 409

Query: 183  242
           
Sbjct: 465  409

Query: 243  302
           
Sbjct: 525  409

Query: 303  362
           
Sbjct: 585  409

Query: 363  378
           
Sbjct: 645  409

[Close]

BLAST of CU098916 vs. NCBI nr
Match: gi|659094208|ref|XP_008447940.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 235.7 bits (600), Expect = 7.0e-59
Identity = 118/124 (95.16%), Postives = 121/124 (97.58%), Query Frame = 3


Query: 3   NNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKR 62
           NNFSR N++IAGDALHSMTPDLGQGGCSALEDGVILARCL EAMSRNPNGEVED+EEYKR
Sbjct: 284 NNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILARCLAEAMSRNPNGEVEDKEEYKR 343

Query: 63  IEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 122
           IEKGLEKYAKERRWRSIKLIIAS VVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD
Sbjct: 344 IEKGLEKYAKERRWRSIKLIIASYVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFD 403

Query: 123 CGTL 182
           CGTL
Sbjct: 404 CGTL 407

Query: 183  242
           
Sbjct: 464  407

Query: 243  302
           
Sbjct: 524  407

Query: 303  362
           
Sbjct: 584  407

Query: 363  375
           
Sbjct: 644  407

[Close]

BLAST of CU098916 vs. NCBI nr
Match: gi|922408735|ref|XP_013470337.1| (FAD/NAD(P)-binding oxidoreductase family protein [Medicago truncatula])

HSP 1 Score: 169.1 bits (427), Expect = 8.0e-39
Identity = 83/123 (67.48%), Postives = 97/123 (78.86%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEEEYKRI 65
           N S+ N+ +AGDALH MTPDLGQGGCSALEDGV+LARCL EA S+ P  E E+EEEYKRI
Sbjct: 674 NISKGNVCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAEAFSKKPKEEEEEEEEYKRI 733

Query: 66  EKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDFDC 125
           E+G +KYAKERRWR I LIIA+  VGSIQES  K++N+ RD ILA  L   L+K S+FDC
Sbjct: 734 EEGFKKYAKERRWRCIDLIIANYFVGSIQESGSKLVNFFRDKILATYLASQLLKKSNFDC 793

Query: 126 GTL 185
           G L
Sbjct: 794 GKL 796

Query: 186  245
           
Sbjct: 854  796

Query: 246  305
           
Sbjct: 914  796

Query: 306  365
           
Sbjct: 974  796

Query: 366  375
           
Sbjct: 1034  796

[Close]

BLAST of CU098916 vs. NCBI nr
Match: gi|802675046|ref|XP_012081816.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Jatropha curcas])

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-36
Identity = 77/125 (61.60%), Postives = 99/125 (79.20%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPN--GEVEDEEEYK 65
           N S+ N+++AGDALH MTPD+GQGGC+ALEDGV+LARCLGEA+ +  N   E +++EEYK
Sbjct: 284 NISKGNVSVAGDALHPMTPDIGQGGCAALEDGVVLARCLGEALMKQVNVENEEKEKEEYK 343

Query: 66  RIEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMKISDF 125
           RIE GL+KYA+ER WRS +LI  S +VG IQES GK++N+LRD I+   L G+ +K SDF
Sbjct: 344 RIEIGLKKYAQERGWRSFQLIATSYLVGFIQESDGKILNFLRDRIMVKYLAGLFLKRSDF 403

Query: 126 DCGTL 185
           DCG L
Sbjct: 404 DCGKL 408

Query: 186  245
           
Sbjct: 464  408

Query: 246  305
           
Sbjct: 524  408

Query: 306  365
           
Sbjct: 584  408

Query: 366  375
           
Sbjct: 644  408

[Close]

BLAST of CU098916 vs. NCBI nr
Match: gi|645260918|ref|XP_008236046.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Prunus mume])

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-36
Identity = 80/130 (61.54%), Postives = 95/130 (73.08%), Query Frame = 3


Query: 6   NFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDEE----- 65
           N S+ N  +AGDALH MTPD+GQGGC+ALED V+LARCLGEA+ +N  GE +D+E     
Sbjct: 283 NISKGNACVAGDALHPMTPDIGQGGCAALEDSVVLARCLGEALLKNSGGERKDKEGEEGK 342

Query: 66  -EYKRIEKGLEKYAKERRWRSIKLIIASDVVGSIQESKGKVMNYLRDNILADSLVGVLMK 125
            EY+RIE GL KYA ERRWRS  LI  S VVG +QES GK MN+ RD  L+  L G+L+K
Sbjct: 343 EEYERIEMGLNKYANERRWRSFDLISTSRVVGFLQESNGKFMNFFRDKFLSPILAGLLLK 402

Query: 126 ISDFDCGTLS 185
            SDFDCG LS
Sbjct: 403 KSDFDCGKLS 412

Query: 186  245
           
Sbjct: 463  412

Query: 246  305
           
Sbjct: 523  412

Query: 306  365
           
Sbjct: 583  412

Query: 366  378
           
Sbjct: 643  412

The following BLAST results are available for this feature:

BLAST of CU098916 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HPXO_ACIAD	1.4e-06	34.18	FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD4...	[more]

BLAST of CU098916 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2K7_CUCSA	7.3e-63	99.20	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G014500 PE=4 SV=1	[more]
A0A072W2C4_MEDTR	1.5e-39	67.48	FAD/NAD(P)-binding oxidoreductase family protein OS=Medicago truncatula GN=MTR_1...	[more]
A0A072W2C4_MEDTR	3.5e-33	60.33	FAD/NAD(P)-binding oxidoreductase family protein OS=Medicago truncatula GN=MTR_1...	[more]
U5GJH2_POPTR	1.2e-36	59.52	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s18310g PE=4 SV=1	[more]
A0A067FL81_CITSI	2.0e-36	60.00	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g015061mg PE=4 SV=1	[more]

BLAST of CU098916 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449454223\|ref\|XP_004144855.1\|	4.0e-62	99.20	PREDICTED: zeaxanthin epoxidase, chloroplastic [Cucumis sativus]	[more]
gi\|659094208\|ref\|XP_008447940.1\|	7.0e-59	95.16	PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Cucumis melo]	[more]
gi\|922408735\|ref\|XP_013470337.1\|	8.0e-39	67.48	FAD/NAD(P)-binding oxidoreductase family protein [Medicago truncatula]	[more]
gi\|802675046\|ref\|XP_012081816.1\|	2.9e-36	61.60	PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Jatropha curcas]	[more]
gi\|645260918\|ref\|XP_008236046.1\|	3.7e-36	61.54	PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002938	FAD-bd
IPR023753	FAD/NAD-binding_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0071949	FAD binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa7G014500	Csa7G014500	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
FKNP3UI02K1IKU	FKNP3UI02K1IKU	EST
FKNP3UI02L6CZY	FKNP3UI02L6CZY	EST
FKNP3UI02OTMZ4	FKNP3UI02OTMZ4	EST
FKNP3UI02OZLYX	FKNP3UI02OZLYX	EST
G0137371	G0137371	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 3..46 score: 1.
IPR023753	FAD/NAD(P)-binding domain	GENE3D	G3DSA:3.50.50.60		coord: 3..85 score: 2.
IPR023753	FAD/NAD(P)-binding domain	unknown	SSF51905	FAD/NAD(P)-binding domain	coord: 3..106 score: 1.9
None	No IPR available	PANTHER	PTHR13789	MONOOXYGENASE	coord: 3..126 score: 1.1
None	No IPR available	PANTHER	PTHR13789:SF185	FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN	coord: 3..126 score: 1.1