CU089705 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU089705
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Lipoxygenase
Location	Chr4 : 11216981 .. 11217733 (+)
Sequence length	592

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TTATGACGACAGCAGGAGAATCTTCTTCACCACTAAGTCATATTTGCCATCCAACACTCCTGAGGGCCTAAAGAGACTTAGAGAAGAAGAGCTCAAAGTTTTGCAAGGGGATGGCGTTGGCAAGCGTGAGAACCACGAAAGGATATATGATTATGATGTCTACAATGATCTAGGCGATCCCGATAGAGATCTCGCTCTCAAAAGACCTGTGCTTGGTGGAAAACAATTCCCTTATCCTCGTCGATGTAGAACCGGACGCCCACGTTCTAAAAGAGATGCTTTATCTGAATCAAGAAGTTCAGACAATTATGTGCCTCGAGATGAAGCATTCTCACCAGTGAAACAAGCAACTTTTAGTGTGAGGACACTGACCTCAGTGCTAAAGGGGCTAATACCGGCTTTTGGAGTCAGTTTCTACTGACACTGATATACGTTTCCCACATTTCCCTGCAATAGATGATCTTTATGATAATGGAGTTCCATTGCCTGCTGCCAAAGACGGTTTAAGGCAGTTGGCTACTGTCCTTCCAAGGCTTATTGACACTGTTGCTGATAGGGCTGAGGATATCTTGCGTTTCGTTCCTCCTAGAAA

Library


Library category	ESTs
gynoecious flower library	1
Cucumber female flower buds	1

Homology

[Close]

BLAST of CU089705 vs. Swiss-Prot
Match: LOX21_SOLTU (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.0e-38
Identity = 84/146 (57.53%), Postives = 97/146 (66.44%), Query Frame = 2


Query: 14  RRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPDRDL 73
           +RIFFT KSYLPS TP G           L+GDG+G+R+  ERIYDYDVYNDLG+ D + 
Sbjct: 195 KRIFFTNKSYLPSQTPSGVSRLREEELVTLRGDGIGERKVFERIYDYDVYNDLGEADSNN 254

Query: 74  A-LKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVRTL 133
              KRPVLGGK+ PYPRRC+TGRPRSK+D LSE+RS+  YVPRDEAFS VK   FS  T+
Sbjct: 255 DDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETRSTFVYVPRDEAFSEVKSVAFSGNTV 314

Query: 134 TSVLKGLIPAFGVSFY*H*YTFPTFP 193
            SVL  ++PA           FP FP
Sbjct: 315 YSVLHAVVPALESVVTDPNLGFPHFP 340

Query: 194  253
           
Sbjct: 375  340

Query: 254  313
           
Sbjct: 435  340

Query: 314  373
           
Sbjct: 495  340

Query: 374  433
           
Sbjct: 555  340

Query: 434  449
           
Sbjct: 615  340

HSP 2 Score: 63.2 bits (152), Expect = 3.8e-09
Identity = 31/63 (49.21%), Postives = 39/63 (61.90%), Query Frame = 3


Query: 402 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV 461
           LESV TD ++ FPHFPAID L++ GV LP   D    L  V+PRLI  ++D  +D+L F 
Sbjct: 325 LESVVTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLIKAISDTRKDVLLFE 384

Query: 462 PPR 521
            P+
Sbjct: 385 SPQ 387

Query: 522  581
           
Sbjct: 445  387

Query: 582  591
           
Sbjct: 505  387

[Close]

BLAST of CU089705 vs. Swiss-Prot
Match: LOX23_HORVU (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 9.5e-37
Identity = 76/132 (57.58%), Postives = 88/132 (66.67%), Query Frame = 2


Query: 5   DDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPD 64
           D   R FFT KSYLPS TP G           L+GDG  +R+ HER+YDYD YNDLGDPD
Sbjct: 191 DPEPRAFFTVKSYLPSQTPPGIEALRKKELETLRGDGHSERKFHERVYDYDTYNDLGDPD 250

Query: 65  RDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVR 124
           +++  KRPVLG K+ PYPRRCRTGRP++  D  +E+RSS  YVPRDE FS VK  TFS  
Sbjct: 251 KNIDHKRPVLGTKEHPYPRRCRTGRPKTLYDPETETRSSPVYVPRDEQFSDVKGRTFSAT 310

Query: 125 TLTSVLKGLIPA 184
           TL S L  ++PA
Sbjct: 311 TLRSGLHAILPA 322

Query: 185  244
           
Sbjct: 371  322

Query: 245  304
           
Sbjct: 431  322

Query: 305  364
           
Sbjct: 491  322

Query: 365  401
           
Sbjct: 551  322

HSP 2 Score: 48.5 bits (114), Expect = 9.6e-05
Identity = 23/54 (42.59%), Postives = 29/54 (53.70%), Query Frame = 3


Query: 435 FPHFPAIDDLYDNGVPLPAAKDG---LRQLATVLPRLIDTVADRAEDILRFVPP 494
           F HFPAID LY +G+PLP    G      +  V+PR++  + D  E +LRF  P
Sbjct: 333 FSHFPAIDALYSDGIPLPVDGHGGNSFNVINDVIPRVVQMIEDTTEHVLRFEVP 386

Query: 495  554
           
Sbjct: 393  386

Query: 555  588
           
Sbjct: 453  386

[Close]

BLAST of CU089705 vs. Swiss-Prot
Match: LOXC1_ORYSJ (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 1.4e-35
Identity = 75/131 (57.25%), Postives = 89/131 (67.94%), Query Frame = 2


Query: 11  SRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPDRD 70
           S+RIFF  K+YLP  TP G            +GDG G+RE  +R+YDYDVYNDLG+PD +
Sbjct: 216 SKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRGDGTGEREADDRVYDYDVYNDLGNPDSN 275

Query: 71  LALKRPVLGG-KQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVRT 130
             L RPVLGG KQFPYPRRCRTGRP SK+D  SE+R  + YVPRDE FSP K+  F  +T
Sbjct: 276 GDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETRKGNVYVPRDEEFSPEKEDYFLRKT 335

Query: 131 LTSVLKGLIPA 190
           + SVL+  +PA
Sbjct: 336 VGSVLQAAVPA 346

Query: 191  250
           
Sbjct: 396  346

Query: 251  310
           
Sbjct: 456  346

Query: 311  370
           
Sbjct: 516  346

Query: 371  401
           
Sbjct: 576  346

HSP 2 Score: 42.4 bits (98), Expect = 6.9e-03
Identity = 25/55 (45.45%), Postives = 33/55 (60.00%), Query Frame = 3


Query: 426 DIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADR-AEDILRFVPP 485
           ++ FP F  ID L+++GV LP   D L  L +V+PRL++ + D  AE ILRF  P
Sbjct: 358 NLPFPSFFVIDKLFEDGVELPGV-DKLNFLESVVPRLLEHLRDTPAEKILRFETP 411

Query: 486  545
           
Sbjct: 418  411

Query: 546  588
           
Sbjct: 478  411

[Close]

BLAST of CU089705 vs. Swiss-Prot
Match: LOXC2_ORYSJ (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.4e-34
Identity = 75/130 (57.69%), Postives = 88/130 (67.69%), Query Frame = 2


Query: 14  RRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPDRDL 73
           +RIFF  K+YLP  TP G            +G+G G+RE  +R+YDYDVYNDLG+PD + 
Sbjct: 235 KRIFFA-KAYLPGQTPAGLRSYREEDLKQKRGNGAGQREADDRVYDYDVYNDLGNPDSNG 294

Query: 74  ALKRPVLGG-KQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVRTL 133
            L RPVLGG KQFPYPRRCRTGRP SK+D  SE+R  + YVPRDE FS VK A F ++TL
Sbjct: 295 DLARPVLGGSKQFPYPRRCRTGRPPSKKDPKSETRKGNVYVPRDEEFSEVKNAQFLLKTL 354

Query: 134 TSVLKGLIPA 193
            SVL   +PA
Sbjct: 355 QSVLHAAVPA 363

Query: 194  253
           
Sbjct: 415  363

Query: 254  313
           
Sbjct: 475  363

Query: 314  373
           
Sbjct: 535  363

Query: 374  401
           
Sbjct: 595  363

HSP 2 Score: 34.7 bits (78), Expect = 1.4e+00
Identity = 21/55 (38.18%), Postives = 31/55 (56.36%), Query Frame = 3


Query: 426 DIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAED-ILRFVPP 485
           ++ FP F  ID L+++GV LP   + L  L +++PRL++ + D   D IL F  P
Sbjct: 375 NLPFPSFFVIDKLFEDGVELPGV-EKLGFLHSIVPRLLELLRDSPGDKILLFDTP 428

Query: 486  545
           
Sbjct: 435  428

Query: 546  588
           
Sbjct: 495  428

[Close]

BLAST of CU089705 vs. Swiss-Prot
Match: LOX21_HORVU (Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 71/129 (55.04%), Postives = 83/129 (64.34%), Query Frame = 2


Query: 14  RRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPDRD- 73
           +R+FF   SYLPS TP+G          +L+G G G+R+ H+RIYDYDVYNDLG+PD D 
Sbjct: 212 KRVFFPAHSYLPSQTPKGVEGLRKRELEILRGTGCGERKEHDRIYDYDVYNDLGNPDDDN 271

Query: 74  LALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDN--YVPRDEAFSPVKQATFSVR 133
               RPVLGGK+ PYPRRCRTGRPRSK+D  SE RS     YVPRDEAF+  K   F  +
Sbjct: 272 NPTTRPVLGGKEHPYPRRCRTGRPRSKKDPFSEERSHKEHIYVPRDEAFTERKMGAFDTK 331

Query: 134 TLTSVLKGL 193
              S L  L
Sbjct: 332 KFMSQLHAL 340

Query: 194  253
           
Sbjct: 392  340

Query: 254  313
           
Sbjct: 452  340

Query: 314  373
           
Sbjct: 512  340

Query: 374  392
           
Sbjct: 572  340

[Close]

BLAST of CU089705 vs. TrEMBL
Match: A0A0A0L1W4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 8.3e-64
Identity = 127/149 (85.23%), Postives = 127/149 (85.23%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           YDDSRRIFFTTKSYLPSNTPEG          VLQGDGVGKRENHERIYDYDVYNDLGDP
Sbjct: 196 YDDSRRIFFTTKSYLPSNTPEGLKRLREEELKVLQGDGVGKRENHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           RTLTSVLKGLIPA           FP FP
Sbjct: 316 RTLTSVLKGLIPALESVSTDTDIRFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. TrEMBL
Match: A0A0A0L1W4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-26
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 3


Query: 402 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV 461
           LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV
Sbjct: 329 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV 388

Query: 462 PP 521
           PP
Sbjct: 389 PP 390

Query: 522  581
           
Sbjct: 449  390

Query: 582  588
           
Sbjct: 509  390

HSP 2 Score: 226.1 bits (575), Expect = 3.7e-56
Identity = 113/149 (75.84%), Postives = 120/149 (80.54%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           + DS+RIFFTTKSYLPSNTPEG           LQGDGVG+RE HERIYDYDVYNDLGDP
Sbjct: 196 HPDSKRIFFTTKSYLPSNTPEGLKRLREEELKALQGDGVGQREVHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           D++L LKRPVLGGKQFPYPRRCRTGRPRSK D LSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DKNLKLKRPVLGGKQFPYPRRCRTGRPRSKEDPLSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           +TL S+LKGLIPA           FP FP
Sbjct: 316 KTLNSLLKGLIPALESVSTDTDIRFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. TrEMBL
Match: A0A125S6K7_CUCME (Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX10 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-22
Identity = 54/62 (87.10%), Postives = 57/62 (91.94%), Query Frame = 3


Query: 402 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV 461
           LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAK G +Q+AT LPRLID+V DRAED LRFV
Sbjct: 329 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKGGWKQIATALPRLIDSVVDRAEDFLRFV 388

Query: 462 PP 521
           PP
Sbjct: 389 PP 390

Query: 522  581
           
Sbjct: 449  390

Query: 582  588
           
Sbjct: 509  390

HSP 2 Score: 178.7 bits (452), Expect = 6.9e-42
Identity = 88/147 (59.86%), Postives = 101/147 (68.71%), Query Frame = 2


Query: 5   DDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPD 64
           D  +R+FFT KSYLPS TP+G          +L+GDG G+R+  ER+YDYDVYNDLGDPD
Sbjct: 196 DKRKRLFFTNKSYLPSQTPDGLRRLREEELVLLRGDGQGERKKGERVYDYDVYNDLGDPD 255

Query: 65  RDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVR 124
            D   KRPVLGGKQ PYPRRCRTGRPR K D LSESRSS  YVPRDE FS VK+ TFS +
Sbjct: 256 NDSDTKRPVLGGKQLPYPRRCRTGRPRCKTDPLSESRSSSVYVPRDEEFSEVKELTFSAK 315

Query: 125 TLTSVLKGLIPAFGVSFY*H*YTFPTF 184
           TL S L  ++P+   S       FP F
Sbjct: 316 TLYSALHAVLPSIETSIVDSTLPFPFF 342

Query: 185  244
           
Sbjct: 376  342

Query: 245  304
           
Sbjct: 436  342

Query: 305  364
           
Sbjct: 496  342

Query: 365  424
           
Sbjct: 556  342

Query: 425  446
           
Sbjct: 616  342

[Close]

BLAST of CU089705 vs. TrEMBL
Match: A0A067JBW7_JATCU (Lipoxygenase OS=Jatropha curcas GN=JCGZ_07923 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 6.2e-03
Identity = 25/63 (39.68%), Postives = 37/63 (58.73%), Query Frame = 3


Query: 402 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDILRFV 461
           +E+   D+ + FP F  IDDL++ GV LP  K   + L  +LP +I  + D A+ ILRF 
Sbjct: 328 IETSIVDSTLPFPFFSEIDDLFNEGVNLPPLKG--QGLKHILPNIIKAIGDEADSILRFE 387

Query: 462 PPR 521
            P+
Sbjct: 388 TPQ 388

Query: 522  581
           
Sbjct: 448  388

Query: 582  591
           
Sbjct: 508  388

HSP 2 Score: 176.8 bits (447), Expect = 2.6e-41
Identity = 85/147 (57.82%), Postives = 104/147 (70.75%), Query Frame = 2


Query: 5   DDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPD 64
           D  +R+FFT KSYLPS TP G            +G+G G+R+ ++RIYDYD+YNDLGDPD
Sbjct: 128 DSEKRVFFTNKSYLPSQTPNGLKRLREKDLAENRGNGQGERKTYDRIYDYDMYNDLGDPD 187

Query: 65  RDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVR 124
            D+ LKRPVLGGK+ PYPRRCRTGR R+K+D LSESRSSD YVPRDEAFS VK  TFS +
Sbjct: 188 SDIGLKRPVLGGKEHPYPRRCRTGRARTKKDPLSESRSSDIYVPRDEAFSNVKNQTFSAK 247

Query: 125 TLTSVLKGLIPAFGVSFY*H*YTFPTF 184
           T+ SVL  ++P+   +       FP F
Sbjct: 248 TVYSVLHAVVPSLETAIVDENLGFPYF 274

Query: 185  244
           
Sbjct: 308  274

Query: 245  304
           
Sbjct: 368  274

Query: 305  364
           
Sbjct: 428  274

Query: 365  424
           
Sbjct: 488  274

Query: 425  446
           
Sbjct: 548  274

[Close]

BLAST of CU089705 vs. TrEMBL
Match: Q0ZDG4_ACTDE (Lipoxygenase 6 (Fragment) OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 6.9e-02
Identity = 22/57 (38.60%), Postives = 33/57 (57.89%), Query Frame = 3


Query: 402 LESVSTDTDIRFPHFPAIDDLYDNGVPLPAAKDGLRQLATVLPRLIDTVADRAEDIL 461
           LE+   D ++ FP+F  ID L++ GV +P  K+    L  +LPR+I  V D   D+L
Sbjct: 260 LETAIVDENLGFPYFTRIDSLFNEGVNIPELKNNSGFLRNLLPRIIKFVGDAQADLL 316

Query: 462  521
           
Sbjct: 320  316

Query: 522  573
           
Sbjct: 380  316

HSP 2 Score: 176.8 bits (447), Expect = 2.6e-41
Identity = 88/144 (61.11%), Postives = 100/144 (69.44%), Query Frame = 2


Query: 14  RRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPDRDL 73
           +RIFFT KSYLP  TP G           LQGDG G+R+ H+RIYDYDVYND+GDPD + 
Sbjct: 199 KRIFFTNKSYLPGQTPSGLKRLRKKELENLQGDGQGERKTHDRIYDYDVYNDIGDPDSNS 258

Query: 74  ALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVRTLT 133
            LKRPVLGGK+ PYPRRCRTGRPR K D LSESRSS  YVPRDE FS VKQ TFS +T+ 
Sbjct: 259 ELKRPVLGGKKHPYPRRCRTGRPRCKTDPLSESRSSTVYVPRDEKFSEVKQLTFSAKTVY 318

Query: 134 SVLKGLIPAFGVSFY*H*YTFPTF 193
           SVL  L+P+   +       FP F
Sbjct: 319 SVLHALVPSLQTAIVDTDLGFPYF 342

Query: 194  253
           
Sbjct: 379  342

Query: 254  313
           
Sbjct: 439  342

Query: 314  373
           
Sbjct: 499  342

Query: 374  433
           
Sbjct: 559  342

Query: 434  446
           
Sbjct: 619  342

[Close]

BLAST of CU089705 vs. NCBI nr
Match: gi|778693116|ref|XP_004142135.2| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-63
Identity = 127/149 (85.23%), Postives = 127/149 (85.23%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           YDDSRRIFFTTKSYLPSNTPEG          VLQGDGVGKRENHERIYDYDVYNDLGDP
Sbjct: 196 YDDSRRIFFTTKSYLPSNTPEGLKRLREEELKVLQGDGVGKRENHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           RTLTSVLKGLIPA           FP FP
Sbjct: 316 RTLTSVLKGLIPALESVSTDTDIRFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. NCBI nr
Match: gi|700198983|gb|KGN54141.1| (hypothetical protein Csa_4G288610 [Cucumis sativus])

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-63
Identity = 127/149 (85.23%), Postives = 127/149 (85.23%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           YDDSRRIFFTTKSYLPSNTPEG          VLQGDGVGKRENHERIYDYDVYNDLGDP
Sbjct: 196 YDDSRRIFFTTKSYLPSNTPEGLKRLREEELKVLQGDGVGKRENHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           RTLTSVLKGLIPA           FP FP
Sbjct: 316 RTLTSVLKGLIPALESVSTDTDIRFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. NCBI nr
Match: gi|987644663|gb|AME15768.1| (lipoxygenase 10 [Cucumis melo var. makuwa])

HSP 1 Score: 226.1 bits (575), Expect = 5.4e-56
Identity = 113/149 (75.84%), Postives = 120/149 (80.54%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           + DS+RIFFTTKSYLPSNTPEG           LQGDGVG+RE HERIYDYDVYNDLGDP
Sbjct: 196 HPDSKRIFFTTKSYLPSNTPEGLKRLREEELKALQGDGVGQREVHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           D++L LKRPVLGGKQFPYPRRCRTGRPRSK D LSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DKNLKLKRPVLGGKQFPYPRRCRTGRPRSKEDPLSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           +TL S+LKGLIPA           FP FP
Sbjct: 316 KTLNSLLKGLIPALESVSTDTDIRFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. NCBI nr
Match: gi|659097665|ref|XP_008449747.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 226.1 bits (575), Expect = 5.4e-56
Identity = 113/149 (75.84%), Postives = 120/149 (80.54%), Query Frame = 2


Query: 2   YDDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDP 61
           + DS+RIFFTTKSYLPSNTPEG           LQGDGVG+RE HERIYDYDVYNDLGDP
Sbjct: 196 HPDSKRIFFTTKSYLPSNTPEGLKRLREEELKALQGDGVGQREVHERIYDYDVYNDLGDP 255

Query: 62  DRDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSV 121
           D++L LKRPVLGGKQFPYPRRCRTGRPRSK D LSESRSSDNYVPRDEAFSPVKQATFSV
Sbjct: 256 DKNLKLKRPVLGGKQFPYPRRCRTGRPRSKEDPLSESRSSDNYVPRDEAFSPVKQATFSV 315

Query: 122 RTLTSVLKGLIPAFGVSFY*H*YTFPTFP 181
           +TL S+LKGLIPA           FP FP
Sbjct: 316 KTLNSLLKGLIPALESVSTDTDICFPHFP 344

Query: 182  241
           
Sbjct: 376  344

Query: 242  301
           
Sbjct: 436  344

Query: 302  361
           
Sbjct: 496  344

Query: 362  421
           
Sbjct: 556  344

Query: 422  449
           
Sbjct: 616  344

[Close]

BLAST of CU089705 vs. NCBI nr
Match: gi|802796518|ref|XP_012092789.1| (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Jatropha curcas])

HSP 1 Score: 178.7 bits (452), Expect = 9.8e-42
Identity = 88/147 (59.86%), Postives = 101/147 (68.71%), Query Frame = 2


Query: 5   DDSRRIFFTTKSYLPSNTPEGXXXXXXXXXXVLQGDGVGKRENHERIYDYDVYNDLGDPD 64
           D  +R+FFT KSYLPS TP+G          +L+GDG G+R+  ER+YDYDVYNDLGDPD
Sbjct: 196 DKRKRLFFTNKSYLPSQTPDGLRRLREEELVLLRGDGQGERKKGERVYDYDVYNDLGDPD 255

Query: 65  RDLALKRPVLGGKQFPYPRRCRTGRPRSKRDALSESRSSDNYVPRDEAFSPVKQATFSVR 124
            D   KRPVLGGKQ PYPRRCRTGRPR K D LSESRSS  YVPRDE FS VK+ TFS +
Sbjct: 256 NDSDTKRPVLGGKQLPYPRRCRTGRPRCKTDPLSESRSSSVYVPRDEEFSEVKELTFSAK 315

Query: 125 TLTSVLKGLIPAFGVSFY*H*YTFPTF 184
           TL S L  ++P+   S       FP F
Sbjct: 316 TLYSALHAVLPSIETSIVDSTLPFPFF 342

Query: 185  244
           
Sbjct: 376  342

Query: 245  304
           
Sbjct: 436  342

Query: 305  364
           
Sbjct: 496  342

Query: 365  424
           
Sbjct: 556  342

Query: 425  446
           
Sbjct: 616  342

The following BLAST results are available for this feature:

BLAST of CU089705 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LOX21_SOLTU	1.0e-38	57.53	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum GN=LOX2.1 PE=...	[more]
LOX23_HORVU	9.5e-37	57.58	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare GN=LOX2.3 PE=1 SV=1	[more]
LOXC1_ORYSJ	1.4e-35	57.25	Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX1 PE=2 SV...	[more]
LOXC2_ORYSJ	3.4e-34	57.69	Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica GN=CM-LOX...	[more]
LOX21_HORVU	1.1e-32	55.04	Lipoxygenase 2.1, chloroplastic OS=Hordeum vulgare GN=LOX2.1 PE=1 SV=1	[more]

BLAST of CU089705 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1W4_CUCSA	8.3e-64	85.23	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1	[more]
A0A0A0L1W4_CUCSA	1.8e-26	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G288610 PE=4 SV=1	[more]
A0A125S6K7_CUCME	1.6e-22	87.10	Lipoxygenase OS=Cucumis melo var. makuwa GN=LOX10 PE=2 SV=1	[more]
A0A067JBW7_JATCU	6.2e-03	39.68	Lipoxygenase OS=Jatropha curcas GN=JCGZ_07923 PE=3 SV=1	[more]
Q0ZDG4_ACTDE	6.9e-02	38.60	Lipoxygenase 6 (Fragment) OS=Actinidia deliciosa PE=2 SV=1	[more]

BLAST of CU089705 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778693116\|ref\|XP_004142135.2\|	1.2e-63	85.23	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis sativus]	[more]
gi\|700198983\|gb\|KGN54141.1\|	1.2e-63	85.23	hypothetical protein Csa_4G288610 [Cucumis sativus]	[more]
gi\|987644663\|gb\|AME15768.1\|	5.4e-56	75.84	lipoxygenase 10 [Cucumis melo var. makuwa]	[more]
gi\|659097665\|ref\|XP_008449747.1\|	5.4e-56	75.84	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo]	[more]
gi\|802796518\|ref\|XP_012092789.1\|	9.8e-42	59.86	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000907	LipOase
IPR001024	PLAT/LH2_dom
IPR001246	LipOase_plant
IPR013819	LipOase_C
IPR027433	Lipoxygenase_dom_3

Vocabulary: **Molecular Function**
Term	Definition
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872	metal ion binding
GO:0005515	protein binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function	GO:0005515	protein binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa4G288610	Csa4G288610	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
G0084307	G0084307	EST
csa01-3ms2-g05	csa01-3ms2-g05	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 3..153 score: 2.2E-71coord: 188..197 score: 2.2
IPR001024	PLAT/LH2 domain	GENE3D	G3DSA:2.60.60.20		coord: 4..33 score: 8.2
IPR001246	Lipoxygenase, plant	PRINTS	PR00468	PLTLPOXGNASE	coord: 78..97 score: 5.8E-33coord: 7..28 score: 5.8E-33coord: 46..62 score: 5.8E-33coord: 191..198 score: 5.8E-33coord: 143..164 score: 5.8
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 23..197 score: 6.8
IPR013819	Lipoxygenase, C-terminal	PROFILE	PS51393	LIPOXYGENASE_3	coord: 14..198 score: 51
IPR013819	Lipoxygenase, C-terminal	unknown	SSF48484	Lipoxigenase	coord: 16..196 score: 1.31
IPR027433	Lipoxygenase, domain 3	GENE3D	G3DSA:4.10.372.10		coord: 124..197 score: 2.
None	No IPR available	GENE3D	G3DSA:4.10.375.10		coord: 35..123 score: 5.0
None	No IPR available	PANTHER	PTHR11771:SF42	PROTEIN C13C4.7	coord: 188..197 score: 2.2E-71coord: 3..153 score: 2.2