MELO3C018756T1 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C018756T1
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionLipoyl synthase
Locationchr1 : 2610048 .. 2612683 (-)
Sequence length1590
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACGAACGCCCTTCAACTATCCAAATCCACCCAAATTCCGGTTAGGCTTCTTCTCCGAGATCGACGACCACGATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTAGTAGTAATTCAGTTTATTCAATCCATTTCTTTATGCTTATTTAATTGCCAATTAACGACTGAAGAACCATAATTTCTTGTTATTCTCCTGTGTGTAATGTCAATGTATATATAATTTAGTGGGGAACGTGAATGGATTTAGAACTGATTGTTTTTGTCATAGAAAATGACCCTGTTGATCACTTTGATATCATCGCGGTGCCTCAGAATTTCTAATTCCATTGCTGTGTGGGATTTTTTGAACACCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGGTACTAATTCCCTAGTTTCTATTACGCATAACAGTGTCTAGTTCATTGTTGCATTCAAGGATCCTTGATTTAAGGAACCTTATTTTATTTGGTTTCGGTGCAAATTTAGTGAAAGCTACTAATTTTACCCTGCACTGTTGATGTCGCCAAATCAGCATATGATATGGATGTGCTTCTGACTATGTTTTTCAGTGTGATTTAATCTCTTCTTTCATGTTGCTTATGCTTAACTAATTGATTTTGCAGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGTACTCTCTCTTTCTAATTATTTGAACAATTTGATTGTGTACTGTCTCCTCTGTTCTGCTAGTGAAAAATGATGATCGCTCAATCAATGCCTTAAAGGCTAAAGTCAACTTGATCTTTTTTTAAAAAAACTATAATATTGGGAATATTGTATCATTTTCCCATAAGACTAGGATGAATCAAAGTGCATATCTTTTTCTCTAACCTAAATATCTATTTTAAGCACACTTTAAAAGTTAATCCAAACAGGTCTTCTTGGATTTGTACGGTCTGAATAACTTTCATTTGCATTTTCCTTCTCTTGGATCAGTATCGTTGTAATTTTATGTGTGTTTGATTTGTTAGAGGGAGTCCATGTTTTACTTTAAAACATATACTAATTCCCACTTTGATTCATAACCCTCACTTTTAAGTTTATAGGAAATCTCCATGATCAAGTATTACTTATTATATGATCAATTGTTTTTTGATGGCTGGAGTTGCCTGCCATGCCTTGAGGGTGCAACAATTATCTAAAAAATCCATTTTTTTTTTTGTTTGGTTGTGGCAGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGATTATTGTTACCATTTCTATTGGTCATCTGTCCCCAACAATCCCTACAAAATTTAACCATGTTGCATGTAAGTTGACATATAAGCAAATTGGACTTTTGATTTGATGGATTTGTCCAGTCGTTTTGTCAAATTCTTGTGCTCCTGATCCGCTGCAGATGTATTGTCACTGTAGCCATAAATTTTTGCTCGACTCCTATATAATATCTCCCCTTCATCCTGTTCTGGCTGATCAGCATAGGGGTATTACTATTCTTGTAATATTGGAATTGGAATTTTATTTATTTGCTATTTGTATAGGTTTACGTTTGTACCATTTTGCTTGTTCATACAAAAACTCAATTATTGTATTTCAATGCAAAAGCAGGTTCAACT

mRNA sequence

AACGAACGCCCTTCAACTATCCAAATCCACCCAAATTCCGGTTAGGCTTCTTCTCCGAGATCGACGACCACGATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGATTATTGTTACCATTTCTATTGGTCATCTGTCCCCAACAATCCCTACAAAATTTAACCATGTTGCATGTAAGTTGACATATAAGCAAATTGGACTTTTGATTTGATGGATTTGTCCAGTCGTTTTGTCAAATTCTTGTGCTCCTGATCCGCTGCAGATGTATTGTCACTGTAGCCATAAATTTTTGCTCGACTCCTATATAATATCTCCCCTTCATCCTGTTCTGGCTGATCAGCATAGGGGTATTACTATTCTTGTAATATTGGAATTGGAATTTTATTTATTTGCTATTTGTATAGGTTTACGTTTGTACCATTTTGCTTGTTCATACAAAAACTCAATTATTGTATTTCAATGCAAAAGCAGGTTCAACT

Coding sequence (CDS)

ATGAACAAACGTTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCATTCTCTTCTTCATCTTCTTCCACCTCCGCCTCTGCCACATCTTCCCAATTCCCGCGCACGTTGGCCGGTCTCCGGGAACGACTCGCAGCGGAGTCCCCATCACTTTCCGACTTCGTTGATCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGAGGCGAGAAATATGTTCAAATCAAGAAGAAGCTCCGGGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGTTGCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCGGATCCAAATGAGCCGACAAATGTTGCTGAAGCAATTGCATCATGGGGTTTGGATTATGTGGTCATTACTAGTGTCGATCGCGACGATTTACCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCAAATATGCTGATCGAGGCATTGGTTCCTGATTTTCGAGGAGACTCTGGCTGTGTGGAGAAAGTTGCAAGGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAAGGAGCAGTTAGGGATCACCGTGCTAATTTCAAGCAATCATTAGACGTTCTAGTTATGGCCAAAGAGTTTTCTCCTTCTGGAACTCTTACAAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACATTTGGGCAGTATATGAGACCTTCAAAACGCCATATGCCTGTATCAGAATACATTACTCCTGAGGCTTTTGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGATATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACTTTGA

Protein sequence

MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQL*
BLAST of MELO3C018756T1 vs. Swiss-Prot
Match: LIAS2_PEA (Lipoyl synthase 2, mitochondrial OS=Pisum sativum GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.1e-185
Identity = 316/375 (84.27%), Postives = 347/375 (92.53%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of MELO3C018756T1 vs. Swiss-Prot
Match: LIAS1_PEA (Lipoyl synthase 1, mitochondrial OS=Pisum sativum GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 1.4e-185
Identity = 316/375 (84.27%), Postives = 346/375 (92.27%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

BLAST of MELO3C018756T1 vs. Swiss-Prot
Match: LIAS_POPTR (Lipoyl synthase, mitochondrial OS=Populus trichocarpa GN=LIP1 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 7.0e-185
Identity = 316/366 (86.34%), Postives = 340/366 (92.90%), Query Frame = 1

Query: 13  KSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTK 72
           K+ +R+FSSS+  +S      QF +TLAGLR RLA ESP+LSDF+ LQS+++YSVEVGTK
Sbjct: 19  KAKNRTFSSSTVESSTKQPP-QFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTK 78

Query: 73  KKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 132
           KKPLPKPKWM+E++PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI
Sbjct: 79  KKPLPKPKWMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 138

Query: 133 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGS 192
           MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDL DQGS
Sbjct: 139 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGS 198

Query: 193 GHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRD 252
           GHFAETV KLK LKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNIETVEELQ +VRD
Sbjct: 199 GHFAETVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRD 258

Query: 253 HRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQY 312
           HRANFKQSLDVL+MAKE++P GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQY
Sbjct: 259 HRANFKQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQY 318

Query: 313 MRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRA 372
           MRPSKRHMPVSEYITP+AFEKY+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDR+
Sbjct: 319 MRPSKRHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRS 378

Query: 373 ASNSQP 379
            S+  P
Sbjct: 379 VSSQLP 383

BLAST of MELO3C018756T1 vs. Swiss-Prot
Match: LIAS_MEDTR (Lipoyl synthase, mitochondrial OS=Medicago truncatula GN=LIP1 PE=2 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-183
Identity = 310/375 (82.67%), Postives = 347/375 (92.53%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++A+ LKS ++ FS ++++T+ + +SS+FP+ L  LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+++YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANF QSLDVL MAK+++P+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASN 376
           FYIKSMI+SDRA S+
Sbjct: 362 FYIKSMIDSDRAVSS 376

BLAST of MELO3C018756T1 vs. Swiss-Prot
Match: LIAS_ARATH (Lipoyl synthase, mitochondrial OS=Arabidopsis thaliana GN=LIP1 PE=2 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 1.8e-180
Identity = 311/376 (82.71%), Postives = 338/376 (89.89%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESPSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFIH-- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  -GDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV++SGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL MAKE++P+GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNS 377
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

BLAST of MELO3C018756T1 vs. TrEMBL
Match: A0A0A0K1R3_CUCSA (Lipoyl synthase, mitochondrial OS=Cucumis sativus GN=LIP1 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 1.7e-214
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of MELO3C018756T1 vs. TrEMBL
Match: A0A151QZP0_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_043119 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 1.4e-187
Identity = 326/377 (86.47%), Postives = 353/377 (93.63%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R F SSS++T A+   SQFP+TLAGLR RLA ESPSL+DF+ L+
Sbjct: 2   MHSRFRSVAGNIKCAARLFCSSSTTTPAAP--SQFPQTLAGLRARLAEESPSLTDFIALK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SESAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKPNMLIEALVPDFRGD+ CVEKVA+SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPNMLIEALVPDFRGDADCVEKVAKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 242 ETVEELQNVVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASNSQ 378
           FYIKSMIESDRAAS S+
Sbjct: 362 FYIKSMIESDRAASPSK 376

BLAST of MELO3C018756T1 vs. TrEMBL
Match: A0A0R4J536_SOYBN (Lipoyl synthase, mitochondrial OS=Glycine max GN=LIP1 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 4.0e-187
Identity = 327/373 (87.67%), Postives = 350/373 (93.83%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R F SSS+ T A+A S Q P+TLAGLR RLA ESPSLSDFV L+
Sbjct: 3   MHSRIRSVAGNIKCAARLFCSSSTITPAAAPS-QLPQTLAGLRARLAEESPSLSDFVALK 62

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 63  SDSAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 122

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 123 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 182

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF+ETVQKLK LKPNMLIEALVPDFRGD+ CVEKVA+SGLDVFAHNI
Sbjct: 183 SVDRDDLPDQGSGHFSETVQKLKALKPNMLIEALVPDFRGDADCVEKVAKSGLDVFAHNI 242

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 243 ETVEELQNFVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 302

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 303 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQKLGMEMGFRYVASGPMVRSSYKAGE 362

Query: 361 FYIKSMIESDRAA 374
           FYIKSMIESDRAA
Sbjct: 363 FYIKSMIESDRAA 374

BLAST of MELO3C018756T1 vs. TrEMBL
Match: A0A0R0HEM4_SOYBN (Lipoyl synthase, mitochondrial OS=Glycine max GN=LIP1 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 1.2e-186
Identity = 327/379 (86.28%), Postives = 352/379 (92.88%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R F SSS++T  +A   QFP+TLAGLR RLA ESPSLSDF+ L+
Sbjct: 3   MHSRVRSVAGNIKCAARLFCSSSTTTPPAAPL-QFPQTLAGLRARLAEESPSLSDFLALK 62

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 63  SESAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 122

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 123 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 182

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKPNMLIEALVPDFRGD+ CVEKVA+SGLDVFAHNI
Sbjct: 183 SVDRDDLPDQGSGHFTETVQKLKALKPNMLIEALVPDFRGDADCVEKVAKSGLDVFAHNI 242

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE +P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 243 ETVEELQSVVRDHRANFKQSLDVLMMAKENAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 302

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 303 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQKLGMEMGFRYVASGPMVRSSYKAGE 362

Query: 361 FYIKSMIESDRAASNSQPT 380
           FYIKSMIESDRAAS S+ T
Sbjct: 363 FYIKSMIESDRAASPSKLT 380

BLAST of MELO3C018756T1 vs. TrEMBL
Match: A0A0S3SH95_PHAAN (Lipoyl synthase, mitochondrial OS=Vigna angularis var. angularis GN=Vigan.07G094500 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.4e-186
Identity = 325/379 (85.75%), Postives = 353/379 (93.14%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R FSSSS+++ A+   SQF  TLAGLR+RLA ESPSLSDF+ L+
Sbjct: 3   MHSRIRSVAGNIKCAARLFSSSSATSPAAP--SQFSNTLAGLRDRLAEESPSLSDFIALK 62

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 63  SESAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 122

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 123 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 182

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLKVLKPN+LIEALVPDFRGD  CVEKVA+SGLDVFAHNI
Sbjct: 183 SVDRDDLPDQGSGHFTETVQKLKVLKPNILIEALVPDFRGDDDCVEKVAKSGLDVFAHNI 242

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRA+FKQSL VL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 243 ETVEELQNVVRDHRASFKQSLKVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 302

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 303 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQTLGMEMGFRYVASGPMVRSSYKAGE 362

Query: 361 FYIKSMIESDRAASNSQPT 380
           FYIKSMIESDRAAS S+ T
Sbjct: 363 FYIKSMIESDRAASPSKLT 379

BLAST of MELO3C018756T1 vs. TAIR10
Match: AT2G20860.1 (AT2G20860.1 lipoic acid synthase 1)

HSP 1 Score: 633.3 bits (1632), Expect = 1.0e-181
Identity = 311/376 (82.71%), Postives = 338/376 (89.89%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESPSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFIH-- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  -GDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV++SGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL MAKE++P+GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNS 377
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

BLAST of MELO3C018756T1 vs. TAIR10
Match: AT5G08415.1 (AT5G08415.1 Radical SAM superfamily protein)

HSP 1 Score: 381.3 bits (978), Expect = 6.8e-106
Identity = 176/299 (58.86%), Postives = 230/299 (76.92%), Query Frame = 1

Query: 78  KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGD 137
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 138 TCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE 197
           TCTRGCRFC VKTSR PPPPDP EP N A+AIASWG+DY+VITSVDRDD+PD GSGHFA+
Sbjct: 157 TCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQ 216

Query: 198 TVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNIETVEELQGAVRDHRANF 257
           TV+ +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD RA +
Sbjct: 217 TVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGY 276

Query: 258 KQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSK 317
           +QS+ VL  AK  S  G +TKTSIMLG GET +++ + M  +RA  VD++T GQY++P+ 
Sbjct: 277 EQSMSVLKHAK-ISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTP 336

Query: 318 RHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNS 377
            H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   + S S
Sbjct: 337 LHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKSLS 394

BLAST of MELO3C018756T1 vs. NCBI nr
Match: gi|659110655|ref|XP_008455341.1| (PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 762.7 bits (1968), Expect = 3.1e-217
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of MELO3C018756T1 vs. NCBI nr
Match: gi|449438074|ref|XP_004136815.1| (PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 753.1 bits (1943), Expect = 2.5e-214
Identity = 376/381 (98.69%), Postives = 378/381 (99.21%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQG VRDHRANFKQSLDVLVMAKEFS SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 361 FYIKSMIESDRAASNSQPTQL 382
           FYIKSMIESDRAASNSQPTQL
Sbjct: 361 FYIKSMIESDRAASNSQPTQL 381

BLAST of MELO3C018756T1 vs. NCBI nr
Match: gi|502082920|ref|XP_004487312.1| (PREDICTED: lipoyl synthase 2, mitochondrial [Cicer arietinum])

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-187
Identity = 325/376 (86.44%), Postives = 356/376 (94.68%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M  R +++   LK  ++ FSSSSS+T  +AT+S+FP+ LAGLR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVTGNLKCATKRFSSSSSTT--TATASEFPQNLAGLRARLAVESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGL+YVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLEYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK+LKP+MLIEALVPDFRG++ CVEKV++SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKILKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ AVRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASNS 377
           FYIKSMI+SDRAAS+S
Sbjct: 362 FYIKSMIDSDRAASSS 375

BLAST of MELO3C018756T1 vs. NCBI nr
Match: gi|1012323735|gb|KYP35828.1| (hypothetical protein KK1_043119 [Cajanus cajan])

HSP 1 Score: 663.7 bits (1711), Expect = 2.0e-187
Identity = 326/377 (86.47%), Postives = 353/377 (93.63%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R F SSS++T A+   SQFP+TLAGLR RLA ESPSL+DF+ L+
Sbjct: 2   MHSRFRSVAGNIKCAARLFCSSSTTTPAAP--SQFPQTLAGLRARLAEESPSLTDFIALK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 62  SESAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF ETVQKLK LKPNMLIEALVPDFRGD+ CVEKVA+SGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPNMLIEALVPDFRGDADCVEKVAKSGLDVFAHNI 241

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 242 ETVEELQNVVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 301

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 361 FYIKSMIESDRAASNSQ 378
           FYIKSMIESDRAAS S+
Sbjct: 362 FYIKSMIESDRAASPSK 376

BLAST of MELO3C018756T1 vs. NCBI nr
Match: gi|356549944|ref|XP_003543350.1| (PREDICTED: lipoyl synthase 2, mitochondrial [Glycine max])

HSP 1 Score: 662.1 bits (1707), Expect = 5.7e-187
Identity = 327/373 (87.67%), Postives = 350/373 (93.83%), Query Frame = 1

Query: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60
           M+ R +S+A  +K  +R F SSS+ T A+A S Q P+TLAGLR RLA ESPSLSDFV L+
Sbjct: 3   MHSRIRSVAGNIKCAARLFCSSSTITPAAAPS-QLPQTLAGLRARLAEESPSLSDFVALK 62

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S  +YSVEVGTKKKPLPKPKWMKE+VPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 63  SDSAYSVEVGTKKKPLPKPKWMKEAVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 122

Query: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 123 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 182

Query: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240
           SVDRDDLPDQGSGHF+ETVQKLK LKPNMLIEALVPDFRGD+ CVEKVA+SGLDVFAHNI
Sbjct: 183 SVDRDDLPDQGSGHFSETVQKLKALKPNMLIEALVPDFRGDADCVEKVAKSGLDVFAHNI 242

Query: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300
           ETVEELQ  VRDHRANFKQSLDVL+MAKE++P+GTLTKTSIMLGCGETPDQVV+TMEKVR
Sbjct: 243 ETVEELQNFVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQVVKTMEKVR 302

Query: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360
           AAGVDVMTFGQYMRPSKRHMPVSEY+TPEAF+KYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 303 AAGVDVMTFGQYMRPSKRHMPVSEYVTPEAFDKYQKLGMEMGFRYVASGPMVRSSYKAGE 362

Query: 361 FYIKSMIESDRAA 374
           FYIKSMIESDRAA
Sbjct: 363 FYIKSMIESDRAA 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
LIAS2_PEA1.1e-18584.27Lipoyl synthase 2, mitochondrial OS=Pisum sativum GN=LIP1-2 PE=2 SV=1[more]
LIAS1_PEA1.4e-18584.27Lipoyl synthase 1, mitochondrial OS=Pisum sativum GN=LIP1-1 PE=2 SV=1[more]
LIAS_POPTR7.0e-18586.34Lipoyl synthase, mitochondrial OS=Populus trichocarpa GN=LIP1 PE=3 SV=1[more]
LIAS_MEDTR1.3e-18382.67Lipoyl synthase, mitochondrial OS=Medicago truncatula GN=LIP1 PE=2 SV=1[more]
LIAS_ARATH1.8e-18082.71Lipoyl synthase, mitochondrial OS=Arabidopsis thaliana GN=LIP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K1R3_CUCSA1.7e-21498.69Lipoyl synthase, mitochondrial OS=Cucumis sativus GN=LIP1 PE=3 SV=1[more]
A0A151QZP0_CAJCA1.4e-18786.47Uncharacterized protein OS=Cajanus cajan GN=KK1_043119 PE=3 SV=1[more]
A0A0R4J536_SOYBN4.0e-18787.67Lipoyl synthase, mitochondrial OS=Glycine max GN=LIP1 PE=3 SV=1[more]
A0A0R0HEM4_SOYBN1.2e-18686.28Lipoyl synthase, mitochondrial OS=Glycine max GN=LIP1 PE=3 SV=1[more]
A0A0S3SH95_PHAAN3.4e-18685.75Lipoyl synthase, mitochondrial OS=Vigna angularis var. angularis GN=Vigan.07G094... [more]
Match NameE-valueIdentityDescription
AT2G20860.11.0e-18182.71 lipoic acid synthase 1[more]
AT5G08415.16.8e-10658.86 Radical SAM superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659110655|ref|XP_008455341.1|3.1e-217100.00PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis melo][more]
gi|449438074|ref|XP_004136815.1|2.5e-21498.69PREDICTED: lipoyl synthase 2, mitochondrial [Cucumis sativus][more]
gi|502082920|ref|XP_004487312.1|1.1e-18786.44PREDICTED: lipoyl synthase 2, mitochondrial [Cicer arietinum][more]
gi|1012323735|gb|KYP35828.1|2.0e-18786.47hypothetical protein KK1_043119 [Cajanus cajan][more]
gi|356549944|ref|XP_003543350.1|5.7e-18787.67PREDICTED: lipoyl synthase 2, mitochondrial [Glycine max][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR003698Lipoyl_synth
IPR006638Elp3/MiaB/NifB
IPR007197rSAM
IPR013785Aldolase_TIM
IPR027527Lipoyl_synth_mt
Vocabulary: Molecular Function
TermDefinition
GO:00515394 iron, 4 sulfur cluster binding
GO:0016992lipoate synthase activity
GO:0003824catalytic activity
GO:0051536iron-sulfur cluster binding
Vocabulary: Biological Process
TermDefinition
GO:0009107lipoate biosynthetic process
Vocabulary: Cellular Component
TermDefinition
GO:0005739mitochondrion
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009249 protein lipoylation
biological_process GO:0009107 lipoate biosynthetic process
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0046872 metal ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C018756MELO3C018756gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C018756T1MELO3C018756T1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C018756T1.cds4MELO3C018756T1.cds4CDS
MELO3C018756T1.cds3MELO3C018756T1.cds3CDS
MELO3C018756T1.cds2MELO3C018756T1.cds2CDS
MELO3C018756T1.cds1MELO3C018756T1.cds1CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 78..372
score: 132
IPR003698Lipoyl synthasePIRPIRSF005963Lipoyl_synthcoord: 15..380
score: 4.1E
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 7..380
score: 1.4E
IPR003698Lipoyl synthaseTIGRFAMsTIGR00510TIGR00510coord: 72..366
score: 1.3E
IPR006638Elongator protein 3/MiaB/NifBSMARTSM00729MiaBcoord: 129..336
score: 2.
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 136..298
score: 4.5
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 94..360
score: 1.3
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 20..375
score: 62
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 7..380
score: 1.4E
NoneNo IPR availableunknownSSF102114Radical SAM enzymescoord: 94..369
score: 8.5