BLAST of MELO3C012343T1 vs. Swiss-Prot
Match:
CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)
HSP 1 Score: 795.8 bits (2054), Expect = 3.8e-229
Identity = 397/697 (56.96%), Postives = 505/697 (72.45%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG + S HH +L++ +GS+F P+S+LH++KRSFNGF K+TEEEA+K++ MEG
Sbjct: 38 MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
V+SVF N +LHTTRSWDF+GF V R VES+I+VGV DTGIWPESPSFDD G+ P
Sbjct: 98 VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PP KWKG+CE S NF CN KIIGARSYH GD+ GPRD++GHGTHTAST AGGLV
Sbjct: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
QAN+ GLG GTARGGVP ARIA YKVCW+DGC+D DILAA+DDAIADGVDIIS S+G
Sbjct: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGA 277
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
+YF D++AIGSFHA+++GIL+S +AGN GP F T A+ SPW L+VAAST DR F T
Sbjct: 278 NPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQ 337
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
V++G+G+ +G SINTFD + PLV G DIP S SR C + SV+ L KGKIV
Sbjct: 338 VQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIV 397
Query: 361 VCDTVIDATEVV-AVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
VC+ E ++ GA G++M ++ SYP+P+S +D L YI S S P
Sbjct: 398 VCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRS-P 457
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
ATI KST AP V SFSSRGPN T +++KPD+S PGVEILAAWP + +P G +
Sbjct: 458 GATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV-APVGGIR 517
Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
N L+NIISGTSM+CPH+T +A YVK+++PTWSPAA+KSALMTTA PM+ + N + EF
Sbjct: 518 RN--TLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEF 577
Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
AYG+GH+NPL AV PGL+YDA+E+DYV+FLCGQGY T+ ++ ++ D + C+S ++ V+D
Sbjct: 578 AYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWD 637
Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
LNYPSF L+ + S+ NQ + RT+T+V +++TY+A I P + L I VNP+VLSFN LG
Sbjct: 638 LNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAP-QGLTISVNPNVLSFNGLG 697
Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITV 697
+ +SF +T++G I + SASLVW DG H VRSPIT+
Sbjct: 698 DRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727
BLAST of MELO3C012343T1 vs. Swiss-Prot
Match:
SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 681.8 bits (1758), Expect = 7.9e-195
Identity = 356/702 (50.71%), Postives = 470/702 (66.95%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG P+ K S PSHH+ +L++ +G+ L+ S+KRSFNGF A +++ E++K+ M+
Sbjct: 37 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
V+SVFP+ +L TTRSWDF+GF E+ +R ESD+IVGV D+GIWPES SFDD G+GP
Sbjct: 97 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PP KWKGSC+ F+CNNK+IGAR Y+ RD +GHGTHTAST AG V
Sbjct: 157 PPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAV 216
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
Q A+ GL GTARGGVPSARIA YKVC+ + C D DILAAFDDAIADGVD+IS S+ A
Sbjct: 217 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 276
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
N N S+AIGSFHAM +GI+++ +AGN GP ++AN SPW +TVAAS TDR F
Sbjct: 277 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 336
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
V LG+G+ L G S+NTF++ G + P+VYG ++ + N S + + C VD EL KGKIV
Sbjct: 337 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 396
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
+CD + E + GA+G+I+Q+ P P PAS + + I SYI S P
Sbjct: 397 LCDDFLGYREAY-LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-P 456
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV- 480
A I ++ E + AP V SFSSRGP+ V N+LKPD+SAPG+EILAA+ P++SPS +
Sbjct: 457 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 516
Query: 481 -EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
ED + V Y+++SGTSMACPHV VAAYVKSFHP WSP+A+KSA+MTTA PM+ K+N E+
Sbjct: 517 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 576
Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
EFAYG+G +NP A PGL+Y+ DY++ LC +G+ + L S N TCS + V
Sbjct: 577 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEV 636
Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
DLNYP+ ++ +P N ++RTVTNVG ++TYKA+++ L+I + P +L F
Sbjct: 637 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 696
Query: 661 LGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
L E++SF VTI GK + D S+S+VW DG H VRSPI +
Sbjct: 697 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 725
BLAST of MELO3C012343T1 vs. Swiss-Prot
Match:
SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 657.9 bits (1696), Expect = 1.2e-187
Identity = 341/706 (48.30%), Postives = 470/706 (66.57%), Query Frame = 1
Query: 1 MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
+G+ P + TP S HM +L+E G S L+ S+K+SFNGF A++TE E K+++ ME
Sbjct: 39 LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98
Query: 61 GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
V+SVFP+ K +L TT SW+FMG E +K R ++ESD I+GV D+GI+PES SF D G
Sbjct: 99 RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158
Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
+GPPP KWKG+C NF+CNNK+IGAR Y + + + RD GHGTHTAS AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218
Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
V +N GLG GTARGGVP+ARIAVYKVC ++GC +++AFDDAIADGVD+IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278
Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
+ D +AIG+FHAM G+L+ AAGN GP T+ + +PW +VAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338
Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
V LGDG+ L G S+NT+DM G PLVYG + S +R C +D +L KG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398
Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
KIV+CD+ E + GAVG I+++ P P S + + ++SY+NST +
Sbjct: 399 KIVLCDSTKGLIEAQKL-GAVGSIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKN 458
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
P AT+ KS E +RAP VASFSSRGP+ + +ILKPD++APGVEILAA+ P SSP+ +
Sbjct: 459 -PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTES 518
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
D +RV Y+++SGTSMACPHV VAAYVK+FHP WSP+ ++SA+MTTA+PM+ +
Sbjct: 519 EFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFV 578
Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
EFAYG+GH++P+ A++PGL+Y+ ++ D++ FLCG YT++ L+++S DN+TC+ S
Sbjct: 579 STEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISK 638
Query: 601 TV-FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIIN-PWKNLEIKVNPSVL 660
T+ +LNYP+ + + ++P N ++RTVTNVG + +TY A ++ P L IKV+P VL
Sbjct: 639 TLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVL 698
Query: 661 SFNHLGEEQSFEVTIKGKIMNDIE--SASLVWDDGKHKVRSPITVF 698
S + E+QSF VT+ + + SA+L+W DG H VRSPI V+
Sbjct: 699 SMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of MELO3C012343T1 vs. Swiss-Prot
Match:
SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 636.7 bits (1641), Expect = 2.9e-181
Identity = 332/693 (47.91%), Postives = 454/693 (65.51%), Query Frame = 1
Query: 13 SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
S HM +L++ G S L+ S+KRSFNGF A++TE E ++E+EGV+SVFPN QL
Sbjct: 50 SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109
Query: 73 HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
HTT SWDFMG E KR A+ESD I+GV DTGIWPES SF D G+GPPP KWKG C
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169
Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
NF+CNNK+IGAR Y S +G RD+ GHGTHTAST AG V+ + G+G
Sbjct: 170 GGKNFTCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229
Query: 193 GTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAPGFVNYFNDSM 252
GT RGGVP++RIA YKVC GC+ +L++FDDAIADGVD+I+ S+G + +D +
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289
Query: 253 AIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRELK 312
AIG+FHAM KGIL+ +AGN+GP T+++ +PW TVAASTT+R F T V LG+G+ L
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349
Query: 313 GASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDATE 372
G S+N FDMKGK+ PLVYG + + + C ++ KGKI+VC +
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGP-SGYK 409
Query: 373 VVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTERK 432
+ GA+ II + P ++ +PAS + +K ++SYI S +S P A + K+
Sbjct: 410 IAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTETIF 469
Query: 433 RKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNIIS 492
+ +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P PS +D +RV Y++ S
Sbjct: 470 NRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFS 529
Query: 493 GTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--KEKEFAYGAGHLNP 552
GTSMACPHV VAAYVK+F+P WSP+ ++SA+MTTA+P+ K EFAYGAGH++P
Sbjct: 530 GTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDP 589
Query: 553 LGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDLNYPSFALA 612
+ A++PGL+Y+ + D++ FLCG YT++ L+++S D CS + +LNYPS +
Sbjct: 590 MAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAK 649
Query: 613 TNASEPINQV-YRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVLSFNHLGEEQSFEV 672
+ ++ V + RT+TNVG+ ++TYK+ ++ L IKV PSVL F + E+QSF V
Sbjct: 650 LSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSV 709
Query: 673 TIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
T+ G ++ SA+L+W DG H VRSPI V+
Sbjct: 710 TVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of MELO3C012343T1 vs. Swiss-Prot
Match:
SBT4D_ARATH (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 636.0 bits (1639), Expect = 5.0e-181
Identity = 333/706 (47.17%), Postives = 463/706 (65.58%), Query Frame = 1
Query: 1 MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
MG+ TP S HM +L+E G S L+ S+KRSFNGF A++TE E ++V++M
Sbjct: 36 MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95
Query: 61 GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
GV+SVFPN K QL TT SWDFMG E +K R P VESD I+GV D+GI PES SF D G
Sbjct: 96 GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155
Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
+GPPP KWKG C NF+CNNK+IGAR Y S +G RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS---------EGTRDMDGHGTHTASTAAG 215
Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
V A+ G+G GT RGGVP++R+A YKVC GC+ +L+AFDDAIADGVD+I+ S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275
Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
G + ND +AIG+FHAM KG+L+ +AGN+GP +++ +PW LTVAASTT+R F
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335
Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
T V LG+G+ L G S+N ++MKGK PLVYG + + + C + VD KG
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKG 395
Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
KI+VC ++V GAVG+I + P +P+PA+ + ++ ++SY+ ST+S
Sbjct: 396 KILVCGGP-GGLKIVESVGAVGLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDS 455
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
P A + K+ + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P PS
Sbjct: 456 -PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS-- 515
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
+D + V Y+++SGTSM+CPHV VAAYVK+F+P WSP+ ++SA+MTTA+P++
Sbjct: 516 QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIA 575
Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
EFAYG+GH++P+ A +PGL+Y+ ++D++ FLCG YT+++L+++S + TCS
Sbjct: 576 STEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI 635
Query: 601 TVFDLNYPSF-ALATNASEPINQVYRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVL 660
+LNYPS A + + + RT+TNVG+ ++TY + ++ L++K+ PSVL
Sbjct: 636 LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVL 695
Query: 661 SFNHLGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
SF + E+QSF VT+ G ++ SA+L+W DG H VRSPI V+
Sbjct: 696 SFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728
BLAST of MELO3C012343T1 vs. TrEMBL
Match:
A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)
HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 612/699 (87.55%), Postives = 655/699 (93.71%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 37 MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 96
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 97 VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 156
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSYHSSG EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 216
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
+QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+ P
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 276
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 277 GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 336
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS S +C+ NSVDL+LAKGKIV
Sbjct: 337 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIV 396
Query: 361 VCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNSI 420
+CD + + E VAVKGAVGIIMQ+DSP D S+PIPASH+D+KSGA+ILSYINSTNSI
Sbjct: 397 MCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSI 456
Query: 421 PTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV 480
PTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SPSGAV
Sbjct: 457 PTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAV 516
Query: 481 EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKE 540
EDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN++KE
Sbjct: 517 EDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKE 576
Query: 541 FAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF 600
FAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDSDTVF
Sbjct: 577 FAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVF 636
Query: 601 DLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHL 660
DLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLSF L
Sbjct: 637 DLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLSFTSL 696
Query: 661 GEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
GE+QSFEVTI+GKI +IESASLVW+DGKHKVRSPITVF
Sbjct: 697 GEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 735
BLAST of MELO3C012343T1 vs. TrEMBL
Match:
A0A0A0KWL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1)
HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/698 (84.38%), Postives = 635/698 (90.97%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38 MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD GYG
Sbjct: 98 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSY S+G+ EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
G +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRE G S+NTFD+KGKQIPLVY GDIPKA SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
VCD++ VVAVKGAVGIIMQDDS D +S+PIPASH+ K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517
Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577
Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637
Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697
Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
E+QSFEVTI+GKI DIESASLVWDDGKHKVRSPITVF
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVF 735
BLAST of MELO3C012343T1 vs. TrEMBL
Match:
A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)
HSP 1 Score: 939.9 bits (2428), Expect = 1.8e-270
Identity = 477/703 (67.85%), Postives = 561/703 (79.80%), Query Frame = 1
Query: 1 MGNHPK--GKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEM 60
MG+H + P + HHMR+L+E +GS+F P LLHS+KRSFNGFVAK+TE EAKKVSEM
Sbjct: 36 MGSHHQVSSAPLSSHHHMRILQEAVGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEM 95
Query: 61 EGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGY 120
EGVISVFPNG+ QLHTTRSWDFMG SEQV+RVP+VESDIIVGVFDTGIWPESPSF D GY
Sbjct: 96 EGVISVFPNGELQLHTTRSWDFMGMSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 155
Query: 121 GPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGG 180
GPPP KWKGSCEVSANFSCNNKIIGARSY S G+ DIKGPRDS+GHGTH ASTVAGG
Sbjct: 156 GPPPPKWKGSCEVSANFSCNNKIIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 215
Query: 181 LVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLG 240
LV+QA+MLGLG GTARGGVPSARIA YKVCWSD C+DAD+LAAFDDAIADGVDIIS S+G
Sbjct: 216 LVRQASMLGLGMGTARGGVPSARIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVG 275
Query: 241 AP-GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
NYF D +AIG+FHAM+ GIL+S +AGN GP T+ N+SPW+L+VAAST+DR F
Sbjct: 276 PKRPRPNYFQDPIAIGTFHAMRNGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRF 335
Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLELA 360
T V+LGDGR+ G +INTFD+ G Q PLVY G+IP + S SR C+ +SVD EL
Sbjct: 336 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELV 395
Query: 361 KGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINS 420
KGKI +CD+ + ++V +++ AVGIIMQD SP D ++P+PASH+ + +I SY+NS
Sbjct: 396 KGKIAICDSFVSPSDVGSLESAVGIIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNS 455
Query: 421 TNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSP 480
T IPTATI KST K + AP VASFSSRGPNP + ILKPD+ PGVEILAAW P+ SP
Sbjct: 456 TR-IPTATILKSTGLKLQVAPLVASFSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSP 515
Query: 481 SGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 540
S A DN+++L+NIISGTSMACPH TAVAAYVKSFHP+WSPAALKSAL+TTAFPM
Sbjct: 516 SNAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLY 575
Query: 541 KEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDS 600
E EFAYG+GH+NPLGAV+PGLIY+ASETDY+RFLC +GY T L+++++DN+TCS+ S
Sbjct: 576 PEAEFAYGSGHINPLGAVNPGLIYNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQS 635
Query: 601 DTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLS 660
V+DLNYPSFAL T+ S P +Q +R VTNVGS ++TYKATI P L I VNPS+LS
Sbjct: 636 IRVYDLNYPSFALFTHISTPFSQTSKRRVTNVGSTNSTYKATISAP-SGLNITVNPSILS 695
Query: 661 FNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
F L EE +FEVT +GKI IESASLVWDDG HKVRSPI VF
Sbjct: 696 FKALEEELNFEVTFEGKIDRSIESASLVWDDGVHKVRSPIIVF 736
BLAST of MELO3C012343T1 vs. TrEMBL
Match:
A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)
HSP 1 Score: 896.7 bits (2316), Expect = 1.7e-257
Identity = 443/700 (63.29%), Postives = 538/700 (76.86%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGN + STPSHHMR+L+E +GSSF P++LLHS+KRSFNGFV K+TEEEA+K+S E
Sbjct: 1 MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
V+SVFPN KK LHTTRSWDFMGF+++ RV VES+I+VGV D+GIWPESPSF D GYGP
Sbjct: 61 VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PP KWKG+C+ SANF CN KIIGAR+Y S DIK PRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
QA++ GL GTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIIS S+G
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
YFNDS+AIG+FH+MK GIL+S +AGN GP + TI N+SPWSL+VAAS+ DR +
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIP--KANISSSLSRECVENSVDLELAKGK 360
V+LG+ +G +INTFD+KGKQ PL+Y G P A + S SR C NSVD L KGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
Query: 361 IVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNS 420
IV+CD+V+ V++ GAVG++M D D SYP+P+S++D G I +Y++ T
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTR- 420
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
PTATI KS AP + SFSSRGPNP T +ILKPDL+APGVEILAAW PI++ S
Sbjct: 421 FPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSG 480
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
V D++ LYNIISGTSM+CPH TA A YVK+FHPTWSPAA+KSALMTTA P++ K N +
Sbjct: 481 VRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQV 540
Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
EFAYGAGH+NPL AVHPGL+YDA E+DYVRFLCGQGYTT +++ +S DN+ C+ +S V
Sbjct: 541 EFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV 600
Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
+DLNYPSFAL++ +S+ NQ +RRTVTNVGSK +TY+A ++ + L I VNP VLSFN
Sbjct: 601 WDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNA 660
Query: 661 LGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
+G+++SF +TI+G I I SASLVW DG H VRSPITVF
Sbjct: 661 IGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVF 699
BLAST of MELO3C012343T1 vs. TrEMBL
Match:
A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)
HSP 1 Score: 866.7 bits (2238), Expect = 1.9e-248
Identity = 428/701 (61.06%), Postives = 534/701 (76.18%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGN P+ STPSHHMR+L+E GS+F P+SLLHS+KRSFNGFV K+TEEEA ++S EG
Sbjct: 40 MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
V+SVFP+GKK LHTTRSWDF+GF++ V RV VESDI+VGV D+GIWPE+PSF D GYGP
Sbjct: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PAKWKG C+ NF+CN KIIGAR+Y S DI PRDS+GHGTHTASTVAGGLV
Sbjct: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
QA++ GL GTARGGVPSARIAVYK+CWSDGC+DADILAAFDDAIADGVDIIS S+G
Sbjct: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
YFNDS+AIG+FH+MK GIL+S +AGN GP + TI N+SPWSL+VAASTTDR +
Sbjct: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLELAKGK 360
VE+G+ +G +INTFD GKQ PL+Y GD P + S+SR C E SVD L GK
Sbjct: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399
Query: 361 IVVCDTVIDATEVVAVKGAVGIIMQDDS-PMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
I++CD+++ + V AVG++M DD +SYP+P+S++++ G I +Y+ ++N
Sbjct: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYM-ASNG 459
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
+PTATI KS AP + SFSSRGPNP TL+ILKPDL+APGVEILAAW PI+ S
Sbjct: 460 VPTATIFKSDAVNDSSAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSG 519
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
V D++ LYNIISGTSM+CPHVTA A YVK+FHPTWSPAA+KSALMTTA P+ P+ N E
Sbjct: 520 VIDSRTTLYNIISGTSMSCPHVTAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEA 579
Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
EFAYGAG +NPL A+ PGL+YDA+E DYV+FLCGQGYT++++Q +S DN C+S + V
Sbjct: 580 EFAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRV 639
Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
+DLNYPSFAL++ S+ INQ + RT+T+V S ++TY +TI+ + L I VNP VLSF+
Sbjct: 640 WDLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSG 699
Query: 661 LGEEQSFEVTIKGKI-MNDIESASLVWDDGKHKVRSPITVF 698
+GE+++F +TI+G I I SASLVW D H VRSPIT++
Sbjct: 700 IGEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIY 739
BLAST of MELO3C012343T1 vs. TAIR10
Match:
AT5G59190.1 (AT5G59190.1 subtilase family protein)
HSP 1 Score: 681.8 bits (1758), Expect = 4.5e-196
Identity = 356/702 (50.71%), Postives = 470/702 (66.95%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG P+ K S PSHH+ +L++ +G+ L+ S+KRSFNGF A +++ E++K+ M+
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
V+SVFP+ +L TTRSWDF+GF E+ +R ESD+IVGV D+GIWPES SFDD G+GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PP KWKGSC+ F+CNNK+IGAR Y+ RD +GHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAV 180
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
Q A+ GL GTARGGVPSARIA YKVC+ + C D DILAAFDDAIADGVD+IS S+ A
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
N N S+AIGSFHAM +GI+++ +AGN GP ++AN SPW +TVAAS TDR F
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
V LG+G+ L G S+NTF++ G + P+VYG ++ + N S + + C VD EL KGKIV
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 360
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
+CD + E + GA+G+I+Q+ P P PAS + + I SYI S P
Sbjct: 361 LCDDFLGYREAY-LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-P 420
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV- 480
A I ++ E + AP V SFSSRGP+ V N+LKPD+SAPG+EILAA+ P++SPS +
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480
Query: 481 -EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
ED + V Y+++SGTSMACPHV VAAYVKSFHP WSP+A+KSA+MTTA PM+ K+N E+
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540
Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
EFAYG+G +NP A PGL+Y+ DY++ LC +G+ + L S N TCS + V
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEV 600
Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
DLNYP+ ++ +P N ++RTVTNVG ++TYKA+++ L+I + P +L F
Sbjct: 601 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 660
Query: 661 LGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
L E++SF VTI GK + D S+S+VW DG H VRSPI +
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689
BLAST of MELO3C012343T1 vs. TAIR10
Match:
AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)
HSP 1 Score: 657.9 bits (1696), Expect = 6.9e-189
Identity = 341/706 (48.30%), Postives = 470/706 (66.57%), Query Frame = 1
Query: 1 MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
+G+ P + TP S HM +L+E G S L+ S+K+SFNGF A++TE E K+++ ME
Sbjct: 39 LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98
Query: 61 GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
V+SVFP+ K +L TT SW+FMG E +K R ++ESD I+GV D+GI+PES SF D G
Sbjct: 99 RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158
Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
+GPPP KWKG+C NF+CNNK+IGAR Y + + + RD GHGTHTAS AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218
Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
V +N GLG GTARGGVP+ARIAVYKVC ++GC +++AFDDAIADGVD+IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278
Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
+ D +AIG+FHAM G+L+ AAGN GP T+ + +PW +VAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338
Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
V LGDG+ L G S+NT+DM G PLVYG + S +R C +D +L KG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398
Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
KIV+CD+ E + GAVG I+++ P P S + + ++SY+NST +
Sbjct: 399 KIVLCDSTKGLIEAQKL-GAVGSIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKN 458
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
P AT+ KS E +RAP VASFSSRGP+ + +ILKPD++APGVEILAA+ P SSP+ +
Sbjct: 459 -PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTES 518
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
D +RV Y+++SGTSMACPHV VAAYVK+FHP WSP+ ++SA+MTTA+PM+ +
Sbjct: 519 EFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFV 578
Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
EFAYG+GH++P+ A++PGL+Y+ ++ D++ FLCG YT++ L+++S DN+TC+ S
Sbjct: 579 STEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISK 638
Query: 601 TV-FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIIN-PWKNLEIKVNPSVL 660
T+ +LNYP+ + + ++P N ++RTVTNVG + +TY A ++ P L IKV+P VL
Sbjct: 639 TLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVL 698
Query: 661 SFNHLGEEQSFEVTIKGKIMNDIE--SASLVWDDGKHKVRSPITVF 698
S + E+QSF VT+ + + SA+L+W DG H VRSPI V+
Sbjct: 699 SMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738
BLAST of MELO3C012343T1 vs. TAIR10
Match:
AT5G59090.1 (AT5G59090.1 subtilase 4.12)
HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-182
Identity = 332/693 (47.91%), Postives = 454/693 (65.51%), Query Frame = 1
Query: 13 SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
S HM +L++ G S L+ S+KRSFNGF A++TE E ++E+EGV+SVFPN QL
Sbjct: 50 SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109
Query: 73 HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
HTT SWDFMG E KR A+ESD I+GV DTGIWPES SF D G+GPPP KWKG C
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169
Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
NF+CNNK+IGAR Y S +G RD+ GHGTHTAST AG V+ + G+G
Sbjct: 170 GGKNFTCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229
Query: 193 GTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAPGFVNYFNDSM 252
GT RGGVP++RIA YKVC GC+ +L++FDDAIADGVD+I+ S+G + +D +
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289
Query: 253 AIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRELK 312
AIG+FHAM KGIL+ +AGN+GP T+++ +PW TVAASTT+R F T V LG+G+ L
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349
Query: 313 GASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDATE 372
G S+N FDMKGK+ PLVYG + + + C ++ KGKI+VC +
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGP-SGYK 409
Query: 373 VVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTERK 432
+ GA+ II + P ++ +PAS + +K ++SYI S +S P A + K+
Sbjct: 410 IAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTETIF 469
Query: 433 RKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNIIS 492
+ +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P PS +D +RV Y++ S
Sbjct: 470 NRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFS 529
Query: 493 GTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--KEKEFAYGAGHLNP 552
GTSMACPHV VAAYVK+F+P WSP+ ++SA+MTTA+P+ K EFAYGAGH++P
Sbjct: 530 GTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDP 589
Query: 553 LGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDLNYPSFALA 612
+ A++PGL+Y+ + D++ FLCG YT++ L+++S D CS + +LNYPS +
Sbjct: 590 MAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAK 649
Query: 613 TNASEPINQV-YRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVLSFNHLGEEQSFEV 672
+ ++ V + RT+TNVG+ ++TYK+ ++ L IKV PSVL F + E+QSF V
Sbjct: 650 LSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSV 709
Query: 673 TIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
T+ G ++ SA+L+W DG H VRSPI V+
Sbjct: 710 TVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729
BLAST of MELO3C012343T1 vs. TAIR10
Match:
AT5G59120.1 (AT5G59120.1 subtilase 4.13)
HSP 1 Score: 636.0 bits (1639), Expect = 2.8e-182
Identity = 333/706 (47.17%), Postives = 463/706 (65.58%), Query Frame = 1
Query: 1 MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
MG+ TP S HM +L+E G S L+ S+KRSFNGF A++TE E ++V++M
Sbjct: 36 MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95
Query: 61 GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
GV+SVFPN K QL TT SWDFMG E +K R P VESD I+GV D+GI PES SF D G
Sbjct: 96 GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155
Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
+GPPP KWKG C NF+CNNK+IGAR Y S +G RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS---------EGTRDMDGHGTHTASTAAG 215
Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
V A+ G+G GT RGGVP++R+A YKVC GC+ +L+AFDDAIADGVD+I+ S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275
Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
G + ND +AIG+FHAM KG+L+ +AGN+GP +++ +PW LTVAASTT+R F
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335
Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
T V LG+G+ L G S+N ++MKGK PLVYG + + + C + VD KG
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKG 395
Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
KI+VC ++V GAVG+I + P +P+PA+ + ++ ++SY+ ST+S
Sbjct: 396 KILVCGGP-GGLKIVESVGAVGLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDS 455
Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
P A + K+ + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P PS
Sbjct: 456 -PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS-- 515
Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
+D + V Y+++SGTSM+CPHV VAAYVK+F+P WSP+ ++SA+MTTA+P++
Sbjct: 516 QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIA 575
Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
EFAYG+GH++P+ A +PGL+Y+ ++D++ FLCG YT+++L+++S + TCS
Sbjct: 576 STEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI 635
Query: 601 TVFDLNYPSF-ALATNASEPINQVYRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVL 660
+LNYPS A + + + RT+TNVG+ ++TY + ++ L++K+ PSVL
Sbjct: 636 LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVL 695
Query: 661 SFNHLGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
SF + E+QSF VT+ G ++ SA+L+W DG H VRSPI V+
Sbjct: 696 SFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728
BLAST of MELO3C012343T1 vs. TAIR10
Match:
AT3G46850.1 (AT3G46850.1 Subtilase family protein)
HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-180
Identity = 335/695 (48.20%), Postives = 458/695 (65.90%), Query Frame = 1
Query: 13 SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
SHH +L++ G S L+ ++KRSFNGF A++TE E + ++ M+ V+SVFP+ L
Sbjct: 51 SHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNL 110
Query: 73 HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
TT SW+FMG E + KR P +ESD I+GV D+GI+PES SF G+GPPP KWKG C+
Sbjct: 111 QTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCK 170
Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
NF+CNNK+IGAR Y LEG + RD+ GHG+HTAS AG V+ + GLG
Sbjct: 171 GGTNFTCNNKLIGARYYTPK---LEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGN 230
Query: 193 GTARGGVPSARIAVYKVCWSD--GCADADILAAFDDAIADGVDIISGSLGAPGFVNYFND 252
GT RGGVP+ARIAVYKVC C ILAAFDDAIAD VDII+ SLGA + D
Sbjct: 231 GTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEED 290
Query: 253 SMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRE 312
++AIG+FHAM KGIL+ AGN GP RTI + +PW TVAAS +R F T V LG+G+
Sbjct: 291 TLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKT 350
Query: 313 LKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDA 372
+ G S+N+FD+ GK+ PLVYG + +S + C +D + KGKIV+CDT +
Sbjct: 351 IVGRSVNSFDLNGKKYPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNP 410
Query: 373 TEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTE 432
E A+ GAV I+++ + P S + ++LSY+NST + P A + KS
Sbjct: 411 GEAQAM-GAVASIVRNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKN-PKAAVLKSET 470
Query: 433 RKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNI 492
++AP VAS+SSRGPNP+ +ILKPD++APG EILAA+ P PS + D + V Y +
Sbjct: 471 IFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTV 530
Query: 493 ISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPK---RNKEKEFAYGAGH 552
ISGTSM+CPHV VAAY+K+FHP WSP+ ++SA+MTTA+PM+ N+ EFAYGAGH
Sbjct: 531 ISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGH 590
Query: 553 LNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF-DLNYPS 612
++P+ A+HPGL+Y+A+++D++ FLCG YT + L+L+S D+++C+ + ++ +LNYPS
Sbjct: 591 VDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPS 650
Query: 613 FALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGEEQSF 672
+ + ++P +RRTVTNVG +ATYKA ++ L++KV P+VLS L E++SF
Sbjct: 651 MSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSF 710
Query: 673 EVTIK--GKIMNDIESASLVWDDGKHKVRSPITVF 698
VT+ G ++ SA L+W DG H VRSPI V+
Sbjct: 711 TVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of MELO3C012343T1 vs. NCBI nr
Match:
gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 698/698 (100.00%), Postives = 698/698 (100.00%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG
Sbjct: 732 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 791
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 792 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 851
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV
Sbjct: 852 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 911
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP
Sbjct: 912 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 971
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM
Sbjct: 972 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 1031
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV
Sbjct: 1032 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 1091
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT 420
VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT
Sbjct: 1092 VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT 1151
Query: 421 ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED 480
ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED
Sbjct: 1152 ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED 1211
Query: 481 NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA 540
NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA
Sbjct: 1212 NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA 1271
Query: 541 YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL 600
YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL
Sbjct: 1272 YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL 1331
Query: 601 NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE 660
NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE
Sbjct: 1332 NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE 1391
Query: 661 EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF 699
EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF
Sbjct: 1392 EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF 1429
BLAST of MELO3C012343T1 vs. NCBI nr
Match:
gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 588/690 (85.22%), Postives = 629/690 (91.16%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG+HPKGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE EAKKVSEMEG
Sbjct: 37 MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD TGYGP
Sbjct: 97 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSY S+G+ EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
+QANMLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
GF +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRE G S+NTFD+KGKQIPLVY GDIPKA SS+SR C EN++DL+L KGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
VCD++ V AVKGAVGIIMQDD SP D +S+PIPASH+ ++GA+ILSYINSTNSIP
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW PIS PSGA E
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516
Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
D++RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAF MS K N +KEF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576
Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
YGAGH+NPLGAVHPGLIYDASE DYV FLCGQGYTTELLQ VSEDNNTCSSN+SDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636
Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
LNYPSFAL+TN S+PINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSF +LG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696
Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHK 690
EEQSFEVTIKGKI +IESASLVWDDGKHK
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHK 726
HSP 2 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 612/699 (87.55%), Postives = 655/699 (93.71%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 37 MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 96
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 97 VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 156
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSYHSSG EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 216
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
+QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+ P
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 276
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 277 GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 336
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS S +C+ NSVDL+LAKGKIV
Sbjct: 337 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIV 396
Query: 361 VCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNSI 420
+CD + + E VAVKGAVGIIMQ+DSP D S+PIPASH+D+KSGA+ILSYINSTNSI
Sbjct: 397 MCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSI 456
Query: 421 PTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV 480
PTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SPSGAV
Sbjct: 457 PTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAV 516
Query: 481 EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKE 540
EDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN++KE
Sbjct: 517 EDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKE 576
Query: 541 FAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF 600
FAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDSDTVF
Sbjct: 577 FAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVF 636
Query: 601 DLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHL 660
DLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLSF L
Sbjct: 637 DLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLSFTSL 696
Query: 661 GEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
GE+QSFEVTI+GKI +IESASLVW+DGKHKVRSPITVF
Sbjct: 697 GEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 735
BLAST of MELO3C012343T1 vs. NCBI nr
Match:
gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])
HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 613/703 (87.20%), Postives = 656/703 (93.31%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 733 MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 792
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 793 VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 852
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSYHSSG EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 853 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 912
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
+QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+ P
Sbjct: 913 RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 972
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 973 GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 1032
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSR----ECVENSVDLELAK 360
VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS SR +C+ NSVDL+LAK
Sbjct: 1033 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSRYFXTQCLRNSVDLKLAK 1092
Query: 361 GKIVVCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINS 420
GKIV+CD + + E VAVKGAVGIIMQ+DSP D S+PIPASH+D+KSGA+ILSYINS
Sbjct: 1093 GKIVMCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINS 1152
Query: 421 TNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSP 480
TNSIPTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SP
Sbjct: 1153 TNSIPTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASP 1212
Query: 481 SGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 540
SGAVEDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN
Sbjct: 1213 SGAVEDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 1272
Query: 541 KEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDS 600
++KEFAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDS
Sbjct: 1273 QDKEFAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDS 1332
Query: 601 DTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLS 660
DTVFDLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLS
Sbjct: 1333 DTVFDLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLS 1392
Query: 661 FNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
F LGE+QSFEVTI+GKI +IESASLVW+DGKHKVRSPITVF
Sbjct: 1393 FTSLGEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 1435
BLAST of MELO3C012343T1 vs. NCBI nr
Match:
gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])
HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 581/690 (84.20%), Postives = 627/690 (90.87%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38 MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD GYG
Sbjct: 98 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSY S+G+ EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
G +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRE G S+NTFD+KGKQIPLVY GDIPKA SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
VCD++ VVAVKGAVGIIMQDDS D +S+PIPASH+ K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517
Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577
Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637
Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697
Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHK 690
E+QSFEVTI+GKI DIESASLVWDDGKHK
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHK 727
HSP 2 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/698 (84.38%), Postives = 635/698 (90.97%), Query Frame = 1
Query: 1 MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38 MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97
Query: 61 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD GYG
Sbjct: 98 VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157
Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
PPAKWKGSCEVSANFSCNNKIIGARSY S+G+ EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217
Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277
Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
G +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337
Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
VELGDGRE G S+NTFD+KGKQIPLVY GDIPKA SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397
Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
VCD++ VVAVKGAVGIIMQDDS D +S+PIPASH+ K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457
Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517
Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577
Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637
Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697
Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
E+QSFEVTI+GKI DIESASLVWDDGKHKVRSPITVF
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVF 735
BLAST of MELO3C012343T1 vs. NCBI nr
Match:
gi|659074279|ref|XP_008437519.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])
HSP 1 Score: 948.7 bits (2451), Expect = 5.6e-273
Identity = 484/705 (68.65%), Postives = 566/705 (80.28%), Query Frame = 1
Query: 1 MGNHPKGK----PSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVS 60
MG+HPK + P + HHMRLL+E IGS+F P LLHS+KRSFNGFVAK+TE EAKKVS
Sbjct: 37 MGSHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVS 96
Query: 61 EMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDT 120
EMEGVISVFPN + QLHTTRSWDFMGFSEQV+RVP+VESDIIVGVFDTGIWPESPSF D
Sbjct: 97 EMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 156
Query: 121 GYGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVA 180
GYGPPP KWKGSCEVS NFSCNNKIIGA+SY S G+ DIKGPRDS+GHGTH ASTVA
Sbjct: 157 GYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 216
Query: 181 GGLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGS 240
GGLV+QA+MLGLG GTARGGVPSARIA YKVCWSD C+ AD+LAAFDDAIADGVDIIS S
Sbjct: 217 GGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLS 276
Query: 241 LGAPGFV-NYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDR 300
+G + NYFND +AIG+FHAM+ GIL+S +AGN GP T+ N+SPW+L+VAASTTDR
Sbjct: 277 VGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDR 336
Query: 301 VFETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLE 360
F T V+LGDGR+ G +INTFD+ G Q PLV+ G+IP + S+SR C+ NSVD E
Sbjct: 337 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRE 396
Query: 361 LAKGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYI 420
L KGKI VCD+++ ++V +++ A+GIIMQD SP D ++P+PASH+ ++ +I SY+
Sbjct: 397 LVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYL 456
Query: 421 NSTNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPIS 480
N T IPTATI KSTE K + AP VASFSSRGPNP T +ILKPD+ PGVEILAAW PI
Sbjct: 457 NLTR-IPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIR 516
Query: 481 SPSGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPK 540
SPS A DN+++L+NIISGTSMACPH TAVAAYVKSFHP+WSPAALKSAL+TTAFPM
Sbjct: 517 SPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGD 576
Query: 541 RNKEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSN 600
E EFAYG+GH+NPLGAV+PGLIY+ASE DY+RFLCG+GY T LL+++++DN+TCS
Sbjct: 577 LYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPT 636
Query: 601 DSDTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSV 660
+S V+DLNY SFAL T S P +QVY+R VTNVGS ++TYKATI P L I VNPS
Sbjct: 637 NSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKATIFAP-SELNITVNPST 696
Query: 661 LSFNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
LSF L EE FEVTI+GKI I SASLVWDDG HKVRSPI VF
Sbjct: 697 LSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVF 739
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
CUCM1_CUCME | 3.8e-229 | 56.96 | Cucumisin OS=Cucumis melo PE=1 SV=1 | [more] |
SBT43_ARATH | 7.9e-195 | 50.71 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1 | [more] |
SBT44_ARATH | 1.2e-187 | 48.30 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1 | [more] |
SBT4C_ARATH | 2.9e-181 | 47.91 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1 | [more] |
SBT4D_ARATH | 5.0e-181 | 47.17 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KRC4_CUCSA | 0.0e+00 | 87.55 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1 | [more] |
A0A0A0KWL9_CUCSA | 0.0e+00 | 84.38 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1 | [more] |
A0A0A0KJN5_CUCSA | 1.8e-270 | 67.85 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1 | [more] |
A0A0A0KLR4_CUCSA | 1.7e-257 | 63.29 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1 | [more] |
A0A0A0KLY4_CUCSA | 1.9e-248 | 61.06 | Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1 | [more] |