MELO3C012343T1 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C012343T1
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionSubtilisin-like serine protease
Locationchr10 : 1131577 .. 1134728 (+)
Sequence length2097
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAATCATCCAAAAGGGAAACCTTCAACACCATCTCACCATATGAGATTACTAAAAGAAACCATTGGCAGGTAATGATTAATGATGATCATCTAATACATAAAAAGGAATTCACAAGAAAATAAAAGCTTATTGACTATAATTAATTTTCTTTTTTTCTCAGTTCTTTCCCTCCAAAATCTTTACTCCATAGCTTCAAGAGAAGCTTCAATGGATTTGTGGCCAAGATGACTGAAGAAGAAGCAAAGAAAGTTTCAGGTAATAAATAGTATTTCAGATTAGGAGGCGAATTAGCGACAATGGAACTGATTACTATTTGTTTTTAACACATAAATGTTGCATTTCAGAGATGGAAGGGGTGATATCAGTTTTCCCAAATGGGAAAAAGCAACTGCACACAACAAGATCTTGGGACTTCATGGGCTTCTCGGAACAAGTGAAGAGAGTTCCGGCGGTGGAAAGTGATATCATTGTCGGAGTATTCGACACCGGGATTTGGCCGGAATCTCCTAGTTTTGACGACACGGGATACGGTCCGCCGCCGGCCAAGTGGAAGGGCAGCTGTGAAGTCTCTGCCAATTTCTCTTGCAACAAGTAATTCAACATATATATCTACAAAACTTTATTTAATGCATGGTTTGAATTTACTTTGTAAATGCTAAATAAAATACCACCTTTTTTGATCTTTTTTGTTCGACTTTTAATTTTTGAAAGTGTATATTTGACAGTAAAATCATTGGAGCTCGATCATATCATAGCAGCGGTCAGGACCTAGAAGGCGACATCAAAGGTCCAAGAGATTCAGATGGTCACGGGACACACACAGCATCGACGGTAGCGGGAGGGTTGGTTCAGCAAGCAAATATGTTGGGACTTGGCTTCGGCACAGCCAGAGGTGGAGTCCCATCAGCACGCATTGCCGTATACAAAGTATGCTGGTCCGACGGCTGTGCTGACGCCGACATCCTTGCTGCATTCGACGATGCCATTGCTGACGGCGTCGACATCATCTCTGGCTCTCTCGGAGCACCAGGGTTTGTAAATTACTTCAACGACTCCATGGCCATTGGATCTTTTCATGCAATGAAAAAGGGAATCTTGTCGTCATTAGCAGCTGGAAATACCGGCCCAGGTTTCAGAACCATTGCAAACTACTCGCCATGGTCGTTGACAGTAGCGGCTAGCACTACTGATCGGGTATTCGAAACTATGGTTGAGCTCGGAGATGGAAGAGAATTGAAAGTAAGAAATTTAATTTAATTTAACTCATTTTGTCATTTAAATTAAATCTATGAAACTAATTAGATAATTTCATTTTTGGTATATGAAGGGAGCCAGCATCAATACATTTGATATGAAGGGAAAACAAATTCCATTGGTTTACGGGGGAGATATTCCTAAAGCTAACATAAGCAGTTCCTTGTCGAGGTATTTTTAAACGTCTAAATTTAATTTTTATATATTCTTGGGCTGTAATAAATATTAAAAATGTTTGAGCTTTGAATCAGGGAGTGCGTTGAGAACTCAGTAGATTTGGAGTTGGCTAAAGGTAAAATTGTAGTGTGCGATACGGTGATCGATGCCACAGAAGTTGTGGCGGTAAAAGGTGCGGTGGGTATTATAATGCAAGATGATTCTCCAATGGATGGCCATTCTTATCCAATTCCTGCTTCTCATGTTGACTCTAAATCTGGTGCTGTCATTCTCTCTTACATCAACTCAACCAATAGGTAAACCTAATCAAAATTTTATAAATAATTAACCTTGTTTCCATTTCTATATATATATTCTAATTAAATCACTCCTGTGTATCCATAATGGTTAATTACATGATGGGTTTTACAGCATTCCGACAGCCACAATAAAGAAAAGCACAGAAAGAAAACGTAAAAGAGCTCCATCTGTTGCATCTTTTTCTTCAAGAGGTCCAAACCCAGTAACTCTCAACATTCTCAAGGTAACATATTTATTCAATTTTACTTTAATTTTATCGATGTTTTTTGGGATTAATTCTTTGTTTGTAGTTAATTATTAATTATATGACTTCTCTTATAAAAATTGCAGCCAGATTTGAGTGCTCCAGGAGTTGAAATTCTTGCAGCATGGCCTCCAATTTCTTCACCCTCAGGAGCTGTAGAGGATAACCAAAGAGTTCTTTATAATATCATCTCAGGAACTTCAATGGCTTGTCCACATGTTACTGCTGTAGCTGCTTATGTTAAATCATTCCATCCTACTTGGTCTCCTGCTGCCCTTAAATCTGCCCTCATGACTACAGGTAATTAGTTTCATGGTCTATTTTCAAATAAATGCATGGCAAAATATATCAAAACCCTGTTTATAATAAGCAGCATGCGATTGAGCAAGATAGATTATTGTGATATTTTGTTACATTTTTTATTTTATTTTTAATATTAACCAATCAAATCTATATGCACGCAGCGTTTCCAATGAGCCCTAAACGTAATAAAGAGAAAGAGTTTGCATATGGTGCTGGTCACTTAAATCCACTAGGCGCAGTGCATCCTGGATTAATCTACGATGCTTCTGAAACTGACTACGTACGGTTTTTATGTGGCCAAGGTACTGTTCGGAAAAATTTGAACGTATGAACAAAATTGTTGTTTTCTTTTTTCCCTAAAACAAAGACGACGTTGTTGGATATTTGATTTAATGGCTTTAGTTTAAAGGATGTGTTCTATTGTTAGACATTAATGATCATCGATCCCACACTATGTTCTTATACTCTTTTAATTTATTTTCCTACAGGTTATACCACCGAGTTGCTCCAACTAGTTTCGGAAGACAACAATACTTGTTCTTCGAACGATTCCGACACAGTGTTCGACCTAAACTATCCTTCATTCGCTCTTGCCACGAATGCCTCAGAACCAATCAACCAAGTTTATAGAAGAACTGTCACCAATGTTGGATCAAAATCTGCAACATATAAAGCTACTATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAATCCTTCTGTTCTTTCGTTCAATCATTTGGGAGAGGAGCAAAGTTTTGAGGTTACAATCAAGGGAAAAATTATGAATGATATTGAATCAGCTTCTTTGGTTTGGGATGATGGAAAACACAAAGTGAGGAGTCCTATAACAGTGTTTTTCTGA

mRNA sequence

ATGGGAAATCATCCAAAAGGGAAACCTTCAACACCATCTCACCATATGAGATTACTAAAAGAAACCATTGGCAGTTCTTTCCCTCCAAAATCTTTACTCCATAGCTTCAAGAGAAGCTTCAATGGATTTGTGGCCAAGATGACTGAAGAAGAAGCAAAGAAAGTTTCAGAGATGGAAGGGGTGATATCAGTTTTCCCAAATGGGAAAAAGCAACTGCACACAACAAGATCTTGGGACTTCATGGGCTTCTCGGAACAAGTGAAGAGAGTTCCGGCGGTGGAAAGTGATATCATTGTCGGAGTATTCGACACCGGGATTTGGCCGGAATCTCCTAGTTTTGACGACACGGGATACGGTCCGCCGCCGGCCAAGTGGAAGGGCAGCTGTGAAGTCTCTGCCAATTTCTCTTGCAACAATAAAATCATTGGAGCTCGATCATATCATAGCAGCGGTCAGGACCTAGAAGGCGACATCAAAGGTCCAAGAGATTCAGATGGTCACGGGACACACACAGCATCGACGGTAGCGGGAGGGTTGGTTCAGCAAGCAAATATGTTGGGACTTGGCTTCGGCACAGCCAGAGGTGGAGTCCCATCAGCACGCATTGCCGTATACAAAGTATGCTGGTCCGACGGCTGTGCTGACGCCGACATCCTTGCTGCATTCGACGATGCCATTGCTGACGGCGTCGACATCATCTCTGGCTCTCTCGGAGCACCAGGGTTTGTAAATTACTTCAACGACTCCATGGCCATTGGATCTTTTCATGCAATGAAAAAGGGAATCTTGTCGTCATTAGCAGCTGGAAATACCGGCCCAGGTTTCAGAACCATTGCAAACTACTCGCCATGGTCGTTGACAGTAGCGGCTAGCACTACTGATCGGGTATTCGAAACTATGGTTGAGCTCGGAGATGGAAGAGAATTGAAAGGAGCCAGCATCAATACATTTGATATGAAGGGAAAACAAATTCCATTGGTTTACGGGGGAGATATTCCTAAAGCTAACATAAGCAGTTCCTTGTCGAGGGAGTGCGTTGAGAACTCAGTAGATTTGGAGTTGGCTAAAGGTAAAATTGTAGTGTGCGATACGGTGATCGATGCCACAGAAGTTGTGGCGGTAAAAGGTGCGGTGGGTATTATAATGCAAGATGATTCTCCAATGGATGGCCATTCTTATCCAATTCCTGCTTCTCATGTTGACTCTAAATCTGGTGCTGTCATTCTCTCTTACATCAACTCAACCAATAGCATTCCGACAGCCACAATAAAGAAAAGCACAGAAAGAAAACGTAAAAGAGCTCCATCTGTTGCATCTTTTTCTTCAAGAGGTCCAAACCCAGTAACTCTCAACATTCTCAAGCCAGATTTGAGTGCTCCAGGAGTTGAAATTCTTGCAGCATGGCCTCCAATTTCTTCACCCTCAGGAGCTGTAGAGGATAACCAAAGAGTTCTTTATAATATCATCTCAGGAACTTCAATGGCTTGTCCACATGTTACTGCTGTAGCTGCTTATGTTAAATCATTCCATCCTACTTGGTCTCCTGCTGCCCTTAAATCTGCCCTCATGACTACAGCGTTTCCAATGAGCCCTAAACGTAATAAAGAGAAAGAGTTTGCATATGGTGCTGGTCACTTAAATCCACTAGGCGCAGTGCATCCTGGATTAATCTACGATGCTTCTGAAACTGACTACGTACGGTTTTTATGTGGCCAAGGTTATACCACCGAGTTGCTCCAACTAGTTTCGGAAGACAACAATACTTGTTCTTCGAACGATTCCGACACAGTGTTCGACCTAAACTATCCTTCATTCGCTCTTGCCACGAATGCCTCAGAACCAATCAACCAAGTTTATAGAAGAACTGTCACCAATGTTGGATCAAAATCTGCAACATATAAAGCTACTATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAATCCTTCTGTTCTTTCGTTCAATCATTTGGGAGAGGAGCAAAGTTTTGAGGTTACAATCAAGGGAAAAATTATGAATGATATTGAATCAGCTTCTTTGGTTTGGGATGATGGAAAACACAAAGTGAGGAGTCCTATAACAGTGTTTTTCTGA

Coding sequence (CDS)

ATGGGAAATCATCCAAAAGGGAAACCTTCAACACCATCTCACCATATGAGATTACTAAAAGAAACCATTGGCAGTTCTTTCCCTCCAAAATCTTTACTCCATAGCTTCAAGAGAAGCTTCAATGGATTTGTGGCCAAGATGACTGAAGAAGAAGCAAAGAAAGTTTCAGAGATGGAAGGGGTGATATCAGTTTTCCCAAATGGGAAAAAGCAACTGCACACAACAAGATCTTGGGACTTCATGGGCTTCTCGGAACAAGTGAAGAGAGTTCCGGCGGTGGAAAGTGATATCATTGTCGGAGTATTCGACACCGGGATTTGGCCGGAATCTCCTAGTTTTGACGACACGGGATACGGTCCGCCGCCGGCCAAGTGGAAGGGCAGCTGTGAAGTCTCTGCCAATTTCTCTTGCAACAATAAAATCATTGGAGCTCGATCATATCATAGCAGCGGTCAGGACCTAGAAGGCGACATCAAAGGTCCAAGAGATTCAGATGGTCACGGGACACACACAGCATCGACGGTAGCGGGAGGGTTGGTTCAGCAAGCAAATATGTTGGGACTTGGCTTCGGCACAGCCAGAGGTGGAGTCCCATCAGCACGCATTGCCGTATACAAAGTATGCTGGTCCGACGGCTGTGCTGACGCCGACATCCTTGCTGCATTCGACGATGCCATTGCTGACGGCGTCGACATCATCTCTGGCTCTCTCGGAGCACCAGGGTTTGTAAATTACTTCAACGACTCCATGGCCATTGGATCTTTTCATGCAATGAAAAAGGGAATCTTGTCGTCATTAGCAGCTGGAAATACCGGCCCAGGTTTCAGAACCATTGCAAACTACTCGCCATGGTCGTTGACAGTAGCGGCTAGCACTACTGATCGGGTATTCGAAACTATGGTTGAGCTCGGAGATGGAAGAGAATTGAAAGGAGCCAGCATCAATACATTTGATATGAAGGGAAAACAAATTCCATTGGTTTACGGGGGAGATATTCCTAAAGCTAACATAAGCAGTTCCTTGTCGAGGGAGTGCGTTGAGAACTCAGTAGATTTGGAGTTGGCTAAAGGTAAAATTGTAGTGTGCGATACGGTGATCGATGCCACAGAAGTTGTGGCGGTAAAAGGTGCGGTGGGTATTATAATGCAAGATGATTCTCCAATGGATGGCCATTCTTATCCAATTCCTGCTTCTCATGTTGACTCTAAATCTGGTGCTGTCATTCTCTCTTACATCAACTCAACCAATAGCATTCCGACAGCCACAATAAAGAAAAGCACAGAAAGAAAACGTAAAAGAGCTCCATCTGTTGCATCTTTTTCTTCAAGAGGTCCAAACCCAGTAACTCTCAACATTCTCAAGCCAGATTTGAGTGCTCCAGGAGTTGAAATTCTTGCAGCATGGCCTCCAATTTCTTCACCCTCAGGAGCTGTAGAGGATAACCAAAGAGTTCTTTATAATATCATCTCAGGAACTTCAATGGCTTGTCCACATGTTACTGCTGTAGCTGCTTATGTTAAATCATTCCATCCTACTTGGTCTCCTGCTGCCCTTAAATCTGCCCTCATGACTACAGCGTTTCCAATGAGCCCTAAACGTAATAAAGAGAAAGAGTTTGCATATGGTGCTGGTCACTTAAATCCACTAGGCGCAGTGCATCCTGGATTAATCTACGATGCTTCTGAAACTGACTACGTACGGTTTTTATGTGGCCAAGGTTATACCACCGAGTTGCTCCAACTAGTTTCGGAAGACAACAATACTTGTTCTTCGAACGATTCCGACACAGTGTTCGACCTAAACTATCCTTCATTCGCTCTTGCCACGAATGCCTCAGAACCAATCAACCAAGTTTATAGAAGAACTGTCACCAATGTTGGATCAAAATCTGCAACATATAAAGCTACTATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAATCCTTCTGTTCTTTCGTTCAATCATTTGGGAGAGGAGCAAAGTTTTGAGGTTACAATCAAGGGAAAAATTATGAATGATATTGAATCAGCTTCTTTGGTTTGGGATGATGGAAAACACAAAGTGAGGAGTCCTATAACAGTGTTTTTCTGA

Protein sequence

MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF*
BLAST of MELO3C012343T1 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.8e-229
Identity = 397/697 (56.96%), Postives = 505/697 (72.45%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG   +   S   HH  +L++ +GS+F P+S+LH++KRSFNGF  K+TEEEA+K++ MEG
Sbjct: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           V+SVF N   +LHTTRSWDF+GF   V R   VES+I+VGV DTGIWPESPSFDD G+ P
Sbjct: 98  VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PP KWKG+CE S NF CN KIIGARSYH       GD+ GPRD++GHGTHTAST AGGLV
Sbjct: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
            QAN+ GLG GTARGGVP ARIA YKVCW+DGC+D DILAA+DDAIADGVDIIS S+G  
Sbjct: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGA 277

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
              +YF D++AIGSFHA+++GIL+S +AGN GP F T A+ SPW L+VAAST DR F T 
Sbjct: 278 NPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQ 337

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           V++G+G+  +G SINTFD   +  PLV G DIP      S SR C + SV+  L KGKIV
Sbjct: 338 VQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIV 397

Query: 361 VCDTVIDATEVV-AVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
           VC+      E   ++ GA G++M  ++     SYP+P+S +D       L YI S  S P
Sbjct: 398 VCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRS-P 457

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
            ATI KST      AP V SFSSRGPN  T +++KPD+S PGVEILAAWP + +P G + 
Sbjct: 458 GATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSV-APVGGIR 517

Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
            N   L+NIISGTSM+CPH+T +A YVK+++PTWSPAA+KSALMTTA PM+ + N + EF
Sbjct: 518 RN--TLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEF 577

Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
           AYG+GH+NPL AV PGL+YDA+E+DYV+FLCGQGY T+ ++ ++ D + C+S ++  V+D
Sbjct: 578 AYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWD 637

Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
           LNYPSF L+ + S+  NQ + RT+T+V  +++TY+A I  P + L I VNP+VLSFN LG
Sbjct: 638 LNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAP-QGLTISVNPNVLSFNGLG 697

Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITV 697
           + +SF +T++G I   + SASLVW DG H VRSPIT+
Sbjct: 698 DRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727

BLAST of MELO3C012343T1 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 7.9e-195
Identity = 356/702 (50.71%), Postives = 470/702 (66.95%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG  P+ K S PSHH+ +L++ +G+      L+ S+KRSFNGF A +++ E++K+  M+ 
Sbjct: 37  MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           V+SVFP+   +L TTRSWDF+GF E+ +R    ESD+IVGV D+GIWPES SFDD G+GP
Sbjct: 97  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PP KWKGSC+    F+CNNK+IGAR Y+             RD +GHGTHTAST AG  V
Sbjct: 157 PPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAV 216

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           Q A+  GL  GTARGGVPSARIA YKVC+ + C D DILAAFDDAIADGVD+IS S+ A 
Sbjct: 217 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 276

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
              N  N S+AIGSFHAM +GI+++ +AGN GP   ++AN SPW +TVAAS TDR F   
Sbjct: 277 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 336

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           V LG+G+ L G S+NTF++ G + P+VYG ++ + N S + +  C    VD EL KGKIV
Sbjct: 337 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 396

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
           +CD  +   E   + GA+G+I+Q+   P      P PAS +  +    I SYI S    P
Sbjct: 397 LCDDFLGYREAY-LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-P 456

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV- 480
            A I ++ E   + AP V SFSSRGP+ V  N+LKPD+SAPG+EILAA+ P++SPS  + 
Sbjct: 457 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 516

Query: 481 -EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
            ED + V Y+++SGTSMACPHV  VAAYVKSFHP WSP+A+KSA+MTTA PM+ K+N E+
Sbjct: 517 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 576

Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
           EFAYG+G +NP  A  PGL+Y+    DY++ LC +G+ +  L   S  N TCS  +   V
Sbjct: 577 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEV 636

Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
            DLNYP+     ++ +P N  ++RTVTNVG  ++TYKA+++     L+I + P +L F  
Sbjct: 637 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 696

Query: 661 LGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           L E++SF VTI GK + D    S+S+VW DG H VRSPI  +
Sbjct: 697 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 725

BLAST of MELO3C012343T1 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 1.2e-187
Identity = 341/706 (48.30%), Postives = 470/706 (66.57%), Query Frame = 1

Query: 1   MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
           +G+ P  +  TP S HM +L+E  G S     L+ S+K+SFNGF A++TE E K+++ ME
Sbjct: 39  LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98

Query: 61  GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
            V+SVFP+ K +L TT SW+FMG  E +K  R  ++ESD I+GV D+GI+PES SF D G
Sbjct: 99  RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158

Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
           +GPPP KWKG+C    NF+CNNK+IGAR Y +  +  +      RD  GHGTHTAS  AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218

Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
             V  +N  GLG GTARGGVP+ARIAVYKVC ++GC    +++AFDDAIADGVD+IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278

Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
                  +  D +AIG+FHAM  G+L+  AAGN GP   T+ + +PW  +VAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338

Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
              V LGDG+ L G S+NT+DM G   PLVYG     +  S   +R C    +D +L KG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398

Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
           KIV+CD+     E   + GAVG I+++  P        P S + +     ++SY+NST +
Sbjct: 399 KIVLCDSTKGLIEAQKL-GAVGSIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKN 458

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
            P AT+ KS E   +RAP VASFSSRGP+ +  +ILKPD++APGVEILAA+ P SSP+ +
Sbjct: 459 -PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTES 518

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
             D +RV Y+++SGTSMACPHV  VAAYVK+FHP WSP+ ++SA+MTTA+PM+   +   
Sbjct: 519 EFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFV 578

Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
             EFAYG+GH++P+ A++PGL+Y+ ++ D++ FLCG  YT++ L+++S DN+TC+   S 
Sbjct: 579 STEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISK 638

Query: 601 TV-FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIIN-PWKNLEIKVNPSVL 660
           T+  +LNYP+ +   + ++P N  ++RTVTNVG + +TY A ++  P   L IKV+P VL
Sbjct: 639 TLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVL 698

Query: 661 SFNHLGEEQSFEVTIKGKIMNDIE--SASLVWDDGKHKVRSPITVF 698
           S   + E+QSF VT+    +   +  SA+L+W DG H VRSPI V+
Sbjct: 699 SMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of MELO3C012343T1 vs. Swiss-Prot
Match: SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.9e-181
Identity = 332/693 (47.91%), Postives = 454/693 (65.51%), Query Frame = 1

Query: 13  SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
           S HM +L++  G S     L+ S+KRSFNGF A++TE E   ++E+EGV+SVFPN   QL
Sbjct: 50  SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109

Query: 73  HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
           HTT SWDFMG  E    KR  A+ESD I+GV DTGIWPES SF D G+GPPP KWKG C 
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169

Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
              NF+CNNK+IGAR Y S         +G RD+ GHGTHTAST AG  V+  +  G+G 
Sbjct: 170 GGKNFTCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229

Query: 193 GTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAPGFVNYFNDSM 252
           GT RGGVP++RIA YKVC   GC+   +L++FDDAIADGVD+I+ S+G      + +D +
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289

Query: 253 AIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRELK 312
           AIG+FHAM KGIL+  +AGN+GP   T+++ +PW  TVAASTT+R F T V LG+G+ L 
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349

Query: 313 GASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDATE 372
           G S+N FDMKGK+ PLVYG     +   +  +  C    ++    KGKI+VC       +
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGP-SGYK 409

Query: 373 VVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTERK 432
           +    GA+ II +   P    ++ +PAS + +K    ++SYI S +S P A + K+    
Sbjct: 410 IAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTETIF 469

Query: 433 RKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNIIS 492
            + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P   PS   +D +RV Y++ S
Sbjct: 470 NRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFS 529

Query: 493 GTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--KEKEFAYGAGHLNP 552
           GTSMACPHV  VAAYVK+F+P WSP+ ++SA+MTTA+P+  K       EFAYGAGH++P
Sbjct: 530 GTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDP 589

Query: 553 LGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDLNYPSFALA 612
           + A++PGL+Y+  + D++ FLCG  YT++ L+++S D   CS  +     +LNYPS +  
Sbjct: 590 MAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAK 649

Query: 613 TNASEPINQV-YRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVLSFNHLGEEQSFEV 672
            + ++    V + RT+TNVG+ ++TYK+ ++      L IKV PSVL F  + E+QSF V
Sbjct: 650 LSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSV 709

Query: 673 TIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           T+ G  ++     SA+L+W DG H VRSPI V+
Sbjct: 710 TVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of MELO3C012343T1 vs. Swiss-Prot
Match: SBT4D_ARATH (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 5.0e-181
Identity = 333/706 (47.17%), Postives = 463/706 (65.58%), Query Frame = 1

Query: 1   MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
           MG+       TP S HM +L+E  G S     L+ S+KRSFNGF A++TE E ++V++M 
Sbjct: 36  MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95

Query: 61  GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
           GV+SVFPN K QL TT SWDFMG  E +K  R P VESD I+GV D+GI PES SF D G
Sbjct: 96  GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155

Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
           +GPPP KWKG C    NF+CNNK+IGAR Y S         +G RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS---------EGTRDMDGHGTHTASTAAG 215

Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
             V  A+  G+G GT RGGVP++R+A YKVC   GC+   +L+AFDDAIADGVD+I+ S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275

Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
           G      + ND +AIG+FHAM KG+L+  +AGN+GP   +++  +PW LTVAASTT+R F
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335

Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
            T V LG+G+ L G S+N ++MKGK  PLVYG     +   +  +  C  + VD    KG
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKG 395

Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
           KI+VC       ++V   GAVG+I +   P     +P+PA+ + ++    ++SY+ ST+S
Sbjct: 396 KILVCGGP-GGLKIVESVGAVGLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDS 455

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
            P A + K+     + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P   PS  
Sbjct: 456 -PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS-- 515

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
            +D + V Y+++SGTSM+CPHV  VAAYVK+F+P WSP+ ++SA+MTTA+P++       
Sbjct: 516 QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIA 575

Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
             EFAYG+GH++P+ A +PGL+Y+  ++D++ FLCG  YT+++L+++S +  TCS     
Sbjct: 576 STEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI 635

Query: 601 TVFDLNYPSF-ALATNASEPINQVYRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVL 660
              +LNYPS  A  + +       + RT+TNVG+ ++TY + ++      L++K+ PSVL
Sbjct: 636 LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVL 695

Query: 661 SFNHLGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           SF  + E+QSF VT+ G  ++     SA+L+W DG H VRSPI V+
Sbjct: 696 SFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of MELO3C012343T1 vs. TrEMBL
Match: A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 612/699 (87.55%), Postives = 655/699 (93.71%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 37  MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 96

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 156

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSYHSSG   EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 216

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           +QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+  P
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 276

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG  ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 277 GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 336

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS S +C+ NSVDL+LAKGKIV
Sbjct: 337 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIV 396

Query: 361 VCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNSI 420
           +CD +  +  E VAVKGAVGIIMQ+DSP D   S+PIPASH+D+KSGA+ILSYINSTNSI
Sbjct: 397 MCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSI 456

Query: 421 PTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV 480
           PTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SPSGAV
Sbjct: 457 PTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAV 516

Query: 481 EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKE 540
           EDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN++KE
Sbjct: 517 EDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKE 576

Query: 541 FAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF 600
           FAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDSDTVF
Sbjct: 577 FAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVF 636

Query: 601 DLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHL 660
           DLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLSF  L
Sbjct: 637 DLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLSFTSL 696

Query: 661 GEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           GE+QSFEVTI+GKI  +IESASLVW+DGKHKVRSPITVF
Sbjct: 697 GEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 735

BLAST of MELO3C012343T1 vs. TrEMBL
Match: A0A0A0KWL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1)

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/698 (84.38%), Postives = 635/698 (90.97%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD  GYG 
Sbjct: 98  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSY S+G+  EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           ++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG  
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           G  +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET 
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRE  G S+NTFD+KGKQIPLVY GDIPKA   SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
           VCD++     VVAVKGAVGIIMQDDS  D  +S+PIPASH+  K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
           TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
           DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
            YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
           LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           E+QSFEVTI+GKI  DIESASLVWDDGKHKVRSPITVF
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVF 735

BLAST of MELO3C012343T1 vs. TrEMBL
Match: A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 1.8e-270
Identity = 477/703 (67.85%), Postives = 561/703 (79.80%), Query Frame = 1

Query: 1   MGNHPK--GKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEM 60
           MG+H +    P +  HHMR+L+E +GS+F P  LLHS+KRSFNGFVAK+TE EAKKVSEM
Sbjct: 36  MGSHHQVSSAPLSSHHHMRILQEAVGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEM 95

Query: 61  EGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGY 120
           EGVISVFPNG+ QLHTTRSWDFMG SEQV+RVP+VESDIIVGVFDTGIWPESPSF D GY
Sbjct: 96  EGVISVFPNGELQLHTTRSWDFMGMSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGY 155

Query: 121 GPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGG 180
           GPPP KWKGSCEVSANFSCNNKIIGARSY S G+    DIKGPRDS+GHGTH ASTVAGG
Sbjct: 156 GPPPPKWKGSCEVSANFSCNNKIIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGG 215

Query: 181 LVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLG 240
           LV+QA+MLGLG GTARGGVPSARIA YKVCWSD C+DAD+LAAFDDAIADGVDIIS S+G
Sbjct: 216 LVRQASMLGLGMGTARGGVPSARIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVG 275

Query: 241 AP-GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
                 NYF D +AIG+FHAM+ GIL+S +AGN GP   T+ N+SPW+L+VAAST+DR F
Sbjct: 276 PKRPRPNYFQDPIAIGTFHAMRNGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRF 335

Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLELA 360
            T V+LGDGR+  G +INTFD+ G Q PLVY G+IP      + S SR C+ +SVD EL 
Sbjct: 336 LTAVQLGDGRKFNGVTINTFDLNGTQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELV 395

Query: 361 KGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINS 420
           KGKI +CD+ +  ++V +++ AVGIIMQD SP D   ++P+PASH+  +   +I SY+NS
Sbjct: 396 KGKIAICDSFVSPSDVGSLESAVGIIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNS 455

Query: 421 TNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSP 480
           T  IPTATI KST  K + AP VASFSSRGPNP +  ILKPD+  PGVEILAAW P+ SP
Sbjct: 456 TR-IPTATILKSTGLKLQVAPLVASFSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSP 515

Query: 481 SGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 540
           S A  DN+++L+NIISGTSMACPH TAVAAYVKSFHP+WSPAALKSAL+TTAFPM     
Sbjct: 516 SNAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLY 575

Query: 541 KEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDS 600
            E EFAYG+GH+NPLGAV+PGLIY+ASETDY+RFLC +GY T  L+++++DN+TCS+  S
Sbjct: 576 PEAEFAYGSGHINPLGAVNPGLIYNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQS 635

Query: 601 DTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLS 660
             V+DLNYPSFAL T+ S P +Q  +R VTNVGS ++TYKATI  P   L I VNPS+LS
Sbjct: 636 IRVYDLNYPSFALFTHISTPFSQTSKRRVTNVGSTNSTYKATISAP-SGLNITVNPSILS 695

Query: 661 FNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           F  L EE +FEVT +GKI   IESASLVWDDG HKVRSPI VF
Sbjct: 696 FKALEEELNFEVTFEGKIDRSIESASLVWDDGVHKVRSPIIVF 736

BLAST of MELO3C012343T1 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 1.7e-257
Identity = 443/700 (63.29%), Postives = 538/700 (76.86%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MGN  +   STPSHHMR+L+E +GSSF P++LLHS+KRSFNGFV K+TEEEA+K+S  E 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           V+SVFPN KK LHTTRSWDFMGF+++  RV  VES+I+VGV D+GIWPESPSF D GYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PP KWKG+C+ SANF CN KIIGAR+Y S       DIK PRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
            QA++ GL  GTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIIS S+G  
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
               YFNDS+AIG+FH+MK GIL+S +AGN GP + TI N+SPWSL+VAAS+ DR   + 
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIP--KANISSSLSRECVENSVDLELAKGK 360
           V+LG+    +G +INTFD+KGKQ PL+Y G  P   A  + S SR C  NSVD  L KGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 361 IVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNS 420
           IV+CD+V+     V++ GAVG++M D    D   SYP+P+S++D   G  I +Y++ T  
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTR- 420

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
            PTATI KS       AP + SFSSRGPNP T +ILKPDL+APGVEILAAW PI++ S  
Sbjct: 421 FPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSG 480

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
           V D++  LYNIISGTSM+CPH TA A YVK+FHPTWSPAA+KSALMTTA P++ K N + 
Sbjct: 481 VRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQV 540

Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
           EFAYGAGH+NPL AVHPGL+YDA E+DYVRFLCGQGYTT +++ +S DN+ C+  +S  V
Sbjct: 541 EFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV 600

Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
           +DLNYPSFAL++ +S+  NQ +RRTVTNVGSK +TY+A ++   + L I VNP VLSFN 
Sbjct: 601 WDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNA 660

Query: 661 LGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           +G+++SF +TI+G I   I SASLVW DG H VRSPITVF
Sbjct: 661 IGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVF 699

BLAST of MELO3C012343T1 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 1.9e-248
Identity = 428/701 (61.06%), Postives = 534/701 (76.18%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MGN P+   STPSHHMR+L+E  GS+F P+SLLHS+KRSFNGFV K+TEEEA ++S  EG
Sbjct: 40  MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           V+SVFP+GKK LHTTRSWDF+GF++ V RV  VESDI+VGV D+GIWPE+PSF D GYGP
Sbjct: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
            PAKWKG C+   NF+CN KIIGAR+Y S       DI  PRDS+GHGTHTASTVAGGLV
Sbjct: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
            QA++ GL  GTARGGVPSARIAVYK+CWSDGC+DADILAAFDDAIADGVDIIS S+G  
Sbjct: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
               YFNDS+AIG+FH+MK GIL+S +AGN GP + TI N+SPWSL+VAASTTDR   + 
Sbjct: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLELAKGK 360
           VE+G+    +G +INTFD  GKQ PL+Y GD P      + S+SR C E SVD  L  GK
Sbjct: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399

Query: 361 IVVCDTVIDATEVVAVKGAVGIIMQDDS-PMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
           I++CD+++  +  V    AVG++M DD      +SYP+P+S++++  G  I +Y+ ++N 
Sbjct: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYM-ASNG 459

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
           +PTATI KS       AP + SFSSRGPNP TL+ILKPDL+APGVEILAAW PI+  S  
Sbjct: 460 VPTATIFKSDAVNDSSAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSG 519

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
           V D++  LYNIISGTSM+CPHVTA A YVK+FHPTWSPAA+KSALMTTA P+ P+ N E 
Sbjct: 520 VIDSRTTLYNIISGTSMSCPHVTAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEA 579

Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
           EFAYGAG +NPL A+ PGL+YDA+E DYV+FLCGQGYT++++Q +S DN  C+S +   V
Sbjct: 580 EFAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRV 639

Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
           +DLNYPSFAL++  S+ INQ + RT+T+V S ++TY +TI+   + L I VNP VLSF+ 
Sbjct: 640 WDLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSG 699

Query: 661 LGEEQSFEVTIKGKI-MNDIESASLVWDDGKHKVRSPITVF 698
           +GE+++F +TI+G I    I SASLVW D  H VRSPIT++
Sbjct: 700 IGEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIY 739

BLAST of MELO3C012343T1 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 681.8 bits (1758), Expect = 4.5e-196
Identity = 356/702 (50.71%), Postives = 470/702 (66.95%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG  P+ K S PSHH+ +L++ +G+      L+ S+KRSFNGF A +++ E++K+  M+ 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           V+SVFP+   +L TTRSWDF+GF E+ +R    ESD+IVGV D+GIWPES SFDD G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PP KWKGSC+    F+CNNK+IGAR Y+             RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAV 180

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           Q A+  GL  GTARGGVPSARIA YKVC+ + C D DILAAFDDAIADGVD+IS S+ A 
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
              N  N S+AIGSFHAM +GI+++ +AGN GP   ++AN SPW +TVAAS TDR F   
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           V LG+G+ L G S+NTF++ G + P+VYG ++ + N S + +  C    VD EL KGKIV
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIV 360

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
           +CD  +   E   + GA+G+I+Q+   P      P PAS +  +    I SYI S    P
Sbjct: 361 LCDDFLGYREAY-LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP-P 420

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV- 480
            A I ++ E   + AP V SFSSRGP+ V  N+LKPD+SAPG+EILAA+ P++SPS  + 
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480

Query: 481 -EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEK 540
            ED + V Y+++SGTSMACPHV  VAAYVKSFHP WSP+A+KSA+MTTA PM+ K+N E+
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540

Query: 541 EFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTV 600
           EFAYG+G +NP  A  PGL+Y+    DY++ LC +G+ +  L   S  N TCS  +   V
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEV 600

Query: 601 FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNH 660
            DLNYP+     ++ +P N  ++RTVTNVG  ++TYKA+++     L+I + P +L F  
Sbjct: 601 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 660

Query: 661 LGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           L E++SF VTI GK + D    S+S+VW DG H VRSPI  +
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAY 689

BLAST of MELO3C012343T1 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 657.9 bits (1696), Expect = 6.9e-189
Identity = 341/706 (48.30%), Postives = 470/706 (66.57%), Query Frame = 1

Query: 1   MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
           +G+ P  +  TP S HM +L+E  G S     L+ S+K+SFNGF A++TE E K+++ ME
Sbjct: 39  LGSLPSREEYTPMSDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGME 98

Query: 61  GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
            V+SVFP+ K +L TT SW+FMG  E +K  R  ++ESD I+GV D+GI+PES SF D G
Sbjct: 99  RVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQG 158

Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
           +GPPP KWKG+C    NF+CNNK+IGAR Y +  +  +      RD  GHGTHTAS  AG
Sbjct: 159 FGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKANQT----ARDYSGHGTHTASIAAG 218

Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
             V  +N  GLG GTARGGVP+ARIAVYKVC ++GC    +++AFDDAIADGVD+IS S+
Sbjct: 219 NAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISI 278

Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
                  +  D +AIG+FHAM  G+L+  AAGN GP   T+ + +PW  +VAAS T+R F
Sbjct: 279 VLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAF 338

Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
              V LGDG+ L G S+NT+DM G   PLVYG     +  S   +R C    +D +L KG
Sbjct: 339 MAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKG 398

Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
           KIV+CD+     E   + GAVG I+++  P        P S + +     ++SY+NST +
Sbjct: 399 KIVLCDSTKGLIEAQKL-GAVGSIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKN 458

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
            P AT+ KS E   +RAP VASFSSRGP+ +  +ILKPD++APGVEILAA+ P SSP+ +
Sbjct: 459 -PKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTES 518

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
             D +RV Y+++SGTSMACPHV  VAAYVK+FHP WSP+ ++SA+MTTA+PM+   +   
Sbjct: 519 EFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFV 578

Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
             EFAYG+GH++P+ A++PGL+Y+ ++ D++ FLCG  YT++ L+++S DN+TC+   S 
Sbjct: 579 STEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISK 638

Query: 601 TV-FDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIIN-PWKNLEIKVNPSVL 660
           T+  +LNYP+ +   + ++P N  ++RTVTNVG + +TY A ++  P   L IKV+P VL
Sbjct: 639 TLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVL 698

Query: 661 SFNHLGEEQSFEVTIKGKIMNDIE--SASLVWDDGKHKVRSPITVF 698
           S   + E+QSF VT+    +   +  SA+L+W DG H VRSPI V+
Sbjct: 699 SMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

BLAST of MELO3C012343T1 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-182
Identity = 332/693 (47.91%), Postives = 454/693 (65.51%), Query Frame = 1

Query: 13  SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
           S HM +L++  G S     L+ S+KRSFNGF A++TE E   ++E+EGV+SVFPN   QL
Sbjct: 50  SDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQL 109

Query: 73  HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
           HTT SWDFMG  E    KR  A+ESD I+GV DTGIWPES SF D G+GPPP KWKG C 
Sbjct: 110 HTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCS 169

Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
              NF+CNNK+IGAR Y S         +G RD+ GHGTHTAST AG  V+  +  G+G 
Sbjct: 170 GGKNFTCNNKLIGARDYTS---------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGN 229

Query: 193 GTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAPGFVNYFNDSM 252
           GT RGGVP++RIA YKVC   GC+   +L++FDDAIADGVD+I+ S+G      + +D +
Sbjct: 230 GTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPI 289

Query: 253 AIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRELK 312
           AIG+FHAM KGIL+  +AGN+GP   T+++ +PW  TVAASTT+R F T V LG+G+ L 
Sbjct: 290 AIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLA 349

Query: 313 GASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDATE 372
           G S+N FDMKGK+ PLVYG     +   +  +  C    ++    KGKI+VC       +
Sbjct: 350 GRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGP-SGYK 409

Query: 373 VVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTERK 432
           +    GA+ II +   P    ++ +PAS + +K    ++SYI S +S P A + K+    
Sbjct: 410 IAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDS-PQAAVLKTETIF 469

Query: 433 RKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNIIS 492
            + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P   PS   +D +RV Y++ S
Sbjct: 470 NRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFS 529

Query: 493 GTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--KEKEFAYGAGHLNP 552
           GTSMACPHV  VAAYVK+F+P WSP+ ++SA+MTTA+P+  K       EFAYGAGH++P
Sbjct: 530 GTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDP 589

Query: 553 LGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDLNYPSFALA 612
           + A++PGL+Y+  + D++ FLCG  YT++ L+++S D   CS  +     +LNYPS +  
Sbjct: 590 MAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAK 649

Query: 613 TNASEPINQV-YRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVLSFNHLGEEQSFEV 672
            + ++    V + RT+TNVG+ ++TYK+ ++      L IKV PSVL F  + E+QSF V
Sbjct: 650 LSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSV 709

Query: 673 TIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           T+ G  ++     SA+L+W DG H VRSPI V+
Sbjct: 710 TVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of MELO3C012343T1 vs. TAIR10
Match: AT5G59120.1 (AT5G59120.1 subtilase 4.13)

HSP 1 Score: 636.0 bits (1639), Expect = 2.8e-182
Identity = 333/706 (47.17%), Postives = 463/706 (65.58%), Query Frame = 1

Query: 1   MGNHPKGKPSTP-SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEME 60
           MG+       TP S HM +L+E  G S     L+ S+KRSFNGF A++TE E ++V++M 
Sbjct: 36  MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95

Query: 61  GVISVFPNGKKQLHTTRSWDFMGFSEQVK--RVPAVESDIIVGVFDTGIWPESPSFDDTG 120
           GV+SVFPN K QL TT SWDFMG  E +K  R P VESD I+GV D+GI PES SF D G
Sbjct: 96  GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155

Query: 121 YGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAG 180
           +GPPP KWKG C    NF+CNNK+IGAR Y S         +G RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS---------EGTRDMDGHGTHTASTAAG 215

Query: 181 GLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSL 240
             V  A+  G+G GT RGGVP++R+A YKVC   GC+   +L+AFDDAIADGVD+I+ S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275

Query: 241 GAPGFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVF 300
           G      + ND +AIG+FHAM KG+L+  +AGN+GP   +++  +PW LTVAASTT+R F
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335

Query: 301 ETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKG 360
            T V LG+G+ L G S+N ++MKGK  PLVYG     +   +  +  C  + VD    KG
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKG 395

Query: 361 KIVVCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNS 420
           KI+VC       ++V   GAVG+I +   P     +P+PA+ + ++    ++SY+ ST+S
Sbjct: 396 KILVCGGP-GGLKIVESVGAVGLIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDS 455

Query: 421 IPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGA 480
            P A + K+     + +P +ASFSSRGPN + ++ILKPD++APGVEILAA+ P   PS  
Sbjct: 456 -PQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS-- 515

Query: 481 VEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN--K 540
            +D + V Y+++SGTSM+CPHV  VAAYVK+F+P WSP+ ++SA+MTTA+P++       
Sbjct: 516 QDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIA 575

Query: 541 EKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSD 600
             EFAYG+GH++P+ A +PGL+Y+  ++D++ FLCG  YT+++L+++S +  TCS     
Sbjct: 576 STEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKI 635

Query: 601 TVFDLNYPSF-ALATNASEPINQVYRRTVTNVGSKSATYKATII-NPWKNLEIKVNPSVL 660
              +LNYPS  A  + +       + RT+TNVG+ ++TY + ++      L++K+ PSVL
Sbjct: 636 LPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVL 695

Query: 661 SFNHLGEEQSFEVTIKGKIMND--IESASLVWDDGKHKVRSPITVF 698
           SF  + E+QSF VT+ G  ++     SA+L+W DG H VRSPI V+
Sbjct: 696 SFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of MELO3C012343T1 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-180
Identity = 335/695 (48.20%), Postives = 458/695 (65.90%), Query Frame = 1

Query: 13  SHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEGVISVFPNGKKQL 72
           SHH  +L++  G S     L+ ++KRSFNGF A++TE E + ++ M+ V+SVFP+    L
Sbjct: 51  SHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNL 110

Query: 73  HTTRSWDFMGFSE--QVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCE 132
            TT SW+FMG  E  + KR P +ESD I+GV D+GI+PES SF   G+GPPP KWKG C+
Sbjct: 111 QTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCK 170

Query: 133 VSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLVQQANMLGLGF 192
              NF+CNNK+IGAR Y      LEG  +  RD+ GHG+HTAS  AG  V+  +  GLG 
Sbjct: 171 GGTNFTCNNKLIGARYYTPK---LEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGN 230

Query: 193 GTARGGVPSARIAVYKVCWSD--GCADADILAAFDDAIADGVDIISGSLGAPGFVNYFND 252
           GT RGGVP+ARIAVYKVC      C    ILAAFDDAIAD VDII+ SLGA     +  D
Sbjct: 231 GTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEED 290

Query: 253 SMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETMVELGDGRE 312
           ++AIG+FHAM KGIL+   AGN GP  RTI + +PW  TVAAS  +R F T V LG+G+ 
Sbjct: 291 TLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKT 350

Query: 313 LKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIVVCDTVIDA 372
           + G S+N+FD+ GK+ PLVYG     +   +S +  C    +D +  KGKIV+CDT  + 
Sbjct: 351 IVGRSVNSFDLNGKKYPLVYGKS-ASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNP 410

Query: 373 TEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPTATIKKSTE 432
            E  A+ GAV  I+++        +  P S +      ++LSY+NST + P A + KS  
Sbjct: 411 GEAQAM-GAVASIVRNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKN-PKAAVLKSET 470

Query: 433 RKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVEDNQRVLYNI 492
              ++AP VAS+SSRGPNP+  +ILKPD++APG EILAA+ P   PS +  D + V Y +
Sbjct: 471 IFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTV 530

Query: 493 ISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPK---RNKEKEFAYGAGH 552
           ISGTSM+CPHV  VAAY+K+FHP WSP+ ++SA+MTTA+PM+      N+  EFAYGAGH
Sbjct: 531 ISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGH 590

Query: 553 LNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF-DLNYPS 612
           ++P+ A+HPGL+Y+A+++D++ FLCG  YT + L+L+S D+++C+   + ++  +LNYPS
Sbjct: 591 VDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPS 650

Query: 613 FALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGEEQSF 672
            +   + ++P    +RRTVTNVG  +ATYKA ++     L++KV P+VLS   L E++SF
Sbjct: 651 MSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKKSF 710

Query: 673 EVTIK--GKIMNDIESASLVWDDGKHKVRSPITVF 698
            VT+   G    ++ SA L+W DG H VRSPI V+
Sbjct: 711 TVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of MELO3C012343T1 vs. NCBI nr
Match: gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 698/698 (100.00%), Postives = 698/698 (100.00%), Query Frame = 1

Query: 1    MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
            MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG
Sbjct: 732  MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 791

Query: 61   VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
            VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 792  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 851

Query: 121  PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
            PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV
Sbjct: 852  PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 911

Query: 181  QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
            QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP
Sbjct: 912  QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 971

Query: 241  GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
            GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM
Sbjct: 972  GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 1031

Query: 301  VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
            VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV
Sbjct: 1032 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 1091

Query: 361  VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT 420
            VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT
Sbjct: 1092 VCDTVIDATEVVAVKGAVGIIMQDDSPMDGHSYPIPASHVDSKSGAVILSYINSTNSIPT 1151

Query: 421  ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED 480
            ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED
Sbjct: 1152 ATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVED 1211

Query: 481  NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA 540
            NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA
Sbjct: 1212 NQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEFA 1271

Query: 541  YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL 600
            YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL
Sbjct: 1272 YGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFDL 1331

Query: 601  NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE 660
            NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE
Sbjct: 1332 NYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLGE 1391

Query: 661  EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF 699
            EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF
Sbjct: 1392 EQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVFF 1429

BLAST of MELO3C012343T1 vs. NCBI nr
Match: gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 588/690 (85.22%), Postives = 629/690 (91.16%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG+HPKGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE EAKKVSEMEG
Sbjct: 37  MGSHPKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEG 96

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD TGYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGP 156

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSY S+G+  EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 216

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           +QANMLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG  
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 276

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           GF +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET 
Sbjct: 277 GFRDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 336

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRE  G S+NTFD+KGKQIPLVY GDIPKA   SS+SR C EN++DL+L KGKIV
Sbjct: 337 VELGDGREFNGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIV 396

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDD-SPMDGHSYPIPASHVDSKSGAVILSYINSTNSIP 420
           VCD++     V AVKGAVGIIMQDD SP D +S+PIPASH+  ++GA+ILSYINSTNSIP
Sbjct: 397 VCDSLTVPGGVAAVKGAVGIIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIP 456

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
           TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW PIS PSGA E
Sbjct: 457 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEE 516

Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
           D++RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAF MS K N +KEF
Sbjct: 517 DSKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEF 576

Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
            YGAGH+NPLGAVHPGLIYDASE DYV FLCGQGYTTELLQ VSEDNNTCSSN+SDTVFD
Sbjct: 577 GYGAGHINPLGAVHPGLIYDASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFD 636

Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
           LNYPSFAL+TN S+PINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSF +LG
Sbjct: 637 LNYPSFALSTNISKPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFKNLG 696

Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHK 690
           EEQSFEVTIKGKI  +IESASLVWDDGKHK
Sbjct: 697 EEQSFEVTIKGKIRKNIESASLVWDDGKHK 726


HSP 2 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 612/699 (87.55%), Postives = 655/699 (93.71%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 37  MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 96

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 97  VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 156

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSYHSSG   EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 157 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 216

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           +QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+  P
Sbjct: 217 RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 276

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG  ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 277 GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 336

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS S +C+ NSVDL+LAKGKIV
Sbjct: 337 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIV 396

Query: 361 VCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINSTNSI 420
           +CD +  +  E VAVKGAVGIIMQ+DSP D   S+PIPASH+D+KSGA+ILSYINSTNSI
Sbjct: 397 MCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSI 456

Query: 421 PTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAV 480
           PTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SPSGAV
Sbjct: 457 PTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAV 516

Query: 481 EDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKE 540
           EDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN++KE
Sbjct: 517 EDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKE 576

Query: 541 FAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVF 600
           FAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDSDTVF
Sbjct: 577 FAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVF 636

Query: 601 DLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHL 660
           DLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLSF  L
Sbjct: 637 DLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLSFTSL 696

Query: 661 GEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           GE+QSFEVTI+GKI  +IESASLVW+DGKHKVRSPITVF
Sbjct: 697 GEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 735

BLAST of MELO3C012343T1 vs. NCBI nr
Match: gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 613/703 (87.20%), Postives = 656/703 (93.31%), Query Frame = 1

Query: 1    MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
            MGNHPKGKPST SHHMRLLKE+IGSSFPP SLLHS+KRSFNGFVAKMTE+EAKKVSEMEG
Sbjct: 733  MGNHPKGKPSTSSHHMRLLKESIGSSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEG 792

Query: 61   VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
            VISVFPNGKKQLHTTRSW+FMGFSEQVKRVP VESDIIVGVFDTGIWPESPSFDDTGYGP
Sbjct: 793  VISVFPNGKKQLHTTRSWNFMGFSEQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGP 852

Query: 121  PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
            PPAKWKGSCEVSANFSCNNKIIGARSYHSSG   EGD++GP DS+GHGTHTASTVAGGLV
Sbjct: 853  PPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLV 912

Query: 181  QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
            +QANMLGLG GTARGGVPSARIAVYK+CWSD C+DADILAAFDDAIADGVDI+S S+  P
Sbjct: 913  RQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGP 972

Query: 241  GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
            GF NYFNDSMAIGSFHAMKKGILSS AAGNTGPG  ++ANYSPWSLTVAASTTDRV ET+
Sbjct: 973  GFKNYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETV 1032

Query: 301  VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSR----ECVENSVDLELAK 360
            VELGDGRELKG +INTFDMKGKQ+PLVYGGDIPKAN SSS SR    +C+ NSVDL+LAK
Sbjct: 1033 VELGDGRELKGVTINTFDMKGKQVPLVYGGDIPKANTSSSFSRYFXTQCLRNSVDLKLAK 1092

Query: 361  GKIVVCDTVIDA-TEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYINS 420
            GKIV+CD +  +  E VAVKGAVGIIMQ+DSP D   S+PIPASH+D+KSGA+ILSYINS
Sbjct: 1093 GKIVMCDMITTSPAEAVAVKGAVGIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINS 1152

Query: 421  TNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSP 480
            TNSIPTATIKKS ERKR+RAPSVASFSSRGPNPVT NILKPDLS PGVEILAAWPPI+SP
Sbjct: 1153 TNSIPTATIKKSIERKRRRAPSVASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASP 1212

Query: 481  SGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 540
            SGAVEDN+RVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN
Sbjct: 1213 SGAVEDNKRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRN 1272

Query: 541  KEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDS 600
            ++KEFAYGAGHLNPLGAVHPGLIYDASE DYVRFLCGQGYTTELLQLVS+ +NTCSSNDS
Sbjct: 1273 QDKEFAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNTCSSNDS 1332

Query: 601  DTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLS 660
            DTVFDLNYPSFAL+TN S PINQVYRRTVTN+GS+SA YKATIINPWKNL+IKVNPSVLS
Sbjct: 1333 DTVFDLNYPSFALSTNISVPINQVYRRTVTNIGSRSAMYKATIINPWKNLDIKVNPSVLS 1392

Query: 661  FNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
            F  LGE+QSFEVTI+GKI  +IESASLVW+DGKHKVRSPITVF
Sbjct: 1393 FTSLGEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVF 1435

BLAST of MELO3C012343T1 vs. NCBI nr
Match: gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 581/690 (84.20%), Postives = 627/690 (90.87%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD  GYG 
Sbjct: 98  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSY S+G+  EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           ++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG  
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           G  +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET 
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRE  G S+NTFD+KGKQIPLVY GDIPKA   SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
           VCD++     VVAVKGAVGIIMQDDS  D  +S+PIPASH+  K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
           TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
           DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
            YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
           LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHK 690
           E+QSFEVTI+GKI  DIESASLVWDDGKHK
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHK 727


HSP 2 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 589/698 (84.38%), Postives = 635/698 (90.97%), Query Frame = 1

Query: 1   MGNHPKGKPSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVSEMEG 60
           MG+H KGK ST SHH+RLLKETIGSSFPP SLLHSFKRSFNGFVAK+TE E KKVSEMEG
Sbjct: 38  MGSHSKGKVSTSSHHIRLLKETIGSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEG 97

Query: 61  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDTGYGP 120
           VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVES++IVGV D+GIWPESPSFD  GYG 
Sbjct: 98  VISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGS 157

Query: 121 PPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVAGGLV 180
           PPAKWKGSCEVSANFSCNNKIIGARSY S+G+  EGDIKGPRDSDGHGTHTAS VAGGLV
Sbjct: 158 PPAKWKGSCEVSANFSCNNKIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLV 217

Query: 181 QQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGSLGAP 240
           ++A+MLGLG GTARGGVPSARIA YKVCWSDGC+DADILAAFDDAIADGVDIISGSLG  
Sbjct: 218 RRASMLGLGLGTARGGVPSARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGS 277

Query: 241 GFVNYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDRVFETM 300
           G  +YFNDS+AIGSFHAMKKGIL+SLA GN GP F TI N+SPWSL+VAASTTDR FET 
Sbjct: 278 GARDYFNDSIAIGSFHAMKKGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETK 337

Query: 301 VELGDGRELKGASINTFDMKGKQIPLVYGGDIPKANISSSLSRECVENSVDLELAKGKIV 360
           VELGDGRE  G S+NTFD+KGKQIPLVY GDIPKA   SS+SR C EN+VDL+L KGKIV
Sbjct: 338 VELGDGREFSGVSVNTFDIKGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIV 397

Query: 361 VCDTVIDATEVVAVKGAVGIIMQDDSPMDG-HSYPIPASHVDSKSGAVILSYINSTNSIP 420
           VCD++     VVAVKGAVGIIMQDDS  D  +S+PIPASH+  K+GA++LSYINSTNSIP
Sbjct: 398 VCDSLTVPGGVVAVKGAVGIIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIP 457

Query: 421 TATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPISSPSGAVE 480
           TATIKKSTERKRKRAPSVASFSSRGPNP+T NILKPDLS PGVEILAAW P+S PSGA E
Sbjct: 458 TATIKKSTERKRKRAPSVASFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEE 517

Query: 481 DNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPKRNKEKEF 540
           DN+RVLYNIISGTSMACPHVTA AAYVKSFHPTWSP+ALKSAL+TTAFPMSPK N +KEF
Sbjct: 518 DNKRVLYNIISGTSMACPHVTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEF 577

Query: 541 AYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSNDSDTVFD 600
            YGAGH+NPLGAVHPGLIYDASE DYV+FLCGQGYTTELLQLVSEDNNTCSSN+SDTVFD
Sbjct: 578 GYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFD 637

Query: 601 LNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSVLSFNHLG 660
           LNYPSFAL+TN S+PINQVY+RTVTNVGSK ATYKAT+INPWKNLEIKVNPSVLSF +LG
Sbjct: 638 LNYPSFALSTNISKPINQVYKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLG 697

Query: 661 EEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           E+QSFEVTI+GKI  DIESASLVWDDGKHKVRSPITVF
Sbjct: 698 EKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVF 735

BLAST of MELO3C012343T1 vs. NCBI nr
Match: gi|659074279|ref|XP_008437519.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 5.6e-273
Identity = 484/705 (68.65%), Postives = 566/705 (80.28%), Query Frame = 1

Query: 1   MGNHPKGK----PSTPSHHMRLLKETIGSSFPPKSLLHSFKRSFNGFVAKMTEEEAKKVS 60
           MG+HPK +    P +  HHMRLL+E IGS+F P  LLHS+KRSFNGFVAK+TE EAKKVS
Sbjct: 37  MGSHPKDQVSSTPLSSHHHMRLLQEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVS 96

Query: 61  EMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRVPAVESDIIVGVFDTGIWPESPSFDDT 120
           EMEGVISVFPN + QLHTTRSWDFMGFSEQV+RVP+VESDIIVGVFDTGIWPESPSF D 
Sbjct: 97  EMEGVISVFPNEELQLHTTRSWDFMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDH 156

Query: 121 GYGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGQDLEGDIKGPRDSDGHGTHTASTVA 180
           GYGPPP KWKGSCEVS NFSCNNKIIGA+SY S G+    DIKGPRDS+GHGTH ASTVA
Sbjct: 157 GYGPPPPKWKGSCEVSLNFSCNNKIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVA 216

Query: 181 GGLVQQANMLGLGFGTARGGVPSARIAVYKVCWSDGCADADILAAFDDAIADGVDIISGS 240
           GGLV+QA+MLGLG GTARGGVPSARIA YKVCWSD C+ AD+LAAFDDAIADGVDIIS S
Sbjct: 217 GGLVRQASMLGLGTGTARGGVPSARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLS 276

Query: 241 LGAPGFV-NYFNDSMAIGSFHAMKKGILSSLAAGNTGPGFRTIANYSPWSLTVAASTTDR 300
           +G    + NYFND +AIG+FHAM+ GIL+S +AGN GP   T+ N+SPW+L+VAASTTDR
Sbjct: 277 VGPKRPIPNYFNDPIAIGTFHAMRNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDR 336

Query: 301 VFETMVELGDGRELKGASINTFDMKGKQIPLVYGGDIPK--ANISSSLSRECVENSVDLE 360
            F T V+LGDGR+  G +INTFD+ G Q PLV+ G+IP      + S+SR C+ NSVD E
Sbjct: 337 RFLTAVQLGDGRKFNGVTINTFDLNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRE 396

Query: 361 LAKGKIVVCDTVIDATEVVAVKGAVGIIMQDDSPMD-GHSYPIPASHVDSKSGAVILSYI 420
           L KGKI VCD+++  ++V +++ A+GIIMQD SP D   ++P+PASH+ ++   +I SY+
Sbjct: 397 LVKGKIAVCDSIVLPSDVGSLESALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYL 456

Query: 421 NSTNSIPTATIKKSTERKRKRAPSVASFSSRGPNPVTLNILKPDLSAPGVEILAAWPPIS 480
           N T  IPTATI KSTE K + AP VASFSSRGPNP T +ILKPD+  PGVEILAAW PI 
Sbjct: 457 NLTR-IPTATILKSTELKLEAAPLVASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIR 516

Query: 481 SPSGAVEDNQRVLYNIISGTSMACPHVTAVAAYVKSFHPTWSPAALKSALMTTAFPMSPK 540
           SPS A  DN+++L+NIISGTSMACPH TAVAAYVKSFHP+WSPAALKSAL+TTAFPM   
Sbjct: 517 SPSDAKGDNRKLLFNIISGTSMACPHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGD 576

Query: 541 RNKEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNTCSSN 600
              E EFAYG+GH+NPLGAV+PGLIY+ASE DY+RFLCG+GY T LL+++++DN+TCS  
Sbjct: 577 LYPEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPT 636

Query: 601 DSDTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSATYKATIINPWKNLEIKVNPSV 660
           +S  V+DLNY SFAL T  S P +QVY+R VTNVGS ++TYKATI  P   L I VNPS 
Sbjct: 637 NSGLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNSTYKATIFAP-SELNITVNPST 696

Query: 661 LSFNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 698
           LSF  L EE  FEVTI+GKI   I SASLVWDDG HKVRSPI VF
Sbjct: 697 LSFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVF 739

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CUCM1_CUCME3.8e-22956.96Cucumisin OS=Cucumis melo PE=1 SV=1[more]
SBT43_ARATH7.9e-19550.71Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1[more]
SBT44_ARATH1.2e-18748.30Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1[more]
SBT4C_ARATH2.9e-18147.91Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1[more]
SBT4D_ARATH5.0e-18147.17Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KRC4_CUCSA0.0e+0087.55Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1[more]
A0A0A0KWL9_CUCSA0.0e+0084.38Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1[more]
A0A0A0KJN5_CUCSA1.8e-27067.85Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1[more]
A0A0A0KLR4_CUCSA1.7e-25763.29Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1[more]
A0A0A0KLY4_CUCSA1.9e-24861.06Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.14.5e-19650.71 subtilase family protein[more]
AT5G59100.16.9e-18948.30 Subtilisin-like serine endopeptidase family protein[more]
AT5G59090.11.7e-18247.91 subtilase 4.12[more]
AT5G59120.12.8e-18247.17 subtilase 4.13[more]
AT3G46850.11.2e-18048.20 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|659091821|ref|XP_008446751.1|0.0e+00100.00PREDICTED: cucumisin-like [Cucumis melo][more]
gi|659091821|ref|XP_008446751.1|0.0e+0085.22PREDICTED: cucumisin-like [Cucumis melo][more]
gi|778708693|ref|XP_004135365.2|0.0e+0087.20PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus][more]
gi|778708693|ref|XP_004135365.2|0.0e+0084.20PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus][more]
gi|659074279|ref|XP_008437519.1|5.6e-27368.65PREDICTED: cucumisin-like isoform X1 [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C012343MELO3C012343gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C012343T1MELO3C012343T1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C012343T1.cds1MELO3C012343T1.cds1CDS
MELO3C012343T1.cds2MELO3C012343T1.cds2CDS
MELO3C012343T1.cds3MELO3C012343T1.cds3CDS
MELO3C012343T1.cds4MELO3C012343T1.cds4CDS
MELO3C012343T1.cds5MELO3C012343T1.cds5CDS
MELO3C012343T1.cds6MELO3C012343T1.cds6CDS
MELO3C012343T1.cds7MELO3C012343T1.cds7CDS
MELO3C012343T1.cds8MELO3C012343T1.cds8CDS
MELO3C012343T1.cds9MELO3C012343T1.cds9CDS
MELO3C012343T1.cds10MELO3C012343T1.cds10CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 94..303
score: 4.8E-78coord: 433..552
score: 4.8
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 95..544
score: 4.0
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 437..556
score: 1.31E-74coord: 72..332
score: 1.31
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 13..73
score: 3.2
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 490..506
score: 3.5E-13coord: 163..176
score: 3.5E-13coord: 94..113
score: 3.5
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..697
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 491..501
scor
NoneNo IPR availablePANTHERPTHR10795:SF348SUBFAMILY NOT NAMEDcoord: 1..697
score: