MELO3C004303T1 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C004303T1
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionDisease resistance protein (TIR-NBS-LRR class), putative
Locationchr5 : 25254173 .. 25257103 (-)
Sequence length2040
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGGGATATATGGCATTGGAGGCCTGGGTAAGACAACTTTGGCTAAAGCTTTGTACAATAAAATATCTAGCCAATTTGAAGGGTGCTGCTTTCTATCAAATGTTCGACAAGCTTCAAAGCAATTCAATGGCCTTGTTCAACTACAGGAAAACCTACTCTATGAAATCTTAAAGGATGATTTGAAGTTTGTCAATCTTGACAGAGGAATTACCATCATAAGGAGTAGACTGCATTCAAAGAAAGTTCTCATAGTTCTTGATGATGTGGATAAGCTCGAGCAATTAGAAGCATTGGTTGGTGGACGTGATTGGTTTGGTCAAGGTAGTAAAATCATTGTGACAACGAGGAATAGACATTTACTTTCTAGCCATGGATTTGATGAAATGCACAATATTCGAGGATTGTATCAAGACAAAGCTATTAAGCTTTTTAGTTGGCATGCTTTTAAGGAAAGTCATCCATCAAGTAATTATTTAGGCCTTGTCGAACGTGCTACAAGTTATTGTAAAGGTCATCCTTTAGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTACTAGAGATCAAACAGAATGGATTAGTATATTGGATGAATTTGAAAACTCTTTGAGCAATAATATTAAAGATATTCTTCAATTAAGTTTTGATGGGCTCGAAGACAGAGTAAAGGATATTTTTCTTGATATTTCTTGTTTACTCGTGGGAGAGGAAGTTAACTACGTTAAGAATATATTGAGTGCATGCCATCTCAATGTAGATTTCGGAATTATAATACTCATGGATCTTTCGCTTATTACGGTTGAAAATGGTACGGTGCAAATGCATGATCTAATACAGCAGATGGGCCATAAAATAGTTTATGGTGAATCTCCAGAGCCTGGAAAGAGGAGTAGGTTGTGGTTGGCGCAAGACATTTGGGAGGTGTTTGTTAATAATTCAGTGAGTAACTCTTACGCAAAGTATCGAAAATTTGCTTATTTCCAGGACTTTACACGACAACAAATGTTTGTCAAAGTAAGTTGTCAGTAATGTTAAATTACTAAATCTATAACTTTGTAGGGAACAGATACAATTAAAGCCATAAAGTTGGACTTGGCTAATCCTACAAGGTTACACGTGGATCCACAAGCATTTAGAAGCATGAAAAATTTGAGAGTACTTATTGTTCAAAATGCACGATTCTCTACAAAGATTAAACACTTATCAGATAGCTTAAAGTGGATTAAGTGGCATGGATTTGCTCATCGATCTTTGCCGTCATGCTTCATTACAAAAAGTCTTGTTGGGCTAGATATGCAACATAGCTTCATCAAAAAATTCGGGAAAAGACTTGAGGTAAATTATTCTTCTACGGGATGTTTACTTGATTGGAGGTTTCTTCATTGTTTTTATTAGAAATTAAATGGGTAGCTTTCATTTTAGAAAAGTTCAAAATATGTAGTTTTGTAATAAAAAAAATGATAATATGATAATTTTTAAAAATTAGTAAACGGGTAGCATTGTATTTTAAACAAACAATGTTATTTTGTTGATACACTGATCAAATGAAATCAATTTCATATCAAAAGGAAACAAAAAACAAAAACAATATTCACCATACTCGAACAAGCATTTGCCATAACGTAATTAAAGTCATGCTATATGATTACTTTATATTTGGTATATCAATCAACTTTGTATTTGGTAAATCAATCAACTTTACAAAGACAAGTCAAATATACTTTAAGATACATAAATGAGAGTAAAATTGAAATCATAGAAAATACGAAAATCAGATTCTTTTTTCAACTCATTACAATATTCCTTAACTAAAATAGTAGTAAGAAGTATGTTGCTATTTTATGCAATAGTTACGGATTAGTACTGTGGATTAATGTATATAGATTACTAATCTTGATATGGATTGAGTTTATCACTGATCTACTTATATATATCATTGATTTACTTTTGTATATGGGTATTATTAGTTTACTTGAATATTGATTATTGGTTATTATTGATTTACATATATTCTATTTTTCTATATTTACAAGCATTCTTTTAGTCGCTATTTCTGTAATATTTTATCTAAATTATGCTTCGTATGTCAGTTACGACCGTTTTTCCTTTTGGTTTCTTTTCCATGTGTTTTAAGATTGTGAAAGGTTGAAGCATGTTAATCTTAGCTACTCTTCTTTATTAGAGCAAATTCCTCATTTCCCTGCGGCATCAAACCTTGAAGAATTGCATCTAAGCGAATGCACGAATTTAAGAAAAATTGATAAGTCTGTTTTTTCTCTTGATAAGCTTACTATCCTAAACCTTGATGGCTGTTCTAACCTTAAAAAGCTTCCGACAAGCTACTTCATGGTAAGGTCTCTTAAACATTTGAAGCTCTCTTACTGTAAAAAACTTGAGAGAATTCCAGATTTATCCTTAGCATCAAACCTTGAGAGTTTATATCTTGAAGATTGCACAAATTTAAGAATGATTCATGAGTCTATTGGATCTTTGGATAAGCTTGTTACCTTAGTCCTTAGACGTTGCTTTAATCTTGCAAAACTTCCTAGCCATCTTCACTTAAAGTCCCTACAATATTTAGGACTTTCTGGGTGTCGGAAGCTTGAAAACTTCCCAACAATTGCTGAGAACTTGAAATCTATAAAATTGTTGGATTTGGATTTTACTGCCATAAAGGAGGTACCTCCATCAACTGGATATCTTACTCAGCTCTCTCGATTAAATATCAACGGTTGCACAAACCCCATCTCCCATCCCAATACAATTTGTTTGTCAAGATTGTATTTGTTAAGGAGTCTTGAAAATATTTTTCTTAGAGGGTGTTCTAGATTTGAAATTGTTCCCCATGAATGGGGCCAAATCATCCAACCAGCATGCTCTTTCTCAAAGATGATGGAAACAACTTCATGGAGCTCAGAATTTCCCCATTGA

mRNA sequence

ATGGTGGGGATATATGGCATTGGAGGCCTGGGTAAGACAACTTTGGCTAAAGCTTTGTACAATAAAATATCTAGCCAATTTGAAGGGTGCTGCTTTCTATCAAATGTTCGACAAGCTTCAAAGCAATTCAATGGCCTTGTTCAACTACAGGAAAACCTACTCTATGAAATCTTAAAGGATGATTTGAAGTTTGTCAATCTTGACAGAGGAATTACCATCATAAGGAGTAGACTGCATTCAAAGAAAGTTCTCATAGTTCTTGATGATGTGGATAAGCTCGAGCAATTAGAAGCATTGGTTGGTGGACGTGATTGGTTTGGTCAAGGTAGTAAAATCATTGTGACAACGAGGAATAGACATTTACTTTCTAGCCATGGATTTGATGAAATGCACAATATTCGAGGATTGTATCAAGACAAAGCTATTAAGCTTTTTAGTTGGCATGCTTTTAAGGAAAGTCATCCATCAAGTAATTATTTAGGCCTTGTCGAACGTGCTACAAGTTATTGTAAAGGTCATCCTTTAGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTACTAGAGATCAAACAGAATGGATTAGTATATTGGATGAATTTGAAAACTCTTTGAGCAATAATATTAAAGATATTCTTCAATTAAGTTTTGATGGGCTCGAAGACAGAGTAAAGGATATTTTTCTTGATATTTCTTGTTTACTCGTGGGAGAGGAAGTTAACTACGTTAAGAATATATTGAGTGCATGCCATCTCAATGTAGATTTCGGAATTATAATACTCATGGATCTTTCGCTTATTACGGTTGAAAATGGTACGGTGCAAATGCATGATCTAATACAGCAGATGGGCCATAAAATAGTTTATGGTGAATCTCCAGAGCCTGGAAAGAGGAGTAGGTTGTGGTTGGCGCAAGACATTTGGGAGGTGTTTGTTAATAATTCAGGAACAGATACAATTAAAGCCATAAAGTTGGACTTGGCTAATCCTACAAGGTTACACGTGGATCCACAAGCATTTAGAAGCATGAAAAATTTGAGAGTACTTATTGTTCAAAATGCACGATTCTCTACAAAGATTAAACACTTATCAGATAGCTTAAAGTGGATTAAGTGGCATGGATTTGCTCATCGATCTTTGCCGTCATGCTTCATTACAAAAAGTCTTGTTGGGCTAGATATGCAACATAGCTTCATCAAAAAATTCGGGAAAAGACTTGAGGTAAATTATTCTTCTACGGGATGTTTACTTGATTGGAGGTTGAAGCATGTTAATCTTAGCTACTCTTCTTTATTAGAGCAAATTCCTCATTTCCCTGCGGCATCAAACCTTGAAGAATTGCATCTAAGCGAATGCACGAATTTAAGAAAAATTGATAAGTCTGTTTTTTCTCTTGATAAGCTTACTATCCTAAACCTTGATGGCTGTTCTAACCTTAAAAAGCTTCCGACAAGCTACTTCATGGTAAGGTCTCTTAAACATTTGAAGCTCTCTTACTGTAAAAAACTTGAGAGAATTCCAGATTTATCCTTAGCATCAAACCTTGAGAGTTTATATCTTGAAGATTGCACAAATTTAAGAATGATTCATGAGTCTATTGGATCTTTGGATAAGCTTGTTACCTTAGTCCTTAGACGTTGCTTTAATCTTGCAAAACTTCCTAGCCATCTTCACTTAAAGTCCCTACAATATTTAGGACTTTCTGGGTGTCGGAAGCTTGAAAACTTCCCAACAATTGCTGAGAACTTGAAATCTATAAAATTGTTGGATTTGGATTTTACTGCCATAAAGGAGGTACCTCCATCAACTGGATATCTTACTCAGCTCTCTCGATTAAATATCAACGGTTGCACAAACCCCATCTCCCATCCCAATACAATTTGTTTGTCAAGATTGTATTTGTTAAGGAGTCTTGAAAATATTTTTCTTAGAGGGTGTTCTAGATTTGAAATTGTTCCCCATGAATGGGGCCAAATCATCCAACCAGCATGCTCTTTCTCAAAGATGATGGAAACAACTTCATGGAGCTCAGAATTTCCCCATTGA

Coding sequence (CDS)

ATGGTGGGGATATATGGCATTGGAGGCCTGGGTAAGACAACTTTGGCTAAAGCTTTGTACAATAAAATATCTAGCCAATTTGAAGGGTGCTGCTTTCTATCAAATGTTCGACAAGCTTCAAAGCAATTCAATGGCCTTGTTCAACTACAGGAAAACCTACTCTATGAAATCTTAAAGGATGATTTGAAGTTTGTCAATCTTGACAGAGGAATTACCATCATAAGGAGTAGACTGCATTCAAAGAAAGTTCTCATAGTTCTTGATGATGTGGATAAGCTCGAGCAATTAGAAGCATTGGTTGGTGGACGTGATTGGTTTGGTCAAGGTAGTAAAATCATTGTGACAACGAGGAATAGACATTTACTTTCTAGCCATGGATTTGATGAAATGCACAATATTCGAGGATTGTATCAAGACAAAGCTATTAAGCTTTTTAGTTGGCATGCTTTTAAGGAAAGTCATCCATCAAGTAATTATTTAGGCCTTGTCGAACGTGCTACAAGTTATTGTAAAGGTCATCCTTTAGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTACTAGAGATCAAACAGAATGGATTAGTATATTGGATGAATTTGAAAACTCTTTGAGCAATAATATTAAAGATATTCTTCAATTAAGTTTTGATGGGCTCGAAGACAGAGTAAAGGATATTTTTCTTGATATTTCTTGTTTACTCGTGGGAGAGGAAGTTAACTACGTTAAGAATATATTGAGTGCATGCCATCTCAATGTAGATTTCGGAATTATAATACTCATGGATCTTTCGCTTATTACGGTTGAAAATGGTACGGTGCAAATGCATGATCTAATACAGCAGATGGGCCATAAAATAGTTTATGGTGAATCTCCAGAGCCTGGAAAGAGGAGTAGGTTGTGGTTGGCGCAAGACATTTGGGAGGTGTTTGTTAATAATTCAGGAACAGATACAATTAAAGCCATAAAGTTGGACTTGGCTAATCCTACAAGGTTACACGTGGATCCACAAGCATTTAGAAGCATGAAAAATTTGAGAGTACTTATTGTTCAAAATGCACGATTCTCTACAAAGATTAAACACTTATCAGATAGCTTAAAGTGGATTAAGTGGCATGGATTTGCTCATCGATCTTTGCCGTCATGCTTCATTACAAAAAGTCTTGTTGGGCTAGATATGCAACATAGCTTCATCAAAAAATTCGGGAAAAGACTTGAGGTAAATTATTCTTCTACGGGATGTTTACTTGATTGGAGGTTGAAGCATGTTAATCTTAGCTACTCTTCTTTATTAGAGCAAATTCCTCATTTCCCTGCGGCATCAAACCTTGAAGAATTGCATCTAAGCGAATGCACGAATTTAAGAAAAATTGATAAGTCTGTTTTTTCTCTTGATAAGCTTACTATCCTAAACCTTGATGGCTGTTCTAACCTTAAAAAGCTTCCGACAAGCTACTTCATGGTAAGGTCTCTTAAACATTTGAAGCTCTCTTACTGTAAAAAACTTGAGAGAATTCCAGATTTATCCTTAGCATCAAACCTTGAGAGTTTATATCTTGAAGATTGCACAAATTTAAGAATGATTCATGAGTCTATTGGATCTTTGGATAAGCTTGTTACCTTAGTCCTTAGACGTTGCTTTAATCTTGCAAAACTTCCTAGCCATCTTCACTTAAAGTCCCTACAATATTTAGGACTTTCTGGGTGTCGGAAGCTTGAAAACTTCCCAACAATTGCTGAGAACTTGAAATCTATAAAATTGTTGGATTTGGATTTTACTGCCATAAAGGAGGTACCTCCATCAACTGGATATCTTACTCAGCTCTCTCGATTAAATATCAACGGTTGCACAAACCCCATCTCCCATCCCAATACAATTTGTTTGTCAAGATTGTATTTGTTAAGGAGTCTTGAAAATATTTTTCTTAGAGGGTGTTCTAGATTTGAAATTGTTCCCCATGAATGGGGCCAAATCATCCAACCAGCATGCTCTTTCTCAAAGATGATGGAAACAACTTCATGGAGCTCAGAATTTCCCCATTGA

Protein sequence

MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKDDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNYVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQIIQPACSFSKMMETTSWSSEFPH*
BLAST of MELO3C004303T1 vs. Swiss-Prot
Match: TMVRN_NICGU (TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-98
Identity = 231/624 (37.02%), Postives = 356/624 (57.05%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKI------SSQFEGCCFLSNVRQASKQFNGLVQLQENLL 60
           ++GI+G+GG+GKTT+A+A+++ +      S QF+G CFL ++++  +   G+  LQ  LL
Sbjct: 211 IMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKENKR---GMHSLQNALL 270

Query: 61  YEILKDDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQ-LEALVGGRDWFGQGSKII 120
            E+L++   + N + G   + SRL SKKVLIVLDD+D  +  LE L G  DWFG GS+II
Sbjct: 271 SELLREKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRII 330

Query: 121 VTTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGH 180
           +TTR++HL+  +  D ++ +  L   ++I+LF  HAF +  P+ N+  L     +Y KG 
Sbjct: 331 ITTRDKHLIEKN--DIIYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGL 390

Query: 181 PLALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLL 240
           PLAL V GS L     TEW S ++  +N+  + I D L++S+DGLE + +++FLDI+C L
Sbjct: 391 PLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFL 450

Query: 241 VGEEVNYVKNILSACHLNVDFGIIILMDLSLITV-ENGTVQMHDLIQQMGHKIVYGESPE 300
            GEE +Y+  IL +CH+  ++G+ IL+D SL+ + E   VQMHDLIQ MG K +     +
Sbjct: 451 RGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMG-KYIVNFQKD 510

Query: 301 PGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNA 360
           PG+RSRLWLA+++ EV  NN+GT  ++AI +   + T L    QA ++MK LRV  +  +
Sbjct: 511 PGERSRLWLAKEVEEVMSNNTGTMAMEAIWVSSYSST-LRFSNQAVKNMKRLRVFNMGRS 570

Query: 361 RFSTKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTG 420
                I +L ++L+      +   S PS F  K LV L ++H+ ++         ++ T 
Sbjct: 571 STHYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRHNSLRHL-------WTETK 630

Query: 421 CLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLD 480
            L    L+ ++LS+S  L + P F    NLE ++L +C+NL ++  S+    K+  L L+
Sbjct: 631 HLPS--LRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSKVIGLYLN 690

Query: 481 GCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIG 540
            C +LK+ P     V SL++L L  C  LE++P++      E       + +R +  SI 
Sbjct: 691 DCKSLKRFPC--VNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRELPSSIF 750

Query: 541 SLDKLVT-LVLRRCFNLAKLPSHL-HLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLD 600
                VT L+L    NL  LPS +  LKSL  L +SGC KLE+ P    +L ++++ D  
Sbjct: 751 QYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDNLRVFDAS 810

Query: 601 FTAIKEVPPSTGYLTQLSRLNING 615
            T I   P S   L +L  L   G
Sbjct: 811 DTLILRPPSSIIRLNKLIILMFRG 816


HSP 2 Score: 107.5 bits (267), Expect = 6.0e-22
Identity = 78/242 (32.23%), Postives = 123/242 (50.83%), Query Frame = 1

Query: 418 RLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLR-KIDKSVFSLDKLTILNLDGCSN 477
           RL+  N+  SS    I + P       L    CTN   +   S F L  L  L L   ++
Sbjct: 554 RLRVFNMGRSSTHYAIDYLP-----NNLRCFVCTNYPWESFPSTFELKMLVHLQLRH-NS 613

Query: 478 LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 537
           L+ L T    + SL+ + LS+ K+L R PD +   NLE + L  C+NL  +H S+G   K
Sbjct: 614 LRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCCSK 673

Query: 538 LVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKE 597
           ++ L L  C +L + P  ++++SL+YLGL  C  LE  P I   +K    + +  + I+E
Sbjct: 674 VIGLYLNDCKSLKRFPC-VNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIRE 733

Query: 598 VPPST-GYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWG 657
           +P S   Y T +++L +    N ++ P++IC      L+SL ++ + GCS+ E +P E G
Sbjct: 734 LPSSIFQYKTHVTKLLLWNMKNLVALPSSIC-----RLKSLVSLSVSGCSKLESLPEEIG 783

BLAST of MELO3C004303T1 vs. Swiss-Prot
Match: TAO1_ARATH (Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 5.9e-86
Identity = 221/697 (31.71%), Postives = 368/697 (52.80%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNV-----RQASKQFNGLVQLQENLLY 60
           M+GI+G  G+GKTT+++ LYNK+  QF+    + N+     R    +++  +QLQ+ LL 
Sbjct: 236 MIGIWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSAKLQLQKELLS 295

Query: 61  EILKD-DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIV 120
           +++   D+   +L     + + RL  KKVL+VLDDVD L QL+A+     WFG GS+IIV
Sbjct: 296 QMINQKDMVVPHLG----VAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIV 355

Query: 121 TTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHP 180
            T++  LL +HG   ++ +     D+A+++F  +AF E  P   +  +    T+     P
Sbjct: 356 VTQDLKLLKAHGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLP 415

Query: 181 LALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLV 240
           L L V+GS+L    + EW   +     SL ++I+ +L+ S++ L ++ KD+FL I+C   
Sbjct: 416 LGLRVMGSYLRRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQEKDLFLHITCFFR 475

Query: 241 GEEVNYVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGES-PEP 300
            E +  ++  L+   +++  G+ IL D SL+++  G ++MH+L+ Q+G  IV  +S  +P
Sbjct: 476 RERIETLEVFLAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKP 535

Query: 301 GKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTR--LHVDPQAFRSMKNLRVLIVQN 360
           GKR  L   +DI EV  +++GT T+  I L+L+      +++  +AF  M NL+ L   +
Sbjct: 536 GKRQFLVDTEDICEVLTDDTGTRTLIGIDLELSGVIEGVINISERAFERMCNLQFLRFHH 595

Query: 361 AR---------FSTKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGK 420
                          + H+S  L+ + W  +    LP  F  + LV ++M+ S ++K   
Sbjct: 596 PYGDRCHDILYLPQGLSHISRKLRLLHWERYPLTCLPPKFNPEFLVKINMRDSMLEKLWD 655

Query: 421 RLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSEC------------ 480
             E   +         LK ++LS+   L+++P F  A+NL+EL L  C            
Sbjct: 656 GNEPIRN---------LKWMDLSFCVNLKELPDFSTATNLQELRLINCLSLVELPSSIGN 715

Query: 481 -TNLRKID-----------KSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYC 540
            TNL ++D            S+ +L  L  L L+ CS+L KLP+S+  V SLK L LS C
Sbjct: 716 ATNLLELDLIDCSSLVKLPSSIGNLTNLKKLFLNRCSSLVKLPSSFGNVTSLKELNLSGC 775

Query: 541 KKLERIP-DLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSH-LH 600
             L  IP  +    NL+ +Y + C++L  +  SIG+   L  L L  C +L + PS  L+
Sbjct: 776 SSLLEIPSSIGNIVNLKKVYADGCSSLVQLPSSIGNNTNLKELHLLNCSSLMECPSSMLN 835

Query: 601 LKSLQYLGLSGCRKLENFPTIAE--NLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNING 652
           L  L+ L LSGC  L   P+I    NL+S+ L D   +++ E+P +    T L  L ++G
Sbjct: 836 LTRLEDLNLSGCLSLVKLPSIGNVINLQSLYLSDC--SSLMELPFTIENATNLDTLYLDG 895


HSP 2 Score: 98.6 bits (244), Expect = 2.8e-19
Identity = 73/222 (32.88%), Postives = 115/222 (51.80%), Query Frame = 1

Query: 409 SSTGCLLDWRLKHVNLSYSSLLEQIPH-FPAASNLEELHLSECTNLRKIDKSVFSLDKLT 468
           SS G +++  LK V     S L Q+P      +NL+ELHL  C++L +   S+ +L +L 
Sbjct: 770 SSIGNIVN--LKKVYADGCSSLVQLPSSIGNNTNLKELHLLNCSSLMECPSSMLNLTRLE 829

Query: 469 ILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMI 528
            LNL GC +L KLP+                        +    NL+SLYL DC++L  +
Sbjct: 830 DLNLSGCLSLVKLPS------------------------IGNVINLQSLYLSDCSSLMEL 889

Query: 529 HESIGSLDKLVTLVLRRCFNLAKLPSHL-HLKSLQYLGLSGCRKLENFPTIAENLKSIKL 588
             +I +   L TL L  C NL +LPS + ++ +LQ L L+GC  L+  P++ EN  +++ 
Sbjct: 890 PFTIENATNLDTLYLDGCSNLLELPSSIWNITNLQSLYLNGCSSLKELPSLVENAINLQS 949

Query: 589 LDL-DFTAIKEVPPSTGYLTQLSRLNINGCT-----NPISHP 623
           L L   +++ E+P S   ++ LS L+++ C+     N +SHP
Sbjct: 950 LSLMKCSSLVELPSSIWRISNLSYLDVSNCSSLLELNLVSHP 965

BLAST of MELO3C004303T1 vs. Swiss-Prot
Match: WRK19_ARATH (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.6e-83
Identity = 216/636 (33.96%), Postives = 333/636 (52.36%), Query Frame = 1

Query: 2    VGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKDD 61
            +GI+G  G+GKTT+A+ ++ KIS Q+E C  L ++ +   +  G   ++EN L E+L+ +
Sbjct: 840  IGIWGTVGIGKTTIAEEIFRKISVQYETCVVLKDLHK-EVEVKGHDAVRENFLSEVLEVE 899

Query: 62   LKFVNL-DRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 121
               + + D   + +RSRL  K++L++LDDV+    ++  +G  ++FG GS+II+T+RNR 
Sbjct: 900  PHVIRISDIKTSFLRSRLQRKRILVILDDVNDYRDVDTFLGTLNYFGPGSRIIMTSRNRR 959

Query: 122  LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 181
            +      D ++ ++ L   K++ L      +       Y  L      +  G+P  L  L
Sbjct: 960  VFVLCKIDHVYEVKPLDIPKSLLLLDRGTCQIVLSPEVYKTLSLELVKFSNGNPQVLQFL 1019

Query: 182  GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 241
             S        EW  +  E + +    I  I + S  GL+D  + IFLDI+C     + + 
Sbjct: 1020 SSI-----DREWNKLSQEVKTTSPIYIPGIFEKSCCGLDDNERGIFLDIACFFNRIDKDN 1079

Query: 242  VKNILSACHLNVDFGIIILMDLSLITV-ENGTVQMHDLIQQMGHKIVYGESPE-PGKRSR 301
            V  +L  C  +   G   L+D SL+T+ ++  V M   IQ  G +IV  ES + PG RSR
Sbjct: 1080 VAMLLDGCGFSAHVGFRGLVDKSLLTISQHNLVDMLSFIQATGREIVRQESADRPGDRSR 1139

Query: 302  LWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTK- 361
            LW A  I  VF+N++GT  I+ I LD+ N  +   +P  F  M NLR+L +  ++   K 
Sbjct: 1140 LWNADYIRHVFINDTGTSAIEGIFLDMLN-LKFDANPNVFEKMCNLRLLKLYCSKAEEKH 1199

Query: 362  -------IKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSS 421
                   +++L   L+ + W  +   SLP  F  ++LV L++  S  KK  K  +  + +
Sbjct: 1200 GVSFPQGLEYLPSKLRLLHWEYYPLSSLPKSFNPENLVELNLPSSCAKKLWKGKKARFCT 1259

Query: 422  TGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILN 481
            T   L+ +LK + LSYS  L +IP   +A+NLE + L  C +L  + +S+  L KL  LN
Sbjct: 1260 TNSSLE-KLKKMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSISYLKKLVFLN 1319

Query: 482  LDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHES 541
            L GCS L+ +P S   + SL+ L LS C KL   P++S   N++ LY+   T ++ I  S
Sbjct: 1320 LKGCSKLENIP-SMVDLESLEVLNLSGCSKLGNFPEIS--PNVKELYMGG-TMIQEIPSS 1379

Query: 542  IGSLDKLVTLVLRRCFNLAKLPSHLH-LKSLQYLGLSGCRKLENFPTIAENLKSIKLLDL 601
            I +L  L  L L    +L  LP+ ++ LK L+ L LSGC  LE FP  +  +K ++ LDL
Sbjct: 1380 IKNLVLLEKLDLENSRHLKNLPTSIYKLKHLETLNLSGCISLERFPDSSRRMKCLRFLDL 1439

Query: 602  DFTAIKEVPPSTGYLTQL-------SRLNINGCTNP 619
              T IKE+P S  YLT L       SR N    TNP
Sbjct: 1440 SRTDIKELPSSISYLTALDELLFVDSRRNSPVVTNP 1463


HSP 2 Score: 109.8 bits (273), Expect = 1.2e-22
Identity = 87/239 (36.40%), Postives = 122/239 (51.05%), Query Frame = 1

Query: 420  KHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLK- 479
            KH  +S+   LE +P     S L  LH  E   L  + KS F+ + L  LNL      K 
Sbjct: 1191 KH-GVSFPQGLEYLP-----SKLRLLHW-EYYPLSSLPKS-FNPENLVELNLPSSCAKKL 1250

Query: 480  ------KLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIG 539
                  +  T+   +  LK ++LSY  +L +IP LS A+NLE + LE C +L  + +SI 
Sbjct: 1251 WKGKKARFCTTNSSLEKLKKMRLSYSDQLTKIPRLSSATNLEHIDLEGCNSLLSLSQSIS 1310

Query: 540  SLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFT 599
             L KLV L L+ C  L  +PS + L+SL+ L LSGC KL NFP I+ N+K    L +  T
Sbjct: 1311 YLKKLVFLNLKGCSKLENIPSMVDLESLEVLNLSGCSKLGNFPEISPNVKE---LYMGGT 1370

Query: 600  AIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVP 652
             I+E+P S   L  L +L++    +  + P +I     Y L+ LE + L GC   E  P
Sbjct: 1371 MIQEIPSSIKNLVLLEKLDLENSRHLKNLPTSI-----YKLKHLETLNLSGCISLERFP 1413

BLAST of MELO3C004303T1 vs. Swiss-Prot
Match: RPP1_ARATH (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana GN=RPP1 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.4e-79
Identity = 212/682 (31.09%), Postives = 356/682 (52.20%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNV-----RQASKQFNGLVQLQENLLY 60
           M+GI+G  G+GKTT+A+ L+N++S +F+    + N+     R    +++  +QLQ  +L 
Sbjct: 295 MIGIWGPPGIGKTTIARFLFNQVSDRFQLSAIMVNIKGCYPRPCFDEYSAQLQLQNQMLS 354

Query: 61  EILKD-DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIV 120
           +++   D+   +L     + + RL  KKV +VLD+VD+L QL+AL     WFG GS+II+
Sbjct: 355 QMINHKDIMISHLG----VAQERLRDKKVFLVLDEVDQLGQLDALAKETRWFGPGSRIII 414

Query: 121 TTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHP 180
           TT +  +L +HG + ++ +     D+A ++F  +AF +  P   +  +    T      P
Sbjct: 415 TTEDLGVLKAHGINHVYKVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVTCLAGELP 474

Query: 181 LALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLV 240
           L L VLGS L  + + EW   L   + SL   I  I+Q S+D L D  K +FL I+CL  
Sbjct: 475 LGLKVLGSALRGKSKREWERTLPRLKTSLDGKIGSIIQFSYDVLCDEDKYLFLYIACLFN 534

Query: 241 GEEVNYVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPG 300
           GE    VK +L    L+V  G+ +L   SLI+ +   + MH L++Q G +    +    G
Sbjct: 535 GESTTKVKELLGK-FLDVKQGLHLLAQKSLISFDGERIHMHTLLEQFGRETSRKQFVHHG 594

Query: 301 --KRSRLWLAQDIWEVFVNNSGTDTIK--AIKLDLAN-PTRLHVDPQAFRSMKNLRVLIV 360
             KR  L  A+ I EV +++  TD+ +   I L+L+N    L++  +    + +   + +
Sbjct: 595 FTKRQLLVGARGICEV-LDDDTTDSRRFIGIHLELSNTEEELNISEKVLERVHDFHFVRI 654

Query: 361 ----QNARFSTKIKHL---SDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGK 420
               Q  R    ++ L   S  ++ + W+G+    LPS F  + LV LDM+ S ++K   
Sbjct: 655 DASFQPERLQLALQDLIYHSPKIRSLNWYGYESLCLPSTFNPEFLVELDMRSSNLRKL-- 714

Query: 421 RLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFS 480
                +  T  L +  LK ++LSYSS L+++P+   A+NLEEL L  C++L ++  S+  
Sbjct: 715 -----WEGTKQLRN--LKWMDLSYSSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEK 774

Query: 481 LDKLTILNLDGCSNLKKLPT-----------------------SYFMVRSLKHLKLSYCK 540
           L  L IL+L+ CS+L+KLP                        S     +LK L +S C 
Sbjct: 775 LTSLQILDLENCSSLEKLPAIENATKLRELKLQNCSSLIELPLSIGTATNLKQLNISGCS 834

Query: 541 KLERIP-DLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLK 600
            L ++P  +   ++LE   L +C++L  +  SIG+L  L  L++R C  L  LP +++LK
Sbjct: 835 SLVKLPSSIGDITDLEVFDLSNCSSLVTLPSSIGNLQNLCKLIMRGCSKLEALPININLK 894

Query: 601 SLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNP 640
           SL  L L+ C +L++FP I+ ++  ++L     TAIKEVP S    + L+   I+   + 
Sbjct: 895 SLDTLNLTDCSQLKSFPEISTHISELRLKG---TAIKEVPLSIMSWSPLADFQISYFESL 954


HSP 2 Score: 76.6 bits (187), Expect = 1.1e-12
Identity = 62/233 (26.61%), Postives = 111/233 (47.64%), Query Frame = 1

Query: 418  RLKHVNLSYSSLLEQIP-HFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSN 477
            +L+ + L   S L ++P     A+NL++L++S C++L K+  S+  +  L + +L  CS+
Sbjct: 785  KLRELKLQNCSSLIELPLSIGTATNLKQLNISGCSSLVKLPSSIGDITDLEVFDLSNCSS 844

Query: 478  LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 537
            L  LP+S   +++L  L +  C KLE +P      +L++L L DC+ L+    S   +  
Sbjct: 845  LVTLPSSIGNLQNLCKLIMRGCSKLEALPININLKSLDTLNLTDCSQLK----SFPEIST 904

Query: 538  LVTLVLRRCFNLAKLP-SHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIK 597
             ++ +  +   + ++P S +    L    +S    L  FP   + +  + L       I+
Sbjct: 905  HISELRLKGTAIKEVPLSIMSWSPLADFQISYFESLMEFPHAFDIITKLHL----SKDIQ 964

Query: 598  EVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFE 649
            EVPP    +++L  L++N C N +S P         L  SL+ I+   C   E
Sbjct: 965  EVPPWVKRMSRLRDLSLNNCNNLVSLPQ--------LSDSLDYIYADNCKSLE 1001


HSP 3 Score: 50.8 bits (120), Expect = 6.7e-05
Identity = 38/136 (27.94%), Postives = 62/136 (45.59%), Query Frame = 1

Query: 522 TNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENL 581
           +NLR + E    L  L  + L     L +LP+     +L+ L L  C  L   P+  E L
Sbjct: 701 SNLRKLWEGTKQLRNLKWMDLSYSSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEKL 760

Query: 582 KSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFL 641
            S+++LDL+  +  E  P+    T+L  L +  C++ I  P +I  +      +L+ + +
Sbjct: 761 TSLQILDLENCSSLEKLPAIENATKLRELKLQNCSSLIELPLSIGTA-----TNLKQLNI 820

Query: 642 RGCSRFEIVPHEWGQI 658
            GCS    +P   G I
Sbjct: 821 SGCSSLVKLPSSIGDI 831

BLAST of MELO3C004303T1 vs. Swiss-Prot
Match: Y4117_ARATH (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.5e-78
Identity = 207/634 (32.65%), Postives = 335/634 (52.84%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFN----GL-VQLQENLLY 60
           +VGI+G  G+GKTT+A+ALYN+    F    F+ NVR++  +      GL + LQ+  L 
Sbjct: 207 IVGIWGPAGVGKTTIARALYNQYHENFNLSIFMENVRESYGEAGLDDYGLKLHLQQRFLS 266

Query: 61  EIL-KDDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIV 120
           ++L + DL+     R +  I  RL S+KVLI+LDDVD +EQL+AL     WFG  S+I+V
Sbjct: 267 KLLDQKDLRV----RHLGAIEERLKSQKVLIILDDVDNIEQLKALAKENQWFGNKSRIVV 326

Query: 121 TTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHP 180
           TT+N+ LL SH  + M+ +    + +A+ +F  HAFK+S PS +   L    T+     P
Sbjct: 327 TTQNKQLLVSHDINHMYQVAYPSKQEALTIFCQHAFKQSSPSDDLKHLAIEFTTLAGHLP 386

Query: 181 LALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLV 240
           LAL VLGSF+  + + EW   L   ++ L   ++ +L++ +DGL D  KD+FL I+C+  
Sbjct: 387 LALRVLGSFMRGKGKEEWEFSLPTLKSRLDGEVEKVLKVGYDGLHDHEKDLFLHIACIFS 446

Query: 241 GEEVNYVKNILSACH-LNVDFGIIILMDLSLI-TVENGTVQMHDLIQQMGHKIVYGES-P 300
           G+  NY+K ++ A +   V FG+ +L D SLI   ENG ++MH L++Q+G ++V  +S  
Sbjct: 447 GQHENYLKQMIIANNDTYVSFGLQVLADKSLIQKFENGRIEMHSLLRQLGKEVVRKQSIY 506

Query: 301 EPGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLAN-PTRLHVDPQAFRSMKNLRVL-IV 360
           EPGKR  L  A++   V  NN+GT T+  I LD+      L++  + F  M+NL  L   
Sbjct: 507 EPGKRQFLMNAKETCGVLSNNTGTGTVLGISLDMCEIKEELYISEKTFEEMRNLVYLKFY 566

Query: 361 QNARFSTKIK----------HLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKK 420
            ++    K+K               L+ + W  +     PS F  + LV L+M HS +KK
Sbjct: 567 MSSPIDDKMKVKLQLPEEGLSYLPQLRLLHWDAYPLEFFPSSFRPECLVELNMSHSKLKK 626

Query: 421 FGKRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKS 480
                   +S    L +  L+ +NL+ S  LE +P+   A+ L  L L  C +L ++  S
Sbjct: 627 L-------WSGVQPLRN--LRTMNLNSSRNLEILPNLMEATKLNRLDLGWCESLVELPSS 686

Query: 481 VFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLE 540
           + +L  L +L +  C  L+ +PT+   + SL+ L   YC +L+  P++S    L +L   
Sbjct: 687 IKNLQHLILLEMSCCKKLEIIPTN-INLPSLEVLHFRYCTRLQTFPEISTNIRLLNLIGT 746

Query: 541 DCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLK-SLQYLGLSGCRKLENFPTIA 600
             T +    +    +D++       C   AK+   +H+   L+ L L   ++LE  P   
Sbjct: 747 AITEVPPSVKYWSKIDEI-------CMERAKVKRLVHVPYVLEKLCLRENKELETIPRYL 806

Query: 601 ENLKSIKLLDLDFTA-IKEVPPSTGYLTQLSRLN 612
           + L  ++++D+ +   I  +P   G ++ L+ +N
Sbjct: 807 KYLPRLQMIDISYCINIISLPKLPGSVSALTAVN 819


HSP 2 Score: 79.7 bits (195), Expect = 1.3e-13
Identity = 64/199 (32.16%), Postives = 99/199 (49.75%), Query Frame = 1

Query: 459 SVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYL 518
           S F  + L  LN+   S LKKL +    +R+L+ + L+  + LE +P+L  A+ L  L L
Sbjct: 603 SSFRPECLVELNMSH-SKLKKLWSGVQPLRNLRTMNLNSSRNLEILPNLMEATKLNRLDL 662

Query: 519 EDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIA 578
             C +L  +  SI +L  L+ L +  C  L  +P++++L SL+ L    C +L+ FP I+
Sbjct: 663 GWCESLVELPSSIKNLQHLILLEMSCCKKLEIIPTNINLPSLEVLHFRYCTRLQTFPEIS 722

Query: 579 ENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLR---- 638
            N   I+LL+L  TAI EVPPS  Y +++               + IC+ R  + R    
Sbjct: 723 TN---IRLLNLIGTAITEVPPSVKYWSKI---------------DEICMERAKVKRLVHV 782

Query: 639 --SLENIFLRGCSRFEIVP 652
              LE + LR     E +P
Sbjct: 783 PYVLEKLCLRENKELETIP 782

BLAST of MELO3C004303T1 vs. TrEMBL
Match: A0A0A0LLL0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 1.3e-289
Identity = 514/681 (75.48%), Postives = 576/681 (84.58%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVGIYGIGGLGKTTLAKALYNKI+SQFEGCCFLSNVRQAS QFNGLVQLQ+NLLYEIL+D
Sbjct: 230 MVGIYGIGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILED 289

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLKFVNLD+GITIIR+RL SKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRN H
Sbjct: 290 DLKFVNLDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSH 349

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSHGFDEMHNI+GL QD+AI+LFSWHAFKESHPSSNYL L ERATSYCKGHPLALVVL
Sbjct: 350 LLSSHGFDEMHNIQGLNQDRAIELFSWHAFKESHPSSNYLDLAERATSYCKGHPLALVVL 409

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEV-N 240
           GSFLC R QTEW SILD+FENSL+N+IKDILQLSFDGLE  VKDIFLDISCL VGE+  N
Sbjct: 410 GSFLCNRGQTEWRSILDKFENSLNNDIKDILQLSFDGLEGGVKDIFLDISCLFVGEKYNN 469

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRL 300
             K +LSACHLNVDFGI+ILMDLSL+T+E   VQMH LIQQMGH IV+ ES E GKRSRL
Sbjct: 470 CAKKMLSACHLNVDFGIMILMDLSLVTIEKDRVQMHGLIQQMGHSIVHNESFESGKRSRL 529

Query: 301 WLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQA-FRSMKNLRVLIVQNARFSTKI 360
           W  +DIW VFVNNSGTD IKAIKLDL NP  ++VDP+A FRSMKNLR+LI++NA+  TKI
Sbjct: 530 WSERDIWNVFVNNSGTDAIKAIKLDLPNPINVNVDPKAFFRSMKNLRLLIIRNAQVCTKI 589

Query: 361 KHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWR 420
           K+L +SLKWI+W GFAHR+ PSCFITK+LVGLD++ SFIK+FGKRLE       C    R
Sbjct: 590 KYLPNSLKWIEWQGFAHRTFPSCFITKNLVGLDLRRSFIKRFGKRLE------DC---ER 649

Query: 421 LKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLK 480
           LKHV+LSYS+LLE+IP   AASNLEEL+L  CTNL  IDKSVFSL+KLT+LN  GCSNLK
Sbjct: 650 LKHVDLSYSTLLEKIPDLSAASNLEELYLINCTNLGMIDKSVFSLNKLTVLNFKGCSNLK 709

Query: 481 KLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLV 540
           KLP  YFM  SLK L LSYC++LE+IPDLS ASNL+SL L  CTNLR+IHES+GSL++LV
Sbjct: 710 KLPKGYFMFSSLKILNLSYCQELEKIPDLSSASNLQSLLLNGCTNLRVIHESVGSLNELV 769

Query: 541 TLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVP 600
            L L +C NL+KLPS+L LKSL YL L GC KLE+FPTIAEN+KS++ LDL  TAIKE+P
Sbjct: 770 LLDLGQCTNLSKLPSYLRLKSLVYLVLFGCGKLESFPTIAENMKSLRCLDLHSTAIKELP 829

Query: 601 PSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 660
            S GYLTQL +L++ GCTN IS PNTI     YLLR+L  + L GCSRFE+ PH+W   I
Sbjct: 830 SSLGYLTQLDKLHLTGCTNLISLPNTI-----YLLRNLNELHLGGCSRFEMFPHKWVPTI 889

Query: 661 QPACSFSKMMETTSWSSEFPH 680
           QP CS SKMME  SWS EFPH
Sbjct: 890 QPVCSPSKMMEAASWSLEFPH 896

BLAST of MELO3C004303T1 vs. TrEMBL
Match: A0A0A0LLL0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.6e-03
Identity = 54/230 (23.48%), Postives = 98/230 (42.61%), Query Frame = 1

Query: 432  QIPHFPA-ASNLEELHLSEC--TNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVR 491
            ++  FP  A N++ L   +   T ++++  S+  L +L  L+L GC+NL  LP + +++R
Sbjct: 792  KLESFPTIAENMKSLRCLDLHSTAIKELPSSLGYLTQLDKLHLTGCTNLISLPNTIYLLR 851

Query: 492  SLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNL 551
            +L  L L  C + E  P       ++ +    C+  +M+  +  SL+    +V       
Sbjct: 852  NLNELHLGGCSRFEMFPH-KWVPTIQPV----CSPSKMMEAASWSLEFPHLVVPNE---- 911

Query: 552  AKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLS 611
              + SH  L  L+   +S  + LE    +A  L  ++L +  F+++   P        LS
Sbjct: 912  -SICSHFTLLDLKSCNISSAKFLEILCDVAPFLSDLRLSENKFSSL---PSCLHKFMSLS 971

Query: 612  RLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 659
             L +  C      PN        L +++ N+   GC      P     II
Sbjct: 972  NLELRNCKFLQEIPN--------LPQNIRNLDASGCKSLARSPDNIVDII 1000


HSP 2 Score: 927.2 bits (2395), Expect = 1.2e-266
Identity = 485/679 (71.43%), Postives = 540/679 (79.53%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVG+YGIGG+GKTTLAKALYNKI+SQFEGCCFLSNVR+ASKQFNGL QLQE LLYEIL  
Sbjct: 230 MVGLYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQETLLYEILTV 289

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLK VNLDRGI IIR+RL  KKVLIVLDDVDKLEQLEALVGGRDWFGQGS+IIVTTRN+H
Sbjct: 290 DLKVVNLDRGINIIRNRLCLKKVLIVLDDVDKLEQLEALVGGRDWFGQGSRIIVTTRNKH 349

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSHGFDEM NI GL +DKAI+LFSWHAFK++HPSSNYL L +RATSYCKGHPLALVVL
Sbjct: 350 LLSSHGFDEMKNILGLDEDKAIELFSWHAFKKNHPSSNYLDLSKRATSYCKGHPLALVVL 409

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
           GSFLCTRDQ EW SILDEFENSL+ +IKDILQLSFDGLED+VKDIFLDISCLLVGE+  Y
Sbjct: 410 GSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKVKDIFLDISCLLVGEKGKY 469

Query: 241 VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
           VK++LSACH                               MGHKIV GES E GKRSRLW
Sbjct: 470 VKDMLSACH-------------------------------MGHKIVCGESLELGKRSRLW 529

Query: 301 LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
           L QD+WE      GTD +K IKLD  N TRL VDPQAFR MKNLR+LIVQNARFSTKI++
Sbjct: 530 LVQDVWE------GTDAVKGIKLDFPNSTRLDVDPQAFRKMKNLRLLIVQNARFSTKIEY 589

Query: 361 LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLK 420
           L DSLKWIKWHGF   + PS F  K+LVGLD+QHSFIK FGKRLE       C    RLK
Sbjct: 590 LPDSLKWIKWHGFRQPTFPSFFTMKNLVGLDLQHSFIKTFGKRLE------DC---ERLK 649

Query: 421 HVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKL 480
            V+LSYS+ LE+IP+F AASNLEEL+LS CTNL  IDKSVFSLDKLT+LNLDGCSNLKKL
Sbjct: 650 LVDLSYSTFLEKIPNFSAASNLEELYLSNCTNLGMIDKSVFSLDKLTVLNLDGCSNLKKL 709

Query: 481 PTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTL 540
           P  YFM+ SLK L LSYCKKLE+IPDLS ASNL SL++ +CTNLR+IHES+GSLDKL  L
Sbjct: 710 PRGYFMLSSLKKLNLSYCKKLEKIPDLSSASNLTSLHIYECTNLRVIHESVGSLDKLEGL 769

Query: 541 VLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPS 600
            L++C NL KLPS+L LKSL  L LSGC KLE+FPTIAEN+KS++ L+LDFTAIKE+P S
Sbjct: 770 YLKQCTNLVKLPSYLSLKSLLCLSLSGCCKLESFPTIAENMKSLRTLNLDFTAIKELPSS 829

Query: 601 TGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQIIQP 660
             YLT+L  L +NGCTN IS PNTI     YLLRSL+N+ L GCS F + P +W   IQP
Sbjct: 830 IRYLTKLWTLKLNGCTNLISLPNTI-----YLLRSLKNLLLSGCSIFGMFPDKWNPTIQP 857

Query: 661 ACSFSKMMETTSWSSEFPH 680
            CS SKMMET  WS + PH
Sbjct: 890 VCSPSKMMETALWSLKVPH 857

BLAST of MELO3C004303T1 vs. TrEMBL
Match: A0A0A0LJM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022270 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.9e-06
Identity = 76/301 (25.25%), Postives = 132/301 (43.85%), Query Frame = 1

Query: 319 KAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSDSLKWIKWHGFAHRSL 378
           K   L+L   + L   P+ +  + +L+ L +   +   KI  LS +      H +   +L
Sbjct: 648 KLTVLNLDGCSNLKKLPRGYFMLSSLKKLNLSYCKKLEKIPDLSSASNLTSLHIYECTNL 707

Query: 379 PSCFITKSLVGLD-MQHSFIKKFGKRLEV-NYSSTGCLLDWRLKHVNLSYSSLLEQIPHF 438
               I +S+  LD ++  ++K+    +++ +Y S   LL      ++LS    LE  P  
Sbjct: 708 R--VIHESVGSLDKLEGLYLKQCTNLVKLPSYLSLKSLLC-----LSLSGCCKLESFPTI 767

Query: 439 PAASNLEELHLS--ECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLK 498
             A N++ L     + T ++++  S+  L KL  L L+GC+NL  LP + +++RSLK+L 
Sbjct: 768 --AENMKSLRTLNLDFTAIKELPSSIRYLTKLWTLKLNGCTNLISLPNTIYLLRSLKNLL 827

Query: 499 LSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSH 558
           LS C      PD              C+  +M+  ++ SL     LV    F      SH
Sbjct: 828 LSGCSIFGMFPD-----KWNPTIQPVCSPSKMMETALWSLKVPHFLVPNESF------SH 887

Query: 559 LHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNING 616
           + L  LQ   +S    L+    +A  L  ++L +  F+++   P        LS L +  
Sbjct: 888 ITLLDLQSCNISNANFLDILCDVAPFLSDLRLSENKFSSL---PSCLHKFMSLSNLELRN 925


HSP 2 Score: 882.1 bits (2278), Expect = 4.3e-253
Identity = 462/654 (70.64%), Postives = 532/654 (81.35%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVGIYGIGG+GKTTLAKALYNKI+SQFEGCCFLSNVR+ASKQFNGL QLQE+LLYEIL  
Sbjct: 230 MVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILMV 289

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLK VNLDRGI IIR+RL SKKVLIVLDDVDKLEQLEALVGG DWFG+GS+IIVTTRN+H
Sbjct: 290 DLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFGKGSRIIVTTRNKH 349

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LL SHGFDE+HNI GL +DKAI+LFSWHAFK++ PSSNYL L +RATSYCKGHPLALVVL
Sbjct: 350 LLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRATSYCKGHPLALVVL 409

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
           GSFLC RDQ EW SILDEFENSL+ +IKDILQLSFDGLED++KDIFLDISCLLVGE+V Y
Sbjct: 410 GSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFLDISCLLVGEKVEY 469

Query: 241 VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
           VK++L ACH+N+DFG+I+LMDLSLIT+EN  VQMHDLI+QMG KIV GES E GKRSRLW
Sbjct: 470 VKDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVCGESLELGKRSRLW 529

Query: 301 LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
           L QD+WEV VNNSGTD IKAIKLD  NPTRL V+ QAFR MKNLR+LIVQNARFSTKI++
Sbjct: 530 LVQDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLLIVQNARFSTKIEY 589

Query: 361 LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLK 420
           L DSLKWIKWHGF   +LPSCFITK+LVGLD+Q+SF+K FGKRLE       C    RLK
Sbjct: 590 LPDSLKWIKWHGFPQPTLPSCFITKNLVGLDLQYSFMKTFGKRLE------DCK---RLK 649

Query: 421 HVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKL 480
           HV+LS+S+ LE+IP+F AASNLEEL+L  C NL  IDKSVFSLDKLTILNL GCSNLKKL
Sbjct: 650 HVDLSHSTFLEKIPNFSAASNLEELYLINCKNLGMIDKSVFSLDKLTILNLAGCSNLKKL 709

Query: 481 PTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTL 540
           P  YF++RSL++L LS+CKKLE+IPD S ASNLE LYL +CTNLRMI +S+ SL KL  L
Sbjct: 710 PRGYFILRSLRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMIDKSVFSLHKLTIL 769

Query: 541 VLRRCFNLAKLP-SHLHLKSLQYLGLSGCRKLENFPTI--AENLKSIKLLDLDFTAIKEV 600
            L  C NL KLP S+  L SLQYL LS C+KLE  P +  A NL+S+ L +   T ++ +
Sbjct: 770 NLDVCSNLKKLPTSYYKLWSLQYLNLSYCKKLEKIPDLSAASNLQSLCLHEC--TNLRLI 829

Query: 601 PPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVP 652
             S G L +L  ++++GCTN    P  +       L+SL  + L  C + E  P
Sbjct: 830 HESVGSLYKLIDMDLSGCTNLAKLPTYL------RLKSLRYLGLSECCKLESFP 866

BLAST of MELO3C004303T1 vs. TrEMBL
Match: E5GB33_CUCME (TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.1e-94
Identity = 178/261 (68.20%), Postives = 213/261 (81.61%), Query Frame = 1

Query: 419 LKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLK 478
           L+++NLS+   LE+IP F AASNLEEL+L  CTNLR IDKSVFSL KLTILNLD CSNLK
Sbjct: 710 LRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMIDKSVFSLHKLTILNLDVCSNLK 769

Query: 479 KLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLV 538
           KLPTSY+ + SL++L LSYCKKLE+IPDLS ASNL+SL L +CTNLR+IHES+GSL KL+
Sbjct: 770 KLPTSYYKLWSLQYLNLSYCKKLEKIPDLSAASNLQSLCLHECTNLRLIHESVGSLYKLI 829

Query: 539 TLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVP 598
            + L  C NLAKLP++L LKSL+YLGLS C KLE+FP+IAEN++S++ LD+DFTAIKE+P
Sbjct: 830 DMDLSGCTNLAKLPTYLRLKSLRYLGLSECCKLESFPSIAENMESLRELDMDFTAIKELP 889

Query: 599 PSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 658
            S GYLTQL RLN+ GCTN IS PNTI     YLLR+L+ + L GCSRFE+ PH+W   I
Sbjct: 890 SSIGYLTQLYRLNLTGCTNLISLPNTI-----YLLRNLDKLLLSGCSRFEMFPHKWDPTI 949

Query: 659 QPACSFSKMMETTSWSSEFPH 680
           QP CS SKMME TSWS E+PH
Sbjct: 950 QPVCSPSKMMEATSWSLEYPH 965


HSP 2 Score: 131.3 bits (329), Expect = 4.3e-27
Identity = 100/260 (38.46%), Postives = 144/260 (55.38%), Query Frame = 1

Query: 323 LDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSDSLKWIKWHGFAHRSL---- 382
           L+LA  + L   P+ +  +++LR L + + +   KI   S +    + + F   +L    
Sbjct: 689 LNLAGCSNLKKLPRGYFILRSLRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTNLRMID 748

Query: 383 PSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPA 442
            S F    L  L++    +    K+L  +Y        W L+++NLSY   LE+IP   A
Sbjct: 749 KSVFSLHKLTILNLD---VCSNLKKLPTSYYKL-----WSLQYLNLSYCKKLEKIPDLSA 808

Query: 443 ASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYC 502
           ASNL+ L L ECTNLR I +SV SL KL  ++L GC+NL KLPT Y  ++SL++L LS C
Sbjct: 809 ASNLQSLCLHECTNLRLIHESVGSLYKLIDMDLSGCTNLAKLPT-YLRLKSLRYLGLSEC 868

Query: 503 KKLERIPDLSLASNLESL--YLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLH 562
            KLE  P  S+A N+ESL     D T ++ +  SIG L +L  L L  C NL  LP+ ++
Sbjct: 869 CKLESFP--SIAENMESLRELDMDFTAIKELPSSIGYLTQLYRLNLTGCTNLISLPNTIY 928

Query: 563 -LKSLQYLGLSGCRKLENFP 576
            L++L  L LSGC + E FP
Sbjct: 929 LLRNLDKLLLSGCSRFEMFP 937


HSP 3 Score: 59.7 bits (143), Expect = 1.6e-05
Identity = 61/242 (25.21%), Postives = 100/242 (41.32%), Query Frame = 1

Query: 419  LKHVNLSYSSLLEQIPHFPAASNLEELHLSEC--TNLRKIDKSVFSLDKLTILNLDGCSN 478
            L+++ LS    LE  P    A N+E L   +   T ++++  S+  L +L  LNL GC+N
Sbjct: 851  LRYLGLSECCKLESFPSI--AENMESLRELDMDFTAIKELPSSIGYLTQLYRLNLTGCTN 910

Query: 479  LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 538
            L  LP + +++R+L  L LS C + E  P        +      C+  +M+  +  SL+ 
Sbjct: 911  LISLPNTIYLLRNLDKLLLSGCSRFEMFP-----HKWDPTIQPVCSPSKMMEATSWSLE- 970

Query: 539  LVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKE 598
                          L SH  L  LQ   +S  + LE    +A  L  ++L +  F+++  
Sbjct: 971  -----YPHLLPNESLCSHFTLLDLQSCNISNAKFLEILCDVAPFLSDLRLSENKFSSL-- 1030

Query: 599  VPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQ 658
             P        L  L +  C      PN        L ++++N+   GC      P     
Sbjct: 1031 -PSCLHKFMSLWNLELKNCKFLQEIPN--------LPQNIQNLDASGCKSLARSPDNIMD 1068


HSP 4 Score: 842.0 bits (2174), Expect = 5.0e-241
Identity = 438/653 (67.08%), Postives = 521/653 (79.79%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           M+GIYGIGG+GKTTLAKALYNKI++QFEG CFLSNVR+ SKQFNGLVQLQE LLYEILK 
Sbjct: 221 MMGIYGIGGIGKTTLAKALYNKIANQFEGFCFLSNVRETSKQFNGLVQLQEKLLYEILKF 280

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLK  NLD GI IIRSRL SKKVLIVLDDVDKL+QLEALVG RDWFG GSKIIVTTRN H
Sbjct: 281 DLKIGNLDEGINIIRSRLRSKKVLIVLDDVDKLKQLEALVGERDWFGHGSKIIVTTRNSH 340

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSH FDE + +R L    +++LFSWHAFK+SHPSSNYL L +RAT+YCKGHPLALVVL
Sbjct: 341 LLSSHEFDEKYGVRELSHGHSLELFSWHAFKKSHPSSNYLDLSKRATNYCKGHPLALVVL 400

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
           GSFLCTRDQ +W +ILDEFENSLS +I+ I+Q+SFDGLE+++K+IFLDISCL VGE+VNY
Sbjct: 401 GSFLCTRDQIKWRTILDEFENSLSEDIEHIIQISFDGLEEKIKEIFLDISCLFVGEKVNY 460

Query: 241 VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
           VK++L+ CH ++DFGII+LMDLSLITVEN  VQMHDLI+QMG KIV GES EPGKRSRLW
Sbjct: 461 VKSVLNTCHFSLDFGIIVLMDLSLITVENEEVQMHDLIRQMGQKIVNGESFEPGKRSRLW 520

Query: 301 LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
           L  D+ +VF +NSGT  +KAIKLDL+NPTRL VD +AFR+MKNLR+LIV+NARFST +++
Sbjct: 521 LVHDVLKVFADNSGTIAVKAIKLDLSNPTRLDVDSRAFRNMKNLRLLIVRNARFSTNVEY 580

Query: 361 LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLK 420
           L D+LKWIKWHGF+HR LP  F+ K+LVGLD++HS I+  GK  +       C    RLK
Sbjct: 581 LPDNLKWIKWHGFSHRFLPLSFLKKNLVGLDLRHSLIRNLGKGFK------DCK---RLK 640

Query: 421 HVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKL 480
           HV+LSYSSLLE+IP FPA SNLEEL+L+ CTNLR I KSV SL KL  L+LD CSNL KL
Sbjct: 641 HVDLSYSSLLEKIPDFPATSNLEELYLNNCTNLRTIPKSVVSLGKLLTLDLDHCSNLIKL 700

Query: 481 PTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTL 540
           P SY M++SLK LKL+YCKKLE++PD S ASNLE LYL++CTNLRMIH+SIGSL KLVTL
Sbjct: 701 P-SYLMLKSLKVLKLAYCKKLEKLPDFSTASNLEKLYLKECTNLRMIHDSIGSLSKLVTL 760

Query: 541 VLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFP--TIAENLKSIKLLDLDFTAIKEVP 600
            L +C NL KLPS+L LKSL+YL L+ C+KLE  P  + A NLKS+ L     T ++ + 
Sbjct: 761 DLGKCSNLEKLPSYLTLKSLEYLNLAHCKKLEEIPDFSSALNLKSLYLEQC--TNLRVIH 820

Query: 601 PSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVP 652
            S G L  L  L++  CTN    P+ +       L+SL +  L GC + E+ P
Sbjct: 821 ESIGSLNSLVTLDLRQCTNLEKLPSYL------KLKSLRHFELSGCHKLEMFP 855

BLAST of MELO3C004303T1 vs. TrEMBL
Match: A0A0A0LLK3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020890 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 6.0e-77
Identity = 157/261 (60.15%), Postives = 197/261 (75.48%), Query Frame = 1

Query: 419 LKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLK 478
           LK + L+Y   LE++P F  ASNLE+L+L ECTNLR I  S+ SL KL  L+L  CSNL+
Sbjct: 700 LKVLKLAYCKKLEKLPDFSTASNLEKLYLKECTNLRMIHDSIGSLSKLVTLDLGKCSNLE 759

Query: 479 KLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLV 538
           KLP SY  ++SL++L L++CKKLE IPD S A NL+SLYLE CTNLR+IHESIGSL+ LV
Sbjct: 760 KLP-SYLTLKSLEYLNLAHCKKLEEIPDFSSALNLKSLYLEQCTNLRVIHESIGSLNSLV 819

Query: 539 TLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVP 598
           TL LR+C NL KLPS+L LKSL++  LSGC KLE FP IAEN+KS+  L LD TAI+E+P
Sbjct: 820 TLDLRQCTNLEKLPSYLKLKSLRHFELSGCHKLEMFPKIAENMKSLISLHLDSTAIRELP 879

Query: 599 PSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 658
            S GYLT L  LN++GCTN IS P+TI     YLL+SL++++L GCSRF++   +W    
Sbjct: 880 SSIGYLTALLVLNLHGCTNLISLPSTI-----YLLKSLKHLYLGGCSRFQLFSRKWDPTA 939

Query: 659 QPACSFSKMMETTSWSSEFPH 680
            P CSFSK+M+T+S SSEFPH
Sbjct: 940 HPLCSFSKIMDTSS-SSEFPH 953


HSP 2 Score: 125.6 bits (314), Expect = 2.4e-25
Identity = 117/373 (31.37%), Postives = 180/373 (48.26%), Query Frame = 1

Query: 319  KAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSDSLKWIKWHGFAHRSL 378
            K + LDL + + L   P ++  +K+L+VL      +  K++ L D         F+  S 
Sbjct: 676  KLLTLDLDHCSNLIKLP-SYLMLKSLKVL---KLAYCKKLEKLPD---------FSTASN 735

Query: 379  PSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDW-------RLKHVNLSYSSLLE 438
                  K    L M H  I    K + ++      L           L+++NL++   LE
Sbjct: 736  LEKLYLKECTNLRMIHDSIGSLSKLVTLDLGKCSNLEKLPSYLTLKSLEYLNLAHCKKLE 795

Query: 439  QIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLK 498
            +IP F +A NL+ L+L +CTNLR I +S+ SL+ L  L+L  C+NL+KLP SY  ++SL+
Sbjct: 796  EIPDFSSALNLKSLYLEQCTNLRVIHESIGSLNSLVTLDLRQCTNLEKLP-SYLKLKSLR 855

Query: 499  HLKLSYCKKLERIPDLSLASNLESLYL--EDCTNLRMIHESIGSLDKLVTLVLRRCFNLA 558
            H +LS C KLE  P   +A N++SL     D T +R +  SIG L  L+ L L  C NL 
Sbjct: 856  HFELSGCHKLEMFP--KIAENMKSLISLHLDSTAIRELPSSIGYLTALLVLNLHGCTNLI 915

Query: 559  KLPSHLH-LKSLQYLGLSGCRKLENF-----PTIAENLKSIKLLDLDFTAIKEVP----P 618
             LPS ++ LKSL++L L GC + + F     PT A  L S   + +D ++  E P    P
Sbjct: 916  SLPSTIYLLKSLKHLYLGGCSRFQLFSRKWDPT-AHPLCSFSKI-MDTSSSSEFPHLLVP 975

Query: 619  STGYLTQLSRLNINGCT-----------NPISHPNTICLSR---------LYLLRSLENI 653
                 ++ + L++  C            N     ++I LS          L+   SL N+
Sbjct: 976  KESLCSKFTMLDLQCCNISNVDFLEILCNVAPFLSSILLSENKFSSLPPCLHKFMSLWNL 1030


HSP 3 Score: 67.4 bits (163), Expect = 7.7e-08
Identity = 66/213 (30.99%), Postives = 102/213 (47.89%), Query Frame = 1

Query: 4    IYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKDDLK 63
            I G+ G+ +TTLA ++ NK    +E    L + ++A     G +    N         L 
Sbjct: 1277 IRGMEGMAETTLANSICNK----YERSRNLFSAKKALNHSTGFLCGDGN--------GLS 1336

Query: 64   FVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRHLLS 123
            +  +DR   I+  RL S+K L + DD D+   L  +       G G++     R+R L  
Sbjct: 1337 WEMVDR--PILSDRLSSQKYLRIFDDRDRYGDLNDVA-----HGTGNRF----RSRFL-- 1396

Query: 124  SHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVLGSF 183
                  M +I+   +D   +   W      +   +YL L ++A  YCKG PL L VL SF
Sbjct: 1397 -----RMDDIK---EDDIREEPYW-----KYMERDYLDLSKQAVDYCKGLPLGLEVLDSF 1451

Query: 184  LCTRDQTEWISILDEFE-NSLSNNIKDILQLSF 216
            L  RDQ++  S+LD +E +SL   I++ILQ+ F
Sbjct: 1457 LYNRDQSKCKSVLDSYEKSSLHKRIQEILQIKF 1451


HSP 4 Score: 745.3 bits (1923), Expect = 6.3e-212
Identity = 402/657 (61.19%), Postives = 490/657 (74.58%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           M+GIYGIGG+GKTTLAKALY+K++SQFEGCC+L +VR+ASK F+GL QLQ+ LL++ILK 
Sbjct: 217 MLGIYGIGGIGKTTLAKALYDKMASQFEGCCYLRDVREASKLFDGLTQLQKKLLFQILKY 276

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DL+ V+LD GI II++RL SKKVLI+LDDVDKLEQL+ALVGG DWFGQG+KIIVTTRN+ 
Sbjct: 277 DLEVVDLDWGINIIKNRLRSKKVLILLDDVDKLEQLQALVGGHDWFGQGTKIIVTTRNKQ 336

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LL SHGFD+M+ ++GL + +AI+LF  HAFK   PSSNYL L ERAT YC GHPLAL+VL
Sbjct: 337 LLVSHGFDKMYEVQGLSKHEAIELFRRHAFKNLQPSSNYLDLSERATRYCTGHPLALIVL 396

Query: 181 GSFLCTR-DQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVN 240
           GSFLC R D  EW  ILD FENSL  +IKDILQLSFDGLED VK+IFLDISCLLVG+ V+
Sbjct: 397 GSFLCDRSDLAEWSGILDGFENSLRKDIKDILQLSFDGLEDEVKEIFLDISCLLVGKRVS 456

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGES-PEPGKRSR 300
           YVK +LS CH  +DFGI  L DLSLI  E+  VQMHDLI+QMGHKIV+ ES  +PGKRSR
Sbjct: 457 YVKKMLSECHSILDFGITKLKDLSLIRFEDDRVQMHDLIKQMGHKIVHDESHDQPGKRSR 516

Query: 301 LWLAQDIWEVFVNNSGTDTIKAIKLDLANPTR-LHVDPQAFRSMKNLRVLIVQ-NARFST 360
           LWL +DI EVF NNSG+D +KAIKL L +P R + +DP+AFRSMKNLR+L+V  N RF  
Sbjct: 517 LWLEKDILEVFSNNSGSDAVKAIKLVLTDPKRVIDLDPEAFRSMKNLRILMVDGNVRFCK 576

Query: 361 KIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLD 420
           KIK+L + LKWIKWH FAH SLPSCFITK LVGLD+QHSFI  FGK L+       C+  
Sbjct: 577 KIKYLPNGLKWIKWHRFAHPSLPSCFITKDLVGLDLQHSFITNFGKGLQ------NCM-- 636

Query: 421 WRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSN 480
            RLK ++L +S +L++I    AA NLEEL+LS C+NL+ I KS  SL KL  L+L  C N
Sbjct: 637 -RLKLLDLRHSVILKKISESSAAPNLEELYLSNCSNLKTIPKSFLSLRKLVTLDLHHCVN 696

Query: 481 LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 540
           LKK+P SY    +L+ L LS+CKKLE+IPD+S ASNL SL  E CTNL MIH+SIGSL K
Sbjct: 697 LKKIPRSYISWEALEDLDLSHCKKLEKIPDISSASNLRSLSFEQCTNLVMIHDSIGSLTK 756

Query: 541 LVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIA--ENLKSIKLLDLDFTAI 600
           LVTL L+ C NL KLP ++    LQ L LS C+KLE  P  +   NLK + L     T++
Sbjct: 757 LVTLKLQNCSNLKKLPRYISWNFLQDLNLSWCKKLEEIPDFSSTSNLKHLSLEQC--TSL 816

Query: 601 KEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVP 652
           + V  S G L++L  LN+  C+N    P+ +       L+SL+N+ L GC + E  P
Sbjct: 817 RVVHDSIGSLSKLVSLNLEKCSNLEKLPSYL------KLKSLQNLTLSGCCKLETFP 856

BLAST of MELO3C004303T1 vs. TAIR10
Match: AT5G36930.2 (AT5G36930.2 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 366.7 bits (940), Expect = 3.1e-101
Identity = 238/640 (37.19%), Postives = 341/640 (53.28%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILK- 60
           ++ IYG+GG+GKTTLAK  +N+ S  FEG  FL N R+ SK+  G   LQ  LL +IL+ 
Sbjct: 214 VIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRR 273

Query: 61  DDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNR 120
           +D++F  LD  +   + R  SK+VL+V+DDVD + QL +    RD FG GS+II+TTRN 
Sbjct: 274 NDIEFKGLDHAV---KERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNM 333

Query: 121 HLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVV 180
           HLL     +  ++ + L  D++++LFSWHAF+ S P   +L   E   +YC G PLA+ V
Sbjct: 334 HLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEV 393

Query: 181 LGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVN 240
           LG+FL  R   EW S L   +   ++NI+  LQ+SF+ L    KD+FLDI+C  +G +  
Sbjct: 394 LGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVDSY 453

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEP-GKRSR 300
           YV  IL  C+L  D  + +LM+  LIT+    + MHDL++ MG +IV   SP+  G+RSR
Sbjct: 454 YVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSR 513

Query: 301 LWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKI 360
           LW   D+  V    SGT+ I+ + L        + + +AF  M+ LR+L ++    +   
Sbjct: 514 LWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLNGSY 573

Query: 361 KHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWR 420
           +H    L+W+ WHGF+    P     +SL  LD+Q+S +K+F K       +        
Sbjct: 574 EHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQSPPQPAN------M 633

Query: 421 LKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLD-KLTILNLDGCSNL 480
           +K+++LS+S  L + P F    N+E+L L  C +L  + KS+  LD KL +LNL  C  L
Sbjct: 634 VKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIEL 693

Query: 481 KKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESL--YLEDCTNLRMIHESIGSLD 540
             LP   + ++SL+ L LS C KLER+ D      LESL   L D T LR I  +I  L 
Sbjct: 694 DVLPEEIYKLKSLESLFLSNCSKLERLDD--ALGELESLTTLLADFTALREIPSTINQLK 753

Query: 541 KLVTLVLRRC--------FNLAKLPSH-------LHLKSLQY---LGLSGCR-KLENFPT 600
           KL  L L  C         NL    SH       + L  L Y   L L  C    E  P 
Sbjct: 754 KLKRLSLNGCKGLLSDDIDNLYSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPE 813

Query: 601 IAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCT 617
              +L  ++ LDL   +   +P     L  L  L ++ C+
Sbjct: 814 DIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDCS 842


HSP 2 Score: 41.6 bits (96), Expect = 2.3e-03
Identity = 64/248 (25.81%), Postives = 99/248 (39.92%), Query Frame = 1

Query: 319 KAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSD------SLKWIKWHG 378
           K + L+L++   L V P+    +K+L  L + N    +K++ L D      SL  +    
Sbjct: 672 KLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNC---SKLERLDDALGELESLTTLLADF 731

Query: 379 FAHRSLPSCFIT------------KSLVGLDMQHSFIKK---FGKRLEVNYSSTGCLLDW 438
            A R +PS                K L+  D+ + + +K         V+ S    +   
Sbjct: 732 TALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSLLRPVSLSGLTYMRIL 791

Query: 439 RLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNL 498
            L + NLS   + E I       +L+    S C     +     +L  L  L L  CS L
Sbjct: 792 SLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCN----LPTDFATLPNLGELLLSDCSKL 851

Query: 499 KKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKL 546
           + + +   + RSL  L +  C  L+R PD+S  S L  L L DC +L  I   I + + L
Sbjct: 852 QSILS---LPRSLLFLDVGKCIMLKRTPDISKCSALFKLQLNDCISLFEI-PGIHNHEYL 908


HSP 3 Score: 37.7 bits (86), Expect = 3.3e-02
Identity = 37/139 (26.62%), Postives = 63/139 (45.32%), Query Frame = 1

Query: 520 DCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAE 579
           +C  + +  ES+ +LD L    L+R +     P   ++  ++YL LS    L   P  + 
Sbjct: 588 ECFPINLSLESLAALD-LQYSNLKRFWKAQSPPQPANM--VKYLDLSHSVYLRETPDFSY 647

Query: 580 NLKSIKLLDLDFTAIKEVPPSTGYLTQ-LSRLNINGCTNPISHPNTICLSRLYLLRSLEN 639
                KL+ ++  ++  V  S G L + L  LN++ C      P       +Y L+SLE+
Sbjct: 648 FPNVEKLILINCKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPE-----EIYKLKSLES 707

Query: 640 IFLRGCSRFEIVPHEWGQI 658
           +FL  CS+ E +    G++
Sbjct: 708 LFLSNCSKLERLDDALGEL 718

BLAST of MELO3C004303T1 vs. TAIR10
Match: AT5G17680.1 (AT5G17680.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 365.5 bits (937), Expect = 6.9e-101
Identity = 245/685 (35.77%), Postives = 380/685 (55.47%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           M+GI+G+GG+GKTT+AK LYN++S QF+  CF+ NV++   ++ G+ +LQ   L  + ++
Sbjct: 208 MLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRY-GVRRLQVEFLCRMFQE 267

Query: 61  -DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNR 120
            D +  +      II+ R   K V IVLDDVD+ EQL  LV    WFG GS+IIVTTR+R
Sbjct: 268 RDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDR 327

Query: 121 HLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSN-YLGLVERATSYCKGHPLALV 180
           HLL SHG + ++ ++ L + +A++LF  +AF+E     + +  L  +A +Y  G PLAL 
Sbjct: 328 HLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALR 387

Query: 181 VLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEV 240
           VLGSFL  R Q EW S L   +    ++I ++L++S+DGL+++ K IFL ISC    ++V
Sbjct: 388 VLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQV 447

Query: 241 NYVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGES-PEPGKRS 300
           +YV+ +L  C    + GI IL + SLI   NG V++HDL++QMG ++V  ++   P +R 
Sbjct: 448 DYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRL 507

Query: 301 RLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTK 360
            LW  +DI  +   NSGT  ++ I L+L+  + +    +AF  + NL++L   +  F  +
Sbjct: 508 LLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGE 567

Query: 361 --------IKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYS 420
                   + +L   L++++W G+  +++PS F  + LV L M +S ++K    ++   +
Sbjct: 568 TRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRN 627

Query: 421 STGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTIL 480
                    LK ++LS    L ++P    A+NLEEL+LS C +L ++  S+ +L  L+  
Sbjct: 628 ---------LKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCF 687

Query: 481 NLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHE 540
            L  C  LK +P    +++SL+ + +S C  L+  P++S   N   LYL   T +  +  
Sbjct: 688 YLTNCIQLKDIPIG-IILKSLETVGMSGCSSLKHFPEISW--NTRRLYLSS-TKIEELPS 747

Query: 541 SIGSLDKLVTLVLRRCFNLAKLPSHL-HLKSLQYLGLSGCRKLENFPTIAENLKSIKLLD 600
           SI  L  LV L +  C  L  LPS+L HL SL+ L L GCR+LEN P   +NL S++ L+
Sbjct: 748 SISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLE 807

Query: 601 LD-FTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTIC-LSRLYL-------------- 654
           +     + E P  +   T +  L I+  T+    P  IC LS+L                
Sbjct: 808 VSGCLNVNEFPRVS---TSIEVLRISE-TSIEEIPARICNLSQLRSLDISENKRLASLPV 867


HSP 2 Score: 93.6 bits (231), Expect = 5.1e-19
Identity = 67/198 (33.84%), Postives = 104/198 (52.53%), Query Frame = 1

Query: 415 LDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGC 474
           + W  + + LS + + E        S L +L +S+C  LR +   +  L  L  LNLDGC
Sbjct: 714 ISWNTRRLYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGC 773

Query: 475 SNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSL 534
             L+ LP +   + SL+ L++S C  +   P +S  +++E L + + T++  I   I +L
Sbjct: 774 RRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVS--TSIEVLRISE-TSIEEIPARICNL 833

Query: 535 DKLVTLVLRRCFNLAKLP-SHLHLKSLQYLGLSGCRKLENFP-TIAENLKSIKLLDLDFT 594
            +L +L +     LA LP S   L+SL+ L LSGC  LE+FP  I + +  ++  DLD T
Sbjct: 834 SQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSCLRWFDLDRT 893

Query: 595 AIKEVPPSTGYLTQLSRL 611
           +IKE+P + G L  L  L
Sbjct: 894 SIKELPENIGNLVALEVL 908


HSP 3 Score: 67.4 bits (163), Expect = 3.9e-11
Identity = 97/352 (27.56%), Postives = 143/352 (40.62%), Query Frame = 1

Query: 355  STKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCL 414
            STKI+ L  S+  +           SC     LV LDM         +RL    S  G L
Sbjct: 725  STKIEELPSSISRL-----------SC-----LVKLDMSDC------QRLRTLPSYLGHL 784

Query: 415  LDWRLKHVNLSYSSLLEQIP---------------------HFPAAS-NLEELHLSECTN 474
            +   LK +NL     LE +P                      FP  S ++E L +SE T+
Sbjct: 785  VS--LKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISE-TS 844

Query: 475  LRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASN 534
            + +I   + +L +L  L++     L  LP S   +RSL+ LKLS C  LE  P L +   
Sbjct: 845  IEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFP-LEICQT 904

Query: 535  LESL--YLEDCTNLRMIHESIGSLDKLVTL-----VLRRC-FNLAKL------------- 594
            +  L  +  D T+++ + E+IG+L  L  L     V+RR  +++A+L             
Sbjct: 905  MSCLRWFDLDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFF 964

Query: 595  ----------PSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPST 647
                      P       L+ L LS     E  P    NL ++  LDL     + +P S 
Sbjct: 965  TPEGLLHSLCPPLSRFDDLRALSLSNMNMTE-IPNSIGNLWNLLELDLSGNNFEFIPASI 1024


HSP 4 Score: 43.9 bits (102), Expect = 4.6e-04
Identity = 39/128 (30.47%), Postives = 60/128 (46.88%), Query Frame = 1

Query: 434  PHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHL 493
            P      +L  L LS   N+ +I  S+ +L  L  L+L G +N + +P S   +  L  L
Sbjct: 949  PPLSRFDDLRALSLSNM-NMTEIPNSIGNLWNLLELDLSG-NNFEFIPASIKRLTRLNRL 1008

Query: 494  KLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNL---AK 553
             L+ C++L+ +PD  L   L  +Y+  CT+L  I         L  LV   C+ L   A+
Sbjct: 1009 NLNNCQRLQALPD-ELPRGLLYIYIHSCTSLVSISGCFNQY-CLRKLVASNCYKLDQAAQ 1068

Query: 554  LPSHLHLK 559
            +  H +LK
Sbjct: 1069 ILIHRNLK 1072

BLAST of MELO3C004303T1 vs. TAIR10
Match: AT4G12010.1 (AT4G12010.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 337.4 bits (864), Expect = 2.0e-92
Identity = 231/682 (33.87%), Postives = 369/682 (54.11%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           ++GI G+ G+GKTTLA  LY ++  QF+G CFL+N+R+ S + +GL  L + L   +L D
Sbjct: 211 IIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFLTNIRENSGR-SGLESLLQKLFSTVLND 270

Query: 61  -DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNR 120
            DL+             RL SK++LIVLDDV+  +Q+  L+G   W+  GS+II+TTR+ 
Sbjct: 271 RDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDS 330

Query: 121 HLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVV 180
            L+ +      + +  L   +A+KLFS +AF  S P   + GL      Y KGHPLAL V
Sbjct: 331 KLIETIK-GRKYVLPKLNDREALKLFSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKV 390

Query: 181 LGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVN 240
           LGS LC RD   W + LD  ++    +I ++L+ S++ L    K++FLDI+C    E V+
Sbjct: 391 LGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLETSYEELTTEQKNVFLDIACFFRSENVD 450

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKI-------------- 300
           YV ++L++  ++V   +  L+D  LIT+ +  ++MHD++Q M  +I              
Sbjct: 451 YVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRIEMHDMLQTMAKEISLKVETIGIRDCRW 510

Query: 301 VYGESPEPGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLR 360
           +     +     RLW ++DI ++     GTD I+ I LD +    + +  +AF+ M NL+
Sbjct: 511 LSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLK 570

Query: 361 VLIVQNARFS------------TKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQ 420
            L + ++  S              +  L + L ++ WHG+  +S+P  F  K+LV L + 
Sbjct: 571 YLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLP 630

Query: 421 HSFIKKFGKRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNL 480
           HS +++       +    G      LK V+LS+S  L Q      A NLE L+L  CT+L
Sbjct: 631 HSQLEEIWD----DEKDVG-----MLKWVDLSHSINLRQCLGLANAHNLERLNLEGCTSL 690

Query: 481 RKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNL 540
           +K+  ++  L+KL  LNL  C++L+ LP      +SL+ L LS C  L++ P +S   N+
Sbjct: 691 KKLPSTINCLEKLIYLNLRDCTSLRSLPKG-IKTQSLQTLILSGCSSLKKFPLIS--ENV 750

Query: 541 ESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLH-LKSLQYLGLSGCRKLE 600
           E L L D T ++ + ESI +  +L  L L+ C  L  L S L+ LK LQ L LSGC +LE
Sbjct: 751 EVLLL-DGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELILSGCSQLE 810

Query: 601 NFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPIS-----HPNTICL 650
            FP I E+++S+++L +D T+I E+P    +L+ +   ++ G ++ +S      P T+  
Sbjct: 811 VFPEIKEDMESLEILLMDDTSITEMPKMM-HLSNIKTFSLCGTSSHVSVSMFFMPPTLGC 870


HSP 2 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 50/190 (26.32%), Postives = 85/190 (44.74%), Query Frame = 1

Query: 436 FPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKL 495
           FP  S   E+ L + T ++ + +S+ +  +L +LNL  C  LK L +  + ++ L+ L L
Sbjct: 729 FPLISENVEVLLLDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYKLKCLQELIL 788

Query: 496 SYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHL 555
           S C +LE  P++    ++ESL +     L M   SI  + K++ L   + F+L    SH+
Sbjct: 789 SGCSQLEVFPEIK--EDMESLEI-----LLMDDTSITEMPKMMHLSNIKTFSLCGTSSHV 848

Query: 556 HLKSLQYLGLSGCRKLEN----------FPTIAENLKSIKLLDLDFTAIKEVPPSTGYLT 615
            +         GC +L +           P     L S++ L L    I+ +P S   L 
Sbjct: 849 SVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGNNIENLPESFNQLN 908

BLAST of MELO3C004303T1 vs. TAIR10
Match: AT5G46450.1 (AT5G46450.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-89
Identity = 222/704 (31.53%), Postives = 358/704 (50.85%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNV----------RQASKQFNGLVQLQ 60
           MVGI+G  G+GKTT+A+AL++ +SSQF+   ++             R     +N  ++L+
Sbjct: 209 MVGIWGTSGIGKTTIARALFSNLSSQFQSSVYIDRAFISKSMEGYGRANPDDYNMKLRLR 268

Query: 61  ENLLYEIL-KDDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQG 120
           EN L+EIL K ++K       I  +  RL  +KVLI++DD+D  + L+ALVG   WFG G
Sbjct: 269 ENFLFEILGKKNMK-------IGAMEERLKHQKVLIIIDDLDDQDVLDALVGRTQWFGSG 328

Query: 121 SKIIVTTRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSY 180
           S+IIV T+N+H L +HG D ++      ++ A+++F  +AF+++ P   ++ L       
Sbjct: 329 SRIIVVTKNKHFLRAHGIDHVYEACLPSEELALEMFCRYAFRKNSPPDGFMELSSEVALR 388

Query: 181 CKGHPLALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKD-IFLD 240
               PL L VLGS+L  RD  +W+ ++   +N L   I+  L++S+DGL ++  + IF  
Sbjct: 389 AGNLPLGLKVLGSYLRGRDIEDWMDMMPRLQNDLDGKIEKTLRVSYDGLNNKKDEAIFRH 448

Query: 241 ISCLLVGEEVNYVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYG 300
           I+CL  GE+VN +K +L+   L+V+ G+  L+D SLI V   T++MH L+Q MG +IV  
Sbjct: 449 IACLFNGEKVNDIKLLLAESDLDVNIGLKNLVDKSLIFVREDTIEMHRLLQDMGKEIVRA 508

Query: 301 ESPEPGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLI 360
           +S EPG+R  L  ++ I++V  +N+GT  +  I LD+     L++   AF+ M+NL  L 
Sbjct: 509 QSNEPGEREFLVDSKHIYDVLEDNTGTKKVLGIALDINETDGLYIHESAFKGMRNLLFLN 568

Query: 361 VQNAR-------FSTKIKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFG 420
               +        S    HL   L+ + W  +  R +PS F  ++LV L M  S ++K  
Sbjct: 569 FYTKQKKDVTWHLSEGFDHLPPKLRLLSWEKYPLRCMPSNFRPENLVKLQMCESKLEKLW 628

Query: 421 KRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVF 480
             +   +S TG      L++++L  S  L++IP    A+NL++L +S CT+L ++  ++ 
Sbjct: 629 DGV---HSLTG------LRNMDLRGSENLKEIPDLSLATNLKKLDVSNCTSLVELSSTIQ 688

Query: 481 SLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDC 540
           +L++L  L ++ C NL+ LP     + SL  L L+ C KL   PD+S  + +  LYL + 
Sbjct: 689 NLNQLEELQMERCENLENLPIG-INLESLYCLNLNGCSKLRSFPDIS--TTISELYLSET 748

Query: 541 TNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAE-- 600
                                     + + P+ LHL++L YLGL   +  + +  +    
Sbjct: 749 A-------------------------IEEFPTELHLENLYYLGLYDMKSEKLWKRVQPLT 808

Query: 601 ------NLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLL 660
                 +    KL   D  ++ E+P S   L  L  LNI  CTN  + P  +       L
Sbjct: 809 PLMTMLSPSLTKLFLSDIPSLVELPSSFQNLHNLEHLNIARCTNLETLPTGV------NL 862

Query: 661 RSLENIFLRGCSRFEIVPHEWGQIIQPACSFSKMMETTSWSSEF 678
             LE +   GCSR    P     I       + + E   W  +F
Sbjct: 869 ELLEQLDFSGCSRLRSFPDISTNIFSLVLDGTGIEEVPWWIEDF 862


HSP 2 Score: 43.9 bits (102), Expect = 4.6e-04
Identity = 36/119 (30.25%), Postives = 54/119 (45.38%), Query Frame = 1

Query: 428 SLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMV 487
           SL+E    F    NLE L+++ CTNL  +   V +L+ L  L+  GCS L+  P     +
Sbjct: 784 SLVELPSSFQNLHNLEHLNIARCTNLETLPTGV-NLELLEQLDFSGCSRLRSFPDISTNI 843

Query: 488 RSLKHLKLSYCKKLERIP-DLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRC 546
            SL    +     +E +P  +     L  L +  C NL+ +  +I  L+KL T+    C
Sbjct: 844 FSL----VLDGTGIEEVPWWIEDFYRLSFLSMIGCNNLQGVSLNISKLEKLETVDFSDC 897


HSP 3 Score: 42.4 bits (98), Expect = 1.3e-03
Identity = 41/167 (24.55%), Postives = 70/167 (41.92%), Query Frame = 1

Query: 433 IPHFPAASNLEELHLSECTNLR--KIDKSVFSLD--------KLTILNLDGCSNLKKLPT 492
           I  FP   +LE L+     +++  K+ K V  L          LT L L    +L +LP+
Sbjct: 731 IEEFPTELHLENLYYLGLYDMKSEKLWKRVQPLTPLMTMLSPSLTKLFLSDIPSLVELPS 790

Query: 493 SYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVL 552
           S+  + +L+HL ++ C  LE +P       LE L    C+ LR   +   ++  LV    
Sbjct: 791 SFQNLHNLEHLNIARCTNLETLPTGVNLELLEQLDFSGCSRLRSFPDISTNIFSLVL--- 850

Query: 553 RRCFNLAKLPSHLH-LKSLQYLGLSGCRKLENFPTIAENLKSIKLLD 589
                + ++P  +     L +L + GC  L+        L+ ++ +D
Sbjct: 851 -DGTGIEEVPWWIEDFYRLSFLSMIGCNNLQGVSLNISKLEKLETVD 893

BLAST of MELO3C004303T1 vs. TAIR10
Match: AT5G18360.1 (AT5G18360.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 325.5 bits (833), Expect = 7.9e-89
Identity = 226/670 (33.73%), Postives = 352/670 (52.54%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQ-----FNGLVQLQENLLY 60
           MVGI+G  G+GKTT+A+AL+N++S  F+   F+ NV+ +S+      +   ++LQE  L 
Sbjct: 209 MVGIWGPAGIGKTTIARALFNRLSENFQHTIFMENVKGSSRTSELDAYGFQLRLQEQFLS 268

Query: 61  EILKDDLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVT 120
           E++  D K + +   + +++ RL   KVL+VLDDVDKLEQL+ALV    WFG GS+IIVT
Sbjct: 269 EVI--DHKHMKI-HDLGLVKERLQDLKVLVVLDDVDKLEQLDALVKQSQWFGSGSRIIVT 328

Query: 121 TRNRHLLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPL 180
           T N+ LL +HG   ++ +    +  ++++F  +AF ES      + L    T      PL
Sbjct: 329 TENKQLLRAHGITCIYELGFPSRSDSLQIFCQYAFGESSAPDGCIELATEITKLAGYLPL 388

Query: 181 ALVVLGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVG 240
           AL VLGS L    + E  S L     SL+ +I+++L++ +DG+ D+ K IFL I+CL  G
Sbjct: 389 ALKVLGSSLRGMSKDEQKSALPRLRTSLNEDIRNVLRVGYDGIHDKDKVIFLHIACLFNG 448

Query: 241 EEVNYVKNILSACHLNVDFGIIILMDLSLITVE--NGTVQMHDLIQQMGHKIVYGES-PE 300
           E V+YVK IL++  L+V FG+ +L   SLI +   N T+ MH+L++Q+G +IV  +S  E
Sbjct: 449 ENVDYVKQILASSGLDVTFGLQVLTSRSLIHISRCNRTITMHNLLEQLGREIVCEQSIAE 508

Query: 301 PGKRSRLWLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNA 360
           PGKR  L  A +I++V  +N+GT  +  I LD++    L ++ +AF  M NL  L    +
Sbjct: 509 PGKRQFLMDASEIYDVLADNTGTGAVLGISLDISKINELFLNERAFGGMHNLLFLRFYKS 568

Query: 361 RFSTK---------IKHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKR 420
             S           + +L   L+ + W  F   S+P  F  + LV ++++ S ++K  + 
Sbjct: 569 SSSKDQPELHLPRGLDYLPRKLRLLHWDAFPMTSMPLSFCPQFLVVINIRESQLEKLWEG 628

Query: 421 LEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSL 480
            +   S         LK ++LS S  L++IP    A N+EEL LS C +L  +  S+ +L
Sbjct: 629 TQPLRS---------LKQMDLSKSENLKEIPDLSKAVNIEELCLSYCGSLVMLPSSIKNL 688

Query: 481 DKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTN 540
           +KL +L++  CS L+ +P +   + SL  L L  C +LE  P++S               
Sbjct: 689 NKLVVLDMKYCSKLEIIPCN-MDLESLSILNLDGCSRLESFPEIS--------------- 748

Query: 541 LRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKS 600
                  IG L    T +      +A  P       L  L +SGC+ L+ FP +    K+
Sbjct: 749 -----SKIGFLSLSETAIEEIPTTVASWP------CLAALDMSGCKNLKTFPCLP---KT 808

Query: 601 IKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRG 654
           I+ LDL  T I+EVP     L++L++L +N C    S       S +  L  ++ +   G
Sbjct: 809 IEWLDLSRTEIEEVPLWIDKLSKLNKLLMNSCMKLRS-----ISSGISTLEHIKTLDFLG 831

BLAST of MELO3C004303T1 vs. NCBI nr
Match: gi|659071574|ref|XP_008460703.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 1022.7 bits (2643), Expect = 3.0e-295
Identity = 515/679 (75.85%), Postives = 581/679 (85.57%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVGIYGIGG+GKTTLAKALYNKI+SQFEGCCFLSNVR+ASKQFNGL QLQE+LLYEIL  
Sbjct: 231 MVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILTI 290

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
            LK VN DRGI IIR+RL SKKVLIVLDDVDKLEQLEALVGGRDWFGQGS+IIVTTRN+H
Sbjct: 291 YLKVVNFDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSRIIVTTRNKH 350

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSHGFDE+HNI GL ++KAI+LFSWHAFK++HPSSNYL L +RATSYC+GHPLALVVL
Sbjct: 351 LLSSHGFDEIHNILGLNEEKAIELFSWHAFKKNHPSSNYLNLSKRATSYCRGHPLALVVL 410

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
           GSFLCTRDQ EW SILDEFENSL+ +IKDILQLSFDGLED+VK IFLDISCLLVGE+V Y
Sbjct: 411 GSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKVKHIFLDISCLLVGEKVKY 470

Query: 241 VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
           VKN+LSACH+N+DFGII+LMDLSL+T+EN  VQMHDLI+QMGHKIV GES E GKRSRLW
Sbjct: 471 VKNMLSACHVNLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVCGESLELGKRSRLW 530

Query: 301 LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
           L QD+W+V VNNSGTD +KAIKLD  NPT+L VD QAFR MKNLR+LIVQNARF TKI++
Sbjct: 531 LVQDVWDVLVNNSGTDAVKAIKLDFPNPTKLDVDLQAFRKMKNLRLLIVQNARFCTKIEY 590

Query: 361 LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLK 420
           L DSLKWIKWHGF   +LPSCFITK+LVGLD+QHSFIK F KRL+       C    RLK
Sbjct: 591 LPDSLKWIKWHGFPQSTLPSCFITKNLVGLDLQHSFIKTFEKRLK------DC---ERLK 650

Query: 421 HVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKL 480
           HV+LSYS+LLEQIP F AASNL EL+L  CTNL  IDKS+FSL+ L +LNLDGCSNLKK 
Sbjct: 651 HVDLSYSTLLEQIPDFSAASNLGELYLINCTNLGMIDKSLFSLNNLIVLNLDGCSNLKKF 710

Query: 481 PTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTL 540
           P  YFM+ SLK L+LSYCKKLE+IPDLS ASNLE LYL++CTNLR+IHES+GSLDKL  L
Sbjct: 711 PRGYFMLSSLKELRLSYCKKLEKIPDLSAASNLERLYLQECTNLRLIHESVGSLDKLDHL 770

Query: 541 VLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPS 600
            LR+C NL+KLPSHL LKSLQ L LS C KLE+FPTI EN+KS++ LDLDFTAIKE+P S
Sbjct: 771 DLRQCTNLSKLPSHLRLKSLQNLELSRCCKLESFPTIDENMKSLRHLDLDFTAIKELPSS 830

Query: 601 TGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQIIQP 660
            GYLT+L  LN+  CTN IS PNTI     YLLR+L+ + L GCSRF I PH+W + IQP
Sbjct: 831 IGYLTELCTLNLTSCTNLISLPNTI-----YLLRNLDELLLSGCSRFRIFPHKWDRSIQP 890

Query: 661 ACSFSKMMETTSWSSEFPH 680
            CS +KM+ETTSWS EFPH
Sbjct: 891 VCSPTKMIETTSWSLEFPH 895

BLAST of MELO3C004303T1 vs. NCBI nr
Match: gi|659071574|ref|XP_008460703.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 53.1 bits (126), Expect = 2.2e-03
Identity = 60/242 (24.79%), Postives = 101/242 (41.74%), Query Frame = 1

Query: 419 LKHVNLSYSSLLEQIPHFPAASNLEEL-HLS-ECTNLRKIDKSVFSLDKLTILNLDGCSN 478
           L+++ LS    LE  P      N++ L HL  + T ++++  S+  L +L  LNL  C+N
Sbjct: 781 LQNLELSRCCKLESFPTID--ENMKSLRHLDLDFTAIKELPSSIGYLTELCTLNLTSCTN 840

Query: 479 LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 538
           L  LP + +++R+L  L LS C +    P        +      C+  +MI  +  SL+ 
Sbjct: 841 LISLPNTIYLLRNLDELLLSGCSRFRIFP-----HKWDRSIQPVCSPTKMIETTSWSLEF 900

Query: 539 LVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKE 598
              LV         L SH  L  L+   +S  + LE    +A  L  ++L +  F+++  
Sbjct: 901 PHLLVPNE-----SLFSHFTLLDLKSCNISNAKFLEILCDVAPFLSDLRLSENKFSSL-- 960

Query: 599 VPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQ 658
            P        L  L +  C      PN        L ++++ +   GC      P+    
Sbjct: 961 -PSCLHKFMSLWNLELKNCKFLQEIPN--------LPKNIQKMDASGCESLVRSPNNIVD 999


HSP 2 Score: 51.2 bits (121), Expect = 8.2e-03
Identity = 57/221 (25.79%), Postives = 97/221 (43.89%), Query Frame = 1

Query: 378 LPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLKHVNLSYSSLLEQIPHFP 437
           LPS    KSL  L++      +    ++ N  S        L+H++L ++++ E      
Sbjct: 772 LPSHLRLKSLQNLELSRCCKLESFPTIDENMKS--------LRHLDLDFTAIKELPSSIG 831

Query: 438 AASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSY 497
             + L  L+L+ CTNL  +  +++ L  L  L L GCS  +  P  +   RS++ +  S 
Sbjct: 832 YLTELCTLNLTSCTNLISLPNTIYLLRNLDELLLSGCSRFRIFPHKW--DRSIQPV-CSP 891

Query: 498 CKKLER-----------IPDLSLASNLESLYLEDC--TNLRMIHESIGSLDKLVTLVLRR 557
            K +E            +P+ SL S+   L L+ C  +N + +         L  L L  
Sbjct: 892 TKMIETTSWSLEFPHLLVPNESLFSHFTLLDLKSCNISNAKFLEILCDVAPFLSDLRLSE 951

Query: 558 CFNLAKLPSHLH-LKSLQYLGLSGCRKLENFPTIAENLKSI 585
               + LPS LH   SL  L L  C+ L+  P + +N++ +
Sbjct: 952 -NKFSSLPSCLHKFMSLWNLELKNCKFLQEIPNLPKNIQKM 980


HSP 3 Score: 1003.8 bits (2594), Expect = 1.4e-289
Identity = 511/679 (75.26%), Postives = 572/679 (84.24%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVG+YGIGG+GKTTLAKALYNKI+SQFEGCCFLSNVR+ASKQFNGL QLQE LLYEIL  
Sbjct: 230 MVGLYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQETLLYEILTV 289

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLK VNLDRGI IIR+RL  KKVLIVLDDVDKLEQLEALVGGRDWFGQGS+IIVTTRN+H
Sbjct: 290 DLKVVNLDRGINIIRNRLCLKKVLIVLDDVDKLEQLEALVGGRDWFGQGSRIIVTTRNKH 349

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSHGFDEM NI GL +DKAI+LFSWHAFK++HPSSNYL L +RATSYCKGHPLALVVL
Sbjct: 350 LLSSHGFDEMKNILGLDEDKAIELFSWHAFKKNHPSSNYLDLSKRATSYCKGHPLALVVL 409

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
           GSFLCTRDQ EW SILDEFENSL+ +IKDILQLSFDGLED+VKDIFLDISCLLVGE+  Y
Sbjct: 410 GSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKVKDIFLDISCLLVGEKGKY 469

Query: 241 VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
           VK++LSACH+N+DFGII+L DLS IT+ENG +QMHDLI+QMGHKIV GES E GKRSRLW
Sbjct: 470 VKDMLSACHVNLDFGIIVLTDLSFITIENGIMQMHDLIKQMGHKIVCGESLELGKRSRLW 529

Query: 301 LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
           L QD+WEV VNN GTD +K IKLD  N TRL VDPQAFR MKNLR+LIVQNARFSTKI++
Sbjct: 530 LVQDVWEVLVNNXGTDAVKGIKLDFPNSTRLDVDPQAFRKMKNLRLLIVQNARFSTKIEY 589

Query: 361 LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRLK 420
           L DSLKWIKWHGF   + PS F  K+LVGLD+QHSFIK FGKRLE       C    RLK
Sbjct: 590 LPDSLKWIKWHGFRQPTFPSFFTMKNLVGLDLQHSFIKTFGKRLE------DC---ERLK 649

Query: 421 HVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKKL 480
            V+LSYS+ LE+IP+F AASNLEEL+LS CTNL  IDKSVFSLDKLT+LNLDGCSNLKKL
Sbjct: 650 LVDLSYSTFLEKIPNFSAASNLEELYLSNCTNLGMIDKSVFSLDKLTVLNLDGCSNLKKL 709

Query: 481 PTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTL 540
           P  YFM+ SLK L LSYCKKLE+IPDLS ASNL SL++ +CTNLR+IHES+GSLDKL  L
Sbjct: 710 PRGYFMLSSLKKLNLSYCKKLEKIPDLSSASNLTSLHIYECTNLRVIHESVGSLDKLEGL 769

Query: 541 VLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPS 600
            L++C NL KLPS+L LKSL  L LSGC KLE+FPTIAEN+KS++ L+LDFTAIKE+P S
Sbjct: 770 YLKQCTNLVKLPSYLSLKSLLCLSLSGCCKLESFPTIAENMKSLRTLNLDFTAIKELPSS 829

Query: 601 TGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQIIQP 660
             YLT+L  L +NGCTN IS PNTI     YLLRSL+N+ L GCS F + P +W   IQP
Sbjct: 830 IRYLTKLWTLKLNGCTNLISLPNTI-----YLLRSLKNLLLSGCSIFGMFPDKWNPTIQP 889

Query: 661 ACSFSKMMETTSWSSEFPH 680
            CS SKMMET  WS + PH
Sbjct: 890 VCSPSKMMETALWSLKVPH 894

BLAST of MELO3C004303T1 vs. NCBI nr
Match: gi|778666836|ref|XP_011648823.1| (PREDICTED: LOW QUALITY PROTEIN: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 62.8 bits (151), Expect = 2.7e-06
Identity = 76/301 (25.25%), Postives = 132/301 (43.85%), Query Frame = 1

Query: 319 KAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKHLSDSLKWIKWHGFAHRSL 378
           K   L+L   + L   P+ +  + +L+ L +   +   KI  LS +      H +   +L
Sbjct: 685 KLTVLNLDGCSNLKKLPRGYFMLSSLKKLNLSYCKKLEKIPDLSSASNLTSLHIYECTNL 744

Query: 379 PSCFITKSLVGLD-MQHSFIKKFGKRLEV-NYSSTGCLLDWRLKHVNLSYSSLLEQIPHF 438
               I +S+  LD ++  ++K+    +++ +Y S   LL      ++LS    LE  P  
Sbjct: 745 R--VIHESVGSLDKLEGLYLKQCTNLVKLPSYLSLKSLLC-----LSLSGCCKLESFPTI 804

Query: 439 PAASNLEELHLS--ECTNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLK 498
             A N++ L     + T ++++  S+  L KL  L L+GC+NL  LP + +++RSLK+L 
Sbjct: 805 --AENMKSLRTLNLDFTAIKELPSSIRYLTKLWTLKLNGCTNLISLPNTIYLLRSLKNLL 864

Query: 499 LSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSH 558
           LS C      PD              C+  +M+  ++ SL     LV    F      SH
Sbjct: 865 LSGCSIFGMFPD-----KWNPTIQPVCSPSKMMETALWSLKVPHFLVPNESF------SH 924

Query: 559 LHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNING 616
           + L  LQ   +S    L+    +A  L  ++L +  F+++   P        LS L +  
Sbjct: 925 ITLLDLQSCNISNANFLDILCDVAPFLSDLRLSENKFSSL---PSCLHKFMSLSNLELRN 962


HSP 2 Score: 1003.4 bits (2593), Expect = 1.9e-289
Identity = 514/681 (75.48%), Postives = 576/681 (84.58%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVGIYGIGGLGKTTLAKALYNKI+SQFEGCCFLSNVRQAS QFNGLVQLQ+NLLYEIL+D
Sbjct: 230 MVGIYGIGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILED 289

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLKFVNLD+GITIIR+RL SKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRN H
Sbjct: 290 DLKFVNLDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSH 349

Query: 121 LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
           LLSSHGFDEMHNI+GL QD+AI+LFSWHAFKESHPSSNYL L ERATSYCKGHPLALVVL
Sbjct: 350 LLSSHGFDEMHNIQGLNQDRAIELFSWHAFKESHPSSNYLDLAERATSYCKGHPLALVVL 409

Query: 181 GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEV-N 240
           GSFLC R QTEW SILD+FENSL+N+IKDILQLSFDGLE  VKDIFLDISCL VGE+  N
Sbjct: 410 GSFLCNRGQTEWRSILDKFENSLNNDIKDILQLSFDGLEGGVKDIFLDISCLFVGEKYNN 469

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRL 300
             K +LSACHLNVDFGI+ILMDLSL+T+E   VQMH LIQQMGH IV+ ES E GKRSRL
Sbjct: 470 CAKKMLSACHLNVDFGIMILMDLSLVTIEKDRVQMHGLIQQMGHSIVHNESFESGKRSRL 529

Query: 301 WLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQA-FRSMKNLRVLIVQNARFSTKI 360
           W  +DIW VFVNNSGTD IKAIKLDL NP  ++VDP+A FRSMKNLR+LI++NA+  TKI
Sbjct: 530 WSERDIWNVFVNNSGTDAIKAIKLDLPNPINVNVDPKAFFRSMKNLRLLIIRNAQVCTKI 589

Query: 361 KHLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWR 420
           K+L +SLKWI+W GFAHR+ PSCFITK+LVGLD++ SFIK+FGKRLE       C    R
Sbjct: 590 KYLPNSLKWIEWQGFAHRTFPSCFITKNLVGLDLRRSFIKRFGKRLE------DC---ER 649

Query: 421 LKHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLK 480
           LKHV+LSYS+LLE+IP   AASNLEEL+L  CTNL  IDKSVFSL+KLT+LN  GCSNLK
Sbjct: 650 LKHVDLSYSTLLEKIPDLSAASNLEELYLINCTNLGMIDKSVFSLNKLTVLNFKGCSNLK 709

Query: 481 KLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLV 540
           KLP  YFM  SLK L LSYC++LE+IPDLS ASNL+SL L  CTNLR+IHES+GSL++LV
Sbjct: 710 KLPKGYFMFSSLKILNLSYCQELEKIPDLSSASNLQSLLLNGCTNLRVIHESVGSLNELV 769

Query: 541 TLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVP 600
            L L +C NL+KLPS+L LKSL YL L GC KLE+FPTIAEN+KS++ LDL  TAIKE+P
Sbjct: 770 LLDLGQCTNLSKLPSYLRLKSLVYLVLFGCGKLESFPTIAENMKSLRCLDLHSTAIKELP 829

Query: 601 PSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 660
            S GYLTQL +L++ GCTN IS PNTI     YLLR+L  + L GCSRFE+ PH+W   I
Sbjct: 830 SSLGYLTQLDKLHLTGCTNLISLPNTI-----YLLRNLNELHLGGCSRFEMFPHKWVPTI 889

Query: 661 QPACSFSKMMETTSWSSEFPH 680
           QP CS SKMME  SWS EFPH
Sbjct: 890 QPVCSPSKMMEAASWSLEFPH 896

BLAST of MELO3C004303T1 vs. NCBI nr
Match: gi|778666722|ref|XP_011648798.1| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 52.4 bits (124), Expect = 3.7e-03
Identity = 54/230 (23.48%), Postives = 98/230 (42.61%), Query Frame = 1

Query: 432  QIPHFPA-ASNLEELHLSEC--TNLRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVR 491
            ++  FP  A N++ L   +   T ++++  S+  L +L  L+L GC+NL  LP + +++R
Sbjct: 792  KLESFPTIAENMKSLRCLDLHSTAIKELPSSLGYLTQLDKLHLTGCTNLISLPNTIYLLR 851

Query: 492  SLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNL 551
            +L  L L  C + E  P       ++ +    C+  +M+  +  SL+    +V       
Sbjct: 852  NLNELHLGGCSRFEMFPH-KWVPTIQPV----CSPSKMMEAASWSLEFPHLVVPNE---- 911

Query: 552  AKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLS 611
              + SH  L  L+   +S  + LE    +A  L  ++L +  F+++   P        LS
Sbjct: 912  -SICSHFTLLDLKSCNISSAKFLEILCDVAPFLSDLRLSENKFSSL---PSCLHKFMSLS 971

Query: 612  RLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQII 659
             L +  C      PN        L +++ N+   GC      P     II
Sbjct: 972  NLELRNCKFLQEIPN--------LPQNIRNLDASGCKSLARSPDNIVDII 1000


HSP 2 Score: 998.4 bits (2580), Expect = 6.0e-288
Identity = 511/707 (72.28%), Postives = 585/707 (82.74%), Query Frame = 1

Query: 1    MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
            MVGIYGIGG+GKTTLAKALYNKI+SQFEGCCFLSNVR+ASKQFNGL QLQE+LLYEIL  
Sbjct: 299  MVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLAQLQESLLYEILMV 358

Query: 61   DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
            DLK VNLDRGI IIR+RL SKKVLIVLDDVDKLEQLEALVGG DWFG+GS+IIVTTRN+H
Sbjct: 359  DLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFGKGSRIIVTTRNKH 418

Query: 121  LLSSHGFDEMHNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVVL 180
            LL SHGFDE+HNI GL +DKAI+LFSWHAFK++ PSSNYL L +RATSYCKGHPLALVVL
Sbjct: 419  LLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRATSYCKGHPLALVVL 478

Query: 181  GSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVNY 240
            GSFLC RDQ EW SILDEFENSL+ +IKDILQLSFDGLED++KDIFLDISCLLVGE+V Y
Sbjct: 479  GSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFLDISCLLVGEKVEY 538

Query: 241  VKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRLW 300
            VK++L ACH+N+DFG+I+LMDLSLIT+EN  VQMHDLI+QMG KIV GES E GKRSRLW
Sbjct: 539  VKDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVCGESLELGKRSRLW 598

Query: 301  LAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIKH 360
            L QD+WEV VNNSGTD IKAIKLD  NPTRL V+ QAFR MKNLR+LIVQNARFSTKI++
Sbjct: 599  LVQDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLLIVQNARFSTKIEY 658

Query: 361  LSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKR--------LEVN-YSS- 420
            L DSLKWIKWHGF   +LPSCFITK+LVGLD+Q+SF+K FGKR          +N Y S 
Sbjct: 659  LPDSLKWIKWHGFPQPTLPSCFITKNLVGLDLQYSFMKTFGKRLEVSFISIYAINGYFSL 718

Query: 421  --------TGC----------LLDWRLKHVNLSYSSLLEQIPHFPAASNLEELHLSECTN 480
                     GC           +   L+++NLS+   LE+IP F AASNLEEL+L  CTN
Sbjct: 719  DKLTILNLAGCSNLKKLPRGYFILRSLRYLNLSHCKKLEKIPDFSAASNLEELYLFNCTN 778

Query: 481  LRKIDKSVFSLDKLTILNLDGCSNLKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASN 540
            LR IDKSVFSL KLTILNLD CSNLKKLPTSY+ + SL++L LSYCKKLE+IPDLS ASN
Sbjct: 779  LRMIDKSVFSLHKLTILNLDVCSNLKKLPTSYYKLWSLQYLNLSYCKKLEKIPDLSAASN 838

Query: 541  LESLYLEDCTNLRMIHESIGSLDKLVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLE 600
            L+SL L +CTNLR+IHES+GSL KL+ + L  C NLAKLP++L LKSL+YLGLS C KLE
Sbjct: 839  LQSLCLHECTNLRLIHESVGSLYKLIDMDLSGCTNLAKLPTYLRLKSLRYLGLSECCKLE 898

Query: 601  NFPTIAENLKSIKLLDLDFTAIKEVPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYL 660
            +FP+IAEN++S++ LD+DFTAIKE+P S GYLTQL RLN+ GCTN IS PNTI     YL
Sbjct: 899  SFPSIAENMESLRELDMDFTAIKELPSSIGYLTQLYRLNLTGCTNLISLPNTI-----YL 958

Query: 661  LRSLENIFLRGCSRFEIVPHEWGQIIQPACSFSKMMETTSWSSEFPH 680
            LR+L+ + L GCSRFE+ PH+W   IQP CS SKMME TSWS E+PH
Sbjct: 959  LRNLDKLLLSGCSRFEMFPHKWDPTIQPVCSPSKMMEATSWSLEYPH 1000

BLAST of MELO3C004303T1 vs. NCBI nr
Match: gi|659071570|ref|XP_008460682.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 59.7 bits (143), Expect = 2.3e-05
Identity = 61/242 (25.21%), Postives = 100/242 (41.32%), Query Frame = 1

Query: 419  LKHVNLSYSSLLEQIPHFPAASNLEELHLSEC--TNLRKIDKSVFSLDKLTILNLDGCSN 478
            L+++ LS    LE  P    A N+E L   +   T ++++  S+  L +L  LNL GC+N
Sbjct: 886  LRYLGLSECCKLESFPSI--AENMESLRELDMDFTAIKELPSSIGYLTQLYRLNLTGCTN 945

Query: 479  LKKLPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDK 538
            L  LP + +++R+L  L LS C + E  P        +      C+  +M+  +  SL+ 
Sbjct: 946  LISLPNTIYLLRNLDKLLLSGCSRFEMFP-----HKWDPTIQPVCSPSKMMEATSWSLE- 1005

Query: 539  LVTLVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKE 598
                          L SH  L  LQ   +S  + LE    +A  L  ++L +  F+++  
Sbjct: 1006 -----YPHLLPNESLCSHFTLLDLQSCNISNAKFLEILCDVAPFLSDLRLSENKFSSL-- 1065

Query: 599  VPPSTGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWGQ 658
             P        L  L +  C      PN        L ++++N+   GC      P     
Sbjct: 1066 -PSCLHKFMSLWNLELKNCKFLQEIPN--------LPQNIQNLDASGCKSLARSPDNIMD 1103


HSP 2 Score: 958.0 bits (2475), Expect = 8.9e-276
Identity = 494/683 (72.33%), Postives = 563/683 (82.43%), Query Frame = 1

Query: 1   MVGIYGIGGLGKTTLAKALYNKISSQFEGCCFLSNVRQASKQFNGLVQLQENLLYEILKD 60
           MVG+YGIGG+GKTTLAKALYNKI++QFEGCCFL +VR+ + + +GLV+LQE LL +ILK+
Sbjct: 254 MVGMYGIGGIGKTTLAKALYNKIANQFEGCCFLQDVRREASK-HGLVKLQETLLNDILKE 313

Query: 61  DLKFVNLDRGITIIRSRLHSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNRH 120
           DLK V+ DRGI IIRSRL SKKVLIVLDDVD  EQLEALVGGRDWFG+GSKIIVTTRN H
Sbjct: 314 DLKVVSRDRGINIIRSRLCSKKVLIVLDDVDDREQLEALVGGRDWFGRGSKIIVTTRNEH 373

Query: 121 LLSSHGFDEM-HNIRGLYQDKAIKLFSWHAFKESHPSSNYLGLVERATSYCKGHPLALVV 180
           LL SHGFD+  H I+ L QD A++LFSWHAFK+SHPSSNYLGL ERAT+YCKG  LALVV
Sbjct: 374 LLFSHGFDDQKHKIQELNQDHALELFSWHAFKKSHPSSNYLGLSERATNYCKGLSLALVV 433

Query: 181 LGSFLCTRDQTEWISILDEFENSLSNNIKDILQLSFDGLEDRVKDIFLDISCLLVGEEVN 240
           LGSFL  RDQ EW  ILDEFE SL  +IKD+LQLSFDGLED+ KDIFLDISCLLVGEE N
Sbjct: 434 LGSFLRGRDQAEWNCILDEFETSLRKDIKDVLQLSFDGLEDKAKDIFLDISCLLVGEEYN 493

Query: 241 YVKNILSACHLNVDFGIIILMDLSLITVENGTVQMHDLIQQMGHKIVYGESPEPGKRSRL 300
             K +LSACHLN+DFGI+IL+DLSL+T+E   VQMH+LIQQMG  IV+ ES EPGKRSRL
Sbjct: 494 CAKKMLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRL 553

Query: 301 WLAQDIWEVFVNNSGTDTIKAIKLDLANPTRLHVDPQAFRSMKNLRVLIVQNARFSTKIK 360
           WL QDIWEVFVNNSGTD +KAIKLDL NPT+L+VDPQAFRSMKNLR+LI++NA+F  KIK
Sbjct: 554 WLVQDIWEVFVNNSGTDAVKAIKLDLPNPTKLNVDPQAFRSMKNLRLLIIRNAQFCRKIK 613

Query: 361 HLSDSLKWIKWHGFAHRSLPSCFITKSLVGLDMQHSFIKKFGKRLEVNYSSTGCLLDWRL 420
           +L +SLKWI+W GFAHRSLPSCFITK+LVGLD++HS IK+FGKRLE      GC    RL
Sbjct: 614 YLPNSLKWIEWRGFAHRSLPSCFITKNLVGLDLRHSSIKRFGKRLE------GC---ERL 673

Query: 421 KHVNLSYSSLLEQIPHFPAASNLEELHLSECTNLRKIDKSVFSLDKLTILNLDGCSNLKK 480
           KHV+LSYS+LLEQI  F  ASNLEELHL  CTNL  IDKSVFSL KL++LNLDGC NL+K
Sbjct: 674 KHVDLSYSTLLEQINDFSPASNLEELHLINCTNLGMIDKSVFSLYKLSVLNLDGCCNLQK 733

Query: 481 LPTSYFMVRSLKHLKLSYCKKLERIPDLSLASNLESLYLEDCTNLRMIHESIGSLDKLVT 540
           LP  YFM+ SLK L L YCKKLE+IPDLS ASNL+ LYL++CTNLR+IHES+GSLDKL  
Sbjct: 734 LPRGYFMLSSLKELNLCYCKKLEKIPDLSAASNLKRLYLQECTNLRVIHESVGSLDKLNH 793

Query: 541 LVLRRCFNLAKLPSHLHLKSLQYLGLSGCRKLENFPTIAENLKSIKLLDLDFTAIKEVPP 600
           L LR+C  L KLPS+L LKSL  L LSGC KLE+FPTIAEN+KS++ LD+DFTAIKE+P 
Sbjct: 794 LDLRQCTKLVKLPSYLRLKSLSNLLLSGCCKLESFPTIAENMKSLRELDMDFTAIKELPS 853

Query: 601 STGYLTQLSRLNINGCTNPISHPNTICLSRLYLLRSLENIFLRGCSRFEIVPHEWG---Q 660
           S GYLT LS L +NGCTN IS PNTI     YLLR+LEN+ L GCS F + PH W     
Sbjct: 854 SIGYLTNLSILKLNGCTNLISLPNTI-----YLLRNLENLLLSGCSIFGMFPHTWDPTIP 913

Query: 661 IIQPACSFSKMMETTSWSSEFPH 680
            IQ  CS SKMMET SWS EFPH
Sbjct: 914 TIQQVCSPSKMMETASWSLEFPH 921

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TMVRN_NICGU1.8e-9837.02TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1[more]
TAO1_ARATH5.9e-8631.71Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1[more]
WRK19_ARATH1.6e-8333.96Probable WRKY transcription factor 19 OS=Arabidopsis thaliana GN=WRKY19 PE=2 SV=... [more]
RPP1_ARATH2.4e-7931.09Probable disease resistance protein RPP1 OS=Arabidopsis thaliana GN=RPP1 PE=2 SV... [more]
Y4117_ARATH3.5e-7832.65Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g111... [more]
Match NameE-valueIdentityDescription
A0A0A0LLL0_CUCSA1.3e-28975.48Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1[more]
A0A0A0LLL0_CUCSA2.6e-0323.48Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1[more]
A0A0A0LJM0_CUCSA1.9e-0625.25Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022270 PE=4 SV=1[more]
E5GB33_CUCME3.1e-9468.20TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LLK3_CUCSA6.0e-7760.15Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020890 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36930.23.1e-10137.19 Disease resistance protein (TIR-NBS-LRR class) family[more]
AT5G17680.16.9e-10135.77 disease resistance protein (TIR-NBS-LRR class), putative[more]
AT4G12010.12.0e-9233.87 Disease resistance protein (TIR-NBS-LRR class) family[more]
AT5G46450.11.6e-8931.53 Disease resistance protein (TIR-NBS-LRR class) family[more]
AT5G18360.17.9e-8933.73 Disease resistance protein (TIR-NBS-LRR class) family[more]
Match NameE-valueIdentityDescription
gi|659071574|ref|XP_008460703.1|3.0e-29575.85PREDICTED: TMV resistance protein N-like [Cucumis melo][more]
gi|659071574|ref|XP_008460703.1|2.2e-0324.79PREDICTED: TMV resistance protein N-like [Cucumis melo][more]
gi|778666836|ref|XP_011648823.1|2.7e-0625.25PREDICTED: LOW QUALITY PROTEIN: TMV resistance protein N-like [Cucumis sativus][more]
gi|778666722|ref|XP_011648798.1|3.7e-0323.48PREDICTED: TMV resistance protein N-like [Cucumis sativus][more]
gi|659071570|ref|XP_008460682.1|2.3e-0525.21PREDICTED: TMV resistance protein N-like [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002182NB-ARC
IPR011991Winged helix-turn-helix DNA-binding domain
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0043531ADP binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007165 signal transduction
cellular_component GO:0005575 cellular_component
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C004303MELO3C004303gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C004303T1MELO3C004303T1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C004303T1.cds3MELO3C004303T1.cds3CDS
MELO3C004303T1.cds2MELO3C004303T1.cds2CDS
MELO3C004303T1.cds1MELO3C004303T1.cds1CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 1..240
score: 5.8
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 206..308
score: 2.15
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 2..145
score: 4.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 2..229
score: 1.81
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 1..16
score: 7.0E-24coord: 555..571
score: 7.0E-24coord: 77..91
score: 7.0E-24coord: 170..184
score: 7.0
NoneNo IPR availablePANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 1..596
score: 1.0E
NoneNo IPR availablePANTHERPTHR11017:SF162SUBFAMILY NOT NAMEDcoord: 1..596
score: 1.0E