MELO3C003429T2 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C003429T2
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionE3 ubiquitin-protein ligase
Locationchr4 : 1124244 .. 1127342 (+)
Sequence length1971
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAGTCCGGAAGAGGAGGCCGGTGGACTGATCACAATCGAAACCAAGAGGTTTGGGTTGCCAAACACACGAATCATTCACACAATCACGCTTCTTCTTCCGCCTCACAACCCTCCCACCTCTCTAATCCTCCTCCTACCGGTCGTAATTTCAGTTACGAGCATCGCCATCCCCGCCGCCCCCGTCCCCAACCTGTCCACAAACCGGAGGTCACTTCTCTGGCCGATGGAATAGAATCTTTCACTTTCGACGAAAAGCCTGTGGACTTCGATGTTGCGAAACCTAACTGTGAGGTTAAACAACAGACACCTGGAAATTCTTCCTCAGCTGAGGCTGCGGATGATGTTTATAGTAGGTTAGAGATGCTGCAGCGGAGTTCCGAGGAGCCGGTGTTGTCGGAGGAGCAGCTCAGTATTAATGATCAGTTGCAGGAAGATGAGGTATCGAATTGTCAATGATATGAAAGCGGAATTGACTGTTGTCACTTTCAGTTGAAGTTTGTTCATATACGTTATGTGTTGCAATTTGCTACCAATTCATTTTTGTTCTAAATATTTGTTTTTCATGTCCAATTTTGAACTGTTTCTCATCATGTTATGGAAGTAATGGCGTCTTATTTACTGTTTTATTGGCAAATCTTCTATAGTTGCTGGCTTTGGAGTCCATATATGGAGAAAATGTCTATATCCTCGATGAATATAAAGGGCAGCGCCGTTTCCAGGTTCACAACTATCCAATAAATTTATAGTTATTGTTACGTACACAATTCTTTGGAATTTGGACGTCAAACTATGCTATTATTCTGCAGATTTTCATACACATTGAGAATCCTGGCGATATTACTATAACTGCAAAGCTTAATTCATCTAGTTCCCTTGAGATCAAAAGTCCTAATTCAGATGAATTCTCCTGTTCTTTCAATGTCAAGTATCTTAACCCCATTGTTTTGACTTGTTTATTACCAAAATCGTATCCTAGTCATCTTCCTCCATATTATACAATCTCTATTCGGTGGTTGGACTCTGCTAGAATTTCTAGGCTTTGCTCTGTGTTGGATGGGATATGGAGTGAACAAGTGGGCCAGGAAGTTGTATATCAATGGGTAGAATGGCTACAAATGTCATCTCTGGCTTATCTTCAGTTAGATAAAGAGATTATGCTTGGTCCGTACGATAAGGGACATTCAGGAGATACTCGGGCAGTTTCAGGAAGTGTTTCTCCAGAAGTTGATATTCCTGCTATTATAAATTATGACAATCAGAGACGTGATGAGGAATTCTGCATGAATTTGCAAGAGTGCTGCATCTGTTTAAGTCAGTTTGCAGGTAAGCTCCATCACTGGCTCAATATAAGAATAAGATGCTATTGTTCTCCAACTCCTACTTCTATATGTATGCATCAACGAGAATTGTCTTAATAACAATCATTTGTAACATATTAATTCCGTCCAAGCTTCTAATTTCAGATTGCATAAAAGAATAACACGAAAGATTTTGGGAAATGGTGTTAGTTACTAGCTTTGGGTTTGCCTTTCTCCCTCTGAGGCTGGACTCTGGAGAGATTTGCAATTGTAAAGAAGAGAAATTATCTGTTATACAATTTGACTGACTACCCCACTTTAAGAAGAGGTTCTATTGTTAGTCAAAACTGCAATATCTGCCTCCTTAGGAATTGAAAATGCCTTGGCCTATTTATTAATGGAATCTGATCAAAATCATGCAGATGGGCGTCAATTTTCTTTACATAATCACTTTGTGACTGCATTAACAAAAAAGGTTGTATTTGTTTCGGGGTGAATTTCCATGAGTGATGTTTTGATATTAGAGAAATAAATGGCAATGCCATCAATTTTACTCTTTCAGACTCTATGTAGAACTCTTACCATTAGATGTGAACTTTTAACTCCAGCTTCTTCATTTGTTGGTTGGACATAATCAGGTACGAAGTTTGTTAAATTGCCATGTAAACATTACTTTTGCTGGAAGTGCATGGAGACATACTCGAGCATGCATGTCAAGGAAGGCACAGTAAGCAAACTCAATTGCCCAGATGCCAAATGTGATGTTATGGTTCCTCCTGGTCTTCTGAAGCAATTGCTTGGCGATGAGGAGTTTGAAAGATGGGAATCCATGATGCTCACGAAAACACTGGAATCGATGTCAGATGTTGTCTATTGCCCAAGGTGTGAAACACCCTGCCTAGAGGATGTCGATCATGATGCCCAATGCTCTAAGTGTTACTTCAGTTTTTGTACACTCTGTAGCGAGCGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGTTAGCTAAGTACCAAGTCCACTCAATCCATGTGCAATTTTTCTGTTTTGTGGGAGATATTCATTAGCACTTATCCTTAGAAGTGATAAACCTTTTCCATTCCTTAGCCTCATTAATTTATTATTCTTAAATCTTTTTCAGGAGCGTCAAAATTCATCTCAGTTAGGCAGCGAACAAAGAAGAAAGGAGCGTGAGATGATAAATGAGCTTATTAGTGTCAAGGAAATACTTCGTGATGCCAAGCAATGCCCATCTTGTAAAATGGCAATATCACGGACCGAGGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGTAGCAAGGCAATTGATGGATATGACCATTTCAGGTTTGTTAGTGTGAGCGAGTATTAGTTTAGTGCTGATTTCTTTCAAAGATAAATAAATTGACTCGCTCATCTTAAATGAAGGGAGGGATCATGTGAATTGTTCCCGCAAGAAGCCATTCAACAGTGGGAAGAGCGCATGAATGCACGGCAGGTATTGGGCCAGATTCAAGCTGAACTTTTTCCTGAAAATGGTCGTCCATGTCCTAATTGTGGTCAACTTAATGCCAAGGTGAGCAGAAGTAGGACGCATTTACTAATGGATATGGTGTATGTTTAGGTAGTTTCGTGTGTTACTGCAAAACCGACTGAACTATACAAAGAAAATAACGCATTCTGAACGGAAAACTGTTCATAATATAAAAGTTTTGCCCTATCATCGCCCATCTAGTCAATCTAGGATTTC

mRNA sequence

ATGAGAAAGTCCGGAAGAGGAGGCCGGTGGACTGATCACAATCGAAACCAAGAGGTTTGGGTTGCCAAACACACGAATCATTCACACAATCACGCTTCTTCTTCCGCCTCACAACCCTCCCACCTCTCTAATCCTCCTCCTACCGGTCGTAATTTCAGTTACGAGCATCGCCATCCCCGCCGCCCCCGTCCCCAACCTGTCCACAAACCGGAGGTCACTTCTCTGGCCGATGGAATAGAATCTTTCACTTTCGACGAAAAGCCTGTGGACTTCGATGTTGCGAAACCTAACTGTGAGGTTAAACAACAGACACCTGGAAATTCTTCCTCAGCTGAGGCTGCGGATGATGTTTATAGTAGGTTAGAGATGCTGCAGCGGAGTTCCGAGGAGCCGGTGTTGTCGGAGGAGCAGCTCAGTATTAATGATCAGTTGCAGGAAGATGAGTTGCTGGCTTTGGAGTCCATATATGGAGAAAATGTCTATATCCTCGATGAATATAAAGGGCAGCGCCGTTTCCAGATTTTCATACACATTGAGAATCCTGGCGATATTACTATAACTGCAAAGCTTAATTCATCTAGTTCCCTTGAGATCAAAAGTCCTAATTCAGATGAATTCTCCTGTTCTTTCAATGTCAAGTATCTTAACCCCATTGTTTTGACTTGTTTATTACCAAAATCGTATCCTAGTCATCTTCCTCCATATTATACAATCTCTATTCGGTGGTTGGACTCTGCTAGAATTTCTAGGCTTTGCTCTGTGTTGGATGGGATATGGAGTGAACAAGTGGGCCAGGAAGTTGTATATCAATGGGTAGAATGGCTACAAATGTCATCTCTGGCTTATCTTCAGTTAGATAAAGAGATTATGCTTGGTCCGTACGATAAGGGACATTCAGGAGATACTCGGGCAGTTTCAGGAAGTGTTTCTCCAGAAGTTGATATTCCTGCTATTATAAATTATGACAATCAGAGACGTGATGAGGAATTCTGCATGAATTTGCAAGAGTGCTGCATCTGTTTAAGTCAGTTTGCAGGTACGAAGTTTGTTAAATTGCCATGTAAACATTACTTTTGCTGGAAGTGCATGGAGACATACTCGAGCATGCATGTCAAGGAAGGCACAGTAAGCAAACTCAATTGCCCAGATGCCAAATGTGATGTTATGGTTCCTCCTGGTCTTCTGAAGCAATTGCTTGGCGATGAGGAGTTTGAAAGATGGGAATCCATGATGCTCACGAAAACACTGGAATCGATGTCAGATGTTGTCTATTGCCCAAGGTGTGAAACACCCTGCCTAGAGGATGTCGATCATGATGCCCAATGCTCTAAGTGTTACTTCAGTTTTTGTACACTCTGTAGCGAGCGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGAGCGTCAAAATTCATCTCAGTTAGGCAGCGAACAAAGAAGAAAGGAGCGTGAGATGATAAATGAGCTTATTAGTGTCAAGGAAATACTTCGTGATGCCAAGCAATGCCCATCTTGTAAAATGGCAATATCACGGACCGAGGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGTAGCAAGGCAATTGATGGATATGACCATTTCAGGGAGGGATCATGTGAATTGTTCCCGCAAGAAGCCATTCAACAGTGGGAAGAGCGCATGAATGCACGGCAGGTATTGGGCCAGATTCAAGCTGAACTTTTTCCTGAAAATGGTCGTCCATGTCCTAATTGTGGTCAACTTAATGCCAAGGTGAGCAGAAGTAGGACGCATTTACTAATGGATATGGTGTATGTTTAGGTAGTTTCGTGTGTTACTGCAAAACCGACTGAACTATACAAAGAAAATAACGCATTCTGAACGGAAAACTGTTCATAATATAAAAGTTTTGCCCTATCATCGCCCATCTAGTCAATCTAGGATTTC

Coding sequence (CDS)

ATGAGAAAGTCCGGAAGAGGAGGCCGGTGGACTGATCACAATCGAAACCAAGAGGTTTGGGTTGCCAAACACACGAATCATTCACACAATCACGCTTCTTCTTCCGCCTCACAACCCTCCCACCTCTCTAATCCTCCTCCTACCGGTCGTAATTTCAGTTACGAGCATCGCCATCCCCGCCGCCCCCGTCCCCAACCTGTCCACAAACCGGAGGTCACTTCTCTGGCCGATGGAATAGAATCTTTCACTTTCGACGAAAAGCCTGTGGACTTCGATGTTGCGAAACCTAACTGTGAGGTTAAACAACAGACACCTGGAAATTCTTCCTCAGCTGAGGCTGCGGATGATGTTTATAGTAGGTTAGAGATGCTGCAGCGGAGTTCCGAGGAGCCGGTGTTGTCGGAGGAGCAGCTCAGTATTAATGATCAGTTGCAGGAAGATGAGTTGCTGGCTTTGGAGTCCATATATGGAGAAAATGTCTATATCCTCGATGAATATAAAGGGCAGCGCCGTTTCCAGATTTTCATACACATTGAGAATCCTGGCGATATTACTATAACTGCAAAGCTTAATTCATCTAGTTCCCTTGAGATCAAAAGTCCTAATTCAGATGAATTCTCCTGTTCTTTCAATGTCAAGTATCTTAACCCCATTGTTTTGACTTGTTTATTACCAAAATCGTATCCTAGTCATCTTCCTCCATATTATACAATCTCTATTCGGTGGTTGGACTCTGCTAGAATTTCTAGGCTTTGCTCTGTGTTGGATGGGATATGGAGTGAACAAGTGGGCCAGGAAGTTGTATATCAATGGGTAGAATGGCTACAAATGTCATCTCTGGCTTATCTTCAGTTAGATAAAGAGATTATGCTTGGTCCGTACGATAAGGGACATTCAGGAGATACTCGGGCAGTTTCAGGAAGTGTTTCTCCAGAAGTTGATATTCCTGCTATTATAAATTATGACAATCAGAGACGTGATGAGGAATTCTGCATGAATTTGCAAGAGTGCTGCATCTGTTTAAGTCAGTTTGCAGGTACGAAGTTTGTTAAATTGCCATGTAAACATTACTTTTGCTGGAAGTGCATGGAGACATACTCGAGCATGCATGTCAAGGAAGGCACAGTAAGCAAACTCAATTGCCCAGATGCCAAATGTGATGTTATGGTTCCTCCTGGTCTTCTGAAGCAATTGCTTGGCGATGAGGAGTTTGAAAGATGGGAATCCATGATGCTCACGAAAACACTGGAATCGATGTCAGATGTTGTCTATTGCCCAAGGTGTGAAACACCCTGCCTAGAGGATGTCGATCATGATGCCCAATGCTCTAAGTGTTACTTCAGTTTTTGTACACTCTGTAGCGAGCGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGAGCGTCAAAATTCATCTCAGTTAGGCAGCGAACAAAGAAGAAAGGAGCGTGAGATGATAAATGAGCTTATTAGTGTCAAGGAAATACTTCGTGATGCCAAGCAATGCCCATCTTGTAAAATGGCAATATCACGGACCGAGGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGTAGCAAGGCAATTGATGGATATGACCATTTCAGGGAGGGATCATGTGAATTGTTCCCGCAAGAAGCCATTCAACAGTGGGAAGAGCGCATGAATGCACGGCAGGTATTGGGCCAGATTCAAGCTGAACTTTTTCCTGAAAATGGTCGTCCATGTCCTAATTGTGGTCAACTTAATGCCAAGGTGAGCAGAAGTAGGACGCATTTACTAATGGATATGGTGTATGTTTAG

Protein sequence

MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPRRPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKVSRSRTHLLMDMVYV*
BLAST of MELO3C003429T2 vs. Swiss-Prot
Match: RNF14_MOUSE (E3 ubiquitin-protein ligase RNF14 OS=Mus musculus GN=Rnf14 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 2.3e-57
Identity = 146/461 (31.67%), Postives = 229/461 (49.67%), Query Frame = 1

Query: 143 QLQEDELLALESIYGENVYILDEYKGQRRFQ---IFIHIENPGDITITAKLNSSSSLEIK 202
           + QEDELLAL SIY       DE++     Q     I+++ P +  I    NS+ SL+  
Sbjct: 7   EAQEDELLALASIYDA-----DEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNESLQ-- 66

Query: 203 SPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIW 262
             NS      + + +L P+VL   LP  YPS  PP +T+S +WL   ++S LC  LD +W
Sbjct: 67  --NS---GFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLW 126

Query: 263 SEQVGQEVVYQWVEWLQMSSLAYLQLDK--EIMLGPYDK--------GHSGDTRAVSGSV 322
            E  G+ V++ W+++L+  +L YL +    E+ +G   K          S     V G+ 
Sbjct: 127 EEHRGRVVLFAWMQFLKEETLTYLNIVSPFELKMGSQKKVQRRATAQASSSTELGVGGAA 186

Query: 323 SPEVD-------------------IPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFV 382
           + +VD                   I  I++++  R+ + F   L  C IC  +  G+  +
Sbjct: 187 AADVDQEETVDERAVQDVESLSSLIQEILDFNQARQTKCFNSKLFLCSICFCEKLGSDCM 246

Query: 383 K-LPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWES 442
             L CKH +C  C++ Y  + +K+G V  LNCP+ +C  +  PG +K+L+  + F R++ 
Sbjct: 247 YFLECKHVYCKACLKDYFEIQIKDGQVKCLNCPEPQCPSVATPGQVKELVEADLFARYDR 306

Query: 443 MMLTKTLESMSDVVYCPR--CETPCLEDVDHD-AQCSKCYFSFCTLCSERRHVGIEC-MT 502
           ++L  TL+ M+DVVYCPR  C+ P +++     A CS C F+FCTLC    H    C +T
Sbjct: 307 LLLQSTLDLMADVVYCPRPCCQLPVMQEPGGTMAICSSCNFAFCTLCRLTYHGLSPCKVT 366

Query: 503 PEMKLRLLEERQNSSQLGS---EQRRKER---EMINELISVKEILRDAKQCPSCKMAISR 558
            E  + L  E   + +      EQR  +R   + + E+ S   + +++K CP C   I +
Sbjct: 367 AEKLIDLRNEYLQADEATKRFLEQRYGKRVIQKALEEMESKDWLEKNSKSCPCCGTPIQK 426

BLAST of MELO3C003429T2 vs. Swiss-Prot
Match: RNF14_HUMAN (E3 ubiquitin-protein ligase RNF14 OS=Homo sapiens GN=RNF14 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.4e-56
Identity = 145/460 (31.52%), Postives = 229/460 (49.78%), Query Frame = 1

Query: 143 QLQEDELLALESIYGENVYILDEYKGQRRFQ---IFIHIENPGDITITAKLNSSSSLEIK 202
           + QEDELLAL SIY       DE++     Q     I+++ P +  I    NS+  L+  
Sbjct: 7   EAQEDELLALASIYDG-----DEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNECLQ-- 66

Query: 203 SPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIW 262
             NS      + + +L P+VL   LP  YPS  PP +T+S +WL   ++S LC  LD +W
Sbjct: 67  --NS---GFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLW 126

Query: 263 SEQVGQEVVYQWVEWLQMSSLAYLQLDK--EIMLGPYDK-----------------GHSG 322
            E  G  V++ W+++L+  +LAYL +    E+ +G   K                 G +G
Sbjct: 127 EEHRGSVVLFAWMQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAG 186

Query: 323 ---------DTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVK 382
                    D RAV    S    I  I+++D  ++ + F   L  C IC  +  G++ + 
Sbjct: 187 SDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLFLCSICFCEKLGSECMY 246

Query: 383 -LPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESM 442
            L C+H +C  C++ Y  + +++G V  LNCP+ KC  +  PG +K+L+  E F R++ +
Sbjct: 247 FLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRL 306

Query: 443 MLTKTLESMSDVVYCPR--CETPCLEDVDHD-AQCSKCYFSFCTLCSERRHVGIEC-MTP 502
           +L  +L+ M+DVVYCPR  C+ P +++       CS C F+FCTLC    H    C +T 
Sbjct: 307 LLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPCKVTA 366

Query: 503 EMKLRLLEERQNSSQLGS---EQRRKER---EMINELISVKEILRDAKQCPSCKMAISRT 558
           E  + L  E   + +      +QR  +R   + + E+ S + + +++K CP C   I + 
Sbjct: 367 EKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKL 426

BLAST of MELO3C003429T2 vs. Swiss-Prot
Match: ITT1_SCHPO (E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=itt1 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 3.6e-34
Identity = 118/447 (26.40%), Postives = 199/447 (44.52%), Query Frame = 1

Query: 132 VLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIE--NPGDITITAK 191
           +L  E  S N  L  DEL+AL+SIY E                 IH++  N G + I   
Sbjct: 1   MLEVEVESDNKHLVADELIALQSIYPE-----------------IHLDGNNYGRLNIPVN 60

Query: 192 LNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARIS 251
             S   L  KSP+    + +  V++   +V+   LP++YP + PP + +   WL   +  
Sbjct: 61  TESDYFLSFKSPDESTLTDTIVVRHFPDLVMEFFLPEAYPFNSPPTFFLKSSWLPLKQKR 120

Query: 252 RLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIML-GPYDKGHSGDTRAVSGS 311
            L S L  +W+E +   V++  +E ++  +     L  E++  G +D     D +     
Sbjct: 121 VLTSSLIKLWNE-IHDCVLFDAIEHVRSIATIAFHLPTEMVFPGGFD-----DLKK---- 180

Query: 312 VSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLP-CKHYFCWKCMETYS 371
                    I+ +D   +  EF +   +C +C  +F GT   +L  C H  C  C+  Y 
Sbjct: 181 --------EILAFDKNAKLLEFQIRKFQCNVCFDEFNGTDCFQLTRCGHVSCQSCLRDYY 240

Query: 372 SMHVKEGTVSKLNCPDAKCDVMVPPGLLKQL---LGDEEFERWESMMLTKTLESMSDVVY 431
           +M ++EG  S++ C D  C    P   LK+L   +G +   R++ +   +  E+ S++++
Sbjct: 241 TMCIQEGMFSQIKCIDLDCGKDAPVLTLKELESIVGVQLTNRYKELEEKRRYENDSNIIF 300

Query: 432 CPR--CETPCLEDVDHD-AQCSKCYFSFCTLCSERRHVGIE-CMTPEMKLRLLE------ 491
           CPR  C+ P   D     A C KC F+FC+ C    H  +  C       +L+E      
Sbjct: 301 CPRSFCQGPSKRDPGQKLAICQKCDFAFCSFCQATWHGDLSPCKLEGDSKKLVEMYLNYQ 360

Query: 492 ERQNSSQLGSEQRRKER-------EMINELISVKEILRDAKQCPSCKMAISRTEGCNKMV 551
           E +    L  E+R  +R       ++ N+  + K +L + ++CP+C   + R +GC  M 
Sbjct: 361 ENEPEKALELEKRYGKRIIDRLVEQVKNDEEAEKWVLLNGQRCPTCDRVVERIDGCCHMN 411

BLAST of MELO3C003429T2 vs. Swiss-Prot
Match: ITT1_YEAST (Translation termination inhibitor protein ITT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ITT1 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.8e-26
Identity = 77/267 (28.84%), Postives = 122/267 (45.69%), Query Frame = 1

Query: 337 CCICLSQFAGTKFVKLPCK-----HYFCWKCMETYSSMHVKEGTVSKLNCPD-------- 396
           CCIC+    G + +KLPC+     HY C  C ++Y +  ++E  +S + CP         
Sbjct: 180 CCICMEMEKGVRMIKLPCENANVEHYLCRGCAKSYFTAMIQENRISSVRCPQCEYKELKL 239

Query: 397 -----------AKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMSDV-----VYCPR 456
                      A    ++P   LK+++  E  ER+E M   +    +S       V C R
Sbjct: 240 EDFKSYKKMLKALFTPLIPVSFLKEVIDTELCERYEKMFYNQAATRLSKYCPYACVTCRR 299

Query: 457 CETPCLEDVDHDA--QCSKCYFSFCTLCSERRH-VGIEC-MTPEMKLRLLEERQNSSQLG 516
           C++ C ++   DA  QC KC+F FC  C    H    +C     +   ++EE  + +   
Sbjct: 300 CDSWCTKEDLDDAMIQCQKCHFVFCFDCLHAWHGYNNKCGKKVSLSTDIIEEYLDDTVTS 359

Query: 517 SEQRRK----------EREMINEL------ISVKEILRDAKQCPSCKMAISRTEGCNKMV 552
            E++RK          E E+ + L      +++K+   + ++CP CK+ + R+EGCNKM 
Sbjct: 360 YERKRKLEAKYGRRVLELEVNDYLAEKMLDLAIKKEGSNLQRCPKCKVVVERSEGCNKMK 419

BLAST of MELO3C003429T2 vs. Swiss-Prot
Match: Y5521_DICDI (Uncharacterized protein DDB_G0292642 OS=Dictyostelium discoideum GN=DDB_G0292642 PE=3 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 2.5e-19
Identity = 59/209 (28.23%), Postives = 98/209 (46.89%), Query Frame = 1

Query: 336 ECCICLSQFAGTKFV-KLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGL 395
           EC IC  ++  +  V  L C H +C+ C+  +  + + EG V  ++CP  +C   +    
Sbjct: 615 ECKICYMEYDQSNEVFTLECDHVYCFDCITEHLRILITEGRVLDISCPHPQCKKEIKESE 674

Query: 396 LKQLLGDEEFERWESMMLTKTLESMSDVVYCPR--CETPCLEDVDHDA--QCSKCYFSFC 455
           +  L  ++ + +++   +  +L++   + +CP   C+TP     + +    C KC   FC
Sbjct: 675 IYMLTNEKNWLKYQKFSMIASLKT-EPIKWCPTPDCDTPVRGGSERNPILNCPKCSNDFC 734

Query: 456 TLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQC 515
            +C E  H G +C T  M+L   + R+N S   +         I+ L S K  +   K C
Sbjct: 735 WICGEYSHEGAKCGTEAMEL---QGRKNKSIESAATA-----YIDFLESNKHFV---KPC 794

Query: 516 PSCKMAISRTEGCNKMVCNNCGQYFCYRC 540
           P+CK  I + +GCN M C NC   FC+ C
Sbjct: 795 PTCKSHIEKHDGCNHMTCINCQHQFCWLC 811

BLAST of MELO3C003429T2 vs. TrEMBL
Match: A0A068UXB6_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037831001 PE=4 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.2e-212
Identity = 372/639 (58.22%), Postives = 458/639 (71.67%), Query Frame = 1

Query: 2   RKSGRGGRWTDHNRNQEVWVAKHTNHSHN--------HASSSAS---QPSHLSNPPPTGR 61
           R+ G+   +  H +N E W  K     HN         A S++    + +H   P  T +
Sbjct: 9   RRGGKNSHFYQH-QNDENWTLKRVQ-DHNLPPQFDQQFAPSNSELNVEHNHFEQPTTTSK 68

Query: 62  NFSYE-----------HRHPRRPRPQPVHKPEVTSLADGIESFTFDEKPVDF-------- 121
           N  Y            +R P+  +PQ V K EV+SL  GI+    DEK  DF        
Sbjct: 69  NQKYRQNRRNPGWGLRNRRPQAVKPQFVKKSEVSSL--GIDERN-DEKREDFRDGLGEEL 128

Query: 122 -------DVAKPNCEVKQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQL 181
                  +V +   EV+++  G+  + E  D V  RLE L  S++E  LSEEQL INDQ 
Sbjct: 129 EKIASKSEVVEGKDEVEEKIEGSGLNEEDVDVVQKRLEELLLSAQEAELSEEQLRINDQA 188

Query: 182 QEDELLALESIYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSD 241
           QEDELLA+ESIYG+N++ LD+  G R FQI IHIE P +I++  KLNSS+ +   + +S 
Sbjct: 189 QEDELLAMESIYGDNIFFLDKQNGLRSFQIHIHIETPSEISVAVKLNSSA-VSKANDDSS 248

Query: 242 EFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVG 301
           EFS SF V+YL PI+LTC+LPKSYPSHLPP++TIS +WL S +IS LC+VLD IW+EQ G
Sbjct: 249 EFSYSFKVEYLPPIILTCILPKSYPSHLPPHFTISAQWLSSTKISDLCNVLDSIWNEQSG 308

Query: 302 QEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQ 361
           QEV+YQWVE +  S L++L  D+EI+LGPY      D RA+SGSVSPEVDIP + +Y ++
Sbjct: 309 QEVIYQWVESIHSSCLSFLGFDQEIILGPYAVKQERDRRAISGSVSPEVDIPYMKSYSDE 368

Query: 362 RRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDA 421
           +R E F  N+ ECCIC S+FAGT+FV+LPC+H+FCWKCM+TYS +HVKEGTVSKL C DA
Sbjct: 369 QRHENFSKNIHECCICFSEFAGTEFVRLPCQHFFCWKCMKTYSDIHVKEGTVSKLQCADA 428

Query: 422 KCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSK 481
           KC  M+PPGLL++LLGDEEFERWESM L KTLESM+DV YCPRCET C+ED +  AQCSK
Sbjct: 429 KCGGMIPPGLLRRLLGDEEFERWESMTLQKTLESMTDVTYCPRCETICIEDEEQHAQCSK 488

Query: 482 CYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEIL 541
           CYFSFCTLC E+RHVG+ CMTPEMKL +L+ERQNSSQL  +QRR+E+E+IN+L+SVKEI 
Sbjct: 489 CYFSFCTLCREKRHVGVACMTPEMKLLVLQERQNSSQLKDDQRRREKEIINDLLSVKEIN 548

Query: 542 RDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQ 601
             AKQCPSCKMAISRTEGCNKMVC NCG YFCYRC+K IDGYDHFR+GSCELFP E IQ 
Sbjct: 549 ISAKQCPSCKMAISRTEGCNKMVCGNCGHYFCYRCNKEIDGYDHFRDGSCELFPPETIQH 608

Query: 602 WEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKVSRS 604
           WEERMNARQV+GQIQAE+FPE G  CPNCGQ+N K   +
Sbjct: 609 WEERMNARQVVGQIQAEMFPERGHSCPNCGQINLKAGNN 641

BLAST of MELO3C003429T2 vs. TrEMBL
Match: D7TTU0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0012g01750 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.8e-211
Identity = 368/624 (58.97%), Postives = 461/624 (73.88%), Query Frame = 1

Query: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHN------HASSSASQPSHLSNPPPTGR---N 60
           MR+  RG +     +  + W  K + H+ +      H +  + QP+H S+  P  +   N
Sbjct: 1   MRRGRRGRKLGRPVQADDNWRLKSSLHNQSQPDTTSHENLQSPQPTHDSDFHPKSQSRPN 60

Query: 61  FSYEHRHPRR--PRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSS 120
             +  R+ R    +   V K EV S ++  E    +++    +  +      +QT  N  
Sbjct: 61  SKWVSRNQRAHVAKTTFVKKSEVGSGSEVNEQKQEEDEQKQGEEEEGEGPRNEQTDLNEE 120

Query: 121 SAEAAD------DVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYIL 180
             EA+D      DV SRLE L    EEP LSE++L IN QLQEDELLA++SIYG+NV+IL
Sbjct: 121 VVEASDFTHDVDDVGSRLEKLVVGVEEPELSEDRLRINAQLQEDELLAMQSIYGDNVFIL 180

Query: 181 DEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCL 240
           D  +G + FQI +HIE  G++T+TAKLNSSS    +S  SD+FS SFNV+YL PIVLTCL
Sbjct: 181 DRQQGLQSFQIHVHIEALGELTVTAKLNSSSGRTTESDGSDDFSYSFNVQYLPPIVLTCL 240

Query: 241 LPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYL 300
           LPK+YPSHLPPY+TIS++WLDS  IS+LCS+LD +W EQ GQEV+YQWVEWL  S L+YL
Sbjct: 241 LPKAYPSHLPPYFTISVQWLDSISISKLCSMLDSLWKEQPGQEVLYQWVEWLHSSCLSYL 300

Query: 301 QLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQ 360
             DKEI+LGPY+ G+S D RA+SGSVS +VDIP++ +Y++++R E F  N  ECCIC ++
Sbjct: 301 GFDKEIVLGPYNMGNSEDRRAISGSVSLDVDIPSMKSYNDEKRHENFSKNFHECCICFTE 360

Query: 361 FAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEE 420
           +AGT+F+KLPC+H+FCWKCM+TYS MHVKEGT S+L CP+ KC  MVPP LLK+LLGDEE
Sbjct: 361 YAGTEFIKLPCQHFFCWKCMKTYSEMHVKEGTGSQLLCPETKCRAMVPPVLLKRLLGDEE 420

Query: 421 FERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIEC 480
           FE WES+ML KTLESMSD+ YCPRCET C+ED D  AQCSKC+FSFCTLC +RRHVG+EC
Sbjct: 421 FEHWESLMLQKTLESMSDMTYCPRCETICIEDEDQHAQCSKCFFSFCTLCRDRRHVGLEC 480

Query: 481 MTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGC 540
           MTPE+KL +L+ RQ+SSQL  EQR+KEREMINEL+SVKEILRDAKQCPSCKMAISRTEGC
Sbjct: 481 MTPEVKLHILQGRQHSSQLKDEQRKKEREMINELLSVKEILRDAKQCPSCKMAISRTEGC 540

Query: 541 NKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQ----QWEERMNARQVLGQIQ 600
           NKMVCNNCGQYFCYRC++AIDGY+HFR+ +CELFPQE IQ     WE R+N RQV+GQIQ
Sbjct: 541 NKMVCNNCGQYFCYRCNQAIDGYEHFRDSACELFPQEMIQNWEWDWEARLNQRQVVGQIQ 600

Query: 601 AELFPENGRPCPNCGQLNAKVSRS 604
           AELF + G  CP C Q+N KV  +
Sbjct: 601 AELFADRGHSCPICRQINVKVGNN 624

BLAST of MELO3C003429T2 vs. TrEMBL
Match: A0A061G7P6_THECC (NDR1/HIN1-like 8, putative isoform 1 OS=Theobroma cacao GN=TCM_016558 PE=4 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 1.1e-210
Identity = 373/644 (57.92%), Postives = 460/644 (71.43%), Query Frame = 1

Query: 1   MRKSGRGGRWTDHNRN----QEVWVAK--------------HTNHSHN---HASSSASQ- 60
           MR++ RG R   H+       E W  K              H     N    +S S SQ 
Sbjct: 1   MRRTRRGSRQGHHHHQPLQADETWSVKPLHDTPQPPQQDPHHQEEESNLNGPSSPSISQQ 60

Query: 61  ---PSHLSNPPPTGRNFSYEHRHPRRPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAK 120
              P   S P  T RN ++++R     +P+ V K E+ S    + S   ++      V +
Sbjct: 61  LQSPDFTSKPSRTHRNPNWKYRRGHVAKPRFVKKSELISSKSELSS---EDNIASLSVVE 120

Query: 121 P-NCEVKQQTPGNS---------------SSAEAADDVYSRLEMLQRSSEEPVLSEEQLS 180
             NC+ ++     +                S  +ADDV SR E L+   +EP LSEEQL 
Sbjct: 121 EVNCDQEENKGSEAFVSKIDGDGEEIEGDESQNSADDVVSRFEELRLRVDEPELSEEQLR 180

Query: 181 INDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIK 240
           INDQLQEDELLA++SIYGENV+ILD+  G R  +I IH+E  G+  IT KLNSS+ +   
Sbjct: 181 INDQLQEDELLAMKSIYGENVFILDKEMGLRSLKIHIHLEGSGETIITTKLNSSNDVG-G 240

Query: 241 SPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIW 300
           S  SD+FS SF V+YL PIVLTCLLP+SYPSHLPPY+TIS++WL SARIS LCS LD IW
Sbjct: 241 SETSDDFSYSFKVQYLPPIVLTCLLPRSYPSHLPPYFTISVQWLHSARISDLCSQLDLIW 300

Query: 301 SEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAII 360
            EQ GQEV+Y+W +WLQ  SL+YL  DKEIMLGPY   ++G+ RAVSGSVSP+VD+P I 
Sbjct: 301 KEQEGQEVIYRWADWLQNFSLSYLGCDKEIMLGPYGIENNGERRAVSGSVSPDVDVPFIR 360

Query: 361 NYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKL 420
           +Y+++   E F  +L EC IC S++AG  F++LPC+H+FC KC++TYS MH+ EGTV+KL
Sbjct: 361 SYNDEICHENFLESLHECSICFSEYAGIDFIRLPCQHFFCCKCIKTYSDMHITEGTVNKL 420

Query: 421 NCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHD 480
            CP+AKC   VPPGLLK+LLGDE +ERWES+ML KTLESMSDVVYCPRCETPC+ED D  
Sbjct: 421 LCPEAKCGGTVPPGLLKRLLGDEGYERWESLMLQKTLESMSDVVYCPRCETPCIEDEDQH 480

Query: 481 AQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELIS 540
           AQCSKC+FSFCTLC ERRHVGIECMTPE+KLR+L+ERQNSSQL   Q+ KEREMINE++S
Sbjct: 481 AQCSKCFFSFCTLCRERRHVGIECMTPELKLRILQERQNSSQLNQAQKHKEREMINEILS 540

Query: 541 VKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQ 600
           +KEI+RDAKQCPSCKMAIS+TEGCNKMVC NCGQYFCYRC+ AI+GYDHFR+G+CELFPQ
Sbjct: 541 MKEIMRDAKQCPSCKMAISKTEGCNKMVCENCGQYFCYRCNSAINGYDHFRDGACELFPQ 600

Query: 601 EAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKVSRS 604
           E I++WEER+NARQVLGQ+ A+LF E G+PCPNC QLNAKV  +
Sbjct: 601 EMIREWEERLNARQVLGQVHAQLFAERGQPCPNCRQLNAKVGNN 640

BLAST of MELO3C003429T2 vs. TrEMBL
Match: W9R3E3_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_017770 PE=4 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 4.1e-210
Identity = 368/635 (57.95%), Postives = 451/635 (71.02%), Query Frame = 1

Query: 1   MRKSGRGGRWTDHNR--NQEVWVAK----HTNHSHNH--------------ASSSASQPS 60
           MRKS  GG   DH+     E W  K    H  H+ NH               +++  +P 
Sbjct: 1   MRKSRGGGGKQDHHSIIADETWAPKPLDQHHLHNPNHEVGPQPPSTSHLEPTATTTHEPP 60

Query: 61  HLSNPPPTGRN--FSYEHRHPRRPRPQPVHKPEVTSLADGIESFTFDE----KPVDFDVA 120
             + PP   RN  ++  +R  R  +   V K EV SL   + S T DE    K    D+A
Sbjct: 61  IFNLPPARRRNPRWASRNRRGRGAKRGSVEKSEVGSLDSEVGSLTIDEAVDGKEESGDLA 120

Query: 121 KPNCEV----KQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELL 180
             N EV    K+    ++   +  DD+  RLE L+   EEP LSEEQL IN Q Q DELL
Sbjct: 121 MVNDEVGEVRKEDCESSNDKDDDDDDIMGRLEKLRLGVEEPELSEEQLRINSQSQMDELL 180

Query: 181 ALESIYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLE-IKSPNSDEFSCS 240
            +ESIYG+NV+ILD   G + FQ+ IHIE P  IT+T KLNSS  ++ I + N D+FS S
Sbjct: 181 VMESIYGDNVFILDREDGLQSFQVHIHIEAPNGITVTTKLNSSGDVKTIDNNNRDDFSYS 240

Query: 241 FNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVY 300
           F V+YL PI+L CLLPKSYPSHLPPY+T+S++WLDS +++ LCS+L  IW EQ GQEV+Y
Sbjct: 241 FKVQYLPPILLICLLPKSYPSHLPPYFTLSVQWLDSIKVANLCSMLGTIWKEQHGQEVIY 300

Query: 301 QWVEWLQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEE 360
           QWVEWL  S+L+YL  D EI+LGPY      D RAVSGSVSP+ D+P I +++++R  E 
Sbjct: 301 QWVEWLNSSALSYLGFDSEIILGPYGIRRVEDMRAVSGSVSPDTDVPFIRSFNDERLLEN 360

Query: 361 FCMNLQECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVM 420
           F  NLQECCIC   + GT FV+LPC H+FCWKCM+ YS MH+ EGTVSKL CP+AKC VM
Sbjct: 361 FRQNLQECCICFEDYPGTDFVRLPCHHFFCWKCMKAYSDMHISEGTVSKLQCPEAKCGVM 420

Query: 421 VPPGLLKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSF 480
           VPP LLK+LL  EE+E WES+ML KTLESMSDV YCPRCETPC+ED D  AQCSKC+FSF
Sbjct: 421 VPPSLLKRLLSQEEYEWWESLMLQKTLESMSDVAYCPRCETPCIEDEDQHAQCSKCFFSF 480

Query: 481 CTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQ 540
           CTLC E+RH+G++CMTPE+KL++LE+RQ SSQL  EQ+R+E EMINEL+SVKEILRDAK 
Sbjct: 481 CTLCREKRHLGVQCMTPELKLQILEDRQKSSQLRDEQKRRELEMINELLSVKEILRDAKN 540

Query: 541 CPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERM 600
           CPSCKMAISRTEGCNKM C+NCGQYFCYRC KAIDGYDHFR+G+C+L+P + IQ+WEERM
Sbjct: 541 CPSCKMAISRTEGCNKMACSNCGQYFCYRCGKAIDGYDHFRDGACDLYPPQMIQEWEERM 600

Query: 601 NARQVLGQIQAELF--PENGRPCPNCGQLNAKVSR 603
           NARQVLGQ+QA+LF     G+ CPNC Q NAKVS+
Sbjct: 601 NARQVLGQVQAQLFVGQGQGQLCPNCRQFNAKVSK 635

BLAST of MELO3C003429T2 vs. TrEMBL
Match: V4T2M3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011251mg PE=4 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 6.6e-208
Identity = 367/624 (58.81%), Postives = 450/624 (72.12%), Query Frame = 1

Query: 1   MRKSGRGG-RWTDHNRNQEVWVAK----------------HTNHSHNHASSSASQPSHLS 60
           MR++ RGG R  +H +N E    K                H  HS +  S+S  QP++  
Sbjct: 5   MRRTRRGGGRPRNHLQNDEDMTVKPLNDAPPAVPPSQQKRHQQHSLDSPSTS-DQPNNEP 64

Query: 61  NPPPTGR-NFSYEHRHPRRPRPQPVHKPEVTS---LADGIESFTFDEKPVDFDVAKPNCE 120
            P   G   +    R  R  +P+ V K E      L D + S +  E+       K   +
Sbjct: 65  IPKYQGNLRWVSRRRRARVAKPKFVKKTESREEKLLNDEVGSLSIGEE------VKEKQD 124

Query: 121 VKQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGEN 180
            ++  P NS+     +DV S L  L  S EEP L  EQL INDQ Q DELLALESIYG+N
Sbjct: 125 EEEGLPSNSN----VNDVESILRELNSSFEEPELEAEQLRINDQSQGDELLALESIYGDN 184

Query: 181 VYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIV 240
           V+ILD  +  R  QI +H+E    +T+TAKLNSS+ L+ +S +SD+FS SF V+YL PIV
Sbjct: 185 VFILDRQRDLRSCQIHVHVETLDGLTVTAKLNSSTDLDARSESSDDFSYSFKVQYLPPIV 244

Query: 241 LTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSS 300
           LTCLLPKSYPSHLPPY+TIS RWL+S +IS LCS+L+ IW +Q GQE++YQW+EWLQ SS
Sbjct: 245 LTCLLPKSYPSHLPPYFTISARWLNSTKISNLCSMLESIWIDQPGQEILYQWIEWLQNSS 304

Query: 301 LAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCI 360
           L+YL   +EI+LGPY     GD RA+S SVSP+VDIP+I +Y+++R  E F  +  EC I
Sbjct: 305 LSYLGFSEEIVLGPYGVARIGDRRAISESVSPDVDIPSIRSYNDERCHENFLKSFHECRI 364

Query: 361 CLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLL 420
           C S+ AGT FV+LPC H+FCWKCM+TY  +H+ EGTVSKL CPDAKC  MVPP LLK+LL
Sbjct: 365 CFSELAGTDFVRLPCHHFFCWKCMKTYLDIHISEGTVSKLQCPDAKCGGMVPPSLLKKLL 424

Query: 421 GDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHV 480
           GDEEFERWES+ML KTLESMSDV YCPRCETPC+ED +  AQCSKC++SFCTLC ERRHV
Sbjct: 425 GDEEFERWESLMLQKTLESMSDVAYCPRCETPCIEDEEQHAQCSKCFYSFCTLCRERRHV 484

Query: 481 GIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISR 540
           G+ CMTPE+KL +L+ERQNSSQ+   Q+++EREMINEL+SVKEILRDAKQCPSCKMAISR
Sbjct: 485 GVVCMTPEIKLHILQERQNSSQVKEGQKQREREMINELLSVKEILRDAKQCPSCKMAISR 544

Query: 541 TEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQ 600
           TEGCNK+VCNNCGQYFCYRC+KAIDGYDHFR G+CELFP E I+ WEER+NARQV+ QIQ
Sbjct: 545 TEGCNKIVCNNCGQYFCYRCNKAIDGYDHFRTGTCELFPLEMIRDWEERLNARQVVAQIQ 604

Query: 601 AELFPENGRPCPNCGQLNAKVSRS 604
           A++F E+G  CPNC Q NAKV  +
Sbjct: 605 ADMFDEHGLSCPNCRQFNAKVGNN 617

BLAST of MELO3C003429T2 vs. TAIR10
Match: AT1G32340.1 (AT1G32340.1 NDR1/HIN1-like 8)

HSP 1 Score: 666.8 bits (1719), Expect = 1.3e-191
Identity = 332/614 (54.07%), Postives = 417/614 (67.92%), Query Frame = 1

Query: 25  TNHSHNHASSSASQP----SHLSNPPPTGRNFSYEHRHPRRPRPQPVHKPEVT------- 84
           T+ S   A  S SQP     H + P  + RN           +P+PV KPEV        
Sbjct: 43  TDLSSAAAFPSTSQPIQNLDHSTKPSKSHRN---RRSRGSNSKPRPVEKPEVNFAESDVV 102

Query: 85  -SLADGIE---------SFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSR--- 144
             LAD +          S   +EK         NCE  +     S       D Y     
Sbjct: 103 DCLADELSRLKVKQNSHSVNMEEKFHHSSSDHSNCEESELKRAESEEIADGVDEYETKED 162

Query: 145 -----LEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIF 204
                L+ L+ S  EP L+EEQL +NDQLQEDELLAL  IYG N++I D Y   R FQ+ 
Sbjct: 163 IMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELLALGYIYGGNMFIFDRYNDMRYFQVH 222

Query: 205 IHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPY 264
           +++E   + TI+ KLN  +     S  S++F  SF  ++L PIVL CLLPK+YPSHLPPY
Sbjct: 223 VNVEATSEYTISTKLNLQAD---SSKESEDFLYSFKAQHLPPIVLKCLLPKAYPSHLPPY 282

Query: 265 YTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYD 324
           + IS++W++  +IS LCS LD +WSEQ GQEV+YQW +WLQ SS+++L  D EI+LGPY 
Sbjct: 283 FLISVQWMNPDKISSLCSKLDSLWSEQPGQEVLYQWTDWLQNSSISHLGFDNEIVLGPYG 342

Query: 325 KGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCK 384
              S D RAVSGS SP+ DIP I +YD+++R + F  +L ECCIC S+ AG  FVKLPC+
Sbjct: 343 VTCSRDKRAVSGSRSPDSDIPYIRSYDDEKRHDSFLQSLHECCICFSESAGIDFVKLPCQ 402

Query: 385 HYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKT 444
           H+FC KCM+TY+ +HV EGTV+KL CPD+KC   VPPG+LK+LLGDE +ERWE++ML KT
Sbjct: 403 HFFCLKCMKTYTDIHVTEGTVNKLKCPDSKCGETVPPGILKRLLGDEAYERWETLMLQKT 462

Query: 445 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 504
           LESM+DV YCPRCETPC+ED +  A C KCYFSFCTLC E+RHVG+ CM+PE++L++L+E
Sbjct: 463 LESMTDVAYCPRCETPCIEDEEQLALCFKCYFSFCTLCKEKRHVGVACMSPELRLQILQE 522

Query: 505 RQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 564
           RQ+SS+LG EQRRKE+EMINE++SVK I++ AKQCPSCK+AISRT GCNKMVCNNCGQYF
Sbjct: 523 RQDSSRLGEEQRRKEKEMINEIMSVKVIMKSAKQCPSCKIAISRTGGCNKMVCNNCGQYF 582

Query: 565 CYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAEL------FPENGRP 604
           CYRC++AI GY+HF EG CELFPQEAIQ+W E MN RQV+GQIQA+L      FP+ G+ 
Sbjct: 583 CYRCNEAITGYEHFSEGKCELFPQEAIQEWNEMMNERQVIGQIQAQLFAQGGQFPQRGQL 642

BLAST of MELO3C003429T2 vs. TAIR10
Match: AT3G14250.1 (AT3G14250.1 RING/U-box superfamily protein)

HSP 1 Score: 99.0 bits (245), Expect = 1.1e-20
Identity = 58/212 (27.36%), Postives = 94/212 (44.34%), Query Frame = 1

Query: 337 CCICLSQFAGTKFVK--LPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGL 396
           C IC+ +   +   +    C H +C  C   Y +  +KE   S++ CPD +C  ++ P  
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENA-SRIKCPDVECTRLIEPYT 154

Query: 397 LKQLLGDEEFERWESMMLTKTLESMSDVVYCP--RCETPCLEDVDHDA-----QCSKCYF 456
            + L+  + F+RWE + L ++L S  D  YCP   C    + + + DA     +C  C+ 
Sbjct: 155 CRDLIPKDVFDRWEKI-LCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHR 214

Query: 457 SFCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDA 516
            FC  C    H GI C          +E Q       +   ++  ++ ++   K+     
Sbjct: 215 LFCVQCKVTWHAGIGC----------DEFQRFGNTKKKSSDEDDALLIQMAKNKQW---- 274

Query: 517 KQCPSCKMAISRTEGCNKMVCNNCGQYFCYRC 540
           ++CPSCK  + + EGC  + C  CG  FCY C
Sbjct: 275 RRCPSCKFYVDKVEGCQHIKC-RCGYQFCYGC 289

BLAST of MELO3C003429T2 vs. TAIR10
Match: AT3G53690.1 (AT3G53690.1 RING/U-box superfamily protein)

HSP 1 Score: 86.3 bits (212), Expect = 7.3e-17
Identity = 56/221 (25.34%), Postives = 99/221 (44.80%), Query Frame = 1

Query: 336 ECCICLSQFAGTKFVKLP-CKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGL 395
           +C IC+   +  +  ++  C H++C  C+  Y +  +++  +S + CP + C   + P  
Sbjct: 114 DCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILS-IECPVSGCSGRLEPDQ 173

Query: 396 LKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDH------DAQCSKCYFS 455
            +Q+L  E F+RW    L + +   S   YCP  +   L  ++       D++C  C+  
Sbjct: 174 CRQILPKEVFDRWGDA-LCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRM 233

Query: 456 FCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAK 515
            C  C  + H  + C          EE Q   +L + +R ++  ++  +   K+     K
Sbjct: 234 VCVECGTQWHPEMTC----------EEFQ---KLAANERGRDDILLATMAKQKKW----K 293

Query: 516 QCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHF 550
           +CPSCK  I +++GC  M C  CG  FCY C      + H+
Sbjct: 294 RCPSCKFYIEKSQGCLYMKC-RCGLAFCYNCGTPSRDHTHY 314

BLAST of MELO3C003429T2 vs. TAIR10
Match: AT4G01020.1 (AT4G01020.1 helicase domain-containing protein / IBR domain-containing protein / zinc finger protein-related)

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-11
Identity = 56/228 (24.56%), Postives = 90/228 (39.47%), Query Frame = 1

Query: 336  ECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLL 395
            EC ICLS+      ++  C H FC  C+       ++      + C    C   +    +
Sbjct: 1560 ECPICLSEVDDGYSLE-GCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1619

Query: 396  KQLLGDEEFERWESMMLTKTLESMSDVV-YCPRCETPCLEDVDHDAQ------CSKCYFS 455
            + LL  E+ +   S  L+  + S    + +C   + P +  V    +      C  C+  
Sbjct: 1620 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYRVAGPQESGEPFICGACHSE 1679

Query: 456  FCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILR--D 515
             CT C    H  I C                     E+ +K +E  N  +S+K+  +  D
Sbjct: 1680 TCTRCHLEYHPLITC---------------------ERYKKFKE--NPDLSLKDWAKGKD 1739

Query: 516  AKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRC----SKAIDGYDHFR 551
             K+CP CK  I +T+GCN + C  CG++ C+ C    ++A   Y H R
Sbjct: 1740 VKECPICKSTIEKTDGCNHLQC-RCGKHICWTCLDVFTQAEPCYAHLR 1762

BLAST of MELO3C003429T2 vs. TAIR10
Match: AT5G10370.1 (AT5G10370.1 helicase domain-containing protein / IBR domain-containing protein / zinc finger protein-related)

HSP 1 Score: 67.0 bits (162), Expect = 4.6e-11
Identity = 52/213 (24.41%), Postives = 83/213 (38.97%), Query Frame = 1

Query: 336  ECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLL 395
            EC ICLS+      ++  C H FC  C+       ++      + C    C   +    +
Sbjct: 1563 ECPICLSEVDDGYSLE-GCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVLADM 1622

Query: 396  KQLLGDEEFERWESMMLTKTLESMSDVV-YCPRCETPCLEDVDHDAQ------CSKCYFS 455
            + LL  E+ +   S  L+  + S      +C   + P +  V    +      C  C+  
Sbjct: 1623 RALLSQEKLDELFSASLSSFVTSSDGKFRFCSTPDCPSVYRVAGPQESGEPFICGACHSE 1682

Query: 456  FCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILR--D 515
             CT C    H  I C                     E+ +K +E  N  +S+K+  +  +
Sbjct: 1683 ICTRCHLEYHPLITC---------------------ERYKKFKE--NPDLSLKDWAKGKN 1742

Query: 516  AKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRC 540
             K+CP CK  I +T+GCN M C  CG++ C+ C
Sbjct: 1743 VKECPICKSTIEKTDGCNHMKC-RCGKHICWTC 1750

BLAST of MELO3C003429T2 vs. NCBI nr
Match: gi|659132069|ref|XP_008466000.1| (PREDICTED: E3 ubiquitin-protein ligase RNF14 [Cucumis melo])

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 600/603 (99.50%), Postives = 601/603 (99.67%), Query Frame = 1

Query: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR 60
           MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR
Sbjct: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR 60

Query: 61  RPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSR 120
           RPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSR
Sbjct: 61  RPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSR 120

Query: 121 LEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN 180
           LEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN
Sbjct: 121 LEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN 180

Query: 181 PGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISI 240
           PGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISI
Sbjct: 181 PGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISI 240

Query: 241 RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSG 300
           RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSG
Sbjct: 241 RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSG 300

Query: 301 DTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCW 360
           DTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCW
Sbjct: 301 DTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCW 360

Query: 361 KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS 420
           KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS
Sbjct: 361 KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS 420

Query: 421 DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS 480
           DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS
Sbjct: 421 DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS 480

Query: 481 QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS 540
           QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS
Sbjct: 481 QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS 540

Query: 541 KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKV 600
           KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKV
Sbjct: 541 KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKV 600

Query: 601 SRS 604
             +
Sbjct: 601 GNN 603

BLAST of MELO3C003429T2 vs. NCBI nr
Match: gi|449436832|ref|XP_004136196.1| (PREDICTED: E3 ubiquitin-protein ligase RNF14 [Cucumis sativus])

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 572/603 (94.86%), Postives = 584/603 (96.85%), Query Frame = 1

Query: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR 60
           MRKSGRGGRWTDHNRNQEVWVAKHTN SHNHAS+SASQPSHLSNP PT RNF+Y+HR PR
Sbjct: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNQSHNHASTSASQPSHLSNPSPTARNFNYDHRIPR 60

Query: 61  RPRPQPVHKPEVTSLADGIESFTFDEKPVDFDVAKPNCEVKQQTPGNSSSAEAADDVYSR 120
           RPRP PVHKPEVT+LADGIE+FTFDEKPVDFDVAKPNCEV   TPGNSS  EA DDVYSR
Sbjct: 61  RPRPHPVHKPEVTALADGIETFTFDEKPVDFDVAKPNCEV---TPGNSSLTEAEDDVYSR 120

Query: 121 LEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN 180
           LEMLQRSSEEP LSEEQLSIN+QLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN
Sbjct: 121 LEMLQRSSEEPELSEEQLSINNQLQEDELLALESIYGENVYILDEYKGQRRFQIFIHIEN 180

Query: 181 PGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISI 240
           PGDITITAKLNSSSSLEIKSPNSDEFSCSF+VKYLNPIVLTCLLPK YPSHLPPYYTISI
Sbjct: 181 PGDITITAKLNSSSSLEIKSPNSDEFSCSFDVKYLNPIVLTCLLPKVYPSHLPPYYTISI 240

Query: 241 RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSG 300
           RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQ DKEIMLGPYDKGHSG
Sbjct: 241 RWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQSDKEIMLGPYDKGHSG 300

Query: 301 DTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCW 360
           DTRAVSG VSPEVD+PAIINYDNQRRDE+FCMNLQECCICLSQ+AG KFV+LPCKHYFCW
Sbjct: 301 DTRAVSGIVSPEVDVPAIINYDNQRRDEDFCMNLQECCICLSQYAGAKFVRLPCKHYFCW 360

Query: 361 KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS 420
           KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS
Sbjct: 361 KCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMS 420

Query: 421 DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS 480
           DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS
Sbjct: 421 DVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSS 480

Query: 481 QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS 540
           QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS
Sbjct: 481 QLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCS 540

Query: 541 KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKV 600
           KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENG PCPNCGQLNAKV
Sbjct: 541 KAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGHPCPNCGQLNAKV 600

Query: 601 SRS 604
             +
Sbjct: 601 GNN 600

BLAST of MELO3C003429T2 vs. NCBI nr
Match: gi|645265559|ref|XP_008238208.1| (PREDICTED: E3 ubiquitin-protein ligase RNF14 [Prunus mume])

HSP 1 Score: 755.7 bits (1950), Expect = 6.1e-215
Identity = 352/497 (70.82%), Postives = 412/497 (82.90%), Query Frame = 1

Query: 107 NSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEY 166
           + SS    DDV SRLE LQ   EEP LS+EQL I+DQ QEDELLA+ESIYG++ +I +  
Sbjct: 72  SESSKGEDDDVLSRLEELQLGVEEPRLSDEQLRIDDQAQEDELLAMESIYGDDFFIFERQ 131

Query: 167 KGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPK 226
           +G+R FQI IH+E PG+I +T KLNSSS L+ +S  SDEFS SF  ++L PI+LTCLLPK
Sbjct: 132 RGRRSFQIHIHVEAPGEIAVTTKLNSSSDLKTRSEGSDEFSYSFKAQHLPPIMLTCLLPK 191

Query: 227 SYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLD 286
           SYPSHLPPY+TIS++WL S++IS LCS LD IW EQ GQEV+YQWVEWLQ SSL+YL  D
Sbjct: 192 SYPSHLPPYFTISVQWLHSSKISHLCSELDSIWREQPGQEVMYQWVEWLQNSSLSYLSFD 251

Query: 287 KEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAG 346
           KEI+LGPY    +GD RAV+GSVSP+VDIP I +Y++ R  E F  NL EC IC S+FAG
Sbjct: 252 KEIILGPYGVRDAGDRRAVTGSVSPDVDIPFIRSYNDDRLHENFHKNLHECRICFSEFAG 311

Query: 347 TKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFER 406
           T+FV+LPC+H+FCWKCM+TYS MHVKEGTVSKL CPDAKC  M+PPGLLK+LL  EEFER
Sbjct: 312 TEFVRLPCQHFFCWKCMKTYSDMHVKEGTVSKLQCPDAKCGGMIPPGLLKRLLDVEEFER 371

Query: 407 WESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTP 466
           WES++L KTLE+MSDVVYCPRCE PC+ED D  AQCSKC+FSFCTLC ERRHVGI+CMTP
Sbjct: 372 WESLILIKTLEAMSDVVYCPRCEIPCIEDEDQHAQCSKCFFSFCTLCKERRHVGIQCMTP 431

Query: 467 EMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKM 526
           EMKL +L+ERQN  QL  +QR KERE INE++SV+EILRDAKQCPSCKMAISRTEGCNKM
Sbjct: 432 EMKLHILQERQNFGQLNDDQRHKEREKINEILSVREILRDAKQCPSCKMAISRTEGCNKM 491

Query: 527 VCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPEN 586
           VC NCGQYFCYRC KAI+GYDHFR+G+CELFPQEAIQ WE +MN RQVLGQIQAELF  +
Sbjct: 492 VCKNCGQYFCYRCDKAIEGYDHFRDGACELFPQEAIQNWEAQMNPRQVLGQIQAELFAAH 551

Query: 587 GRPCPNCGQLNAKVSRS 604
           G+ CPNCGQ NAK+  +
Sbjct: 552 GQLCPNCGQFNAKIGNN 568

BLAST of MELO3C003429T2 vs. NCBI nr
Match: gi|1009158997|ref|XP_015897576.1| (PREDICTED: E3 ubiquitin-protein ligase RNF14-like [Ziziphus jujuba])

HSP 1 Score: 752.7 bits (1942), Expect = 5.2e-214
Identity = 363/569 (63.80%), Postives = 437/569 (76.80%), Query Frame = 1

Query: 56  HRHPRRPRPQPVHKPEVTSLADGIES-------FTFDEKPVDF--------------DVA 115
           +R  R  +PQ V K EV S    + S       F+F E+  +               ++A
Sbjct: 123 NRRARGVKPQFVEKSEVGSSNSEVGSANGEVGGFSFGERASEEQEDHEETQEQEKQENLA 182

Query: 116 KPNCEVKQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALES 175
             N   +++      S++  DD+ SRL  LQ  +EEP LSEEQL INDQLQEDELLA+ES
Sbjct: 183 AKNGMREEKIEEMFESSQEVDDIVSRLAELQLGAEEPELSEEQLRINDQLQEDELLAMES 242

Query: 176 IYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKY 235
           IYGEN++ILD+ +  R FQI IHIE P +IT+   L+SS  L+  + NSD FS SF V+Y
Sbjct: 243 IYGENLFILDKQRDLRSFQIHIHIELPNEITVITNLSSSGDLKTITDNSDYFSYSFKVQY 302

Query: 236 LNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEW 295
           L PIVLTCLLPKSYPSHLPP++T+S+RWLDS RIS LCS+LD IW EQ+GQEV+YQWVEW
Sbjct: 303 LPPIVLTCLLPKSYPSHLPPHFTLSVRWLDSIRISNLCSMLDSIWMEQMGQEVIYQWVEW 362

Query: 296 LQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNL 355
           L  SSL++L  D+E+MLGPY   H  D RAVS  VSP+VDIP I NY+N+R  E F  NL
Sbjct: 363 LHSSSLSHLAFDEEMMLGPYGVRHIEDRRAVSECVSPDVDIPFIRNYNNERLHENFQTNL 422

Query: 356 QECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGL 415
            ECCIC S++AGT+FV+LPC H+FC+KCM+TYS +HV EGT+ KL CP+A C  M+PP L
Sbjct: 423 HECCICFSEYAGTEFVRLPCLHFFCFKCMKTYSDIHVNEGTIMKLQCPNANCGGMLPPSL 482

Query: 416 LKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCS 475
           LK+LLGD E+ERWES+ML +TLESMSDV YCPRCETPC+ED D  AQC KCYFSFCTLC+
Sbjct: 483 LKRLLGDGEYERWESLMLQRTLESMSDVAYCPRCETPCIEDEDQHAQCPKCYFSFCTLCN 542

Query: 476 ERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCK 535
           ERRHVG ECMTPEMKL++LEERQNS+QL   Q+++ER+MINEL+SVKEILRD+KQCPSCK
Sbjct: 543 ERRHVGGECMTPEMKLKILEERQNSTQLKDSQKKRERDMINELLSVKEILRDSKQCPSCK 602

Query: 536 MAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQV 595
           +AISRTEGCNKMVCNNCGQYFCYRC+KAIDGYDHFREGSC+LFPQE I+ WEE +N RQV
Sbjct: 603 IAISRTEGCNKMVCNNCGQYFCYRCNKAIDGYDHFREGSCDLFPQEMIRNWEEHINPRQV 662

Query: 596 LGQIQAELFPENGRPCPNCGQLNAKVSRS 604
           LGQIQAELF E+G+ CPNCGQ N KV  +
Sbjct: 663 LGQIQAELFAEHGQLCPNCGQFNGKVGNN 691

BLAST of MELO3C003429T2 vs. NCBI nr
Match: gi|661883422|emb|CDP13066.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 747.7 bits (1929), Expect = 1.7e-212
Identity = 372/639 (58.22%), Postives = 458/639 (71.67%), Query Frame = 1

Query: 2   RKSGRGGRWTDHNRNQEVWVAKHTNHSHN--------HASSSAS---QPSHLSNPPPTGR 61
           R+ G+   +  H +N E W  K     HN         A S++    + +H   P  T +
Sbjct: 9   RRGGKNSHFYQH-QNDENWTLKRVQ-DHNLPPQFDQQFAPSNSELNVEHNHFEQPTTTSK 68

Query: 62  NFSYE-----------HRHPRRPRPQPVHKPEVTSLADGIESFTFDEKPVDF-------- 121
           N  Y            +R P+  +PQ V K EV+SL  GI+    DEK  DF        
Sbjct: 69  NQKYRQNRRNPGWGLRNRRPQAVKPQFVKKSEVSSL--GIDERN-DEKREDFRDGLGEEL 128

Query: 122 -------DVAKPNCEVKQQTPGNSSSAEAADDVYSRLEMLQRSSEEPVLSEEQLSINDQL 181
                  +V +   EV+++  G+  + E  D V  RLE L  S++E  LSEEQL INDQ 
Sbjct: 129 EKIASKSEVVEGKDEVEEKIEGSGLNEEDVDVVQKRLEELLLSAQEAELSEEQLRINDQA 188

Query: 182 QEDELLALESIYGENVYILDEYKGQRRFQIFIHIENPGDITITAKLNSSSSLEIKSPNSD 241
           QEDELLA+ESIYG+N++ LD+  G R FQI IHIE P +I++  KLNSS+ +   + +S 
Sbjct: 189 QEDELLAMESIYGDNIFFLDKQNGLRSFQIHIHIETPSEISVAVKLNSSA-VSKANDDSS 248

Query: 242 EFSCSFNVKYLNPIVLTCLLPKSYPSHLPPYYTISIRWLDSARISRLCSVLDGIWSEQVG 301
           EFS SF V+YL PI+LTC+LPKSYPSHLPP++TIS +WL S +IS LC+VLD IW+EQ G
Sbjct: 249 EFSYSFKVEYLPPIILTCILPKSYPSHLPPHFTISAQWLSSTKISDLCNVLDSIWNEQSG 308

Query: 302 QEVVYQWVEWLQMSSLAYLQLDKEIMLGPYDKGHSGDTRAVSGSVSPEVDIPAIINYDNQ 361
           QEV+YQWVE +  S L++L  D+EI+LGPY      D RA+SGSVSPEVDIP + +Y ++
Sbjct: 309 QEVIYQWVESIHSSCLSFLGFDQEIILGPYAVKQERDRRAISGSVSPEVDIPYMKSYSDE 368

Query: 362 RRDEEFCMNLQECCICLSQFAGTKFVKLPCKHYFCWKCMETYSSMHVKEGTVSKLNCPDA 421
           +R E F  N+ ECCIC S+FAGT+FV+LPC+H+FCWKCM+TYS +HVKEGTVSKL C DA
Sbjct: 369 QRHENFSKNIHECCICFSEFAGTEFVRLPCQHFFCWKCMKTYSDIHVKEGTVSKLQCADA 428

Query: 422 KCDVMVPPGLLKQLLGDEEFERWESMMLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSK 481
           KC  M+PPGLL++LLGDEEFERWESM L KTLESM+DV YCPRCET C+ED +  AQCSK
Sbjct: 429 KCGGMIPPGLLRRLLGDEEFERWESMTLQKTLESMTDVTYCPRCETICIEDEEQHAQCSK 488

Query: 482 CYFSFCTLCSERRHVGIECMTPEMKLRLLEERQNSSQLGSEQRRKEREMINELISVKEIL 541
           CYFSFCTLC E+RHVG+ CMTPEMKL +L+ERQNSSQL  +QRR+E+E+IN+L+SVKEI 
Sbjct: 489 CYFSFCTLCREKRHVGVACMTPEMKLLVLQERQNSSQLKDDQRRREKEIINDLLSVKEIN 548

Query: 542 RDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGSCELFPQEAIQQ 601
             AKQCPSCKMAISRTEGCNKMVC NCG YFCYRC+K IDGYDHFR+GSCELFP E IQ 
Sbjct: 549 ISAKQCPSCKMAISRTEGCNKMVCGNCGHYFCYRCNKEIDGYDHFRDGSCELFPPETIQH 608

Query: 602 WEERMNARQVLGQIQAELFPENGRPCPNCGQLNAKVSRS 604
           WEERMNARQV+GQIQAE+FPE G  CPNCGQ+N K   +
Sbjct: 609 WEERMNARQVVGQIQAEMFPERGHSCPNCGQINLKAGNN 641

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RNF14_MOUSE2.3e-5731.67E3 ubiquitin-protein ligase RNF14 OS=Mus musculus GN=Rnf14 PE=1 SV=2[more]
RNF14_HUMAN4.4e-5631.52E3 ubiquitin-protein ligase RNF14 OS=Homo sapiens GN=RNF14 PE=1 SV=1[more]
ITT1_SCHPO3.6e-3426.40E3 ubiquitin-protein ligase itt1 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
ITT1_YEAST2.8e-2628.84Translation termination inhibitor protein ITT1 OS=Saccharomyces cerevisiae (stra... [more]
Y5521_DICDI2.5e-1928.23Uncharacterized protein DDB_G0292642 OS=Dictyostelium discoideum GN=DDB_G0292642... [more]
Match NameE-valueIdentityDescription
A0A068UXB6_COFCA1.2e-21258.22Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037831001 PE=4 SV=1[more]
D7TTU0_VITVI2.8e-21158.97Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0012g01750 PE=4 SV=... [more]
A0A061G7P6_THECC1.1e-21057.92NDR1/HIN1-like 8, putative isoform 1 OS=Theobroma cacao GN=TCM_016558 PE=4 SV=1[more]
W9R3E3_9ROSA4.1e-21057.95E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_017770 PE=4 SV=1[more]
V4T2M3_9ROSI6.6e-20858.81Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011251mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G32340.11.3e-19154.07 NDR1/HIN1-like 8[more]
AT3G14250.11.1e-2027.36 RING/U-box superfamily protein[more]
AT3G53690.17.3e-1725.34 RING/U-box superfamily protein[more]
AT4G01020.11.2e-1124.56 helicase domain-containing protein / IBR domain-containing protein /... [more]
AT5G10370.14.6e-1124.41 helicase domain-containing protein / IBR domain-containing protein /... [more]
Match NameE-valueIdentityDescription
gi|659132069|ref|XP_008466000.1|0.0e+0099.50PREDICTED: E3 ubiquitin-protein ligase RNF14 [Cucumis melo][more]
gi|449436832|ref|XP_004136196.1|0.0e+0094.86PREDICTED: E3 ubiquitin-protein ligase RNF14 [Cucumis sativus][more]
gi|645265559|ref|XP_008238208.1|6.1e-21570.82PREDICTED: E3 ubiquitin-protein ligase RNF14 [Prunus mume][more]
gi|1009158997|ref|XP_015897576.1|5.2e-21463.80PREDICTED: E3 ubiquitin-protein ligase RNF14-like [Ziziphus jujuba][more]
gi|661883422|emb|CDP13066.1|1.7e-21258.22unnamed protein product [Coffea canephora][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR002867IBR_dom
IPR006575RWD-domain
IPR013083Znf_RING/FYVE/PHD
IPR016135UBQ-conjugating_enzyme/RWD
IPR017907Znf_RING_CS
Vocabulary: Molecular Function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0005515protein binding
GO:0008270zinc ion binding
Vocabulary: Biological Process
TermDefinition
GO:0016567protein ubiquitination
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006499 N-terminal protein myristoylation
biological_process GO:0006487 protein N-linked glycosylation
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0016874 ligase activity
molecular_function GO:0003676 nucleic acid binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C003429MELO3C003429gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C003429T2MELO3C003429T2-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C003429T2.cds1MELO3C003429T2.cds1CDS
MELO3C003429T2.cds2MELO3C003429T2.cds2CDS
MELO3C003429T2.cds3MELO3C003429T2.cds3CDS
MELO3C003429T2.cds4MELO3C003429T2.cds4CDS
MELO3C003429T2.cds5MELO3C003429T2.cds5CDS
MELO3C003429T2.cds6MELO3C003429T2.cds6CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 337..384
score: 1.9E-4coord: 510..593
score:
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 337..385
score: 10
IPR002867IBR domainPFAMPF01485IBRcoord: 405..463
score: 2.1E-9coord: 504..543
score: 7.
IPR002867IBR domainSMARTSM00647ibrneu5coord: 492..554
score: 8.6E-5coord: 405..463
score: 3.2
IPR006575RWD domainPFAMPF05773RWDcoord: 143..277
score: 9.0
IPR006575RWD domainSMARTSM00591RWD2001bcoord: 147..282
score: 7.3
IPR006575RWD domainPROFILEPS50908RWDcoord: 147..282
score: 16
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 335..371
score: 4.5E-9coord: 424..434
score: 4.
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 136..277
score: 1.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 141..276
score: 1.37
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 354..363
scor
NoneNo IPR availablePANTHERPTHR11685:SF121F5D14.10 PROTEINcoord: 93..600
score: 2.0E
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 334..418
score: 1.13E-17coord: 405..467
score: 1.34E-7coord: 496..549
score: 6.18