Lsi11G000820.1 (mRNA) Bottle gourd (USVL1VR-Ls)

NameLsi11G000820.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCytochrome P450
Locationchr11 : 866574 .. 868736 (-)
Sequence length1675
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCTCTGGAAGACTTTCTTCTTTCAGTACTAATTCTCCTCCTACCTTTGCTTTTCATTGTTTTCAAATTGTTTAGCTCTCCATCTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAAAAATGCTCACATTTCAATCACTCACCTTGCCCAAACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCGGCTGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCCGGTCGTTACGTTTTTCATATGACTCCCGATCGAGCACAACATCGCGAATGCTCCCTCGTGTTTTCGCCTGAATGCAACGATCATTGGAGGAGTTTAAAGTCCATTTGCAAAGCTAATTTGTTCACAACAAAAGCAATAGAATCACAGGCTAGTTTGAGGAGGAGGAAAATGAATGAAATGGTGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTTGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTAATGGTGATGGATTCAATGGTATCAAACAACCTTTTAACAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGTGACTTCTATCCCTTGTTGAGAAATTTAGATATGCAAGGTCTTCGAAACAAATGTTCCCTCTATAGTAAACACATCCATTCTACTTTGGAAATTCTAATCAATCAAAGAAGAAAACTTTCTTCTTCAAATTCTTCAGCGCCACAAGACTTCTTGGATGTGTTGATTCAAAGTCAATTCACTGATGACCAAATTAACTACTTAGTTGTTGTAAGCAAACAATAAACCCTCTTTTTTTTTTTTCTTTTTATTTATTTTCATGTAGTAGTAAGGGTGTTCATCAGTTAGTCGGTGTTGGTTTTAGCCTAAAACCGTTGCTGACCACTGATATGTCAGTTTCAATCGGTCGGTTTCTAACGAGTTGTTAGACTACCTACACAGTCTCAAATTATCATTTATGCTCATTTAATTGCATAGATTTCAATTTTTTTTTTTTTTTTTAAAAAAACTTTACATTTCACTTGGTTTATTCAAATATATTTTTTACTCCGACCAATCGACTGGTTGCAAAACCAACCCCGACCAACACCAATTCGGCCGGTCAGATCAGTTTTTGGAGTTTCTTTGAACACCCTTATGAAGCAACACTTTGATGCTTTCTAGGCTCCACTTATCTTTATGTAGCTCCTCGGTTCATTGGAAGAGCCGTGCAACTGATCCAAATTCTAAGTCTTTTAGATCTGTTTTGCAAATTGTAGGAACTTTTGTTGGCTGGCACAGACACAAGTACAATGACAGTGGAGTGGACAATGGCAGAACTGTTAAAGAACAAAGACATAATGGAGAAAGTGAGAGAAGAGATGAAAAGAGAAATAAAGCAAAATAATATTGATGAAACAGAGGTTTGTAAGCTTAAATATTTGTGGAAATGTGTGAAAGAAACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGAATAGCACCAATGGATTGTGAAGTTATGGGATACTCAATTCCAAAGGACAGTCTCATTTTTGTCAACGTTTGGGGCCTTGGAAGAGACCCTTCAATTTGGGAAAACTCATTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATATGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGCCTTCCAATGGCAACTGTTCAACTTCCTTTGATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGATGGTGATGGTGAAGATTTTTCAAAGTTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAAGAGCCACTCAAACTCATTCCTAAAAAGAGGTTATGACATTACCAAATTCTTCATCCTCAATTTGCCTTGCTTTATGAACTATATATATTTGTTGTATTTGTTTTGTATCACAATCTGTTACTTTGTGTGCTTTTTAATGATTTTTCAGAATAAGGTAATATGTATAAACATATAAATATTAAACAAATAAATAATTAGACATTCTAAAGTT

mRNA sequence

ATGGCATCTCTGGAAGACTTTCTTCTTTCAGTACTAATTCTCCTCCTACCTTTGCTTTTCATTGTTTTCAAATTGTTTAGCTCTCCATCTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAAAAATGCTCACATTTCAATCACTCACCTTGCCCAAACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCGGCTGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCCGGTCGTTACGTTTTTCATATGACTCCCGATCGAGCACAACATCGCGAATGCTCCCTCGTGTTTTCGCCTGAATGCAACGATCATTGGAGGAGTTTAAAGTCCATTTGCAAAGCTAATTTGTTCACAACAAAAGCAATAGAATCACAGGCTAGTTTGAGGAGGAGGAAAATGAATGAAATGGTGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTTGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTAATGGTGATGGATTCAATGGTATCAAACAACCTTTTAACAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGTGACTTCTATCCCTTGTTGAGAAATTTAGATATGCAAGGTCTTCGAAACAAATGTTCCCTCTATAGTAAACACATCCATTCTACTTTGGAAATTCTAATCAATCAAAGAAGAAAACTTTCTTCTTCAAATTCTTCAGCGCCACAAGACTTCTTGGATGTGTTGATTCAAAGTCAATTCACTGATGACCAAATTAACTACTTAGTTGTTGAACTTTTGTTGGCTGGCACAGACACAAGTACAATGACAGTGGAGTGGACAATGGCAGAACTGTTAAAGAACAAAGACATAATGGAGAAAGTGAGAGAAGAGATGAAAAGAGAAATAAAGCAAAATAATATTGATGAAACAGAGGTTTGTAAGCTTAAATATTTGTGGAAATGTGTGAAAGAAACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGAATAGCACCAATGGATTGTGAAGTTATGGGATACTCAATTCCAAAGGACAGTCTCATTTTTGTCAACGTTTGGGGCCTTGGAAGAGACCCTTCAATTTGGGAAAACTCATTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATATGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGCCTTCCAATGGCAACTGTTCAACTTCCTTTGATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGATGGTGATGGTGAAGATTTTTCAAAGTTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAAGAGCCACTCAAACTCATTCCTAAAAAGAGGTTATGACATTACCAAATTCTTCATCCTCAATTTGCCTTGCTTTATGAACTATATATATTTGTTGTATTTGTTTTGTATCACAATCTGTTACTTTGTGTGCTTTTTAATGATTTTTCAGAATAAGGTAATATGTATAAACATATAAATATTAAACAAATAAATAATTAGACATTCTAAAGTT

Coding sequence (CDS)

ATGGCATCTCTGGAAGACTTTCTTCTTTCAGTACTAATTCTCCTCCTACCTTTGCTTTTCATTGTTTTCAAATTGTTTAGCTCTCCATCTTCAAAACAACCTCCAATTCCACCTGGCCCAAAACCATGGCCAATTCTTGGAAACCTCCTTCAAATAGGCAAAAATGCTCACATTTCAATCACTCACCTTGCCCAAACCTATGGCCCTCTAATCTCGCTTCGGTTCGGAGGTCGACTACTCGTCGTTGCTTCGTCTCCGGCTGCTGCCGCTGCCGTACTCAAGACCCAAGATCGCCTTCTATCCGGTCGTTACGTTTTTCATATGACTCCCGATCGAGCACAACATCGCGAATGCTCCCTCGTGTTTTCGCCTGAATGCAACGATCATTGGAGGAGTTTAAAGTCCATTTGCAAAGCTAATTTGTTCACAACAAAAGCAATAGAATCACAGGCTAGTTTGAGGAGGAGGAAAATGAATGAAATGGTGGAGTTCTTGTTGTCAAAACAAGGGTCAACAGTTGAGATTAGAGATTTTGTGTTGACTACTGTTTTTAACATTCTCTCTAACTTCATCTTCTCAAGGGATTTGTTTGATTTTAATGGTGATGGATTCAATGGTATCAAACAACCTTTTAACAATCTTAAGAATTTGGGGTTGACTCCAAATTTAGGTGACTTCTATCCCTTGTTGAGAAATTTAGATATGCAAGGTCTTCGAAACAAATGTTCCCTCTATAGTAAACACATCCATTCTACTTTGGAAATTCTAATCAATCAAAGAAGAAAACTTTCTTCTTCAAATTCTTCAGCGCCACAAGACTTCTTGGATGTGTTGATTCAAAGTCAATTCACTGATGACCAAATTAACTACTTAGTTGTTGAACTTTTGTTGGCTGGCACAGACACAAGTACAATGACAGTGGAGTGGACAATGGCAGAACTGTTAAAGAACAAAGACATAATGGAGAAAGTGAGAGAAGAGATGAAAAGAGAAATAAAGCAAAATAATATTGATGAAACAGAGGTTTGTAAGCTTAAATATTTGTGGAAATGTGTGAAAGAAACTCTAAGATTACACCCACCTGCCCCATTTCTTCTTCCACGAATAGCACCAATGGATTGTGAAGTTATGGGATACTCAATTCCAAAGGACAGTCTCATTTTTGTCAACGTTTGGGGCCTTGGAAGAGACCCTTCAATTTGGGAAAACTCATTATCATTCAATCCTCAGAGATTTAGTGATTATGATCATATGGAGTTTAAAGGGAATGATTATGGATACCTTCCATTTGGAGGAGGAAGAAGAATTTGCCCTGGCCTTCCAATGGCAACTGTTCAACTTCCTTTGATTTTAGCCACTTTAATTCATAATTTTGAATGGTCACTTCCTGATGGTGATGGTGATGGTGAAGATTTTTCAAAGTTGGATATGGAAGGGATGCTTGGTGTAACTTTGCAGAAGAAAGAGCCACTCAAACTCATTCCTAAAAAGAGGTTATGA

Protein sequence

MASLEDFLLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILSNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQKKEPLKLIPKKRL
BLAST of Lsi11G000820.1 vs. Swiss-Prot
Match: C80B2_COPJA (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.0e-111
Identity = 217/488 (44.47%), Postives = 308/488 (63.11%), Query Frame = 1

Query: 13  ILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPLIS 72
           I ++   F++F  F    S+   +PPGP+P PI+GNLLQ+G   H     LAQ YG L S
Sbjct: 6   IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFS 65

Query: 73  LRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDHWRS 132
           L+ G + +VVASSPAAAA +LKT D++LSGRYVF     + +H E S+V+S ECND+W+ 
Sbjct: 66  LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVK-EHVENSIVWS-ECNDNWKL 125

Query: 133 LKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILSNFIF 192
           L+ +C+  LFT K IESQ+ +R  K  EMV+FL  K+G  V+I + V  T+ NI  N IF
Sbjct: 126 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIF 185

Query: 193 SRDLFDFNG--DGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSKHIH 252
           S+D+FD      G   +K+    L ++G + N  D++P++  LD+ G R   +   + I+
Sbjct: 186 SKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIY 245

Query: 253 STLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMTVEWT 312
               +++ +RR   +  S +  DF+DVL+ +   D +IN L++EL  AGT+TS  T+EW 
Sbjct: 246 DVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWA 305

Query: 313 MAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
           + EL K   ++ K+R E+   +  N + E+++  L YL   VKETLRLHPP P LLPR A
Sbjct: 306 ITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRA 365

Query: 373 PMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYGYLPF 432
              C VM Y+IPK+  I VN W +GRDP  W++ L+F P+RF   D +++KGND+  +PF
Sbjct: 366 LETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSD-VDYKGNDFELIPF 425

Query: 433 GGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQKKEP 492
           GGGRRICPGLP+A+    LI+ATL+ NFEWSLP     G   S+L M+   G+TLQK  P
Sbjct: 426 GGGRRICPGLPLASQFSNLIVATLVQNFEWSLP----QGMSTSELSMDEKFGLTLQKDPP 484

Query: 493 LKLIPKKR 499
           L ++ K R
Sbjct: 486 LLIVLKAR 484

BLAST of Lsi11G000820.1 vs. Swiss-Prot
Match: C80B3_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.0e-107
Identity = 210/491 (42.77%), Postives = 309/491 (62.93%), Query Frame = 1

Query: 11  VLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPL 70
           V +++   L+++F+  SSP      +PPGPKPWPI+GNLLQ+G+  H     LA+TYG L
Sbjct: 3   VAVVITTFLYLIFR-DSSPKG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDL 62

Query: 71  ISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDHW 130
            SL+ G   +VVAS+P AA+ +LKT DR+LSGRYVF     + +H E S+V+S ECN+ W
Sbjct: 63  FSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVK-EHVENSIVWS-ECNETW 122

Query: 131 RSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILSNF 190
           + L+ +C+  LFT K IESQA +R  K  EMVE+L    G+ V+I + V  T+ NI  N 
Sbjct: 123 KKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNL 182

Query: 191 IFSRDLFDFNGDGFNG---IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSK 250
           IFS+++F   GD  +G   +K+    +  LG + N  D++P L   D+ G     +   +
Sbjct: 183 IFSQNIFKL-GDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQ 242

Query: 251 HIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMTV 310
            I+S    ++ +R+     N+S   DF+++L+ S   D QIN L++E+  AGT+TS  T+
Sbjct: 243 GIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTI 302

Query: 311 EWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLLP 370
           EW ++EL KN  +   +R E+   + +  + E+++  + YL   VKETLRLHP  P LLP
Sbjct: 303 EWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLP 362

Query: 371 RIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYGY 430
           R A   C+V+ Y+IPK+  I VN WG+GRDP  W + L F+P+RF +   ++FKGND+  
Sbjct: 363 RRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN-SSIDFKGNDFEL 422

Query: 431 LPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQK 490
           +PFG GRRICPG+P+AT  + LI+++L+ NF+W  P     G D S+L ME   G+TLQK
Sbjct: 423 IPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFP----KGMDPSQLIMEEKFGLTLQK 480

Query: 491 KEPLKLIPKKR 499
           + PL ++PK R
Sbjct: 483 EPPLYIVPKTR 480

BLAST of Lsi11G000820.1 vs. Swiss-Prot
Match: C80B1_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica GN=CYP80B1 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.6e-107
Identity = 205/493 (41.58%), Postives = 307/493 (62.27%), Query Frame = 1

Query: 10  SVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGP 69
           +V ++ + +  I++ LF     K   +PPGPKPWPI+GNLLQ+G+  H     LAQTYG 
Sbjct: 4   TVALIAVIISSILYLLFGGSGHKN--LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGD 63

Query: 70  LISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDH 129
           + +L+ G   +VVAS+ +AA+ +LKT DR+LS RYVF     +  H E S+V+S +C + 
Sbjct: 64  IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTET 123

Query: 130 WRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILSN 189
           W++L+ +C+  LFT K IESQA +R +K  EMVE+L+ KQG  V+I + +  T+ NI  N
Sbjct: 124 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 183

Query: 190 FIFSRDLFDFN--GDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSK 249
            IFS+++F+      G +  K+    +  LG + N  D++P+L   D+ G R + +   K
Sbjct: 184 LIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 243

Query: 250 HIHSTLEILINQRRKLSSSNS-SAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMT 309
            I++    ++ +R+     +   +  DF+DV + S   D QIN L++EL  AGT+TS  T
Sbjct: 244 GIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 303

Query: 310 VEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLL 369
           +EW M EL KN  I  K+R E++  + + ++ E++   L YL   VKETLRLHPP P LL
Sbjct: 304 IEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLL 363

Query: 370 PRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYG 429
           PR A   C ++ Y+IPKD  I VN WG+GRDP  W + L+F+P+RF +   ++F+GND+ 
Sbjct: 364 PRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLN-SSVDFRGNDFS 423

Query: 430 YLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQ 489
            +PFG GRRICPGLP+A   + L++AT + N +W LP    +G     L +E   G+TLQ
Sbjct: 424 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLP----NGMSVDHLIVEEKFGLTLQ 483

Query: 490 KKEPLKLIPKKRL 500
           K+ PL ++PK R+
Sbjct: 484 KEPPLFIVPKSRV 487

BLAST of Lsi11G000820.1 vs. Swiss-Prot
Match: C80B2_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 9.9e-107
Identity = 206/495 (41.62%), Postives = 310/495 (62.63%), Query Frame = 1

Query: 8   LLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTY 67
           +++V ++ + +  I++ LF S   K   +PPGPKPWPI+GNLLQ+G+  H     LAQTY
Sbjct: 3   VVTVALIAVIISSILYLLFGSSGHKN--LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 62

Query: 68  GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECN 127
           G + +L+ G   +VVAS+ +AA+ +LKT DR+LS RYVF     +  H E S+V+S +C 
Sbjct: 63  GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCT 122

Query: 128 DHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNIL 187
           + W++L+ +C+  LFT K IESQA +R +K  EMVE+L+ KQG  V+I + +  T+ NI 
Sbjct: 123 ETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIF 182

Query: 188 SNFIFSRDLFDFN--GDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLY 247
            N IFS+++F+      G +  K+    +  LG + N  D++P+L   D+ G R + +  
Sbjct: 183 GNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAEC 242

Query: 248 SKHIHSTLEILINQRRKLSSSNS-SAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTST 307
            K I++    ++ +R+     +   +  DF+DV + S   D QIN L++EL  AGT+TS 
Sbjct: 243 LKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSA 302

Query: 308 MTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPF 367
            T+EW M EL KN  I  K+R E++  + + ++ E++   L YL   VKETLRLHPP P 
Sbjct: 303 STIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPL 362

Query: 368 LLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGND 427
           LLPR A   C ++ Y+IPKD  I VN WG+GRDP  W + L+F+P+RF +   ++F+GND
Sbjct: 363 LLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLN-SSVDFRGND 422

Query: 428 YGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVT 487
           +  +PFG GRRICPGLP+A   + L++AT + N +W LP    +G     L +E   G+T
Sbjct: 423 FSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLP----NGMSVDHLIVEEKFGLT 482

Query: 488 LQKKEPLKLIPKKRL 500
           LQK+ PL ++PK R+
Sbjct: 483 LQKEPPLFIVPKSRV 488

BLAST of Lsi11G000820.1 vs. Swiss-Prot
Match: C80BX_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.4e-92
Identity = 182/435 (41.84%), Postives = 265/435 (60.92%), Query Frame = 1

Query: 11  VLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPL 70
           V + L+ ++F  F       S    +PPGPKPWPI+GNLLQ+G+  H     LA+TYG L
Sbjct: 4   VTVALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDL 63

Query: 71  ISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDHW 130
            +L+ G + +VVAS+P AA+ VLK  DR L GRYVF     +  H E S+V++ ECN+ W
Sbjct: 64  FTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVK-NHVENSIVWN-ECNETW 123

Query: 131 RSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILSNF 190
           + L+ +C+  LFT K IE+QA +R  K  EMV++L   +G  V+I + +  T+ N+  N 
Sbjct: 124 KKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNL 183

Query: 191 IFSRDLFDFNGDGFNG---IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSK 250
           IFS+D+F   GD  +G   +KQ    +  LG + N GD+ PLL +LD+ G R   +   +
Sbjct: 184 IFSQDIFKL-GDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 243

Query: 251 HIHSTLEILINQRRKLSSS-NSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMT 310
            ++     ++ +RR      N     DF+DVL+ S   D QIN L++++  AGT+T+  T
Sbjct: 244 GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 303

Query: 311 VEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLL 370
           +EW + EL KN  +   +R E+   + +  + E+++  L YL   VKETLRLHPP P L+
Sbjct: 304 IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLI 363

Query: 371 PRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYG 430
           PR A   C+V+ Y+IPK+  I VN WG+GRDP  W + L F+P RF +   ++FKGND+ 
Sbjct: 364 PRRALETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLN-SSIDFKGNDFE 423

Query: 431 YLPFGGGRRICPGLP 442
            +PFG GRRICPG+P
Sbjct: 424 LIPFGAGRRICPGVP 434

BLAST of Lsi11G000820.1 vs. TrEMBL
Match: B9R9F5_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1496790 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 9.9e-138
Identity = 257/497 (51.71%), Postives = 352/497 (70.82%), Query Frame = 1

Query: 7   FLLSVLILLLPLLFIVFKLFSSPSSKQP--PIPPGPKPWPILGNLLQIGKNAHISITHLA 66
           F L+ L+ L  L+ I+ ++ S  SS++   P+PPGPKPWPILGN+LQ+ K  HI I + A
Sbjct: 5   FSLTYLLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFA 64

Query: 67  QTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRE-CSLVFS 126
           + YGPLISLR G ++LVVAS+P++AA VLK  DRLLS R++    P  +   E  ++V++
Sbjct: 65  KVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWN 124

Query: 127 PECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTV 186
           P CNDHW+SL+++C+  LF+ KAIESQA LR +K+ EM+EFL++KQG  V + + V  T+
Sbjct: 125 PACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTI 184

Query: 187 FNILSNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCS 246
           FN +S+ +FS DL  F      G+K    ++  +  +PN+ +FYP+L  LD QGL+ K +
Sbjct: 185 FNTISHLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMT 244

Query: 247 LYSKHIHSTLEILINQRRKLSSSNSSAPQ-DFLDVLIQSQFTDDQINYLVVELLLAGTDT 306
              K +    EI I +RR+    + +AP+ DFLD+ + + F DDQIN+LV+ELL AGTDT
Sbjct: 245 KCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDT 304

Query: 307 STMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPA 366
           ++ +VEW MAELLKNK+ M+KVREE+ REI +N I E+ V +L YL  CVKETLRLHPPA
Sbjct: 305 TSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPA 364

Query: 367 PFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKG 426
           PFL+PR A  +CEVM Y+IPKDS + VNVW +GRDPS+WE+  SF P+RF     ++ KG
Sbjct: 365 PFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLG-SSLDVKG 424

Query: 427 NDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLG 486
           +D+  +PFG GRRICPGLPMAT QL L+LA+LIH F+WSLP+G     D +K+DM    G
Sbjct: 425 HDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGG----DPAKVDMTEKFG 484

Query: 487 VTLQKKEPLKLIPKKRL 500
           +TLQ + PL +IPK +L
Sbjct: 485 ITLQMEHPLLIIPKPKL 495

BLAST of Lsi11G000820.1 vs. TrEMBL
Match: B9RHX8_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1574420 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 2.9e-137
Identity = 262/496 (52.82%), Postives = 351/496 (70.77%), Query Frame = 1

Query: 8   LLSVLILLLPL-LFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQT 67
           L S   LLLPL L IV ++ SS SSK+ P+PPGP+ WPILGN+LQ+GKN HIS+ + A+ 
Sbjct: 8   LTSPSFLLLPLILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHISMANYAKV 67

Query: 68  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTP--DRAQHRECSLVFSP 127
           +GPLISLR G R++VVASSP AAA +LKT DRLLSGRY+   TP  D    R  +LV++P
Sbjct: 68  HGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDR-IALVWNP 127

Query: 128 ECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVF 187
            C+D W+ L+++C++ LF+ KAIESQA+LR +K+ EM++FL SKQG  V I + V TT F
Sbjct: 128 SCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAF 187

Query: 188 NILSNFIFSRDLFDFNGDGFNG-IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCS 247
           N +SN +FS+DL  F   G  G +K   + L  L   PN+ DFYP+L  LD QG++ K  
Sbjct: 188 NTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMK 247

Query: 248 LYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTS 307
              + +    +I I +RR+    ++    DFLDV + + F D QIN+L++EL  AG DT+
Sbjct: 248 NCLERMFGVWDIYIKERRERHVKDARKT-DFLDVFLSNGFDDHQINWLLLELFSAGADTT 307

Query: 308 TMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAP 367
           T TVEW MAELLK   ++EKVREE++ EI ++ I E+ + +LKYL  CVKETLRLHPP P
Sbjct: 308 TTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVP 367

Query: 368 FLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGN 427
           FL+PR AP  CEVM Y+IPK S I VNVW +GRDPS WE+ LS+ P+RF D  +++FKG+
Sbjct: 368 FLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLD-SNLDFKGH 427

Query: 428 DYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGV 487
           ++ +LPFG GRRICPGLPM T QLPLILA+L+H F+WSL +GD    D + LDM     +
Sbjct: 428 NFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGD----DPAMLDMNDKFSI 487

Query: 488 TLQKKEPLKLIPKKRL 500
           TL+K++ L ++PK++L
Sbjct: 488 TLEKEQHLLVVPKRKL 496

BLAST of Lsi11G000820.1 vs. TrEMBL
Match: F6HP58_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0100g00550 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 8.7e-134
Identity = 249/500 (49.80%), Postives = 346/500 (69.20%), Query Frame = 1

Query: 2   ASLEDFLLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISIT 61
           A +  F     +LLLP L I+  +F  P    PP+PPGP PWPI+GNLLQ+GKN H  + 
Sbjct: 8   ADISIFSFLYALLLLPFLVILKHIFLKP----PPLPPGPYPWPIIGNLLQMGKNPHAKLA 67

Query: 62  HLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDR-AQHRECSL 121
           +LA+ +GPL+SLR G +L+VVASSPAAA  VLKT DR LSGRY+    P +  +    S+
Sbjct: 68  NLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSI 127

Query: 122 VFSPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVL 181
           VF+ +CN +W++L++IC+  LF+ KA+ESQ  LR RK+ E+VEFL +K+G  V++ D V 
Sbjct: 128 VFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVF 187

Query: 182 TTVFNILSNFIFSRDLFDFNGDGFNG--IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGL 241
           TT+ NILSN  FS DL DF  +G  G  +K   +     G TPNL D+YP+L  LD+QG+
Sbjct: 188 TTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGI 247

Query: 242 RNKCSLYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLA 301
             K     + I +T E ++ +RR    SN S+ +DFL+ L++  F DDQIN +++EL  A
Sbjct: 248 NRKAKEMFERIPTTWEDILKERRT-QRSNRSSHRDFLEALLEIGFEDDQINQVILELFSA 307

Query: 302 GTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRL 361
           G +TS++TVEW MAEL++N+D M+K+R E+++ + ++ + E+ + +L YL  CVKE LRL
Sbjct: 308 GAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 367

Query: 362 HPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHM 421
           HPPAP LLP +A   C+VMGY+IPKDS IFVN+W + RDP IW++ LSF P+RF D   +
Sbjct: 368 HPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLD-SKL 427

Query: 422 EFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDME 481
           +FKGND+ Y+PFG GRRICPGL +   Q+PLILAT +H F WSLP       D ++LDME
Sbjct: 428 DFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLP----GNMDSAQLDME 487

Query: 482 GMLGVTLQKKEPLKLIPKKR 499
             L +TL+K++PL+L+P+ R
Sbjct: 488 EWLVITLRKEQPLRLVPRVR 497

BLAST of Lsi11G000820.1 vs. TrEMBL
Match: A5AN88_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013219 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 8.7e-134
Identity = 249/500 (49.80%), Postives = 346/500 (69.20%), Query Frame = 1

Query: 2   ASLEDFLLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISIT 61
           A +  F     +LLLP L I+  +F  P    PP+PPGP PWPI+GNLLQ+GKN H  + 
Sbjct: 8   ADISIFSFLYALLLLPFLVILKHIFLKP----PPLPPGPYPWPIIGNLLQMGKNPHAKLA 67

Query: 62  HLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDR-AQHRECSL 121
           +LA+ +GPL+SLR G +L+VVASSPAAA  VLKT DR LSGRY+    P +  +    S+
Sbjct: 68  NLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSI 127

Query: 122 VFSPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVL 181
           VF+ +CN +W++L++IC+  LF+ KA+ESQ  LR RK+ E+VEFL +K+G  V++ D V 
Sbjct: 128 VFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVF 187

Query: 182 TTVFNILSNFIFSRDLFDFNGDGFNG--IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGL 241
           TT+ NILSN  FS DL DF  +G  G  +K   +     G TPNL D+YP+L  LD+QG+
Sbjct: 188 TTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGI 247

Query: 242 RNKCSLYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLA 301
             K     + I +T E ++ +RR    SN S+ +DFL+ L++  F DDQIN +++EL  A
Sbjct: 248 NRKAKEMFERIPTTWEDILKERRT-QRSNRSSHRDFLEALLEIGFEDDQINQVILELFSA 307

Query: 302 GTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRL 361
           G +TS++TVEW MAEL++N+D M+K+R E+++ + ++ + E+ + +L YL  CVKE LRL
Sbjct: 308 GAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 367

Query: 362 HPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHM 421
           HPPAP LLP +A   C+VMGY+IPKDS IFVN+W + RDP IW++ LSF P+RF D   +
Sbjct: 368 HPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLD-SKL 427

Query: 422 EFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDME 481
           +FKGND+ Y+PFG GRRICPGL +   Q+PLILAT +H F WSLP       D ++LDME
Sbjct: 428 DFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLP----GNMDSAQLDME 487

Query: 482 GMLGVTLQKKEPLKLIPKKR 499
             L +TL+K++PL+L+P+ R
Sbjct: 488 EWLVITLRKEQPLRLVPRVR 497

BLAST of Lsi11G000820.1 vs. TrEMBL
Match: B9I4C8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s13580g PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.6e-132
Identity = 252/492 (51.22%), Postives = 333/492 (67.68%), Query Frame = 1

Query: 11  VLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPL 70
           + +LLLPL FI+ K      S+   +PPGPKPWPI+GNLL +G   H+S+  +A+ +GPL
Sbjct: 19  LFLLLLPLTFIILKNIIRSCSESKYLPPGPKPWPIIGNLLHVGNQPHVSLAEIAKIHGPL 78

Query: 71  ISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQH--RECSLVFSPECND 130
           ISLR G +LLVV SS  AAA +LKT DR LS R+V  + P R  H  R  +LV+ PE  D
Sbjct: 79  ISLRLGTQLLVVGSSAKAAAEILKTHDRFLSARHVPQVIP-RESHVLRRVALVWCPESID 138

Query: 131 HWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNILS 190
            W+ L+ +C+  LF+ KAIES A+LR +K+ E+++FL++++G  V I + V +TVFN +S
Sbjct: 139 TWKLLRGLCRTELFSAKAIESSATLREKKVGELMDFLVAREGKVVSIGEVVFSTVFNTIS 198

Query: 191 NFIFSRDLFDFNGDGFN-GIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSK 250
           N +FS DL      G + G+K     L  L  TPN+ DFYP+   LD QGLR K S   +
Sbjct: 199 NLLFSNDLAGLEEKGMSSGLKSHVRKLMLLVATPNIADFYPIFAGLDPQGLRRKLSKLVE 258

Query: 251 HIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMTV 310
              +   I I +RR  S  + S  +DFLDV + + F DDQIN+L  EL  AGTDT+  T+
Sbjct: 259 ETFAIWAINIKERRN-SYVHDSPKRDFLDVFLANGFDDDQINWLAAELFSAGTDTTATTI 318

Query: 311 EWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAPFLLP 370
           EW +AE+LKNK++M+KV EE++REI +N I E++V  L YL  C+KETLRLHPP P L+P
Sbjct: 319 EWAVAEILKNKEVMKKVDEELEREITKNTISESDVSGLPYLNACIKETLRLHPPVPLLVP 378

Query: 371 RIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYGY 430
             A   CEVM Y+IPKDS + VNVW + RDPS WE+ LSF P RF    ++EFKG +Y +
Sbjct: 379 HRATETCEVMKYTIPKDSQVLVNVWAISRDPSTWEDPLSFKPDRFLG-SNLEFKGGNYEF 438

Query: 431 LPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQK 490
           LPFG GRRICPGLPMA   +PLILA+LI  F+WSLP    +GED +KLDM+   GV LQK
Sbjct: 439 LPFGAGRRICPGLPMANKLVPLILASLIRCFDWSLP----NGEDLAKLDMKDKFGVVLQK 498

Query: 491 KEPLKLIPKKRL 500
           ++PL L+PK+RL
Sbjct: 499 EQPLVLVPKRRL 503

BLAST of Lsi11G000820.1 vs. TAIR10
Match: AT2G45570.1 (AT2G45570.1 cytochrome P450, family 76, subfamily C, polypeptide 2)

HSP 1 Score: 340.5 bits (872), Expect = 1.7e-93
Identity = 197/499 (39.48%), Postives = 295/499 (59.12%), Query Frame = 1

Query: 20  FIVFKLFSSPSS--KQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPLISLRFGG 79
           FI+F   + P S  K  P PPGP   PI+GN+  +G+N H S   L++TYGP++SL+FG 
Sbjct: 20  FIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGS 79

Query: 80  RLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVFSPECNDHWRSL 139
              VV +SP AA  VL+T D++LS R     TP  +     H + S+V+ P  +  WR L
Sbjct: 80  LNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPPSSSRWRLL 139

Query: 140 KSICKANLFTTKAIESQASLRRRKMNEMVEFLL--SKQGSTVEIRDFVLTTVFNILSNFI 199
           + +    LF+ + IE+  +LR  K+ E+V F+   S++   V+I      T  NI+SN +
Sbjct: 140 RKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNIL 199

Query: 200 FSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSKHIHS 259
           FS DL +++ +     +     +      P+  +F+P L  LD+QG R      S+ +  
Sbjct: 200 FSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFK 259

Query: 260 TLEILINQR---RKLSSSNSS--APQDFLDVLI------QSQFTDDQINYLVVELLLAGT 319
                I+ +   + L  +NS     +DF+DVL+      +++   + I +L+++L  AGT
Sbjct: 260 VFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGT 319

Query: 320 DTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNI-DETEVCKLKYLWKCVKETLRLH 379
           DT++ TVEW MAELL+N + M K + E+   I Q  + +E+++  L YL   VKET RLH
Sbjct: 320 DTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLH 379

Query: 380 PPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHME 439
           P AP L+PR A  D EV+G+ +PKD+ +FVNVW +GRDP++WENS  F P+RF   D ++
Sbjct: 380 PAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKD-ID 439

Query: 440 FKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEG 499
            +G DY   PFG GRRICPGLP+A   +PL+LA+L+++F+W LP+G G  ED   LDM+ 
Sbjct: 440 LRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVG-SED---LDMDE 499

BLAST of Lsi11G000820.1 vs. TAIR10
Match: AT2G45550.1 (AT2G45550.1 cytochrome P450, family 76, subfamily C, polypeptide 4)

HSP 1 Score: 329.7 bits (844), Expect = 3.1e-90
Identity = 188/485 (38.76%), Postives = 287/485 (59.18%), Query Frame = 1

Query: 30  SSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTYGPLISLRFGGRLLVVASSPAAA 89
           S +   +PPGP   PI+GN+ Q+GKN H S   LA+ YGP++SL+FG    VV +SP AA
Sbjct: 32  SGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAA 91

Query: 90  AAVLKTQDRLLSGRYVFHMTPDRAQ---HRECSLVFSPECNDHWRSLKSICKANLFTTKA 149
             VL+T D++LSGR     + D  +   H E S+++ P  +  WR L+ +    +F+ + 
Sbjct: 92  REVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQR 151

Query: 150 IESQASLRRRKMNEMVEFL--LSKQGSTVEIRDFVLTTVFNILSNFIFSRDLFDFNGDGF 209
            E+  +LR +K+ E+V F+   S++   V+I     TTV NI+SN +FS DL  ++    
Sbjct: 152 TEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKS 211

Query: 210 NGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSKHIHST----LEILINQR 269
           N  +            P+  +++P +  LD+QG R      ++ +       ++  I ++
Sbjct: 212 NEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEK 271

Query: 270 RKLSSSNSSAPQDFLDVLI------QSQFTDDQINYLVVELLLAGTDTSTMTVEWTMAEL 329
              + S   + +DFLD L+      +++  ++ I +L++++  AGTDTS+ T+EW MAEL
Sbjct: 272 SLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAEL 331

Query: 330 LKNKDIMEKVREEMKREIKQNNI-DETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAPMD 389
           L+N   M K + EM R + QN++  E+++  L YL   VKET RLHP AP L+PR A  D
Sbjct: 332 LRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESD 391

Query: 390 CEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGNDYGYLPFGGG 449
            EV+G+ +PKD+ + VNVW +GRDPS+WEN   F P+RF   D ++ KG DY   PFGGG
Sbjct: 392 VEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKD-IDVKGRDYELTPFGGG 451

Query: 450 RRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGVTLQKKEPLKL 499
           RRICPGLP+A   + L+LA+L+++F+W LP+G    ED   LDM+   G+TL +   L  
Sbjct: 452 RRICPGLPLAVKTVSLMLASLLYSFDWKLPNG-VVSED---LDMDETFGITLHRTNTLYA 507

BLAST of Lsi11G000820.1 vs. TAIR10
Match: AT1G33720.1 (AT1G33720.1 cytochrome P450, family 76, subfamily C, polypeptide 6)

HSP 1 Score: 325.1 bits (832), Expect = 7.6e-89
Identity = 200/505 (39.60%), Postives = 289/505 (57.23%), Query Frame = 1

Query: 7   FLLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQT 66
           FL+   IL   L F   +   SP       PPGP   PI+GN+  +GKN H S T L++T
Sbjct: 10  FLIFCFILSCLLFFTTARSRRSPCQLSKS-PPGPPRLPIIGNIHLVGKNPHHSFTDLSKT 69

Query: 67  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPEC 126
           YGP++SL+ G    VV +S  A   VLKT D++LSGRY+   T     H E S+ +    
Sbjct: 70  YGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSN-NHHEFSVGWIHPS 129

Query: 127 NDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLS--KQGSTVEIRDFVLTTVF 186
           +  +R L+ +    LF+ + I++  +LR +K+ E+V FL    ++   V+I      T  
Sbjct: 130 SSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTAL 189

Query: 187 NILSNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSL 246
           NI+SN +FS +L  ++    +  ++     +     P+L +F+P +R LD+QG   K   
Sbjct: 190 NIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRE 249

Query: 247 YSKHIHSTLEILINQRRKLSSSNSS----APQDFLDVLIQSQFTD------DQINYLVVE 306
            S  +        + R    SS S     + +DFLDVLI  Q  D      D+I +L+++
Sbjct: 250 SSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDEIEHLLLD 309

Query: 307 LLLAGTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQN-NIDETEVCKLKYLWKCVK 366
           + +AGTDT++ TVEW MAELL N   M KV++E+   I QN +  E+++ KL YL   VK
Sbjct: 310 MFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLPYLKAVVK 369

Query: 367 ETLRLHPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFS 426
           ET RLHP APFLL R A  + E++G+++ KDS + VNVW +GRDP +WEN   F P+RF 
Sbjct: 370 ETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERFL 429

Query: 427 DYDHMEFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFS 486
             + ++ KG DY   PFG GRRICPGLP+A   + L+LA+L++ FEW LP+G G  ED  
Sbjct: 430 GKE-IDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVG-SED-- 489

Query: 487 KLDMEGMLGVTLQKKEPLKLIPKKR 499
            LDME   G+T+ K  PL  +P K+
Sbjct: 490 -LDMEETFGLTVHKTNPLLAVPLKK 507

BLAST of Lsi11G000820.1 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 322.0 bits (824), Expect = 6.4e-88
Identity = 191/509 (37.52%), Postives = 294/509 (57.76%), Query Frame = 1

Query: 12  LILLLPLLFIVFKLFSSPSSKQ-----PPIPPGPKPWPILGNLLQIGKNAHISITHLAQT 71
           L+LL   +   F +F++  S +       +PPGP   PI+GN+  +GK+ H S   L++T
Sbjct: 9   LLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKT 68

Query: 72  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVF 131
           YGP++SL+ G    VV +SP AA  VL+T D++LS R     +P  A     H++ SLV+
Sbjct: 69  YGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVW 128

Query: 132 SPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLL--SKQGSTVEIRDFVL 191
            P  +  WR L+ +    L + + IE+  +LR  K+ E+V F+   S +  +V+I     
Sbjct: 129 LPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAF 188

Query: 192 TTVFNILSNFIFSRDLFDFNGDG-FNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLR 251
            T  NI+SN +FS DL  +N     NG++    ++ +   TP+  +++P LR LD+QG  
Sbjct: 189 ITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNV 248

Query: 252 NKCSLYSKHIHSTLEILINQRRKLSSSNSS----APQDFLDVLI-----QSQFTDDQINY 311
               + ++ +       I+ +    SS ++    +  DF+D L+     +S+ +   I +
Sbjct: 249 KTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEH 308

Query: 312 LVVELLLAGTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNI-DETEVCKLKYLW 371
           L++++  AGTDTS+ T+EW M ELLKN   M K + E+   I QN I +E+++ KL YL 
Sbjct: 309 LLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQ 368

Query: 372 KCVKETLRLHPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNP 431
             VKET RLH P P L+PR A  D E++G+ + KD+ + VNVW +GRDPS+W+N   F P
Sbjct: 369 AVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEP 428

Query: 432 QRFSDYDHMEFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDG 491
           +RF   D M+ +G DY   PFG GRRICPG+P+A   + L+LA+L+++F+W LP G    
Sbjct: 429 ERFLGKD-MDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKG-VLS 488

Query: 492 EDFSKLDMEGMLGVTLQKKEPLKLIPKKR 499
           ED   LDM+   G+TL K  PL  +P K+
Sbjct: 489 ED---LDMDETFGLTLHKTNPLHAVPVKK 507

BLAST of Lsi11G000820.1 vs. TAIR10
Match: AT3G52970.2 (AT3G52970.2 cytochrome P450, family 76, subfamily G, polypeptide 1)

HSP 1 Score: 308.5 bits (789), Expect = 7.4e-84
Identity = 175/500 (35.00%), Postives = 279/500 (55.80%), Query Frame = 1

Query: 36  IPPGPKPWPILGNLLQI-GKNAHISITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLK 95
           +PPGP PWP++GN+ Q+ G   H S+T L++ +GP+++LR G  L VV SS   A  + K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 96  TQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDHWRSLKSICKANLFTTKAIESQASLR 155
             D  L+GR ++       +  + SL+ + +   +WR L+ +C    F T+ +++ + +R
Sbjct: 96  KHDAALAGRKIYEAMKG-GKSSDGSLI-TAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 156 RRKMNEMVEFLLSKQGS-------------------TVEIRDFVLTTVFNILSNFIFSRD 215
            R +++M+ F+  ++G                    T+++  +     FN++ N +FSRD
Sbjct: 156 SRCVDQMLRFV--EEGGQNGMYYTINNIMTCIICTKTIDVGRYFFLMAFNLIGNLMFSRD 215

Query: 216 LFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSKHIHSTLEI 275
           L D +    +        +      PN+ DF+PLLR LD QG+R K      H+    EI
Sbjct: 216 LLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQF---HVEKAFEI 275

Query: 276 ---LINQRRKLSSSNSS--APQDFLDVLIQ-----------SQFTDDQINYLVVELLLAG 335
               I +R ++     S    +D+LDVL++           S F+   IN +V E+  AG
Sbjct: 276 AGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAG 335

Query: 336 TDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNN--IDETEVCKLKYLWKCVKETLR 395
           TDT+T T+EW +AELL N   + K++ E++   K +N  + E ++  L YL   + ETLR
Sbjct: 336 TDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLR 395

Query: 396 LHPPAPFLLPRIAPMDCEVMG-YSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRF-SDY 455
           LHPP PFL+P  A   C +   Y+IPK++ + VNVW +GRDP  W + + F P+RF SD 
Sbjct: 396 LHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDP 455

Query: 456 DHMEFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKL 496
           D  +FKG DY +LPFG GRR+CP LP+A+  LPL + +++ +F+W+L     +G +  ++
Sbjct: 456 DARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALE----NGLNAEEM 515

BLAST of Lsi11G000820.1 vs. NCBI nr
Match: gi|659073029|ref|XP_008467223.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 676.8 bits (1745), Expect = 2.9e-191
Identity = 341/496 (68.75%), Postives = 399/496 (80.44%), Query Frame = 1

Query: 8   LLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQTY 67
           LL  L+LLLP+ FI+ K   SPSSK P +PPGP PWPILGNLLQIG+NAHISIT  A  Y
Sbjct: 9   LLPSLLLLLPI-FIILKHIKSPSSKLP-LPPGPNPWPILGNLLQIGQNAHISITQFANIY 68

Query: 68  GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECN 127
           GPLISL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC 
Sbjct: 69  GPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECG 128

Query: 128 DHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVFNIL 187
           D W++L+S+CK NLFT KAIESQA LR +KM E+V+FL SKQGS VE++DFV T+VFN L
Sbjct: 129 DQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTL 188

Query: 188 SNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCSLYSK 247
           SN IFS DL DF GDGF GIK PF  + +LGLTPNL DFYPLLR  D+QGL+NK  +Y  
Sbjct: 189 SNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKN 248

Query: 248 HIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTSTMTV 307
            + S   ILI +RR++     SA  DFLDVLIQS F D QIN L++ELL AGTDT+T TV
Sbjct: 249 EVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTV 308

Query: 308 EWTMAELLKNKDIMEKVREEMKREIKQNN-IDETEVCKLKYLWKCVKETLRLHPPAPFLL 367
           EW M+E+LK+KDI+ KVREEMK+  K+N  IDE+ + +L+YLW+CVKETLRLHPPAPFLL
Sbjct: 309 EWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLL 368

Query: 368 PRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFS---DYDHMEFKGN 427
           PR+APMDCEVMGYSIPKDS++FVNVWG+GRDPS+WE+S +FNPQRF      D+++FKG 
Sbjct: 369 PRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGY 428

Query: 428 DYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGV 487
           DY YLPFGGGRRICPGLPMA VQ+PLILATLIHNFEWSLP    + ED SKLD+ G LGV
Sbjct: 429 DYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLP----NDEDLSKLDLNGRLGV 488

Query: 488 TLQKKEPLKLIPKKRL 500
           TLQK E LKLIPK+R+
Sbjct: 489 TLQKYETLKLIPKRRV 498

BLAST of Lsi11G000820.1 vs. NCBI nr
Match: gi|778708326|ref|XP_004143707.2| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 539.3 bits (1388), Expect = 7.3e-150
Identity = 268/395 (67.85%), Postives = 318/395 (80.51%), Query Frame = 1

Query: 108 MTPDRAQHRECSLVFSPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLS 167
           MTPDRA + + SLVFSPEC DHW++L+SICKANLFT KAIESQA LR +KM E+VEFL S
Sbjct: 1   MTPDRALYNQHSLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLES 60

Query: 168 KQGSTVEIRDFVLTTVFNILSNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFY 227
           KQGS V+I+DFV T+VFN LSN IFS DL DF GDGFNGIK  F  + +LGLTPNL DFY
Sbjct: 61  KQGSVVKIKDFVFTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFY 120

Query: 228 PLLRNLDMQGLRNKCSLYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQ 287
           PLLR  D+QGLR K ++Y   + S   ILI +RR++     SA  DFLDVLIQS F+D Q
Sbjct: 121 PLLRRFDLQGLRKKAAIYMNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFSDQQ 180

Query: 288 INYLVVELLLAGTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQN-NIDETEVCKLK 347
           I+ L++ELL A TDT+T TVEW M+E+LK+KDI+ K REE+K+  K++  IDE+ + KL+
Sbjct: 181 IDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQ 240

Query: 348 YLWKCVKETLRLHPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLS 407
           YLW CVKETLRLHPP PFLLPR+APMDCEVMGYSIPKDS+IFVNVWG+GRDPSIWE+S +
Sbjct: 241 YLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQT 300

Query: 408 FNPQRF--SDYDHMEFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPD 467
           FNP+RF     ++++FKG DY YLPFGGGRRICPGLPMA VQ+PLILATL+HNFEWSLP 
Sbjct: 301 FNPERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLP- 360

Query: 468 GDGDGEDFSKLDMEGMLGVTLQKKEPLKLIPKKRL 500
              +GED SKLD+   LGVTLQK E LKLIPK+RL
Sbjct: 361 ---NGEDLSKLDLNERLGVTLQKYETLKLIPKRRL 391

BLAST of Lsi11G000820.1 vs. NCBI nr
Match: gi|255539531|ref|XP_002510830.1| (PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus communis])

HSP 1 Score: 498.4 bits (1282), Expect = 1.4e-137
Identity = 257/497 (51.71%), Postives = 352/497 (70.82%), Query Frame = 1

Query: 7   FLLSVLILLLPLLFIVFKLFSSPSSKQP--PIPPGPKPWPILGNLLQIGKNAHISITHLA 66
           F L+ L+ L  L+ I+ ++ S  SS++   P+PPGPKPWPILGN+LQ+ K  HI I + A
Sbjct: 5   FSLTYLLPLPILVVILTQIISFISSRRQHLPLPPGPKPWPILGNILQVDKKLHICIANFA 64

Query: 67  QTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRE-CSLVFS 126
           + YGPLISLR G ++LVVAS+P++AA VLK  DRLLS R++    P  +   E  ++V++
Sbjct: 65  KVYGPLISLRLGNQVLVVASTPSSAAEVLKNHDRLLSARFILKAIPSESHILERVAIVWN 124

Query: 127 PECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTV 186
           P CNDHW+SL+++C+  LF+ KAIESQA LR +K+ EM+EFL++KQG  V + + V  T+
Sbjct: 125 PACNDHWKSLRALCRTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTI 184

Query: 187 FNILSNFIFSRDLFDFNGDGFNGIKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCS 246
           FN +S+ +FS DL  F      G+K    ++  +  +PN+ +FYP+L  LD QGL+ K +
Sbjct: 185 FNTISHLLFSTDLIGFENQ-IGGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMT 244

Query: 247 LYSKHIHSTLEILINQRRKLSSSNSSAPQ-DFLDVLIQSQFTDDQINYLVVELLLAGTDT 306
              K +    EI I +RR+    + +AP+ DFLD+ + + F DDQIN+LV+ELL AGTDT
Sbjct: 245 KCLKEMFGVWEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDT 304

Query: 307 STMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPA 366
           ++ +VEW MAELLKNK+ M+KVREE+ REI +N I E+ V +L YL  CVKETLRLHPPA
Sbjct: 305 TSTSVEWAMAELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPA 364

Query: 367 PFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKG 426
           PFL+PR A  +CEVM Y+IPKDS + VNVW +GRDPS+WE+  SF P+RF     ++ KG
Sbjct: 365 PFLIPRRATENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLG-SSLDVKG 424

Query: 427 NDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLG 486
           +D+  +PFG GRRICPGLPMAT QL L+LA+LIH F+WSLP+G     D +K+DM    G
Sbjct: 425 HDFELIPFGSGRRICPGLPMATRQLSLVLASLIHCFDWSLPNGG----DPAKVDMTEKFG 484

Query: 487 VTLQKKEPLKLIPKKRL 500
           +TLQ + PL +IPK +L
Sbjct: 485 ITLQMEHPLLIIPKPKL 495

BLAST of Lsi11G000820.1 vs. NCBI nr
Match: gi|255544572|ref|XP_002513347.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Ricinus communis])

HSP 1 Score: 496.9 bits (1278), Expect = 4.1e-137
Identity = 262/496 (52.82%), Postives = 351/496 (70.77%), Query Frame = 1

Query: 8   LLSVLILLLPL-LFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISITHLAQT 67
           L S   LLLPL L IV ++ SS SSK+ P+PPGP+ WPILGN+LQ+GKN HIS+ + A+ 
Sbjct: 8   LTSPSFLLLPLILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHISMANYAKV 67

Query: 68  YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTP--DRAQHRECSLVFSP 127
           +GPLISLR G R++VVASSP AAA +LKT DRLLSGRY+   TP  D    R  +LV++P
Sbjct: 68  HGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDR-IALVWNP 127

Query: 128 ECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVLTTVF 187
            C+D W+ L+++C++ LF+ KAIESQA+LR +K+ EM++FL SKQG  V I + V TT F
Sbjct: 128 SCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAF 187

Query: 188 NILSNFIFSRDLFDFNGDGFNG-IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGLRNKCS 247
           N +SN +FS+DL  F   G  G +K   + L  L   PN+ DFYP+L  LD QG++ K  
Sbjct: 188 NTISNLLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMK 247

Query: 248 LYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLAGTDTS 307
              + +    +I I +RR+    ++    DFLDV + + F D QIN+L++EL  AG DT+
Sbjct: 248 NCLERMFGVWDIYIKERRERHVKDARKT-DFLDVFLSNGFDDHQINWLLLELFSAGADTT 307

Query: 308 TMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRLHPPAP 367
           T TVEW MAELLK   ++EKVREE++ EI ++ I E+ + +LKYL  CVKETLRLHPP P
Sbjct: 308 TTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVP 367

Query: 368 FLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHMEFKGN 427
           FL+PR AP  CEVM Y+IPK S I VNVW +GRDPS WE+ LS+ P+RF D  +++FKG+
Sbjct: 368 FLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLD-SNLDFKGH 427

Query: 428 DYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDMEGMLGV 487
           ++ +LPFG GRRICPGLPM T QLPLILA+L+H F+WSL +GD    D + LDM     +
Sbjct: 428 NFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGD----DPAMLDMNDKFSI 487

Query: 488 TLQKKEPLKLIPKKRL 500
           TL+K++ L ++PK++L
Sbjct: 488 TLEKEQHLLVVPKRKL 496

BLAST of Lsi11G000820.1 vs. NCBI nr
Match: gi|147842082|emb|CAN62647.1| (hypothetical protein VITISV_013219 [Vitis vinifera])

HSP 1 Score: 485.3 bits (1248), Expect = 1.2e-133
Identity = 249/500 (49.80%), Postives = 346/500 (69.20%), Query Frame = 1

Query: 2   ASLEDFLLSVLILLLPLLFIVFKLFSSPSSKQPPIPPGPKPWPILGNLLQIGKNAHISIT 61
           A +  F     +LLLP L I+  +F  P    PP+PPGP PWPI+GNLLQ+GKN H  + 
Sbjct: 8   ADISIFSFLYALLLLPFLVILKHIFLKP----PPLPPGPYPWPIIGNLLQMGKNPHAKLA 67

Query: 62  HLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDR-AQHRECSL 121
           +LA+ +GPL+SLR G +L+VVASSPAAA  VLKT DR LSGRY+    P +  +    S+
Sbjct: 68  NLAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSI 127

Query: 122 VFSPECNDHWRSLKSICKANLFTTKAIESQASLRRRKMNEMVEFLLSKQGSTVEIRDFVL 181
           VF+ +CN +W++L++IC+  LF+ KA+ESQ  LR RK+ E+VEFL +K+G  V++ D V 
Sbjct: 128 VFAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVF 187

Query: 182 TTVFNILSNFIFSRDLFDFNGDGFNG--IKQPFNNLKNLGLTPNLGDFYPLLRNLDMQGL 241
           TT+ NILSN  FS DL DF  +G  G  +K   +     G TPNL D+YP+L  LD+QG+
Sbjct: 188 TTICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGI 247

Query: 242 RNKCSLYSKHIHSTLEILINQRRKLSSSNSSAPQDFLDVLIQSQFTDDQINYLVVELLLA 301
             K     + I +T E ++ +RR    SN S+ +DFL+ L++  F DDQIN +++EL  A
Sbjct: 248 NRKAKEMFERIPTTWEDILKERRT-QRSNRSSHRDFLEALLEIGFEDDQINQVILELFSA 307

Query: 302 GTDTSTMTVEWTMAELLKNKDIMEKVREEMKREIKQNNIDETEVCKLKYLWKCVKETLRL 361
           G +TS++TVEW MAEL++N+D M+K+R E+++ + ++ + E+ + +L YL  CVKE LRL
Sbjct: 308 GAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 367

Query: 362 HPPAPFLLPRIAPMDCEVMGYSIPKDSLIFVNVWGLGRDPSIWENSLSFNPQRFSDYDHM 421
           HPPAP LLP +A   C+VMGY+IPKDS IFVN+W + RDP IW++ LSF P+RF D   +
Sbjct: 368 HPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLD-SKL 427

Query: 422 EFKGNDYGYLPFGGGRRICPGLPMATVQLPLILATLIHNFEWSLPDGDGDGEDFSKLDME 481
           +FKGND+ Y+PFG GRRICPGL +   Q+PLILAT +H F WSLP       D ++LDME
Sbjct: 428 DFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLP----GNMDSAQLDME 487

Query: 482 GMLGVTLQKKEPLKLIPKKR 499
             L +TL+K++PL+L+P+ R
Sbjct: 488 EWLVITLRKEQPLRLVPRVR 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C80B2_COPJA1.0e-11144.47Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=C... [more]
C80B3_PAPSO2.0e-10742.77(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
C80B1_ESCCA7.6e-10741.58(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
C80B2_ESCCA9.9e-10741.62(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=C... [more]
C80BX_PAPSO2.4e-9241.84(S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP8... [more]
Match NameE-valueIdentityDescription
B9R9F5_RICCO9.9e-13851.71(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R... [more]
B9RHX8_RICCO2.9e-13752.82(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R... [more]
F6HP58_VITVI8.7e-13449.80Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0100g00550 PE=3 SV=... [more]
A5AN88_VITVI8.7e-13449.80Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013219 PE=3 SV=1[more]
B9I4C8_POPTR3.6e-13251.22Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s13580g PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G45570.11.7e-9339.48 cytochrome P450, family 76, subfamily C, polypeptide 2[more]
AT2G45550.13.1e-9038.76 cytochrome P450, family 76, subfamily C, polypeptide 4[more]
AT1G33720.17.6e-8939.60 cytochrome P450, family 76, subfamily C, polypeptide 6[more]
AT2G45560.16.4e-8837.52 cytochrome P450, family 76, subfamily C, polypeptide 1[more]
AT3G52970.27.4e-8435.00 cytochrome P450, family 76, subfamily G, polypeptide 1[more]
Match NameE-valueIdentityDescription
gi|659073029|ref|XP_008467223.1|2.9e-19168.75PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... [more]
gi|778708326|ref|XP_004143707.2|7.3e-15067.85PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sat... [more]
gi|255539531|ref|XP_002510830.1|1.4e-13751.71PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus communis][more]
gi|255544572|ref|XP_002513347.1|4.1e-13752.82PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Ricinus com... [more]
gi|147842082|emb|CAN62647.1|1.2e-13349.80hypothetical protein VITISV_013219 [Vitis vinifera][more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR002401Cyt_P450_E_grp-I
IPR001128Cyt_P450
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi11G000820Lsi11G000820gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi11G000820.1.three_prime_UTR.1Lsi11G000820.1.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi11G000820.1.CDS.2Lsi11G000820.1.CDS.2CDS
Lsi11G000820.1.CDS.1Lsi11G000820.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi11G000820.1Lsi11G000820.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 298..315
score: 5.9E-11coord: 350..361
score: 5.9E-11coord: 428..437
score: 5.9E-11coord: 437..448
score: 5.9
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 31..498
score: 3.7E
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..467
score: 3.4E
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 37..498
score: 8.25E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 307..333
score: 2.8E-37coord: 390..414
score: 2.8E-37coord: 427..437
score: 2.8E-37coord: 349..367
score: 2.8E-37coord: 64..83
score: 2.8E-37coord: 287..304
score: 2.8E-37coord: 437..460
score: 2.8E-37coord: 182..200
score: 2.8
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 430..439
scor
NoneNo IPR availableunknownCoilCoilcoord: 311..341
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 6..499
score: 4.8E
NoneNo IPR availablePANTHERPTHR24298:SF122CYTOCHROME P450 MONOOXYGENASE-LIKE PROTEINcoord: 6..499
score: 4.8E