Lsi10G006640.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G006640.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Shikimate kinase
Location	chr10 : 9237032 .. 9239256 (-)
Sequence length	792

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTGTTTGTCTCCCTAGTACAATTGAATTTGTACTTCAGAGTACTCTTAAAGTGTTATATATAGTTTCTAGAATTGATCAAAATGATCTTATCATATTTCATGATTCGAGATTTTCACTTTGTTTAGTTTAGGGGATTGGGTTCTTGCTTAGAATAAAACCTCCTATTAGCCTCGAAGGTAAAAGTTAATATCATGCTAATAATGCTGTATGATAAATAGTAGTTACTTTGTTTTTTTATTCTTCCTTCTTAAAGATAATATTATAATACAGTGAAAAGGTTCATTATCACTTGTGAAAAATAATTTCAAACTGTTCACTGGGCCATTATATTTTGCCATAGACTTGGAACGTGTAACGTTTCGAATTTCTCACTCTTAAAATATTGATAGCGCAAGGCAAGCTCTATTTTATGCATATATGAACTCCAAAACAGAATCTTAGCTGATTAACATACCGGTGAACCTAACTTATCTTGATTCCTGCAGACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGTAAAGAAGATAGACTAGTTGGATACAAGTGCTACATTTTTCCAGCTGAACTTAACTTTCAATATCATCTGACTGAACCTTTATTTTTAACTTTCCAGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCAATTCACGCCCCCTTTTGCATCATGATTCAACTGATGTATACTCAAAGGTATCTGTTTTCAGTGTTTGTTTCCCTTGACTCATAAATATGATTTTCCAGGTTTCTGATGTTGAAGTTCTTTTACATGAAACATACACAGAGTGTATGATTTCAAAGTTTCTGTTTTCTCGTGACAACTTGAGAAATAATAATCCTTGAAGATTAGTTTTAAAATTGACTCTCGAATAATATTTGATTCCTGTTATCTACAGACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAGTAAGACATCTCTCCTTGTTAGTTTATTCATGTATTCTACCAGGGGATGTACAAATAAGAACTTTTAAAATAATGGCAGCTTAATGTCAAAAACTCAATGGTTTTGGTTTTTATTCTCAAATTATAATCTCATAAGACTACCGACCACTAAAAGGATGCCATTTAGTTTAGGATGAGTTTGAAATGAATTTTAGAGGGTAAATTTTAACTTTGACTGCTTTAGCACATACACTTCTTAGTATTTCTTTGATACAGAAGATTGCTTTCATTTAAAGCTCTTTTTTCAAATGCCATTCCAAACAATATGTATTATTTGGTTGTTCCTCTATGCTAAGAATCTAAAATCTCAATATCAAATGACATAAGCTGAATGTTTAACTTGTGCAGAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGTACGGTCTATCTCTCTCCGTCTTTGAATAAATATGATAATTTACACTACAATTAGCTAAAGCCTTTTAGATAACGAGTCTTATTTTATTTCTATAACAATATTTTGCCTCTTGTTTATATTATGGAATCTCAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGATGGCCACAGGAAATGACTCATTTAGGTATAATAGCAGCAATGAAATGAAGTAGATGATGTTTTCATTTCATTTTTCCTTATGGAGTCGTGTATTTTTCTTATGATTTCAGGTACATATGAATAAAGGCAATGGGTGGAACAAATTTGATTTTTTCATTATTTCTGGATAATCTTCTCAATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATATTTATTGATCTCAGTAAGTGTGTTTGATGTAGTGTAGTGTATCTACAATGTATCATCTGAAAAATGTGGATGGTTATAAGTTTCCAAGCATATGGAAAAACTAGCCATTAGTTGTATTTGAAAGAGTTGGTGAATTCATCTAAGTTAAGAACTTGATGATATCATTCAATTAAGGTTATATTATTGAAATACCCCTAAATTAGGTTCTATTGCATTAGATTTGAAAA

mRNA sequence

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTTTCTGATGTTGAAACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGATGGCCACAGGAAATGACTCATTTAGGTACATATGAATAAAGGCAATGGGTGGAACAAATTTGATTTTTTCATTATTTCTGGATAATCTTCTCAATCAAGTATCAAGGCATGTTTCAGTTTCACTCAAGGCTGTCCCATATTTATTGATCTCAGTAAGTGTGTTTGATGTAGTGTAGTGTATCTACAATGTATCATCTGAAAAATGTGGATGGTTATAAGTTTCCAAGCATATGGAAAAACTAGCCATTAGTTGTATTTGAAAGAGTTGGTGAATTCATCTAAGTTAAGAACTTGATGATATCATTCAATTAAGGTTATATTATTGAAATACCCCTAAATTAGGTTCTATTGCATTAGATTTGAAAA

Coding sequence (CDS)

ATGGATCTTGATCGGGCTGCTCTTTTTCTCTTCAATAATGAGGATATTTCAACTACGGAGGCATTGAGGAAGTTATCTTTGATGCGTCAGTTTGTTATTTCCACTGGTGGAGGTGCCGTTACGAGAACGATAAACTGGAAATATATGCATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGGAAGCCTTGGTTAAGAGAATTTCAGCTGTTTCTGATGTTGAAACCCTCGTCCGTTTATCTACTCTCCTGGAAGAGAGGGGTGAAGCATATGCCAACGCTGAAGTCAAAGTTTCCTGCGAAAAAATTGCAGCCAAACTGGGTACTAAAGACGTATCTAATGTCACACCTATGGCCATAGCAATCGAGGCACTCGAACAAATCGAGATCTTTTTGAAGAGAGAAGATGGTTATTGTGCATTTTGA

Protein sequence

MDLDRAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSDVETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF

Homology

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BLAST of Lsi10G006640.1 vs. Swiss-Prot
Match: SK2_ARATH (Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana GN=SK2 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 8.6e-32
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 1


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 159 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 218

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 219 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 278

[Close]

BLAST of Lsi10G006640.1 vs. Swiss-Prot
Match: SK1_ARATH (Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana GN=SK1 PE=1 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-31
Identity = 79/148 (53.38%), Postives = 100/148 (67.57%), Query Frame = 1


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F+ + E+      T+AL+KLS   Q V+STGGGAV R INWKYMHKGIS+WLDVPLE
Sbjct: 147 AEIFVHHGENFFRGKETDALKKLSSRYQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLE 206

Query: 66  ALVKRISAV-SDVETLV-------------RLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           AL  RI+AV +D   L+             RLS + +ERGEAY NA  +VS E IAAK G
Sbjct: 207 ALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLSAIWDERGEAYTNANARVSLENIAAKRG 266

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKRED 137
            K+VS++TP  IAIEA EQ+  FL++E+
Sbjct: 267 YKNVSDLTPTEIAIEAFEQVLSFLEKEE 294

[Close]

BLAST of Lsi10G006640.1 vs. Swiss-Prot
Match: SK_SOLLC (Shikimate kinase, chloroplastic OS=Solanum lycopersicum GN=SK PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 6.8e-29
Identity = 79/145 (54.48%), Postives = 92/145 (63.45%), Query Frame = 1


Query: 5   RAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEAL 64
           R   F  +NE    TE L KLSLM + V+STGGGAV R INW++MHKGISVWLDVPLEAL
Sbjct: 155 RGESFFRDNE----TEVLHKLSLMHRLVVSTGGGAVVRPINWRHMHKGISVWLDVPLEAL 214

Query: 65  VKRISA------------VSDV--ETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTK 124
            KRI+               DV   TL RL+TL+E RGE YANA  +VS E IA K   K
Sbjct: 215 AKRITTEGTKSRPLLHEESGDVYDTTLKRLTTLMETRGENYANASARVSLENIALK-REK 274

Query: 125 DVSNVTPMAIAIEALEQIEIFLKRE 136
           DV ++TP  I +E L QIE FLK +
Sbjct: 275 DVCHITPAEITLEVLIQIENFLKTQ 294

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BLAST of Lsi10G006640.1 vs. Swiss-Prot
Match: SK2_ORYSJ (Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica GN=SK2 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-27
Identity = 73/143 (51.05%), Postives = 94/143 (65.73%), Query Frame = 1


Query: 4   DRAALFLFNNEDISTTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEA 63
           + +  F  +NE    +  LR LS MR+ V++TGGGAV R +NWKYM KG+SVWLDVPL+A
Sbjct: 144 EHSEAFFRDNE----SSVLRDLSSMRRLVVATGGGAVIRPVNWKYMKKGLSVWLDVPLDA 203

Query: 64  LVKRISAV------------SDVETLV--RLSTLLEERGEAYANAEVKVSCEKIAAKLGT 123
           L +RI+ V            SD  T    +LS L E+RG+AYANA+ +VS E+IAAK G 
Sbjct: 204 LARRIAQVGTASRPLLDQPSSDPYTAAFSKLSMLAEQRGDAYANADARVSLEEIAAKQGH 263

Query: 124 KDVSNVTPMAIAIEALEQIEIFL 133
            DVS +TP  IAIEAL +IE F+
Sbjct: 264 DDVSKLTPTDIAIEALLKIENFV 282

[Close]

BLAST of Lsi10G006640.1 vs. Swiss-Prot
Match: SK1_ORYSJ (Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=SK1 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-26
Identity = 66/128 (51.56%), Postives = 88/128 (68.75%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAV------- 78
           +  LR LS M++ V++TGGGAV R +NWKYM KG+SVWLDVPL+AL +RI+ V       
Sbjct: 157 SSVLRDLSSMKRLVVATGGGAVIRPVNWKYMKKGLSVWLDVPLDALARRIAKVGTASRPL 216

Query: 79  -----SDVETLV--RLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 133
                 D  T+   +LS L E+RG+AYANA+V+VS E+IA+K G  DVS +TP  IAIE+
Sbjct: 217 LDQPSGDPYTMAFSKLSMLAEQRGDAYANADVRVSLEEIASKQGHDDVSKLTPTDIAIES 276

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BLAST of Lsi10G006640.1 vs. TrEMBL
Match: A0A0A0LJX8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296130 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.2e-53
Identity = 121/158 (76.58%), Postives = 126/158 (79.75%), Query Frame = 1


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640.1 vs. TrEMBL
Match: Q6PLR3_CUCSA (Shikimate kinase (Fragment) OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.8e-53
Identity = 116/137 (84.67%), Postives = 119/137 (86.86%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLEALVKRISAV       
Sbjct: 21  TEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPL 80

Query: 79  ---------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 138
                     +TLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA
Sbjct: 81  LHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 140

Query: 139 LEQIEIFLKREDGYCAF 142
           LE+IE FLKREDGYCAF
Sbjct: 141 LEEIETFLKREDGYCAF 157

[Close]

BLAST of Lsi10G006640.1 vs. TrEMBL
Match: E7BBT4_CUCSA (Shikimate kinase OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 8.1e-53
Identity = 120/158 (75.95%), Postives = 125/158 (79.11%), Query Frame = 1


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEAL KLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640.1 vs. TrEMBL
Match: V4S1P4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026159mg PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.4e-36
Identity = 89/133 (66.92%), Postives = 98/133 (73.68%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TE L+KLSLMRQ V+STGGGAVTR INW+YM KGISVWLDVPLEAL +RI+AV       
Sbjct: 165 TEVLQKLSLMRQLVVSTGGGAVTRPINWRYMQKGISVWLDVPLEALAQRIAAVGTDSRPL 224

Query: 79  ----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 137
                      E L RLSTL EERGEAYANA  +VS E IA KLG KDVS++TP+ IAIE
Sbjct: 225 LHQCESGDAYTEALNRLSTLWEERGEAYANANARVSLENIAVKLGHKDVSSLTPVTIAIE 284

[Close]

BLAST of Lsi10G006640.1 vs. TrEMBL
Match: A0A067F9P0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025114mg PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.4e-36
Identity = 89/133 (66.92%), Postives = 98/133 (73.68%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TE L+KLSLMRQ V+STGGGAVTR INW+YM KGISVWLDVPLEAL +RI+AV       
Sbjct: 118 TEVLQKLSLMRQLVVSTGGGAVTRPINWRYMQKGISVWLDVPLEALAQRIAAVGTDSRPL 177

Query: 79  ----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIE 137
                      E L RLSTL EERGEAYANA  +VS E IA KLG KDVS++TP+ IAIE
Sbjct: 178 LHQCESGDAYTEALNRLSTLWEERGEAYANANARVSLENIAVKLGHKDVSSLTPVTIAIE 237

[Close]

BLAST of Lsi10G006640.1 vs. TAIR10
Match: AT4G39540.3 (AT4G39540.3 shikimate kinase 2)

HSP 1 Score: 137.9 bits (346), Expect = 4.9e-33
Identity = 76/135 (56.30%), Postives = 91/135 (67.41%), Query Frame = 1


Query: 19  TEALRKLSLM-RQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD---- 78
           TEAL+KLSLM  Q V+STGGGAV R INWKYMHKGIS+WLDVPLEAL  RI+AV      
Sbjct: 167 TEALKKLSLMYHQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRP 226

Query: 79  -----------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAI 138
                         L RLST+ + RGEAY  A  +VS E I  KLG + VS++TP  IAI
Sbjct: 227 LLHDDESGDTYTAALNRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAI 286

[Close]

BLAST of Lsi10G006640.1 vs. TAIR10
Match: AT2G21940.4 (AT2G21940.4 shikimate kinase 1)

HSP 1 Score: 137.5 bits (345), Expect = 6.3e-33
Identity = 79/148 (53.38%), Postives = 100/148 (67.57%), Query Frame = 1


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F+ + E+      T+AL+KLS   Q V+STGGGAV R INWKYMHKGIS+WLDVPLE
Sbjct: 148 AEIFVHHGENFFRGKETDALKKLSSRYQVVVSTGGGAVIRPINWKYMHKGISIWLDVPLE 207

Query: 66  ALVKRISAV-SDVETLV-------------RLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           AL  RI+AV +D   L+             RLS + +ERGEAY NA  +VS E IAAK G
Sbjct: 208 ALAHRIAAVGTDSRPLLHDESGDAYSVAFKRLSAIWDERGEAYTNANARVSLENIAAKRG 267

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKRED 137
            K+VS++TP  IAIEA EQ+  FL++E+
Sbjct: 268 YKNVSDLTPTEIAIEAFEQVLSFLEKEE 295

[Close]

BLAST of Lsi10G006640.1 vs. TAIR10
Match: AT3G26900.1 (AT3G26900.1 shikimate kinase like 1)

HSP 1 Score: 70.9 bits (172), Expect = 7.3e-13
Identity = 44/117 (37.61%), Postives = 62/117 (52.99%), Query Frame = 1


Query: 17  STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVK----RISAVS 76
           S TE L++LS M + V+  G GAV    N   +  GIS+W+DVPL+   K       +  
Sbjct: 148 SETEVLKQLSSMGRLVVCAGDGAVQSLRNLALLRHGISIWIDVPLDITAKGDDDSFHSEP 207

Query: 77  DVETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIE 130
             E    L    E+  + Y  A+V +S EKIA KL  +D+  VT   IA+E L++IE
Sbjct: 208 SPELFDTLKASYEKSRKGYETADVSISLEKIATKLEFEDLEAVTSEDIALEILKEIE 264

[Close]

BLAST of Lsi10G006640.1 vs. NCBI nr
Match: gi|778670141|ref|XP_011649381.1| (PREDICTED: shikimate kinase, chloroplastic [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-53
Identity = 121/158 (76.58%), Postives = 126/158 (79.75%), Query Frame = 1


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

[Close]

BLAST of Lsi10G006640.1 vs. NCBI nr
Match: gi|659116092|ref|XP_008457898.1| (PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-53
Identity = 121/153 (79.08%), Postives = 124/153 (81.05%), Query Frame = 1


Query: 6   AALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLE 65
           A +F    ED      TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLE
Sbjct: 146 AEIFKVYGEDFFRERETEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLE 205

Query: 66  ALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLG 125
           ALVKRISAV                 +TLVRLSTLLEERGEAYANAEV VSCEKIAAKLG
Sbjct: 206 ALVKRISAVGTNSRPLLHHDSTDAYSKTLVRLSTLLEERGEAYANAEVTVSCEKIAAKLG 265

Query: 126 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF
Sbjct: 266 TKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

[Close]

BLAST of Lsi10G006640.1 vs. NCBI nr
Match: gi|46849856|gb|AAT02351.1| (shikimate kinase, partial [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 4.0e-53
Identity = 116/137 (84.67%), Postives = 119/137 (86.86%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TEALRKLSLMRQFVISTGGGAVTR+INWKYMHKGISVWLDVPLEALVKRISAV       
Sbjct: 21  TEALRKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPL 80

Query: 79  ---------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 138
                     +TLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA
Sbjct: 81  LHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 140

Query: 139 LEQIEIFLKREDGYCAF 142
           LE+IE FLKREDGYCAF
Sbjct: 141 LEEIETFLKREDGYCAF 157

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BLAST of Lsi10G006640.1 vs. NCBI nr
Match: gi|793418105|ref|NP_001292691.1| (shikimate kinase, chloroplastic [Cucumis sativus])

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-52
Identity = 120/158 (75.95%), Postives = 125/158 (79.11%), Query Frame = 1


Query: 1   MDLDRAALFLFNNEDI---STTEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWL 60
           M +  A +F    ED      TEAL KLSLMRQFVISTGGGAVTR+INWKYMHKGISVWL
Sbjct: 145 MGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVISTGGGAVTRSINWKYMHKGISVWL 204

Query: 61  DVPLEALVKRISAVSD--------------VETLVRLSTLLEERGEAYANAEVKVSCEKI 120
           DVPLEALVKRISAV                 +TLVRLSTLLEERGEAYANAEVKVSCEKI
Sbjct: 205 DVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVRLSTLLEERGEAYANAEVKVSCEKI 264

Query: 121 AAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 142
           AAKLGTKDVSNVTPMAIAIEALE+IE FLKREDGYCAF
Sbjct: 265 AAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYCAF 302

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BLAST of Lsi10G006640.1 vs. NCBI nr
Match: gi|1021530104|ref|XP_016162009.1| (PREDICTED: shikimate kinase 1, chloroplastic-like [Arachis ipaensis])

HSP 1 Score: 161.8 bits (408), Expect = 8.9e-37
Identity = 86/135 (63.70%), Postives = 99/135 (73.33%), Query Frame = 1


Query: 19  TEALRKLSLMRQFVISTGGGAVTRTINWKYMHKGISVWLDVPLEALVKRISAVSD----- 78
           TE LRKLS+  + VISTGGGAV R INWKYMHKG+S+WLDVP+EAL +RI+AV       
Sbjct: 167 TEVLRKLSMTHRHVISTGGGAVVRPINWKYMHKGVSIWLDVPVEALAQRITAVGTNSRPL 226

Query: 79  ---------VETLVRLSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEA 138
                     +T +RLS+L EER +AYANA  KVS E IAAKLG KDVS++TP AIAIEA
Sbjct: 227 LHSEVGDAYTKTFMRLSSLFEERSQAYANANAKVSLENIAAKLGQKDVSDLTPTAIAIEA 286

Query: 139 LEQIEIFLKREDGYC 140
           LEQI  FLK EDGYC
Sbjct: 287 LEQILGFLKSEDGYC 301

The following BLAST results are available for this feature:

BLAST of Lsi10G006640.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SK2_ARATH	8.6e-32	56.30	Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana GN=SK2 PE=1 SV=1	[more]
SK1_ARATH	1.1e-31	53.38	Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana GN=SK1 PE=1 SV=2	[more]
SK_SOLLC	6.8e-29	54.48	Shikimate kinase, chloroplastic OS=Solanum lycopersicum GN=SK PE=1 SV=1	[more]
SK2_ORYSJ	2.9e-27	51.05	Shikimate kinase 2, chloroplastic OS=Oryza sativa subsp. japonica GN=SK2 PE=1 SV...	[more]
SK1_ORYSJ	1.9e-26	51.56	Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica GN=SK1 PE=1 SV...	[more]

BLAST of Lsi10G006640.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJX8_CUCSA	1.2e-53	76.58	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G296130 PE=3 SV=1	[more]
Q6PLR3_CUCSA	2.8e-53	84.67	Shikimate kinase (Fragment) OS=Cucumis sativus PE=2 SV=1	[more]
E7BBT4_CUCSA	8.1e-53	75.95	Shikimate kinase OS=Cucumis sativus PE=2 SV=1	[more]
V4S1P4_9ROSI	1.4e-36	66.92	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026159mg PE=3 SV=1	[more]
A0A067F9P0_CITSI	1.4e-36	66.92	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025114mg PE=3 SV=1	[more]

BLAST of Lsi10G006640.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G39540.3	4.9e-33	56.30	shikimate kinase 2	[more]
AT2G21940.4	6.3e-33	53.38	shikimate kinase 1	[more]
AT3G26900.1	7.3e-13	37.61	shikimate kinase like 1	[more]

BLAST of Lsi10G006640.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778670141\|ref\|XP_011649381.1\|	1.8e-53	76.58	PREDICTED: shikimate kinase, chloroplastic [Cucumis sativus]	[more]
gi\|659116092\|ref\|XP_008457898.1\|	1.8e-53	79.08	PREDICTED: shikimate kinase, chloroplastic-like [Cucumis melo]	[more]
gi\|46849856\|gb\|AAT02351.1\|	4.0e-53	84.67	shikimate kinase, partial [Cucumis sativus]	[more]
gi\|793418105\|ref\|NP_001292691.1\|	1.2e-52	75.95	shikimate kinase, chloroplastic [Cucumis sativus]	[more]
gi\|1021530104\|ref\|XP_016162009.1\|	8.9e-37	63.70	PREDICTED: shikimate kinase 1, chloroplastic-like [Arachis ipaensis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR027417	P-loop_NTPase

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016301	kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi10G006640	Lsi10G006640	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi10G006640.1	Lsi10G006640.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G006640.1.three_prime_UTR.2	Lsi10G006640.1.three_prime_UTR.2	three_prime_UTR
Lsi10G006640.1.three_prime_UTR.1	Lsi10G006640.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G006640.1.CDS.7	Lsi10G006640.1.CDS.7	CDS
Lsi10G006640.1.CDS.6	Lsi10G006640.1.CDS.6	CDS
Lsi10G006640.1.CDS.5	Lsi10G006640.1.CDS.5	CDS
Lsi10G006640.1.CDS.4	Lsi10G006640.1.CDS.4	CDS
Lsi10G006640.1.CDS.3	Lsi10G006640.1.CDS.3	CDS
Lsi10G006640.1.CDS.2	Lsi10G006640.1.CDS.2	CDS
Lsi10G006640.1.CDS.1	Lsi10G006640.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	G3DSA:3.40.50.300		coord: 18..126 score: 1.8
None	No IPR available	PRINTS	PR01100	SHIKIMTKNASE	coord: 32..41 score: 4.1E-8coord: 52..69 score: 4.
None	No IPR available	PANTHER	PTHR21087	SHIKIMATE KINASE	coord: 19..137 score: 4.8
None	No IPR available	PANTHER	PTHR21087:SF8	SHIKIMATE KINASE 1, CHLOROPLASTIC-RELATED	coord: 19..137 score: 4.8