Lsi10G002420.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi10G002420.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cleavage and polyadenylation specificity factor subunit 3-II
Location	chr10 : 3996041 .. 3996883 (-)
Sequence length	843

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTGTGAAATTCCTTAGTCCCAAGCATGTGATACTTGTACATGGAGAGAAACCTAAAATGGCTGCTTTAAAGGAGAGGATTCATTCTGAACTGGGAATCCCATGTCATGATCCTGCAAATAATGAAACCGTATCCATTTCTTCAACTCTTTCTGTCAAAGCAGAAGCCTCAAGCATGTTTATTCAGAGTTGTTCCACTCCTAATTTCAAGTTCTTGAAAAGAAACTTGAATGATAAGATTGATTCCAATTTAAAAGATTTAAGTTGCAAAGCAGAAGGAACTTCAAGCATGTTTATACGCAAATGCTCAAATCCCCATTTCAAGCATTTGAATAAAAATTTGGACGCCAAGTTTGATTCTAGTTTAAGTTGTGGTCCAGAGCTACAGGTAAGTGATGATAGAGTGAATGAAGGGGTCATGGTGATGGAAAATGGTAAAAAAACAAAGGTGGTACACCAGGATGAACTATTGCTACTCTTGGGAGAACAAGAGCATGAGGTTAGGTTTGCTAATTGTAGACCCATATATTTTGGAAACTTAGATGTGACCCATGTTGTGGATTGCTTATCTAGAAAATCTGTATGGCTTTCCCAGCTATTTTCGAAGCTTTCAAGTGAACTTTCAGACAGGAATGTTCAAAATCTTGGGGAGTATCTTCAAGTTGAATCATTTACACTGTCCATTTGCTCAAAAGAAAATTGCCCTTACAGAACTACAAATAGAATTGAAAATGAATCTGCTATGGTATTCTGTTGCTGTAGTTGGCTAGTTGCAGATGAAACCCTTGCATGGAAAATCATTTCCATCTTGGAGAAGCATAATCTCAGTTCAACATAA

mRNA sequence

ATGGATCTTGTGAAATTCCTTAGTCCCAAGCATGTGATACTTGTACATGGAGAGAAACCTAAAATGGCTGCTTTAAAGGAGAGGATTCATTCTGAACTGGGAATCCCATGTCATGATCCTGCAAATAATGAAACCGTATCCATTTCTTCAACTCTTTCTGTCAAAGCAGAAGCCTCAAGCATGTTTATTCAGAGTTGTTCCACTCCTAATTTCAAGTTCTTGAAAAGAAACTTGAATGATAAGATTGATTCCAATTTAAAAGATTTAAGTTGCAAAGCAGAAGGAACTTCAAGCATGTTTATACGCAAATGCTCAAATCCCCATTTCAAGCATTTGAATAAAAATTTGGACGCCAAGTTTGATTCTAGTTTAAGTTGTGGTCCAGAGCTACAGGTAAGTGATGATAGAGTGAATGAAGGGGTCATGGTGATGGAAAATGGTAAAAAAACAAAGGTGGTACACCAGGATGAACTATTGCTACTCTTGGGAGAACAAGAGCATGAGGTTAGGTTTGCTAATTGTAGACCCATATATTTTGGAAACTTAGATGTGACCCATGTTGTGGATTGCTTATCTAGAAAATCTGTATGGCTTTCCCAGCTATTTTCGAAGCTTTCAAGTGAACTTTCAGACAGGAATGTTCAAAATCTTGGGGAGTATCTTCAAGTTGAATCATTTACACTGTCCATTTGCTCAAAAGAAAATTGCCCTTACAGAACTACAAATAGAATTGAAAATGAATCTGCTATGGTATTCTGTTGCTGTAGTTGGCTAGTTGCAGATGAAACCCTTGCATGGAAAATCATTTCCATCTTGGAGAAGCATAATCTCAGTTCAACATAA

Coding sequence (CDS)

ATGGATCTTGTGAAATTCCTTAGTCCCAAGCATGTGATACTTGTACATGGAGAGAAACCTAAAATGGCTGCTTTAAAGGAGAGGATTCATTCTGAACTGGGAATCCCATGTCATGATCCTGCAAATAATGAAACCGTATCCATTTCTTCAACTCTTTCTGTCAAAGCAGAAGCCTCAAGCATGTTTATTCAGAGTTGTTCCACTCCTAATTTCAAGTTCTTGAAAAGAAACTTGAATGATAAGATTGATTCCAATTTAAAAGATTTAAGTTGCAAAGCAGAAGGAACTTCAAGCATGTTTATACGCAAATGCTCAAATCCCCATTTCAAGCATTTGAATAAAAATTTGGACGCCAAGTTTGATTCTAGTTTAAGTTGTGGTCCAGAGCTACAGGTAAGTGATGATAGAGTGAATGAAGGGGTCATGGTGATGGAAAATGGTAAAAAAACAAAGGTGGTACACCAGGATGAACTATTGCTACTCTTGGGAGAACAAGAGCATGAGGTTAGGTTTGCTAATTGTAGACCCATATATTTTGGAAACTTAGATGTGACCCATGTTGTGGATTGCTTATCTAGAAAATCTGTATGGCTTTCCCAGCTATTTTCGAAGCTTTCAAGTGAACTTTCAGACAGGAATGTTCAAAATCTTGGGGAGTATCTTCAAGTTGAATCATTTACACTGTCCATTTGCTCAAAAGAAAATTGCCCTTACAGAACTACAAATAGAATTGAAAATGAATCTGCTATGGTATTCTGTTGCTGTAGTTGGCTAGTTGCAGATGAAACCCTTGCATGGAAAATCATTTCCATCTTGGAGAAGCATAATCTCAGTTCAACATAA

Protein sequence

MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASSMFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKFDSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFGNLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNLSST

Homology

[Close]

BLAST of Lsi10G002420.1 vs. Swiss-Prot
Match: CPS3B_ARATH (Cleavage and polyadenylation specificity factor subunit 3-II OS=Arabidopsis thaliana GN=CPSF73-II PE=1 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 2.5e-22
Identity = 61/177 (34.46%), Postives = 94/177 (53.11%), Query Frame = 1


Query: 97  SSMFIRKCSNPHFKHLNKNLDAKFDSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQD 156
           S MF++ CSNP+FK  N               +L+V+D R  +GV+V+E  KK K+VHQD
Sbjct: 457 SDMFLKSCSNPNFKFSNST-------------QLRVTDHRTADGVLVIEKSKKAKIVHQD 516

Query: 157 ELLLLLGEQEHEVRFANCRPIYFGNLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQN 216
           E+  +L E+ H V  A+C P+          VD        + QL +K+   +S   +  
Sbjct: 517 EISEVLHEKNHVVSLAHCCPVKVKGESEDDDVDL-------IKQLSAKILKTVSGAQIHE 576

Query: 217 LGEYLQVESFTLSICSKENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILE 274
               LQV SF  S+C K+ C +R+++   + S  VF CC+W +AD  L W+II+ ++
Sbjct: 577 SENCLQVASFKGSLCLKDKCMHRSSS---SSSEAVFLCCNWSIADLELGWEIINAIK 610

[Close]

BLAST of Lsi10G002420.1 vs. Swiss-Prot
Match: INT11_DICDI (Integrator complex subunit 11 homolog OS=Dictyostelium discoideum GN=ints11 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 8.6e-07
Identity = 37/123 (30.08%), Postives = 62/123 (50.41%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           + L+K  +P++VILVHGEK KM  L ++I  E+G+ C+ PAN  T+ I +  S+  + S 
Sbjct: 410 LQLIKMSNPRNVILVHGEKEKMGFLSQKIIKEMGVNCYYPANGVTIIIDTMKSIPIDISL 469

Query: 61  MFIQS-----------------CSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRK 107
             ++                   +  N   L  N N+  +++LK +  K   TS++FI  
Sbjct: 470 NLLKRQILDYSYQYNNNNLNNFNNFNNLNNLNNNNNNNNNNSLKLIDIK-NNTSTLFINN 529

[Close]

BLAST of Lsi10G002420.1 vs. Swiss-Prot
Match: INT11_DANRE (Integrator complex subunit 11 OS=Danio rerio GN=cpsf3l PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.5e-06
Identity = 25/59 (42.37%), Postives = 36/59 (61.02%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEAS 60
           M L++   P++++LVHGE  KM  LK++I  E  I C  PAN ET +I +  SV  + S
Sbjct: 399 MQLIRMAEPRNMLLVHGEAKKMEFLKDKIEQEFSISCFMPANGETTTIVTNPSVPVDIS 457

[Close]

BLAST of Lsi10G002420.1 vs. Swiss-Prot
Match: INT11_CHICK (Integrator complex subunit 11 OS=Gallus gallus GN=CPSF3L PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.5e-06
Identity = 24/59 (40.68%), Postives = 37/59 (62.71%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEAS 60
           M L++   P++V+LVHGE  KM  LK++I  E  + C+ PAN ET +I +  S+  + S
Sbjct: 399 MQLIRQAEPRNVLLVHGEAKKMEFLKQKIEQEFHVNCYMPANGETTTIFTNPSIPVDIS 457

[Close]

BLAST of Lsi10G002420.1 vs. Swiss-Prot
Match: INT11_PONAB (Integrator complex subunit 11 OS=Pongo abelii GN=CPSF3L PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 9.5e-06
Identity = 25/54 (46.30%), Postives = 36/54 (66.67%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSV 55
           M LV    P+ V+LVHGE  KM  LK++I  EL + C+ PAN ETV++ ++ S+
Sbjct: 399 MQLVGQAEPESVLLVHGEAKKMEFLKQKIEQELRVSCYMPANGETVTLPTSPSI 452

[Close]

BLAST of Lsi10G002420.1 vs. TrEMBL
Match: Q6E435_CUCME (ACT11D09.9 OS=Cucumis melo GN=ACT11D09.9 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 4.3e-138
Identity = 249/281 (88.61%), Postives = 263/281 (93.59%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKHVILVHGEKPKMA LKERIHSELGIPCHDPANNETVSISSTLS+KAEASS
Sbjct: 428 MDLVKFLSPKHVILVHGEKPKMAVLKERIHSELGIPCHDPANNETVSISSTLSIKAEASS 487

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
           MFIQSCSTPNFKFLKRNL DKID +LKDLS KA  TS+M IR+CSNPHFKHLN+NLDAKF
Sbjct: 488 MFIQSCSTPNFKFLKRNLIDKIDPDLKDLSYKAVRTSNMLIRECSNPHFKHLNRNLDAKF 547

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
           DSSLS GPELQVSDDRVNEG++VMENGKKTK +HQDELLLLLGEQEHEVRFA+CRPIYFG
Sbjct: 548 DSSLSGGPELQVSDDRVNEGILVMENGKKTKALHQDELLLLLGEQEHEVRFAHCRPIYFG 607

Query: 181 NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRT 240
           +LD  HV+D LSRKS+WLSQL  KLS+ELSDRNVQNLGEYLQVES TLSICSKENCPYRT
Sbjct: 608 SLDEIHVMDSLSRKSLWLSQLSFKLSTELSDRNVQNLGEYLQVESITLSICSKENCPYRT 667

Query: 241 TNRIENES-AMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           TNRIENES AMVFCCCSWLVADE LAWKIISILEKH+L ST
Sbjct: 668 TNRIENESTAMVFCCCSWLVADEILAWKIISILEKHDLGST 708

[Close]

BLAST of Lsi10G002420.1 vs. TrEMBL
Match: A0A0A0LQW3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G435430 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-134
Identity = 244/281 (86.83%), Postives = 260/281 (92.53%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKHVILVHGEKPKMA LKERIHSELGIPCHDPANNETVSISSTLSVKAEASS
Sbjct: 399 MDLVKFLSPKHVILVHGEKPKMAVLKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 458

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
           MFIQSCSTPNFKFLKRNL   ID +LKDLS KAE TS+M IR+CSNPHFKHL +NLDAKF
Sbjct: 459 MFIQSCSTPNFKFLKRNL---IDPDLKDLSYKAERTSNMLIRECSNPHFKHLKRNLDAKF 518

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
           DSSLSCGP LQVSDDRVNEG++VMENGKKTK +HQDELLLLLG+QEHEVRFA+CRPIYFG
Sbjct: 519 DSSLSCGPALQVSDDRVNEGILVMENGKKTKALHQDELLLLLGQQEHEVRFAHCRPIYFG 578

Query: 181 NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRT 240
           +LD  HV+D LSRKS+WLSQL  KLS+ELSDRNVQNLGEYLQVES TLSICSKENCPYRT
Sbjct: 579 SLDEIHVMDSLSRKSLWLSQLSFKLSTELSDRNVQNLGEYLQVESITLSICSKENCPYRT 638

Query: 241 TNRIENES-AMVFCCCSWLVADETLAWKIISILEKHNLSST 281
            +RI+NES AMVFCCCSWLVADE LAWKIISILEKH+L ST
Sbjct: 639 IDRIKNESTAMVFCCCSWLVADEILAWKIISILEKHDLGST 676

[Close]

BLAST of Lsi10G002420.1 vs. TrEMBL
Match: V4W5P9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014563mg PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.5e-61
Identity = 140/288 (48.61%), Postives = 177/288 (61.46%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSP+HVILVHGEKPKMA LKERI SELGI C+DPANNE++ I ST  VKA AS 
Sbjct: 394 MDLVKFLSPQHVILVHGEKPKMATLKERIQSELGIKCYDPANNESMCIPSTHYVKAGASD 453

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
            FI+SC  PNF+FLK    +K  S  K   C  EGT  ++I+                  
Sbjct: 454 AFIRSCMNPNFQFLKSGSEEKSVSGSK---C-TEGTLPLWIK------------------ 513

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
                        D+RV EG++V+E  +K KVVHQDELLL+LGE+ HEV+FA C P+   
Sbjct: 514 -------------DERVAEGILVLEKSEKAKVVHQDELLLMLGEKRHEVQFAYCCPVNVD 573

Query: 181 NLD------VTHVVDCL--SRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICS 240
            L+      +T     L    KS  +  L +KLS +LS+ N+Q+ GE+LQVESF LS+C 
Sbjct: 574 ELEKFTTTSLTPTARMLRDPNKSSLIRLLVAKLSRKLSEGNIQDFGEHLQVESFHLSVCL 633

Query: 241 KENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           K+ CPYR TN +E++    F CC+W  AD+ LA KIIS +E  +L  T
Sbjct: 634 KDTCPYRITNGLEDKPRTAFVCCTWSAADDKLARKIISAMENRDLVET 646

[Close]

BLAST of Lsi10G002420.1 vs. TrEMBL
Match: A0A067FBD9_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g043917mg PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.6e-60
Identity = 139/288 (48.26%), Postives = 177/288 (61.46%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSP+HVILVHGEKPKMA LKERI SELGI C+DPANNE++ I ST  VKA AS 
Sbjct: 171 MDLVKFLSPQHVILVHGEKPKMATLKERIQSELGIKCYDPANNESMCIPSTHYVKAGASD 230

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
            FI+SC  PNF++LK    +K  S  K   C  EGT  ++I+                  
Sbjct: 231 AFIRSCMNPNFQYLKSGSEEKSVSGSK---C-TEGTLPLWIK------------------ 290

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
                        D+RV EG++V+E  +K KVVHQDELLL+LGE+ HEV+FA C P+   
Sbjct: 291 -------------DERVAEGILVLEKSEKAKVVHQDELLLMLGEKRHEVQFAYCCPVNVD 350

Query: 181 NLD------VTHVVDCL--SRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICS 240
            L+      +T     L    KS  +  L +KLS +LS+ N+Q+ GE+LQVESF LS+C 
Sbjct: 351 ELEKFTTTSLTPTARMLRDPNKSSLIRLLVAKLSRKLSEGNIQDFGEHLQVESFHLSVCL 410

Query: 241 KENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           K+ CPYR TN +E++    F CC+W  AD+ LA KIIS +E  +L  T
Sbjct: 411 KDTCPYRITNGLEDKPRTAFFCCTWSAADDKLARKIISAMENRDLVET 423

[Close]

BLAST of Lsi10G002420.1 vs. TrEMBL
Match: A0A0D2W0M5_GOSRA (Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_012G1826002 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-54
Identity = 131/283 (46.29%), Postives = 171/283 (60.42%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKH ILVHGEKPKMA LKERI SELGI C+ PAN      + T+++      
Sbjct: 176 MDLVKFLSPKHAILVHGEKPKMATLKERIQSELGIQCYCPAN------NETVTI------ 235

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAK- 120
                   P+  ++K +               A GT   FIR C NP+FK    +   K 
Sbjct: 236 --------PSTHYVKAD---------------ASGT---FIRSCLNPNFKFSTCSSTDKS 295

Query: 121 -FDSSLSCG-PELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPI 180
             DS+ S     L VSDDR  EG++V+E GKK KV+HQDE L +LGE++HE++FA C P+
Sbjct: 296 YLDSNKSKAVSRLSVSDDRATEGILVVEKGKKAKVIHQDEFLHMLGEKKHEIQFAYCFPM 355

Query: 181 YFG---NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKE 240
           +     +L  T  + C   K   +S+L   LS ELS+ N+ +LGE LQVESF +S+CS +
Sbjct: 356 HMARTEDLSSTSHMLCSFDKCTLISRLSMILSDELSEGNILDLGEELQVESFHVSVCSSD 415

Query: 241 NCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNL 278
           NCP+R ++ ++N S  VF CC W VADE LAWKIISI++   L
Sbjct: 416 NCPHRLSSSLQNGSESVFFCCHWSVADEKLAWKIISIMKNSTL 420

[Close]

BLAST of Lsi10G002420.1 vs. TAIR10
Match: AT2G01730.1 (AT2G01730.1 cleavage and polyadenylation specificity factor 73 kDa subunit-II)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-23
Identity = 61/177 (34.46%), Postives = 94/177 (53.11%), Query Frame = 1


Query: 97  SSMFIRKCSNPHFKHLNKNLDAKFDSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQD 156
           S MF++ CSNP+FK  N               +L+V+D R  +GV+V+E  KK K+VHQD
Sbjct: 457 SDMFLKSCSNPNFKFSNST-------------QLRVTDHRTADGVLVIEKSKKAKIVHQD 516

Query: 157 ELLLLLGEQEHEVRFANCRPIYFGNLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQN 216
           E+  +L E+ H V  A+C P+          VD        + QL +K+   +S   +  
Sbjct: 517 EISEVLHEKNHVVSLAHCCPVKVKGESEDDDVDL-------IKQLSAKILKTVSGAQIHE 576

Query: 217 LGEYLQVESFTLSICSKENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILE 274
               LQV SF  S+C K+ C +R+++   + S  VF CC+W +AD  L W+II+ ++
Sbjct: 577 SENCLQVASFKGSLCLKDKCMHRSSS---SSSEAVFLCCNWSIADLELGWEIINAIK 610

[Close]

BLAST of Lsi10G002420.1 vs. NCBI nr
Match: gi|659118488|ref|XP_008459147.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 3-II isoform X1 [Cucumis melo])

HSP 1 Score: 503.4 bits (1295), Expect = 2.5e-139
Identity = 250/281 (88.97%), Postives = 264/281 (93.95%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKHVILVHGEKPKMA LKERIHSELGIPCHDPANNETVSISSTLS+KAEASS
Sbjct: 399 MDLVKFLSPKHVILVHGEKPKMAVLKERIHSELGIPCHDPANNETVSISSTLSIKAEASS 458

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
           MFIQSCSTPNFKFLKRNL DKID +LKDLS KA  TS+M IR+CSNPHFKHLN+NLDAKF
Sbjct: 459 MFIQSCSTPNFKFLKRNLIDKIDPDLKDLSYKAVRTSNMLIRECSNPHFKHLNRNLDAKF 518

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
           DSSLSCGPELQVSDDRVNEG++VMENGKKTK +HQDELLLLLGEQEHEVRFA+CRPIYFG
Sbjct: 519 DSSLSCGPELQVSDDRVNEGILVMENGKKTKALHQDELLLLLGEQEHEVRFAHCRPIYFG 578

Query: 181 NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRT 240
           +LD  HV+D LSRKS+WLSQL  KLS+ELSDRNVQNLGEYLQVES TLSICSKENCPYRT
Sbjct: 579 SLDEIHVMDSLSRKSLWLSQLSFKLSTELSDRNVQNLGEYLQVESITLSICSKENCPYRT 638

Query: 241 TNRIENE-SAMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           TNRIENE SAMVFCCCSWLVADE LAWKIISILEKH+L ST
Sbjct: 639 TNRIENESSAMVFCCCSWLVADEILAWKIISILEKHDLGST 679

[Close]

BLAST of Lsi10G002420.1 vs. NCBI nr
Match: gi|46360445|gb|AAS80153.1| (ACT11D09.9 [Cucumis melo])

HSP 1 Score: 498.8 bits (1283), Expect = 6.1e-138
Identity = 249/281 (88.61%), Postives = 263/281 (93.59%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKHVILVHGEKPKMA LKERIHSELGIPCHDPANNETVSISSTLS+KAEASS
Sbjct: 428 MDLVKFLSPKHVILVHGEKPKMAVLKERIHSELGIPCHDPANNETVSISSTLSIKAEASS 487

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
           MFIQSCSTPNFKFLKRNL DKID +LKDLS KA  TS+M IR+CSNPHFKHLN+NLDAKF
Sbjct: 488 MFIQSCSTPNFKFLKRNLIDKIDPDLKDLSYKAVRTSNMLIRECSNPHFKHLNRNLDAKF 547

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
           DSSLS GPELQVSDDRVNEG++VMENGKKTK +HQDELLLLLGEQEHEVRFA+CRPIYFG
Sbjct: 548 DSSLSGGPELQVSDDRVNEGILVMENGKKTKALHQDELLLLLGEQEHEVRFAHCRPIYFG 607

Query: 181 NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRT 240
           +LD  HV+D LSRKS+WLSQL  KLS+ELSDRNVQNLGEYLQVES TLSICSKENCPYRT
Sbjct: 608 SLDEIHVMDSLSRKSLWLSQLSFKLSTELSDRNVQNLGEYLQVESITLSICSKENCPYRT 667

Query: 241 TNRIENES-AMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           TNRIENES AMVFCCCSWLVADE LAWKIISILEKH+L ST
Sbjct: 668 TNRIENESTAMVFCCCSWLVADEILAWKIISILEKHDLGST 708

[Close]

BLAST of Lsi10G002420.1 vs. NCBI nr
Match: gi|778673818|ref|XP_011650067.1| (PREDICTED: cleavage and polyadenylation specificity factor subunit 3-II isoform X1 [Cucumis sativus])

HSP 1 Score: 486.9 bits (1252), Expect = 2.4e-134
Identity = 244/281 (86.83%), Postives = 260/281 (92.53%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSPKHVILVHGEKPKMA LKERIHSELGIPCHDPANNETVSISSTLSVKAEASS
Sbjct: 399 MDLVKFLSPKHVILVHGEKPKMAVLKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 458

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
           MFIQSCSTPNFKFLKRNL   ID +LKDLS KAE TS+M IR+CSNPHFKHL +NLDAKF
Sbjct: 459 MFIQSCSTPNFKFLKRNL---IDPDLKDLSYKAERTSNMLIRECSNPHFKHLKRNLDAKF 518

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
           DSSLSCGP LQVSDDRVNEG++VMENGKKTK +HQDELLLLLG+QEHEVRFA+CRPIYFG
Sbjct: 519 DSSLSCGPALQVSDDRVNEGILVMENGKKTKALHQDELLLLLGQQEHEVRFAHCRPIYFG 578

Query: 181 NLDVTHVVDCLSRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICSKENCPYRT 240
           +LD  HV+D LSRKS+WLSQL  KLS+ELSDRNVQNLGEYLQVES TLSICSKENCPYRT
Sbjct: 579 SLDEIHVMDSLSRKSLWLSQLSFKLSTELSDRNVQNLGEYLQVESITLSICSKENCPYRT 638

Query: 241 TNRIENES-AMVFCCCSWLVADETLAWKIISILEKHNLSST 281
            +RI+NES AMVFCCCSWLVADE LAWKIISILEKH+L ST
Sbjct: 639 IDRIKNESTAMVFCCCSWLVADEILAWKIISILEKHDLGST 676

[Close]

BLAST of Lsi10G002420.1 vs. NCBI nr
Match: gi|567911691|ref|XP_006448159.1| (hypothetical protein CICLE_v10014563mg [Citrus clementina])

HSP 1 Score: 242.7 bits (618), Expect = 7.9e-61
Identity = 140/288 (48.61%), Postives = 177/288 (61.46%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSP+HVILVHGEKPKMA LKERI SELGI C+DPANNE++ I ST  VKA AS 
Sbjct: 394 MDLVKFLSPQHVILVHGEKPKMATLKERIQSELGIKCYDPANNESMCIPSTHYVKAGASD 453

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
            FI+SC  PNF+FLK    +K  S  K   C  EGT  ++I+                  
Sbjct: 454 AFIRSCMNPNFQFLKSGSEEKSVSGSK---C-TEGTLPLWIK------------------ 513

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
                        D+RV EG++V+E  +K KVVHQDELLL+LGE+ HEV+FA C P+   
Sbjct: 514 -------------DERVAEGILVLEKSEKAKVVHQDELLLMLGEKRHEVQFAYCCPVNVD 573

Query: 181 NLD------VTHVVDCL--SRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICS 240
            L+      +T     L    KS  +  L +KLS +LS+ N+Q+ GE+LQVESF LS+C 
Sbjct: 574 ELEKFTTTSLTPTARMLRDPNKSSLIRLLVAKLSRKLSEGNIQDFGEHLQVESFHLSVCL 633

Query: 241 KENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           K+ CPYR TN +E++    F CC+W  AD+ LA KIIS +E  +L  T
Sbjct: 634 KDTCPYRITNGLEDKPRTAFVCCTWSAADDKLARKIISAMENRDLVET 646

[Close]

BLAST of Lsi10G002420.1 vs. NCBI nr
Match: gi|641841852|gb|KDO60762.1| (hypothetical protein CISIN_1g043917mg, partial [Citrus sinensis])

HSP 1 Score: 241.1 bits (614), Expect = 2.3e-60
Identity = 139/288 (48.26%), Postives = 177/288 (61.46%), Query Frame = 1


Query: 1   MDLVKFLSPKHVILVHGEKPKMAALKERIHSELGIPCHDPANNETVSISSTLSVKAEASS 60
           MDLVKFLSP+HVILVHGEKPKMA LKERI SELGI C+DPANNE++ I ST  VKA AS 
Sbjct: 171 MDLVKFLSPQHVILVHGEKPKMATLKERIQSELGIKCYDPANNESMCIPSTHYVKAGASD 230

Query: 61  MFIQSCSTPNFKFLKRNLNDKIDSNLKDLSCKAEGTSSMFIRKCSNPHFKHLNKNLDAKF 120
            FI+SC  PNF++LK    +K  S  K   C  EGT  ++I+                  
Sbjct: 231 AFIRSCMNPNFQYLKSGSEEKSVSGSK---C-TEGTLPLWIK------------------ 290

Query: 121 DSSLSCGPELQVSDDRVNEGVMVMENGKKTKVVHQDELLLLLGEQEHEVRFANCRPIYFG 180
                        D+RV EG++V+E  +K KVVHQDELLL+LGE+ HEV+FA C P+   
Sbjct: 291 -------------DERVAEGILVLEKSEKAKVVHQDELLLMLGEKRHEVQFAYCCPVNVD 350

Query: 181 NLD------VTHVVDCL--SRKSVWLSQLFSKLSSELSDRNVQNLGEYLQVESFTLSICS 240
            L+      +T     L    KS  +  L +KLS +LS+ N+Q+ GE+LQVESF LS+C 
Sbjct: 351 ELEKFTTTSLTPTARMLRDPNKSSLIRLLVAKLSRKLSEGNIQDFGEHLQVESFHLSVCL 410

Query: 241 KENCPYRTTNRIENESAMVFCCCSWLVADETLAWKIISILEKHNLSST 281
           K+ CPYR TN +E++    F CC+W  AD+ LA KIIS +E  +L  T
Sbjct: 411 KDTCPYRITNGLEDKPRTAFFCCTWSAADDKLARKIISAMENRDLVET 423

The following BLAST results are available for this feature:

BLAST of Lsi10G002420.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CPS3B_ARATH	2.5e-22	34.46	Cleavage and polyadenylation specificity factor subunit 3-II OS=Arabidopsis thal...	[more]
INT11_DICDI	8.6e-07	30.08	Integrator complex subunit 11 homolog OS=Dictyostelium discoideum GN=ints11 PE=3...	[more]
INT11_DANRE	5.5e-06	42.37	Integrator complex subunit 11 OS=Danio rerio GN=cpsf3l PE=2 SV=1	[more]
INT11_CHICK	9.5e-06	40.68	Integrator complex subunit 11 OS=Gallus gallus GN=CPSF3L PE=2 SV=1	[more]
INT11_PONAB	9.5e-06	46.30	Integrator complex subunit 11 OS=Pongo abelii GN=CPSF3L PE=2 SV=2	[more]

BLAST of Lsi10G002420.1 vs. TrEMBL


Match Name	E-value	Identity	Description
Q6E435_CUCME	4.3e-138	88.61	ACT11D09.9 OS=Cucumis melo GN=ACT11D09.9 PE=4 SV=1	[more]
A0A0A0LQW3_CUCSA	1.7e-134	86.83	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G435430 PE=4 SV=1	[more]
V4W5P9_9ROSI	5.5e-61	48.61	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014563mg PE=4 SV=1	[more]
A0A067FBD9_CITSI	1.6e-60	48.26	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g043917mg PE=4 S...	[more]
A0A0D2W0M5_GOSRA	1.3e-54	46.29	Uncharacterized protein (Fragment) OS=Gossypium raimondii GN=B456_012G1826002 PE...	[more]

BLAST of Lsi10G002420.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G01730.1	1.4e-23	34.46	cleavage and polyadenylation specificity factor 73 kDa subunit-II	[more]

BLAST of Lsi10G002420.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118488\|ref\|XP_008459147.1\|	2.5e-139	88.97	PREDICTED: cleavage and polyadenylation specificity factor subunit 3-II isoform ...	[more]
gi\|46360445\|gb\|AAS80153.1\|	6.1e-138	88.61	ACT11D09.9 [Cucumis melo]	[more]
gi\|778673818\|ref\|XP_011650067.1\|	2.4e-134	86.83	PREDICTED: cleavage and polyadenylation specificity factor subunit 3-II isoform ...	[more]
gi\|567911691\|ref\|XP_006448159.1\|	7.9e-61	48.61	hypothetical protein CICLE_v10014563mg [Citrus clementina]	[more]
gi\|641841852\|gb\|KDO60762.1\|	2.3e-60	48.26	hypothetical protein CISIN_1g043917mg, partial [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR011108	RMMBL
IPR001279	Metallo-B-lactamas

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi10G002420	Lsi10G002420	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi10G002420.1.CDS.1	Lsi10G002420.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi10G002420.1	Lsi10G002420.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001279	Metallo-beta-lactamase	unknown	SSF56281	Metallo-hydrolase/oxidoreductase	coord: 2..49 score: 6.35
IPR011108	Zn-dependent metallo-hydrolase, RNA specificity domain	PFAM	PF07521	RMMBL	coord: 1..38 score: 9.
None	No IPR available	PANTHER	PTHR11203	CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR	coord: 1..60 score: 3.2
None	No IPR available	PANTHER	PTHR11203:SF37	INTEGRATOR COMPLEX SUBUNIT 11	coord: 1..60 score: 3.2