Lsi09G017080.1 (mRNA) Bottle gourd (USVL1VR-Ls)

NameLsi09G017080.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionATP binding / protein kinase/ protein tyrosine kinase
Locationchr09 : 25639847 .. 25643326 (+)
Sequence length1989
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCCATGTTTCCTCTCTCTTCCTCTCTCATGGCACTACAACAACATTCTCTGTCATTTCTGCTACAAATTTCTCTCCCCATTCTCCCTCTTCCATCTCTTTAACAATGTCCTCCTCATTTCTCTTTCTCTTCCTCCTCCTCCGCCCTGTCTGCAGCGCCGCCGTCTGCGGCAACCTTCAAATCCCCTTCCCTTTCTCCCTCAACACCACCACTAAAACTCACTCAATCCCATTAATCCCCAACCCATTTCTTCTCTACTGCCTAAACTCCACTTCCCTCTTCCTCAACCTCACCGCCCAATCTTACCGCATCCTCCAATTCCTCTCCGACGCCGTTTTAGTCGACTTCCCCGGCCCATCTCCCTGCCGTCAGTACAACGATTTCAACGCATTTTCCTCTCTTTCTCATAACCCATTTTTCGCCATCTCCGACGACAATCTCCTCGCTCTTTACGACTGTAACGATTCTTCTCTCTGTAAACCCACTTGCCAGAACCTTGTCTTACCCGGATGTGAGTCAACGCCGCCCCACTACTCGCCGGGCTGCTGCTTCCCGCTGACAGACCGTACTCTGTGGCGAAACAGGGGAGATTTTACTCTGTTCTCGAAGCTGGGATGCAGGGGATTCTCGAGCTGGGTGATGGAGAAAGGATGGAGAAGTGGGAAACGAGGAGTGAAATTGGAATGGGGACTTCCGAGGAATTTGACGATTTGTGATAAGAATGGGTTTGTTGTTAATGCTACTGCTGTTACTGATGGGGTTCGTTGTTCTTGTTCTGATGGCTTTGTTGGTGATGGTTTTGCCAATGGATTTGGCTGCTTCAAATGTAAGTAATTAATTTAAGGACAGCTTGATAATTATTTCCACCTCGTTTAGTAATCAGTTTGTTTTTTTGTCTTTGATTTTTAAAAGTTAAGCATGATTCTTCTGGACGTTGTTGAAATGATTTGCATCTTTATCTTTTTTAAATATAAAGAGTTGAACTCTTTAGCTAAATTTCAAAAATAAAAATAAAGTTTTAAAAGCTATTTTTTTTTTTAATTTTTAAAAATTGGCTTAATTTTTTAAGACATTAGTAAAACGTAAGTAATAAAATAAAAAAATTTAGAAGTGGAAGAACTGACTTAATTTTTAATGACAAATGCACATCAGTTTTTATTTTTTGTTTTTAAAATGTAAGTCTATTTTCTTTCGATTTTTTATAATGATTTGCATATTTAGTATAAGAATTGAATTTTTAGGCAAATTTCAAAAACAAAAAAACAAGTTTTTTAAAAACTACTTTTCTTTTTTAATTTTCAAAATTTAACTTGGTTTTGTGATGCTTTTTTTTAATTTCTTTTGATTTGTGTTTTAACTATATGTTTCTCTTGATCTTTTCTTGATGTAGCTTGTGTTAAGAACGGGCATCAGGAGTATGGTAGTAGTTGCAATACCAAACTACATCGTGAAAAGCAGCTTGTTATCTTTACAGGTATTTCATTACTTTGTTATATATTTTTACCAATGTTTTAAAAAGTCAAGTCAAGTTTTGAAAACTAAGAAAAGTAGTTCTTAAAAACTTGTTTTTGTTTTTAGAATTTAGCTAAGAATTCAATTCTTTTACTTAAAAAAGAGGTAAAACATTATAAGAAAATGAGAGGAATAAACTCAATTTTCTAAAACAAAAAACAAATAACCAGACCTAGTTTGGTAACTATTTAGTTTTTAGTTTTTTAGTTTTTGAAAATTAAGCCTATTTCCTCCCAATTTCTTACCATGGTTTTCATCTATCTTGTAAAAGAGTTAATTCTAAGCCAAATTCCAAAAACAAAAAGAAAATTGTAAAACTATTTTTTTTAGTTAAAAAAAACATGAATTAGTTATTGAAAACTTTGGTAGAAAGTAAATAATAAAACAAGAAATTTAAAGGTGGAAATTGGTGTTTACAGACATAATTTTCAAAAACTTAAAACCAAATGATTGTCAAATGAGGCCTAAATAGACACCAAAGGGGCCAAAAAAAAATAGTTTTAAAAATTTGTTTCGATTTTTGGAATTAGAAAGAGTGCCTAATTGGTAATGATGAAAAGCATGGTAAAGAACTTATAAGAAAACCAGCACAAATAACCATTTTCAAAAATCAAAAACTAAAAACAAAATTATTATAAAAGCTTCTAGAACTGAGTGAGTATTGTATTTAGCTGTGTGAACATTTTCCATTACAGAAAAATTTATATTGATGCAATTTGAACCCATCTATTAATTATTACTAATGAAAAATTGTTATTATATGGTAAATGTGCAGGAGTTCTTGCTCCTCTTTTCATTATAGCCTCTATCATTGGACTTTTTTGCATACTAAGACGACCAATCAAACAATCCACACTAAACTCTTCCCACACCCAATTTCACACCAATGCTTTGCTCCAAAAGGCTTGCAGGACTCATTTATTCACTTACCATGAGCTACAACAAGCCACCAGAGGATTTCAGGACAATGCCAAGCTCGTCGACAGCCGGAATGGAGCAATCTTCGCCGGCGTACTCGGGGACGGATCAAGAGTAGCCGTTCACCGGCTGCAATGCGAGAACGAAGGCGACGTTATGAACGTCCTATCCCAAATCGAGGTTTTGTATGTACTTGCACACAAACATATCGCCCACATACTTGGCTGCTGCATCGATCCGGGATGCGCTCCGTTGGTCGTTTACGAGCATCCTGACAACGATACATTGGAGAAACATTTGCATCACCAGAAAGAAACCGACCGAACCGAACCGACGCTCGATTGGTATAGGAGATTGAGAATAGCTGCTGACACAGCTAGTGTTCTTGCATTCCTACAATGTGAAGTGTCTCCCCCAATTTTCCACAACCATCTCGAATCGTGTCATATCTTTCTCGATACGAATTTCTCGAGTAAAGTTTCAGGATTCGGGTTACTAAGCTCTCCGACCGAAGACAAATCTCACCGTCTCGAAGCCCCGTCTTTCCACAACAATGATGTTTATGACTTTGGAGTTGTGTTACTTGAAATGGTAACAGGGTTAAAGAACTCAGATCTCCCAATGGTAGCATTGCAAAAGATTAGAATTGGGAAGCTAGAAGAGGTTGTGGATCCACTTCTATACTATCATGAGAAACCTCCTCAGAGCAAAGAGCAAATAGAGATAGTAGCAGACCTTGCCACAAGGTGCTTGCTTTTCGGCCGAGACGGGAAGTTGCGAATGAGCGACGTTTCGAAGGAGTTGACGCATGTAATGAAAGAAAACGTCGTCGTCGATGGAGATAGCACGAGAGGGCCTGCAATAGAGGAAACTTTCTCCAATTCAAGCCTTCTTCAAATGATATCAATGTCTCCTGATTCAATCCTTGCTCCATGAATAGTTTCAATTCAGGCAGAAGTTTAATCCAAAAAACATGTTCATTTATGAGAACTGTGCATTGGTTGTTTAGAATTTAATATATAAATATATATATGAACTGATAAAGCA

mRNA sequence

ATTTTCCATGTTTCCTCTCTCTTCCTCTCTCATGGCACTACAACAACATTCTCTGTCATTTCTGCTACAAATTTCTCTCCCCATTCTCCCTCTTCCATCTCTTTAACAATGTCCTCCTCATTTCTCTTTCTCTTCCTCCTCCTCCGCCCTGTCTGCAGCGCCGCCGTCTGCGGCAACCTTCAAATCCCCTTCCCTTTCTCCCTCAACACCACCACTAAAACTCACTCAATCCCATTAATCCCCAACCCATTTCTTCTCTACTGCCTAAACTCCACTTCCCTCTTCCTCAACCTCACCGCCCAATCTTACCGCATCCTCCAATTCCTCTCCGACGCCGTTTTAGTCGACTTCCCCGGCCCATCTCCCTGCCGTCAGTACAACGATTTCAACGCATTTTCCTCTCTTTCTCATAACCCATTTTTCGCCATCTCCGACGACAATCTCCTCGCTCTTTACGACTGTAACGATTCTTCTCTCTGTAAACCCACTTGCCAGAACCTTGTCTTACCCGGATGTGAGTCAACGCCGCCCCACTACTCGCCGGGCTGCTGCTTCCCGCTGACAGACCGTACTCTGTGGCGAAACAGGGGAGATTTTACTCTGTTCTCGAAGCTGGGATGCAGGGGATTCTCGAGCTGGGTGATGGAGAAAGGATGGAGAAGTGGGAAACGAGGAGTGAAATTGGAATGGGGACTTCCGAGGAATTTGACGATTTGTGATAAGAATGGGTTTGTTGTTAATGCTACTGCTGTTACTGATGGGGTTCGTTGTTCTTGTTCTGATGGCTTTGTTGGTGATGGAGTTCTTGCTCCTCTTTTCATTATAGCCTCTATCATTGGACTTTTTTGCATACTAAGACGACCAATCAAACAATCCACACTAAACTCTTCCCACACCCAATTTCACACCAATGCTTTGCTCCAAAAGGCTTGCAGGACTCATTTATTCACTTACCATGAGCTACAACAAGCCACCAGAGGATTTCAGGACAATGCCAAGCTCGTCGACAGCCGGAATGGAGCAATCTTCGCCGGCGTACTCGGGGACGGATCAAGAGTAGCCGTTCACCGGCTGCAATGCGAGAACGAAGGCGACGTTATGAACGTCCTATCCCAAATCGAGGTTTTGTATGTACTTGCACACAAACATATCGCCCACATACTTGGCTGCTGCATCGATCCGGGATGCGCTCCGTTGGTCGTTTACGAGCATCCTGACAACGATACATTGGAGAAACATTTGCATCACCAGAAAGAAACCGACCGAACCGAACCGACGCTCGATTGGTATAGGAGATTGAGAATAGCTGCTGACACAGCTAGTGTTCTTGCATTCCTACAATGTGAAGTGTCTCCCCCAATTTTCCACAACCATCTCGAATCGTGTCATATCTTTCTCGATACGAATTTCTCGAGTAAAGTTTCAGGATTCGGGTTACTAAGCTCTCCGACCGAAGACAAATCTCACCGTCTCGAAGCCCCGTCTTTCCACAACAATGATGTTTATGACTTTGGAGTTGTGTTACTTGAAATGGTAACAGGGTTAAAGAACTCAGATCTCCCAATGGTAGCATTGCAAAAGATTAGAATTGGGAAGCTAGAAGAGGTTGTGGATCCACTTCTATACTATCATGAGAAACCTCCTCAGAGCAAAGAGCAAATAGAGATAGTAGCAGACCTTGCCACAAGGTGCTTGCTTTTCGGCCGAGACGGGAAGTTGCGAATGAGCGACGTTTCGAAGGAGTTGACGCATGTAATGAAAGAAAACGTCGTCGTCGATGGAGATAGCACGAGAGGGCCTGCAATAGAGGAAACTTTCTCCAATTCAAGCCTTCTTCAAATGATATCAATGTCTCCTGATTCAATCCTTGCTCCATGAATAGTTTCAATTCAGGCAGAAGTTTAATCCAAAAAACATGTTCATTTATGAGAACTGTGCATTGGTTGTTTAGAATTTAATATATAAATATATATATGAACTGATAAAGCA

Coding sequence (CDS)

ATTTTCCATGTTTCCTCTCTCTTCCTCTCTCATGGCACTACAACAACATTCTCTGTCATTTCTGCTACAAATTTCTCTCCCCATTCTCCCTCTTCCATCTCTTTAACAATGTCCTCCTCATTTCTCTTTCTCTTCCTCCTCCTCCGCCCTGTCTGCAGCGCCGCCGTCTGCGGCAACCTTCAAATCCCCTTCCCTTTCTCCCTCAACACCACCACTAAAACTCACTCAATCCCATTAATCCCCAACCCATTTCTTCTCTACTGCCTAAACTCCACTTCCCTCTTCCTCAACCTCACCGCCCAATCTTACCGCATCCTCCAATTCCTCTCCGACGCCGTTTTAGTCGACTTCCCCGGCCCATCTCCCTGCCGTCAGTACAACGATTTCAACGCATTTTCCTCTCTTTCTCATAACCCATTTTTCGCCATCTCCGACGACAATCTCCTCGCTCTTTACGACTGTAACGATTCTTCTCTCTGTAAACCCACTTGCCAGAACCTTGTCTTACCCGGATGTGAGTCAACGCCGCCCCACTACTCGCCGGGCTGCTGCTTCCCGCTGACAGACCGTACTCTGTGGCGAAACAGGGGAGATTTTACTCTGTTCTCGAAGCTGGGATGCAGGGGATTCTCGAGCTGGGTGATGGAGAAAGGATGGAGAAGTGGGAAACGAGGAGTGAAATTGGAATGGGGACTTCCGAGGAATTTGACGATTTGTGATAAGAATGGGTTTGTTGTTAATGCTACTGCTGTTACTGATGGGGTTCGTTGTTCTTGTTCTGATGGCTTTGTTGGTGATGGAGTTCTTGCTCCTCTTTTCATTATAGCCTCTATCATTGGACTTTTTTGCATACTAAGACGACCAATCAAACAATCCACACTAAACTCTTCCCACACCCAATTTCACACCAATGCTTTGCTCCAAAAGGCTTGCAGGACTCATTTATTCACTTACCATGAGCTACAACAAGCCACCAGAGGATTTCAGGACAATGCCAAGCTCGTCGACAGCCGGAATGGAGCAATCTTCGCCGGCGTACTCGGGGACGGATCAAGAGTAGCCGTTCACCGGCTGCAATGCGAGAACGAAGGCGACGTTATGAACGTCCTATCCCAAATCGAGGTTTTGTATGTACTTGCACACAAACATATCGCCCACATACTTGGCTGCTGCATCGATCCGGGATGCGCTCCGTTGGTCGTTTACGAGCATCCTGACAACGATACATTGGAGAAACATTTGCATCACCAGAAAGAAACCGACCGAACCGAACCGACGCTCGATTGGTATAGGAGATTGAGAATAGCTGCTGACACAGCTAGTGTTCTTGCATTCCTACAATGTGAAGTGTCTCCCCCAATTTTCCACAACCATCTCGAATCGTGTCATATCTTTCTCGATACGAATTTCTCGAGTAAAGTTTCAGGATTCGGGTTACTAAGCTCTCCGACCGAAGACAAATCTCACCGTCTCGAAGCCCCGTCTTTCCACAACAATGATGTTTATGACTTTGGAGTTGTGTTACTTGAAATGGTAACAGGGTTAAAGAACTCAGATCTCCCAATGGTAGCATTGCAAAAGATTAGAATTGGGAAGCTAGAAGAGGTTGTGGATCCACTTCTATACTATCATGAGAAACCTCCTCAGAGCAAAGAGCAAATAGAGATAGTAGCAGACCTTGCCACAAGGTGCTTGCTTTTCGGCCGAGACGGGAAGTTGCGAATGAGCGACGTTTCGAAGGAGTTGACGCATGTAATGAAAGAAAACGTCGTCGTCGATGGAGATAGCACGAGAGGGCCTGCAATAGAGGAAACTTTCTCCAATTCAAGCCTTCTTCAAATGATATCAATGTCTCCTGATTCAATCCTTGCTCCATGA

Protein sequence

IFHVSSLFLSHGTTTTFSVISATNFSPHSPSSISLTMSSSFLFLFLLLRPVCSAAVCGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVDFPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTPPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNLTICDKNGFVVNATAVTDGVRCSCSDGFVGDGVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSNSSLLQMISMSPDSILAP
BLAST of Lsi09G017080.1 vs. Swiss-Prot
Match: Y2685_ARATH (Probably inactive receptor-like protein kinase At2g46850 OS=Arabidopsis thaliana GN=At2g46850 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.2e-140
Identity = 288/656 (43.90%), Postives = 390/656 (59.45%), Query Frame = 1

Query: 35  LTMSSSFLFLFLLL---------------RPVCSAAVCGNLQIPFPFSLNTTTKTHSIPL 94
           L  SSS LFL LLL               + + S   CGN  + FPF L++++   +   
Sbjct: 6   LPSSSSALFLLLLLLLTLQTLTSISLSQPQALRSPEKCGNFSVSFPFQLSSSSSAAA--- 65

Query: 95  IPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVDFPGPSPCRQYNDFNAFSSLSHNP 154
               F L C NS++LFL++  QSYRI++F +D +LVDFP    CRQ+ND  +F   S N 
Sbjct: 66  ----FRLSCENSSTLFLHINHQSYRIIEFFTDGLLVDFPSSPSCRQFNDLRSFP-FSANQ 125

Query: 155 FFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTPPHYSPG----CCFPLTDRTLWRN 214
           FF+IS +N++ LYDC DSSLCK  C+   L GC+      + G    CC+PL+D + WR 
Sbjct: 126 FFSISFENVIGLYDCEDSSLCKFGCETNDLFGCDGREEDETSGGDIGCCYPLSDHSAWRV 185

Query: 215 RGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNL--TICDKNGFVVNATAVTD 274
             DF++FS+ GCRGFSSW++ +G   GKRGVKLEW +PRN    ICD+    VNATA+  
Sbjct: 186 GDDFSVFSRYGCRGFSSWLVPRGTNRGKRGVKLEWAIPRNSPEAICDREARTVNATAIEG 245

Query: 275 GVRCSCSDGFVGD--------------------------------------GVLAPLFII 334
            VRC C DGFVGD                                      GVLAPLFI+
Sbjct: 246 SVRCVCRDGFVGDGFLHGTGCLKSCFKDGKELYGDKCKIKKHNGKKLTVLAGVLAPLFIL 305

Query: 335 ASIIGLFCILRRPI-----KQSTLNSSHTQFHTNALLQKACRTHLFTYHELQQATRGFQD 394
            S++ LFC+L+RP+     +Q  ++++ T  ++ +  +   +T LFTY EL++AT+GFQD
Sbjct: 306 GSLLALFCLLKRPVTSHKDQQFDISTTTTTTNSVSFRKGYNKTRLFTYRELEEATKGFQD 365

Query: 395 NAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHIAHILGC 454
           + KL   + G I++G L +G+RV VH++ CEN+ + M + SQI+ L  + H+++A I+G 
Sbjct: 366 SQKLTQGKTGTIYSGNLTNGTRVIVHKVLCENQIEFMEISSQIDHLSAVLHRNLARIIGF 425

Query: 455 CIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLAFLQCEV 514
           C+D G  PLVVYE+P N +L          DR    LDW +R+ I A+ A +LA LQ E 
Sbjct: 426 CMDIGYNPLVVYEYPVNGSL---------GDRLRLGLDWCKRVNIVAEVAGLLALLQYEN 485

Query: 515 SPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPSFHNNDVYDFGVVLLE 574
            PPI H ++ S +IFLD +F +KV+GFGL      D S            +YDF V+LLE
Sbjct: 486 YPPILHTNISSGNIFLDEDFQAKVTGFGLQRKQRIDTS------------MYDFAVLLLE 545

Query: 575 MVTGLKNSDLPMV-ALQKIRIGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGR 626
           +VTGLK  +  +  ALQKIR GKLEE+VDP +Y+HE+P   +EQI +VAD+ATRC+LFG 
Sbjct: 546 IVTGLKQREETVTQALQKIRSGKLEEIVDPSMYFHEQPVAFREQIGLVADIATRCVLFGG 605

BLAST of Lsi09G017080.1 vs. Swiss-Prot
Match: WAKLO_ARATH (Wall-associated receptor kinase-like 14 OS=Arabidopsis thaliana GN=WAKL14 PE=2 SV=2)

HSP 1 Score: 170.2 bits (430), Expect = 7.0e-41
Identity = 115/347 (33.14%), Postives = 181/347 (52.16%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTH---LFTYHELQQ 326
           G +   F++A++   F   RR   +ST   SH       LL +A        F Y E+++
Sbjct: 289 GTVGGAFLLAALAFFFFCKRR---RSTPLRSHLS--AKRLLSEAAGNSSVAFFPYKEIEK 348

Query: 327 ATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKH 386
           AT GF +  KL     G ++ G L +   VA+ RL+  +   +  V+++I++L  ++H +
Sbjct: 349 ATDGFSEKQKLGIGAYGTVYRGKLQNDEWVAIKRLRHRDSESLDQVMNEIKLLSSVSHPN 408

Query: 387 IAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVL 446
           +  +LGCCI+ G  P++VYE+  N TL +HL   + +      L W  RL +A  TA  +
Sbjct: 409 LVRLLGCCIEQG-DPVLVYEYMPNGTLSEHLQRDRGSG-----LPWTLRLTVATQTAKAI 468

Query: 447 AFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPS-------- 506
           A+L   ++PPI+H  ++S +I LD +F+SKV+ FGL      + SH   AP         
Sbjct: 469 AYLHSSMNPPIYHRDIKSTNILLDYDFNSKVADFGLSRLGMTESSHISTAPQGTPGYLDP 528

Query: 507 -----FH---NNDVYDFGVVLLEMVTGLKNSD---------LPMVALQKIRIGKLEEVVD 566
                FH    +DVY FGVVL E++TGLK  D         L  +A+ KI  G ++E++D
Sbjct: 529 QYHQCFHLSDKSDVYSFGVVLAEIITGLKVVDFTRPHTEINLAALAVDKIGSGCIDEIID 588

Query: 567 PLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           P+L   +    +   I  VA+LA RCL F  D +  M++V+ EL  +
Sbjct: 589 PILDL-DLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQI 623

BLAST of Lsi09G017080.1 vs. Swiss-Prot
Match: WAKLR_ARATH (Wall-associated receptor kinase-like 21 OS=Arabidopsis thaliana GN=WAKL21 PE=2 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 1.4e-38
Identity = 112/349 (32.09%), Postives = 183/349 (52.44%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTHL--FTYHELQQA 326
           G+L  + +I  +I  +       KQS  +S         L + A  + +  +TY E+++A
Sbjct: 255 GILVGVLVIVVLIATYFFRN---KQSA-SSERASIANRLLCELAGNSSVPFYTYKEIEKA 314

Query: 327 TRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHI 386
           T  F D   L     G ++AG   + S VA+ RL+ ++   +  V+++I++L  ++H ++
Sbjct: 315 TDSFSDKNMLGTGAYGTVYAGEFPNSSCVAIKRLKHKDTTSIDQVVNEIKLLSSVSHPNL 374

Query: 387 AHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLA 446
             +LGCC   G  P +VYE   N TL +HL H+    R +P L W  RL IA  TA+ +A
Sbjct: 375 VRLLGCCFADG-EPFLVYEFMPNGTLYQHLQHE----RGQPPLSWQLRLAIACQTANAIA 434

Query: 447 FLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGL--LSSPTE-DKSHRLEA-------- 506
            L   V+PPI+H  ++S +I LD  F+SK+S FGL  L   T+ + SH   A        
Sbjct: 435 HLHSSVNPPIYHRDIKSSNILLDHEFNSKISDFGLSRLGMSTDFEASHISTAPQGTPGYL 494

Query: 507 -PSFH-------NNDVYDFGVVLLEMVTGLKNSD---------LPMVALQKIRIGKLEEV 566
            P +H        +DVY FGVVL+E+++G K  D         L  +A+ +I  G++ ++
Sbjct: 495 DPQYHQDFQLSDKSDVYSFGVVLVEIISGFKVIDFTRPYSEVNLASLAVDRIGRGRVVDI 554

Query: 567 VDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           +DP L   E  P+    I  +A+LA RCL F R+ +  M +++++L  +
Sbjct: 555 IDPCL-NKEINPKMFASIHNLAELAFRCLSFHRNMRPTMVEITEDLHRI 593

BLAST of Lsi09G017080.1 vs. Swiss-Prot
Match: WAKLM_ARATH (Wall-associated receptor kinase-like 17 OS=Arabidopsis thaliana GN=WAKL17 PE=3 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 1.3e-34
Identity = 88/296 (29.73%), Postives = 150/296 (50.68%), Query Frame = 1

Query: 312 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 371
           +  +FT  EL++AT  F +N  L     G ++ G+L DG  VAV + +  +E  +   ++
Sbjct: 428 KARIFTSKELEKATENFSENRVLGHGGQGTVYKGMLVDGRTVAVKKSKVIDEDKLQEFIN 487

Query: 372 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 431
           ++ +L  + H+H+  +LGCC++    P++VYE   N  L KH+H ++  D    T+ W  
Sbjct: 488 EVVILSQINHRHVVKLLGCCLETE-VPILVYEFIINGNLFKHIHEEEADDY---TMIWGM 547

Query: 432 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRL 491
           RLRIA D A  L++L    S PI+H  ++S +I LD  + +KV+ FG   S T D++H  
Sbjct: 548 RLRIAVDIAGALSYLHSAASSPIYHRDIKSTNILLDEKYRAKVADFGTSRSVTIDQTHWT 607

Query: 492 EA---------PSFH-------NNDVYDFGVVLLEMVTG------LKNSDLPMVALQKIR 551
                      P ++        +DVY FGV+L E++TG      ++N+   +   +  R
Sbjct: 608 TVISGTVGYVDPEYYRSSQYTEKSDVYSFGVILAELITGDKPVIMVQNTQEIIALAEHFR 667

Query: 552 IGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           +   E  +  ++    +     EQ+  VA+LA +CL      +  M +V  EL  +
Sbjct: 668 VAMKERRLSDIMDARIRDDSKPEQVMAVANLAMKCLSSRGRNRPNMREVFTELERI 719

BLAST of Lsi09G017080.1 vs. Swiss-Prot
Match: WAKLA_ARATH (Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana GN=WAKL1 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-34
Identity = 112/353 (31.73%), Postives = 178/353 (50.42%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNA--LLQKACRTH--------LF 326
           G+L  L  +   +GLF + +   K+  +N S   F  N   LL++   T         +F
Sbjct: 358 GILIGLSGLVFFVGLFWLFKLIKKRRNINRSKKFFKRNGGLLLKQQLTTKDGNVEMSKIF 417

Query: 327 TYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVL 386
           +  EL++AT  F  +  L     G ++ G+L DGS VAV R +  +E  +   +++I +L
Sbjct: 418 SSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDKMEEFINEIVLL 477

Query: 387 YVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIA 446
             + H++I  +LGCC++    P++VYE+  N  L K LH + +    + T+ W  RLRIA
Sbjct: 478 SQINHRNIVKLLGCCLETE-VPILVYEYIPNGDLFKRLHDESD----DYTMTWEVRLRIA 537

Query: 447 ADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSH--RLEAP 506
            + A  L ++    S PIFH  +++ +I LD  + +KVS FG   S T D++H   L A 
Sbjct: 538 IEIAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQTHLTTLVAG 597

Query: 507 SF--------------HNNDVYDFGVVLLEMVTG------LKNSDLPMVALQKIRIGKLE 566
           +F              H +DVY FGVVL+E++TG      +++ +   +A   +   K  
Sbjct: 598 TFGYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKEN 657

Query: 567 EVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLR--MSDVSKELTHV 586
            V+D ++    K     EQ+  VA LA +CL   R GK R  M +VS EL  +
Sbjct: 658 RVID-IIDIRIKDESKLEQVMAVAKLARKCL--NRKGKNRPNMKEVSNELERI 702

BLAST of Lsi09G017080.1 vs. TrEMBL
Match: A5BPY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_012720 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 4.1e-181
Identity = 328/614 (53.42%), Postives = 423/614 (68.89%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG++ I FPF LN+++ + + P     F L C+NST+LFLN+ + SYR+LQF SD +LVD
Sbjct: 22  CGSIHIAFPFHLNSSSNSPAWPS-SYAFRLSCVNSTTLFLNIASNSYRVLQFFSDGILVD 81

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCEST- 176
           FPG + CRQYND N+F   S N  F IS DN + LYDC DSSLC+  C+  V+P C+S  
Sbjct: 82  FPGATSCRQYNDLNSFR-FSGNDHFGISIDNFIGLYDCEDSSLCRADCEINVMPACDSNG 141

Query: 177 ----PPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWG 236
                   SP CC+ L+D  +W+    F++FS+ GCRGFS W+++ G   GKRGVKLEW 
Sbjct: 142 NGNDSSRTSPACCYALSDGGVWQTGNGFSVFSQFGCRGFSCWLLQPGTNQGKRGVKLEWA 201

Query: 237 LPRNLT--ICDKNGFVVNATAVTDGVRCSCSDGFVGDG---------------------- 296
           +P+N +  +C  N F+VNAT+V  G+RC C DGFVGDG                      
Sbjct: 202 VPKNSSQGVCAINAFMVNATSVQQGIRCMCRDGFVGDGFAHGLGCSKSCIKDGREANGKD 261

Query: 297 ----------------VLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKAC 356
                           VLAP+FIIAS+IGLFC+ +R +K  T +     + +    +KAC
Sbjct: 262 CNTERRNEKKVVILAGVLAPVFIIASLIGLFCLFKRHVKSGTFDPDQAHYQSTISFRKAC 321

Query: 357 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 416
           RT LFT+HEL++ATRGF+D  KLVDS NG +++GVLGDGS VAVH++QC NE D++ VLS
Sbjct: 322 RTQLFTFHELEEATRGFEDGQKLVDSSNGTLYSGVLGDGSHVAVHKVQCGNERDLIQVLS 381

Query: 417 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 476
           ++EVL  + H+++A +LGCCID G   LVVYE+P N TLE+HLH  +  +     LDWY+
Sbjct: 382 RVEVLSAVLHRNMARLLGCCIDSGYTALVVYEYPANGTLEEHLHQSRGKNL---CLDWYK 441

Query: 477 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKS--- 536
           RL+IAA+TAS+L+FLQ E+SPPIFH+ L+S  IFLD +FS K++GFGLLSS   D +   
Sbjct: 442 RLKIAAETASILSFLQHEISPPIFHHDLKSGCIFLDHDFSIKIAGFGLLSSALGDGTQSY 501

Query: 537 HRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPP 596
           H      FH NDVY+ GVVLLE++ G +  D P VALQKI  GKLEE+VDP+LYYHE+PP
Sbjct: 502 HNSGGSCFHRNDVYNLGVVLLEIIAGSRVLDPPTVALQKIGSGKLEEIVDPVLYYHEQPP 561

Query: 597 QSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSN 623
             +EQIE+VADLATRCLLFG DGKL M DV++EL H+MKE+  +DG S RGPA+EETFSN
Sbjct: 562 FRREQIEVVADLATRCLLFGGDGKLGMMDVTRELVHMMKES--MDGCSKRGPALEETFSN 621

BLAST of Lsi09G017080.1 vs. TrEMBL
Match: M5X404_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021129mg PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 6.9e-181
Identity = 332/612 (54.25%), Postives = 417/612 (68.14%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG LQ+PFPF LN +  +     + + F L CLNST+LFLN+ ++SYR+L+F SD +LVD
Sbjct: 45  CGQLQLPFPFHLNKSCSS-----VSDAFHLSCLNSTALFLNIGSESYRVLEFFSDGLLVD 104

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTP 176
           FPG S CRQYND N+F  L  N  F +S +N++ LYDC DSSLCK  C+   LPGC+   
Sbjct: 105 FPGSSSCRQYNDLNSFDFLG-NDHFGVSVENVIGLYDCEDSSLCKTECETNDLPGCDGN- 164

Query: 177 PHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNL 236
            + SP CC+PL+D ++W     F++FSK GCRGFSSWV+++G   GKRGVKLEW +PRN 
Sbjct: 165 VNGSPACCYPLSDHSVWHLGEKFSVFSKFGCRGFSSWVVQRGSNLGKRGVKLEWAVPRNS 224

Query: 237 T--ICDKNGFVVNATAVTDGVRCSCSDGFVGD---------------------------- 296
           +  +C  N ++ NAT V  GVRC C DGF+GD                            
Sbjct: 225 SKGVCASNAYITNATVVEAGVRCICEDGFLGDGFATGEGCIKSCIKDRKEAYGNDCLKKK 284

Query: 297 ----------GVLAPLFIIASIIGLFCILRRPIKQSTLN-SSHTQFHTNALLQKACRTHL 356
                     GVLAP+FIIAS+I L C+L+ P K  T + +    F +    +KA RT L
Sbjct: 285 RGGKKLVILAGVLAPVFIIASLIALLCLLKYPAKPGTFDPAQKPDFQSTISFRKASRTRL 344

Query: 357 FTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEV 416
           FTYHEL++AT+GF++  KL+   NG IFAGVLGDGS +AVH+++CENE D++ VLSQIEV
Sbjct: 345 FTYHELEEATKGFEEGQKLLSGNNGTIFAGVLGDGSHIAVHKVECENEKDLIQVLSQIEV 404

Query: 417 LYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRI 476
           L  + H++IA +LGCCID    PL+VYE+P N TLE+HLH   +       LDWY+RL I
Sbjct: 405 LSAVLHRNIARLLGCCIDLAYTPLLVYEYPANSTLEEHLH---QRGGQNVALDWYKRLSI 464

Query: 477 AADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTED--KSHRLEA 536
           AA+TASVLAFLQ E+SPPIFH  L+  +IF+D NFSSKV+GFGLL +   D  +SH  E 
Sbjct: 465 AAETASVLAFLQYEISPPIFHCDLKPAYIFIDENFSSKVAGFGLLITSLGDGSQSHNHED 524

Query: 537 PSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSKEQ 596
             FH NDVY FGV+LLE++ G    DLP VALQKIR GKLEE+VDP LYYHE+P   +EQ
Sbjct: 525 SRFHKNDVYAFGVMLLEIIAGSNCLDLPTVALQKIRSGKLEEIVDPHLYYHEQPSYRREQ 584

Query: 597 IEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSNSSLLQ 626
           IEIVADLA RCLLFG DGKL M DV++EL H+ +++   DG S RGPA+EETFSNSSLLQ
Sbjct: 585 IEIVADLAMRCLLFGGDGKLGMYDVARELVHIRRDS--SDGGSKRGPALEETFSNSSLLQ 644

BLAST of Lsi09G017080.1 vs. TrEMBL
Match: A0A0A0KDN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G095930 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 8.5e-179
Identity = 321/360 (89.17%), Postives = 336/360 (93.33%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTHLFTYHELQQATR 326
           GVLAPLFIIAS++GLFCIL+RPIKQ+TLNSSHT    NALLQKACRTHLFTYHELQQATR
Sbjct: 281 GVLAPLFIIASLVGLFCILKRPIKQTTLNSSHT----NALLQKACRTHLFTYHELQQATR 340

Query: 327 GFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHIAH 386
           GF+DNA+LVDSRNGAIFAGVLGDGSRV VHRLQCEN+ DVM+VLSQIEVLYVLAHKH+AH
Sbjct: 341 GFEDNARLVDSRNGAIFAGVLGDGSRVVVHRLQCENKDDVMSVLSQIEVLYVLAHKHVAH 400

Query: 387 ILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLAFL 446
           ILGCCIDP    LVVYEHPDNDTLEKHLHH K    T+ TLDWYRRL+IA +TASVLAFL
Sbjct: 401 ILGCCIDPDNPLLVVYEHPDNDTLEKHLHHHK---GTKQTLDWYRRLKIATETASVLAFL 460

Query: 447 QCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPSFHNNDVYDFGV 506
           QCEVSPPIFHNHLESCHIFLDTNFSSK+ GFGL S+PTEDKSH LEA SFHNNDVYDFGV
Sbjct: 461 QCEVSPPIFHNHLESCHIFLDTNFSSKILGFGLQSTPTEDKSHPLEASSFHNNDVYDFGV 520

Query: 507 VLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLL 566
           VLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPP SKEQIEIVADLATRCLL
Sbjct: 521 VLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPHSKEQIEIVADLATRCLL 580

Query: 567 FGRDGKLRMSDVSKELTHVMKENVV-VDGDSTRGPAIEETFSNSSLLQMISMSPDSILAP 626
           FGRDGKLRMSDVSKELTHVMKENVV VDG STRGP+IEETFSNSSLLQMISMSPDSILAP
Sbjct: 581 FGRDGKLRMSDVSKELTHVMKENVVFVDGGSTRGPSIEETFSNSSLLQMISMSPDSILAP 633

BLAST of Lsi09G017080.1 vs. TrEMBL
Match: F6I2Z6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0048g02390 PE=3 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 7.2e-178
Identity = 322/609 (52.87%), Postives = 418/609 (68.64%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG++ I FPF LN+++ + + P     F L C+NST+LFLN+ + SYR+LQF SD +LVD
Sbjct: 38  CGSIHIAFPFHLNSSSNSPAWPS-SYAFRLSCVNSTTLFLNIASNSYRVLQFFSDGILVD 97

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCEST- 176
           FPG + CRQYND N+F   S N  F IS DN + LYDC DSSLC+  C+  V+P C+S  
Sbjct: 98  FPGATSCRQYNDLNSFR-FSGNDHFGISIDNFIGLYDCEDSSLCRADCEINVMPACDSNG 157

Query: 177 ----PPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWG 236
                   SP CC+ L+D  +W+    F++FS+ GCRGFS W+++ G   GKRGVKLEW 
Sbjct: 158 NGNDSSRTSPACCYALSDGGVWQTGNGFSVFSQFGCRGFSCWLLQPGTNQGKRGVKLEWA 217

Query: 237 LPRNLT--ICDKNGFVVNATAVTDGVRCSCSDGFVGDG---------------------- 296
           +P+N +  +C  N F+VNAT+V  G+RC C DGFVGDG                      
Sbjct: 218 VPKNSSQGVCAINAFMVNATSVQQGIRCMCRDGFVGDGFAHGLGCSKSCIKDGREANGKD 277

Query: 297 ----------------VLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKAC 356
                           VLAP+FIIAS+IGLFC+ +R +K  T +     + +    +KAC
Sbjct: 278 CNTERRNEKKVVILAGVLAPVFIIASLIGLFCLFKRHVKSGTFDPDQAHYQSTISFRKAC 337

Query: 357 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 416
           RT LFT+HEL++ATRGF+D  KLVDS NG +++GVLGDGS VAVH++QC NE D++ VLS
Sbjct: 338 RTQLFTFHELEEATRGFEDGQKLVDSSNGTLYSGVLGDGSHVAVHKVQCGNERDLIQVLS 397

Query: 417 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 476
           ++EVL  + H+++A +LGCCID G   LVVYE+P N TLE+HLH  +  +     LDWY+
Sbjct: 398 RVEVLSAVLHRNMARLLGCCIDSGYTALVVYEYPANGTLEEHLHQSRGKNL---CLDWYK 457

Query: 477 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKS--- 536
           RL+IAA+TAS+L+FLQ E+SPPIFH+ L+S  IFLD +FS K++GFGLLSS   D +   
Sbjct: 458 RLKIAAETASILSFLQHEISPPIFHHDLKSGCIFLDHDFSIKIAGFGLLSSALGDGTQSY 517

Query: 537 HRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPP 596
           H      FH NDVY+ GVVLLE++ G +  D P VALQKI  GKLEE+VDP+LYYHE+PP
Sbjct: 518 HNSGGSCFHRNDVYNLGVVLLEIIAGSRVLDPPTVALQKIGSGKLEEIVDPVLYYHEQPP 577

Query: 597 QSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSN 618
             +EQIE+VADLATRCLLFG DGKL M DV++EL H+MKE+  +DG S RGPA+EETFSN
Sbjct: 578 FRREQIEVVADLATRCLLFGGDGKLGMMDVTRELVHMMKES--MDGCSKRGPALEETFSN 637

BLAST of Lsi09G017080.1 vs. TrEMBL
Match: A0A061DTY7_THECC (Probably inactive receptor-like protein kinase OS=Theobroma cacao GN=TCM_005018 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 2.1e-177
Identity = 333/614 (54.23%), Postives = 420/614 (68.40%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CGNL IPFPF LNT+  +     + N F L CLNST+L+L++  +SY IL+F  D +LVD
Sbjct: 40  CGNLHIPFPFHLNTSCAS-----VSNAFHLSCLNSTTLYLHIGIESYGILEFFPDGILVD 99

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTP 176
           FPG S CRQYND NAF   + N +F IS DN++ LYDC DSSLCK  C+   LPGC+   
Sbjct: 100 FPGSSTCRQYNDLNAFG-FAGNDYFGISGDNVIGLYDCEDSSLCKADCETTDLPGCDGNS 159

Query: 177 PHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNL 236
              S  CC+ L+D T+W     F+ FSK GCRGFSSWV+ +G  +G+RGVKLEW +PRN 
Sbjct: 160 GG-SLACCYSLSDHTIWHYGDGFSSFSKFGCRGFSSWVVPRGTNTGERGVKLEWAIPRNT 219

Query: 237 T--ICDKNGFVVNATAVTDGVRCSCSDGFVGD---------------------------- 296
           +  +C  N  VVNAT V  GVRCSC DGFVGD                            
Sbjct: 220 SGGVCASNADVVNATTVEAGVRCSCQDGFVGDGFANGAGCLKSCIKEGQEVYGEECDSRR 279

Query: 297 ----------GVLAPLFIIASIIGLFCILRRPIKQST-LNSSHTQFHTNALLQKACRTHL 356
                     GVLAP FI+AS+    CIL+RP+K    L+ +H  FH+    +KAC T L
Sbjct: 280 HSQRKLVIVAGVLAPAFILASLFLFLCILKRPVKPGAFLDQAH--FHSTISFRKACGTRL 339

Query: 357 FTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEV 416
           F+Y EL++ATR F+D  KLVD  NG I AGVLGDGS +AV ++QC+NE D+++VLS IE+
Sbjct: 340 FSYRELEEATRVFEDGQKLVDGTNGTIHAGVLGDGSHIAVQKVQCDNERDLIHVLSIIEL 399

Query: 417 LYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRI 476
           L  + H+++A +LGCCID G + +VVYE+P N TLE+HL H +     +  LDWY+RL I
Sbjct: 400 LSAVLHRNLARLLGCCIDSGYSLMVVYEYPANGTLEEHLQHSR---GQKFGLDWYKRLSI 459

Query: 477 AADTASVLAFLQCEVSPPIFHNHLESC-HIFLDTNFSSKVSGFGLLSSPTEDKS---HRL 536
           AA+TASVLA+LQ E+SPPIFH+ L+S  +IFLD +FS KV+GF LLSS   D S   +  
Sbjct: 460 AAETASVLAYLQHEISPPIFHHGLKSSGYIFLDADFSVKVAGFALLSSSLGDGSDLCNNY 519

Query: 537 EAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSK 596
           E P  H NDVYDFG++LLE+++G K SD+P VALQKI+ GK+EE+VDP LYYHE+P   +
Sbjct: 520 ENPHIHKNDVYDFGLLLLEIISGSKYSDMPSVALQKIKSGKIEEIVDPSLYYHEQPIFRR 579

Query: 597 EQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSNSSL 626
           EQIEIVAD+ATRCLLFG DGK+ M DV++EL H+ KE+  +DG S RGPA+EETFSNSSL
Sbjct: 580 EQIEIVADIATRCLLFGGDGKIGMFDVARELVHIAKES--IDGGSKRGPALEETFSNSSL 639

BLAST of Lsi09G017080.1 vs. TAIR10
Match: AT2G46850.1 (AT2G46850.1 Protein kinase superfamily protein)

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 288/656 (43.90%), Postives = 390/656 (59.45%), Query Frame = 1

Query: 35  LTMSSSFLFLFLLL---------------RPVCSAAVCGNLQIPFPFSLNTTTKTHSIPL 94
           L  SSS LFL LLL               + + S   CGN  + FPF L++++   +   
Sbjct: 6   LPSSSSALFLLLLLLLTLQTLTSISLSQPQALRSPEKCGNFSVSFPFQLSSSSSAAA--- 65

Query: 95  IPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVDFPGPSPCRQYNDFNAFSSLSHNP 154
               F L C NS++LFL++  QSYRI++F +D +LVDFP    CRQ+ND  +F   S N 
Sbjct: 66  ----FRLSCENSSTLFLHINHQSYRIIEFFTDGLLVDFPSSPSCRQFNDLRSFP-FSANQ 125

Query: 155 FFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTPPHYSPG----CCFPLTDRTLWRN 214
           FF+IS +N++ LYDC DSSLCK  C+   L GC+      + G    CC+PL+D + WR 
Sbjct: 126 FFSISFENVIGLYDCEDSSLCKFGCETNDLFGCDGREEDETSGGDIGCCYPLSDHSAWRV 185

Query: 215 RGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNL--TICDKNGFVVNATAVTD 274
             DF++FS+ GCRGFSSW++ +G   GKRGVKLEW +PRN    ICD+    VNATA+  
Sbjct: 186 GDDFSVFSRYGCRGFSSWLVPRGTNRGKRGVKLEWAIPRNSPEAICDREARTVNATAIEG 245

Query: 275 GVRCSCSDGFVGD--------------------------------------GVLAPLFII 334
            VRC C DGFVGD                                      GVLAPLFI+
Sbjct: 246 SVRCVCRDGFVGDGFLHGTGCLKSCFKDGKELYGDKCKIKKHNGKKLTVLAGVLAPLFIL 305

Query: 335 ASIIGLFCILRRPI-----KQSTLNSSHTQFHTNALLQKACRTHLFTYHELQQATRGFQD 394
            S++ LFC+L+RP+     +Q  ++++ T  ++ +  +   +T LFTY EL++AT+GFQD
Sbjct: 306 GSLLALFCLLKRPVTSHKDQQFDISTTTTTTNSVSFRKGYNKTRLFTYRELEEATKGFQD 365

Query: 395 NAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHIAHILGC 454
           + KL   + G I++G L +G+RV VH++ CEN+ + M + SQI+ L  + H+++A I+G 
Sbjct: 366 SQKLTQGKTGTIYSGNLTNGTRVIVHKVLCENQIEFMEISSQIDHLSAVLHRNLARIIGF 425

Query: 455 CIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLAFLQCEV 514
           C+D G  PLVVYE+P N +L          DR    LDW +R+ I A+ A +LA LQ E 
Sbjct: 426 CMDIGYNPLVVYEYPVNGSL---------GDRLRLGLDWCKRVNIVAEVAGLLALLQYEN 485

Query: 515 SPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPSFHNNDVYDFGVVLLE 574
            PPI H ++ S +IFLD +F +KV+GFGL      D S            +YDF V+LLE
Sbjct: 486 YPPILHTNISSGNIFLDEDFQAKVTGFGLQRKQRIDTS------------MYDFAVLLLE 545

Query: 575 MVTGLKNSDLPMV-ALQKIRIGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGR 626
           +VTGLK  +  +  ALQKIR GKLEE+VDP +Y+HE+P   +EQI +VAD+ATRC+LFG 
Sbjct: 546 IVTGLKQREETVTQALQKIRSGKLEEIVDPSMYFHEQPVAFREQIGLVADIATRCVLFGG 605

BLAST of Lsi09G017080.1 vs. TAIR10
Match: AT2G23450.2 (AT2G23450.2 Protein kinase superfamily protein)

HSP 1 Score: 170.2 bits (430), Expect = 3.9e-42
Identity = 115/347 (33.14%), Postives = 181/347 (52.16%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTH---LFTYHELQQ 326
           G +   F++A++   F   RR   +ST   SH       LL +A        F Y E+++
Sbjct: 289 GTVGGAFLLAALAFFFFCKRR---RSTPLRSHLS--AKRLLSEAAGNSSVAFFPYKEIEK 348

Query: 327 ATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKH 386
           AT GF +  KL     G ++ G L +   VA+ RL+  +   +  V+++I++L  ++H +
Sbjct: 349 ATDGFSEKQKLGIGAYGTVYRGKLQNDEWVAIKRLRHRDSESLDQVMNEIKLLSSVSHPN 408

Query: 387 IAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVL 446
           +  +LGCCI+ G  P++VYE+  N TL +HL   + +      L W  RL +A  TA  +
Sbjct: 409 LVRLLGCCIEQG-DPVLVYEYMPNGTLSEHLQRDRGSG-----LPWTLRLTVATQTAKAI 468

Query: 447 AFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPS-------- 506
           A+L   ++PPI+H  ++S +I LD +F+SKV+ FGL      + SH   AP         
Sbjct: 469 AYLHSSMNPPIYHRDIKSTNILLDYDFNSKVADFGLSRLGMTESSHISTAPQGTPGYLDP 528

Query: 507 -----FH---NNDVYDFGVVLLEMVTGLKNSD---------LPMVALQKIRIGKLEEVVD 566
                FH    +DVY FGVVL E++TGLK  D         L  +A+ KI  G ++E++D
Sbjct: 529 QYHQCFHLSDKSDVYSFGVVLAEIITGLKVVDFTRPHTEINLAALAVDKIGSGCIDEIID 588

Query: 567 PLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           P+L   +    +   I  VA+LA RCL F  D +  M++V+ EL  +
Sbjct: 589 PILDL-DLDAWTLSSIHTVAELAFRCLAFHSDMRPTMTEVADELEQI 623

BLAST of Lsi09G017080.1 vs. TAIR10
Match: AT5G66790.1 (AT5G66790.1 Protein kinase superfamily protein)

HSP 1 Score: 162.5 bits (410), Expect = 8.2e-40
Identity = 112/349 (32.09%), Postives = 183/349 (52.44%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTHL--FTYHELQQA 326
           G+L  + +I  +I  +       KQS  +S         L + A  + +  +TY E+++A
Sbjct: 255 GILVGVLVIVVLIATYFFRN---KQSA-SSERASIANRLLCELAGNSSVPFYTYKEIEKA 314

Query: 327 TRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVLYVLAHKHI 386
           T  F D   L     G ++AG   + S VA+ RL+ ++   +  V+++I++L  ++H ++
Sbjct: 315 TDSFSDKNMLGTGAYGTVYAGEFPNSSCVAIKRLKHKDTTSIDQVVNEIKLLSSVSHPNL 374

Query: 387 AHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIAADTASVLA 446
             +LGCC   G  P +VYE   N TL +HL H+    R +P L W  RL IA  TA+ +A
Sbjct: 375 VRLLGCCFADG-EPFLVYEFMPNGTLYQHLQHE----RGQPPLSWQLRLAIACQTANAIA 434

Query: 447 FLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGL--LSSPTE-DKSHRLEA-------- 506
            L   V+PPI+H  ++S +I LD  F+SK+S FGL  L   T+ + SH   A        
Sbjct: 435 HLHSSVNPPIYHRDIKSSNILLDHEFNSKISDFGLSRLGMSTDFEASHISTAPQGTPGYL 494

Query: 507 -PSFH-------NNDVYDFGVVLLEMVTGLKNSD---------LPMVALQKIRIGKLEEV 566
            P +H        +DVY FGVVL+E+++G K  D         L  +A+ +I  G++ ++
Sbjct: 495 DPQYHQDFQLSDKSDVYSFGVVLVEIISGFKVIDFTRPYSEVNLASLAVDRIGRGRVVDI 554

Query: 567 VDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           +DP L   E  P+    I  +A+LA RCL F R+ +  M +++++L  +
Sbjct: 555 IDPCL-NKEINPKMFASIHNLAELAFRCLSFHRNMRPTMVEITEDLHRI 593

BLAST of Lsi09G017080.1 vs. TAIR10
Match: AT4G31100.1 (AT4G31100.1 wall-associated kinase, putative)

HSP 1 Score: 149.4 bits (376), Expect = 7.2e-36
Identity = 88/296 (29.73%), Postives = 150/296 (50.68%), Query Frame = 1

Query: 312 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 371
           +  +FT  EL++AT  F +N  L     G ++ G+L DG  VAV + +  +E  +   ++
Sbjct: 428 KARIFTSKELEKATENFSENRVLGHGGQGTVYKGMLVDGRTVAVKKSKVIDEDKLQEFIN 487

Query: 372 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 431
           ++ +L  + H+H+  +LGCC++    P++VYE   N  L KH+H ++  D    T+ W  
Sbjct: 488 EVVILSQINHRHVVKLLGCCLETE-VPILVYEFIINGNLFKHIHEEEADDY---TMIWGM 547

Query: 432 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRL 491
           RLRIA D A  L++L    S PI+H  ++S +I LD  + +KV+ FG   S T D++H  
Sbjct: 548 RLRIAVDIAGALSYLHSAASSPIYHRDIKSTNILLDEKYRAKVADFGTSRSVTIDQTHWT 607

Query: 492 EA---------PSFH-------NNDVYDFGVVLLEMVTG------LKNSDLPMVALQKIR 551
                      P ++        +DVY FGV+L E++TG      ++N+   +   +  R
Sbjct: 608 TVISGTVGYVDPEYYRSSQYTEKSDVYSFGVILAELITGDKPVIMVQNTQEIIALAEHFR 667

Query: 552 IGKLEEVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHV 586
           +   E  +  ++    +     EQ+  VA+LA +CL      +  M +V  EL  +
Sbjct: 668 VAMKERRLSDIMDARIRDDSKPEQVMAVANLAMKCLSSRGRNRPNMREVFTELERI 719

BLAST of Lsi09G017080.1 vs. TAIR10
Match: AT1G16120.1 (AT1G16120.1 wall associated kinase-like 1)

HSP 1 Score: 149.1 bits (375), Expect = 9.3e-36
Identity = 112/353 (31.73%), Postives = 178/353 (50.42%), Query Frame = 1

Query: 267 GVLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNA--LLQKACRTH--------LF 326
           G+L  L  +   +GLF + +   K+  +N S   F  N   LL++   T         +F
Sbjct: 358 GILIGLSGLVFFVGLFWLFKLIKKRRNINRSKKFFKRNGGLLLKQQLTTKDGNVEMSKIF 417

Query: 327 TYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEVL 386
           +  EL++AT  F  +  L     G ++ G+L DGS VAV R +  +E  +   +++I +L
Sbjct: 418 SSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDKMEEFINEIVLL 477

Query: 387 YVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRIA 446
             + H++I  +LGCC++    P++VYE+  N  L K LH + +    + T+ W  RLRIA
Sbjct: 478 SQINHRNIVKLLGCCLETE-VPILVYEYIPNGDLFKRLHDESD----DYTMTWEVRLRIA 537

Query: 447 ADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSH--RLEAP 506
            + A  L ++    S PIFH  +++ +I LD  + +KVS FG   S T D++H   L A 
Sbjct: 538 IEIAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQTHLTTLVAG 597

Query: 507 SF--------------HNNDVYDFGVVLLEMVTG------LKNSDLPMVALQKIRIGKLE 566
           +F              H +DVY FGVVL+E++TG      +++ +   +A   +   K  
Sbjct: 598 TFGYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKEN 657

Query: 567 EVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLR--MSDVSKELTHV 586
            V+D ++    K     EQ+  VA LA +CL   R GK R  M +VS EL  +
Sbjct: 658 RVID-IIDIRIKDESKLEQVMAVAKLARKCL--NRKGKNRPNMKEVSNELERI 702

BLAST of Lsi09G017080.1 vs. NCBI nr
Match: gi|659119726|ref|XP_008459811.1| (PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Cucumis melo])

HSP 1 Score: 982.6 bits (2539), Expect = 3.1e-283
Identity = 501/632 (79.27%), Postives = 533/632 (84.34%), Query Frame = 1

Query: 33  ISLTMSSSFLFLFLLLRPVCSAAVCGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNST 92
           ISL + S FLFL L +     ++VCGNL+IPFPFSLNTT      P IP+PFLLYCLNST
Sbjct: 16  ISLPIPSLFLFLLLFVPCSVHSSVCGNLEIPFPFSLNTTIH----PSIPDPFLLYCLNST 75

Query: 93  SLFLNLTAQSYRILQFLSDAVLVDFPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALY 152
           SLFLNLT QSYRILQFLSDAVLVDFPGPSPCR YNDFN+FS +SH+PFFAISDDNL ALY
Sbjct: 76  SLFLNLTLQSYRILQFLSDAVLVDFPGPSPCRHYNDFNSFSPVSHSPFFAISDDNLFALY 135

Query: 153 DCNDSSLCKPTCQNLVLPGCESTPPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSS 212
           DCNDSSLCKPTCQNLVLP C+S   +Y P CC+PL+DR+LWRNRGDF++FSK+GCRGFSS
Sbjct: 136 DCNDSSLCKPTCQNLVLPSCDS---NYPPACCYPLSDRSLWRNRGDFSVFSKMGCRGFSS 195

Query: 213 WVMEKGWRSGKRGVKLEWGLPRNLTICDKNGFVVNATAVTDGVRCSCS------------ 272
           WV EKGWR GKRGVKLEWGLPRNLT CD+NGFVVNAT V+DGVRCSCS            
Sbjct: 196 WVFEKGWRMGKRGVKLEWGLPRNLTSCDENGFVVNATNVSDGVRCSCSDGFVGDGYANGF 255

Query: 273 --------------------------DGFVGDGVLAPLFIIASIIGLFCILRRPIKQSTL 332
                                     +  +  GVLAPLFIIAS++GLFCIL+RPIKQ+TL
Sbjct: 256 GCFKSCVKNGRQEYGSSCNTKQRREKELVIFTGVLAPLFIIASLVGLFCILKRPIKQTTL 315

Query: 333 NSSHTQFHTNALLQKACRTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVA 392
           NSSHT    NALLQKACRT LFTYHELQQATRGF+DNAKLVDSRNGAIFAGVLGDGSRV 
Sbjct: 316 NSSHT----NALLQKACRTRLFTYHELQQATRGFEDNAKLVDSRNGAIFAGVLGDGSRVV 375

Query: 393 VHRLQCENEGDVMNVLSQIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHL 452
           VHRLQCEN+ DVMNVLSQIEVLYVLAHKH+AHILGCCIDP   PLVVYEHPDNDTLEKHL
Sbjct: 376 VHRLQCENKADVMNVLSQIEVLYVLAHKHVAHILGCCIDPDNPPLVVYEHPDNDTLEKHL 435

Query: 453 HHQKETDRTEPTLDWYRRLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKV 512
           HHQK     + TLDWYRRL+IA +TASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKV
Sbjct: 436 HHQK---GIKQTLDWYRRLKIATETASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKV 495

Query: 513 SGFGLLSSPTEDKSHRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLE 572
            GFGLLSSPTEDKSH LEA SFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLE
Sbjct: 496 LGFGLLSSPTEDKSHPLEASSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLE 555

Query: 573 EVVDPLLYYHEKPPQSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVV-VD 626
           EVVDPLLYYHEKPPQ KEQIEIVADLATRCLLFGRDGKLRMSDVSKELTH+MKENVV VD
Sbjct: 556 EVVDPLLYYHEKPPQIKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHLMKENVVFVD 615

BLAST of Lsi09G017080.1 vs. NCBI nr
Match: gi|1009142804|ref|XP_015888921.1| (PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Ziziphus jujuba])

HSP 1 Score: 652.1 bits (1681), Expect = 9.6e-184
Identity = 334/610 (54.75%), Postives = 422/610 (69.18%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG  +IPFPF LN+   +     +PN F L C NST+LFL +  +SYR+L+F SD ++VD
Sbjct: 47  CGETKIPFPFHLNSPCGS-----VPNAFNLSCENSTALFLKVGTESYRVLEFFSDGIVVD 106

Query: 117 FPGPSP-CRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCEST 176
           FPGPS  CRQYND N+F   + + +F +S DN++ LYDC DSSLCK  C+ + LPGC+  
Sbjct: 107 FPGPSSSCRQYNDLNSFD-FTESDYFGVSVDNVIGLYDCEDSSLCKAECETIDLPGCDGK 166

Query: 177 PPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRN 236
               SP CC+PL+D ++WR    F++FSK GCRGFSSWV+     SG+RGVKLEW +PRN
Sbjct: 167 DSS-SPACCYPLSDHSVWRIGDKFSVFSKFGCRGFSSWVVLPTANSGRRGVKLEWAIPRN 226

Query: 237 LT--ICDKNGFVVNATAVTDGVRCSCSDGFVGDG-------------------------- 296
            +  +C  N  +  ATAV DGVRCSC DGFVG+G                          
Sbjct: 227 SSKAVCAGNADIATATAVEDGVRCSCPDGFVGNGFANGTGCIKSCIKDGREAYGSDCYSK 286

Query: 297 ------------VLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKACRTHL 356
                       VLA +FIIAS+I LF +L+RP+K  T +     FH++   +KACRT L
Sbjct: 287 RHSDKKLVILAGVLASIFIIASLIALFWLLKRPVKPGTYDPHRDHFHSSISFRKACRTRL 346

Query: 357 FTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEV 416
           FTYHEL+ AT+ F++  KL D  NG IFAGVLGDGS +AVH+++CE E D++ VLSQIE+
Sbjct: 347 FTYHELEVATKEFEEGQKLADGNNGTIFAGVLGDGSHIAVHKIKCETERDLIQVLSQIEI 406

Query: 417 LYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRI 476
           L  + H++IAH+LGCCID    PLVVYE+P N TLE+HLH ++E    +  LDW+RRL I
Sbjct: 407 LSAVLHRNIAHLLGCCIDLAYMPLVVYEYPANGTLEEHLHQRREP---KTGLDWFRRLNI 466

Query: 477 AADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKSHRLEAPS 536
           AA+TASVLAFL  E+ PPIFHN L+S +IFLD +FS+K++GFGLLSS  E   + LE   
Sbjct: 467 AAETASVLAFLHYEMCPPIFHNDLKSGYIFLDEHFSAKIAGFGLLSSKQEYSKNTLEGSR 526

Query: 537 FHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSKEQIE 596
           F  NDV DFGV+LLE++ G  + DLP + LQKIR GKLEE+VDPLLYYHE+P   +EQIE
Sbjct: 527 FQKNDVCDFGVLLLEIIAGSNSLDLPTLVLQKIRNGKLEEIVDPLLYYHEQPSFRREQIE 586

Query: 597 IVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSNSSLLQMI 626
           IVADLATRCLLFG DGK+ M DV++EL H+ K +  +DG S RGPA+EETFSNSSLLQMI
Sbjct: 587 IVADLATRCLLFGGDGKIGMVDVARELGHITKGS--IDGGSKRGPALEETFSNSSLLQMI 644

BLAST of Lsi09G017080.1 vs. NCBI nr
Match: gi|645278368|ref|XP_008244195.1| (PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Prunus mume])

HSP 1 Score: 647.5 bits (1669), Expect = 2.4e-182
Identity = 332/612 (54.25%), Postives = 420/612 (68.63%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG LQ+PFPF LN +  +     + + F L CLNST+LFLN+ ++SYR+L+F SD +LVD
Sbjct: 45  CGQLQLPFPFHLNKSCSS-----VSDAFHLSCLNSTALFLNIGSESYRVLEFFSDGLLVD 104

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCESTP 176
           FPG S CRQYND N+F  L  N  F +S +N++ LYDC DSSLCK  C+   LPGC+   
Sbjct: 105 FPGSSSCRQYNDLNSFDFLG-NDHFGVSVENVIGLYDCEDSSLCKTECETNDLPGCDGN- 164

Query: 177 PHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWGLPRNL 236
            + SP CC+PL+D ++W     F++FSK GCRGFSSWV+++G   GKRGVKLEW +P+N 
Sbjct: 165 VNGSPACCYPLSDHSVWHFGEKFSVFSKFGCRGFSSWVVQRGSNLGKRGVKLEWAVPKNS 224

Query: 237 T--ICDKNGFVVNATAVTDGVRCSCSDGFVGD---------------------------- 296
           +  +C  N ++ NAT V  GVRC C DGF+GD                            
Sbjct: 225 SKGVCASNAYITNATVVEAGVRCICEDGFLGDGFATGEGCIKSCIKDRKEAYGNDCLKKK 284

Query: 297 ----------GVLAPLFIIASIIGLFCILRRPIKQSTLN-SSHTQFHTNALLQKACRTHL 356
                     GVLAP+FI+AS+I L C+L+RP K  T + +    F +    +KACRT L
Sbjct: 285 RGGKKLVILAGVLAPVFIVASLIALLCLLKRPAKPGTFDPAQKPHFQSTISFRKACRTRL 344

Query: 357 FTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLSQIEV 416
           FTYHEL++AT+GF++  KL+   NG IFAGVLGDGS +AVH+++CENE D++ VLSQIEV
Sbjct: 345 FTYHELEEATKGFEEGQKLLSGNNGTIFAGVLGDGSHIAVHKVECENEKDLIQVLSQIEV 404

Query: 417 LYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYRRLRI 476
           L  + H++IA +LGCCID    PL+VYE+P N TL++HLH   +       LDWY+RL I
Sbjct: 405 LSAVLHRNIARLLGCCIDLAYTPLLVYEYPANSTLKEHLH---QRGGQNVALDWYKRLSI 464

Query: 477 AADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTED--KSHRLEA 536
           AA+TASVLAFLQ E+SPPIFH  L+S +IF+D NFSSKV+GFGLL S   D  +SH  E 
Sbjct: 465 AAETASVLAFLQYEISPPIFHCDLKSAYIFIDENFSSKVAGFGLLISSLGDGSQSHNHED 524

Query: 537 PSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPPQSKEQ 596
             FH NDVY FGV+LLE++ G    DLP +ALQKIR GKLEE+VDP LYYHE+P   +EQ
Sbjct: 525 SRFHKNDVYAFGVMLLEIIAGSNCLDLPTLALQKIRSGKLEEIVDPHLYYHEQPSYRREQ 584

Query: 597 IEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSNSSLLQ 626
           IEIVADLA RCLLFG DGKL M DV++EL H+ +++   DG S RGPA+EETFSNSSLLQ
Sbjct: 585 IEIVADLAMRCLLFGGDGKLGMYDVARELVHIRRDS--SDGGSKRGPALEETFSNSSLLQ 644

BLAST of Lsi09G017080.1 vs. NCBI nr
Match: gi|147856745|emb|CAN81350.1| (hypothetical protein VITISV_012720 [Vitis vinifera])

HSP 1 Score: 642.9 bits (1657), Expect = 5.8e-181
Identity = 328/614 (53.42%), Postives = 423/614 (68.89%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG++ I FPF LN+++ + + P     F L C+NST+LFLN+ + SYR+LQF SD +LVD
Sbjct: 22  CGSIHIAFPFHLNSSSNSPAWPS-SYAFRLSCVNSTTLFLNIASNSYRVLQFFSDGILVD 81

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCEST- 176
           FPG + CRQYND N+F   S N  F IS DN + LYDC DSSLC+  C+  V+P C+S  
Sbjct: 82  FPGATSCRQYNDLNSFR-FSGNDHFGISIDNFIGLYDCEDSSLCRADCEINVMPACDSNG 141

Query: 177 ----PPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWG 236
                   SP CC+ L+D  +W+    F++FS+ GCRGFS W+++ G   GKRGVKLEW 
Sbjct: 142 NGNDSSRTSPACCYALSDGGVWQTGNGFSVFSQFGCRGFSCWLLQPGTNQGKRGVKLEWA 201

Query: 237 LPRNLT--ICDKNGFVVNATAVTDGVRCSCSDGFVGDG---------------------- 296
           +P+N +  +C  N F+VNAT+V  G+RC C DGFVGDG                      
Sbjct: 202 VPKNSSQGVCAINAFMVNATSVQQGIRCMCRDGFVGDGFAHGLGCSKSCIKDGREANGKD 261

Query: 297 ----------------VLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKAC 356
                           VLAP+FIIAS+IGLFC+ +R +K  T +     + +    +KAC
Sbjct: 262 CNTERRNEKKVVILAGVLAPVFIIASLIGLFCLFKRHVKSGTFDPDQAHYQSTISFRKAC 321

Query: 357 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 416
           RT LFT+HEL++ATRGF+D  KLVDS NG +++GVLGDGS VAVH++QC NE D++ VLS
Sbjct: 322 RTQLFTFHELEEATRGFEDGQKLVDSSNGTLYSGVLGDGSHVAVHKVQCGNERDLIQVLS 381

Query: 417 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 476
           ++EVL  + H+++A +LGCCID G   LVVYE+P N TLE+HLH  +  +     LDWY+
Sbjct: 382 RVEVLSAVLHRNMARLLGCCIDSGYTALVVYEYPANGTLEEHLHQSRGKNL---CLDWYK 441

Query: 477 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKS--- 536
           RL+IAA+TAS+L+FLQ E+SPPIFH+ L+S  IFLD +FS K++GFGLLSS   D +   
Sbjct: 442 RLKIAAETASILSFLQHEISPPIFHHDLKSGCIFLDHDFSIKIAGFGLLSSALGDGTQSY 501

Query: 537 HRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPP 596
           H      FH NDVY+ GVVLLE++ G +  D P VALQKI  GKLEE+VDP+LYYHE+PP
Sbjct: 502 HNSGGSCFHRNDVYNLGVVLLEIIAGSRVLDPPTVALQKIGSGKLEEIVDPVLYYHEQPP 561

Query: 597 QSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSN 623
             +EQIE+VADLATRCLLFG DGKL M DV++EL H+MKE+  +DG S RGPA+EETFSN
Sbjct: 562 FRREQIEVVADLATRCLLFGGDGKLGMMDVTRELVHMMKES--MDGCSKRGPALEETFSN 621

BLAST of Lsi09G017080.1 vs. NCBI nr
Match: gi|731420824|ref|XP_010661521.1| (PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Vitis vinifera])

HSP 1 Score: 642.9 bits (1657), Expect = 5.8e-181
Identity = 328/614 (53.42%), Postives = 423/614 (68.89%), Query Frame = 1

Query: 57  CGNLQIPFPFSLNTTTKTHSIPLIPNPFLLYCLNSTSLFLNLTAQSYRILQFLSDAVLVD 116
           CG++ I FPF LN+++ + + P     F L C+NST+LFLN+ + SYR+LQF SD +LVD
Sbjct: 38  CGSIHIAFPFHLNSSSNSPAWPS-SYAFRLSCVNSTTLFLNIASNSYRVLQFFSDGILVD 97

Query: 117 FPGPSPCRQYNDFNAFSSLSHNPFFAISDDNLLALYDCNDSSLCKPTCQNLVLPGCEST- 176
           FPG + CRQYND N+F   S N  F IS DN + LYDC DSSLC+  C+  V+P C+S  
Sbjct: 98  FPGATSCRQYNDLNSFR-FSGNDHFGISIDNFIGLYDCEDSSLCRADCEINVMPACDSNG 157

Query: 177 ----PPHYSPGCCFPLTDRTLWRNRGDFTLFSKLGCRGFSSWVMEKGWRSGKRGVKLEWG 236
                   SP CC+ L+D  +W+    F++FS+ GCRGFS W+++ G   GKRGVKLEW 
Sbjct: 158 NGNDSSRTSPACCYALSDGGVWQTGNGFSVFSQFGCRGFSCWLLQPGTNQGKRGVKLEWA 217

Query: 237 LPRNLT--ICDKNGFVVNATAVTDGVRCSCSDGFVGDG---------------------- 296
           +P+N +  +C  N F+VNAT+V  G+RC C DGFVGDG                      
Sbjct: 218 VPKNSSQGVCAINAFMVNATSVQQGIRCMCRDGFVGDGFAHGLGCSKSCIKDGREANGKD 277

Query: 297 ----------------VLAPLFIIASIIGLFCILRRPIKQSTLNSSHTQFHTNALLQKAC 356
                           VLAP+FIIAS+IGLFC+ +R +K  T +     + +    +KAC
Sbjct: 278 CNTERRNEKKVVILAGVLAPVFIIASLIGLFCLFKRHVKSGTFDPDQAHYQSTISFRKAC 337

Query: 357 RTHLFTYHELQQATRGFQDNAKLVDSRNGAIFAGVLGDGSRVAVHRLQCENEGDVMNVLS 416
           RT LFT+HEL++ATRGF+D  KLVDS NG +++GVLGDGS VAVH++QC NE D++ VLS
Sbjct: 338 RTQLFTFHELEEATRGFEDGQKLVDSSNGTLYSGVLGDGSHVAVHKVQCGNERDLIQVLS 397

Query: 417 QIEVLYVLAHKHIAHILGCCIDPGCAPLVVYEHPDNDTLEKHLHHQKETDRTEPTLDWYR 476
           ++EVL  + H+++A +LGCCID G   LVVYE+P N TLE+HLH  +  +     LDWY+
Sbjct: 398 RVEVLSAVLHRNMARLLGCCIDSGYTALVVYEYPANGTLEEHLHQSRGKNL---CLDWYK 457

Query: 477 RLRIAADTASVLAFLQCEVSPPIFHNHLESCHIFLDTNFSSKVSGFGLLSSPTEDKS--- 536
           RL+IAA+TAS+L+FLQ E+SPPIFH+ L+S  IFLD +FS K++GFGLLSS   D +   
Sbjct: 458 RLKIAAETASILSFLQHEISPPIFHHDLKSGCIFLDHDFSIKIAGFGLLSSALGDGTQSY 517

Query: 537 HRLEAPSFHNNDVYDFGVVLLEMVTGLKNSDLPMVALQKIRIGKLEEVVDPLLYYHEKPP 596
           H      FH NDVY+ GVVLLE++ G +  D P VALQKI  GKLEE+VDP+LYYHE+PP
Sbjct: 518 HNSGGSCFHRNDVYNLGVVLLEIIAGSRVLDPPTVALQKIGSGKLEEIVDPVLYYHEQPP 577

Query: 597 QSKEQIEIVADLATRCLLFGRDGKLRMSDVSKELTHVMKENVVVDGDSTRGPAIEETFSN 623
             +EQIE+VADLATRCLLFG DGKL M DV++EL H+MKE+  +DG S RGPA+EETFSN
Sbjct: 578 FRREQIEVVADLATRCLLFGGDGKLGMMDVTRELVHMMKES--MDGCSKRGPALEETFSN 637

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Y2685_ARATH2.2e-14043.90Probably inactive receptor-like protein kinase At2g46850 OS=Arabidopsis thaliana... [more]
WAKLO_ARATH7.0e-4133.14Wall-associated receptor kinase-like 14 OS=Arabidopsis thaliana GN=WAKL14 PE=2 S... [more]
WAKLR_ARATH1.4e-3832.09Wall-associated receptor kinase-like 21 OS=Arabidopsis thaliana GN=WAKL21 PE=2 S... [more]
WAKLM_ARATH1.3e-3429.73Wall-associated receptor kinase-like 17 OS=Arabidopsis thaliana GN=WAKL17 PE=3 S... [more]
WAKLA_ARATH1.7e-3431.73Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana GN=WAKL1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A5BPY9_VITVI4.1e-18153.42Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_012720 PE=4 SV=1[more]
M5X404_PRUPE6.9e-18154.25Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021129mg PE=4 SV=1[more]
A0A0A0KDN5_CUCSA8.5e-17989.17Uncharacterized protein OS=Cucumis sativus GN=Csa_6G095930 PE=4 SV=1[more]
F6I2Z6_VITVI7.2e-17852.87Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0048g02390 PE=3 SV=... [more]
A0A061DTY7_THECC2.1e-17754.23Probably inactive receptor-like protein kinase OS=Theobroma cacao GN=TCM_005018 ... [more]
Match NameE-valueIdentityDescription
AT2G46850.11.3e-14143.90 Protein kinase superfamily protein[more]
AT2G23450.23.9e-4233.14 Protein kinase superfamily protein[more]
AT5G66790.18.2e-4032.09 Protein kinase superfamily protein[more]
AT4G31100.17.2e-3629.73 wall-associated kinase, putative[more]
AT1G16120.19.3e-3631.73 wall associated kinase-like 1[more]
Match NameE-valueIdentityDescription
gi|659119726|ref|XP_008459811.1|3.1e-28379.27PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Cucumis mel... [more]
gi|1009142804|ref|XP_015888921.1|9.6e-18454.75PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Ziziphus ju... [more]
gi|645278368|ref|XP_008244195.1|2.4e-18254.25PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Prunus mume... [more]
gi|147856745|emb|CAN81350.1|5.8e-18153.42hypothetical protein VITISV_012720 [Vitis vinifera][more]
gi|731420824|ref|XP_010661521.1|5.8e-18153.42PREDICTED: probably inactive receptor-like protein kinase At2g46850 [Vitis vinif... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0004713protein tyrosine kinase activity
GO:0005524ATP binding
GO:0004672protein kinase activity
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: INTERPRO
TermDefinition
IPR020635Tyr_kinase_cat_dom
IPR011009Kinase-like_dom_sf
IPR001245Ser-Thr/Tyr_kinase_cat_dom
IPR000719Prot_kinase_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004713 protein tyrosine kinase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi09G017080Lsi09G017080gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G017080.1.CDS.1Lsi09G017080.1.CDS.1CDS
Lsi09G017080.1.CDS.2Lsi09G017080.1.CDS.2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi09G017080.1.three_prime_UTR.1Lsi09G017080.1.three_prime_UTR.1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi09G017080.1Lsi09G017080.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 328..612
score: 14
IPR001245Serine-threonine/tyrosine-protein kinase catalytic domainPFAMPF07714Pkinase_Tyrcoord: 338..515
score: 1.7
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 310..582
score: 4.98
IPR020635Tyrosine-protein kinase, catalytic domainSMARTSM00219tyrkin_6coord: 334..582
score: 0.
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 390..567
score: 1.2
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 312..389
score: 8.9
NoneNo IPR availablePANTHERPTHR27005FAMILY NOT NAMEDcoord: 37..622
score: 2.8E
NoneNo IPR availablePANTHERPTHR27005:SF45SUBFAMILY NOT NAMEDcoord: 37..622
score: 2.8E