Lsi08G015630.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G015630.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Clathrin interactor EPSIN 1-like protein
Location	chr08 : 23698047 .. 23700117 (+)
Sequence length	1338

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACGTGACTGGAGTCCATCAAGTTCTTCTGATCATTAATTTTCTTTTCCTTTTTGTTAAAAAGAAATCTCTCACCTTTTTACAGACTACTGAGGTTGATTTGTTTGCTTCGCAACCTGCTTCAGTTTCTGCAACGGTTGACTTTTTTGCCTCTCCTGAGCCAGTTGTACAAACAGAAACCAACTTTGCTGGTGCAGCAAGAGACAGTAAAGCTTCACAACCTGCTTCAGTTTCTACAACAATCGACTTTTTTGCCTCTCCTGAGCCAGTTGTGCAAACAGAAACTAACTTTGTGAATGCAGCAAGCGACATTAAAGCTTCGCAACCTACTTCAGTTTCTGCACCAGTTGACTTATTTGCCTCTCCTGAGCCAGTTGTACAAACGGAAACCAACTTTGCTAGCGCAGCAAGAGACAGTAAAGCTGTGGATCCATTTGCTGCTCTTCCACTTAACTCTTTTGATGGATCAGATCTTCTCGGTGCACCGTCTTTGCATTCCGAACCAGTGTTGTCAGAACCTTCCCAGAATCCTGTTGGTGGCCCATCTAATAATTTAGATGGAAAATCTATCAACAGTTCAGCAGCTCCAGCCAAGAAGAATACTTTCCAGGTCAAATCTGGGATTTGGGCGGATTCACTTAGTCGAGGGTTGATTGATCTTAATATATCTGCTCGTAAGTCTATCTTAACTGTTACAATCTTTTTTACTGACCTTTTTTATCATTCCCATCTTTTCTCCTCTCTCCTTGAACTAATTGAGACATTTCGTAGACAATATTCCGGAATAGAATGTACCATCTTCAGAATTTTATGTTTATTTTCTTCTAAGTTGGGATCTGGAATAGAAGTGTGCTCATTGGATGGGGCTGAATTAACCTATTATGTTGGGGTAAAAGCAAAGTTAAATTATGAGTTAGTCCTTGTCAAATAAGTTCTTGTACTTTAAAAGGTTCCTAACAAGTGTCTGAACTTTCAAGTTTGTGTCTAATAGGCCCCCTAGACTCTAAAATGTGTGCCAATAAGTCCTTCAACTTTGATTACGTGTCAATGAGTCTCCAACTTTCGTTTTTGTGCCCTATAGGTCCTTGACCTATTCGAATACTTTTCAAAATTCACGAACCTATTTGATCTGTTAATTTTAAAACATGTTGAAAATGTTAGGTATCTCTTAAACACAAAATTGAAAGTTCGGGGATCTATTTGACATTTTTAAAGTGTATGAACCTATTAATCATTACACTCACATGAGAAACTATTACACTCTCTTCATCACTTCGTTAACTCACATGAGAAATTGTTCAGATCTTTTGTTAGTTCTCTTGGTGTATTGTCTTGGCTCCCCCAAGGCACTTTTGGGTTGTTTTAATATAGCTCCCTTAATACAAAAGTTGCTTGCTTGCATTGCAGCCAAGAAGGTTTCCCTGGTAGATGTCGGTGTCGTGGGCGGACTGAGCGATTTATCGGACGAGAGGGAGAAAGGACCAGCACCTATTTATCACATGGGACAAGCAATGGGTGCAGGATCTGGTCTTGGTAGGACAGGATCGCAGACGATTGGAGACGATTTCTTTTCACAACTTTCTGGTCAACAATACCAATTTGGTGGTTTCCAGAAGTAAATTTGTTCTTTCACCCCTGCCTCTATTCTTCCTGTTAATCTGCAAAGAGTGTGTGGGAAAACCTCGAGCCTTTTGGTGTGAATGGCTTTTGCATATACGCCACATATTATTGATTCGAGGAAATGCCTTGAATAATATAAAATGGTTGCTGCGTTTCTTGTGGTTCTATATCTTACTGAGTTGCGATGAATGAAAGAATGATGCTTCTTTTTATTAAACATGTTGCAAAAGTAATACTGGAGCAACTTAACTTTATAAAAATGGAAAGAAAGTGATCACAATACCCTGTCTATAAATTATTCTGATGTTATAAAGGGTTTAAATATATGTAGTTGTGACCTTTGAATGTCTAATTACATGTTCATTAGAATTGAACTATGTACGTTTCTGTTCAAGAGTCAATTAGTTTTTTCTGAATGGGTCCAAATATATATCGTTTTATTGTTGTCT

mRNA sequence

ATGCACGTGACTGGAGTCCATCAAGTTCTTCTGATCATTAATTTTCTTTTCCTTTTTGTTAAAAAGAAATCTCTCACCTTTTTACAGACTACTGAGGTTGATTTGTTTGCTTCGCAACCTGCTTCAGTTTCTGCAACGGTTGACTTTTTTGCCTCTCCTGAGCCAGTTGTACAAACAGAAACCAACTTTGCTGGTGCAGCAAGAGACAGTAAAGCTTCACAACCTGCTTCAGTTTCTACAACAATCGACTTTTTTGCCTCTCCTGAGCCAGTTGTGCAAACAGAAACTAACTTTGTGAATGCAGCAAGCGACATTAAAGCTTCGCAACCTACTTCAGTTTCTGCACCAGTTGACTTATTTGCCTCTCCTGAGCCAGTTGTACAAACGGAAACCAACTTTGCTAGCGCAGCAAGAGACAGTAAAGCTGTGGATCCATTTGCTGCTCTTCCACTTAACTCTTTTGATGGATCAGATCTTCTCGGTGCACCGTCTTTGCATTCCGAACCAGTGTTGTCAGAACCTTCCCAGAATCCTGTTGGTGGCCCATCTAATAATTTAGATGGAAAATCTATCAACAGTTCAGCAGCTCCAGCCAAGAAGAATACTTTCCAGGTCAAATCTGGGATTTGGGCGGATTCACTTAGTCGAGGGTTGATTGATCTTAATATATCTGCTCCCAAGAAGGTTTCCCTGGTAGATGTCGGTGTCGTGGGCGGACTGAGCGATTTATCGGACGAGAGGGAGAAAGGACCAGCACCTATTTATCACATGGGACAAGCAATGGGTGCAGGATCTGGTCTTGGTAGGACAGGATCGCAGACGATTGGAGACGATTTCTTTTCACAACTTTCTGGTCAACAATACCAATTTGGTGGTTTCCAGAAGTAAATTTGTTCTTTCACCCCTGCCTCTATTCTTCCTGTTAATCTGCAAAGAGTGTGTGGGAAAACCTCGAGCCTTTTGGTGTGAATGGCTTTTGCATATACGCCACATATTATTGATTCGAGGAAATGCCTTGAATAATATAAAATGGTTGCTGCGTTTCTTGTGGTTCTATATCTTACTGAGTTGCGATGAATGAAAGAATGATGCTTCTTTTTATTAAACATGTTGCAAAAGTAATACTGGAGCAACTTAACTTTATAAAAATGGAAAGAAAGTGATCACAATACCCTGTCTATAAATTATTCTGATGTTATAAAGGGTTTAAATATATGTAGTTGTGACCTTTGAATGTCTAATTACATGTTCATTAGAATTGAACTATGTACGTTTCTGTTCAAGAGTCAATTAGTTTTTTCTGAATGGGTCCAAATATATATCGTTTTATTGTTGTCT

Coding sequence (CDS)

ATGCACGTGACTGGAGTCCATCAAGTTCTTCTGATCATTAATTTTCTTTTCCTTTTTGTTAAAAAGAAATCTCTCACCTTTTTACAGACTACTGAGGTTGATTTGTTTGCTTCGCAACCTGCTTCAGTTTCTGCAACGGTTGACTTTTTTGCCTCTCCTGAGCCAGTTGTACAAACAGAAACCAACTTTGCTGGTGCAGCAAGAGACAGTAAAGCTTCACAACCTGCTTCAGTTTCTACAACAATCGACTTTTTTGCCTCTCCTGAGCCAGTTGTGCAAACAGAAACTAACTTTGTGAATGCAGCAAGCGACATTAAAGCTTCGCAACCTACTTCAGTTTCTGCACCAGTTGACTTATTTGCCTCTCCTGAGCCAGTTGTACAAACGGAAACCAACTTTGCTAGCGCAGCAAGAGACAGTAAAGCTGTGGATCCATTTGCTGCTCTTCCACTTAACTCTTTTGATGGATCAGATCTTCTCGGTGCACCGTCTTTGCATTCCGAACCAGTGTTGTCAGAACCTTCCCAGAATCCTGTTGGTGGCCCATCTAATAATTTAGATGGAAAATCTATCAACAGTTCAGCAGCTCCAGCCAAGAAGAATACTTTCCAGGTCAAATCTGGGATTTGGGCGGATTCACTTAGTCGAGGGTTGATTGATCTTAATATATCTGCTCCCAAGAAGGTTTCCCTGGTAGATGTCGGTGTCGTGGGCGGACTGAGCGATTTATCGGACGAGAGGGAGAAAGGACCAGCACCTATTTATCACATGGGACAAGCAATGGGTGCAGGATCTGGTCTTGGTAGGACAGGATCGCAGACGATTGGAGACGATTTCTTTTCACAACTTTCTGGTCAACAATACCAATTTGGTGGTTTCCAGAAGTAA

Protein sequence

MHVTGVHQVLLIINFLFLFVKKKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVSTTIDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVDLFASPEPVVQTETNFASAARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAAPAKKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMGQAMGAGSGLGRTGSQTIGDDFFSQLSGQQYQFGGFQK

Homology

[Close]

BLAST of Lsi08G015630.1 vs. Swiss-Prot
Match: EPN1_ARATH (Clathrin interactor EPSIN 1 OS=Arabidopsis thaliana GN=EPSIN1 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 8.4e-37
Identity = 111/257 (43.19%), Postives = 143/257 (55.64%), Query Frame = 1


Query: 63  FAGAARDSKASQPASVSTT----IDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVD 122
           FA AA  S ++Q A   +     +D F++ EP V            + ++ PT     VD
Sbjct: 322 FADAAFVSASAQGAEFGSQTQKQVDLFSASEPSVT-----------VSSAPPT-----VD 381

Query: 123 LFASPEPVVQTETNFA--SAARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQ 182
           LFAS E VV  E   +   +      VDPFAA+P+++FDGSD  GA + HS  V + P  
Sbjct: 382 LFASSESVVSPEAKISIPESMATPNIVDPFAAVPMDNFDGSDPFGAFTSHSASVSTGPQA 441

Query: 183 NPVGGPSNNLDGKSINSSAAPA---KKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVD 242
             V G + N       + + P    KK+ FQVKSGIWADSLSRGLIDLNI+APKK SL D
Sbjct: 442 PSVHGSATNTTSPLSFADSKPQHLQKKDPFQVKSGIWADSLSRGLIDLNITAPKKASLAD 501

Query: 243 VGVVGGLSDLSDEREKGPAPIYHMGQAMGAGSGLGRTG---------------SQTIGDD 296
           VGVVG LS  +++  K  A  Y+ G +MGAGSGLG+TG               +  I DD
Sbjct: 502 VGVVGDLS--NEDGNKASAASYYSGWSMGAGSGLGKTGLYSTQQQQQQQQQQQAPDISDD 560

[Close]

BLAST of Lsi08G015630.1 vs. Swiss-Prot
Match: EPN3_ARATH (Clathrin interactor EPSIN 3 OS=Arabidopsis thaliana GN=EPSIN3 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.8e-08
Identity = 56/186 (30.11%), Postives = 81/186 (43.55%), Query Frame = 1


Query: 102 ASDIKASQPTSVSAPVDLF-------ASPEPVVQTETNFASAARDSKAVDPFAALPLNSF 161
           +S  +   PT+ +AP   F         P  V+       S         P AA   ++ 
Sbjct: 724 SSSSQTPYPTTPNAPSGQFDGGNFMTQQPYGVIPQVHGVPSHIPQRTQSGPVAAHGNSNN 783

Query: 162 DGSDLLGAPSLHS-EPVLSEPSQNPVGGPSNNLDGKSINSSAAPAKKNTFQVKSGIWADS 221
              D+     L S E   S+PS  P+ G               P  +  F+ KS IWAD+
Sbjct: 784 VVGDMFSPAGLSSLETSASQPSLTPLTGAIE----------IVPQNQKKFEPKSTIWADT 843

Query: 222 LSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGP-------APIYHMGQAMGAGSG 273
           LSRGL++ NIS PK   L D+GV     +  ++R + P           +MG+AMG+G+G
Sbjct: 844 LSRGLVNFNISGPKTNPLADIGVDFEAINRKEKRLEKPTITQQQVTSTINMGKAMGSGTG 899

[Close]

BLAST of Lsi08G015630.1 vs. Swiss-Prot
Match: EPN2_ARATH (Clathrin interactor EPSIN 2 OS=Arabidopsis thaliana GN=EPSIN2 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.8e-08
Identity = 42/131 (32.06%), Postives = 66/131 (50.38%), Query Frame = 1


Query: 161 GAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAA--------------PAKKNTFQVK 220
           G     S PV  + + N +G   +     S+ SS++              P  +  F+ K
Sbjct: 655 GPQRTQSGPVTLQGNNNVMGDMFSQATPNSLTSSSSHPDLTPLTGAIEIVPPPQKKFEPK 714

Query: 221 SGIWADSLSRGLIDLNISAPKKVSLVDVGV----VGGLSDLSDEREKGPA-PIYHMGQAM 273
           S +WAD+LSRGL++ NIS  K   L D+GV    +       +++   PA    +MG+AM
Sbjct: 715 SSVWADTLSRGLVNFNISGSKTNPLADIGVDFEAINRREKRLEKQTNTPATSTINMGKAM 774

[Close]

BLAST of Lsi08G015630.1 vs. TrEMBL
Match: A0A0A0LKH0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G352400 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-128
Identity = 245/276 (88.77%), Postives = 261/276 (94.57%), Query Frame = 1


Query: 20  VKKKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVS 79
           ++K+  + L+TTEVDLFASQPASVSATVDFF+SP+PVVQT TNFA AARDSKASQPAS S
Sbjct: 346 MEKEPNSPLKTTEVDLFASQPASVSATVDFFSSPDPVVQTGTNFASAARDSKASQPASNS 405

Query: 80  TTIDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVDLFASPEPVVQTETNFASAARD 139
           +TIDFFAS EPVV+TETNFV+AA DIKASQP+SVSAPVDLFASPEPV++TETNFASAARD
Sbjct: 406 STIDFFASSEPVVETETNFVSAARDIKASQPSSVSAPVDLFASPEPVLKTETNFASAARD 465

Query: 140 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAAPAK 199
           SKAVDPFAALPLNSFDGSDL GAPS HSEPV S+PSQ+PVGGPSNNLDGKSINSS APAK
Sbjct: 466 SKAVDPFAALPLNSFDGSDLFGAPSSHSEPVSSQPSQSPVGGPSNNLDGKSINSSIAPAK 525

Query: 200 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMGQ 259
           KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSD SDEREKGPAP YHMGQ
Sbjct: 526 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDFSDEREKGPAPTYHMGQ 585

Query: 260 AMGAGSGLGRTGSQTIGDDFFSQLSGQQYQFGGFQK 296
           AMGAGSGLGRTGSQ +GDDFFSQLSGQQYQFGGFQK
Sbjct: 586 AMGAGSGLGRTGSQALGDDFFSQLSGQQYQFGGFQK 621

[Close]

BLAST of Lsi08G015630.1 vs. TrEMBL
Match: W9RS28_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_019545 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.3e-56
Identity = 150/280 (53.57%), Postives = 177/280 (63.21%), Query Frame = 1


Query: 30  TTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVSTTIDFFA--- 89
           ++EVDLFA            F S  P V+T  N                 T +D FA   
Sbjct: 323 SSEVDLFADAT---------FVSAAPQVETRANS---------------QTEVDLFADAT 382

Query: 90  --SPEPVVQTETNFVNAAS-DIKASQP---TSVSAPVDLFASPEPVVQTETNFASAAR-D 149
             S  P V+T  +       D+ ASQP   +S S+PVDLFA+P+ V Q E      A  +
Sbjct: 383 FVSAPPQVETRASTQTKNDIDLFASQPPISSSASSPVDLFATPDTVSQPEIKSTHTAPIN 442

Query: 150 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINS-SAAPA 209
           +  VDPFAA+PLN+FD SD  GA + HS    SEPSQN V   S+N+ G +I S S + A
Sbjct: 443 ANIVDPFAAVPLNNFDDSDPFGAFTSHSSSASSEPSQNSVNSGSHNVSGGNIASDSKSSA 502

Query: 210 KKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMG 269
           KK+TFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLS+  DE+EKGPA  Y+MG
Sbjct: 503 KKDTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSEGLDEKEKGPATSYYMG 562

Query: 270 QAMGAGSGLGRTG---SQTIGDDFFSQLSGQQYQFGGFQK 296
           +AMGAGSGLGR+G     T GDDFFS L GQQYQ+GGFQK
Sbjct: 563 RAMGAGSGLGRSGFPSHGTGGDDFFSSLGGQQYQYGGFQK 578

[Close]

BLAST of Lsi08G015630.1 vs. TrEMBL
Match: A0A0D2UGC2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.1e-54
Identity = 143/286 (50.00%), Postives = 178/286 (62.24%), Query Frame = 1


Query: 22  KKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPA---SV 81
           +KS     +T+VDLFA           F ++P  V    +  A    D  ASQPA   + 
Sbjct: 321 EKSAVSTDSTDVDLFAD--------ATFVSAPTKVTHESSPQAQQQVDLFASQPAITPAA 380

Query: 82  STTIDFFASPEPVVQTETNFVNAASDIKASQPT---SVSAPVDLFASPEPVVQTETNFAS 141
           S T+D FA+ +PVVQ     +    D+ ASQP    +VS  VDLF++ +PVVQ       
Sbjct: 381 SPTVDIFAAGDPVVQPH---IKGQVDLFASQPAITPAVSPTVDLFSTTDPVVQPAMAANV 440

Query: 142 AARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVG-GPSNNLDGKSINSS 201
              ++  VDPFAA+PLN+FDGSD+ G+ + HS+  L EP QNP+  G  NN+  KS   S
Sbjct: 441 GPVNANIVDPFAAVPLNNFDGSDIFGSFTTHSDSALEEPKQNPMNDGNLNNMSTKSSQDS 500

Query: 202 AAPAKKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPI 261
            AP KK+ FQVKSGIWADSLSRG+IDLNISAPKKVSL DVG+V GLSD+ DEREKGP   
Sbjct: 501 KAPQKKDAFQVKSGIWADSLSRGIIDLNISAPKKVSLADVGIVCGLSDV-DEREKGPPTS 560

Query: 262 YHMGQAMGAGSGLGRTG----SQTIGDDFFSQLSG-QQYQFGGFQK 296
           ++MG+AMG GSGLG+TG      T  D  FS L   QQYQFG F+K
Sbjct: 561 FYMGRAMGTGSGLGKTGFASTPSTDEDGLFSSLGNHQQYQFGSFKK 594

[Close]

BLAST of Lsi08G015630.1 vs. TrEMBL
Match: A0A0D2TJL0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.1e-54
Identity = 143/286 (50.00%), Postives = 178/286 (62.24%), Query Frame = 1


Query: 22  KKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPA---SV 81
           +KS     +T+VDLFA           F ++P  V    +  A    D  ASQPA   + 
Sbjct: 329 EKSAVSTDSTDVDLFAD--------ATFVSAPTKVTHESSPQAQQQVDLFASQPAITPAA 388

Query: 82  STTIDFFASPEPVVQTETNFVNAASDIKASQPT---SVSAPVDLFASPEPVVQTETNFAS 141
           S T+D FA+ +PVVQ     +    D+ ASQP    +VS  VDLF++ +PVVQ       
Sbjct: 389 SPTVDIFAAGDPVVQPH---IKGQVDLFASQPAITPAVSPTVDLFSTTDPVVQPAMAANV 448

Query: 142 AARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVG-GPSNNLDGKSINSS 201
              ++  VDPFAA+PLN+FDGSD+ G+ + HS+  L EP QNP+  G  NN+  KS   S
Sbjct: 449 GPVNANIVDPFAAVPLNNFDGSDIFGSFTTHSDSALEEPKQNPMNDGNLNNMSTKSSQDS 508

Query: 202 AAPAKKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPI 261
            AP KK+ FQVKSGIWADSLSRG+IDLNISAPKKVSL DVG+V GLSD+ DEREKGP   
Sbjct: 509 KAPQKKDAFQVKSGIWADSLSRGIIDLNISAPKKVSLADVGIVCGLSDV-DEREKGPPTS 568

Query: 262 YHMGQAMGAGSGLGRTG----SQTIGDDFFSQLSG-QQYQFGGFQK 296
           ++MG+AMG GSGLG+TG      T  D  FS L   QQYQFG F+K
Sbjct: 569 FYMGRAMGTGSGLGKTGFASTPSTDEDGLFSSLGNHQQYQFGSFKK 602

[Close]

BLAST of Lsi08G015630.1 vs. TrEMBL
Match: A0A0D2TDP0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.6e-53
Identity = 144/287 (50.17%), Postives = 178/287 (62.02%), Query Frame = 1


Query: 22  KKSLTFLQTTEVDLFASQP-ASVSATVDFFASPEPVVQTETNFAGAARDSKASQPA---S 81
           +KS     +T+VDLFA     S    V   +SP+  V           D  ASQPA   +
Sbjct: 321 EKSAVSTDSTDVDLFADATFVSAPTKVTHESSPQQQV-----------DLFASQPAITPA 380

Query: 82  VSTTIDFFASPEPVVQTETNFVNAASDIKASQPT---SVSAPVDLFASPEPVVQTETNFA 141
            S T+D FA+ +PVVQ     +    D+ ASQP    +VS  VDLF++ +PVVQ      
Sbjct: 381 ASPTVDIFAAGDPVVQPH---IKGQVDLFASQPAITPAVSPTVDLFSTTDPVVQPAMAAN 440

Query: 142 SAARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVG-GPSNNLDGKSINS 201
               ++  VDPFAA+PLN+FDGSD+ G+ + HS+  L EP QNP+  G  NN+  KS   
Sbjct: 441 VGPVNANIVDPFAAVPLNNFDGSDIFGSFTTHSDSALEEPKQNPMNDGNLNNMSTKSSQD 500

Query: 202 SAAPAKKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAP 261
           S AP KK+ FQVKSGIWADSLSRG+IDLNISAPKKVSL DVG+V GLSD+ DEREKGP  
Sbjct: 501 SKAPQKKDAFQVKSGIWADSLSRGIIDLNISAPKKVSLADVGIVCGLSDV-DEREKGPPT 560

Query: 262 IYHMGQAMGAGSGLGRTG----SQTIGDDFFSQLSG-QQYQFGGFQK 296
            ++MG+AMG GSGLG+TG      T  D  FS L   QQYQFG F+K
Sbjct: 561 SFYMGRAMGTGSGLGKTGFASTPSTDEDGLFSSLGNHQQYQFGSFKK 592

[Close]

BLAST of Lsi08G015630.1 vs. TAIR10
Match: AT5G11710.1 (AT5G11710.1 ENTH/VHS family protein)

HSP 1 Score: 155.6 bits (392), Expect = 4.7e-38
Identity = 111/257 (43.19%), Postives = 143/257 (55.64%), Query Frame = 1


Query: 63  FAGAARDSKASQPASVSTT----IDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVD 122
           FA AA  S ++Q A   +     +D F++ EP V            + ++ PT     VD
Sbjct: 322 FADAAFVSASAQGAEFGSQTQKQVDLFSASEPSVT-----------VSSAPPT-----VD 381

Query: 123 LFASPEPVVQTETNFA--SAARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQ 182
           LFAS E VV  E   +   +      VDPFAA+P+++FDGSD  GA + HS  V + P  
Sbjct: 382 LFASSESVVSPEAKISIPESMATPNIVDPFAAVPMDNFDGSDPFGAFTSHSASVSTGPQA 441

Query: 183 NPVGGPSNNLDGKSINSSAAPA---KKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVD 242
             V G + N       + + P    KK+ FQVKSGIWADSLSRGLIDLNI+APKK SL D
Sbjct: 442 PSVHGSATNTTSPLSFADSKPQHLQKKDPFQVKSGIWADSLSRGLIDLNITAPKKASLAD 501

Query: 243 VGVVGGLSDLSDEREKGPAPIYHMGQAMGAGSGLGRTG---------------SQTIGDD 296
           VGVVG LS  +++  K  A  Y+ G +MGAGSGLG+TG               +  I DD
Sbjct: 502 VGVVGDLS--NEDGNKASAASYYSGWSMGAGSGLGKTGLYSTQQQQQQQQQQQAPDISDD 560

[Close]

BLAST of Lsi08G015630.1 vs. TAIR10
Match: AT3G59290.1 (AT3G59290.1 ENTH/VHS family protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 56/186 (30.11%), Postives = 81/186 (43.55%), Query Frame = 1


Query: 102 ASDIKASQPTSVSAPVDLF-------ASPEPVVQTETNFASAARDSKAVDPFAALPLNSF 161
           +S  +   PT+ +AP   F         P  V+       S         P AA   ++ 
Sbjct: 724 SSSSQTPYPTTPNAPSGQFDGGNFMTQQPYGVIPQVHGVPSHIPQRTQSGPVAAHGNSNN 783

Query: 162 DGSDLLGAPSLHS-EPVLSEPSQNPVGGPSNNLDGKSINSSAAPAKKNTFQVKSGIWADS 221
              D+     L S E   S+PS  P+ G               P  +  F+ KS IWAD+
Sbjct: 784 VVGDMFSPAGLSSLETSASQPSLTPLTGAIE----------IVPQNQKKFEPKSTIWADT 843

Query: 222 LSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGP-------APIYHMGQAMGAGSG 273
           LSRGL++ NIS PK   L D+GV     +  ++R + P           +MG+AMG+G+G
Sbjct: 844 LSRGLVNFNISGPKTNPLADIGVDFEAINRKEKRLEKPTITQQQVTSTINMGKAMGSGTG 899

[Close]

BLAST of Lsi08G015630.1 vs. TAIR10
Match: AT2G43160.3 (AT2G43160.3 ENTH/VHS family protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 42/131 (32.06%), Postives = 66/131 (50.38%), Query Frame = 1


Query: 161 GAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAA--------------PAKKNTFQVK 220
           G     S PV  + + N +G   +     S+ SS++              P  +  F+ K
Sbjct: 655 GPQRTQSGPVTLQGNNNVMGDMFSQATPNSLTSSSSHPDLTPLTGAIEIVPPPQKKFEPK 714

Query: 221 SGIWADSLSRGLIDLNISAPKKVSLVDVGV----VGGLSDLSDEREKGPA-PIYHMGQAM 273
           S +WAD+LSRGL++ NIS  K   L D+GV    +       +++   PA    +MG+AM
Sbjct: 715 SSVWADTLSRGLVNFNISGSKTNPLADIGVDFEAINRREKRLEKQTNTPATSTINMGKAM 774

[Close]

BLAST of Lsi08G015630.1 vs. NCBI nr
Match: gi|449450326|ref|XP_004142914.1| (PREDICTED: clathrin interactor EPSIN 1 [Cucumis sativus])

HSP 1 Score: 467.6 bits (1202), Expect = 1.6e-128
Identity = 245/276 (88.77%), Postives = 261/276 (94.57%), Query Frame = 1


Query: 20  VKKKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVS 79
           ++K+  + L+TTEVDLFASQPASVSATVDFF+SP+PVVQT TNFA AARDSKASQPAS S
Sbjct: 346 MEKEPNSPLKTTEVDLFASQPASVSATVDFFSSPDPVVQTGTNFASAARDSKASQPASNS 405

Query: 80  TTIDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVDLFASPEPVVQTETNFASAARD 139
           +TIDFFAS EPVV+TETNFV+AA DIKASQP+SVSAPVDLFASPEPV++TETNFASAARD
Sbjct: 406 STIDFFASSEPVVETETNFVSAARDIKASQPSSVSAPVDLFASPEPVLKTETNFASAARD 465

Query: 140 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAAPAK 199
           SKAVDPFAALPLNSFDGSDL GAPS HSEPV S+PSQ+PVGGPSNNLDGKSINSS APAK
Sbjct: 466 SKAVDPFAALPLNSFDGSDLFGAPSSHSEPVSSQPSQSPVGGPSNNLDGKSINSSIAPAK 525

Query: 200 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMGQ 259
           KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSD SDEREKGPAP YHMGQ
Sbjct: 526 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDFSDEREKGPAPTYHMGQ 585

Query: 260 AMGAGSGLGRTGSQTIGDDFFSQLSGQQYQFGGFQK 296
           AMGAGSGLGRTGSQ +GDDFFSQLSGQQYQFGGFQK
Sbjct: 586 AMGAGSGLGRTGSQALGDDFFSQLSGQQYQFGGFQK 621

[Close]

BLAST of Lsi08G015630.1 vs. NCBI nr
Match: gi|659087529|ref|XP_008444502.1| (PREDICTED: clathrin interactor EPSIN 1 isoform X1 [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 1.3e-125
Identity = 244/276 (88.41%), Postives = 258/276 (93.48%), Query Frame = 1


Query: 20  VKKKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVS 79
           V+K+  + L+TTEVDLFASQPASVSATVDFFASPEPVVQT TNFA AARD+KASQPAS S
Sbjct: 350 VEKEPNSPLKTTEVDLFASQPASVSATVDFFASPEPVVQTGTNFASAARDNKASQPASNS 409

Query: 80  TTIDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVDLFASPEPVVQTETNFASAARD 139
           +TIDFFAS EPVV+TETNFV+AA DIKASQP SVSAPVDLFASPEPV++TETNFASA+RD
Sbjct: 410 STIDFFASSEPVVETETNFVSAARDIKASQPASVSAPVDLFASPEPVLKTETNFASASRD 469

Query: 140 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAAPAK 199
           SKAVDPFAALPLNSFDGSDL GAPS HSEP  S+ SQNPVGGPSNNLDGKSINSS APAK
Sbjct: 470 SKAVDPFAALPLNSFDGSDLFGAPSSHSEP--SQLSQNPVGGPSNNLDGKSINSSIAPAK 529

Query: 200 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMGQ 259
           KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSD SDEREKGPAP Y+MGQ
Sbjct: 530 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDFSDEREKGPAPTYYMGQ 589

Query: 260 AMGAGSGLGRTGSQTIGDDFFSQLSGQQYQFGGFQK 296
           AMGAGSGLGRTGSQ +GDDFFSQLSGQQYQFGGFQK
Sbjct: 590 AMGAGSGLGRTGSQALGDDFFSQLSGQQYQFGGFQK 623

[Close]

BLAST of Lsi08G015630.1 vs. NCBI nr
Match: gi|659087531|ref|XP_008444503.1| (PREDICTED: clathrin interactor EPSIN 1 isoform X2 [Cucumis melo])

HSP 1 Score: 458.0 bits (1177), Expect = 1.3e-125
Identity = 244/276 (88.41%), Postives = 258/276 (93.48%), Query Frame = 1


Query: 20  VKKKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVS 79
           V+K+  + L+TTEVDLFASQPASVSATVDFFASPEPVVQT TNFA AARD+KASQPAS S
Sbjct: 349 VEKEPNSPLKTTEVDLFASQPASVSATVDFFASPEPVVQTGTNFASAARDNKASQPASNS 408

Query: 80  TTIDFFASPEPVVQTETNFVNAASDIKASQPTSVSAPVDLFASPEPVVQTETNFASAARD 139
           +TIDFFAS EPVV+TETNFV+AA DIKASQP SVSAPVDLFASPEPV++TETNFASA+RD
Sbjct: 409 STIDFFASSEPVVETETNFVSAARDIKASQPASVSAPVDLFASPEPVLKTETNFASASRD 468

Query: 140 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINSSAAPAK 199
           SKAVDPFAALPLNSFDGSDL GAPS HSEP  S+ SQNPVGGPSNNLDGKSINSS APAK
Sbjct: 469 SKAVDPFAALPLNSFDGSDLFGAPSSHSEP--SQLSQNPVGGPSNNLDGKSINSSIAPAK 528

Query: 200 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMGQ 259
           KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSD SDEREKGPAP Y+MGQ
Sbjct: 529 KNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDFSDEREKGPAPTYYMGQ 588

Query: 260 AMGAGSGLGRTGSQTIGDDFFSQLSGQQYQFGGFQK 296
           AMGAGSGLGRTGSQ +GDDFFSQLSGQQYQFGGFQK
Sbjct: 589 AMGAGSGLGRTGSQALGDDFFSQLSGQQYQFGGFQK 622

[Close]

BLAST of Lsi08G015630.1 vs. NCBI nr
Match: gi|703101130|ref|XP_010097106.1| (hypothetical protein L484_019545 [Morus notabilis])

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 150/280 (53.57%), Postives = 177/280 (63.21%), Query Frame = 1


Query: 30  TTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPASVSTTIDFFA--- 89
           ++EVDLFA            F S  P V+T  N                 T +D FA   
Sbjct: 323 SSEVDLFADAT---------FVSAAPQVETRANS---------------QTEVDLFADAT 382

Query: 90  --SPEPVVQTETNFVNAAS-DIKASQP---TSVSAPVDLFASPEPVVQTETNFASAAR-D 149
             S  P V+T  +       D+ ASQP   +S S+PVDLFA+P+ V Q E      A  +
Sbjct: 383 FVSAPPQVETRASTQTKNDIDLFASQPPISSSASSPVDLFATPDTVSQPEIKSTHTAPIN 442

Query: 150 SKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVGGPSNNLDGKSINS-SAAPA 209
           +  VDPFAA+PLN+FD SD  GA + HS    SEPSQN V   S+N+ G +I S S + A
Sbjct: 443 ANIVDPFAAVPLNNFDDSDPFGAFTSHSSSASSEPSQNSVNSGSHNVSGGNIASDSKSSA 502

Query: 210 KKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPIYHMG 269
           KK+TFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLS+  DE+EKGPA  Y+MG
Sbjct: 503 KKDTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSEGLDEKEKGPATSYYMG 562

Query: 270 QAMGAGSGLGRTG---SQTIGDDFFSQLSGQQYQFGGFQK 296
           +AMGAGSGLGR+G     T GDDFFS L GQQYQ+GGFQK
Sbjct: 563 RAMGAGSGLGRSGFPSHGTGGDDFFSSLGGQQYQYGGFQK 578

[Close]

BLAST of Lsi08G015630.1 vs. NCBI nr
Match: gi|823225087|ref|XP_012445334.1| (PREDICTED: clathrin interactor EPSIN 1-like [Gossypium raimondii])

HSP 1 Score: 220.3 bits (560), Expect = 4.4e-54
Identity = 143/286 (50.00%), Postives = 178/286 (62.24%), Query Frame = 1


Query: 22  KKSLTFLQTTEVDLFASQPASVSATVDFFASPEPVVQTETNFAGAARDSKASQPA---SV 81
           +KS     +T+VDLFA           F ++P  V    +  A    D  ASQPA   + 
Sbjct: 321 EKSAVSTDSTDVDLFAD--------ATFVSAPTKVTHESSPQAQQQVDLFASQPAITPAA 380

Query: 82  STTIDFFASPEPVVQTETNFVNAASDIKASQPT---SVSAPVDLFASPEPVVQTETNFAS 141
           S T+D FA+ +PVVQ     +    D+ ASQP    +VS  VDLF++ +PVVQ       
Sbjct: 381 SPTVDIFAAGDPVVQPH---IKGQVDLFASQPAITPAVSPTVDLFSTTDPVVQPAMAANV 440

Query: 142 AARDSKAVDPFAALPLNSFDGSDLLGAPSLHSEPVLSEPSQNPVG-GPSNNLDGKSINSS 201
              ++  VDPFAA+PLN+FDGSD+ G+ + HS+  L EP QNP+  G  NN+  KS   S
Sbjct: 441 GPVNANIVDPFAAVPLNNFDGSDIFGSFTTHSDSALEEPKQNPMNDGNLNNMSTKSSQDS 500

Query: 202 AAPAKKNTFQVKSGIWADSLSRGLIDLNISAPKKVSLVDVGVVGGLSDLSDEREKGPAPI 261
            AP KK+ FQVKSGIWADSLSRG+IDLNISAPKKVSL DVG+V GLSD+ DEREKGP   
Sbjct: 501 KAPQKKDAFQVKSGIWADSLSRGIIDLNISAPKKVSLADVGIVCGLSDV-DEREKGPPTS 560

Query: 262 YHMGQAMGAGSGLGRTG----SQTIGDDFFSQLSG-QQYQFGGFQK 296
           ++MG+AMG GSGLG+TG      T  D  FS L   QQYQFG F+K
Sbjct: 561 FYMGRAMGTGSGLGKTGFASTPSTDEDGLFSSLGNHQQYQFGSFKK 594

The following BLAST results are available for this feature:

BLAST of Lsi08G015630.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
EPN1_ARATH	8.4e-37	43.19	Clathrin interactor EPSIN 1 OS=Arabidopsis thaliana GN=EPSIN1 PE=1 SV=1	[more]
EPN3_ARATH	2.8e-08	30.11	Clathrin interactor EPSIN 3 OS=Arabidopsis thaliana GN=EPSIN3 PE=2 SV=1	[more]
EPN2_ARATH	2.8e-08	32.06	Clathrin interactor EPSIN 2 OS=Arabidopsis thaliana GN=EPSIN2 PE=1 SV=1	[more]

BLAST of Lsi08G015630.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKH0_CUCSA	1.1e-128	88.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G352400 PE=4 SV=1	[more]
W9RS28_9ROSA	3.3e-56	53.57	Uncharacterized protein OS=Morus notabilis GN=L484_019545 PE=4 SV=1	[more]
A0A0D2UGC2_GOSRA	3.1e-54	50.00	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1	[more]
A0A0D2TJL0_GOSRA	3.1e-54	50.00	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1	[more]
A0A0D2TDP0_GOSRA	2.6e-53	50.17	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G047500 PE=4 SV=1	[more]

BLAST of Lsi08G015630.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G11710.1	4.7e-38	43.19	ENTH/VHS family protein	[more]
AT3G59290.1	1.6e-09	30.11	ENTH/VHS family protein	[more]
AT2G43160.3	1.6e-09	32.06	ENTH/VHS family protein	[more]

BLAST of Lsi08G015630.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450326\|ref\|XP_004142914.1\|	1.6e-128	88.77	PREDICTED: clathrin interactor EPSIN 1 [Cucumis sativus]	[more]
gi\|659087529\|ref\|XP_008444502.1\|	1.3e-125	88.41	PREDICTED: clathrin interactor EPSIN 1 isoform X1 [Cucumis melo]	[more]
gi\|659087531\|ref\|XP_008444503.1\|	1.3e-125	88.41	PREDICTED: clathrin interactor EPSIN 1 isoform X2 [Cucumis melo]	[more]
gi\|703101130\|ref\|XP_010097106.1\|	4.7e-56	53.57	hypothetical protein L484_019545 [Morus notabilis]	[more]
gi\|823225087\|ref\|XP_012445334.1\|	4.4e-54	50.00	PREDICTED: clathrin interactor EPSIN 1-like [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0030276	clathrin binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006623	protein targeting to vacuole

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006623	protein targeting to vacuole
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0030276	clathrin binding
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi08G015630	Lsi08G015630	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G015630.1.CDS.1	Lsi08G015630.1.CDS.1	CDS
Lsi08G015630.1.CDS.2	Lsi08G015630.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G015630.1.three_prime_UTR.1	Lsi08G015630.1.three_prime_UTR.1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi08G015630.1	Lsi08G015630.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12276	EPSIN/ENT-RELATED	coord: 62..234 score: 2.4E-29coord: 29..37 score: 2.4