Lsi08G014980.1 (mRNA) Bottle gourd (USVL1VR-Ls)

NameLsi08G014980.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCarbohydrate esterase plant-like protein
Locationchr08 : 23132432 .. 23133298 (-)
Sequence length867
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTATTGAGACTATCATTCTTGCTATGTATGATGCTATTTGGCCTTTCCCATTCAGGAGCTGCTTCTATCAAGAACATATTCATCCTCGCTGGTCAGAGTAACATGGCTGGTCGAGGTGGAGTTGAGAAAAATAAACTAGGGAAACTCATGTGGGATAGGCATGTCCCACCACTGTGTCAACCTAACCCATCCATCCTACGATTGAACCCTGCGCGTCGATGGGAGGTAGCACGAGAGCCTCTTCATAGGGGAATCGACCTTAACAAGACAGTCGGGATTGGTCCTGGAATGCCATTTGCCTACCAACTGCGAGCCAAAGCGGGGCCAAAGGCAGGCATTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCTCTTTAATCGAACAATGGAGTAAGAATCCTAGCAATCCTAAAGCAACCTTTTATCGAAATTTCATTAAACGAATTAAAGCATCAGATAAAAAAGGTGGAGTAGTGCGTGCTCTTTTCTGGTTCCAAGGGGAAAGCGATGCAACTATGAATAATACTGCTATGAGATATAAAGACAACCTAAAGAAATTCTTCACTGACATCCGCAATGATATAAAACCTAGATTTTTACCCATCATTGTTGTTAAAATAGCCCTCTATGACTTTATTATGAAGCATGATACTCATAATCTGCCAGTAGTGAGGGCGGCAGAAGATGCAATCAGCAAGGAGCTGCTCGACGTGGTGACCATTGACTCCTGGAAATTACCTATTAACTTTACCACATATGAAGGCTTTAACTTAGATCATGGTCATTTTAATACCAATACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCATTATGGTCATTTACTTTGA

mRNA sequence

ATGACTTTATTGAGACTATCATTCTTGCTATGTATGATGCTATTTGGCCTTTCCCATTCAGGAGCTGCTTCTATCAAGAACATATTCATCCTCGCTGGTCAGAGTAACATGGCTGGTCGAGGTGGAGTTGAGAAAAATAAACTAGGGAAACTCATGTGGGATAGGCATGTCCCACCACTGTGTCAACCTAACCCATCCATCCTACGATTGAACCCTGCGCGTCGATGGGAGGTAGCACGAGAGCCTCTTCATAGGGGAATCGACCTTAACAAGACAGTCGGGATTGGTCCTGGAATGCCATTTGCCTACCAACTGCGAGCCAAAGCGGGGCCAAAGGCAGGCATTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCTCTTTAATCGAACAATGGAGTAAGAATCCTAGCAATCCTAAAGCAACCTTTTATCGAAATTTCATTAAACGAATTAAAGCATCAGATAAAAAAGGTGGAGTAGTGCGTGCTCTTTTCTGGTTCCAAGGGGAAAGCGATGCAACTATGAATAATACTGCTATGAGATATAAAGACAACCTAAAGAAATTCTTCACTGACATCCGCAATGATATAAAACCTAGATTTTTACCCATCATTGTTGTTAAAATAGCCCTCTATGACTTTATTATGAAGCATGATACTCATAATCTGCCAGTAGTGAGGGCGGCAGAAGATGCAATCAGCAAGGAGCTGCTCGACGTGGTGACCATTGACTCCTGGAAATTACCTATTAACTTTACCACATATGAAGGCTTTAACTTAGATCATGGTCATTTTAATACCAATACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCATTATGGTCATTTACTTTGA

Coding sequence (CDS)

ATGACTTTATTGAGACTATCATTCTTGCTATGTATGATGCTATTTGGCCTTTCCCATTCAGGAGCTGCTTCTATCAAGAACATATTCATCCTCGCTGGTCAGAGTAACATGGCTGGTCGAGGTGGAGTTGAGAAAAATAAACTAGGGAAACTCATGTGGGATAGGCATGTCCCACCACTGTGTCAACCTAACCCATCCATCCTACGATTGAACCCTGCGCGTCGATGGGAGGTAGCACGAGAGCCTCTTCATAGGGGAATCGACCTTAACAAGACAGTCGGGATTGGTCCTGGAATGCCATTTGCCTACCAACTGCGAGCCAAAGCGGGGCCAAAGGCAGGCATTGTGGGTTTAGTTCCTTGTGCTAGAGGTGGCTCTTTAATCGAACAATGGAGTAAGAATCCTAGCAATCCTAAAGCAACCTTTTATCGAAATTTCATTAAACGAATTAAAGCATCAGATAAAAAAGGTGGAGTAGTGCGTGCTCTTTTCTGGTTCCAAGGGGAAAGCGATGCAACTATGAATAATACTGCTATGAGATATAAAGACAACCTAAAGAAATTCTTCACTGACATCCGCAATGATATAAAACCTAGATTTTTACCCATCATTGTTGTTAAAATAGCCCTCTATGACTTTATTATGAAGCATGATACTCATAATCTGCCAGTAGTGAGGGCGGCAGAAGATGCAATCAGCAAGGAGCTGCTCGACGTGGTGACCATTGACTCCTGGAAATTACCTATTAACTTTACCACATATGAAGGCTTTAACTTAGATCATGGTCATTTTAATACCAATACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCATTATGGTCATTTACTTTGA

Protein sequence

MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPLCQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL
BLAST of Lsi08G014980.1 vs. Swiss-Prot
Match: CAES_ARATH (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 9.0e-44
Identity = 99/258 (38.37%), Postives = 146/258 (56.59%), Query Frame = 1

Query: 28  IFILAGQSNMAGRGGVEKNKLG-KLMWDRHVPPLCQPNPSILRLNPARRWEVAREPLHRG 87
           IFIL+GQSNMAGRGGV K+    + +WD+ +PP C PN SILRL+   RWE A EPLH  
Sbjct: 24  IFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVD 83

Query: 88  IDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNF 147
           ID  K  G+GPGM FA  ++ +    + ++GLVPCA GG+ I++W +      +  Y   
Sbjct: 84  IDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERG-----SHLYERM 143

Query: 148 IKRIKASDKKGGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVV 207
           +KR + S K GG ++A+ W+QGESD    + A  Y +N+ +   ++R+D+    LPII V
Sbjct: 144 VKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQV 203

Query: 208 KIALYDFIMKHDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNT 267
            IA         +    + +  E  +  +L +VV +D+  LP+          D+ H  T
Sbjct: 204 AIA---------SGGGYIDKVREAQLGLKLSNVVCVDAKGLPL--------KSDNLHLTT 259

Query: 268 NTEIALGKWLADTYLSHY 285
             ++ LG  LA  YLS++
Sbjct: 264 EAQVQLGLSLAQAYLSNF 259

BLAST of Lsi08G014980.1 vs. TrEMBL
Match: A0A0A0LNC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356040 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.1e-128
Identity = 225/288 (78.12%), Postives = 248/288 (86.11%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPL 60
           M LLRLS +LC+ML+G S SGAAS KNIFILAGQSNMAGRGGVE N  G L WD  VPP 
Sbjct: 1   MVLLRLSIILCVMLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPE 60

Query: 61  CQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVP 120
           CQP PSILRLNP  +WE+AREPLH GID+ +T GIGPG+ FA++L  KAGP AG VGLVP
Sbjct: 61  CQPQPSILRLNPGLQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVP 120

Query: 121 CARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMR 180
           CARGG+LIEQW KNPSNP ATFY+NFI+RIKASDK GGVVRALFWFQGESDA MN+TA+R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIR 180

Query: 181 YKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVV 240
           YKDNLKKFFTDIR+DIKPRFLPIIVVKIALYDF  +HDTHNLP VR A++A+SKEL DVV
Sbjct: 181 YKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVV 240

Query: 241 TIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL 289
            IDS KLPIN+TT EG NLDHGHFNT TEI LGKWLA+TYLSH+G LL
Sbjct: 241 AIDSLKLPINYTTNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

BLAST of Lsi08G014980.1 vs. TrEMBL
Match: E5GB85_CUCME (Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.2e-106
Identity = 191/288 (66.32%), Postives = 224/288 (77.78%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPL 60
           M L++LS LLC MLFG S SGA S +NIFIL GQSNMAGRGGVEKN  GK  WD  +PP 
Sbjct: 4   MALVKLSILLCAMLFGPSLSGAVSPQNIFILGGQSNMAGRGGVEKNSSGKFEWDGVIPPD 63

Query: 61  CQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVP 120
           C+PNPSILRLN AR+WEVAREPLH  ID+ K  GI PGM FA++L  KAGP+AG+VGLVP
Sbjct: 64  CKPNPSILRLNAARQWEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVP 123

Query: 121 CARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMR 180
            A GG+ I QW KN S P AT+Y+N ++RI+ASDK+GGVVRAL WFQGESDA +   A+ 
Sbjct: 124 TAIGGTFIRQWLKNDSYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAIN 183

Query: 181 YKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVV 240
           YKDNLK F  D+R DI+PRFLP+I+VKIALYDF+  + T NL +VRAA++A+SKE+ DV 
Sbjct: 184 YKDNLKTFIMDLRRDIQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKEVPDVS 243

Query: 241 TIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL 289
            IDSWKLP+N  T EGFNLD GHFNT  E+  GKWLAD YLS Y  LL
Sbjct: 244 IIDSWKLPMNLKTREGFNLDRGHFNTTIELTAGKWLADAYLSRYSRLL 291

BLAST of Lsi08G014980.1 vs. TrEMBL
Match: A0A0A0KAL7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G135410 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-89
Identity = 159/190 (83.68%), Postives = 173/190 (91.05%), Query Frame = 1

Query: 99  MPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGG 158
           MPFA++L AK GP AG VGLVPCARGG+LIE+W KNPSNP ATFY+NFI+RIKASDK GG
Sbjct: 1   MPFAHELLAKVGPNAGAVGLVPCARGGTLIEEWVKNPSNPSATFYQNFIERIKASDKDGG 60

Query: 159 VVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHD 218
           VVRALFWFQGESDA MN+TA+RYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDF+M+HD
Sbjct: 61  VVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFMMQHD 120

Query: 219 THNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLAD 278
           THNLP VR A+DA+SKEL DVV IDS +LPIN TT EGFNLDHGHFNT TEI LGKWLA+
Sbjct: 121 THNLPAVREAQDAVSKELPDVVAIDSLELPINLTTNEGFNLDHGHFNTTTEITLGKWLAN 180

Query: 279 TYLSHYGHLL 289
           TYLSHYGHLL
Sbjct: 181 TYLSHYGHLL 190

BLAST of Lsi08G014980.1 vs. TrEMBL
Match: A0A0A0LKQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356050 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.4e-67
Identity = 121/195 (62.05%), Postives = 152/195 (77.95%), Query Frame = 1

Query: 94  GIGPGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKAS 153
           GI PGM FA+++  KAGP+AG+VGLVP A GG++I QW KN ++P AT+Y++ ++RIKAS
Sbjct: 32  GISPGMGFAHEILRKAGPRAGVVGLVPTAIGGTVIRQWMKNTTDPNATYYQHLVERIKAS 91

Query: 154 DKKGGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDF 213
           DK GGVVRAL WFQGESDA + + A+ YKDNLK    D+RND+KPRFLP+I+VKIA+YDF
Sbjct: 92  DKDGGVVRALLWFQGESDAAVKDYAINYKDNLKTLINDLRNDLKPRFLPVILVKIAIYDF 151

Query: 214 IMKHDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALG 273
              + T NL  VRAA++A+S E+ DV  IDSWKLP+N TT EGFNLD GHFN+   +  G
Sbjct: 152 FAVNGTDNLSTVRAAQEAVSNEVPDVSIIDSWKLPMNLTTREGFNLDRGHFNSTVLLTAG 211

Query: 274 KWLADTYLSHYGHLL 289
           +WLADTYLS Y  LL
Sbjct: 212 RWLADTYLSRYSQLL 226

BLAST of Lsi08G014980.1 vs. TrEMBL
Match: A0A0A0K9J9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G058180 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.2e-56
Identity = 121/271 (44.65%), Postives = 162/271 (59.78%), Query Frame = 1

Query: 17  LSHSGAASIKNIFILAGQSNMAGRGGVEKNKL-GKLMWDRHVPPLCQPNPSILRLNPARR 76
           LS     S  NIFILAGQSNMAGRGGV  +    K++WD ++P  C+ N SI RLN    
Sbjct: 6   LSPKATTSPNNIFILAGQSNMAGRGGVSLDPTTDKMVWDGYIPLECESNDSIFRLNADMV 65

Query: 77  WEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNP 136
           WE A EPLH  ID+ KT GIGPGM FA +L A  G + G +GLVPCA GGS +++W K  
Sbjct: 66  WEQAHEPLHWDIDVVKTNGIGPGMAFANELLAIGGKRIGAIGLVPCAIGGSHLKEWVKGT 125

Query: 137 SNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRND 196
           +      Y N ++RI+AS+K GG V+ + W+QGESDA +   AM Y+  L KFF D+R D
Sbjct: 126 NR-----YDNLVERIRASEKNGGTVQGILWYQGESDAAVEEEAMCYERELTKFFIDLRAD 185

Query: 197 IKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYE 256
                LPII+VK+  +DF +  +      V  A +A++  L +V  +D      NF   +
Sbjct: 186 TNHPELPIILVKLVTHDFFLSPNISFKEEVCNALEAVTHRLPNVTMVDGPMAVGNFD--D 245

Query: 257 GFNLDHGHFNTNTEIALGKWLADTYLSHYGH 287
           G N D GH N  +E+ LGK  A ++ S++ H
Sbjct: 246 GLNEDKGHLNVKSEVKLGKMFAHSFYSNFAH 269

BLAST of Lsi08G014980.1 vs. TAIR10
Match: AT4G34215.1 (AT4G34215.1 Domain of unknown function (DUF303) )

HSP 1 Score: 178.7 bits (452), Expect = 5.1e-45
Identity = 99/258 (38.37%), Postives = 146/258 (56.59%), Query Frame = 1

Query: 28  IFILAGQSNMAGRGGVEKNKLG-KLMWDRHVPPLCQPNPSILRLNPARRWEVAREPLHRG 87
           IFIL+GQSNMAGRGGV K+    + +WD+ +PP C PN SILRL+   RWE A EPLH  
Sbjct: 24  IFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPLHVD 83

Query: 88  IDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNF 147
           ID  K  G+GPGM FA  ++ +    + ++GLVPCA GG+ I++W +      +  Y   
Sbjct: 84  IDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERG-----SHLYERM 143

Query: 148 IKRIKASDKKGGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVV 207
           +KR + S K GG ++A+ W+QGESD    + A  Y +N+ +   ++R+D+    LPII V
Sbjct: 144 VKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPIIQV 203

Query: 208 KIALYDFIMKHDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNT 267
            IA         +    + +  E  +  +L +VV +D+  LP+          D+ H  T
Sbjct: 204 AIA---------SGGGYIDKVREAQLGLKLSNVVCVDAKGLPL--------KSDNLHLTT 259

Query: 268 NTEIALGKWLADTYLSHY 285
             ++ LG  LA  YLS++
Sbjct: 264 EAQVQLGLSLAQAYLSNF 259

BLAST of Lsi08G014980.1 vs. TAIR10
Match: AT3G53010.1 (AT3G53010.1 Domain of unknown function (DUF303) )

HSP 1 Score: 173.3 bits (438), Expect = 2.1e-43
Identity = 113/289 (39.10%), Postives = 158/289 (54.67%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIK---NIFILAGQSNMAGRGGVEKNKL-GKLMWDRH 60
           MT +   F + + L    H  + +I    +IFILAGQSNMAGRGGV  +      +WD  
Sbjct: 1   MTKIFYYFFIIIFLQSPPHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGV 60

Query: 61  VPPLCQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIV 120
           +PP C+ NPSILRL     W+ A+EPLH  ID+NKT G+GPGMPFA ++  + G     V
Sbjct: 61  IPPECRSNPSILRLTSKLEWKEAKEPLHVDIDINKTNGVGPGMPFANRVVNRFGQ----V 120

Query: 121 GLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKA--SDKKGGVVRALFWFQGESDATM 180
           GLVPC+ GG+ + QW K         Y   +KR KA  +   GG  RA+ W+QGESD   
Sbjct: 121 GLVPCSIGGTKLSQWQKG-----EFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVD 180

Query: 181 NNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDA-IS 240
              A  YK  L KFF+D+RND++   LPII V +A         T   P + A   A + 
Sbjct: 181 MVDASVYKKRLVKFFSDLRNDLQHPNLPIIQVALA---------TGAGPYLDAVRKAQLK 240

Query: 241 KELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLS 283
            +L +V  +D+  LP+          D  H  T++++ LG  +A+++L+
Sbjct: 241 TDLENVYCVDARGLPL--------EPDGLHLTTSSQVQLGHMIAESFLA 263

BLAST of Lsi08G014980.1 vs. NCBI nr
Match: gi|778671052|ref|XP_011649575.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus])

HSP 1 Score: 465.7 bits (1197), Expect = 5.9e-128
Identity = 225/288 (78.12%), Postives = 248/288 (86.11%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPL 60
           M LLRLS +LC+ML+G S SGAAS KNIFILAGQSNMAGRGGVE N  G L WD  VPP 
Sbjct: 1   MVLLRLSIILCVMLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPE 60

Query: 61  CQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVP 120
           CQP PSILRLNP  +WE+AREPLH GID+ +T GIGPG+ FA++L  KAGP AG VGLVP
Sbjct: 61  CQPQPSILRLNPGLQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVP 120

Query: 121 CARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMR 180
           CARGG+LIEQW KNPSNP ATFY+NFI+RIKASDK GGVVRALFWFQGESDA MN+TA+R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIR 180

Query: 181 YKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVV 240
           YKDNLKKFFTDIR+DIKPRFLPIIVVKIALYDF  +HDTHNLP VR A++A+SKEL DVV
Sbjct: 181 YKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVV 240

Query: 241 TIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL 289
            IDS KLPIN+TT EG NLDHGHFNT TEI LGKWLA+TYLSH+G LL
Sbjct: 241 AIDSLKLPINYTTNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

BLAST of Lsi08G014980.1 vs. NCBI nr
Match: gi|778722113|ref|XP_011658406.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus])

HSP 1 Score: 436.8 bits (1122), Expect = 2.9e-119
Identity = 205/252 (81.35%), Postives = 223/252 (88.49%), Query Frame = 1

Query: 37  MAGRGGVEKNKLGKLMWDRHVPPLCQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIG 96
           MAGRGGVE N  G LMWD  VPP CQ  PSILRLNP R+WE+AREPLH GID+N+T GIG
Sbjct: 1   MAGRGGVENNNKGNLMWDGLVPPECQSEPSILRLNPDRQWEIAREPLHLGIDINRTPGIG 60

Query: 97  PGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKK 156
           PGMPFA++L AK GP AG VGLVPCARGG+LIE+W KNPSNP ATFY+NFI+RIKASDK 
Sbjct: 61  PGMPFAHELLAKVGPNAGAVGLVPCARGGTLIEEWVKNPSNPSATFYQNFIERIKASDKD 120

Query: 157 GGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMK 216
           GGVVRALFWFQGESDA MN+TA+RYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDF+M+
Sbjct: 121 GGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFMMQ 180

Query: 217 HDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWL 276
           HDTHNLP VR A+DA+SKEL DVV IDS +LPIN TT EGFNLDHGHFNT TEI LGKWL
Sbjct: 181 HDTHNLPAVREAQDAVSKELPDVVAIDSLELPINLTTNEGFNLDHGHFNTTTEITLGKWL 240

Query: 277 ADTYLSHYGHLL 289
           A+TYLSHYGHLL
Sbjct: 241 ANTYLSHYGHLL 252

BLAST of Lsi08G014980.1 vs. NCBI nr
Match: gi|659133975|ref|XP_008466975.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo])

HSP 1 Score: 424.1 bits (1089), Expect = 2.0e-115
Identity = 196/252 (77.78%), Postives = 221/252 (87.70%), Query Frame = 1

Query: 37  MAGRGGVEKNKLGKLMWDRHVPPLCQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIG 96
           MAGRGGVEK++ G L+WDR VPP C+P PSILRLNP R WE AREPLH GID+N+T GIG
Sbjct: 1   MAGRGGVEKDQSGNLVWDRLVPPECEPQPSILRLNPEREWETAREPLHVGIDINRTAGIG 60

Query: 97  PGMPFAYQLRAKAGPKAGIVGLVPCARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKK 156
           PGMPFA+ L AKAGP AG+VGLVPCARGG+LIEQW KNPSNP ATFY+NFI+RIKASDK 
Sbjct: 61  PGMPFAHHLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPSNPNATFYKNFIERIKASDKD 120

Query: 157 GGVVRALFWFQGESDATMNNTAMRYKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMK 216
           GGVVRALFWFQGESDA M++TA RYKDNLK+FFTDIRNDIKPRFLPII+ KIA+YD  MK
Sbjct: 121 GGVVRALFWFQGESDAAMSDTAHRYKDNLKQFFTDIRNDIKPRFLPIILAKIAVYDPFMK 180

Query: 217 HDTHNLPVVRAAEDAISKELLDVVTIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWL 276
           HDTH+L  VRAA++ +SKEL D++TID+ +LPINFTT  GFNLDH HFNTNTEI +GKW 
Sbjct: 181 HDTHDLAAVRAAQEEVSKELPDILTIDALQLPINFTTNAGFNLDHAHFNTNTEIVVGKWF 240

Query: 277 ADTYLSHYGHLL 289
           ADTYLSHYGHLL
Sbjct: 241 ADTYLSHYGHLL 252

BLAST of Lsi08G014980.1 vs. NCBI nr
Match: gi|659087703|ref|XP_008444593.1| (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 1.3e-111
Identity = 199/288 (69.10%), Postives = 234/288 (81.25%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPL 60
           M L++LS LLC MLFG S SGA S +NIFIL GQSNMAGRGGVEKN  GK  WD  +PP 
Sbjct: 4   MALVKLSILLCAMLFGPSLSGAVSPQNIFILGGQSNMAGRGGVEKNSSGKFEWDGVIPPD 63

Query: 61  CQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVP 120
           C+PNPSILRLN AR+WEVAREPLH  ID+ K  GI PGM FA++L  KAGP+AG+VGLVP
Sbjct: 64  CKPNPSILRLNAARQWEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVP 123

Query: 121 CARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMR 180
            A GG+ I QW KN S P AT+Y+N ++RI+ASDK+GGVVRAL WFQGESDA +   A+ 
Sbjct: 124 TAIGGTFIRQWLKNDSYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAIN 183

Query: 181 YKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVV 240
           YKDNLK F  D+R DI+PRFLP+I+VKIALYDF+  + T NL +VRAA++A+SKEL DVV
Sbjct: 184 YKDNLKTFIMDLRRDIQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKELPDVV 243

Query: 241 TIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL 289
           TID+ +LPINFTT EG NLDHGHFNT+TEI LGKWLA+TYLSH+GHLL
Sbjct: 244 TIDALELPINFTTNEGLNLDHGHFNTSTEITLGKWLANTYLSHFGHLL 291

BLAST of Lsi08G014980.1 vs. NCBI nr
Match: gi|307135858|gb|ADN33727.1| (hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 394.4 bits (1012), Expect = 1.7e-106
Identity = 191/288 (66.32%), Postives = 224/288 (77.78%), Query Frame = 1

Query: 1   MTLLRLSFLLCMMLFGLSHSGAASIKNIFILAGQSNMAGRGGVEKNKLGKLMWDRHVPPL 60
           M L++LS LLC MLFG S SGA S +NIFIL GQSNMAGRGGVEKN  GK  WD  +PP 
Sbjct: 4   MALVKLSILLCAMLFGPSLSGAVSPQNIFILGGQSNMAGRGGVEKNSSGKFEWDGVIPPD 63

Query: 61  CQPNPSILRLNPARRWEVAREPLHRGIDLNKTVGIGPGMPFAYQLRAKAGPKAGIVGLVP 120
           C+PNPSILRLN AR+WEVAREPLH  ID+ K  GI PGM FA++L  KAGP+AG+VGLVP
Sbjct: 64  CKPNPSILRLNAARQWEVAREPLHWDIDVMKANGISPGMGFAHELLVKAGPRAGVVGLVP 123

Query: 121 CARGGSLIEQWSKNPSNPKATFYRNFIKRIKASDKKGGVVRALFWFQGESDATMNNTAMR 180
            A GG+ I QW KN S P AT+Y+N ++RI+ASDK+GGVVRAL WFQGESDA +   A+ 
Sbjct: 124 TAIGGTFIRQWLKNDSYPNATYYQNLVERIQASDKEGGVVRALLWFQGESDAAVKEEAIN 183

Query: 181 YKDNLKKFFTDIRNDIKPRFLPIIVVKIALYDFIMKHDTHNLPVVRAAEDAISKELLDVV 240
           YKDNLK F  D+R DI+PRFLP+I+VKIALYDF+  + T NL +VRAA++A+SKE+ DV 
Sbjct: 184 YKDNLKTFIMDLRRDIQPRFLPVIIVKIALYDFLRANATDNLSIVRAAQEAVSKEVPDVS 243

Query: 241 TIDSWKLPINFTTYEGFNLDHGHFNTNTEIALGKWLADTYLSHYGHLL 289
            IDSWKLP+N  T EGFNLD GHFNT  E+  GKWLAD YLS Y  LL
Sbjct: 244 IIDSWKLPMNLKTREGFNLDRGHFNTTIELTAGKWLADAYLSRYSRLL 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CAES_ARATH9.0e-4438.37Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana GN=At4g34215 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0LNC5_CUCSA4.1e-12878.13Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356040 PE=4 SV=1[more]
E5GB85_CUCME1.2e-10666.32Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0KAL7_CUCSA1.7e-8983.68Uncharacterized protein OS=Cucumis sativus GN=Csa_6G135410 PE=4 SV=1[more]
A0A0A0LKQ6_CUCSA2.4e-6762.05Uncharacterized protein OS=Cucumis sativus GN=Csa_2G356050 PE=4 SV=1[more]
A0A0A0K9J9_CUCSA7.2e-5644.65Uncharacterized protein OS=Cucumis sativus GN=Csa_6G058180 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34215.15.1e-4538.37 Domain of unknown function (DUF303) [more]
AT3G53010.12.1e-4339.10 Domain of unknown function (DUF303) [more]
Match NameE-valueIdentityDescription
gi|778671052|ref|XP_011649575.1|5.9e-12878.13PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus][more]
gi|778722113|ref|XP_011658406.1|2.9e-11981.35PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis sativus][more]
gi|659133975|ref|XP_008466975.1|2.0e-11577.78PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo][more]
gi|659087703|ref|XP_008444593.1|1.3e-11169.10PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo][more]
gi|307135858|gb|ADN33727.1|1.7e-10666.32hypothetical protein [Cucumis melo subsp. melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005181SASA
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi08G014980Lsi08G014980gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi08G014980.1.CDS.1Lsi08G014980.1.CDS.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi08G014980.1Lsi08G014980.1-proteinpolypeptide


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005181Sialate O-acetylesterase domainPFAMPF03629SASAcoord: 160..236
score: 5.
NoneNo IPR availablePANTHERPTHR31988FAMILY NOT NAMEDcoord: 26..282
score: 6.5
NoneNo IPR availablePANTHERPTHR31988:SF4SUBFAMILY NOT NAMEDcoord: 26..282
score: 6.5