Lsi08G009290.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G009290.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	RNA pseudouridine synthase
Location	chr08 : 17691076 .. 17697187 (-)
Sequence length	2430

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTTCTTCACGCCGTCTTCTTTCTTCCGCTCGTGCTGCCGCTGCCGTCGTCTTTCTTCCGTTCGCGCCGCCGCAGCCTCCCTCCTGGCGGTTTTGCACTGATTTTCTACTCCCTTGTTGTTACTGGTATTCTTCCAATCTCCATCTATATATTTTCTCACTTAAAAAATACAAATCAGAACTTGTCTAATGAAACAATGGTTTAGGATTTGTTTGTTTGTTTTAGTTTTGTTAAATTCAACTACTTGATTTTTGAAATTGAGACAGAAGGGTACGATGAAGCAATAAATTTGGCATTTTCCTTACTTTGAAGTTTGATATTGACCGATAAAGTGAGAAAAAAAGTTGGACAAAGGGGGAGGGGAGTGAGTTCTGAAGTTGCATCTTCTTCCATAACGAGTGAAACTGGAGATGTTGGGCATCCATACTTGATTTAATTCATAGCTTGAACTTGAAGGCAAGCAGACGTCCCACCTCTCGTCTCCCCCTTCCTTTCGTCTAGTACGGACTCTCACGTCTTCAATCGGTAGTGAAATAGGAGGGATCAGCCAAAGGGGCACCTAATTTTGTTTACATTTTCCCCCTTGCAATAACGGGCGGAAGTGAGCTGCTAGCATCTTCTTCGCTGGGATTCTCTCAATCTCTGAGCATCCACGGAGGTAATTTGTGAGTTGTTTTTCGTCTATGCTATTTGTTGAGGATCCCACATTGAAAAAATTAAGGGACTTTACACTCTTAATAAGTTAGACGAGTTACTCCTCTCCTTGCCAATTGGTTTTGAGATGGACTCCATACTATTTAATACTAATATACTATTGATGTGTGTGTGTATAATTTCATTTTTTTGGTTACTTCCAGCATCATTTCTTCCATCTATTAAAATGAAAATTTCCCACTTTAGTACCATAAATTTTGGTATCGGCATTCCATAAATTTTGAGGTTGGCGTTGATAACTGAGTAGTGAGAGGGAGAGAGCTTCTCCATTGTTGTGTCCCCCCTAAGATTCACCTTCTGTCTCACCCAGGACCCATCCATCAATTTTAAGATCAAACAACTAGCTAGTTGGTCCTCTTCTGTGCCATTCTAATTTTATGTAATTGATTGCATGGCCTGCATTATCAGATTTGCCACTCTTTCTTAGTCATGGAAATGTTAATTGTCTTACTTATTTGTCCGTTTTGCTTAATGCTATTGTGCTTTACAAAGGACTGTGCTAAGCTTGATAGAAATTTAGCATATAAAAAAGAAGTAAAGCTGGGAATCAGGCTTATTAGAGTATTTCATTTTTGTAATGAGTAGAGACTGAATGAAATTCTCCGAGGTTGAAAATACGGGGCACTCAATTGCACAGTTGAAACTAACACACTTGTACACAGGGGAAATCGATAAAGCTACAAATAATAGAAATATATTTTGAGCATCTTTCTTTCCAATCAAAACCACATATTATTTATTTGCCAGTCAACCTATTTGCTTTGTAGGCATTTTCAGAAAGTGCATGGGGAGGGGTGCATTTCTATTTTGTATAAAGAACAAAGAAAACCCCTACTTATAATTGCGCCTGTAGCCATCAGGGGCTGTTTTGCTTGAAAGATTACAGGATTATGCAGCAGAACATACGACGGCATCTTTTACTTGCAACCAGATACTACTCTAGAATTTCTCCTCCACCACCTGCCTATGCCAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAAGGCCCAAAGGCCCGGCAGCTCCTATCCCTGCCCCCATTTCCTGGTTACCCTTTGCCAGGAAAGAATCAGATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCGTAAGATGGGTCAAGCATTACTTTGATGAAATTCAAGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTAAGTACGAACACTTACTCCTCTTGTGCTTTGCTTATATCTAGTCTTGTAGGCTTCATGTAACTTTTGTCATGACGAGAACCTCTTTTCATCTTTTTGAGCAGTGAGACCAGTGCTAACTTGTAAGTTGTAAATAGTAATGGGAGCTTAATCTCTTCTTGCTTGTTGAAAGAAATTGAGTTCCATACTTTTCTTGAGTTATTAGTATAAATAAACTTAAATTTCTTCTGTGGGAAAAAAGTGCATTGGACTCGCTTTGTTTCAGAATTTCAGTTGTGGGGGGGGGTTGGAATTTTTGGTTATCTAATCAATTCTGGGTTTAAATTTATCTTCCTTCTTCCAGGTCCAGGTGGAATGCCCACAATTGGGTGGGTTGGATGAAAAAGGAAATTTGAAACCTATGAGAAAGGTACTGTTTCTTGAATATATAGCCTATTCTCTTATGATTCATTTGGAATTTCAATGAGGACATTTTTGAGAAGCTTTTGATCTGTTGTTTATGCGTACATAATATCTGGTTTCATTTACTTATTGAAATCATTCTTTGTTGGCTGTAAGACTATTAACTTATTATTAGATGAATTTCAGATTAGGAGTAGTGAGATTATGGAAACTGGGGCACGCATCCATATACCTGTGTCAGTTGCAGAGATGAAGGTTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAGAGACTTGTCAAGTACAAGGCAAGTATTGAGTTTAACTTGCATTCTCCTCTTTGCTGTTTGCATCAATTAATGAATCTCCCAGCGTTTTATCCATTGATATACAAGCAGTTGCTATATACTTGGTATGGAAAGATCCACATAGGTGTACTCATTTTCTTATGTACTATCAAATACATGAGTTGAGATTGATGGTTAATTAATTTTCTTCTCTCCATTGCTTCATTTTACCCTGGCATATGATTTTGTTGTATGCTAAGCGTGATTTTATTTGTTTCTTAATTTTGAAAATTTCAGGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCAGTCAAGGTTTACCTCTTATTCTCTTTTACTTTTCTATCTTATTTCTGGTGGTGAGTGCCGGGTGGGCAGGTGGGGTTGGAGGTAATAATAAGTGCTCTGATATTTTGAAACAATTATGGTATTGTCAGGGGAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCAGCTGCAGCATTATCTTATGACTACGATGATGGACCCAAGTTGGTAAGCGTCTTGTTTGCGTCTCAGTAACTTATGTAGATATTGTTATTAAAAGACTTTGTATCGAGGATGAAACTTAACTACTGTTTGATAATCATTTGGTTTTTAGTTTTCGGTTTTTGAGCTTATAAACATTACTTCCACTTATTGATTTTTTTTGTTTTGTTATCTTTTTAGGACTGTTTTAAAAACTCAAGCTAAGGTTTGAAAACTACCCAAAAAAAAAAAGTAGTTCTTATAAACTTGTTCTTGTTTTTAGAATTTGACTAAGAATTTAAATTTTTAGTTAAGAAAAATGAAAACCATGGTAAAGAAGTTATAAGAAAACAAACATAATTTGGTGGACACTTATGGGGATAAGATGGGCTGTGGCGCATACACAGCCATGCGGTTGTGTGGTGATTCAATTGGAAGATTTGGTAATGTAGGATAAAAGTATTTGCTTTAAGTTGTAGCAATTTCGAGGCATTTGTCTCATAGACATGGGATAAATTTGGATGGATAAAGCTCCTTTGTATTTGTAGTGGGTTTTTCTTCTCTTTGTATAGCCCAAAGCTTTGGCTTTCATTGAAAACCTAATCCCAACTGAAAGAAGTTATATAGAAATTAAGTTTCCTTCATTTCAAGAAATTGTGTTTATTTCTTCAAAATAAGGACGTAGAAGCTACTATATTTTCTTACTTTTCATCATGTGGCATCTTTGGTTGCCTTGTTCTATACTTAATGCTCTTTCTCTATCTATTCTTATGGATGCACATTTAGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAGGAAAGTGTAGCCCACCTGCAATGGCTATTTGGCAATATAAGAAATGCGAAATCTACATGTAAGGTGCTTAATTAAAAAATGCCAAAAATGCATTTATCTTCAAGATGTCATTTGTCAATTATCTAGTAACTATGGCTAAGAAATGTTCTAGTATTTCTGATTGTTCAAACTTTAGCGACAACGATTTTAGGCTTTGGACGAGGCATGTAATGCAACATATCAAAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATCTCTGCTCCTCTGTCAAAGGTTCTCTCTCTATTTCTGTCTCTAGGAACAGTATTCATGATTCTCAGTCTTTTCCTGATAAGTTGATAATTATAATACAATCATGTACCAGAGGTGCAGTGGTTGGCAGTTGGTTTATGTGCGGTTTTTGTTACTCTGCCTATAGAACTCGAGAATATGGGTCAAACAAGTTTCTTGAGGATCAGTTCATGTTGCTTTAATTGTGCTATAATTAATTGTAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCAAACAAAACATCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATAAATGGTTGTTCATGGATTGAACTATGTCCACGTACTAGCCGAAAACATCAGGTACTTCAAGCTCTCTCTCCCCCCTATATGCACAGAAAACATTCAAATTTATGCTTTCCCTTTTTGGTTCCTAGATTTTGAGTTTAGTTTTCATTTGGTTCCTAGATTTTAAAATATTACACTTTTACTGTTGAGCTTTGAGTTAATATTTTCATTTGGTTCTTAGATTTCATTTCAAAATGTTATAATTTTACCCTTGAGTTTTAAGTTTAGATTTCATTTGGTCCTTAGGTTTCAATATTTTACCCTTTTACTCTCAAGTTTTCACTCATGTTTACTTTTGACCTTTGACATCAATTTTTATTTACAATAATTATGATGTGAATTTTCTTAAAACTAATTTTAATAGTGATGGAAAATTAGTGAAAACTAATATAATATAGTTATATTTAATTTAATCATTTTAAATTAATAAATATGCATTAACACCAAGGATGGAAGGTAAGTACTTGTTGAAAATCTAAGGGTAAAAGTGTGAATTTTGAAGCCTAGAGACCAAATGAAAACACTCAAAACTCATGGATAAAAATGTAACATTTTGGAATGTAGGCGCCAAATGGAAATCAAACTAAAAAATTCGATAGTAAAGTGTAACATTTTGAAATTTAAAAATCAAAAGGAAACTACACTCAAAATTTAGGGACCAACAAATTTTTTTTTTTTCCCCCGAAATCCTAACTGTATGATCTTTAGTACTGATCCTGTAAACCATCAAGGAAAGTGTTAATCTTTTTTCATCTAATCTGATTTTTTATGGAGGTAAATGTTCCTTCCTATTCTATAGTAACTTCGTTTTGTACATATCCACTACCATCTTTCAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACACCCATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGAACGTGGAAACAAATGCCCCGAGTCGATGTTGAGCCGATAAGTGGGAGGCCATACAAGTTGCGAAGGCCAGAAGGGTTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCCTTATTACATCTTCACTGCAGAGAGCTCGTTCTTCCCAATTTTGCTAAGTTCCTTAATGTTTTGGATGATAAAAGATCGATCAGCCACCAGCCGGTATCTAGCTTGAACTCTGATCTCTTGCGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGAGTATCTTGAGTTTTCATAGGTGGATTTCATGCAACTAAAGGGAAATAAACATGATTGTAATAGGATTATCTATTTATTAAATTATTATTAGTCTTAGCCTTTTAGGTTAAACTATATGTTTACTCACTTAACGTTAAAAGTGTCGAATAAGTCTCTAAACTTTCAATTTTGTATCAAATCGATCATTGAATTTTAAGAAACGTGTAATAGGTTCTTGACCTTTCATTATTGTGTC

mRNA sequence

TCTTCTTCTTCACGCCGTCTTCTTTCTTCCGCTCGTGCTGCCGCTGCCGTCGTCTTTCTTCCGTTCGCGCCGCCGCAGCCTCCCTCCTGGCGGTTTTGCACTGATTTTCTACTCCCTTGTTGTTACTGACAGAAGGGTACGATGAAGCAATAAATTTGGCATTTTCCTTACTTTGAAGTTTGATATTGACCGATAAAGTGAGAAAAAAAGTTGGACAAAGGGGGAGGGGAGTGAGTTCTGAAGTTGCATCTTCTTCCATAACGAGTGAAACTGGAGATGTTGGGCATCCATACTTGATTTAATTCATAGCTTGAACTTGAAGGCAAGCAGACGTCCCACCTCTCGTCTCCCCCTTCCTTTCGTCTAGTACGGACTCTCACGTCTTCAATCGGTAGTGAAATAGGAGGGATCAGCCAAAGGGGCACCTAATTTTGTTTACATTTTCCCCCTTGCAATAACGGGCGGAAGTGAGCTGCTAGCATCTTCTTCGCTGGGATTCTCTCAATCTCTGAGCATCCACGGAGGCATTTTCAGAAAGTGCATGGGGAGGGGTGCATTTCTATTTTGTATAAAGAACAAAGAAAACCCCTACTTATAATTGCGCCTGTAGCCATCAGGGGCTGTTTTGCTTGAAAGATTACAGGATTATGCAGCAGAACATACGACGGCATCTTTTACTTGCAACCAGATACTACTCTAGAATTTCTCCTCCACCACCTGCCTATGCCAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAAGGCCCAAAGGCCCGGCAGCTCCTATCCCTGCCCCCATTTCCTGGTTACCCTTTGCCAGGAAAGAATCAGATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCGTAAGATGGGTCAAGCATTACTTTGATGAAATTCAAGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGCCCACAATTGGGTGGGTTGGATGAAAAAGGAAATTTGAAACCTATGAGAAAGATTAGGAGTAGTGAGATTATGGAAACTGGGGCACGCATCCATATACCTGTGTCAGTTGCAGAGATGAAGGTTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAGAGACTTGTCAAGTACAAGGCAAGTATTGAGTTTAACTTGCATTCTCCTCTTTGCTGTTTGCATCAATTAATGAATCTCCCAGCGTTTTATCCATTGATATACAAGCAGTTGCTATATACTTGGTATGGAAAGATCCACATAGGTGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCAGTCAAGGGGAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCAGCTGCAGCATTATCTTATGACTACGATGATGGACCCAAGTTGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAGGAAAGTGTAGCCCACCTGCAATGGCTATTTGGCAATATAAGAAATGCGAAATCTACATGTAAGGCTTTGGACGAGGCATGTAATGCAACATATCAAAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATCTCTGCTCCTCTGTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCAAACAAAACATCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATAAATGGTTGTTCATGGATTGAACTATGTCCACGTACTAGCCGAAAACATCAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACACCCATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGAACGTGGAAACAAATGCCCCGAGTCGATGTTGAGCCGATAAGTGGGAGGCCATACAAGTTGCGAAGGCCAGAAGGGTTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCCTTATTACATCTTCACTGCAGAGAGCTCGTTCTTCCCAATTTTGCTAAGTTCCTTAATGTTTTGGATGATAAAAGATCGATCAGCCACCAGCCGGTATCTAGCTTGAACTCTGATCTCTTGCGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGAGTATCTTGAGTTTTCATAGGTGGATTTCATGCAACTAAAGGGAAATAAACATGATTGTAATAGGATTATCTATTTATTAAATTATTATTAGTCTTAGCCTTTTAGGTTAAACTATATGTTTACTCACTTAACGTTAAAAGTGTCGAATAAGTCTCTAAACTTTCAATTTTGTATCAAATCGATCATTGAATTTTAAGAAACGTGTAATAGGTTCTTGACCTTTCATTATTGTGTC

Coding sequence (CDS)

ATGCAGCAGAACATACGACGGCATCTTTTACTTGCAACCAGATACTACTCTAGAATTTCTCCTCCACCACCTGCCTATGCCAAGCCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGTAGTCCTAAAGAAGGCCCAAAGGCCCGGCAGCTCCTATCCCTGCCCCCATTTCCTGGTTACCCTTTGCCAGGAAAGAATCAGATTGGTGTCTCTGGGGAGTCAACTCATGTCACTGCCGTAAGATGGGTCAAGCATTACTTTGATGAAATTCAAGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGCCCACAATTGGGTGGGTTGGATGAAAAAGGAAATTTGAAACCTATGAGAAAGATTAGGAGTAGTGAGATTATGGAAACTGGGGCACGCATCCATATACCTGTGTCAGTTGCAGAGATGAAGGTTTCTAGGAGATTTGACACTATACCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAGAGACTTGTCAAGTACAAGGCAAGTATTGAGTTTAACTTGCATTCTCCTCTTTGCTGTTTGCATCAATTAATGAATCTCCCAGCGTTTTATCCATTGATATACAAGCAGTTGCTATATACTTGGTATGGAAAGATCCACATAGGTGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCAGTCAAGGGGAATCTTCCAGTTCATAATAGTATGGATGCGTTGGCAGCTGCAGCATTATCTTATGACTACGATGATGGACCCAAGTTGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAGGAAAGTGTAGCCCACCTGCAATGGCTATTTGGCAATATAAGAAATGCGAAATCTACATGTAAGGCTTTGGACGAGGCATGTAATGCAACATATCAAAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATCTCTGCTCCTCTGTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCAAACAAAACATCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATAAATGGTTGTTCATGGATTGAACTATGTCCACGTACTAGCCGAAAACATCAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACACCCATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGAACGTGGAAACAAATGCCCCGAGTCGATGTTGAGCCGATAAGTGGGAGGCCATACAAGTTGCGAAGGCCAGAAGGGTTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCCTTATTACATCTTCACTGCAGAGAGCTCGTTCTTCCCAATTTTGCTAAGTTCCTTAATGTTTTGGATGATAAAAGATCGATCAGCCACCAGCCGGTATCTAGCTTGAACTCTGATCTCTTGCGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTATGA

Protein sequence

MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGNLKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKASIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV

Homology

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BLAST of Lsi08G009290.1 vs. Swiss-Prot
Match: PUS3_ARATH (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.3e-130
Identity = 219/298 (73.49%), Postives = 257/298 (86.24%), Query Frame = 1


Query: 218 IHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILL 277
           +   DSAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+
Sbjct: 181 VRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLV 240

Query: 278 MGRTKESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSK 337
           MGRTKES+ +L  +F + +   S+CKA ++AC A YQ+YWALVIG P+E+EGLISAPLSK
Sbjct: 241 MGRTKESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSK 300

Query: 338 VLLDDGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAE 397
           VLLDDGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAE
Sbjct: 301 VLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAE 360

Query: 398 ALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHL 457
           ALGTPIVGDYKYGWFVH+ WKQMP+VD+EP +G+PYKLRRPEGLDVQKGSVLSKVPLLHL
Sbjct: 361 ALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHL 420

Query: 458 HCRELVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
           HCRE+VLPN AKFL+V++ + +           DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 421 HCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

[Close]

BLAST of Lsi08G009290.1 vs. Swiss-Prot
Match: PUS3_ORYSJ (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.1e-122
Identity = 205/294 (69.73%), Postives = 244/294 (82.99%), Query Frame = 1


Query: 222 DSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRT 281
           DSAI+VLNKPPK+P+KGNLPVHNSMD LAAAALSY  ++GPKLVHRLDRESSG+LL GRT
Sbjct: 183 DSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFGRT 242

Query: 282 KESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 341
           KES   L WLF ++  AK+  +  + AC A  Q+YWALVIG P+EREG+ISAPLSKVLLD
Sbjct: 243 KESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVLLD 302

Query: 342 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 401
           DGK ERV+LA+ + ++ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEALGT
Sbjct: 303 DGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEALGT 362

Query: 402 PIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 461
           PIVGDYKYGWFVH+ WKQ P+ D EP +G PYKLRRPEGL++QKGSVLSKVPLLHLHCRE
Sbjct: 363 PIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLHCRE 422

Query: 462 LVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
           +V+PN AKFL+   +    +  P S    +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 423 MVIPNIAKFLSSNGEWHE-NGAPWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475

[Close]

BLAST of Lsi08G009290.1 vs. Swiss-Prot
Match: PUS4_ARATH (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.2e-64
Identity = 129/291 (44.33%), Postives = 186/291 (63.92%), Query Frame = 1


Query: 222 DSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRT 281
           D AIIVLNKP  L V+G   +  S+D LAA+ L +D  + P+LVHRLDR+ SG+L++ RT
Sbjct: 189 DPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLART 248

Query: 282 KESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 341
           + +   L  +F   +   ++   + +   +  +KY ALVIGCP  + G ISAPL KV++D
Sbjct: 249 QTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVD 308

Query: 342 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 401
           DGK+ER+ + +   L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGT
Sbjct: 309 DGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGT 368

Query: 402 PIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 461
           PIVGDYKYGW  H+  +     +  P      +   P GLD+  G V SK P LHLH ++
Sbjct: 369 PIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDGGDVSSKQPHLHLHSKQ 428

Query: 462 LVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSS 513
           + LPN ++   +L+  +  S   +S L+S  L+F A +PSHM++S+NL+ S
Sbjct: 429 IDLPNISQ---LLEKMQVSSDSDISDLDS--LKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of Lsi08G009290.1 vs. Swiss-Prot
Match: PUS4_ORYSJ (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-35
Identity = 77/169 (45.56%), Postives = 111/169 (65.68%), Query Frame = 1


Query: 222 DSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRT 281
           D AIIV+NKPP +PV+G + + NS+D LA+     +  + P+LVHRLDR+ SGIL++GR 
Sbjct: 182 DEAIIVVNKPPGMPVQGGVGIKNSIDVLASM-FEENSSEAPRLVHRLDRDCSGILVLGRN 241

Query: 282 KESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 341
           + S + L  +F      K+     D   +   +KY ALVIG PR  +GL+SAPL+K+LL 
Sbjct: 242 QLSTSMLHAIF----REKTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQ 301

Query: 342 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQ 391
           DGK+ER+ +   ++  + Q+A+TEYRV+     G +W+EL PRT RKHQ
Sbjct: 302 DGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345

[Close]

BLAST of Lsi08G009290.1 vs. Swiss-Prot
Match: RUSD4_XENTR (RNA pseudouridylate synthase domain-containing protein 4 OS=Xenopus tropicalis GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 6.8e-26
Identity = 86/266 (32.33%), Postives = 127/266 (47.74%), Query Frame = 1


Query: 222 DSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYD---DGPKLVHRLDRESSGILLM 281
           D  ++++NKP  LPV G   V  S+ +L  A   + +    +  KL HRLDR+++G L++
Sbjct: 55  DPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDRDTTGALIL 114

Query: 282 GRTKESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKV 341
            RT E+   +Q             +AL E     ++ YWAL +G P  REG++  P+ + 
Sbjct: 115 ARTTEAAERVQ-------------QALRE--REVHRVYWALCLGTPSPREGILDIPIMEK 174

Query: 342 ------------------LLDDGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIE 401
                             + ++G  ERV +       ++ EA+T YR LG   +G S +E
Sbjct: 175 ETSGPQKHYKMALSPRFRVSEEGAVERVRVPR-----SAHEAVTRYRTLGAA-SGASLVE 234

Query: 402 LCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEG 461
           L P T  KHQLR+H A  L  PI+GD+KY  +  R   Q P          P  + R  G
Sbjct: 235 LHPITGVKHQLRVHLALGLNCPILGDHKYSHW-GRLAPQKP----------PDSVLRALG 284

Query: 462 LDVQKGSVLSKVPLLHLHCRELVLPN 467
           L V +   LS    LHLH  +L LP+
Sbjct: 295 LTVPQARTLS----LHLHAVQLTLPS 284

[Close]

BLAST of Lsi08G009290.1 vs. TrEMBL
Match: A0A0A0LQ04_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G402020 PE=4 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 2.7e-223
Identity = 401/515 (77.86%), Postives = 422/515 (81.94%), Query Frame = 1


Query: 1   MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQ+++RRHLLLATR YSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 73  MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 132

Query: 61  YPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQ+GVSGESTHVTA+RWVK+YFDEIQDS IQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 133 YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 192

Query: 121 LKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIR                              SG +      I     + + K 
Sbjct: 193 LKPMRKIR------------------------------SGEIMETGARIHLPVSVAEMKV 252

Query: 181 SIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNL 240
           +  F+   P   L+     P    + Y Q L      +   DSAIIVLNKPPKLPVKG L
Sbjct: 253 ARRFDT-IPSGTLY-----PNADEIKYLQRL------VKYKDSAIIVLNKPPKLPVKGTL 312

Query: 241 PVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKS 300
           PVHNSMDALA AALSYDYDDGPKLVHRLDRESSGILLMGRTKESV HLQWLF NIRNAK 
Sbjct: 313 PVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKF 372

Query: 301 TCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQ 360
           TCKA +EACNATYQKYWALVIGCPREREG+ISAPLSKVLLDDGKTERVVLANK S+EASQ
Sbjct: 373 TCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERVVLANKASIEASQ 432

Query: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQM 420
           EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR WKQM
Sbjct: 433 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQM 492

Query: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSI 480
           PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFLNV   K   
Sbjct: 493 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKG-- 543

Query: 481 SHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
            ++PVSS +SDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 553 PNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 543

[Close]

BLAST of Lsi08G009290.1 vs. TrEMBL
Match: D7SS16_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0054g00780 PE=4 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 8.8e-190
Identity = 340/513 (66.28%), Postives = 394/513 (76.80%), Query Frame = 1


Query: 9   LLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQ 68
           L    R+YSRI+PP PA A PVIRVSNN+AQLGSPKEGPK RQLLSLPPFP +PLPGK+ 
Sbjct: 12  LFPVVRHYSRIAPPRPASAAPVIRVSNNVAQLGSPKEGPKPRQLLSLPPFPAHPLPGKSL 71

Query: 69  IGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQL--GGLDEKGNLKPMRK 128
           + VSG  THVTA+ W+K+YFDEI  SV+QSHF KGLV +E   L    L+++G +KP+RK
Sbjct: 72  VNVSGLPTHVTAISWIKYYFDEIPGSVVQSHFNKGLVHMERSNLDDSSLEKEGQIKPLRK 131

Query: 129 IRSSEIMETGARIHIPVSVAEMKVSR---RFDTIPSGTLYPNADEIKYLQRLVKYKASIE 188
           I+ +E+ME G RI++PVSVAE   +R   RFDT                           
Sbjct: 132 IKPNEVMEAGTRIYVPVSVAE---ARISKRFDT--------------------------- 191

Query: 189 FNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNLPVH 248
                P   LH     P    + Y Q L+     +   D AI+VLNKPPK+P+KGNLPVH
Sbjct: 192 ----IPSGTLH-----PNADEIEYLQRLWL----LKFQDPAILVLNKPPKVPLKGNLPVH 251

Query: 249 NSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCK 308
           NSMDALAAAAL++DY++GPKLVHRLDRESSG+LLMGRTKESVAHL WLF +I  AKS  K
Sbjct: 252 NSMDALAAAALTFDYEEGPKLVHRLDRESSGLLLMGRTKESVAHLHWLFSDINKAKSLSK 311

Query: 309 ALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAI 368
           A D+AC AT Q+YWALVIG P+++EG+I APLSKVLLDDGKTERV+LAN++ LEASQEAI
Sbjct: 312 AWDDACEATCQRYWALVIGSPKQKEGVIHAPLSKVLLDDGKTERVILANQSGLEASQEAI 371

Query: 369 TEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRV 428
           TEYRVLGP INGCSWIELCPRTSRKHQLR+HCAEALGTPIVGDYKYGWFVHR WKQMPRV
Sbjct: 372 TEYRVLGPMINGCSWIELCPRTSRKHQLRVHCAEALGTPIVGDYKYGWFVHRRWKQMPRV 431

Query: 429 DVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSISHQ 488
           D+EP +G+PYK+RRPEGLDVQKGSVLSKVPLLHLHCREL+LPN AKFL +LD K    +Q
Sbjct: 432 DLEPTTGKPYKMRRPEGLDVQKGSVLSKVPLLHLHCRELILPNIAKFLPILDPKPKNQNQ 481

Query: 489 -PVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
            P  SL  D+LRFVA MP+HM+ISWNLMSSYLV
Sbjct: 492 YPKLSLKPDILRFVASMPNHMKISWNLMSSYLV 481

[Close]

BLAST of Lsi08G009290.1 vs. TrEMBL
Match: M5WPG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005240mg PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.2e-175
Identity = 324/505 (64.16%), Postives = 371/505 (73.47%), Query Frame = 1


Query: 11  LATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQIG 70
           +A R YSR+ PPP   A P+IRVSN++A+LG PKEGPK RQLLSLPPFPG+PLPGK    
Sbjct: 13  VARREYSRVCPPPAKSAGPLIRVSNHVARLGPPKEGPKPRQLLSLPPFPGHPLPGKRS-H 72

Query: 71  VSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGNLKPMRKIRSS 130
             G  + VTAV WVK+YFD I DS IQSHF++GLVQ+ECP  G ++++G +KPMRKIR S
Sbjct: 73  EGGVVSRVTAVSWVKYYFDGIYDSTIQSHFKQGLVQMECP--GMIEKEGQMKPMRKIRPS 132

Query: 131 EIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKASIEFNLHSPL 190
           E+ME GAR+++PVSV E +VSRRFD IPSGTLYPNADEI+Y+QR VKYK S    L+ P 
Sbjct: 133 EVMEVGARVYVPVSVVESRVSRRFDCIPSGTLYPNADEIEYMQRRVKYKDSAIIVLNKP- 192

Query: 191 CCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALA 250
             L    NLP    +         YG            NK PK                 
Sbjct: 193 PKLPVKGNLPVHNSMDALAAAALSYGN-----------NKGPK----------------- 252

Query: 251 AAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCKALDEACN 310
                        LVHRLDRESSG+ LMGRTKESV+ LQWL+ +    KS+C+A D+AC 
Sbjct: 253 -------------LVHRLDRESSGLHLMGRTKESVSLLQWLYTDFNKGKSSCQAWDDACE 312

Query: 311 ATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAITEYRVLG 370
           A YQ+YWALVIG P+E+EGLI APLSKVLLD+GKTERV+LA+++ LEASQ+AIT+YRVLG
Sbjct: 313 ARYQRYWALVIGSPKEKEGLICAPLSKVLLDNGKTERVILAHQSGLEASQKAITQYRVLG 372

Query: 371 PTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISG 430
           PTINGCSWIEL P TSRKHQLR+HCAEALGTPIVGDYKYGWFVHR WKQMP VDVEP SG
Sbjct: 373 PTINGCSWIELRPLTSRKHQLRVHCAEALGTPIVGDYKYGWFVHRRWKQMPHVDVEPTSG 432

Query: 431 RPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSISHQPVSSLNS 490
            PYKLRRP GLDVQKGSVLSKVPLLHLHCRELVLPN AKFL +LD K    H  VSS   
Sbjct: 433 LPYKLRRPAGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLLILDQKLDSLHPAVSS-KP 471

Query: 491 DLLRFVARMPSHMRISWNLMSSYLV 516
           D+LRFVA MPSHM+ISWNLMSSYLV
Sbjct: 493 DILRFVASMPSHMKISWNLMSSYLV 471

[Close]

BLAST of Lsi08G009290.1 vs. TrEMBL
Match: A0A022R4H6_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006040mg PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.2e-170
Identity = 310/502 (61.75%), Postives = 362/502 (72.11%), Query Frame = 1


Query: 17  SRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQIGVSGEST 76
           S+I PPPPA AK V+RVSNNIA LG+PKEGPK RQLLSLPPFPG PLPG+N   V     
Sbjct: 5   SKIRPPPPASAKAVLRVSNNIAHLGAPKEGPKPRQLLSLPPFPGNPLPGRNSTSVE---- 64

Query: 77  HVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDE--KGNLKPMRKIRSSEIME 136
           HVTA+ W+K+YFD++   VIQ HF KGLV++EC    GL E  +G     RKI+ SEI+E
Sbjct: 65  HVTAISWLKYYFDDVIPPVIQHHFNKGLVRMECLNSSGLSESYRGQTMSTRKIKPSEIVE 124

Query: 137 TGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKASIEFNLHSPLCCLH 196
            G R+++PVSVAE KVS+RFD                                 P   LH
Sbjct: 125 EGDRVYVPVSVAENKVSKRFDV-------------------------------IPSGTLH 184

Query: 197 QLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAAL 256
                P    ++Y Q L      +   DSAI+VLNKPPKLPVKGNLPVHNSMDALAAAAL
Sbjct: 185 -----PNADEIMYLQRL------VKYKDSAILVLNKPPKLPVKGNLPVHNSMDALAAAAL 244

Query: 257 SYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCKALDEACNATYQ 316
           SYDYD+GPKLVHRLDRESSG+LLMGRTKES+++L WLF ++R A S  K   +AC+A YQ
Sbjct: 245 SYDYDEGPKLVHRLDRESSGLLLMGRTKESISYLHWLFSDVRKAASFSKEWSDACDAMYQ 304

Query: 317 KYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAITEYRVLGPTIN 376
           +YWALVIG P ++EG+I APL+KVLL+DGKTE V+L++ + LEASQEAIT YRVLGP IN
Sbjct: 305 RYWALVIGVPNQKEGVICAPLTKVLLNDGKTEMVILSHNSGLEASQEAITRYRVLGPMIN 364

Query: 377 GCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYK 436
           GCSWIEL P T RKHQLR+HCAEALGTPIVGDYKYGWFVH  WKQMPR+D EP +G PYK
Sbjct: 365 GCSWIELHPLTRRKHQLRVHCAEALGTPIVGDYKYGWFVHSKWKQMPRLDFEPTTGEPYK 424

Query: 437 LRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDD-KRSISHQPVSSLNSDLL 496
           +RRPEGLDVQKGSVLSKVPLLHLH RE+VLPN  KF+ +      + +  P      D+L
Sbjct: 425 MRRPEGLDVQKGSVLSKVPLLHLHSREMVLPNIEKFIELHKHMSGNYNVDPELCAKPDIL 460

Query: 497 RFVARMPSHMRISWNLMSSYLV 516
           RFV  MP HM+ISWNLMSSYLV
Sbjct: 485 RFVVPMPKHMKISWNLMSSYLV 460

[Close]

BLAST of Lsi08G009290.1 vs. TrEMBL
Match: W9SMU4_9ROSA (RNA pseudourine synthase 3 OS=Morus notabilis GN=L484_000552 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.0e-169
Identity = 317/505 (62.77%), Postives = 369/505 (73.07%), Query Frame = 1


Query: 14  RYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQIGVSG 73
           R YSRI PP  A A PVIRVSNN+AQLG PKEGPK RQLLSLPPFPG PLPGK       
Sbjct: 7   RQYSRIRPPAAAEAGPVIRVSNNVAQLGGPKEGPKPRQLLSLPPFPGLPLPGK------- 66

Query: 74  ESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGG--LDEKGNLKPMRKIRSSE 133
           +S  VTA+ WVK+YF+E+ D+VIQSHF K LVQ+EC   G    +    +KPMRKI+ +E
Sbjct: 67  KSERVTAITWVKYYFEEVHDNVIQSHFNKSLVQMECSNFGDPLAERDRKMKPMRKIKPNE 126

Query: 134 IMETGAR-IHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKASIEFNLHSPL 193
           +M  G   I++PVSV                               + + S  F++  P 
Sbjct: 127 VMVEG-TRIYVPVSV------------------------------AESRISKRFDV-IPS 186

Query: 194 CCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALA 253
             LH     P    + Y Q L          DSAI+VLNKPP+LPVKG+LPVHNSMDALA
Sbjct: 187 GTLH-----PNADEIAYLQRL----------DSAILVLNKPPRLPVKGHLPVHNSMDALA 246

Query: 254 AAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCKALDEACN 313
           AAALSYDY +GPKLVHRLDR+SSG+LLMGRTKES++HL+WLF NI       KA ++AC 
Sbjct: 247 AAALSYDYSEGPKLVHRLDRDSSGLLLMGRTKESISHLEWLFSNI-------KAWNDACE 306

Query: 314 ATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAITEYRVLG 373
           A YQKYWALVIG P+E+EG+I APLSKVLL+DGKTERV+LA++T  +ASQEAITEYRVLG
Sbjct: 307 AKYQKYWALVIGSPKEKEGIICAPLSKVLLNDGKTERVILAHQTGFQASQEAITEYRVLG 366

Query: 374 PTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISG 433
           P INGCSW+EL P TSRKHQLR+HCAEALGTPIVGDYKYGWFVH+ WKQMP+V++EP +G
Sbjct: 367 PMINGCSWLELRPLTSRKHQLRVHCAEALGTPIVGDYKYGWFVHKRWKQMPQVNIEPTTG 426

Query: 434 RPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSISHQPVSSLNS 493
           +PYKLRRPEGLDVQKGSV+SKVPLLHL CRELVLPN AKFL V  D++S +  P      
Sbjct: 427 KPYKLRRPEGLDVQKGSVMSKVPLLHLQCRELVLPNIAKFLRVF-DRKSQNQDPALESKP 449

Query: 494 DLLRFVARMPSHMRISWNLMSSYLV 516
           DLLRFVARMPSHM+ISWNLMSSYLV
Sbjct: 487 DLLRFVARMPSHMKISWNLMSSYLV 449

[Close]

BLAST of Lsi08G009290.1 vs. TAIR10
Match: AT1G78910.1 (AT1G78910.1 Pseudouridine synthase family protein)

HSP 1 Score: 468.0 bits (1203), Expect = 7.5e-132
Identity = 219/298 (73.49%), Postives = 257/298 (86.24%), Query Frame = 1


Query: 218 IHIGDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILL 277
           +   DSAIIVLNKPPKLPVKGN+P+HNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+
Sbjct: 181 VRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLV 240

Query: 278 MGRTKESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSK 337
           MGRTKES+ +L  +F + +   S+CKA ++AC A YQ+YWALVIG P+E+EGLISAPLSK
Sbjct: 241 MGRTKESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSK 300

Query: 338 VLLDDGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAE 397
           VLLDDGKT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAE
Sbjct: 301 VLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAE 360

Query: 398 ALGTPIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHL 457
           ALGTPIVGDYKYGWFVH+ WKQMP+VD+EP +G+PYKLRRPEGLDVQKGSVLSKVPLLHL
Sbjct: 361 ALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHL 420

Query: 458 HCRELVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
           HCRE+VLPN AKFL+V++ + +           DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 421 HCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

[Close]

BLAST of Lsi08G009290.1 vs. TAIR10
Match: AT3G19440.1 (AT3G19440.1 Pseudouridine synthase family protein)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-65
Identity = 129/291 (44.33%), Postives = 186/291 (63.92%), Query Frame = 1


Query: 222 DSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRT 281
           D AIIVLNKP  L V+G   +  S+D LAA+ L +D  + P+LVHRLDR+ SG+L++ RT
Sbjct: 189 DPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVLART 248

Query: 282 KESVAHLQWLFGNIRNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLD 341
           + +   L  +F   +   ++   + +   +  +KY ALVIGCP  + G ISAPL KV++D
Sbjct: 249 QTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLRKVVVD 308

Query: 342 DGKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGT 401
           DGK+ER+ + +   L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCAE LGT
Sbjct: 309 DGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGT 368

Query: 402 PIVGDYKYGWFVHRTWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE 461
           PIVGDYKYGW  H+  +     +  P      +   P GLD+  G V SK P LHLH ++
Sbjct: 369 PIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDGGDVSSKQPHLHLHSKQ 428

Query: 462 LVLPNFAKFLNVLDDKRSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSS 513
           + LPN ++   +L+  +  S   +S L+S  L+F A +PSHM++S+NL+ S
Sbjct: 429 IDLPNISQ---LLEKMQVSSDSDISDLDS--LKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of Lsi08G009290.1 vs. TAIR10
Match: AT1G76050.2 (AT1G76050.2 Pseudouridine synthase family protein)

HSP 1 Score: 60.1 bits (144), Expect = 4.7e-09
Identity = 48/197 (24.37%), Postives = 84/197 (42.64%), Query Frame = 1


Query: 247 DALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKSTCKALD 306
           + +  +  SY     P +VHRLD+ ++G+L++ + + S AHL   F              
Sbjct: 212 EEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQF-------------- 271

Query: 307 EACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQEAITEY 366
              +   + Y +L  G P   +G I  P+ +      +     +        ++ A + Y
Sbjct: 272 -KLHTIERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAASRY 331

Query: 367 RVLGPTINGCS-WIELCPRTSRKHQLRIHCAEALGTPIVGDYKYG----WFVHRTWKQMP 426
           +V+     G S  +E    T R HQ+R H A+ +G P++GD  YG      +    K++ 
Sbjct: 332 KVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYGGTKSMALSLLQKRVS 390

Query: 427 RVDVEPISGRPYKLRRP 439
           R D E I     ++ RP
Sbjct: 392 RSDQEEIIELISRMDRP 390

[Close]

BLAST of Lsi08G009290.1 vs. NCBI nr
Match: gi|778672934|ref|XP_011649894.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 782.7 bits (2020), Expect = 3.9e-223
Identity = 401/515 (77.86%), Postives = 422/515 (81.94%), Query Frame = 1


Query: 1   MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQ+++RRHLLLATR YSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQ+GVSGESTHVTA+RWVK+YFDEIQDS IQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120

Query: 121 LKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIR                              SG +      I     + + K 
Sbjct: 121 LKPMRKIR------------------------------SGEIMETGARIHLPVSVAEMKV 180

Query: 181 SIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNL 240
           +  F+   P   L+     P    + Y Q L      +   DSAIIVLNKPPKLPVKG L
Sbjct: 181 ARRFDT-IPSGTLY-----PNADEIKYLQRL------VKYKDSAIIVLNKPPKLPVKGTL 240

Query: 241 PVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKS 300
           PVHNSMDALA AALSYDYDDGPKLVHRLDRESSGILLMGRTKESV HLQWLF NIRNAK 
Sbjct: 241 PVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKF 300

Query: 301 TCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQ 360
           TCKA +EACNATYQKYWALVIGCPREREG+ISAPLSKVLLDDGKTERVVLANK S+EASQ
Sbjct: 301 TCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERVVLANKASIEASQ 360

Query: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQM 420
           EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR WKQM
Sbjct: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQM 420

Query: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSI 480
           PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFLNV   K   
Sbjct: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKG-- 471

Query: 481 SHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
            ++PVSS +SDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 481 PNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 471

[Close]

BLAST of Lsi08G009290.1 vs. NCBI nr
Match: gi|700207975|gb|KGN63094.1| (hypothetical protein Csa_2G402020 [Cucumis sativus])

HSP 1 Score: 782.7 bits (2020), Expect = 3.9e-223
Identity = 401/515 (77.86%), Postives = 422/515 (81.94%), Query Frame = 1


Query: 1   MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQ+++RRHLLLATR YSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 73  MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 132

Query: 61  YPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQ+GVSGESTHVTA+RWVK+YFDEIQDS IQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 133 YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 192

Query: 121 LKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIR                              SG +      I     + + K 
Sbjct: 193 LKPMRKIR------------------------------SGEIMETGARIHLPVSVAEMKV 252

Query: 181 SIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNL 240
           +  F+   P   L+     P    + Y Q L      +   DSAIIVLNKPPKLPVKG L
Sbjct: 253 ARRFDT-IPSGTLY-----PNADEIKYLQRL------VKYKDSAIIVLNKPPKLPVKGTL 312

Query: 241 PVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKS 300
           PVHNSMDALA AALSYDYDDGPKLVHRLDRESSGILLMGRTKESV HLQWLF NIRNAK 
Sbjct: 313 PVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKF 372

Query: 301 TCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQ 360
           TCKA +EACNATYQKYWALVIGCPREREG+ISAPLSKVLLDDGKTERVVLANK S+EASQ
Sbjct: 373 TCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERVVLANKASIEASQ 432

Query: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQM 420
           EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR WKQM
Sbjct: 433 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQM 492

Query: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSI 480
           PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFLNV   K   
Sbjct: 493 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKG-- 543

Query: 481 SHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
            ++PVSS +SDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 553 PNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 543

[Close]

BLAST of Lsi08G009290.1 vs. NCBI nr
Match: gi|659081205|ref|XP_008441208.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial [Cucumis melo])

HSP 1 Score: 780.8 bits (2015), Expect = 1.5e-222
Identity = 402/515 (78.06%), Postives = 419/515 (81.36%), Query Frame = 1


Query: 1   MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQ++IRRHLLLATRYYSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSIRRHLLLATRYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGN 120
           +PLPGKNQ+GVSGESTHVTA+RWVK+YFDEIQDS IQS FRKGLVQVECPQLGG DE GN
Sbjct: 61  HPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSLFRKGLVQVECPQLGGFDENGN 120

Query: 121 LKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKI                               SG +      I     + + K 
Sbjct: 121 LKPMRKI------------------------------RSGEIMEIGARIHLPVSVAEMKV 180

Query: 181 SIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNL 240
           S  F+   P   L+     P    + Y Q L      +   DSAIIVLNKPPKLPVKG L
Sbjct: 181 SRRFDT-IPSGTLY-----PNADEIKYLQRL------VKYKDSAIIVLNKPPKLPVKGTL 240

Query: 241 PVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKS 300
           PVHNSMDALAAAALSYDYD GPKLVHRLDRESSGILLMGRTKESV HLQWLF NIRN K 
Sbjct: 241 PVHNSMDALAAAALSYDYDYGPKLVHRLDRESSGILLMGRTKESVDHLQWLFSNIRNGKF 300

Query: 301 TCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQ 360
           TCKA +EACNATYQKYWALVIG PREREGLISAPLSKVLLDDGKTERVVLANK SLEASQ
Sbjct: 301 TCKAWEEACNATYQKYWALVIGRPREREGLISAPLSKVLLDDGKTERVVLANKASLEASQ 360

Query: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQM 420
           EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR WKQM
Sbjct: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQM 420

Query: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSI 480
           PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFLNV  DK  I
Sbjct: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLNVFYDKGPI 473

Query: 481 SHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
           SH+PVSS + DLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 481 SHRPVSSFDFDLLRFVARMPSHMRISWNLMSSYLV 473

[Close]

BLAST of Lsi08G009290.1 vs. NCBI nr
Match: gi|778672937|ref|XP_011649895.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 716.5 bits (1848), Expect = 3.4e-203
Identity = 376/515 (73.01%), Postives = 397/515 (77.09%), Query Frame = 1


Query: 1   MQQNIRRHLLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQ+++RRHLLLATR YSRISPPPPAYAKP+IRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQ+GVSGESTHVTA+RWVK+YFDEIQDS IQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120

Query: 121 LKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKA 180
           LKPMRKIR                              SG +      I     + + K 
Sbjct: 121 LKPMRKIR------------------------------SGEIMETGARIHLPVSVAEMKV 180

Query: 181 SIEFNLHSPLCCLHQLMNLPAFYPLIYKQLLYTWYGKIHIGDSAIIVLNKPPKLPVKGNL 240
           +  F+   P   L+     P    + Y Q L      +   DSAIIVLNKPPKLPVK   
Sbjct: 181 ARRFDT-IPSGTLY-----PNADEIKYLQRL------VKYKDSAIIVLNKPPKLPVK--- 240

Query: 241 PVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNIRNAKS 300
                                   VHRLDRESSGILLMGRTKESV HLQWLF NIRNAK 
Sbjct: 241 ------------------------VHRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKF 300

Query: 301 TCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTSLEASQ 360
           TCKA +EACNATYQKYWALVIGCPREREG+ISAPLSKVLLDDGKTERVVLANK S+EASQ
Sbjct: 301 TCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERVVLANKASIEASQ 360

Query: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQM 420
           EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR WKQM
Sbjct: 361 EAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQM 420

Query: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLDDKRSI 480
           PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFLNV   K   
Sbjct: 421 PRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKG-- 444

Query: 481 SHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
            ++PVSS +SDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 481 PNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 444

[Close]

BLAST of Lsi08G009290.1 vs. NCBI nr
Match: gi|1012257060|ref|XP_015945118.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X3 [Arachis duranensis])

HSP 1 Score: 684.1 bits (1764), Expect = 1.9e-193
Identity = 348/525 (66.29%), Postives = 413/525 (78.67%), Query Frame = 1


Query: 9   LLLATRYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKN- 68
           L    R+YSRI PP P   + VIRVSNN+A+LGS KEGPK RQLLSLPPFPG+PLPG+N 
Sbjct: 13  LWFVARHYSRIRPPKPVPVESVIRVSNNVARLGSRKEGPKPRQLLSLPPFPGHPLPGRNS 72

Query: 69  ----QIGVSGESTHVTAVRWVKHYFDEIQDSVIQSHFRKGLVQVECP----QLGGLDEKG 128
               Q G   +  +VTAV W+K+YF  I  SVI+SHFR+GLVQ+E P     L   +E  
Sbjct: 73  NESFQDGQQHDG-YVTAVNWIKYYFKGILGSVIESHFREGLVQMEDPISDNNLSQNEEPR 132

Query: 129 NLKPMRKIRSSEIMETGARIHIPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYK 188
             +P+RKIR +E+M+ GARIHIPVS+AE ++S+R+D IPSGTLYPNADEIKYLQRLV YK
Sbjct: 133 --RPLRKIRPNEVMKQGARIHIPVSIAEARISKRYDAIPSGTLYPNADEIKYLQRLVIYK 192

Query: 189 ASIEFNLHSPLCCLHQLMNLPAFYPLI----YKQLLYTWYGKIHIGDSAIIVLNKPPKLP 248
           A +  N+   L   +  + L +F+  +       +L + Y   ++ DSAI+VLNKPPKLP
Sbjct: 193 ACLASNVK--LILFYNFLLLFSFFKCMAIYFIDCILCSMY---YLKDSAIMVLNKPPKLP 252

Query: 249 VKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFGNI 308
           VKGNLPVHNSMDALAAAALSYDYD+GPKLVHRLDRE+SGI+L GR ++SV+HLQWLF NI
Sbjct: 253 VKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRETSGIILFGRKEQSVSHLQWLFSNI 312

Query: 309 RNAKSTCKALDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANKTS 368
            NAKS+ K  ++AC +TYQ+YWALVIG P+E+EG+I APL KVLLDDGKTERVVLA+ +S
Sbjct: 313 NNAKSSSKVWNDACESTYQRYWALVIGTPKEKEGVICAPLRKVLLDDGKTERVVLAHHSS 372

Query: 369 LEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHR 428
           +E  QEA+TEYRVLGP INGCSW+EL P T RKHQLR+HCAEALGTPIVGDYKYGWFVH 
Sbjct: 373 MEPRQEAVTEYRVLGPKINGCSWVELRPLTYRKHQLRVHCAEALGTPIVGDYKYGWFVHS 432

Query: 429 TWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNFAKFLNVLD 488
            WKQMPRVDVEP +G+PYK+RRPEGLDVQKGSVLSKVPLLHLHCRELVLPN AKFL+V +
Sbjct: 433 RWKQMPRVDVEPTTGKPYKMRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFLHVFN 492

Query: 489 DK-----RSISHQPVSSLNSDLLRFVARMPSHMRISWNLMSSYLV 516
                  RS+S +P      D+LRFVA MPSHM+ISWNLMSSYLV
Sbjct: 493 KSSGELPRSLSAEP------DVLRFVATMPSHMKISWNLMSSYLV 523

The following BLAST results are available for this feature:

BLAST of Lsi08G009290.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PUS3_ARATH	1.3e-130	73.49	RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana GN=At1g78910...	[more]
PUS3_ORYSJ	5.1e-122	69.73	RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica GN=O...	[more]
PUS4_ARATH	2.2e-64	44.33	RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana GN=At3g19440...	[more]
PUS4_ORYSJ	2.8e-35	45.56	RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica GN=O...	[more]
RUSD4_XENTR	6.8e-26	32.33	RNA pseudouridylate synthase domain-containing protein 4 OS=Xenopus tropicalis G...	[more]

BLAST of Lsi08G009290.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQ04_CUCSA	2.7e-223	77.86	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G402020 PE=4 SV=1	[more]
D7SS16_VITVI	8.8e-190	66.28	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0054g00780 PE=4 SV=...	[more]
M5WPG3_PRUPE	1.2e-175	64.16	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005240mg PE=4 SV=1	[more]
A0A022R4H6_ERYGU	1.2e-170	61.75	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006040mg PE=4 SV=1	[more]
W9SMU4_9ROSA	1.0e-169	62.77	RNA pseudourine synthase 3 OS=Morus notabilis GN=L484_000552 PE=4 SV=1	[more]

BLAST of Lsi08G009290.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G78910.1	7.5e-132	73.49	Pseudouridine synthase family protein	[more]
AT3G19440.1	1.2e-65	44.33	Pseudouridine synthase family protein	[more]
AT1G76050.2	4.7e-09	24.37	Pseudouridine synthase family protein	[more]

BLAST of Lsi08G009290.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778672934\|ref\|XP_011649894.1\|	3.9e-223	77.86	PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X1 [Cucumis sativ...	[more]
gi\|700207975\|gb\|KGN63094.1\|	3.9e-223	77.86	hypothetical protein Csa_2G402020 [Cucumis sativus]	[more]
gi\|659081205\|ref\|XP_008441208.1\|	1.5e-222	78.06	PREDICTED: RNA pseudouridine synthase 3, mitochondrial [Cucumis melo]	[more]
gi\|778672937\|ref\|XP_011649895.1\|	3.4e-203	73.01	PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X2 [Cucumis sativ...	[more]
gi\|1012257060\|ref\|XP_015945118.1\|	1.9e-193	66.29	PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X3 [Arachis duran...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0009982	pseudouridine synthase activity
GO:0003723	RNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0009451	RNA modification
GO:0001522	pseudouridine synthesis

Vocabulary: **INTERPRO**
Term	Definition
IPR020103	PsdUridine_synth_cat_dom_sf
IPR006145	PsdUridine_synth_RsuA/RluD

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:0031119	tRNA pseudouridine synthesis
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0019239	deaminase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi08G009290	Lsi08G009290	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi08G009290.1	Lsi08G009290.1-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G009290.1.three_prime_UTR.1	Lsi08G009290.1.three_prime_UTR.1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G009290.1.CDS.9	Lsi08G009290.1.CDS.9	CDS
Lsi08G009290.1.CDS.8	Lsi08G009290.1.CDS.8	CDS
Lsi08G009290.1.CDS.7	Lsi08G009290.1.CDS.7	CDS
Lsi08G009290.1.CDS.6	Lsi08G009290.1.CDS.6	CDS
Lsi08G009290.1.CDS.5	Lsi08G009290.1.CDS.5	CDS
Lsi08G009290.1.CDS.4	Lsi08G009290.1.CDS.4	CDS
Lsi08G009290.1.CDS.3	Lsi08G009290.1.CDS.3	CDS
Lsi08G009290.1.CDS.2	Lsi08G009290.1.CDS.2	CDS
Lsi08G009290.1.CDS.1	Lsi08G009290.1.CDS.1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G009290.1.five_prime_UTR.3	Lsi08G009290.1.five_prime_UTR.3	five_prime_UTR
Lsi08G009290.1.five_prime_UTR.2	Lsi08G009290.1.five_prime_UTR.2	five_prime_UTR
Lsi08G009290.1.five_prime_UTR.1	Lsi08G009290.1.five_prime_UTR.1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	PFAM	PF00849	PseudoU_synth_2	coord: 225..396 score: 9.2
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 455..467 score: 6.58E-37coord: 217..427 score: 6.58
None	No IPR available	PANTHER	PTHR11079	CYTOSINE DEAMINASE	coord: 130..155 score: 3.3E-95coord: 217..466 score: 3.3E-95coord: 487..514 score: 3.3
None	No IPR available	PANTHER	PTHR11079:SF74	RNA PSEUDOURIDYLATE SYNTHASE DOMAIN-CONTAINING PROTEIN 4	coord: 130..155 score: 3.3E-95coord: 217..466 score: 3.3E-95coord: 487..514 score: 3.3