Lsi08G001690.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi08G001690.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Carboxylesterase-like protein
Location	chr08 : 3503484 .. 3506800 (+)
Sequence length	1301

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTTTTTGATATGATTGGAGGAGCTGCAATATATAGAGGCTTAAAAGCAAACAAAAGTGACCTAAACTAAGTATACTCTGATCCATTTCATATTTTCCATAACTAAGTATACTCTGATCCATTTCATATTTTCCATAATAAAAAAGCCATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTGATCAGTAATCGATGCTATTCAAATGCTTTTTTTTTTATTAATTATTTTAAATGGCTCAAAAGAAGAGATATCTCCAATCCTAATATATAAATAATACCAATAAAGATTACTAAAAATATTTATAAATAAATAAAAATAAAGTACTTCTTTTATTTCATCCGAAAAGTCTTTTTCTTTTTAGTTCCACGGCCGATCAGTACCTAGCCGGGCTTTAACTCTTAAAAATATTTTAAATATAGCAAAATAACGCGAGAATATGTATTGATGTATATCTATTAGTATGTATCCCTTATCTATGGATAGATGTTTATAAGTATCTTATAAGTATCTATCATTATTTATCACAAACATACACTGATATTTTGCTATATTTTTAAATAAGTTGGCTCATTTTTTTTATATATTTAAATTTTTTTTTTAATGTTTTCTTCCTCCAAATGCTGCTTATTGTTTTAGTTTTGTACTTTCGGTTAATTTGAATTATATTATATTAAATTTAGTCTGTCAAAGTTAGTTTCTATATAAATTAGTTAAATAATAATAATAATTTTTATGTAAAAAAAATTTAATAGTGTGAATATGTTTTTAACATTTATAGTATAAATGTTAATAGATAATAAAAAAAATTTTAAAAGATCAATACATAATTTAAAATATATTAAAAATATAAAGATTAAAATTGAATAAACTTTTGAAAAGATAGATATTAAAATAATATTTTAGCCTTAATTTAATGTATTATTGATTCCACCTGTACATAGTTATTAAAATAACATTTTTGTGTTTTTTAAATTTAAATTTTCGAATTTGAAAATGTTGAATTACATCAGCATTGGTTTTTTATGAATATATTATTTTATGTTTTGGTTATAATTTTTAATTTAACCTTTTGTCATTTTGTATAATGTATTAAAATATTTGGTTAATAACTTTTTGTTAAAAGATTCTTATTATTTTGAAACTCGTTCAATTTTAATTTTATTATTGATTTTTTTATTATCTATTAGAATTTCTATAATAATTTTAAAAATGTATTCACATGAAAAAAAATATTATTATTATTTAACCAATTTTGATAAAAATAAATTTAGAGAAATTAATAAATTTTAAAATTTATTGAAAATATCAAGATTAAAATTGTAATTTTAAAGTATTTACAATAAAATTTAATAAACTCTAAACTATGTGAGACTAAAATGATACCTTTAACCTTTTATTTATTTTTTAATAAATATAACATTAAATGTTCTTGTTCTTTGAATAAGCTTGTGTACTTAACCATCAATGACTCCATAATAATTCGAATCATCCATCTCCTATTCTACATACCTAAAAATAAAAAAAAGAAGTGACATCTAATTTCTAATTCCGCTATCAAATATATATTAAAAACACTATAAACAATAAATATATTATATAATACTATATAGTGGAAGTCTGTTTTTAATTAAATTTCTTATTCTTACATCTATTTTGATATTATTAATTAATCCTCTCAATCTTAAGTTTAAAGGATTATTTTTAAATATAGAAAAATGAGTTAAAATATTTATAAATATAGAAAAATTTCATTGTCTACCAACGAAAACCATTATCGCTCAGTGATAGAAGTTTATTGTGGTCTATCGCTGATAACAGTGATAATTTTCTATATTTGTAACTAGTTTCATATTTTTTTCATTTATAATAATTTTTCAAATTTTAAAAGCATTATTAAATTACTTATTGTAAATCAAACTTAACACTTCATTCATGTTTTTAACTTTTTTTTTTTTTAGTAAGCTTAAAAATTAGCACAAAGTCATCAATAATTCTATATTGATATCATGTTAACTTAATTCTATATCAATATAATGTTTAACATTAATTTTATATTAATATTCAGATCAATTTATAAAGTTTTTATTAATAGGCTTAAATCACGGACCTCTTAGTCATTAACTTAAATTTTTTTTATTATTAATTAAGGTAAATTCAAATTAGTAACATTTCATTCCTATTATTTAACAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAAATTGGAAGATCTATATGGCTTTGCCTTGGATGTTTATTAGTGTGTGTTTTTAATTTGGCTTTCTAAGTGATAAGTTGCAATTTCATTCTTTGAATATTGATAAATTCTAAAAACATCTGTTCTCTAAAATTTGAATAAGAGTTTTGTTACCATCTA

mRNA sequence

ACTTTTTTGATATGATTGGAGGAGCTGCAATATATAGAGGCTTAAAAGCAAACAAAAGTGACCTAAACTAAGTATACTCTGATCCATTTCATATTTTCCATAACTAAGTATACTCTGATCCATTTCATATTTTCCATAATAAAAAAGCCATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAAATTGGAAGATCTATATGGCTTTGCCTTGGATGTTTATTAGTGTGTGTTTTTAATTTGGCTTTCTAAGTGATAAGTTGCAATTTCATTCTTTGAATATTGATAAATTCTAAAAACATCTGTTCTCTAAAATTTGAATAAGAGTTTTGTTACCATCTA

Coding sequence (CDS)

ATGGAAAAGGTTCCATTAATATTCAGAGCGTTTCTGAGAGTCCAATCCGCCGCCTTGAACGTCAGTCTCCGGCCGAATAGGACTGTGAACCGTTTCATAATGAATCTCTTCGACCCAAAAATCCCTCCTTCTTCTAAACCAAACGACGGCGTTACAACTCACGACGTCGTTTTTGACTCAACTCGTAATCTATGGTTTCGCGTCTTTGTGCCTTGTTCAGACGATTCTGATAATCTTACCGGCCTTCCTCTGCTTGTGTACTTTCACGGTGGAGGCTTTTCGTTTCTTGCAGCTAATTCTATGCCTTTCGTTGACCATTGTCGGAGACTCGCACGTGACCTCCGTGTTGTGGTTGTTTCCGTCAACTATCGTCTCGCTCCTGAACATCAATACCCAATTCCCTATGAAGATGGATTTGATGCTTTGAAATTTCTTGACGATTTGGGATCTGATAATTCTGATGTTTTTCCGGTTAAAGTTGATTTTGATCGGTGTTTTCTCGCCGGTGATAGTGCCGGCGGAAATTTGGCCCACCACGTTGCAGTCAGAGCGGGCGGTTATCATTTCAAGAAGCTTAACATTAAAGGTTTAATTGCAATACAACCATTTTTCGGCGGGGAAGAGCGAGTAGAGTCGGAAGTTCAATACGGTAAGTCGGCAATGTTAAACTTGGAACAAGCGGATTGGTATTGGAAGGCATTTCTACCACATGGGTCCGACAGAAACCACCCAGCAGCACACGTGTACGGTCCCGACGCTGAAGACATCTCCGGGGTGAAGTTTCCGGCGACACTTCTGATCGTGGGAGGACAAGACCAGTTACAAGATTGGCAAAAAAGGTATTATGAGTGGCTGAAAGAGGCTGGAAAAGAAGTTGATTTGGTTGAATTTCCAGATGCTGTCCATGGATTTTATTTAGTTCCTGGGCTGGAGGATGATACTTCATTGTTCATCAAACATGCAAGTGAATTCATACTCAAACATATGGCAGTGTAA

Protein sequence

MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFILKHMAV

Homology

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BLAST of Lsi08G001690.1 vs. Swiss-Prot
Match: CXE18_ARATH (Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.6e-81
Identity = 148/320 (46.25%), Postives = 213/320 (66.56%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           +PL  R  L V S   + + RP+ T+NR  + LFD + PP+ KP + V+T D V D +R+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRVFVP-CSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 123
           LWFR++ P  S D      +P++V+FHGGGF+FL+ N+ P+ + CRR AR L   V+SVN
Sbjct: 73  LWFRLYTPHVSGDK-----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 124 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 183
           YRLAPEH+YP  Y+DGFDALK++++   ++  + P   D  RCF AGDSAGGN+AH+VA+
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEE---NHGSILPANADLSRCFFAGDSAGGNIAHNVAI 192

Query: 184 ---RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 243
              R     F  + + GLI+IQPFFGGEER E+E Q   + +++ ++ DW WKA    G 
Sbjct: 193 RICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GL 252

Query: 244 DRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPD 303
           +R+H A +V GP+A DISG+ +P T+++V G D L+DWQ+ YYEWLK  GK+  L+E+P+
Sbjct: 253 NRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPN 312

Query: 304 AVHGFYLVPGLEDDTSLFIK 320
             H FY+ P L +   L ++
Sbjct: 313 MFHAFYIFPELPEAGQLIMR 321

[Close]

BLAST of Lsi08G001690.1 vs. Swiss-Prot
Match: GID1C_ARATH (Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.8e-63
Identity = 130/315 (41.27%), Postives = 185/315 (58.73%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+P ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRVFVPC-SDDSDNLTGL---------PLLVYFHGGGFSFLAANSMPFVDHCRRLARD 123
           L  RV+ P  +  S ++T L         P++V+FHGG F+  +ANS  +   CRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAG 183
              VVVSVNYR APE++YP  Y+DG+  LK++    + +S +   K    R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV----NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 184 GNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKA 243
           GN+ H+VAVRA      ++++ G I + P FGG ER ESE +      + +   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 244 FLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVD 303
           FLP G DR HPA   +GP ++ + G+ FP +L++V G D +QDWQ +Y E LK+AG+EV 
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 304 LVEFPDAVHGFYLVP 309
           L+    A  GFYL+P
Sbjct: 316 LLYLEQATIGFYLLP 323

[Close]

BLAST of Lsi08G001690.1 vs. Swiss-Prot
Match: GID1B_ARATH (Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.0e-59
Identity = 130/339 (38.35%), Postives = 188/339 (55.46%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A  V  RP+ + NR +    D K+P +S P DGV + D V DST N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV-DSTTN 75

Query: 64  LWFRVFVPCSDDSDNLTG-------------LPLLVYFHGGGFSFLAANSMPFVDHCRRL 123
           L  R++ P S       G             +P+L++FHGG F+  +ANS  +   CRRL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 124 ARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDL----GSDNSDVFPVKVDFDRCF 183
                VVVVSV+YR +PEH+YP  Y+DG++AL ++          +S+V+         +
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY--------VY 195

Query: 184 LAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQ 243
           LAGDS+GGN+AH+VAVRA     K L   G I + P FGG+ER +SE        + ++ 
Sbjct: 196 LAGDSSGGNIAHNVAVRATNEGVKVL---GNILLHPMFGGQERTQSEKTLDGKYFVTIQD 255

Query: 244 ADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK 303
            DWYW+A+LP G DR+HPA + +GP  + + GV FP +L++V G D +QDWQ  Y + LK
Sbjct: 256 RDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLK 315

Query: 304 EAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFI 326
           + G EV+L+    A  GFY +P   D     ++  ++F+
Sbjct: 316 KTGLEVNLLYLKQATIGFYFLPN-NDHFHCLMEELNKFV 341

[Close]

BLAST of Lsi08G001690.1 vs. Swiss-Prot
Match: GID1A_ARATH (Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.0e-51
Identity = 105/243 (43.21%), Postives = 147/243 (60.49%), Query Frame = 1


Query: 82  LPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDA 141
           +P++++FHGG F+  +ANS  +   CRRL    + VVVSVNYR APE+ YP  Y+DG+ A
Sbjct: 106 VPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIA 165

Query: 142 LKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQ 201
           L +++      S +   K      FLAGDS+GGN+AH+VA+RAG      +++ G I + 
Sbjct: 166 LNWVNS----RSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLN 225

Query: 202 PFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKF 261
           P FGG ER ESE        + +   DWYWKAFLP G DR HPA + + P  + + GV F
Sbjct: 226 PMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSF 285

Query: 262 PATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVHGFYLVP------GLEDDTS 319
           P +L++V G D ++DWQ  Y E LK+AG+EV L+    A  GFYL+P       + D+ S
Sbjct: 286 PKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEIS 341

[Close]

BLAST of Lsi08G001690.1 vs. Swiss-Prot
Match: GID1_ORYSJ (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 114/329 (34.65%), Postives = 174/329 (52.89%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     + N+  R + T  R +    D ++P +++P +GV++ D + D +  
Sbjct: 16  VPLHTWVLISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG 75

Query: 64  LWFRVFVPCSD-------------------DSDNLTGLPLLVYFHGGGFSFLAANSMPFV 123
           L  R++   ++                   D+      P++++FHGG F   +A+S  + 
Sbjct: 76  LEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYD 135

Query: 124 DHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDL-----GSDNSDVFPVK 183
             CRR  +  + VVVSVNYR APEH+YP  Y+DG+ ALK++        G D        
Sbjct: 136 SLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQA----- 195

Query: 184 VDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKS 243
               R FL+GDS+GGN+AHHVAVRA     + + + G I +   FGG ER ESE +    
Sbjct: 196 ----RVFLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGK 255

Query: 244 AMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQK 303
             + L+  DWYWKA+LP  +DR+HPA + +GP+   + G+ F  +L+IV G D   D Q 
Sbjct: 256 YFVTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQL 315

Query: 304 RYYEWLKEAGKEVDLVEFPDAVHGFYLVP 309
            Y + L+E G  V +V+  +A  GFYL+P
Sbjct: 316 AYADALREDGHHVKVVQCENATVGFYLLP 332

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BLAST of Lsi08G001690.1 vs. TrEMBL
Match: A0A0A0KUP0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647320 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 6.8e-127
Identity = 225/332 (67.77%), Postives = 269/332 (81.02%), Query Frame = 1


Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           ME++P  FR  L   S A  +S R N TVNRF+M+LFDPK   SSKP  GV+T+DVVFD 
Sbjct: 1   MERLPFKFRILLYFFSFASFISRRSNVTVNRFLMSLFDPKYSASSKPRHGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSD---DSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVV 120
           + NLWFR+F+P S    D++N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV 
Sbjct: 61  SHNLWFRLFLPSSSSSTDNNNVTDLPVIVYYHGGGFVFFSANSMAYDDLCRRLARELRVA 120

Query: 121 VVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLA 180
           VVSVNYRL+PEH+ PIPYEDGFDALK+LD +  D    FPVK+D  RCFLAGDSAGGNLA
Sbjct: 121 VVSVNYRLSPEHRCPIPYEDGFDALKYLDGMDLDGGG-FPVKLDVSRCFLAGDSAGGNLA 180

Query: 181 HHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPH 240
           HHVAVRAGG++FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNLEQADWYWKAFLP 
Sbjct: 181 HHVAVRAGGHNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLEQADWYWKAFLPK 240

Query: 241 GSDRNHPAAHVYGPD-AEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLV 300
           G DRNHPA HV+GP   ++IS VKFP TLLI+GG+DQL DW K+YYEWLK E GKEVDLV
Sbjct: 241 GCDRNHPAVHVFGPSGGDEISKVKFPTTLLILGGKDQLGDWGKKYYEWLKDECGKEVDLV 300

Query: 301 EFPDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           E+P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 EYPNAIHGFYVVPELK-DSSLLIKDMNDFIHK 330

[Close]

BLAST of Lsi08G001690.1 vs. TrEMBL
Match: A0A0A0LUJ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G466560 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.1e-116
Identity = 200/324 (61.73%), Postives = 253/324 (78.09%), Query Frame = 1


Query: 2   EKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDST 61
           +K+PL +R  LR++S   + +LRPN TVNR +++LF+ K+  SS P DGV T D V D +
Sbjct: 12  QKLPLKYRLLLRLRSFVSHKALRPNMTVNRSLISLFESKVSSSSTPRDGVFTCDTVIDPS 71

Query: 62  RNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSV 121
           RNLWFR+FVP S   D    +PLLVYFHGGGF F + +S+PF   CR+LAR+L+ VVVSV
Sbjct: 72  RNLWFRLFVPSSTPHD--LPIPLLVYFHGGGFVFFSPDSLPFDILCRKLARELQAVVVSV 131

Query: 122 NYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVA 181
           NYRL+PEH+YP  YEDGFDALKF+DDL   +S  FP K DF RCF+AGDSAGGN+AHHV 
Sbjct: 132 NYRLSPEHRYPSQYEDGFDALKFIDDL---DSSAFPEKSDFSRCFIAGDSAGGNIAHHVI 191

Query: 182 VRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDR 241
           VR+  Y+FKK+ I+GLIAIQPFFGGEER ESE+++G++  LNLE+ADWYWKAFLP G++R
Sbjct: 192 VRSSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGETPTLNLERADWYWKAFLPDGANR 251

Query: 242 NHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAV 301
           NH AAHV+G     ISGVK PATL+IVGG DQL+DW ++YYEWLK+ GKEV++VE+ +A+
Sbjct: 252 NHVAAHVFGEKGVKISGVKLPATLVIVGGSDQLRDWDRKYYEWLKKGGKEVEMVEYANAI 311

Query: 302 HGFYLVPGLEDDTSLFIKHASEFI 326
           HGFY +P L  +TSL I+ A  FI
Sbjct: 312 HGFYAIPEL-PETSLLIEEAKNFI 329

[Close]

BLAST of Lsi08G001690.1 vs. TrEMBL
Match: A5Y726_CUCME (Esterase OS=Cucumis melo PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.5e-113
Identity = 199/326 (61.04%), Postives = 251/326 (76.99%), Query Frame = 1


Query: 2   EKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDST 61
           +K+PL +R  L + S   +  LRPN TVNR +++LF+ K+  SS P DGV T D V D +
Sbjct: 12  QKLPLKYRLILPLLSFVSHKCLRPNMTVNRSLISLFESKVS-SSSPRDGVFTCDTVIDPS 71

Query: 62  RNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSV 121
           RNLWFR+FVP S   D    +PLL+YFHGGGF F + + + F   CR+LAR+L+ +VVSV
Sbjct: 72  RNLWFRLFVPSSTPHD--LPIPLLIYFHGGGFVFFSPDFLSFDTLCRKLARELQAIVVSV 131

Query: 122 NYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVA 181
           NYRL+PEH+YP  YEDGFDALKF+DDL   +S  FP K DF RCF+AGDSAGGN+AHHV 
Sbjct: 132 NYRLSPEHRYPSQYEDGFDALKFIDDL---DSSAFPKKSDFGRCFIAGDSAGGNIAHHVV 191

Query: 182 VRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDR 241
           VR+  Y+FKK+ I+GLIAIQPFFGGEER ESE+++G+S  LNLE+ADWYWKAFLP G++R
Sbjct: 192 VRSSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGRSPTLNLERADWYWKAFLPDGANR 251

Query: 242 NHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAV 301
           NH AAHV+G D  +IS VKFPATL+IVGG DQL+DW ++YYEWLK+AGKEV+LVE+P A+
Sbjct: 252 NHGAAHVFGEDGVNISAVKFPATLVIVGGSDQLRDWDRKYYEWLKKAGKEVELVEYPKAI 311

Query: 302 HGFYLVPGLEDDTSLFIKHASEFILK 328
           HGFY++  L  +T L I+ A  FI K
Sbjct: 312 HGFYVISEL-PETWLLIEEAKNFIEK 330

[Close]

BLAST of Lsi08G001690.1 vs. TrEMBL
Match: M5WIW5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019404mg PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 4.4e-102
Identity = 184/329 (55.93%), Postives = 235/329 (71.43%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           +P   R  L + +  +N   R N T+NR +M+LFD K  PSSKPN+ V T DV+ D TRN
Sbjct: 6   LPCKVRLLLSLLTIVVNTVRRSNGTINRRLMSLFDLKASPSSKPNNRVKTSDVMVDPTRN 65

Query: 64  LWFRVFVPCSDDSDNLTG----LPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVV 123
           LWFR++VP +  +   T     LPL++YFHGGGF+F +ANS P+ D C+RL+ +L  VVV
Sbjct: 66  LWFRLYVPAAAITTTTTSPSAKLPLIIYFHGGGFAFFSANSKPYDDLCKRLSAELPAVVV 125

Query: 124 SVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHH 183
           SVNYRLAPEH YP  YEDGFD LKF+D    D  D+    VD  RCFLAGDSAGGNLAHH
Sbjct: 126 SVNYRLAPEHIYPSQYEDGFDVLKFIDQTTIDGFDL--NNVDITRCFLAGDSAGGNLAHH 185

Query: 184 VAVRAGGYH-FKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHG 243
           VAV+A  +H F K+ + GLIAIQPFFGG+ER ESE +  K + L+LEQ DWYWK FLP G
Sbjct: 186 VAVKASNHHEFGKMRVVGLIAIQPFFGGKERTESETRLSKGSALSLEQTDWYWKTFLPEG 245

Query: 244 SDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFP 303
           SDR+H AA+V+G +++D+ GV FPAT++ VGG D LQDWQ+RYYE LK++GKE  L+E+P
Sbjct: 246 SDRDHAAANVFGQESKDVLGVNFPATIVFVGGFDLLQDWQRRYYEGLKKSGKEAYLIEYP 305

Query: 304 DAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           ++ HGFY  P L+ + S  +K   +FI K
Sbjct: 306 NSFHGFYAFPELK-EPSFLVKEVRDFIEK 331

[Close]

BLAST of Lsi08G001690.1 vs. TrEMBL
Match: A0A067KH62_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14825 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 4.9e-101
Identity = 181/329 (55.02%), Postives = 235/329 (71.43%), Query Frame = 1


Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           ++PL  R  L   S A + + R N T+NR  + LFDPK P S+KP + V++ D+  D   
Sbjct: 10  RLPLKTRLILATFSLATSTARRSNYTINRNFIKLFDPKAPSSAKPINDVSSFDITIDPNH 69

Query: 63  NLWFRVFVPCSDDS-DNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSV 122
           NLWFR++VP ++ S   +  LP++VYFHGGGF FLAA+S  F   CRRLA+++  VV+SV
Sbjct: 70  NLWFRLYVPATNSSRSTIYSLPVVVYFHGGGFCFLAADSKQFDQFCRRLAKEIPAVVISV 129

Query: 123 NYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVA 182
           NYRLAPE++ P  Y+DGFDALKF+D +  +N   F  KVD + CF+AGDSAGGN+AHHV 
Sbjct: 130 NYRLAPEYKCPSQYDDGFDALKFIDCMNFEN---FSTKVDLEWCFIAGDSAGGNVAHHVT 189

Query: 183 VRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDR 242
           V+AG Y F  L++ GLIAIQPFFGGEER ESE++   S  L+L++ADWYWKAFLP  +DR
Sbjct: 190 VKAGEYEFSNLDVIGLIAIQPFFGGEERTESEMRLTGSPGLSLDRADWYWKAFLPEEADR 249

Query: 243 NHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAV 302
           NHPA +V+GP+  DIS + FPATL+ VGG D LQDWQ +YYEWLK+ GKEV LVE+P+ +
Sbjct: 250 NHPAVNVFGPNGADISELHFPATLVCVGGFDILQDWQMKYYEWLKKCGKEVYLVEYPNVI 309

Query: 303 HGFYLVPGLEDDTSLFIKHASEFILKHMA 331
           HGFY +  L  ++SL I     FI K  A
Sbjct: 310 HGFYCMSEL-PESSLLISEIRTFIRKQTA 334

[Close]

BLAST of Lsi08G001690.1 vs. TAIR10
Match: AT5G23530.1 (AT5G23530.1 carboxyesterase 18)

HSP 1 Score: 304.3 bits (778), Expect = 9.2e-83
Identity = 148/320 (46.25%), Postives = 213/320 (66.56%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           +PL  R  L V S   + + RP+ T+NR  + LFD + PP+ KP + V+T D V D +R+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 64  LWFRVFVP-CSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVN 123
           LWFR++ P  S D      +P++V+FHGGGF+FL+ N+ P+ + CRR AR L   V+SVN
Sbjct: 73  LWFRLYTPHVSGDK-----IPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 124 YRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAV 183
           YRLAPEH+YP  Y+DGFDALK++++   ++  + P   D  RCF AGDSAGGN+AH+VA+
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEE---NHGSILPANADLSRCFFAGDSAGGNIAHNVAI 192

Query: 184 ---RAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 243
              R     F  + + GLI+IQPFFGGEER E+E Q   + +++ ++ DW WKA    G 
Sbjct: 193 RICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GL 252

Query: 244 DRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPD 303
           +R+H A +V GP+A DISG+ +P T+++V G D L+DWQ+ YYEWLK  GK+  L+E+P+
Sbjct: 253 NRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPN 312

Query: 304 AVHGFYLVPGLEDDTSLFIK 320
             H FY+ P L +   L ++
Sbjct: 313 MFHAFYIFPELPEAGQLIMR 321

[Close]

BLAST of Lsi08G001690.1 vs. TAIR10
Match: AT5G27320.1 (AT5G27320.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 242.7 bits (618), Expect = 3.3e-64
Identity = 130/315 (41.27%), Postives = 185/315 (58.73%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A N+  RP+ T NR +    D K+P ++ P +GV + DV+ D   N
Sbjct: 16  VPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTN 75

Query: 64  LWFRVFVPC-SDDSDNLTGL---------PLLVYFHGGGFSFLAANSMPFVDHCRRLARD 123
           L  RV+ P  +  S ++T L         P++V+FHGG F+  +ANS  +   CRRL   
Sbjct: 76  LLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGL 135

Query: 124 LRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAG 183
              VVVSVNYR APE++YP  Y+DG+  LK++    + +S +   K    R FLAGDS+G
Sbjct: 136 CGAVVVSVNYRRAPENRYPCAYDDGWAVLKWV----NSSSWLRSKKDSKVRIFLAGDSSG 195

Query: 184 GNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKA 243
           GN+ H+VAVRA      ++++ G I + P FGG ER ESE +      + +   DWYW+A
Sbjct: 196 GNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRA 255

Query: 244 FLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVD 303
           FLP G DR HPA   +GP ++ + G+ FP +L++V G D +QDWQ +Y E LK+AG+EV 
Sbjct: 256 FLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVK 315

Query: 304 LVEFPDAVHGFYLVP 309
           L+    A  GFYL+P
Sbjct: 316 LLYLEQATIGFYLLP 323

[Close]

BLAST of Lsi08G001690.1 vs. TAIR10
Match: AT3G63010.1 (AT3G63010.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 231.9 bits (590), Expect = 5.8e-61
Identity = 130/339 (38.35%), Postives = 188/339 (55.46%), Query Frame = 1


Query: 4   VPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTRN 63
           VPL     +     A  V  RP+ + NR +    D K+P +S P DGV + D V DST N
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFDHV-DSTTN 75

Query: 64  LWFRVFVPCSDDSDNLTG-------------LPLLVYFHGGGFSFLAANSMPFVDHCRRL 123
           L  R++ P S       G             +P+L++FHGG F+  +ANS  +   CRRL
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 124 ARDLRVVVVSVNYRLAPEHQYPIPYEDGFDALKFLDDL----GSDNSDVFPVKVDFDRCF 183
                VVVVSV+YR +PEH+YP  Y+DG++AL ++          +S+V+         +
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY--------VY 195

Query: 184 LAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQ 243
           LAGDS+GGN+AH+VAVRA     K L   G I + P FGG+ER +SE        + ++ 
Sbjct: 196 LAGDSSGGNIAHNVAVRATNEGVKVL---GNILLHPMFGGQERTQSEKTLDGKYFVTIQD 255

Query: 244 ADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK 303
            DWYW+A+LP G DR+HPA + +GP  + + GV FP +L++V G D +QDWQ  Y + LK
Sbjct: 256 RDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLK 315

Query: 304 EAGKEVDLVEFPDAVHGFYLVPGLEDDTSLFIKHASEFI 326
           + G EV+L+    A  GFY +P   D     ++  ++F+
Sbjct: 316 KTGLEVNLLYLKQATIGFYFLPN-NDHFHCLMEELNKFV 341

[Close]

BLAST of Lsi08G001690.1 vs. TAIR10
Match: AT3G05120.1 (AT3G05120.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 205.3 bits (521), Expect = 5.8e-53
Identity = 105/243 (43.21%), Postives = 147/243 (60.49%), Query Frame = 1


Query: 82  LPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGFDA 141
           +P++++FHGG F+  +ANS  +   CRRL    + VVVSVNYR APE+ YP  Y+DG+ A
Sbjct: 106 VPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIA 165

Query: 142 LKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVAVRAGGYHFKKLNIKGLIAIQ 201
           L +++      S +   K      FLAGDS+GGN+AH+VA+RAG      +++ G I + 
Sbjct: 166 LNWVNS----RSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLN 225

Query: 202 PFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDAEDISGVKF 261
           P FGG ER ESE        + +   DWYWKAFLP G DR HPA + + P  + + GV F
Sbjct: 226 PMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSF 285

Query: 262 PATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAVHGFYLVP------GLEDDTS 319
           P +L++V G D ++DWQ  Y E LK+AG+EV L+    A  GFYL+P       + D+ S
Sbjct: 286 PKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEIS 341

[Close]

BLAST of Lsi08G001690.1 vs. TAIR10
Match: AT5G06570.1 (AT5G06570.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 195.7 bits (496), Expect = 4.6e-50
Identity = 115/293 (39.25%), Postives = 166/293 (56.66%), Query Frame = 1


Query: 21  VSLRPNRTVNRF-IMNLFDPKIPPSSKPNDGVTTHDVVFDSTRNLWFRVFVPCSDDSDNL 80
           + L  N TV R   ++L   +IP   K N  V   D ++    NL  R++ P S  + N 
Sbjct: 18  LQLLSNGTVLRSESIDLITQQIP--FKNNQTVLFKDSIYHKPNNLHLRLYKPIS--ASNR 77

Query: 81  TGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSVNYRLAPEHQYPIPYEDGF 140
           T LP++V+FHGGGF F + +   F + C  LA  L  +VVS +YRLAPEH+ P  +ED  
Sbjct: 78  TALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAE 137

Query: 141 DALKFL-DDLGSDNSDVF---PVKVDFDRCFLAGDSAGGNLAHHVAVR--AGGYHFKKLN 200
             L +L D   SD  + +      VDFDR F+ GDS+GGN+AH +AVR  +G      + 
Sbjct: 138 AVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIELTPVR 197

Query: 201 IKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDRNHPAAHVYGPDA 260
           ++G + + PFFGGEER  SE      A+L+L+  D +W+  LP+G+ R+H  A+ +GP +
Sbjct: 198 VRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPFGPTS 257

Query: 261 EDISGVKFPATLLIVGGQDQLQDWQKRY-YEWLKEAGKEVDLVEFPDAVHGFY 306
             +  +     L+IVGG + L+D  K Y Y+  K  GK VD +EF +  HGFY
Sbjct: 258 PTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHGFY 305

[Close]

BLAST of Lsi08G001690.1 vs. NCBI nr
Match: gi|659119171|ref|XP_008459512.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 474.9 bits (1221), Expect = 1.1e-130
Identity = 230/330 (69.70%), Postives = 271/330 (82.12%), Query Frame = 1


Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           MEK+PL FR  L   S A ++S R N TVNRF M+ FDPK  PSSKP +GV+T+DVVFD 
Sbjct: 1   MEKLPLKFRILLHFHSFASSISRRSNVTVNRFFMSFFDPKFSPSSKPRNGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSDDSD-NLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVV 120
           + NLWFR+F+P S  +D N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV VV
Sbjct: 61  SHNLWFRLFLPSSSSADKNVTDLPVVVYYHGGGFVFFSANSMAYDDFCRRLARELRVAVV 120

Query: 121 SVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHH 180
           SVNYRL+PEH+ PIPYEDGFDALKFLD +  D+   FPVK D  RCFLAGDSAGGNL HH
Sbjct: 121 SVNYRLSPEHRCPIPYEDGFDALKFLDGMDLDDGG-FPVKFDVSRCFLAGDSAGGNLTHH 180

Query: 181 VAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGS 240
           VAVRAGGY+FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNL+Q+DWYWKAFLP G 
Sbjct: 181 VAVRAGGYNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLKQSDWYWKAFLPKGC 240

Query: 241 DRNHPAAHVYGPDA-EDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLVEF 300
           DRNHPAAHV+GP A +DIS VKFP TLLI+GG+DQL DW KRYYEWLK E GKEVDLVE+
Sbjct: 241 DRNHPAAHVFGPGAGDDISKVKFPTTLLILGGKDQLVDWGKRYYEWLKDECGKEVDLVEY 300

Query: 301 PDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 PNAIHGFYIVPELK-DSSLLIKDMNDFIHK 328

[Close]

BLAST of Lsi08G001690.1 vs. NCBI nr
Match: gi|449447535|ref|XP_004141523.1| (PREDICTED: probable carboxylesterase 18 isoform X2 [Cucumis sativus])

HSP 1 Score: 461.8 bits (1187), Expect = 9.8e-127
Identity = 225/332 (67.77%), Postives = 269/332 (81.02%), Query Frame = 1


Query: 1   MEKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDS 60
           ME++P  FR  L   S A  +S R N TVNRF+M+LFDPK   SSKP  GV+T+DVVFD 
Sbjct: 1   MERLPFKFRILLYFFSFASFISRRSNVTVNRFLMSLFDPKYSASSKPRHGVSTYDVVFDP 60

Query: 61  TRNLWFRVFVPCSD---DSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVV 120
           + NLWFR+F+P S    D++N+T LP++VY+HGGGF F +ANSM + D CRRLAR+LRV 
Sbjct: 61  SHNLWFRLFLPSSSSSTDNNNVTDLPVIVYYHGGGFVFFSANSMAYDDLCRRLARELRVA 120

Query: 121 VVSVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLA 180
           VVSVNYRL+PEH+ PIPYEDGFDALK+LD +  D    FPVK+D  RCFLAGDSAGGNLA
Sbjct: 121 VVSVNYRLSPEHRCPIPYEDGFDALKYLDGMDLDGGG-FPVKLDVSRCFLAGDSAGGNLA 180

Query: 181 HHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPH 240
           HHVAVRAGG++FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNLEQADWYWKAFLP 
Sbjct: 181 HHVAVRAGGHNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLEQADWYWKAFLPK 240

Query: 241 GSDRNHPAAHVYGPD-AEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLV 300
           G DRNHPA HV+GP   ++IS VKFP TLLI+GG+DQL DW K+YYEWLK E GKEVDLV
Sbjct: 241 GCDRNHPAVHVFGPSGGDEISKVKFPTTLLILGGKDQLGDWGKKYYEWLKDECGKEVDLV 300

Query: 301 EFPDAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           E+P+A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 301 EYPNAIHGFYVVPELK-DSSLLIKDMNDFIHK 330

[Close]

BLAST of Lsi08G001690.1 vs. NCBI nr
Match: gi|778707738|ref|XP_011656048.1| (PREDICTED: probable carboxylesterase 18 isoform X1 [Cucumis sativus])

HSP 1 Score: 447.2 bits (1149), Expect = 2.5e-122
Identity = 218/329 (66.26%), Postives = 267/329 (81.16%), Query Frame = 1


Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+P   R  +R+ S+A  +S R N TVNRF+++ FDPKIP SSK  +GV+T+DVVFD +R
Sbjct: 7   KLPWKSRVLIRLFSSASFISRRSNITVNRFLISFFDPKIPSSSKQQNGVSTYDVVFDHSR 66

Query: 63  NLWFRVFVPCSDDS--DNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVS 122
           NLWFR F+P S  S  D++T LP+++Y+HGGGF F + +SM + D CRRLAR+L  VV S
Sbjct: 67  NLWFRFFLPFSFSSSADSVT-LPVIIYYHGGGFVFFSPDSMAYDDLCRRLARELGAVVAS 126

Query: 123 VNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHV 182
           VNYRL+PEH++PIPYEDGFDALK+LD +  D    FPVK+D  RCFLAGDSAGGNLAHHV
Sbjct: 127 VNYRLSPEHRFPIPYEDGFDALKYLDGMDLDGGG-FPVKLDVSRCFLAGDSAGGNLAHHV 186

Query: 183 AVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSD 242
           AVRAGG++FKKL IKG+IAIQPFFGGEERVESE+++ KS MLNLEQADWYWKAFLP G D
Sbjct: 187 AVRAGGHNFKKLKIKGIIAIQPFFGGEERVESEIKFSKSPMLNLEQADWYWKAFLPKGCD 246

Query: 243 RNHPAAHVYGPD-AEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLK-EAGKEVDLVEFP 302
           RNHPA HV+GP   ++IS VKFP TLLI+GG+DQL DW K+YYEWLK E GKEVDLVE+P
Sbjct: 247 RNHPAVHVFGPSGGDEISKVKFPTTLLILGGKDQLGDWGKKYYEWLKDECGKEVDLVEYP 306

Query: 303 DAVHGFYLVPGLEDDTSLFIKHASEFILK 328
           +A+HGFY+VP L+ D+SL IK  ++FI K
Sbjct: 307 NAIHGFYVVPELK-DSSLLIKDMNDFIHK 332

[Close]

BLAST of Lsi08G001690.1 vs. NCBI nr
Match: gi|659119173|ref|XP_008459513.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 435.3 bits (1118), Expect = 9.8e-119
Identity = 211/333 (63.36%), Postives = 260/333 (78.08%), Query Frame = 1


Query: 3   KVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDSTR 62
           K+P   R  +R+ S+A  +S R N TVNRF+++ FDPKIP SSKP++GV+T+DVVFD +R
Sbjct: 8   KLPWKSRLLIRLFSSASFISRRSNITVNRFLISFFDPKIPSSSKPHNGVSTYDVVFDHSR 67

Query: 63  NLWFRVFVPCSDDSDNLTG---LPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVV 122
           NLWFR F+P S  S + +    LP++VY+HGGGF F + +SM + D CRRLAR+L  VV 
Sbjct: 68  NLWFRFFLPSSSSSSSSSDGVTLPVVVYYHGGGFVFFSPDSMAYDDLCRRLARELGAVVA 127

Query: 123 SVNYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDV--FPVKVDFDRCFLAGDSAGGNLA 182
           SVNYRL+PEH++PIPYEDGFDALKFLD +  +NSD   FPVK+D  RCFLAGDSAGGNLA
Sbjct: 128 SVNYRLSPEHRFPIPYEDGFDALKFLDTM-DNNSDFGGFPVKLDVSRCFLAGDSAGGNLA 187

Query: 183 HHVAVRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPH 242
           HHVA+R+GGY FKK+ IKGLIAIQP FGGEER  SE+Q+ +S  L LE+ DW WKAFLP+
Sbjct: 188 HHVALRSGGYDFKKVKIKGLIAIQPLFGGEERTNSEIQFKESPTLTLEETDWSWKAFLPN 247

Query: 243 GSDRNHPAAHVYGPD-AEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVE 302
           GS RNHPAAHV+G     D S   FP TLLIVGG+D L+DW++RYYEWLKE GKEVDLVE
Sbjct: 248 GSSRNHPAAHVFGASGVRDTSSATFPPTLLIVGGRDPLRDWERRYYEWLKEGGKEVDLVE 307

Query: 303 FPDAVHGFYLVPGLEDDTSLFIKHASEFILKHM 330
           +P+A+HGFY VP L+ + SL IK A +F+ KHM
Sbjct: 308 YPNAIHGFYAVPELK-ECSLLIKDAIDFMHKHM 338

[Close]

BLAST of Lsi08G001690.1 vs. NCBI nr
Match: gi|778730917|ref|XP_011659884.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 427.6 bits (1098), Expect = 2.0e-116
Identity = 201/324 (62.04%), Postives = 254/324 (78.40%), Query Frame = 1


Query: 2   EKVPLIFRAFLRVQSAALNVSLRPNRTVNRFIMNLFDPKIPPSSKPNDGVTTHDVVFDST 61
           +K+PL +R  LR++S   + +LRPN TVNR +++LF+ K+  SS P DGV T D V D +
Sbjct: 12  QKLPLKYRLLLRLRSFVSHKALRPNMTVNRSLISLFESKVSSSSTPRDGVFTCDTVIDPS 71

Query: 62  RNLWFRVFVPCSDDSDNLTGLPLLVYFHGGGFSFLAANSMPFVDHCRRLARDLRVVVVSV 121
           RNLWFR+FVP S   D    +PLLVYFHGGGF F + +S+PF   CR+LAR+L+ VVVSV
Sbjct: 72  RNLWFRLFVPSSTPHD--LPIPLLVYFHGGGFVFFSPDSLPFDILCRKLARELQAVVVSV 131

Query: 122 NYRLAPEHQYPIPYEDGFDALKFLDDLGSDNSDVFPVKVDFDRCFLAGDSAGGNLAHHVA 181
           NYRL+PEH+YP  YEDGFDALKF+DDL   +S  FP K DF RCF+AGDSAGGN+AHHV 
Sbjct: 132 NYRLSPEHRYPSQYEDGFDALKFIDDL---DSSAFPEKSDFSRCFIAGDSAGGNIAHHVI 191

Query: 182 VRAGGYHFKKLNIKGLIAIQPFFGGEERVESEVQYGKSAMLNLEQADWYWKAFLPHGSDR 241
           VR+  Y+FKK+ I+GLIAIQPFFGGEER ESE+++G++  LNLE+ADWYWKAFLP G++R
Sbjct: 192 VRSSDYNFKKVKIRGLIAIQPFFGGEERTESEIRFGETPTLNLERADWYWKAFLPDGANR 251

Query: 242 NHPAAHVYGPDAEDISGVKFPATLLIVGGQDQLQDWQKRYYEWLKEAGKEVDLVEFPDAV 301
           NH AAHV+G     ISGVKFPATL+IVGG DQL+DW ++YYEWLK+ GKEV++VE+ +A+
Sbjct: 252 NHVAAHVFGEKGVKISGVKFPATLVIVGGSDQLRDWDRKYYEWLKKGGKEVEMVEYANAI 311

Query: 302 HGFYLVPGLEDDTSLFIKHASEFI 326
           HGFY +P L  +TSL I+ A  FI
Sbjct: 312 HGFYAIPEL-PETSLLIEEAKNFI 329

The following BLAST results are available for this feature:

BLAST of Lsi08G001690.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CXE18_ARATH	1.6e-81	46.25	Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1	[more]
GID1C_ARATH	5.8e-63	41.27	Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1	[more]
GID1B_ARATH	1.0e-59	38.35	Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1	[more]
GID1A_ARATH	1.0e-51	43.21	Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1	[more]
GID1_ORYSJ	1.1e-50	34.65	Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1	[more]

BLAST of Lsi08G001690.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUP0_CUCSA	6.8e-127	67.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647320 PE=4 SV=1	[more]
A0A0A0LUJ2_CUCSA	7.1e-116	61.73	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G466560 PE=4 SV=1	[more]
A5Y726_CUCME	2.5e-113	61.04	Esterase OS=Cucumis melo PE=4 SV=1	[more]
M5WIW5_PRUPE	4.4e-102	55.93	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019404mg PE=4 SV=1	[more]
A0A067KH62_JATCU	4.9e-101	55.02	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14825 PE=4 SV=1	[more]

BLAST of Lsi08G001690.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G23530.1	9.2e-83	46.25	carboxyesterase 18	[more]
AT5G27320.1	3.3e-64	41.27	alpha/beta-Hydrolases superfamily protein	[more]
AT3G63010.1	5.8e-61	38.35	alpha/beta-Hydrolases superfamily protein	[more]
AT3G05120.1	5.8e-53	43.21	alpha/beta-Hydrolases superfamily protein	[more]
AT5G06570.1	4.6e-50	39.25	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Lsi08G001690.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659119171\|ref\|XP_008459512.1\|	1.1e-130	69.70	PREDICTED: probable carboxylesterase 18 [Cucumis melo]	[more]
gi\|449447535\|ref\|XP_004141523.1\|	9.8e-127	67.77	PREDICTED: probable carboxylesterase 18 isoform X2 [Cucumis sativus]	[more]
gi\|778707738\|ref\|XP_011656048.1\|	2.5e-122	66.26	PREDICTED: probable carboxylesterase 18 isoform X1 [Cucumis sativus]	[more]
gi\|659119173\|ref\|XP_008459513.1\|	9.8e-119	63.36	PREDICTED: probable carboxylesterase 18 [Cucumis melo]	[more]
gi\|778730917\|ref\|XP_011659884.1\|	2.0e-116	62.04	PREDICTED: probable carboxylesterase 18 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR013094	AB_hydrolase_3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi08G001690	Lsi08G001690	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G001690.1.five_prime_UTR.1	Lsi08G001690.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G001690.1.CDS.1	Lsi08G001690.1.CDS.1	CDS
Lsi08G001690.1.CDS.2	Lsi08G001690.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi08G001690.1.three_prime_UTR.1	Lsi08G001690.1.three_prime_UTR.1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi08G001690.1	Lsi08G001690.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 85..305 score: 5.3
None	No IPR available	PANTHER	PTHR23024	MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES	coord: 1..330 score: 2.9E
None	No IPR available	PANTHER	PTHR23024:SF174	SUBFAMILY NOT NAMED	coord: 1..330 score: 2.9E