Lsi07G008450.1 (mRNA) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi07G008450.1 vs. Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 1.6e-252
Identity = 435/595 (73.11%), Postives = 490/595 (82.35%), Query Frame = 1

Query: 6   SSSWIKSGY---PSSMSQNFSLN-------TNYGTNRLPASCSLHTPSIIQIPKHSHAAF 65
           S  W+   +   P S SQ+  L+        +  T +L  S +LHTP  +  PK S    
Sbjct: 11  SGRWLGGNHTQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQS---- 70

Query: 66  PSPLKTELPKPVPVTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAG 125
            S     + KP      +++ N  QRAAA ALDA E  LVS E+ H LPKTADP+VQIAG
Sbjct: 71  -SNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAG 130

Query: 126 NFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEG 185
           NFAPV EQPVR+ LPV GK+PD I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E 
Sbjct: 131 NFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EH 190

Query: 186 GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGI 245
           G  SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG 
Sbjct: 191 GSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGT 250

Query: 246 GVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVS 305
           GVANAGLV+FNGRLLAMSEDDLPYQV++TP GDLKTVGRFDFDGQL STMIAHPK+DP S
Sbjct: 251 GVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPES 310

Query: 306 GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVF 365
           GE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVF
Sbjct: 311 GELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVF 370

Query: 366 KLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEI 425
           KLPEMIRGGSPV+YDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+E+
Sbjct: 371 KLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEV 430

Query: 426 VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRN 485
           VVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN
Sbjct: 431 VVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRN 490

Query: 486 QLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDD 545
            LGRKT+FAYLALAEPWPKVSGFAKVDL TGE++K++YG+ RYGGEPLFLP EG E ED+
Sbjct: 491 MLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDE 550

Query: 546 GHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQI 590
           G+IL FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI + DL KQ+
Sbjct: 551 GYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of Lsi07G008450.1 vs. Swiss-Prot
Match: NCED1_PHAVU (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=NCED1 PE=2 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 1.6e-252
Identity = 437/617 (70.83%), Postives = 498/617 (80.71%), Query Frame = 1

Query: 1   MATSSSSSWIKSGYPSSMSQNFS----------------LNTNYGTNRLPASC-SLHTPS 60
           M + +S++WI +  PSS S  F                   ++  TN +  S  +LH P 
Sbjct: 1   MPSPASNTWINTTLPSSCSSPFKDLASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPK 60

Query: 61  IIQIPKHSHAAFPSPLK------TELP----KPVPVT--PASEEWNFLQRAAAMALDAVE 120
             Q    S    P+P+K      T  P    KP+  T  P  ++WNFLQ+AAA  LD VE
Sbjct: 61  QYQPTSTSTTTTPTPIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVE 120

Query: 121 NALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPL 180
            ALVS E KH LPKTADP VQIAGNFAPVPE    + LPV GK+P CI GVYVRNGANPL
Sbjct: 121 TALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPL 180

Query: 181 HEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGH 240
           +EPV+GHH FDGDGMVHAV+F+ G   SYACRFTETQRL QE++ GRPVFPKAIGELHGH
Sbjct: 181 YEPVAGHHFFDGDGMVHAVKFTNGAA-SYACRFTETQRLAQEKSLGRPVFPKAIGELHGH 240

Query: 241 SGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKT 300
           SGIARL+LFYAR LF LVD +HG+GVANAGLV+FN  LLAMSEDDLPY VR+T  GDL T
Sbjct: 241 SGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTT 300

Query: 301 VGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLP 360
           VGR+DF+GQL STMIAHPKLDPV+G++ ALSYDV+QKPYLKYF+FS +G KSPDVEIPL 
Sbjct: 301 VGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLK 360

Query: 361 QPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIK 420
           +PTMMHDFAITE FVV+PDQQVVFKL EMI GGSPV+YDK KTSRFGILDKNA DANA++
Sbjct: 361 EPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMR 420

Query: 421 WIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGK 480
           WI+AP+CFCFHLWNAWEEPET+EIVVIGSCMTP DSIFNEC+E+LKSVLSEIRLNL TGK
Sbjct: 421 WIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGK 480

Query: 481 STRRPIITETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYI 540
           STRRPII++ EQVNLEAGMVNRN+LGRKTQFAYLALAEPWPKVSGFAKVDLF+GE++KY+
Sbjct: 481 STRRPIISDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYM 540

Query: 541 YGEQRYGGEPLFLPREGAEAEDDGHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRV 589
           YGE+++GGEPLFLP    E E DG+IL FVHDEKEWKSELQIVNA  L+LEA++KLPSRV
Sbjct: 541 YGEEKFGGEPLFLP--NGEEEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRV 600

BLAST of Lsi07G008450.1 vs. Swiss-Prot
Match: NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 8.4e-238
Identity = 419/596 (70.30%), Postives = 484/596 (81.21%), Query Frame = 1

Query: 2   ATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASC----SLHTPSIIQIPKHSHAAFPS 61
           +TSSSSS  K        Q+ S ++     +L   C    S++  S +     S    P 
Sbjct: 70  STSSSSSRPKL-------QSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPP 129

Query: 62  PLK---TELPKPVP-VTPASEEWNFLQRAAAMALDAVENALVSAERK-HSLPKTADPAVQ 121
             K    ++   +P  T  + + N +QR AAM LDAVENA++S ER+ H  PKTADPAVQ
Sbjct: 130 SWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQ 189

Query: 122 IAGNFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF 181
           IAGNF PVPE+PV   LPVTG VP+CI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F
Sbjct: 190 IAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF 249

Query: 182 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN 241
            + G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD  
Sbjct: 250 -DNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPT 309

Query: 242 HGIGVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLD 301
            G+GVANAGLV+FNG LLAMSEDDLPY V+VT  GDL+T GR+DFDGQL STMIAHPK+D
Sbjct: 310 GGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKID 369

Query: 302 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 361
           P + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQ
Sbjct: 370 PETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQ 429

Query: 362 VVFKLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPET 421
           VVF+LPEMIRGGSPV+YD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN+WEEPET
Sbjct: 430 VVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPET 489

Query: 422 NEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVN 481
           +E+VVIGSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVN
Sbjct: 490 DEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVN 549

Query: 482 RNQLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAE 541
           RN LGRKT++AYLAL EPWPKVSGFAKVDL TGEIRKYIYGE +YGGEPLFLP  G   E
Sbjct: 550 RNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEE 609

Query: 542 DDGHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           D G+I+VFVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL KQ
Sbjct: 610 DGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of Lsi07G008450.1 vs. Swiss-Prot
Match: NCED5_ARATH (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana GN=NCED5 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.3e-234
Identity = 397/577 (68.80%), Postives = 461/577 (79.90%), Query Frame = 1

Query: 15  PSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKHSHAAFPSPLKTELPKPVPVTPASE 74
           PS  S     NT     +L A+    TP+++  P +             P P P+ P  +
Sbjct: 26  PSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-------------PSPNPIIPEKD 85

Query: 75  --EWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVT 134
              WN LQRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APVPEQ V+  L V 
Sbjct: 86  TSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVD 145

Query: 135 GKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQ 194
           GK+PDCI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ +  G  SY+CRFTET+RLVQ
Sbjct: 146 GKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYSCRFTETERLVQ 205

Query: 195 ERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAM 254
           E+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVANAGLV+F+ RLLAM
Sbjct: 206 EKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAM 265

Query: 255 SEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLK 314
           SEDDLPYQVRVT  GDL+T+GRFDFDGQL+S MIAHPK+DPV+ E+FALSYDV++KPYLK
Sbjct: 266 SEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLK 325

Query: 315 YFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKE 374
           YFKFSPEGEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL +M  G SPV YD E
Sbjct: 326 YFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGE 385

Query: 375 KTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNEC 434
           K SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+E+VVIGSCMTP DSIFNEC
Sbjct: 386 KISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNEC 445

Query: 435 EENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWP 494
           +E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGRKT++AYLA+AEPWP
Sbjct: 446 DEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWP 505

Query: 495 KVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILVFVHDEKEWKSEL 554
           KVSGFAKVDL TGE++ + YG ++YGGEP FLPR   ++ EDDG+I+ FVHDE+ W+SEL
Sbjct: 506 KVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESEL 565

Query: 555 QIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
            IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 566 HIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of Lsi07G008450.1 vs. Swiss-Prot
Match: NCED_ONCHC (9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=NCED PE=2 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 4.2e-229
Identity = 386/555 (69.55%), Postives = 448/555 (80.72%), Query Frame = 1

Query: 41  TPSIIQIPKHSHAAFPSPLKTELPKPV----PVTPASEEWNFLQRAAAMALDAVENALVS 100
           TP+   +   S + F  PL    PK +       P+   WN  QRAAA  L+AVE+ L+ 
Sbjct: 57  TPNSFGLDTTSPSPF-YPLPPTCPKEIHPEQSAKPSRPSWNLFQRAAAAVLEAVEDNLIQ 116

Query: 101 --AERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEP 160
              E  H LPKTADPAVQIAGNFAPV EQ     LPV G++P  I GVY+RNGANPL EP
Sbjct: 117 NLLESGHPLPKTADPAVQIAGNFAPVGEQKPHHDLPVDGRIPPLINGVYLRNGANPLFEP 176

Query: 161 VSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGI 220
           V+GHH FDGDGMVHAV     G  SYACRFTET+RL QERA GR +FPKAIGELHGHSGI
Sbjct: 177 VAGHHFFDGDGMVHAVHL-RNGRASYACRFTETERLKQERAVGRAIFPKAIGELHGHSGI 236

Query: 221 ARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGR 280
           ARL+LFYARGL GL+DH+ G GVANAGL++FN RLLAMSEDDLPY VR+ P GDL+T GR
Sbjct: 237 ARLLLFYARGLLGLIDHSRGTGVANAGLIYFNNRLLAMSEDDLPYHVRIKPNGDLETAGR 296

Query: 281 FDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPT 340
           +DFDGQLT+TMIAHPKLDP + E FALSYDVI+KPYLKYF+FSP GEKSPDVEIPLPQPT
Sbjct: 297 YDFDGQLTTTMIAHPKLDPETREFFALSYDVIKKPYLKYFRFSPCGEKSPDVEIPLPQPT 356

Query: 341 MMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIE 400
           MMHDFAIT+ FV+IPDQQVVFKL EMI GGSPV+YDKEK +RFG+L K A DA+ ++WI+
Sbjct: 357 MMHDFAITKNFVIIPDQQVVFKLQEMICGGSPVVYDKEKIARFGVLPKYAIDASEMQWID 416

Query: 401 APDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTR 460
            PDCFCFHLWN+WEEPET E+VVIGSCMTPPDSIFNE EENL+SVL+EIRLNL TGKSTR
Sbjct: 417 VPDCFCFHLWNSWEEPETEEVVVIGSCMTPPDSIFNESEENLQSVLTEIRLNLRTGKSTR 476

Query: 461 RPIITE-TEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYG 520
           RPI+     Q+NLEAGMVNRN+LGR+T+FAYLA+AEPWPKVSGFAKVDL +GEI+++ YG
Sbjct: 477 RPILRPGNSQINLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVDLASGEIQRFEYG 536

Query: 521 EQRYGGEPLFLPREGAEAEDDGHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPY 580
           +  YGGEP F+PREG + ED G++L FVHDE+E  SEL I+NA  + LEA V+LPSRVPY
Sbjct: 537 DGGYGGEPYFVPREGCDREDGGYVLAFVHDEREGSSELLIMNAADMRLEAAVRLPSRVPY 596

Query: 581 GFHGTFISSKDLQKQ 589
           GF+GTF+S+ +L  Q
Sbjct: 597 GFYGTFVSATELHSQ 609

BLAST of Lsi07G008450.1 vs. TrEMBL
Match: K4KBB8_MOMCH (9-cis-epoxycarotenoid dioxygenase OS=Momordica charantia GN=NCED PE=2 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 3.1e-308
Identity = 516/589 (87.61%), Postives = 551/589 (93.55%), Query Frame = 1

Query: 1   MATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKHSHAAFPSPLK 60
           MA++ SS W+KSG+  SM +  S+N+NY  NR+  SCSLHTPSI+QIPKHS    P PLK
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRV--SCSLHTPSIVQIPKHSQTFRPPPLK 60

Query: 61  TELPKPV-PVTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAP 120
           T + KPV P T A E+WNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIAGNFAP
Sbjct: 61  TPVEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAP 120

Query: 121 VPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVS 180
           VPEQPVRK LPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+GGGVS
Sbjct: 121 VPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVS 180

Query: 181 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVAN 240
           YACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HGIGVAN
Sbjct: 181 YACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 240

Query: 241 AGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMF 300
           AGLV+FNGRLLAMSEDDLPYQ+RVTPAGDLKT+GRFDFDGQL STMIAHPKLDPVSGE+F
Sbjct: 241 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELF 300

Query: 301 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360
           A+SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPE
Sbjct: 301 AISYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360

Query: 361 MIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 420
           MIRGGSPVIYDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE+VVIG
Sbjct: 361 MIRGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIG 420

Query: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRK 480
           SCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRK
Sbjct: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRK 480

Query: 481 TQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILV 540
           TQFAYLALAEPWPKVSGFAKVDL TGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHIL 
Sbjct: 481 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILA 540

Query: 541 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 541 FVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of Lsi07G008450.1 vs. TrEMBL
Match: A5BR72_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_018449 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 3.5e-267
Identity = 454/589 (77.08%), Postives = 515/589 (87.44%), Query Frame = 1

Query: 4   SSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKH-SHAAFPSPLKTE 63
           SSSSS   S   SS     S + +  T R   +CSLH+PS++  PKH S +   + +KT 
Sbjct: 25  SSSSS---SSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTT 84

Query: 64  LPKPVP--VTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPV 123
           LPK      +  S++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  LPKEKDDFSSSVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 124 PEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSY 183
           PEQPV+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGDA-SY 204

Query: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 243
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 244 GLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFA 303
           GLV+FN RLLAMSEDDLPYQ+RVTP+GDL+TVGR+DF+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 363
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 364 IRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 423
           I GGSPV+YDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMXWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKT 483
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 484 QFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILV 543
           QFAYLA+AEPWPKVSGFAKVDL TG++ KYIYGEQRYGGEPLFLPR+  +  EDDG+IL 
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 544 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Lsi07G008450.1 vs. TrEMBL
Match: I7FMA3_VITVI (9-cis epoxy carotenoid dioxygenase 3 OS=Vitis vinifera GN=NCED3 PE=2 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 3.5e-267
Identity = 454/589 (77.08%), Postives = 515/589 (87.44%), Query Frame = 1

Query: 4   SSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKH-SHAAFPSPLKTE 63
           SSSSS   S   SS     S + +  T R   +CSLH+PS++  PKH S +   + +KT 
Sbjct: 25  SSSSS---SSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTT 84

Query: 64  LPKPVP--VTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPV 123
           LPK      +  S++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  LPKEKDDFSSSVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 124 PEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSY 183
           PEQPV+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGDA-SY 204

Query: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 243
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 244 GLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFA 303
           GLV+FN RLLAMSEDDLPYQ+RVTP+GDL+TVGR+DF+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 363
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE+FVV+PDQQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPDQQVVFKLQEM 384

Query: 364 IRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 423
           I  GSPV+YDK K SRFG+LDKNATDA+ ++WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISRGSPVVYDKNKMSRFGVLDKNATDASGMRWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKT 483
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 484 QFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILV 543
           QFAYLA+AEPWPKVSGFAKVDL TG++ KYIYGEQRYGGEPLFLPR+  +  EDDG+IL 
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 544 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Lsi07G008450.1 vs. TrEMBL
Match: Q5SGD1_VITVI (9-cis-epoxycarotenoid dioxygenase 1 OS=Vitis vinifera GN=NCED1 PE=2 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 4.6e-267
Identity = 454/589 (77.08%), Postives = 515/589 (87.44%), Query Frame = 1

Query: 4   SSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKH-SHAAFPSPLKTE 63
           SSSSS   S   SS     S + +  T R   +CSLH+PS++  PKH S +   + +KT 
Sbjct: 25  SSSSS---SSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTT 84

Query: 64  LPKPVP--VTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPV 123
           LPK      +  S++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  LPKEKDDFSSSVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 124 PEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSY 183
           PEQPV+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGDA-SY 204

Query: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 243
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 244 GLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFA 303
           GLV+FN RLLAMSEDDLPYQ+RVTP+GDL+TVGR+DF+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 363
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 364 IRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 423
           I GGSPV+YDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMLWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKT 483
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 484 QFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILV 543
           QFAYLA+AEPWPKVSGFAKVDL TG++ KYIYGEQRYGGEPLFLPR+  +  EDDG+IL 
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 544 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609

BLAST of Lsi07G008450.1 vs. TrEMBL
Match: F6HR90_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0093g00550 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 4.6e-267
Identity = 453/591 (76.65%), Postives = 515/591 (87.14%), Query Frame = 1

Query: 2   ATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKH-SHAAFPSPLK 61
           ++SSSSS       SS     S + +  T R   +CSLH+PS++  PKH S +   + +K
Sbjct: 26  SSSSSSS-------SSARDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVK 85

Query: 62  TELPKPVP--VTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 121
           T LPK      +  S +WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFA
Sbjct: 86  TTLPKEKDGFSSSVSPQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFA 145

Query: 122 PVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGV 181
           PVPEQPV   LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F + G  
Sbjct: 146 PVPEQPVHHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKD-GDA 205

Query: 182 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 241
           SYACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVA
Sbjct: 206 SYACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVA 265

Query: 242 NAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 301
           NAGLV+FN RLLAMSEDDLPYQ+RVTP+GDL+TVGR+DF+GQL STMIAHPK+DPVSGEM
Sbjct: 266 NAGLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEM 325

Query: 302 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 361
           FALSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE+FVV+PDQQVVFKL 
Sbjct: 326 FALSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPDQQVVFKLQ 385

Query: 362 EMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 421
           EMI GGSPV+Y+K K SRFG+LDKNATDA+ ++WIEAPDCFCFHLWNAWEEPET+E+VVI
Sbjct: 386 EMISGGSPVVYEKNKMSRFGVLDKNATDASGMRWIEAPDCFCFHLWNAWEEPETDEVVVI 445

Query: 422 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGR 481
           GSCMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGR
Sbjct: 446 GSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGR 505

Query: 482 KTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHI 541
           KTQFAYLA+AEPWPKVSGFAKVDL TG++ KYIYGEQRYGGEPLFLPR+  +  EDDG+I
Sbjct: 506 KTQFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYI 565

Query: 542 LVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           L FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 566 LAFVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 608

BLAST of Lsi07G008450.1 vs. TAIR10
Match: AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)

HSP 1 Score: 873.2 bits (2255), Expect = 8.9e-254
Identity = 435/595 (73.11%), Postives = 490/595 (82.35%), Query Frame = 1

Query: 6   SSSWIKSGY---PSSMSQNFSLN-------TNYGTNRLPASCSLHTPSIIQIPKHSHAAF 65
           S  W+   +   P S SQ+  L+        +  T +L  S +LHTP  +  PK S    
Sbjct: 11  SGRWLGGNHTQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQS---- 70

Query: 66  PSPLKTELPKPVPVTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAG 125
            S     + KP      +++ N  QRAAA ALDA E  LVS E+ H LPKTADP+VQIAG
Sbjct: 71  -SNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAG 130

Query: 126 NFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEG 185
           NFAPV EQPVR+ LPV GK+PD I+GVYVRNGANPLHEPV+GHH FDGDGMVHAV+F E 
Sbjct: 131 NFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EH 190

Query: 186 GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGI 245
           G  SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG 
Sbjct: 191 GSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGT 250

Query: 246 GVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVS 305
           GVANAGLV+FNGRLLAMSEDDLPYQV++TP GDLKTVGRFDFDGQL STMIAHPK+DP S
Sbjct: 251 GVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPES 310

Query: 306 GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVF 365
           GE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVF
Sbjct: 311 GELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVF 370

Query: 366 KLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEI 425
           KLPEMIRGGSPV+YDK K +RFGILDK A D++ IKWI+APDCFCFHLWNAWEEPET+E+
Sbjct: 371 KLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEV 430

Query: 426 VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRN 485
           VVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN
Sbjct: 431 VVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRN 490

Query: 486 QLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDD 545
            LGRKT+FAYLALAEPWPKVSGFAKVDL TGE++K++YG+ RYGGEPLFLP EG E ED+
Sbjct: 491 MLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDE 550

Query: 546 GHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQI 590
           G+IL FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI + DL KQ+
Sbjct: 551 GYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of Lsi07G008450.1 vs. TAIR10
Match: AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)

HSP 1 Score: 824.3 bits (2128), Expect = 4.7e-239
Identity = 419/596 (70.30%), Postives = 484/596 (81.21%), Query Frame = 1

Query: 2   ATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASC----SLHTPSIIQIPKHSHAAFPS 61
           +TSSSSS  K        Q+ S ++     +L   C    S++  S +     S    P 
Sbjct: 70  STSSSSSRPKL-------QSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPP 129

Query: 62  PLK---TELPKPVP-VTPASEEWNFLQRAAAMALDAVENALVSAERK-HSLPKTADPAVQ 121
             K    ++   +P  T  + + N +QR AAM LDAVENA++S ER+ H  PKTADPAVQ
Sbjct: 130 SWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQ 189

Query: 122 IAGNFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF 181
           IAGNF PVPE+PV   LPVTG VP+CI+GVYVRNGANPLH+PVSGHHLFDGDGMVHAV F
Sbjct: 190 IAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF 249

Query: 182 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN 241
            + G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD  
Sbjct: 250 -DNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPT 309

Query: 242 HGIGVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLD 301
            G+GVANAGLV+FNG LLAMSEDDLPY V+VT  GDL+T GR+DFDGQL STMIAHPK+D
Sbjct: 310 GGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKID 369

Query: 302 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 361
           P + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQ
Sbjct: 370 PETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQ 429

Query: 362 VVFKLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPET 421
           VVF+LPEMIRGGSPV+YD++K SRFGIL+KNA DA++I+WIE PDCFCFHLWN+WEEPET
Sbjct: 430 VVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPET 489

Query: 422 NEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVN 481
           +E+VVIGSCMTPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVN
Sbjct: 490 DEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVN 549

Query: 482 RNQLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAE 541
           RN LGRKT++AYLAL EPWPKVSGFAKVDL TGEIRKYIYGE +YGGEPLFLP  G   E
Sbjct: 550 RNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEE 609

Query: 542 DDGHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           D G+I+VFVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS +DL KQ
Sbjct: 610 DGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of Lsi07G008450.1 vs. TAIR10
Match: AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)

HSP 1 Score: 811.2 bits (2094), Expect = 4.1e-235
Identity = 397/577 (68.80%), Postives = 461/577 (79.90%), Query Frame = 1

Query: 15  PSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKHSHAAFPSPLKTELPKPVPVTPASE 74
           PS  S     NT     +L A+    TP+++  P +             P P P+ P  +
Sbjct: 26  PSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNY-------------PSPNPIIPEKD 85

Query: 75  --EWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVT 134
              WN LQRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APVPEQ V+  L V 
Sbjct: 86  TSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVD 145

Query: 135 GKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQ 194
           GK+PDCI GVY+RNGANPL EPVSGHHLFDGDGMVHAV+ +  G  SY+CRFTET+RLVQ
Sbjct: 146 GKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYSCRFTETERLVQ 205

Query: 195 ERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAM 254
           E+  G P+FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVANAGLV+F+ RLLAM
Sbjct: 206 EKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAM 265

Query: 255 SEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLK 314
           SEDDLPYQVRVT  GDL+T+GRFDFDGQL+S MIAHPK+DPV+ E+FALSYDV++KPYLK
Sbjct: 266 SEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLK 325

Query: 315 YFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKE 374
           YFKFSPEGEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL +M  G SPV YD E
Sbjct: 326 YFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGE 385

Query: 375 KTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNEC 434
           K SRFGIL +NA DA+ + W+E+P+ FCFHLWNAWE PET+E+VVIGSCMTP DSIFNEC
Sbjct: 386 KISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNEC 445

Query: 435 EENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWP 494
           +E L SVLSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGRKT++AYLA+AEPWP
Sbjct: 446 DEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWP 505

Query: 495 KVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILVFVHDEKEWKSEL 554
           KVSGFAKVDL TGE++ + YG ++YGGEP FLPR   ++ EDDG+I+ FVHDE+ W+SEL
Sbjct: 506 KVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESEL 565

Query: 555 QIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
            IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 566 HIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of Lsi07G008450.1 vs. TAIR10
Match: AT4G18350.1 (AT4G18350.1 nine-cis-epoxycarotenoid dioxygenase 2)

HSP 1 Score: 761.9 bits (1966), Expect = 2.9e-220
Identity = 362/516 (70.16%), Postives = 433/516 (83.91%), Query Frame = 1

Query: 77  NFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVTGKVP 136
           N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PVPE  VR+ L V G +P
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 137 DCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAY 196
           DCI GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ + G   SYACRFT+T+RLVQE+  
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSA-SYACRFTKTERLVQEKRL 189

Query: 197 GRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDD 256
           GRPVFPKAIGELHGHSGIARLMLFYARGL GL+++ +G+GVANAGLV+FN RLLAMSEDD
Sbjct: 190 GRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDD 249

Query: 257 LPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKF 316
           LPYQ+++T  GDL+TVGR+DFDGQL S MIAHPKLDPV+ E+ ALSYDV++KPYLKYF+F
Sbjct: 250 LPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRF 309

Query: 317 SPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKEKTSR 376
           SP+G KSP++EIPL  PTM+HDFAITE FVVIPDQQVVFKL EMI G SPV++D EK SR
Sbjct: 310 SPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSR 369

Query: 377 FGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENL 436
            GI+ K+AT+A+ I W+ +P+ FCFHLWNAWE PET EIVVIGSCM+P DSIFNE +E+L
Sbjct: 370 LGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESL 429

Query: 437 KSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKTQFAYLALAEPWPKVSG 496
           +SVLSEIR+NL T K+TRR ++   E VNLE GMVNRN+LGRKT+FA+LA+A PWPKVSG
Sbjct: 430 RSVLSEIRINLRTRKTTRRSLLV-NEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 497 FAKVDLFTGEIRKYIYGEQRYGGEPLFLP---REGAEAEDDGHILVFVHDEKEWKSELQI 556
           FAKVDL TGE++KYIYG ++YGGEP FLP     G E EDDG+I   VHDE+   SELQI
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQI 549

Query: 557 VNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQI 590
           +NA+ L+LEAT+KLPSRVPYGFHGTF+ S +L  Q+
Sbjct: 550 INAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQL 583

BLAST of Lsi07G008450.1 vs. TAIR10
Match: AT3G24220.1 (AT3G24220.1 nine-cis-epoxycarotenoid dioxygenase 6)

HSP 1 Score: 653.7 bits (1685), Expect = 1.1e-187
Identity = 314/536 (58.58%), Postives = 404/536 (75.37%), Query Frame = 1

Query: 59  LKTELPKPVPVTPASEEWNFLQRAAAMALDAVENALV-SAERKHSLPKTADPAVQIAGNF 118
           L + +P PV + P     N LQ+ AA  LD +E+++V   E+   LPK  DPAVQ++GNF
Sbjct: 46  LTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNF 105

Query: 119 APVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGG 178
           APV E PV+ GL V G++P C++GVY+RNGANP+  P++GHHLFDGDGM+HAV       
Sbjct: 106 APVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQ 165

Query: 179 VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGV 238
           VSY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD   G+GV
Sbjct: 166 VSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGV 225

Query: 239 ANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGE 298
           ANAG+VFFNGRLLAMSEDDLPYQV++   GDL+T+GRF FD Q+ S++IAHPK+D  +G+
Sbjct: 226 ANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGD 285

Query: 299 MFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKL 358
           +  LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FVVIPDQQ+VFKL
Sbjct: 286 LHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKL 345

Query: 359 PEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE--- 418
            EMIRGGSPVIY KEK +RFG+L K     + I W++ PDCFCFHLWNAWEE  T E   
Sbjct: 346 SEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDP 405

Query: 419 -IVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNR 478
            IVVIGSCM+PPD+IF+E  E  +  LSEIRLN+ T +S R+ I+T    VNLEAG +NR
Sbjct: 406 VIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINR 465

Query: 479 NQLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAED 538
           + +GRK+QF Y+A+A+PWPK SG AKVD+  G + ++ YG  R+GGEP F+P EG   ED
Sbjct: 466 SYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEGEED 525

Query: 539 DGHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQI 590
            G+++ FV DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S   L++Q+
Sbjct: 526 KGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQV 576

BLAST of Lsi07G008450.1 vs. NCBI nr
Match: gi|449460068|ref|XP_004147768.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 538/596 (90.27%), Postives = 563/596 (94.46%), Query Frame = 1

Query: 1   MATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRL-PASCSLHTPSIIQIPKHSHAAFPSPL 60
           MA+SSSSS+++S + SSM       +NY TNRL   S SLHTPSIIQIPKHSH  FPSPL
Sbjct: 1   MASSSSSSFLQSPFSSSMPDKKL--SNYTTNRLLSVSSSLHTPSIIQIPKHSHTTFPSPL 60

Query: 61  KTELPKPVPVTPA-----SEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIA 120
           +T +PKP P+T       S++WNFLQRAAAMALDAVENAL+SAERKHSLPKTADPAVQIA
Sbjct: 61  QTSIPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIA 120

Query: 121 GNFAPVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSE 180
           GNFAPVPEQPVRKGLPV GKVPD IRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE
Sbjct: 121 GNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSE 180

Query: 181 GGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240
            GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG
Sbjct: 181 AGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240

Query: 241 IGVANAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPV 300
           IGVANAGLV+FNGRLLAMSEDDLPYQ+RVTPAGDLKTVGRF+FDGQL STMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVV 360
           SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVV
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVV 360

Query: 361 FKLPEMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE 420
           FKLPEMIRGGSPV+YDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE
Sbjct: 361 FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE 420

Query: 421 IVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480
           +VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN
Sbjct: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480

Query: 481 QLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDD 540
            LGRKTQF+YLALAEPWPKVSGFAKVD+ +GE++KY+YGEQRYGGEPLFLPREGAEAEDD
Sbjct: 481 LLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQRYGGEPLFLPREGAEAEDD 540

Query: 541 GHILVFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQIR 591
           GHIL FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS KDLQKQIR
Sbjct: 541 GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594

BLAST of Lsi07G008450.1 vs. NCBI nr
Match: gi|659131081|ref|XP_008465499.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 540/592 (91.22%), Postives = 560/592 (94.59%), Query Frame = 1

Query: 5   SSSSWIKSGYPSSMSQNFSLNTNYGTNRL-PASCSLHTPSIIQIPKHSHAAFPSPLKTEL 64
           +SSS++KS + SSM       +NY + RL   S SLHTPSIIQIPKHSH  FPSPLKT +
Sbjct: 2   ASSSFLKSPFSSSMPDKTL--SNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSI 61

Query: 65  PKPVPV--TP---ASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 124
           PKP P+  TP    SE+WNFLQRAAAMALDAVENAL+SAERKHSLPKTADPAVQIAGNFA
Sbjct: 62  PKPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA 121

Query: 125 PVPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGV 184
           PVPEQPVRKGLPV GKVP+CIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSE GGV
Sbjct: 122 PVPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGV 181

Query: 185 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 244
           SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVA
Sbjct: 182 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVA 241

Query: 245 NAGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 304
           NAGLV+FNGRLLAMSEDDLPYQ+RVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSGEM
Sbjct: 242 NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 301

Query: 305 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 364
           FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLP
Sbjct: 302 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP 361

Query: 365 EMIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 424
           EMIRGGSPV+YDKEKTSRFGILDKNA DANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI
Sbjct: 362 EMIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 421

Query: 425 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGR 484
           GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRN LGR
Sbjct: 422 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGR 481

Query: 485 KTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHIL 544
           KTQF+YLALAEPWPKVSGFAKVDLFTGEI+KY+YGEQRYGGEPLFLPREGAEAEDDGHIL
Sbjct: 482 KTQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 541

Query: 545 VFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQIR 591
            FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS+DLQKQIR
Sbjct: 542 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 591

BLAST of Lsi07G008450.1 vs. NCBI nr
Match: gi|408794955|gb|AFU91491.1| (9-cis-epoxycarotenoid dioxygenase [Momordica charantia])

HSP 1 Score: 1065.1 bits (2753), Expect = 4.5e-308
Identity = 516/589 (87.61%), Postives = 551/589 (93.55%), Query Frame = 1

Query: 1   MATSSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKHSHAAFPSPLK 60
           MA++ SS W+KSG+  SM +  S+N+NY  NR+  SCSLHTPSI+QIPKHS    P PLK
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRV--SCSLHTPSIVQIPKHSQTFRPPPLK 60

Query: 61  TELPKPV-PVTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAP 120
           T + KPV P T A E+WNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIAGNFAP
Sbjct: 61  TPVEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAP 120

Query: 121 VPEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVS 180
           VPEQPVRK LPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+GGGVS
Sbjct: 121 VPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVS 180

Query: 181 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVAN 240
           YACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HGIGVAN
Sbjct: 181 YACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 240

Query: 241 AGLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMF 300
           AGLV+FNGRLLAMSEDDLPYQ+RVTPAGDLKT+GRFDFDGQL STMIAHPKLDPVSGE+F
Sbjct: 241 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELF 300

Query: 301 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360
           A+SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPE
Sbjct: 301 AISYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 360

Query: 361 MIRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 420
           MIRGGSPVIYDK K SRFGIL KNATDA  IKWIEAPDCFCFHLWNAWEEPETNE+VVIG
Sbjct: 361 MIRGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIG 420

Query: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRK 480
           SCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRK
Sbjct: 421 SCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRK 480

Query: 481 TQFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILV 540
           TQFAYLALAEPWPKVSGFAKVDL TGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHIL 
Sbjct: 481 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILA 540

Query: 541 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 541 FVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

BLAST of Lsi07G008450.1 vs. NCBI nr
Match: gi|1009109982|ref|XP_015893126.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic-like [Ziziphus jujuba])

HSP 1 Score: 938.7 bits (2425), Expect = 4.9e-270
Identity = 458/581 (78.83%), Postives = 512/581 (88.12%), Query Frame = 1

Query: 16  SSMSQNFSLNTNYGTNRLP-ASCSLHTPSIIQIPKHSHAAF-PSPLKTELPKPVPVTPAS 75
           SS S +  L    G  R P  +CSL TPS+I+IPK S + + PS   T   K  P + + 
Sbjct: 8   SSSSSSPFLELGNGKKRRPNINCSLQTPSVIRIPKQSPSTYQPSNTTTIKEKSTPNSVSD 67

Query: 76  -----EEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKG 135
                ++WNFLQ+AAAMAL AVE+ALV  ERKH LPKTADP+VQIAGNFAPV EQPV+  
Sbjct: 68  KPVPFQQWNFLQKAAAMALQAVESALVEHERKHPLPKTADPSVQIAGNFAPVQEQPVQHS 127

Query: 136 LPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQ 195
           LPVTGK+P CI GVYVRNGANP +EPV+GHH FDGDGMVHAV+F + G  SY+CRFTET 
Sbjct: 128 LPVTGKIPKCIEGVYVRNGANPHYEPVAGHHFFDGDGMVHAVQF-KNGTASYSCRFTETH 187

Query: 196 RLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGR 255
           RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARG+FGLVD NHG+GVANAGLV+FNGR
Sbjct: 188 RLVQERAFGRPVFPKAIGELHGHSGIARLLLFYARGIFGLVDSNHGMGVANAGLVYFNGR 247

Query: 256 LLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQK 315
           LLAMSEDDLPY VR+TP+GDLKTVGR+DF+GQL STMIAHPKLDP +GE+FALSYDV+Q+
Sbjct: 248 LLAMSEDDLPYHVRITPSGDLKTVGRYDFNGQLKSTMIAHPKLDPETGELFALSYDVVQR 307

Query: 316 PYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVI 375
           PYLKYF FSP G+KS DVEIPL QPTMMHDFAITE FVVIPDQQVVFKLPEMIRGGSPVI
Sbjct: 308 PYLKYFHFSPSGKKSTDVEIPLSQPTMMHDFAITENFVVIPDQQVVFKLPEMIRGGSPVI 367

Query: 376 YDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSI 435
           YD+EK +RFGILDKNATDA+ IKWIEAPDCFCFHLWNAWEEPE +E+VVIGSCMTPPDSI
Sbjct: 368 YDREKMARFGILDKNATDASGIKWIEAPDCFCFHLWNAWEEPENDEVVVIGSCMTPPDSI 427

Query: 436 FNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKTQFAYLALA 495
           FNECEENLKSVLSEIRLNL TGKSTRRPII+E EQVNLEAGMVNRN+LGRKT+FAYLALA
Sbjct: 428 FNECEENLKSVLSEIRLNLKTGKSTRRPIISEQEQVNLEAGMVNRNRLGRKTRFAYLALA 487

Query: 496 EPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILVFVHDEKEW 555
           EPWPKVSGFAKVDLFTGE+ K++YGEQR+GGEPLFLP++  +E EDDG+IL FVHDEKEW
Sbjct: 488 EPWPKVSGFAKVDLFTGEVNKFMYGEQRFGGEPLFLPKDPNSENEDDGYILAFVHDEKEW 547

Query: 556 KSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           KSELQIVNAMT++LEAT+KLPSRVPYGFHGTFISSKDL+KQ
Sbjct: 548 KSELQIVNAMTMKLEATIKLPSRVPYGFHGTFISSKDLEKQ 587

BLAST of Lsi07G008450.1 vs. NCBI nr
Match: gi|147856958|emb|CAN81383.1| (hypothetical protein VITISV_018449 [Vitis vinifera])

HSP 1 Score: 928.7 bits (2399), Expect = 5.0e-267
Identity = 454/589 (77.08%), Postives = 515/589 (87.44%), Query Frame = 1

Query: 4   SSSSSWIKSGYPSSMSQNFSLNTNYGTNRLPASCSLHTPSIIQIPKH-SHAAFPSPLKTE 63
           SSSSS   S   SS     S + +  T R   +CSLH+PS++  PKH S +   + +KT 
Sbjct: 25  SSSSS---SSSSSSALDLGSFSFHRPTIRPNINCSLHSPSVLHFPKHTSSSTGVATVKTT 84

Query: 64  LPKPVP--VTPASEEWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPV 123
           LPK      +  S++WNFLQ AAAMALDAVE ALV+ E +H LPKTADP VQIAGNFAPV
Sbjct: 85  LPKEKDDFSSSVSQQWNFLQSAAAMALDAVETALVARETQHPLPKTADPRVQIAGNFAPV 144

Query: 124 PEQPVRKGLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSY 183
           PEQPV+  LPV G++PDCI+GVY RNGANPLH+PV+GHHLFDGDGMVHAV+F +G   SY
Sbjct: 145 PEQPVQHSLPVDGRIPDCIQGVYTRNGANPLHKPVAGHHLFDGDGMVHAVQFKDGDA-SY 204

Query: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANA 243
           ACRFTETQRL+QER +GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HG GVANA
Sbjct: 205 ACRFTETQRLIQERDFGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGTGVANA 264

Query: 244 GLVFFNGRLLAMSEDDLPYQVRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFA 303
           GLV+FN RLLAMSEDDLPYQ+RVTP+GDL+TVGR+DF+GQL STMIAHPK+DPVSGEMFA
Sbjct: 265 GLVYFNRRLLAMSEDDLPYQIRVTPSGDLETVGRYDFEGQLRSTMIAHPKVDPVSGEMFA 324

Query: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 363
           LSYDV+QKPYLKYF+FSP+G+KSPDVEIPL +PTMMHDFAITE+FVV+P+QQVVFKL EM
Sbjct: 325 LSYDVVQKPYLKYFRFSPKGDKSPDVEIPLAEPTMMHDFAITERFVVVPNQQVVFKLQEM 384

Query: 364 IRGGSPVIYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGS 423
           I GGSPV+YDK K SRFG+LDKNATDA+ + WIEAPDCFCFHLWNAWEEPET+E+VVIGS
Sbjct: 385 ISGGSPVVYDKNKMSRFGVLDKNATDASGMXWIEAPDCFCFHLWNAWEEPETDEVVVIGS 444

Query: 424 CMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNQLGRKT 483
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTRRPI+ E+EQVNLEAGMVNRN+LGRKT
Sbjct: 445 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRRPILPESEQVNLEAGMVNRNRLGRKT 504

Query: 484 QFAYLALAEPWPKVSGFAKVDLFTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILV 543
           QFAYLA+AEPWPKVSGFAKVDL TG++ KYIYGEQRYGGEPLFLPR+  +  EDDG+IL 
Sbjct: 505 QFAYLAVAEPWPKVSGFAKVDLSTGQVSKYIYGEQRYGGEPLFLPRDPNSGREDDGYILA 564

Query: 544 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 589
           FVHDEK WKSELQIVNA  L+LEA+VKLPSRVPYGFHGTFISSK+L+KQ
Sbjct: 565 FVHDEKTWKSELQIVNATNLQLEASVKLPSRVPYGFHGTFISSKELEKQ 609