Lsi06G005620.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi06G005620.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr06 : 7176065 .. 7180539 (-)
Sequence length	336

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTTAATATATTTAAATTTTTATTTATTTATATTTAATCCAAATTTAAAACGTCAATGACAACCTATATTCCCACCTATAGTTTTGTTTAATTCACTATATATTTTTTAATTATTATTATTTTTTGACATTATCATAGTGTAATAGATGTAATATCTGCCCATAGTTTGTTTTATATATATATATATATATATATATATATATATATAGTGAATAAGTTGTGATCCTCAACCCATGTTATTTACTTTACTAGATTTTTTTATATATTTTTTAAAATGTATCTAGGGTTTAAGGGAGGTTAGATCGGGGAACATAATTCTCGATATTATAGTATTTTATAATGATTTAGTACTAATTTATCAAATATAATAATATGAATTTTTTTAAACTTAAAAAACACACTAGATTTTCTTAAGGAGAATTTTTAAAAATAAAAAAATAAAGGAAACTATTTATATAAAATAGCAAAATTTTTAGATAGTTGTGATAGACGCTGATAGAAACCTATCAGGATCTATCAACAATAGAAATGATAGAAGTCTATCACTGATAGATATGCTGATAGAAGTCTATTACTGATAGATGTGTCTATCAGTATTTTTTTGTTTTTTTCTATAAATAGTTTGACATTTTTTTTATTTGTGAAATTTTTCCATTTCTTAAAAGAAAAGCTGACTAAACATTAATTATTTTAACGGTTACAATAATTTTAAATTAAGTCTGAATTTTGTTAGAAAAATAAAAAAATAAAAAAACCTGAATCTTCCCCTCATTAAGGCTGGAATGCAAATGTTTTGCGATTTTTCCATGCTACTTCCACAATTTTATAACGTAAAAAAAAAAAAAAGTAGAAAATTTTAACAAATCAAAATTTTAAAAACTGTGGTCGGTAAAGTTGCAGAATATTTTAAATTTGATAGGCACAAATATATATAAAGAAATAAAAATTTAAAATATTTAATCACTGTACCTAAAATATTACATGAAAACAAACAAGACATTGACTTCTATTTTTAATTTTTATTTTTGAAATAAACATTGACTTCTATTTTTCCTACATTTGATACTAGACTTCTAGTAATCGGTAATCTATTTCCTATCATGATGTCATTAAATAATATATTATTTTATAGAGAATTTTTAAAAATAGAGGAATGAGAAAAATTATTTATACAAAATTGCAAAATTTTTAGATAGTTGTGATAGATGTCATAGAAGTCTATGAAGGTCTATCAATGATAAAAATGATAGAAGTCTATCACTAATAGATGTTAATAGAAGTCTATCAGTATTTTTTTTTGCTATTTTCTATAAATAATTTGACATTTTTTTATCTGTAAATTTTTTATTTTATTTTATTTTATAAAGCATTATATAATAAAACTTTTAATAAAATAAAAATAAATTAAGTTTATAACATGAAATAATTTATTTATTTATTATTAATAAAAATTTTTTTTACTAGTATATACGTAATGTCATTCCAACATATTGGCCCTACGGTCATTTATAAAATTGAAAATTGCAAACTTCAAGGAAATTGAATTTCTTAACCCAAAAAATACCGTTTTAGAAAAGTATTTGGGAAAAATAGTAATGTTTCCTTATTATTAAAGCTTCTCTATATATATAATTTAGAAAATTATTTGATTAGGTTGTATCCATCTTTTTGTAAATTAGTTGGTCTAACTACAATTTGTTATCACGTGGTAAACTCATAATGATTTTTTTTAAAAATATATTTTTAAACTTTTTCTAATGTGTGATTTAAGTGATATTAAATCAAATGAGTGTCGTCCAAGATATACCCAAATATTGCTCGATATTTAGAAAATTCATGCATACCATTTGTTTGAGTTGCTCTAAAAAGTAACAACTTGGTTGGCAGGCAATCTAGGTTATATTTTATGTTTTCATAATCATTTCAACAAAAAAGAGTTTGGTTAGCTATCTCGATTGTGCTTTTTAAAATTGTTTTTGAATTTTGTGATTTAAATAGTGCAAATTTTGTAAACATCATTTTTAAGTTTTCAATATATCCAAATTTCATATTTAAAATTTTAAAATGCAGAAATATTTTAAAAAAATATAAGGATGTTAATACATAGGGATAATTATAGATGTATATCCTCTACAAAACTTATTTTCATATAAATATCTAAATTTTGGATAAAAAAATATCTAAATTTTGGATATTTTTATTTTGAGGATTTAATTGAAAAATAGAACAAAAATACCCTTTTTCTTAGTAGATGACGTGCATTTATTGCTATTTATTAGGCAAAACTTTTCCATATTTTTCAAACATTCTTAACGATGGAGAGAGAATTTGACCAAATATATATTAATTAGTGTGTTGGAAGGAGAATATACATCTATTATAAATTATTAATTGATTTGAGTTTTTTTTTTCATTTACAACTAATGTGATTTTTTTTCTCATTTTCATGTATGAAACATTTTTTGCTTTTTCTTTCTTCTCGTGGATTTATGGTTATATTTTCAATATACTTTAGAACATTGTTTTAGGTGTTTGAAAATATTCTAACCAATATATGAAATATACCGCAGTAAATAAATGAGAAATGACTCAATGTTCAACATAAATCACAATATAAATCATAAATTTCATTATATCACAGTCTATATAATCTCAATAAGTCAAAATTTAACAACAACTCCTTAACAGAAATGTAATTCAAAATTAATGTAATAACTTCAATGTACCACAGTATATAAATCACTCAACGTTCAACATAAATCACAGTATATATCATAAATTTAATTTATATCACAGTATATATCATAAATTCCATTTAAGTAGTTAGAAATTCCAATATATATCCTATATATTTAGGATTTAAAAAAATAAAAGAGGAAAAAGTATCATCGGTTTGTGTGCATATAACCCTCCCATCTTCTTCTTCCTCGTGAAGCCATTGTCGTCATTCATGCTCTTTGGTTTGACCGTCCGTCGCGCCAACCGTCACGCATCCACCATCGTTTGAGTTGCGCCGTCATTCATAGTCAATTCGAGTCACGAACCGTCCGTCGTTTGTCGTCCTCCTCTCGCGCACCGTCCATCGTCCATTTTCTTTGCACCAGCTTCTTCATCGTCGTCTGCTCGCTAGTGTGGTCACCGGTGAAGATTTTTGTTGTGGATTTTCATTTTGCAGCGGATTTAGGCGGAGGAGGATCTGTGCAGAGGAGGACCTAAGAGAGACGTTGGAGAAGATGAAAAGTGAATGGGTATTTGGGGTTTAATTCAATTTCTTATTTTAAAAAGCATGTAACTAATTGATGGTTGGGGACCGAATGGTAATTGAACCCTAATTTTTGTGTAATTTTAAGCCAACTTAAAGATTTTTGTGTAATTTTAAGCCAACTTAAACATTCCGGTAAATAATAGAAAAGTTGAACATAATTGAAATATAGAGCTTTTATTAGACCTTATGTGTTGTTATCCCTTAGAAATAAGGGATTTTTATATAGAAGGGCTAAAAATAGGTCCATATTACGTTCATATCTTTTACATAAAATAAACTTTCAAATTAATACATGGTTAATACATATGGCTAAATAATCATCCAAAAACTTAACTTAATATTACAAATAATGTTATCCATTCTTTAAACTAATAATATTTATTTTTATTAGATATAAAATTGAATTTTATGTCTAAATAGAAGAACTTCCAAATTCACGTTTGGTGGGTTTTAATTTTATAAACTGTTGAAAAGCTCAAAGGTCATCTATTGACACAATTAAAATTCAAAGGCTCAATCATTATTGGTCATTAGAAGTTACATAGATTTTCAAGCATCAACTTTCTCATGGATATGTTTTTTCTTCTACCAAAAAAGTTTCGTATCAATCAAAATAGTTGCGCATCTCTTATATATATACTCATGATCTTCTCCTTATTTATAATAAACGTGAGACTTTGGTCGCTTTTTCCAACAATGATAAATTAAATATTTTTTTTTTGTCTCTTTACATATGCCACCAATGTATATATATAAACTCATATCCCCTAAAGGATATAGGTATACAAAGCATTCCACTACTACAAATAGTGAGAGTGAACGAGAAAGGGAGTGAGAGAAAACCATGGAAGTTTAATTTCTTTCTTATTCATTAGTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

mRNA sequence

ATGACTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

Coding sequence (CDS)

ATGACTCAATTGGCACACCAAAACTCTCCAGTAGCCATATCAGAAACTTTCATTGTGTTTGTATTTATGGCTTTCATTTCTATGATACTTTTCCAAAGTTATAACCTCAAAAGAAAACACTCAGTTCAACGACAAGCACCTCTCCCACCAGGCCCAAAGCCATGGCCACTCGTAGGTTGCCTCCCAACAATGTTAACCAACAAAATCCCAACACACCAATGGATTCATTCAGTAATGAAGCAATTCAACACTGAAATTGCATCCATACGCCTTGGAAATACTCACATAATCCCTATCACTTCTCCAGAACTCGCTCGGGAATTTCTTAAGACATGA

Protein sequence

MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT

Homology

[Close]

BLAST of Lsi06G005620.1 vs. Swiss-Prot
Match: C79A2_ARATH (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-20
Identity = 49/95 (51.58%), Postives = 62/95 (65.26%), Query Frame = 1


Query: 23  MAFISMILFQSYNLKRKH-------SVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWI 82
           +AFI  +L  +  +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 8   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 67

Query: 83  HSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           HS+MK+ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 68  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILK 102

[Close]

BLAST of Lsi06G005620.1 vs. Swiss-Prot
Match: C79D4_LOTJA (Isoleucine N-monooxygenase 2 OS=Lotus japonicus GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.5e-18
Identity = 48/97 (49.48%), Postives = 65/97 (67.01%), Query Frame = 1


Query: 14  SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQ 73
           S TF++ V   FI   + +S+++ +     ++  LPPGPKPWP+VG LP ML N+ P   
Sbjct: 15  SWTFLLVVIFFFIIFKVTKSHSVNKS----KKYKLPPGPKPWPIVGNLPEMLANR-PATI 74

Query: 74  WIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           WIH +MK+ NTEIA IRL NT +IP+T P +A EFLK
Sbjct: 75  WIHKLMKEMNTEIACIRLANTIVIPVTCPTIACEFLK 106

[Close]

BLAST of Lsi06G005620.1 vs. Swiss-Prot
Match: C79B2_ARATH (Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana GN=CYP79B2 PE=1 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 3.3e-18
Identity = 41/99 (41.41%), Postives = 65/99 (65.66%), Query Frame = 1


Query: 12  AISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPT 71
           + S  +++    AF+++ L            +++  LPPGP  WP++G +PTML ++ P 
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTMLKSR-PV 79

Query: 72  HQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
            +W+HS+MKQ NTEIA ++LGNTH+I +T P++ARE LK
Sbjct: 80  FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILK 117

[Close]

BLAST of Lsi06G005620.1 vs. Swiss-Prot
Match: C79B3_ARATH (Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana GN=CYP79B3 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.1e-17
Identity = 44/89 (49.44%), Postives = 61/89 (68.54%), Query Frame = 1


Query: 22  FMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQ 81
           F+  ++ I   S N K+ H      PLPPGP  +P+VG +P ML N+ P  +W+HS+MK+
Sbjct: 39  FLMILNKIKSSSRN-KKLH------PLPPGPTGFPIVGMIPAMLKNR-PVFRWLHSLMKE 98

Query: 82  FNTEIASIRLGNTHIIPITSPELAREFLK 111
            NTEIA +RLGNTH+IP+T P++ARE  K
Sbjct: 99  LNTEIACVRLGNTHVIPVTCPKIAREIFK 119

[Close]

BLAST of Lsi06G005620.1 vs. Swiss-Prot
Match: C79B1_SINAL (Cytochrome P450 79B1 OS=Sinapis alba GN=CYP79B1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.8e-17
Identity = 41/96 (42.71%), Postives = 61/96 (63.54%), Query Frame = 1


Query: 15  ETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQW 74
           + F+    +  +  +L    N K+         LPPGP  WP++G +PTML ++ P  +W
Sbjct: 31  QAFVAITLVMLLKKVLVNDTNKKK-------LSLPPGPTGWPIIGMVPTMLKSR-PVFRW 90

Query: 75  IHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           +HS+MKQ NTEIA +RLG+TH+I +T P++ARE LK
Sbjct: 91  LHSIMKQLNTEIACVRLGSTHVITVTCPKIAREVLK 118

[Close]

BLAST of Lsi06G005620.1 vs. TrEMBL
Match: A0A0A0LXR0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G620390 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 3.6e-40
Identity = 80/111 (72.07%), Postives = 96/111 (86.49%), Query Frame = 1


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQ+SP+AIS+TFI+F+FMAF S++    YN ++K+SV R+  LPPGPKPWP+VGC
Sbjct: 9   LTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT  WIHSVMKQFNTEIA IRLG+T+IIP+TSPELA EFLKT
Sbjct: 69  LPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. TrEMBL
Match: A0A0A0LXG7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G618390 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 1.3e-34
Identity = 77/113 (68.14%), Postives = 90/113 (79.65%), Query Frame = 1


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           +TQL +QN PVAI S TFI+F+FM+F S   FQ YN   +H+   Q PLPPGPKPWPLVG
Sbjct: 8   ITQLIYQNFPVAITSTTFIIFLFMSFTSFFFFQCYNRIGRHT-NLQPPLPPGPKPWPLVG 67

Query: 61  CLPTMLT-NKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           CLP ML+ N   TH+WIHS+MKQFN EIASIRLGNT++IP+TSPELA EFLKT
Sbjct: 68  CLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLGNTYLIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. TrEMBL
Match: A0A0A0M2W4_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa_1G618890 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 7.3e-33
Identity = 76/113 (67.26%), Postives = 89/113 (78.76%), Query Frame = 1


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           +TQL +QN PVAI S+TFI+F+F++F S   FQ YN   + S   Q PLPPGPKPWPLVG
Sbjct: 8   VTQLIYQNFPVAITSKTFIIFLFISFTSFFFFQRYNHIDRDS-NLQPPLPPGPKPWPLVG 67

Query: 61  CLPTMLT-NKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           CLP ML+ N   TH+WIHS+MKQFN EIASIRL NT+IIP+TSPELA EFLKT
Sbjct: 68  CLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLRNTYIIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. TrEMBL
Match: A0A0A0M089_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G619390 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.3e-25
Identity = 61/90 (67.78%), Postives = 69/90 (76.67%), Query Frame = 1


Query: 23  MAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTML-TNKIPTHQWIHSVMKQ 82
           MAF S++ FQ YN  RKHS   Q PLPPGPK WP VG LP ML +N + TH+WIHS+MKQ
Sbjct: 1   MAFTSILFFQRYNRIRKHS-NLQPPLPPGPKLWPFVGYLPAMLLSNNLSTHEWIHSIMKQ 60

Query: 83  FNTEIASIRLGNTHIIPITSPELAREFLKT 112
           FNTEIASI   NT+IIP+TSPELA EFL T
Sbjct: 61  FNTEIASIHFRNTYIIPVTSPELALEFLHT 89

[Close]

BLAST of Lsi06G005620.1 vs. TrEMBL
Match: A0A0A0KM68_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G223620 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.2e-24
Identity = 62/103 (60.19%), Postives = 74/103 (71.84%), Query Frame = 1


Query: 11  VAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNK-I 70
           +AI+   I+    + I  I F  Y LK   S++R   LPPGPKPWPL+GCLP ML+NK +
Sbjct: 1   MAITFLAIILFIYSIIYAIKFSQYYLKGTPSIRR---LPPGPKPWPLIGCLPAMLSNKNL 60

Query: 71  PTHQWIHSVMKQFNTEIASIRLG-NTHIIPITSPELAREFLKT 112
           P +QWIH VMKQFNTEIA IRLG NTHIIP+ SPEL+ EFL T
Sbjct: 61  PAYQWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNT 100

[Close]

BLAST of Lsi06G005620.1 vs. TAIR10
Match: AT5G05260.1 (AT5G05260.1 cytochrome p450 79a2)

HSP 1 Score: 100.1 bits (248), Expect = 8.8e-22
Identity = 49/95 (51.58%), Postives = 62/95 (65.26%), Query Frame = 1


Query: 23  MAFISMILFQSYNLKRKH-------SVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWI 82
           +AFI  +L  +  +KRK        S  R   LPPGPK WPL+G LP +L    P  +WI
Sbjct: 2   LAFIIGLLLLALTMKRKEKKKTMLISPTRNLSLPPGPKSWPLIGNLPEILGRNKPVFRWI 61

Query: 83  HSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           HS+MK+ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 62  HSLMKELNTDIACIRLANTHVIPVTSPRIAREILK 96

[Close]

BLAST of Lsi06G005620.1 vs. TAIR10
Match: AT5G35917.1 (AT5G35917.1 cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene)

HSP 1 Score: 99.4 bits (246), Expect = 1.5e-21
Identity = 49/96 (51.04%), Postives = 63/96 (65.62%), Query Frame = 1


Query: 23  MAFISMILFQSYNLKRKHSVQ--------RQAPLPPGPKPWPLVGCLPTMLTNKIPTHQW 82
           +AFI  +L  S  +KRK + +        R   LPPGPK WPLVG LP +L    P  +W
Sbjct: 9   LAFILGLLLLSLTMKRKETKKTMLMTSHTRNLSLPPGPKSWPLVGNLPEILGRNKPVFRW 68

Query: 83  IHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
           IHS+M++ NT+IA IRL NTH+IP+TSP +ARE LK
Sbjct: 69  IHSLMEELNTDIACIRLANTHVIPVTSPRIAREILK 104

[Close]

BLAST of Lsi06G005620.1 vs. TAIR10
Match: AT4G39950.1 (AT4G39950.1 cytochrome P450, family 79, subfamily B, polypeptide 2)

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-19
Identity = 41/99 (41.41%), Postives = 65/99 (65.66%), Query Frame = 1


Query: 12  AISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPT 71
           + S  +++    AF+++ L            +++  LPPGP  WP++G +PTML ++ P 
Sbjct: 20  SFSTLYLLSTLQAFVAITLVMLLKKLMTDPNKKKPYLPPGPTGWPIIGMIPTMLKSR-PV 79

Query: 72  HQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLK 111
            +W+HS+MKQ NTEIA ++LGNTH+I +T P++ARE LK
Sbjct: 80  FRWLHSIMKQLNTEIACVKLGNTHVITVTCPKIAREILK 117

[Close]

BLAST of Lsi06G005620.1 vs. TAIR10
Match: AT2G22330.1 (AT2G22330.1 cytochrome P450, family 79, subfamily B, polypeptide 3)

HSP 1 Score: 89.7 bits (221), Expect = 1.2e-18
Identity = 44/89 (49.44%), Postives = 61/89 (68.54%), Query Frame = 1


Query: 22  FMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQ 81
           F+  ++ I   S N K+ H      PLPPGP  +P+VG +P ML N+ P  +W+HS+MK+
Sbjct: 39  FLMILNKIKSSSRN-KKLH------PLPPGPTGFPIVGMIPAMLKNR-PVFRWLHSLMKE 98

Query: 82  FNTEIASIRLGNTHIIPITSPELAREFLK 111
            NTEIA +RLGNTH+IP+T P++ARE  K
Sbjct: 99  LNTEIACVRLGNTHVIPVTCPKIAREIFK 119

[Close]

BLAST of Lsi06G005620.1 vs. TAIR10
Match: AT1G58260.1 (AT1G58260.1 cytochrome p450 79c2)

HSP 1 Score: 70.9 bits (172), Expect = 5.7e-13
Identity = 33/88 (37.50%), Postives = 53/88 (60.23%), Query Frame = 1


Query: 23  MAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGCLPTMLTNKIPTHQWIHSVMKQF 82
           +  IS+ L  +   +    ++ +  LPPGP+ WP+VG +  M+ N+ P H WIH VM++ 
Sbjct: 12  LVLISITLVLALARRFSRFMKPKGQLPPGPRGWPIVGNMLQMIINR-PAHLWIHRVMEEL 71

Query: 83  NTEIASIRLGNTHIIPITSPELAREFLK 111
            T+IA  R    H+I +TS ++ARE L+
Sbjct: 72  QTDIACYRFARFHVITVTSSKIAREVLR 98

[Close]

BLAST of Lsi06G005620.1 vs. NCBI nr
Match: gi|778663781|ref|XP_004151661.2| (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus])

HSP 1 Score: 172.2 bits (435), Expect = 5.2e-40
Identity = 80/111 (72.07%), Postives = 96/111 (86.49%), Query Frame = 1


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQ+SP+AIS+TFI+F+FMAF S++    YN ++K+SV R+  LPPGPKPWP+VGC
Sbjct: 9   LTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT  WIHSVMKQFNTEIA IRLG+T+IIP+TSPELA EFLKT
Sbjct: 69  LPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. NCBI nr
Match: gi|700211432|gb|KGN66528.1| (hypothetical protein Csa_1G620390 [Cucumis sativus])

HSP 1 Score: 172.2 bits (435), Expect = 5.2e-40
Identity = 80/111 (72.07%), Postives = 96/111 (86.49%), Query Frame = 1


Query: 1   MTQLAHQNSPVAISETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVGC 60
           +TQL HQ+SP+AIS+TFI+F+FMAF S++    YN ++K+SV R+  LPPGPKPWP+VGC
Sbjct: 9   LTQLTHQHSPIAISKTFIIFLFMAFASVMFSHYYNRRKKYSVVRKPRLPPGPKPWPIVGC 68

Query: 61  LPTMLTNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           LPTMLTNK PT  WIHSVMKQFNTEIA IRLG+T+IIP+TSPELA EFLKT
Sbjct: 69  LPTMLTNKSPTSHWIHSVMKQFNTEIACIRLGSTYIIPVTSPELAIEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. NCBI nr
Match: gi|700211429|gb|KGN66525.1| (hypothetical protein Csa_1G618390 [Cucumis sativus])

HSP 1 Score: 153.7 bits (387), Expect = 1.9e-34
Identity = 77/113 (68.14%), Postives = 90/113 (79.65%), Query Frame = 1


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           +TQL +QN PVAI S TFI+F+FM+F S   FQ YN   +H+   Q PLPPGPKPWPLVG
Sbjct: 8   ITQLIYQNFPVAITSTTFIIFLFMSFTSFFFFQCYNRIGRHT-NLQPPLPPGPKPWPLVG 67

Query: 61  CLPTMLT-NKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           CLP ML+ N   TH+WIHS+MKQFN EIASIRLGNT++IP+TSPELA EFLKT
Sbjct: 68  CLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLGNTYLIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. NCBI nr
Match: gi|700211430|gb|KGN66526.1| (Cytochrome P450 [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 1.0e-32
Identity = 76/113 (67.26%), Postives = 89/113 (78.76%), Query Frame = 1


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           +TQL +QN PVAI S+TFI+F+F++F S   FQ YN   + S   Q PLPPGPKPWPLVG
Sbjct: 8   VTQLIYQNFPVAITSKTFIIFLFISFTSFFFFQRYNHIDRDS-NLQPPLPPGPKPWPLVG 67

Query: 61  CLPTMLT-NKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
           CLP ML+ N   TH+WIHS+MKQFN EIASIRL NT+IIP+TSPELA EFLKT
Sbjct: 68  CLPAMLSRNNSSTHEWIHSIMKQFNVEIASIRLRNTYIIPVTSPELALEFLKT 119

[Close]

BLAST of Lsi06G005620.1 vs. NCBI nr
Match: gi|778663778|ref|XP_004151554.2| (PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus])

HSP 1 Score: 143.3 bits (360), Expect = 2.6e-31
Identity = 73/113 (64.60%), Postives = 88/113 (77.88%), Query Frame = 1


Query: 1   MTQLAHQNSPVAI-SETFIVFVFMAFISMILFQSYNLKRKHSVQRQAPLPPGPKPWPLVG 60
           + +L +QNSP+AI S+ FI+F+FMAF S++ FQ YN  RKHS   Q PLPPGPK WP VG
Sbjct: 8   INELIYQNSPIAITSKIFILFLFMAFTSILFFQRYNRIRKHS-NLQPPLPPGPKLWPFVG 67

Query: 61  CLPTML-TNKIPTHQWIHSVMKQFNTEIASIRLGNTHIIPITSPELAREFLKT 112
            LP ML +N + TH+WIHS+MKQFNTEIASI   NT+IIP+TSPELA EFL T
Sbjct: 68  YLPAMLLSNNLSTHEWIHSIMKQFNTEIASIHFRNTYIIPVTSPELALEFLHT 119

The following BLAST results are available for this feature:

BLAST of Lsi06G005620.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C79A2_ARATH	1.6e-20	51.58	Phenylalanine N-monooxygenase OS=Arabidopsis thaliana GN=CYP79A2 PE=1 SV=1	[more]
C79D4_LOTJA	2.5e-18	49.48	Isoleucine N-monooxygenase 2 OS=Lotus japonicus GN=CYP79D4 PE=1 SV=1	[more]
C79B2_ARATH	3.3e-18	41.41	Tryptophan N-monooxygenase 1 OS=Arabidopsis thaliana GN=CYP79B2 PE=1 SV=2	[more]
C79B3_ARATH	2.1e-17	49.44	Tryptophan N-monooxygenase 2 OS=Arabidopsis thaliana GN=CYP79B3 PE=1 SV=1	[more]
C79B1_SINAL	2.8e-17	42.71	Cytochrome P450 79B1 OS=Sinapis alba GN=CYP79B1 PE=2 SV=1	[more]

BLAST of Lsi06G005620.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXR0_CUCSA	3.6e-40	72.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G620390 PE=4 SV=1	[more]
A0A0A0LXG7_CUCSA	1.3e-34	68.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G618390 PE=4 SV=1	[more]
A0A0A0M2W4_CUCSA	7.3e-33	67.26	Cytochrome P450 OS=Cucumis sativus GN=Csa_1G618890 PE=4 SV=1	[more]
A0A0A0M089_CUCSA	3.3e-25	67.78	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G619390 PE=4 SV=1	[more]
A0A0A0KM68_CUCSA	6.2e-24	60.19	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G223620 PE=4 SV=1	[more]

BLAST of Lsi06G005620.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G05260.1	8.8e-22	51.58	cytochrome p450 79a2	[more]
AT5G35917.1	1.5e-21	51.04	cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene	[more]
AT4G39950.1	1.8e-19	41.41	cytochrome P450, family 79, subfamily B, polypeptide 2	[more]
AT2G22330.1	1.2e-18	49.44	cytochrome P450, family 79, subfamily B, polypeptide 3	[more]
AT1G58260.1	5.7e-13	37.50	cytochrome p450 79c2	[more]

BLAST of Lsi06G005620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778663781\|ref\|XP_004151661.2\|	5.2e-40	72.07	PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus]	[more]
gi\|700211432\|gb\|KGN66528.1\|	5.2e-40	72.07	hypothetical protein Csa_1G620390 [Cucumis sativus]	[more]
gi\|700211429\|gb\|KGN66525.1\|	1.9e-34	68.14	hypothetical protein Csa_1G618390 [Cucumis sativus]	[more]
gi\|700211430\|gb\|KGN66526.1\|	1.0e-32	67.26	Cytochrome P450 [Cucumis sativus]	[more]
gi\|778663778\|ref\|XP_004151554.2\|	2.6e-31	64.60	PREDICTED: isoleucine N-monooxygenase 1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006570	tyrosine metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0050370	tyrosine N-monooxygenase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi06G005620	Lsi06G005620	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi06G005620.1.CDS.2	Lsi06G005620.1.CDS.2	CDS
Lsi06G005620.1.CDS.1	Lsi06G005620.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi06G005620.1	Lsi06G005620.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 43..110 score: 1.5
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 49..110 score: 2.26
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 17..111 score: 3.2
None	No IPR available	PANTHER	PTHR24298:SF1	CYTOCHROME P450, FAMILY 79, SUBFAMILY C, POLYPEPTIDE 1-RELATED	coord: 17..111 score: 3.2