Lsi06G001480.1 (mRNA) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi06G001480.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionRibosome biogenesis BRX1
Locationchr06 : 1550688 .. 1557046 (+)
Sequence length1703
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGATGCAATTTTGAGCATAATTTTTCAACGAGTCCTATTGAATTGGCAATTTGTAAATTGGTAGCTCCAAAATTTGGTAAATAGGGGGGAGAGTGGTGGGTTTTATCGTCAAATTAGGGTTTTATCGTCACTCTGTGCCGCTTCTCTTTCTCTGTCTGATCAGCACCTTCGTCCTCTTCATCCAGCCACCCGCAAGCAGGCGCCGCCGCTTCATTGCCGATCATCTCCGCCAGTCGACGTTGCTGGGAAGCTTTTGAGTTCTCTTCGTTTCTCTTTTCCTCTCACTCTCGGTTTTTCTTCTCCTTATAGCACCACCGTTCACCGCCCTCAGTGGACGCCGCTCGCCGTTGGTCGTGATCGCCACTGCCGCGGATTCGTACAGCGGACGGGTAAGAATAATCCGTTCAGTTTAAATCACATTACCCATAGCTTGTAGAGTTCAAATGTGTAAGTTCGAACCTATTCGAACACAAACCAGCACGAAATTAACCTCTTTCAGCATAAATTAGTGGGTAAGGACTCATTTTATTAATTTCCAAACTCGTTTTAAGTGCTGTTAGTTAAATTTTGGCCTGTGAAATTCTTCCTTTTGCGGTTTCGAGCTAATTCTGGTTGAATCGACACAGAAGCTTATGGCGTTAATATGGCCAAGTAATAGCATTTAAATTGAGTTTCAAAGTAATTTTGGTGTAACTGTTTTAAGCTTGAAACGCCTTTGAGATTTGTTGTTTGTCTTGCATCATATGATTATATTACGAGGCATCAACTGATTAACTCATGGGACCTCATGCATATGTGCATACGCATAGTCGTTATATTTAGAAGTTATGAGTTCGGAGACTGGAAGATTGCTTCACTTCCCCTTTCAGTGAAGCATATGTTTAATAGAGAGGAAAGCCTTTCGAGTGTGTCGTGTCTGACACAGACACTTCATAAAAAATGAAGTGTATGTGCTTCCTAGCCTTTCACAGGAATGGCTTGGCATTGGTTCCCTTTAGAAACTTAACCCTTCATACCAAATGGTGTTGGAGATTTGCTTAGGAGGGTAAGGCTGGTGGTTAGTATTTATGGGGAAGAACCTCTTGGTTGGAAAGCTACTTTACCTAAAGGGAGCTCATATAATAGGCCCTGGTTTGATATTTGCTAGGATAGTCCCTTTGTTTGAATGATTCTCTAGTTTCATAATTGGTAATGGACAATAAGCTTCTGGAATGATGTATGGTTTGGCTGTTCTCCTCTAAAAGATAATTTTCCAGATATGTGGAGCTTTGGTGGCTGAATGTTGGGATAATGACCTAGAGACTTGGAACCTTAATTTTAGAGGAGGCATTTTTAATAGAAAGGTCAAGAACTGATCGAGTTGACCATGAAACTAGAGGGATTTCGTTTAGGGGCATCCGAGGATAACTTATGTTGGTCTCTTGAACCTTCGGGCTGTTTTTTTTTTTTTACCAAATCCCTCTTCTATAAGCTTGTTAGTGTGAGACATACTCTTCAATGTACGGTTGCTAACCAGATTTGGGGTTCCAGAGTTCCCAATTTTTTTCGTAGGCTCCAAAGAAGAAATCCCTCTCTTGTTCTTGGCCCTTTAGTCTGTCTTTTATGTTGGTGCGTGGAGTTCTTAGATCACATTTTCTTGCATTGTCCCTTTGCAAAACGTGGGCTGGCTGTTTTTTATATTAGAGTTTTCCTTCCCAACAAGGTGGATGATTGGCTGTTAGAATGTGTATTAGAAGTTAGGTCATTTCACTCTCACTCTTTTGTCTTCACCTTTTACAGATTTCTCTAACCTCCATTGCTTAATTTAGGTCCAGTATTTCTAAAGAGGGTGATATTCTCAATGGCAAAGAAGAGGAAGCATAGTGAGATTTTGCCGACAGTATCGGTTAAGAAGGATGACAGCGCTCCTGAGAGACCCAAGCGGACGCTCATGGGTTGGAAAGACAAAGAAGTGGCAAAAGAGTGTGAACCTGGTTGTGATCATGCTGTTTTTCGGAATAAAGAAAAGGTTCTCATCACTTGTTCCCGCCGAATCAATTTTAGGTATGAAATATAGTTGAAACAAGTTGGTGTAGCTTTTTGTAAAATCTATTAATAGGTATATACAGGGTCAGGTAGTTTGTGATGTAAATTTTCACTCACTAACCAAAAGTTCTTTTTTTTAATAGACCAAAAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTAGAATTTCTGGCAGTTTTATTAACATTTGTGATTGATTAGGTATCGGCATTTGATGTTGAATATTGTATCACTTTTGCCACATTGTAAGAAGGATAACAAGGTGGAGTCGAGGTCCAGCAAGGGTGCAACTCTGAATGAGCTGGTTGAGCTCAAGGGTTGTTCTTCCAGCCTATTTTTTGAGGTTAGAACTTTTGCTTCGCACTCTGTCCATGTCTAAACTTGGAATTCAATTCAAAATCTGGAATTTGTAAGGTACAATATTTTTTTAATTTATTTAGGACTATAATTTTTTGGTTTTTGTTGTGGTATGAGGATGTTTTAAGTATACTTTTCTTTTTCATTTTATTATTTTCCTACATGCCTTTTCATCGTGTTTGTACATTGCGATCACATGGCTTATTTTTCCACTTGCTGATATTTTTCTCTCTAATTCCAGTGCAGAAAACATAAAGATCTCTATTTATGGATGGCAAAGTGCCCTAGTGGGCCATCTGTGAAGTTTTTAGTTAATGCCGGTAATTTTAACTTGTATTCAAATTTTATTACTGGGTGATTGAGATTATGTTGGACTTCTTTATTAAATGCAGTTTTCTTACTTTTCCATTTTTGCTTTCAGTGCATACAATGGAAGAATTGAAGCTTACCGGGAACCATCTGAAAGGTTCCCGTCCAATTTTGACATTTTCATCAAATTTTGATAAAGATGTCCACTGGAAACTTCTGAAGGAAATAATTGTTCAGGTATCAATGGCAAAGTTTTTTCTTTGGCAATTCATTGTTGAAACTATTTTCCATTCTTACTCGTGGATCAGTGAATGTATCAAATATCAGTTTCCTTGTCTTTCTTCTTTAAATACTCTTTTTGCTCAGTCGTTTGTTCCTTCAATCCAAAGCGAACATGGTATTAAATTAATAAAATATTGTTAGCACGACTGTGGTCGAATTATCTTTCACTTTTCTTGATGATAGTAAGTTTTTATGAAAAAAAATAAAAAATATTTGTGAATGCATTTCTTTCATGTAATATACTACGCATAAGAATATGGATTAGTGAATACTTGTGCCTGATTTCACATATGCTGGTGCCTCACCACTATCATTCTTTTTTCATGCTGCCTTTATTTATTTCCTATTTAGGGTGGCAATATTAGTTTGATTTGCTGCATATTACTATGTTGTGAAAAGTATGTAGATCTTAGCTGCATCTCCAAATAATGGCTTTTTCGTTTACAAATCCTATGAAGTCTCGTTTTAATGTGACATAATTTTAGCGCAACAACATGTTTTCTCCTCAGATTTTTGGAACTCCGAAGGAGCATCGACATTCTAAACCATATCATGATCATGTATTTGTTTTCTCCATTGTTGATGACCACATCTGGTTCCGGAATTACCAGGTATGCTTTATTTATTTTCTACTTGTTACAGAATTTAATGCTAATCGTGGATGGCATCTAAAAGCACAACCTCCACTTGACACCCAAATAATGCACTGGATGTAGTCTTTCTTTTAAATGTTTGCTCAACGCTTTTTCCAAGAAGTGGCACATGAAATCAGAAAAATATGTTCTACTTTTTGTTAGGCAAAGACATTGGGAAGATTTTTAGGAGTATTGTTAGTATAAATGTCAAATATTTGTTATGATATTGGAAGTATATTAGTCATTAGCTAAGGGTGTTTTAAATGGATGGTTGTTCAGAGAAAGATTAAGATAAGAGCTCGGATGGGTACAAGTAAAGAAACAATCCTCTAACTTCAGATTTAATGACCAAGGTTTCATTCAGAGAAAAAATAAACAATGCTCAGTTTTTAGAGAAAATGACCAAAGCTTCATTCATATAACAAACAATAGGCTTCATTCATATAACAAACAATGGTGGTGAAGTTACTTGACAGGAGATTGCCATTTTATATATGACAAATGCTGGAACCCTCAACTTTTTCGGGGCTGGATAGTAATGAGAGGGTTTACCACTTTATTGAAACATTGTGGGCTGGAGATGATGCATTAAAAAAATTGGAAGAAATGTGATTTGGGTGAATATAAATGCCTTACAATCTCTTCAGCAAAATATGCAAGAACTTTATTCCTTGCTGGATCAATTACATCTTCATTTTTTTTTTCTTTTTTTGGTTTCAAAACAAGAAGTCGGTATCTGAAGTTTGGGTGCAATTTTAAAGCTGTAGCATGAGTAGGAAGTACGGGGGTTCGTTTAACCATCAGCACCCCACCTGAGGGACAATGTGTGCTTTTTGGTGAAGTAAGTTAAAGAATATATGGATAAATGTCAAAAATATCGTCATTAGGATATAAGGGGTACAATTGTCATTAGGTAAGGAATTTGGCATGGTAAAAAATCAATAGGTTGGAGGGGTTTGAGGTTAAGCAGATTTTGGGGAATAGTTTTTGGGGGAGTCCAAGTTTCTCTAGGTAGTTGGGATCTTGTCATTTTTCTTTCTTACGTTCTTGACTTCCATTATTTTTTAGTTCACCGTAGACCATTTTCATATAATTGATCGTTATCCTTTAACTTGCATGTTCACTTTTTGCACCAACTGTTGGTACATTTGTTCTTGTTTGGGGTTTGTGTGGGTGTGATCTATTCTCCCACTGACCATTATTTAATGATATGGTGTTTGTCTTCTTTGTTTGGTGTGATTAGATAGCTGTTCCACATAATGAATCAGATAAAGTCGCCCGAGGAGGACTAGATAAAATGACACTCATTGAGGTTATTAAAATTCCACTTCCTATTCAGTTTATAAATGAGTTTTCACAATTTTCTTGTTGGACTTCAAAGATTTGTATTTTACAGGTTGGCCCACGATTCTGTTTGAATCCAATCAAGATATTTGGTGGAAGTTTTGGAGGCCCTACCCTATATGAGAACCCACTTTATGTATCGCCAAACCAGGTTAGATGATCCAACTTTTTGAACATTTGAGGATTACACTTCTTCGCACTTATCTCTTTTAAACTCGGTTTACAGAATATCTGAGAAACATATGCATTAGTTATCAAGAAGAGAACCACATACAAGATGCATTAGCTATGTTTTCCCGGTTTCTCAAGTTCATATTTTACAAGAGACTAAAAGTATCGAAATTACAGAAAAAATAATGTCGTTGCAAAGGCCAAATATTCATTATCCTTTTCAATTCAAGTTTCAGTAGGATACCTCCTAACAAGAACTCATAAGAACAATAAACAACCCTTAAGGATAGAAATATATATTGCATATATTACTGTAAAAACTACAATATAACATAGCCTTTCGAGAGGGCTACTCTCTGCCAAAATTTCCAAAGGAAATATCAAAAAAAATTTCACACCTTGGTCCTAGCCCACTCCTCTACTTATAACTAAAAGCTCTAACAAACTTACTAACTGTTTACTAATATGTTCCTTTTAATAATCATGCTAATGTTCTACTAATAATTTTGCTATATCTCTAACTAGAGCTGTCATAAATTCTTTGAGATTCCCTTTTGTAACTATTCTCACTTTATTTCAAAAAAAAAAAAAAAAAAAACTATAGTCTCACTTATCTTATGGCCAACCAGAAGTGTCTTTTTGTAATCCATTGGATAGGATTTCATATTTTATAAATTTTGTCAATGTTTCTCCCTTTTCCTAAAAAAAGGAAAGATAAAACCCTCAAATGTGCCCATAGACTTGCTGATGTTCTTCATGTCTCTTCCTAGATTCGAGCATTGGAGAAGAAGCAGAAGGCTGGGAAATACTCGAAGAAAGTCAAAGCGAAAACGAGGAGGAAGATGCACGAGTTATCAAATCCATTGGAACCAGACGAGTTTGCTGATATGTGGAAGGAATAGTATTTCAATTAATTTAATTTTATCTAAGACTTAGAATATAAATTCCTTGCCTCCAGTGAAATGAGATTTTGTTCTATAATAGTAACTGGAGTGCAAATTTTGTCCTTGTTATTTATTTAAACCTTGAGGTATGTTTCTCTATTTTCAATGTATTTCTTAGTTAAATACTTGAGTTCAGATATGATTTTACAAGTTCAAAGAGCATCTCATACTTTTGACTTTATGTTACCCGGAATAGTTCCAAAAGAAAAGAAAAGAAAAGGCGTGTAAGTTGAGAATTTTTTTTTTAAAAAAATTAATTTTAT

mRNA sequence

GATGATGCAATTTTGAGCATAATTTTTCAACGAGTCCTATTGAATTGGCAATTTGTAAATTGGTAGCTCCAAAATTTGGTAAATAGGGGGGAGAGTGGTGGGTTTTATCGTCAAATTAGGGTTTTATCGTCACTCTGTGCCGCTTCTCTTTCTCTGTCTGATCAGCACCTTCGTCCTCTTCATCCAGCCACCCGCAAGCAGGCGCCGCCGCTTCATTGCCGATCATCTCCGCCAGTCGACGTTGCTGGGAAGCTTTTGAGTTCTCTTCGTTTCTCTTTTCCTCTCACTCTCGGTTTTTCTTCTCCTTATAGCACCACCGTTCACCGCCCTCAGTGGACGCCGCTCGCCGTTGGTCGTGATCGCCACTGCCGCGGATTCGTACAGCGGACGGGTCCAGTATTTCTAAAGAGGGTGATATTCTCAATGGCAAAGAAGAGGAAGCATAGTGAGATTTTGCCGACAGTATCGGTTAAGAAGGATGACAGCGCTCCTGAGAGACCCAAGCGGACGCTCATGGGTTGGAAAGACAAAGAAGTGGCAAAAGAGTGTGAACCTGGTTGTGATCATGCTGTTTTTCGGAATAAAGAAAAGGTTCTCATCACTTGTTCCCGCCGAATCAATTTTAGGTATCGGCATTTGATGTTGAATATTGTATCACTTTTGCCACATTGTAAGAAGGATAACAAGGTGGAGTCGAGGTCCAGCAAGGGTGCAACTCTGAATGAGCTGGTTGAGCTCAAGGGTTGTTCTTCCAGCCTATTTTTTGAGTGCAGAAAACATAAAGATCTCTATTTATGGATGGCAAAGTGCCCTAGTGGGCCATCTGTGAAGTTTTTAGTTAATGCCGTGCATACAATGGAAGAATTGAAGCTTACCGGGAACCATCTGAAAGGTTCCCGTCCAATTTTGACATTTTCATCAAATTTTGATAAAGATGTCCACTGGAAACTTCTGAAGGAAATAATTGTTCAGCGCAACAACATGTTTTCTCCTCAGATTTTTGGAACTCCGAAGGAGCATCGACATTCTAAACCATATCATGATCATGTATTTGTTTTCTCCATTGTTGATGACCACATCTGGTTCCGGAATTACCAGATAGCTGTTCCACATAATGAATCAGATAAAGTCGCCCGAGGAGGACTAGATAAAATGACACTCATTGAGGTTGGCCCACGATTCTGTTTGAATCCAATCAAGATATTTGGTGGAAGTTTTGGAGGCCCTACCCTATATGAGAACCCACTTTATGTATCGCCAAACCAGATTCGAGCATTGGAGAAGAAGCAGAAGGCTGGGAAATACTCGAAGAAAGTCAAAGCGAAAACGAGGAGGAAGATGCACGAGTTATCAAATCCATTGGAACCAGACGAGTTTGCTGATATGTGGAAGGAATAGTATTTCAATTAATTTAATTTTATCTAAGACTTAGAATATAAATTCCTTGCCTCCAGTGAAATGAGATTTTGTTCTATAATAGTAACTGGAGTGCAAATTTTGTCCTTGTTATTTATTTAAACCTTGAGGTATGTTTCTCTATTTTCAATGTATTTCTTAGTTAAATACTTGAGTTCAGATATGATTTTACAAGTTCAAAGAGCATCTCATACTTTTGACTTTATGTTACCCGGAATAGTTCCAAAAGAAAAGAAAAGAAAAGGCGTGTAAGTTGAGAATTTTTTTTTTAAAAAAATTAATTTTAT

Coding sequence (CDS)

ATGGCAAAGAAGAGGAAGCATAGTGAGATTTTGCCGACAGTATCGGTTAAGAAGGATGACAGCGCTCCTGAGAGACCCAAGCGGACGCTCATGGGTTGGAAAGACAAAGAAGTGGCAAAAGAGTGTGAACCTGGTTGTGATCATGCTGTTTTTCGGAATAAAGAAAAGGTTCTCATCACTTGTTCCCGCCGAATCAATTTTAGGTATCGGCATTTGATGTTGAATATTGTATCACTTTTGCCACATTGTAAGAAGGATAACAAGGTGGAGTCGAGGTCCAGCAAGGGTGCAACTCTGAATGAGCTGGTTGAGCTCAAGGGTTGTTCTTCCAGCCTATTTTTTGAGTGCAGAAAACATAAAGATCTCTATTTATGGATGGCAAAGTGCCCTAGTGGGCCATCTGTGAAGTTTTTAGTTAATGCCGTGCATACAATGGAAGAATTGAAGCTTACCGGGAACCATCTGAAAGGTTCCCGTCCAATTTTGACATTTTCATCAAATTTTGATAAAGATGTCCACTGGAAACTTCTGAAGGAAATAATTGTTCAGCGCAACAACATGTTTTCTCCTCAGATTTTTGGAACTCCGAAGGAGCATCGACATTCTAAACCATATCATGATCATGTATTTGTTTTCTCCATTGTTGATGACCACATCTGGTTCCGGAATTACCAGATAGCTGTTCCACATAATGAATCAGATAAAGTCGCCCGAGGAGGACTAGATAAAATGACACTCATTGAGGTTGGCCCACGATTCTGTTTGAATCCAATCAAGATATTTGGTGGAAGTTTTGGAGGCCCTACCCTATATGAGAACCCACTTTATGTATCGCCAAACCAGATTCGAGCATTGGAGAAGAAGCAGAAGGCTGGGAAATACTCGAAGAAAGTCAAAGCGAAAACGAGGAGGAAGATGCACGAGTTATCAAATCCATTGGAACCAGACGAGTTTGCTGATATGTGGAAGGAATAG

Protein sequence

MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKAKTRRKMHELSNPLEPDEFADMWKE
Homology
[Close]
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match: BRX1_XENLA (Ribosome biogenesis protein BRX1 homolog OS=Xenopus laevis GN=brix1 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-64
Identity = 141/323 (43.65%), Postives = 195/323 (60.37%), Query Frame = 1 

Query: 2   AKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKE---CEPGCDHAVFRNKEKVL 61
           A KRK    LP V+        ++ K+ L G + +  A E     P      ++NKE+VL
Sbjct: 3   AYKRKRGS-LPEVATNT-----KKAKKQLAGSEQEATAGEEFIVPPPVSEGKWKNKERVL 62

Query: 62  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 121
           I  SR INFR RHLM ++ SL+PH + + K++ R  K   +NE+ E+K C+  ++FE +K
Sbjct: 63  IFSSRGINFRTRHLMQDLRSLMPHSRAETKMD-RKDKLFVVNEVCEMKNCNKCIYFEAKK 122

Query: 122 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 181
            +DLY+W++  P GPS KFLV  +HT+ ELK++GN LKGSRPIL+F   FD++  + LLK
Sbjct: 123 KQDLYMWLSNSPEGPSAKFLVQNIHTLAELKMSGNCLKGSRPILSFDPAFDREPQYALLK 182

Query: 182 EIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVAR 241
           E++         QIFGTP+ H  S+P+ DH+F FSI D+ IWFRNYQI     E D    
Sbjct: 183 ELLT--------QIFGTPRYHPRSQPFVDHIFTFSIADNRIWFRNYQII----EED---- 242

Query: 242 GGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKV 301
                  L+E+GPRF LN IKIF GSFGGPTLYENP Y SPN  R + +   A K  +K 
Sbjct: 243 -----AALVEIGPRFVLNLIKIFKGSFGGPTLYENPHYQSPNMHRRMIRLATAAKVKEKQ 297

Query: 302 KAKTRRKMHELSN----PLEPDE 318
           + K  +K+ +  +    P++P E
Sbjct: 303 QVKEVQKLKKEEDRPVIPVDPTE 297

[Close]
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match: BRX1_HUMAN (Ribosome biogenesis protein BRX1 homolog OS=Homo sapiens GN=BRIX1 PE=1 SV=2)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 142/323 (43.96%), Postives = 195/323 (60.37%), Query Frame = 1 

Query: 2   AKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLITC 61
           AKK K +EI        D   P +   T    +++E  +   P C    ++NKE++LI  
Sbjct: 16  AKKPKRNEI--------DAEPPAKRHATAEEVEEEERDRIPGPVCK-GKWKNKERILIFS 75

Query: 62  SRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHKD 121
           SR INFR RHLM ++  L+PH K D K++ R  K   +NE+ E+K C+  ++FE +K +D
Sbjct: 76  SRGINFRTRHLMQDLRMLMPHSKADTKMD-RKDKLFVINEVCEMKNCNKCIYFEAKKKQD 135

Query: 122 LYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEII 181
           LY+W++  P GPS KFLV  +HT+ ELK+TGN LKGSRP+L+F   FD+  H+ LLKE++
Sbjct: 136 LYMWLSNSPHGPSAKFLVQNIHTLAELKMTGNCLKGSRPLLSFDPAFDELPHYALLKELL 195

Query: 182 VQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGGL 241
           +        QIF TP+ H  S+P+ DHVF F+I+D+ IWFRN+QI     E D       
Sbjct: 196 I--------QIFSTPRYHPKSQPFVDHVFTFTILDNRIWFRNFQII----EED------- 255

Query: 242 DKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKAK 301
               L+E+GPRF LN IKIF GSFGGPTLYENP Y SPN  R + +   A KY +K + K
Sbjct: 256 --AALVEIGPRFVLNLIKIFQGSFGGPTLYENPHYQSPNMHRRVIRSITAAKYREKQQVK 307

Query: 302 TRRKMH--ELSNPLEPDEFADMW 323
             +K+   E    L  D  AD++
Sbjct: 316 DVQKLRKKEPKTLLPHDPTADVF 307

[Close]
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match: BRX1_RAT (Ribosome biogenesis protein BRX1 homolog OS=Rattus norvegicus GN=Brix1 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-64
Identity = 137/298 (45.97%), Postives = 182/298 (61.07%), Query Frame = 1 

Query: 34  KDKEVAKECEPGCDH-------AVFRNKEKVLITCSRRINFRYRHLMLNIVSLLPHCKKD 93
           K  +VAKE E   D          ++NKE++LI  SR INFR RHLM ++  L+PH K D
Sbjct: 31  KQADVAKEAEEEKDRIPGPVCKGKWKNKERILIFSSRGINFRTRHLMQDLRMLMPHSKAD 90

Query: 94  NKVESRSSKGATLNELVELKGCSSSLFFECRKHKDLYLWMAKCPSGPSVKFLVNAVHTME 153
            K++ R  K   +NE+ E+K C+  ++FE +K +DLY+W++  P GPS KFLV  +HT+ 
Sbjct: 91  TKMD-RKDKLFVINEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPHGPSAKFLVQNIHTLA 150

Query: 154 ELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEIIVQRNNMFSPQIFGTPKEHRHSKPYH 213
           ELK+TGN LKGSRP+L+F   FD   H+ LLKE ++        QIF TP+ H  S+P+ 
Sbjct: 151 ELKMTGNCLKGSRPLLSFDPAFDDLPHYALLKEFLI--------QIFSTPRYHPKSQPFV 210

Query: 214 DHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGGLDKMTLIEVGPRFCLNPIKIFGGSFG 273
           DHVF F+I+D+ IWFRN+QI     E D           L+E+GPRF LN IKIF GSFG
Sbjct: 211 DHVFTFTILDNRIWFRNFQII----EED---------AALVEIGPRFVLNLIKIFQGSFG 270

Query: 274 GPTLYENPLYVSPNQIRALEKKQKAGKYSKK--VKAKTRRKMHELSNPLEPDEFADMW 323
           GPTLYENP Y SPN  R + +   A KY +K  VK   + +  E    L  D  AD++
Sbjct: 271 GPTLYENPHYQSPNMHRRVVRSITAAKYREKQQVKDVQKSRKKEPKTILPHDPTADVF 306

[Close]
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match: BRX1_PONAB (Ribosome biogenesis protein BRX1 homolog OS=Pongo abelii GN=BRIX1 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.2e-64
Identity = 141/323 (43.65%), Postives = 194/323 (60.06%), Query Frame = 1 

Query: 2   AKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLITC 61
           AKK K +E        KD   P +   T    +++E  +   P C    ++NKE++LI  
Sbjct: 16  AKKPKRNE--------KDAEPPAKRHATAEEVEEEERDRIPGPVCK-GKWKNKERILIFS 75

Query: 62  SRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHKD 121
           SR INFR RHLM ++  L+PH K D K++ R  K   +NE+ E+K C+  ++FE +K +D
Sbjct: 76  SRGINFRTRHLMQDLRMLMPHSKADTKMD-RKDKLFVINEVCEMKNCNKCIYFEAKKKQD 135

Query: 122 LYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEII 181
           LY+W++  P GPS KFLV  +HT+ ELK+TGN LKGSRP+L+F   FD+  H+ LLKE++
Sbjct: 136 LYMWLSNSPHGPSAKFLVQNIHTLAELKMTGNCLKGSRPLLSFDPAFDELPHYALLKELL 195

Query: 182 VQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGGL 241
           +        QI  TP+ H  S+P+ DHVF F+I+D+ IWFRN+QI     E D       
Sbjct: 196 I--------QILSTPRYHPKSQPFVDHVFTFTILDNRIWFRNFQII----EED------- 255

Query: 242 DKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKAK 301
               L+E+GPRF LN IKIF GSFGGPTLYENP Y SPN  R + +   A KY +K + K
Sbjct: 256 --AALVEIGPRFVLNLIKIFQGSFGGPTLYENPHYQSPNMHRRVTRSITAAKYREKQQVK 307

Query: 302 TRRKMH--ELSNPLEPDEFADMW 323
             +K+   E    L  D  AD++
Sbjct: 316 DVQKLRKKEPKTLLPHDPTADVF 307

[Close]
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match: BRX1_MOUSE (Ribosome biogenesis protein BRX1 homolog OS=Mus musculus GN=Brix1 PE=1 SV=3)

HSP 1 Score: 246.1 bits (627), Expect = 5.2e-64
Identity = 137/300 (45.67%), Postives = 184/300 (61.33%), Query Frame = 1 

Query: 34  KDKEVAKECE---------PGCDHAVFRNKEKVLITCSRRINFRYRHLMLNIVSLLPHCK 93
           K  +VAKE E         P C    ++NKE++LI  SR INFR RHLM ++  L+PH K
Sbjct: 31  KQADVAKEAEEENRDRIPGPVCK-GKWKNKERILIFSSRGINFRTRHLMQDLRMLMPHSK 90

Query: 94  KDNKVESRSSKGATLNELVELKGCSSSLFFECRKHKDLYLWMAKCPSGPSVKFLVNAVHT 153
            D K++ R  K   +NE+ E+K C+  ++FE +K +DLY+W++  P GPS KFLV  +HT
Sbjct: 91  ADTKMD-RKDKLFVINEVCEMKNCNKCIYFEAKKKQDLYMWLSNSPHGPSAKFLVQNIHT 150

Query: 154 MEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEIIVQRNNMFSPQIFGTPKEHRHSKP 213
           + ELK+TGN LKGSRP+L+F   FD   H+ LLKE ++        QIF TP+ H  S+P
Sbjct: 151 LAELKMTGNCLKGSRPLLSFDPAFDDLPHYALLKEFLI--------QIFSTPRYHPKSQP 210

Query: 214 YHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGGLDKMTLIEVGPRFCLNPIKIFGGS 273
           + DHVF F+I+D+ IWFRN+QI     E D           L+E+GPRF LN IKIF GS
Sbjct: 211 FVDHVFTFTILDNRIWFRNFQII----EED---------AALVEIGPRFVLNLIKIFQGS 270

Query: 274 FGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKAKTRRKMH--ELSNPLEPDEFADMW 323
           FGGPTLYENP Y SPN  R + +   A KY ++ + K  +K+   E    L  D  AD++
Sbjct: 271 FGGPTLYENPHYQSPNMHRRVIRSITAAKYRERQQVKDVQKLRKKEPKTILPHDPTADVF 307

[Close]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match: A0A0D2SNA9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G319600 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 2.5e-158
Identity = 271/330 (82.12%), Postives = 298/330 (90.30%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECEPGCDHA-VFRNKEKVL 60
           M KKRKHSE       KKD++APERPKR+L GWKDKE V ++ E   D   VFRNKEKV+
Sbjct: 1   MGKKRKHSETQTAEPKKKDEAAPERPKRSLFGWKDKEEVQQQTESNTDPPQVFRNKEKVM 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNEL+EL+GCSS+LFFECRK
Sbjct: 61  VTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELIELRGCSSALFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           H+DLYLWMA+CP+GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+KD HWKLLK
Sbjct: 121 HQDLYLWMARCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAHWKLLK 180

Query: 181 EIIVQRNNMFSP----QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESD 240
           E+I+Q ++ F      QIFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESD
Sbjct: 181 EMIIQFSDTFFSIIFFQIFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESD 240

Query: 241 KVARGGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKY 300
           K+ RGGLDKMTL+EVGPRFCLNPIKIF GSFGGPTLYENP YVSPNQIRALEK+QKAGKY
Sbjct: 241 KMVRGGLDKMTLVEVGPRFCLNPIKIFAGSFGGPTLYENPFYVSPNQIRALEKRQKAGKY 300

Query: 301 SKKVKAKTRRKMHELSNPLEPDEFADMWKE 325
           +KKVKAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 AKKVKAKTRRKMHELSNPLEPDEFADMWRE 330

[Close]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match: A0A0D2PI01_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G319600 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 3.6e-157
Identity = 269/326 (82.52%), Postives = 294/326 (90.18%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECEPGCDHA-VFRNKEKVL 60
           M KKRKHSE       KKD++APERPKR+L GWKDKE V ++ E   D   VFRNKEKV+
Sbjct: 1   MGKKRKHSETQTAEPKKKDEAAPERPKRSLFGWKDKEEVQQQTESNTDPPQVFRNKEKVM 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNEL+EL+GCSS+LFFECRK
Sbjct: 61  VTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELIELRGCSSALFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           H+DLYLWMA+CP+GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+KD HWKLLK
Sbjct: 121 HQDLYLWMARCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAHWKLLK 180

Query: 181 EIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVAR 240
           E+I+Q        IFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESDK+ R
Sbjct: 181 EMIIQ--------IFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESDKMVR 240

Query: 241 GGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKV 300
           GGLDKMTL+EVGPRFCLNPIKIF GSFGGPTLYENP YVSPNQIRALEK+QKAGKY+KKV
Sbjct: 241 GGLDKMTLVEVGPRFCLNPIKIFAGSFGGPTLYENPFYVSPNQIRALEKRQKAGKYAKKV 300

Query: 301 KAKTRRKMHELSNPLEPDEFADMWKE 325
           KAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 KAKTRRKMHELSNPLEPDEFADMWRE 318

[Close]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match: A0A0B0NEE6_GOSAR (Ribosome biogenesis BRX1 OS=Gossypium arboreum GN=F383_18642 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 3.6e-157
Identity = 269/326 (82.52%), Postives = 294/326 (90.18%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECEPGCDHA-VFRNKEKVL 60
           M KKRKHSE       KKD++APERPKR+L GWKDKE V ++ E   D   VFRNKEKV+
Sbjct: 1   MGKKRKHSETQTAEPKKKDEAAPERPKRSLFGWKDKEEVQQQTESNTDPPQVFRNKEKVM 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNEL+EL+GCSS+LFFECRK
Sbjct: 61  VTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELIELRGCSSALFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           H+DLYLWMA+CP+GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+KD HWKLLK
Sbjct: 121 HQDLYLWMARCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAHWKLLK 180

Query: 181 EIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVAR 240
           E+I+Q        IFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESDK+ R
Sbjct: 181 EMIIQ--------IFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESDKMVR 240

Query: 241 GGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKV 300
           GGLDKMTL+EVGPRFCLNPIKIF GSFGGPTLYENP YVSPNQIRALEK+QKAGKY+KKV
Sbjct: 241 GGLDKMTLVEVGPRFCLNPIKIFAGSFGGPTLYENPFYVSPNQIRALEKRQKAGKYAKKV 300

Query: 301 KAKTRRKMHELSNPLEPDEFADMWKE 325
           KAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 KAKTRRKMHELSNPLEPDEFADMWRE 318

[Close]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match: A0A061F737_THECC (Ribosome biogenesis protein brix, putative OS=Theobroma cacao GN=TCM_031378 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-156
Identity = 271/327 (82.87%), Postives = 291/327 (88.99%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECE--PGCDHAVFRNKEKV 60
           M KKRK SE        KD+ APERPKRTL+GWKDKE VAK+ E  P     VFRNKEKV
Sbjct: 1   MGKKRKQSETQTAEPTNKDEGAPERPKRTLVGWKDKEEVAKQTESNPDSSSQVFRNKEKV 60

Query: 61  LITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECR 120
           ++TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVES+SSKGATLNEL+EL GCSS LFFECR
Sbjct: 61  MVTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESKSSKGATLNELIELSGCSSCLFFECR 120

Query: 121 KHKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLL 180
           KH+DLYLWMAK PSGPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFS NF+KD HWKLL
Sbjct: 121 KHQDLYLWMAKSPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSGNFEKDAHWKLL 180

Query: 181 KEIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVA 240
           KE+I+Q        IFGTPKEHR SKPYHDHVFVFSIVDDHIWFRNYQI+VPHNESDK+ 
Sbjct: 181 KEMIMQ--------IFGTPKEHRKSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKMV 240

Query: 241 RGGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKK 300
           RGGLDKMTL+EVGPRFCLNPIKIFGGSFGGPTLYENP YVSPNQIRALEK+QKAGKY+KK
Sbjct: 241 RGGLDKMTLVEVGPRFCLNPIKIFGGSFGGPTLYENPFYVSPNQIRALEKRQKAGKYAKK 300

Query: 301 VKAKTRRKMHELSNPLEPDEFADMWKE 325
           VKAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 VKAKTRRKMHELSNPLEPDEFADMWRE 319

[Close]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match: A0A067JSR7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_22878 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 5.2e-156
Identity = 273/325 (84.00%), Postives = 288/325 (88.62%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDK-EVAKECEPGCDHAVFRNKEKVLI 60
           M KKRK SE   T   KKD+SAPERP+RTL+GWKD     KE E      VFRNKEKVL+
Sbjct: 1   MGKKRKISETEVTAPSKKDESAPERPQRTLLGWKDNGSEVKETET----QVFRNKEKVLV 60

Query: 61  TCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKH 120
           TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVES+SSKG  LNELVELK CSS LFFECRKH
Sbjct: 61  TCSRRINYRYRHLMLNVVSLLPHCKKDNKVESKSSKGMALNELVELKSCSSCLFFECRKH 120

Query: 121 KDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKE 180
           KDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNFDKD HWKLLKE
Sbjct: 121 KDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFDKDDHWKLLKE 180

Query: 181 IIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARG 240
           +I+Q        IFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESDKVARG
Sbjct: 181 MIIQ--------IFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESDKVARG 240

Query: 241 GLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVK 300
           GLDKMTL+EVGPRFCLNPIKIFGGSFGGPTLYENP YVSPNQIRALEKKQKAGKY+KKVK
Sbjct: 241 GLDKMTLVEVGPRFCLNPIKIFGGSFGGPTLYENPFYVSPNQIRALEKKQKAGKYAKKVK 300

Query: 301 AKTRRKMHELSNPLEPDEFADMWKE 325
           AK RRKMHE+SNPLEPDEFADMW+E
Sbjct: 301 AKKRRKMHEMSNPLEPDEFADMWRE 313

[Close]
BLAST of Lsi06G001480.1 vs. TAIR10
Match: AT3G15460.1 (AT3G15460.1 Ribosomal RNA processing Brix domain protein)

HSP 1 Score: 531.9 bits (1369), Expect = 2.7e-151
Identity = 255/324 (78.70%), Postives = 281/324 (86.73%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLIT 60
           M +KRKHSE +    VK  DSAPERP+RTL+GWKDK+   E       +VFRNKEKVL+T
Sbjct: 1   MGRKRKHSETVTAAPVK--DSAPERPQRTLLGWKDKKEDAENSKPAYASVFRNKEKVLVT 60

Query: 61  CSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHK 120
           CSRRINFRYRHLMLN+VSLLPHCKKD+KVE++SS+GATLNEL+ELKG SS LFFECRKHK
Sbjct: 61  CSRRINFRYRHLMLNMVSLLPHCKKDSKVEAKSSRGATLNELIELKGSSSCLFFECRKHK 120

Query: 121 DLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEI 180
           DLY+WM K P GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+ D HWKLLKE+
Sbjct: 121 DLYMWMVKSPGGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFENDAHWKLLKEM 180

Query: 181 IVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGG 240
           + Q        IFG P+ HR SKPYHDHVFVFSIVDDHIWFRNYQI+VPHNESDK+ARG 
Sbjct: 181 LTQ--------IFGIPEGHRKSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKIARGD 240

Query: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA 300
           LDKMTLIEVGPRFCLNPIKIFGGSFGG TLYENP YVSPNQIRALEK+ KAGK++KK+KA
Sbjct: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGTTLYENPFYVSPNQIRALEKRNKAGKFAKKIKA 300

Query: 301 KTRRKMHELSNPLEPDEFADMWKE 325
           KTRRKMHELSNPLEPDEF DMW +
Sbjct: 301 KTRRKMHELSNPLEPDEFTDMWND 314

[Close]
BLAST of Lsi06G001480.1 vs. TAIR10
Match: AT1G52930.1 (AT1G52930.1 Ribosomal RNA processing Brix domain protein)

HSP 1 Score: 526.2 bits (1354), Expect = 1.5e-149
Identity = 253/326 (77.61%), Postives = 283/326 (86.81%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKK-DDSAPERPKRTLMGWKDK-EVAKECEPGCDHAVFRNKEKVL 60
           M +KRKHSE      VKK D+ AP+RPKRTL+GWKDK E   E       + F+NKEKVL
Sbjct: 1   MGRKRKHSETEAPAPVKKSDEPAPDRPKRTLLGWKDKSEGEAEKAKALTSSGFKNKEKVL 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRI+FRYR LMLNIVSLLPHCKKD+KVE++SSKGATLNEL+ELK  +S LFFECRK
Sbjct: 61  VTCSRRISFRYRSLMLNIVSLLPHCKKDSKVEAKSSKGATLNELIELKNSNSCLFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           HKDLY+WM K P+GPSVKFLV AVH MEE+KLTGNHLKGSRP+LTFSSNFDKD HWKLLK
Sbjct: 121 HKDLYMWMVKSPNGPSVKFLVKAVHAMEEMKLTGNHLKGSRPLLTFSSNFDKDAHWKLLK 180

Query: 181 EIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVAR 240
           E++ Q        +FG PKEHR SKPYHDHVFVFSIVD+HIWFRNYQI+VPHNESDK+A+
Sbjct: 181 EMLTQ--------VFGIPKEHRKSKPYHDHVFVFSIVDEHIWFRNYQISVPHNESDKIAK 240

Query: 241 GGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKV 300
           GGLDKMTLIEVGPRFCLNPIKIF GSFGGPTLYENPLYVSPNQIRALEK+ KAGK++KK+
Sbjct: 241 GGLDKMTLIEVGPRFCLNPIKIFAGSFGGPTLYENPLYVSPNQIRALEKRNKAGKFAKKI 300

Query: 301 KAKTRRKMHELSNPLEPDEFADMWKE 325
           KAKTR+KMHELSNPLEPDEFADMWK+
Sbjct: 301 KAKTRKKMHELSNPLEPDEFADMWKD 318

[Close]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match: gi|659099573|ref|XP_008450669.1| (PREDICTED: ribosome biogenesis protein BRX1-like [Cucumis melo])

HSP 1 Score: 622.9 bits (1605), Expect = 3.2e-175
Identity = 301/324 (92.90%), Postives = 310/324 (95.68%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLIT 60
           MAKKRKHSE +PTVSVKKD++A ERPKRTLMGWKDKEV K+ E G DHAVFRNKEKVL+T
Sbjct: 1   MAKKRKHSETVPTVSVKKDENALERPKRTLMGWKDKEVTKKSEAGSDHAVFRNKEKVLVT 60

Query: 61  CSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHK 120
           CSRRINFRYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHK
Sbjct: 61  CSRRINFRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHK 120

Query: 121 DLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEI 180
           DLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKE+
Sbjct: 121 DLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEM 180

Query: 181 IVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGG 240
           I+        QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQI+VPHNESDKVARGG
Sbjct: 181 II--------QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKVARGG 240

Query: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA 300
           LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA
Sbjct: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA 300

Query: 301 KTRRKMHELSNPLEPDEFADMWKE 325
           KTRRKMHELSNPLEPDEFADMWKE
Sbjct: 301 KTRRKMHELSNPLEPDEFADMWKE 316

[Close]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match: gi|778662534|ref|XP_011659901.1| (PREDICTED: LOW QUALITY PROTEIN: ribosome biogenesis protein BRX1-like [Cucumis sativus])

HSP 1 Score: 612.5 bits (1578), Expect = 4.4e-172
Identity = 300/324 (92.59%), Postives = 306/324 (94.44%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKEVAKECEPGCDHAVFRNKEKVLIT 60
           MAKKRKHSE LPTVSVKKDD APERPKRTLMGWKDK+V KE E G DHAVFRNKEKVLIT
Sbjct: 1   MAKKRKHSESLPTVSVKKDDDAPERPKRTLMGWKDKKVTKEIEVGSDHAVFRNKEKVLIT 60

Query: 61  CSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRKHK 120
           CSRRINFRY HLMLN+VSLLPHCKKDNKVE RSSKGATLNELVELKGCSSSLFFECRKHK
Sbjct: 61  CSRRINFRYWHLMLNMVSLLPHCKKDNKVELRSSKGATLNELVELKGCSSSLFFECRKHK 120

Query: 121 DLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLKEI 180
           DLYL MAKCPSGPSVKFLVNA+HTMEELKLTGNHLKGSRPILTFSSNFDKDVH KLLKE+
Sbjct: 121 DLYLCMAKCPSGPSVKFLVNAMHTMEELKLTGNHLKGSRPILTFSSNFDKDVHXKLLKEM 180

Query: 181 IVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVARGG 240
           I+        QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQI+VPHNESDKVARGG
Sbjct: 181 II--------QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKVARGG 240

Query: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA 300
           LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA
Sbjct: 241 LDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKVKA 300

Query: 301 KTRRKMHELSNPLEPDEFADMWKE 325
           KTRRKMHELSNPLEPDEFADMWKE
Sbjct: 301 KTRRKMHELSNPLEPDEFADMWKE 316

[Close]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match: gi|763778551|gb|KJB45674.1| (hypothetical protein B456_007G319600 [Gossypium raimondii])

HSP 1 Score: 566.2 bits (1458), Expect = 3.6e-158
Identity = 271/330 (82.12%), Postives = 298/330 (90.30%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECEPGCDHA-VFRNKEKVL 60
           M KKRKHSE       KKD++APERPKR+L GWKDKE V ++ E   D   VFRNKEKV+
Sbjct: 1   MGKKRKHSETQTAEPKKKDEAAPERPKRSLFGWKDKEEVQQQTESNTDPPQVFRNKEKVM 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNEL+EL+GCSS+LFFECRK
Sbjct: 61  VTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELIELRGCSSALFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           H+DLYLWMA+CP+GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+KD HWKLLK
Sbjct: 121 HQDLYLWMARCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAHWKLLK 180

Query: 181 EIIVQRNNMFSP----QIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESD 240
           E+I+Q ++ F      QIFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESD
Sbjct: 181 EMIIQFSDTFFSIIFFQIFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESD 240

Query: 241 KVARGGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKY 300
           K+ RGGLDKMTL+EVGPRFCLNPIKIF GSFGGPTLYENP YVSPNQIRALEK+QKAGKY
Sbjct: 241 KMVRGGLDKMTLVEVGPRFCLNPIKIFAGSFGGPTLYENPFYVSPNQIRALEKRQKAGKY 300

Query: 301 SKKVKAKTRRKMHELSNPLEPDEFADMWKE 325
           +KKVKAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 AKKVKAKTRRKMHELSNPLEPDEFADMWRE 330

[Close]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match: gi|823198009|ref|XP_012434459.1| (PREDICTED: ribosome biogenesis protein BRX1 [Gossypium raimondii])

HSP 1 Score: 562.4 bits (1448), Expect = 5.2e-157
Identity = 269/326 (82.52%), Postives = 294/326 (90.18%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECEPGCDHA-VFRNKEKVL 60
           M KKRKHSE       KKD++APERPKR+L GWKDKE V ++ E   D   VFRNKEKV+
Sbjct: 1   MGKKRKHSETQTAEPKKKDEAAPERPKRSLFGWKDKEEVQQQTESNTDPPQVFRNKEKVM 60

Query: 61  ITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECRK 120
           +TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVESRSSKGATLNEL+EL+GCSS+LFFECRK
Sbjct: 61  VTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESRSSKGATLNELIELRGCSSALFFECRK 120

Query: 121 HKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLLK 180
           H+DLYLWMA+CP+GPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFSSNF+KD HWKLLK
Sbjct: 121 HQDLYLWMARCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFEKDAHWKLLK 180

Query: 181 EIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVAR 240
           E+I+Q        IFGTPKEHR SKPYHDHVFVFSI DDHIWFRNYQI+VPHNESDK+ R
Sbjct: 181 EMIIQ--------IFGTPKEHRKSKPYHDHVFVFSIADDHIWFRNYQISVPHNESDKMVR 240

Query: 241 GGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKKV 300
           GGLDKMTL+EVGPRFCLNPIKIF GSFGGPTLYENP YVSPNQIRALEK+QKAGKY+KKV
Sbjct: 241 GGLDKMTLVEVGPRFCLNPIKIFAGSFGGPTLYENPFYVSPNQIRALEKRQKAGKYAKKV 300

Query: 301 KAKTRRKMHELSNPLEPDEFADMWKE 325
           KAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 KAKTRRKMHELSNPLEPDEFADMWRE 318

[Close]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match: gi|590608740|ref|XP_007021346.1| (Ribosome biogenesis protein brix, putative [Theobroma cacao])

HSP 1 Score: 560.8 bits (1444), Expect = 1.5e-156
Identity = 271/327 (82.87%), Postives = 291/327 (88.99%), Query Frame = 1 

Query: 1   MAKKRKHSEILPTVSVKKDDSAPERPKRTLMGWKDKE-VAKECE--PGCDHAVFRNKEKV 60
           M KKRK SE        KD+ APERPKRTL+GWKDKE VAK+ E  P     VFRNKEKV
Sbjct: 1   MGKKRKQSETQTAEPTNKDEGAPERPKRTLVGWKDKEEVAKQTESNPDSSSQVFRNKEKV 60

Query: 61  LITCSRRINFRYRHLMLNIVSLLPHCKKDNKVESRSSKGATLNELVELKGCSSSLFFECR 120
           ++TCSRRIN+RYRHLMLN+VSLLPHCKKDNKVES+SSKGATLNEL+EL GCSS LFFECR
Sbjct: 61  MVTCSRRINYRYRHLMLNMVSLLPHCKKDNKVESKSSKGATLNELIELSGCSSCLFFECR 120

Query: 121 KHKDLYLWMAKCPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPILTFSSNFDKDVHWKLL 180
           KH+DLYLWMAK PSGPSVKFLVNAVHTMEELKLTGNHLKGSRP+LTFS NF+KD HWKLL
Sbjct: 121 KHQDLYLWMAKSPSGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSGNFEKDAHWKLL 180

Query: 181 KEIIVQRNNMFSPQIFGTPKEHRHSKPYHDHVFVFSIVDDHIWFRNYQIAVPHNESDKVA 240
           KE+I+Q        IFGTPKEHR SKPYHDHVFVFSIVDDHIWFRNYQI+VPHNESDK+ 
Sbjct: 181 KEMIMQ--------IFGTPKEHRKSKPYHDHVFVFSIVDDHIWFRNYQISVPHNESDKMV 240

Query: 241 RGGLDKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPLYVSPNQIRALEKKQKAGKYSKK 300
           RGGLDKMTL+EVGPRFCLNPIKIFGGSFGGPTLYENP YVSPNQIRALEK+QKAGKY+KK
Sbjct: 241 RGGLDKMTLVEVGPRFCLNPIKIFGGSFGGPTLYENPFYVSPNQIRALEKRQKAGKYAKK 300

Query: 301 VKAKTRRKMHELSNPLEPDEFADMWKE 325
           VKAKTRRKMHELSNPLEPDEFADMW+E
Sbjct: 301 VKAKTRRKMHELSNPLEPDEFADMWRE 319

The following BLAST results are available for this feature:
BLAST of Lsi06G001480.1 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BRX1_XENLA1.0e-6443.65Ribosome biogenesis protein BRX1 homolog OS=Xenopus laevis GN=brix1 PE=2 SV=1[more]
BRX1_HUMAN1.4e-6443.96Ribosome biogenesis protein BRX1 homolog OS=Homo sapiens GN=BRIX1 PE=1 SV=2[more]
BRX1_RAT2.3e-6445.97Ribosome biogenesis protein BRX1 homolog OS=Rattus norvegicus GN=Brix1 PE=2 SV=1[more]
BRX1_PONAB5.2e-6443.65Ribosome biogenesis protein BRX1 homolog OS=Pongo abelii GN=BRIX1 PE=2 SV=1[more]
BRX1_MOUSE5.2e-6445.67Ribosome biogenesis protein BRX1 homolog OS=Mus musculus GN=Brix1 PE=1 SV=3[more]
BLAST of Lsi06G001480.1 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0D2SNA9_GOSRA2.5e-15882.12Uncharacterized protein OS=Gossypium raimondii GN=B456_007G319600 PE=4 SV=1[more]
A0A0D2PI01_GOSRA3.6e-15782.52Uncharacterized protein OS=Gossypium raimondii GN=B456_007G319600 PE=4 SV=1[more]
A0A0B0NEE6_GOSAR3.6e-15782.52Ribosome biogenesis BRX1 OS=Gossypium arboreum GN=F383_18642 PE=4 SV=1[more]
A0A061F737_THECC1.1e-15682.87Ribosome biogenesis protein brix, putative OS=Theobroma cacao GN=TCM_031378 PE=4... [more]
A0A067JSR7_JATCU5.2e-15684.00Uncharacterized protein OS=Jatropha curcas GN=JCGZ_22878 PE=4 SV=1[more]
BLAST of Lsi06G001480.1 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G15460.12.7e-15178.70 Ribosomal RNA processing Brix domain protein[more]
AT1G52930.11.5e-14977.61 Ribosomal RNA processing Brix domain protein[more]
BLAST of Lsi06G001480.1 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659099573|ref|XP_008450669.1|3.2e-17592.90PREDICTED: ribosome biogenesis protein BRX1-like [Cucumis melo][more]
gi|778662534|ref|XP_011659901.1|4.4e-17292.59PREDICTED: LOW QUALITY PROTEIN: ribosome biogenesis protein BRX1-like [Cucumis s... [more]
gi|763778551|gb|KJB45674.1|3.6e-15882.12hypothetical protein B456_007G319600 [Gossypium raimondii][more]
gi|823198009|ref|XP_012434459.1|5.2e-15782.52PREDICTED: ribosome biogenesis protein BRX1 [Gossypium raimondii][more]
gi|590608740|ref|XP_007021346.1|1.5e-15682.87Ribosome biogenesis protein brix, putative [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR026532BRX1
IPR007109Brix
IPR004154Anticodon-bd
GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi06G001480Lsi06G001480gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi06G001480.1Lsi06G001480.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G001480.1.five_prime_UTR.1Lsi06G001480.1.five_prime_UTR.1five_prime_UTR
Lsi06G001480.1.five_prime_UTR.2Lsi06G001480.1.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G001480.1.CDS.1Lsi06G001480.1.CDS.1CDS
Lsi06G001480.1.CDS.2Lsi06G001480.1.CDS.2CDS
Lsi06G001480.1.CDS.3Lsi06G001480.1.CDS.3CDS
Lsi06G001480.1.CDS.4Lsi06G001480.1.CDS.4CDS
Lsi06G001480.1.CDS.5Lsi06G001480.1.CDS.5CDS
Lsi06G001480.1.CDS.6Lsi06G001480.1.CDS.6CDS
Lsi06G001480.1.CDS.7Lsi06G001480.1.CDS.7CDS
Lsi06G001480.1.CDS.8Lsi06G001480.1.CDS.8CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi06G001480.1.three_prime_UTR.1Lsi06G001480.1.three_prime_UTR.1three_prime_UTR


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004154Anticodon-bindingunknownSSF52954Class II aaRS ABD-relatedcoord: 56..263
score: 6.87
IPR007109Brix domainPFAMPF04427Brixcoord: 62..259
score: 8.9
IPR007109Brix domainSMARTSM00879Brix_2coord: 58..260
score: 5.9
IPR007109Brix domainPROFILEPS50833BRIXcoord: 55..266
score: 29
IPR026532Ribosome biogenesis protein BRX1PANTHERPTHR13634RIBOSOME BIOGENESIS PROTEIN BRIXcoord: 2..324
score: 5.0E