Lsi05G012890.1 (mRNA) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi05G012890.1 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 553/760 (72.76%), Postives = 655/760 (86.18%), Query Frame = 1

Query: 12  LLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEE 71
           L+F      +     KTYV+ MDKSAMP  +T+HL WYS+ +++V  +  +E  G     
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN-- 79

Query: 72  RIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNS 131
           RI+Y+Y   FHG+AA+L+++EAE+LEEEDGV+A+ PET+YELHTTRSP FLGLE  +S  
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 132 AWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVG 191
            W++++ DHDV+VGVLDTGIWPES+SFND GMSPVPA W+G CETG+ F K+NCNRKIVG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 192 ARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMA 251
           ARVFY GY+AATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 252 PGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 311
             AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAM
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 312 EMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGR 371
           EMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 372 ITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 431
             +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 432 AGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRLG 491
           AGG+GM+L+NTA NGEELVAD H++PAVAVGE+EGK IKQYA+T++KATA+L  LGTR+G
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 492 VKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 551
           +KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILS
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 552 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 611
           GTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDH
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 612 GAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC-HRLLPNPGDL 671
           GAGHI+P +A DPGLVY+I PQ+YF+FLCTQDL+P+QLKVF+K+SNRTC H L  NPG+L
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNL 679

Query: 672 NYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYE 731
           NYPAISA+FPE T V ++TL RTVTNVGP  SSY   V+PF GA+V V+P++LNFT +++
Sbjct: 680 NYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQ 739

Query: 732 KLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           KLSY +TF T+ R   PEFGGL+WK  THKVRSP++ITWL
Sbjct: 740 KLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Lsi05G012890.1 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 2.0e-231
Identity = 410/762 (53.81%), Postives = 529/762 (69.42%), Query Frame = 1

Query: 11  LLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGE 70
           LL    C + S++    TY+V M KS MP SF  H +WY + L ++  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  ERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSN 130
             ++Y+Y N  HG + RL+++EA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++
Sbjct: 74  --LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD 133

Query: 131 SAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIV 190
             + +  +  DV+VGVLDTG+WPES S++D G  P+P+ WKG CE G  FT   CNRK++
Sbjct: 134 -LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 193

Query: 191 GARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGM 250
           GAR F  GY++  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA GTARGM
Sbjct: 194 GARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGM 253

Query: 251 APGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGA 310
           AP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF A
Sbjct: 254 APRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 313

Query: 311 MEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRG 370
           ME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G
Sbjct: 314 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 373

Query: 371 RITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVK 430
              +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK
Sbjct: 374 E-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 433

Query: 431 NAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRL 490
            AGGVGMIL+NTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+   TA++  LGT +
Sbjct: 434 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVV 493

Query: 491 GVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNIL 550
           GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+
Sbjct: 494 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 553

Query: 551 SGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYD 610
           SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+D
Sbjct: 554 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 613

Query: 611 HGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHRLLP-NPGD 670
           HGAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+  N TC      +  D
Sbjct: 614 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVAD 673

Query: 671 LNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRY 730
           LNYP+ +        V +    RTVT+VG A +    V +   G  + VEP  LNF    
Sbjct: 674 LNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEAN 733

Query: 731 EKLSYRITFL--TKKRQSMPEFGGLIWKDGTHKVRSPIVITW 770
           EK SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 EKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi05G012890.1 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 6.0e-220
Identity = 394/760 (51.84%), Postives = 513/760 (67.50%), Query Frame = 1

Query: 18  FLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEERIIYSY 77
           FLL      KTY+++++ S  P+SF  H  WY++ L++              E  ++Y+Y
Sbjct: 19  FLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTY 78

Query: 78  HNVFHGIAARLSEQEAEKL-EEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQ 137
              FHG +A L   EA+ L    + +L IF +  Y LHTTR+P+FLGL            
Sbjct: 79  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLG 138

Query: 138 IADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY 197
            + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G  F  + CN+K++GAR F 
Sbjct: 139 SSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFS 198

Query: 198 HGYQAATGK-FNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGAR 257
            G+Q A+G  F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR
Sbjct: 199 KGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRAR 258

Query: 258 IAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGV 317
           +A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GV
Sbjct: 259 VATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGV 318

Query: 318 FVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIP 377
           FVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G     
Sbjct: 319 FVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGM 378

Query: 378 ENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGV 437
             K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+
Sbjct: 379 GTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 438

Query: 438 GMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRLGVKPS 497
           GMI++NTAA+GEELVAD HL+PA+AVG++ G  +++Y  ++ K TA L F GT L VKPS
Sbjct: 439 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 498

Query: 498 PVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSM 557
           PVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSM
Sbjct: 499 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 558

Query: 558 SCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGH 617
           SCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH
Sbjct: 559 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 618

Query: 618 INPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHRLLPNPGDLNYPAI 677
           ++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  +  C +   +PG LNYP+ 
Sbjct: 619 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 678

Query: 678 SAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYEKLSYR 737
           S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F    EK  Y 
Sbjct: 679 SVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 738

Query: 738 ITFLTKKRQSM---PEFGGLIWKDGTHKVRSPIVITWLSF 773
           +TF++KK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 739 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Lsi05G012890.1 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 6.9e-216
Identity = 381/755 (50.46%), Postives = 514/755 (68.08%), Query Frame = 1

Query: 28  TYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEERIIYSYHNVFHGIAAR 87
           TY+V +D  A P  F  H HWY++ L+++                II++Y  VFHG +AR
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSAR 86

Query: 88  LSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVL 147
           L+ Q+A +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+
Sbjct: 87  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVI 146

Query: 148 DTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYHGYQAATGKFN 207
           DTG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK N
Sbjct: 147 DTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMN 206

Query: 208 EQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGC 267
           E  E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC
Sbjct: 207 ETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGC 266

Query: 268 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 327
           + SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP 
Sbjct: 267 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 328 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGS 387
            +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGS 386

Query: 388 NSSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILSNTAANG 447
                   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +G
Sbjct: 387 LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDG 446

Query: 448 EELVADCHLVPAVAVGEREGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAA 507
           E LVADCH++PA +VG   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+
Sbjct: 447 EGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVAS 506

Query: 508 FSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHV 567
           FS+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHV
Sbjct: 507 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 566

Query: 568 SGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRK 627
           SG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P K
Sbjct: 567 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 626

Query: 628 ALDPGLVYEIQPQDYFDFLCTQDLTPTQL-KVFSKYSNRTCHRLLPNPGDLNYPAISAVF 687
           A+DPGLVY+I   DY +FLC  + T T +  +  + ++    R   + G+LNYP+ S VF
Sbjct: 627 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVF 686

Query: 688 PE-KTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYEKLSYRITF 747
            +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  +KLS+ +  
Sbjct: 687 QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRV 746

Query: 748 LTKKRQSMP-----EFGGLIWKDGTHKVRSPIVIT 769
            T + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 KTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi05G012890.1 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 9.0e-216
Identity = 396/773 (51.23%), Postives = 516/773 (66.75%), Query Frame = 1

Query: 9   LCLLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGG 68
           L L L F     + +Q  KT++ ++D  +MP  F  H HWYST  +              
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  GEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPAD 128
            E RI++ YH VFHG +A ++  EA+ L     VLA+F + + ELHTTRSP+FLGL+  +
Sbjct: 68  -ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--N 127

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRK 188
               WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRK
Sbjct: 128 QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRK 187

Query: 189 IVGARVFYHGYQAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTA 248
           I+GAR F  G QAA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G A
Sbjct: 188 IIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVA 247

Query: 249 RGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSL 308
           +G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D +
Sbjct: 248 KGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPI 307

Query: 309 SVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVT 368
           ++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  + 
Sbjct: 308 AIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLR 367

Query: 369 GVSLYRGRITIPEN-KQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPR 428
           GVSLY G   +P N + FPVVY G +  S   +SLC+E TLDP  V GKIVICDRG SPR
Sbjct: 368 GVSLYAG---VPLNGRMFPVVYPGKSGMSS--ASLCMENTLDPKQVRGKIVICDRGSSPR 427

Query: 429 VQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATAT 488
           V KG+VVK AGGVGMIL+N A+NGE LV D HL+PA AVG  EG  IK YA ++    A+
Sbjct: 428 VAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIAS 487

Query: 489 LGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDT 548
           + F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D 
Sbjct: 488 IDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDP 547

Query: 549 RRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSG 608
           R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S 
Sbjct: 548 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDEST 607

Query: 609 ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHR 668
              ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R    
Sbjct: 608 GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTT 667

Query: 669 LLPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 728
             P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P 
Sbjct: 668 RKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPP 727

Query: 729 SLNFTRRYEKLSYRITFLTKKR-----QSMPEFGGLIWKD-GTHKVRSPIVIT 769
            L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 RLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Lsi05G012890.1 vs. TrEMBL
Match: A0A0A0LB64_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G178520 PE=4 SV=1)

HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 727/773 (94.05%), Postives = 754/773 (97.54%), Query Frame = 1

Query: 1   MADTPLKWLCLLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNL 60
           MAD+PLK LC LLF   FLLS+A F KTYVVQMD+SAMPDSFT+H  WYS VL+NVV++L
Sbjct: 35  MADSPLKCLCFLLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDL 94

Query: 61  EREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPK 120
           +REG+GGGGEERIIY YHNVFHG+AARLSE+E EKLEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 95  QREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 154

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESDSF+DAGMSPVPAHWKGECETGRGF
Sbjct: 155 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 214

Query: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFY GYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 215 TKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 274

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 275 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 334

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 335 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 394

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T+TGVSLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS
Sbjct: 395 TITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 454

Query: 421 PRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGG+GMILSNTAANGEELVADCHLVPAVA+GEREGKAIKQYALTNR+AT
Sbjct: 455 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRAT 514

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 515 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 574

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS
Sbjct: 575 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 634

Query: 601 SGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660
           S ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDL+PTQLKVFSKYSNRTC
Sbjct: 635 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTC 694

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEP 720
             LLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPATSSYHAVV+PF GA VKVEP
Sbjct: 695 RGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEP 754

Query: 721 ESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWLSFV 774
           ESLNFTRRYEK+SYRITF+TKKRQSMPEFGGLIWKDG+HKVRSPIVITWLSFV
Sbjct: 755 ESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 807

BLAST of Lsi05G012890.1 vs. TrEMBL
Match: M5XPU8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa1027166mg PE=4 SV=1)

HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 610/777 (78.51%), Postives = 690/777 (88.80%), Query Frame = 1

Query: 1   MADT-PLKWLCLLL----FFHCFLLSTAQF-FKTYVVQMDKSAMPDSFTDHLHWYSTVLS 60
           MAD  P+KWL L+L    FF     +  QF  KTY+VQMDKSA P+SFT+HL WYS+ ++
Sbjct: 1   MADQKPVKWLVLILTNCLFFSIAFSAKTQFAHKTYIVQMDKSAKPESFTNHLDWYSSKVN 60

Query: 61  NVVVNLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELH 120
           ++V   E E  GG  +ER+IY+Y N FHG+AARLSE+EAE+L+E+DGVLAIFP+TKY+LH
Sbjct: 61  SIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEEAERLQEQDGVLAIFPDTKYQLH 120

Query: 121 TTRSPKFLGLEPADSNSA-WSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGE 180
           TTRSP FLGLEP DS +  WSQ++ DHDVIVGVLDTG+WPES SFND GMSPVPA+WKG 
Sbjct: 121 TTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGVWPESQSFNDTGMSPVPAYWKGA 180

Query: 181 CETGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP 240
           CETGRGF+K NCN+KIVGAR+FYHGY+AATGK NEQ E+KSPRDQDGHGTHTAATVAGSP
Sbjct: 181 CETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTEFKSPRDQDGHGTHTAATVAGSP 240

Query: 241 VAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGG 300
           V GA+LLGYA+GTARGMAPGARIAAYKVCWVGGCFSSDILSAVD+AVADGVNVLSISLGG
Sbjct: 241 VRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDKAVADGVNVLSISLGG 300

Query: 301 GVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 360
           GVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP+ 
Sbjct: 301 GVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPSS 360

Query: 361 VKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 420
           VKLG+GRTVTGVSLY+GR+ +  NKQ+PVVYMG NS+SPDPSSLCLEGTLD   VAGKIV
Sbjct: 361 VKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTSPDPSSLCLEGTLDRRVVAGKIV 420

Query: 421 ICDRGISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYA 480
           ICDRGISPRVQKG VVK+AGGVGMIL+NTAANGEELVADCHLVPAVAVGE E KAIK YA
Sbjct: 421 ICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVADCHLVPAVAVGETEAKAIKHYA 480

Query: 481 LTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 540
           LT+ +ATATL FLGTR GV+PSPVVAAFSSRGPNF++LEILKPD+VAPGVNILAAWTG  
Sbjct: 481 LTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSLEILKPDVVAPGVNILAAWTGAL 540

Query: 541 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 600
           GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT
Sbjct: 541 GPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNT 600

Query: 601 YKPLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFS 660
           +KPL+D+S A  S+PYDHGAGHINPRKALDPGLVY+I+ QDY +FLCTQ LTP QLKVF+
Sbjct: 601 HKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIEAQDYLEFLCTQRLTPMQLKVFT 660

Query: 661 KYSNRTCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNG 720
           KYSNR+C   L +PGDLNYPAIS VFPE+T+V+ LTLHRTVTNVGP  S+YHA+V+PF G
Sbjct: 661 KYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLHRTVTNVGPPVSNYHAIVSPFKG 720

Query: 721 AAVKVEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           A VKVEP +L FTR  +KLSY+ITF TK RQ+ PEFGGL+WKDG H+VRSPIV+ WL
Sbjct: 721 AYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGGLVWKDGVHRVRSPIVVVWL 777

BLAST of Lsi05G012890.1 vs. TrEMBL
Match: A0A061G0C3_THECC (Subtilase 1.3 OS=Theobroma cacao GN=TCM_015025 PE=4 SV=1)

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 606/777 (77.99%), Postives = 681/777 (87.64%), Query Frame = 1

Query: 1   MADTPLKWLCLLL-----FFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSN 60
           M + P+KWL L+L     F      S     KT++VQMDKSAMP SF+ HL WYS+ + +
Sbjct: 1   MTENPVKWLFLILTSCLSFALVLSASNPLIKKTFIVQMDKSAMPASFSSHLEWYSSKVKS 60

Query: 61  VVV-NLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELH 120
           V++ N + EG G G  ERIIYSY N FHG+AA+L+E EAE+LEEEDGV+AI PE KY+LH
Sbjct: 61  VIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTEDEAERLEEEDGVVAILPEMKYQLH 120

Query: 121 TTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGEC 180
           TTRSP FLGLEP +S S WSQ++ DHDVIVGVLDTGIWPES+SFND G++PVPAHWKG C
Sbjct: 121 TTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGIWPESESFNDTGLAPVPAHWKGAC 180

Query: 181 ETGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPV 240
           ETGRGF K +CNRKIVGARVFY GY+AATGK NE+ EYKSPRDQDGHGTHTAATVAGSPV
Sbjct: 181 ETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAGSPV 240

Query: 241 AGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGG 300
            GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGV+VLSISLGGG
Sbjct: 241 RGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVADGVSVLSISLGGG 300

Query: 301 VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIV 360
           VSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPA V
Sbjct: 301 VSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPADV 360

Query: 361 KLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVI 420
           KLG GRT+TGVSLY+G+  +  NKQ+P+VYMGSNSSSPDPSSLCLEGTLDPH V+GKIVI
Sbjct: 361 KLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSSPDPSSLCLEGTLDPHIVSGKIVI 420

Query: 421 CDRGISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYAL 480
           CDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVADCHL+PA+AVGE EGKAIK YAL
Sbjct: 421 CDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVADCHLLPALAVGEMEGKAIKHYAL 480

Query: 481 TNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTG 540
           T+RKATATL FLGTRLG++PSPVVAAFSSRGPNFLT EILKPD+VAPGVNILAAWTG+ G
Sbjct: 481 TSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTFEILKPDMVAPGVNILAAWTGELG 540

Query: 541 PSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTY 600
           PSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT+
Sbjct: 541 PSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNTH 600

Query: 601 KPLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSK 660
            PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+ QDYF+FLCTQ LT  QLKVF K
Sbjct: 601 NPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIEAQDYFEFLCTQKLTTMQLKVFGK 660

Query: 661 YSNRTCHRLLPNPGDLNYPAISAVFPEKTSVTS-LTLHRTVTNVGPATSSYHAVVTPFNG 720
           YSNR CH  L + GDLNYPAIS VFPE T+  S LTLHRTVTNVGP  S+YH VV+ F G
Sbjct: 661 YSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTLHRTVTNVGPPISNYHVVVSQFKG 720

Query: 721 AAVKVEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           A VKV+P+SLNFTR+ +KLSY+ITF TK  Q++PEFGGL+WKDG HKVRSPIVITW+
Sbjct: 721 ATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFGGLVWKDGVHKVRSPIVITWI 775

BLAST of Lsi05G012890.1 vs. TrEMBL
Match: W9SBY0_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_012947 PE=4 SV=1)

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 595/773 (76.97%), Postives = 676/773 (87.45%), Query Frame = 1

Query: 1   MADTPLKWLCLLLFFHCFLLSTAQFF--KTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVV 60
           M  TP+KWL L+    C L +  +    KTY++QMDKSAMP+SF+DHL WYS+ + +V++
Sbjct: 1   MVGTPVKWLVLISLTSCLLFNAVELSAKKTYIIQMDKSAMPESFSDHLEWYSSKVKSVLM 60

Query: 61  NLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRS 120
               +      EERIIYSY   FHG+AA+LSE+EAEKLE++D VL +FPETKYELHTTRS
Sbjct: 61  KQSDD------EERIIYSYQTAFHGVAAQLSEEEAEKLEQDDDVLGVFPETKYELHTTRS 120

Query: 121 PKFLGLEPA-DSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETG 180
           P FLGL+P  D ++ WSQ ++DHDV+VGVLDTGIWPES+SFND GM+PVPAHWKGECETG
Sbjct: 121 PMFLGLDPRQDRDNVWSQMLSDHDVVVGVLDTGIWPESESFNDTGMTPVPAHWKGECETG 180

Query: 181 RGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGA 240
           RGF K++CNRKIVGAR+FY GY+AATGK N Q EYKSPRDQDGHGTHTAATVAGSPV GA
Sbjct: 181 RGFAKRHCNRKIVGARMFYRGYEAATGKINPQSEYKSPRDQDGHGTHTAATVAGSPVKGA 240

Query: 241 SLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSS 300
           +LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSS
Sbjct: 241 NLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSS 300

Query: 301 YYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG 360
           YYRDSLSVA+FGAMEMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VKLG
Sbjct: 301 YYRDSLSVASFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKLG 360

Query: 361 DGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDR 420
            G+T++GVSLY+GR T+  NKQ+P++YMGSNS+SPDPSSLCLEGTLD   VAGKIVICDR
Sbjct: 361 TGKTISGVSLYKGRKTLGSNKQYPIIYMGSNSTSPDPSSLCLEGTLDRRKVAGKIVICDR 420

Query: 421 GISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNR 480
           GISPRVQKG VVK+AGGVGMIL+NTAANGEELVAD HL+PAVAVGE + K IK YALTN 
Sbjct: 421 GISPRVQKGQVVKDAGGVGMILANTAANGEELVADSHLIPAVAVGESKAKEIKHYALTNP 480

Query: 481 KATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSS 540
           K TA+L FLGTRLG++PSPVVAAFSSRGPNFLTLEILKPD+VAPGVNILAAWTG  GPSS
Sbjct: 481 KTTASLAFLGTRLGIRPSPVVAAFSSRGPNFLTLEILKPDMVAPGVNILAAWTGDLGPSS 540

Query: 541 LTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL 600
           L TD RRVKFNILSGTSMSCPHVSG+AAL+K++HPDWSP+AIKSALMTTAYVHDNT  PL
Sbjct: 541 LPTDKRRVKFNILSGTSMSCPHVSGIAALLKARHPDWSPAAIKSALMTTAYVHDNTLNPL 600

Query: 601 KDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSN 660
           KD+S A PS+PYDHGAGH+NPRKALDPGLVY+I+PQDY++FLCTQ LTPTQLKVFSKY+N
Sbjct: 601 KDASTAVPSNPYDHGAGHVNPRKALDPGLVYDIRPQDYYEFLCTQSLTPTQLKVFSKYAN 660

Query: 661 RTCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVK 720
           RTC   L  PG+LNYPAIS VFPEK SV+ +++ RTVTNVGP  S+YH  V+PF GA+VK
Sbjct: 661 RTCKHSLAGPGELNYPAISVVFPEKASVSVISVRRTVTNVGPPVSNYHVSVSPFRGASVK 720

Query: 721 VEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           VEP+SL+F +  +KLSY++TF TK  Q+ PEFG L+WKDG HKVRSPI IT L
Sbjct: 721 VEPKSLSFNKAGQKLSYKVTFTTKSLQTAPEFGDLVWKDGVHKVRSPIAITRL 767

BLAST of Lsi05G012890.1 vs. TrEMBL
Match: A0A0D2ST22_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G065800 PE=4 SV=1)

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 602/776 (77.58%), Postives = 674/776 (86.86%), Query Frame = 1

Query: 1   MADTPLKWLCLLL---FFHCFLLSTAQFF--KTYVVQMDKSAMPDSFTDHLHWYSTVLSN 60
           MA+ P+KWL L+L       F+LS +     KTY+VQM KSAMP SF+  L WYS+ L +
Sbjct: 1   MAENPVKWLFLILASCLCFAFVLSESNLLIKKTYIVQMHKSAMPASFSSPLEWYSSKLKS 60

Query: 61  VVVNLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHT 120
           V+ + + EG G G E RIIYSY N FHG+AA+L+E+EAE+L++EDGV+AI PETKYELHT
Sbjct: 61  VMSDTQSEGEGDG-ENRIIYSYQNAFHGVAAQLTEEEAERLKQEDGVVAILPETKYELHT 120

Query: 121 TRSPKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECE 180
           TRSP FLGLEP +S S WSQ++ADHDVIVGVLDTGIWPES SFND GM+PVPAHWKG CE
Sbjct: 121 TRSPMFLGLEPEESTSIWSQKLADHDVIVGVLDTGIWPESASFNDTGMTPVPAHWKGTCE 180

Query: 181 TGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVA 240
           TGRGF K +CNRKIVGARVFY GY+AATGK NE+ EYKSPRDQDGHGTHTAATVAGSPV 
Sbjct: 181 TGRGFQKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAGSPVR 240

Query: 241 GASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGV 300
           GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAV DGVNVLSISLGGG 
Sbjct: 241 GANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVGDGVNVLSISLGGGA 300

Query: 301 SSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVK 360
           SSY  DSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP  VK
Sbjct: 301 SSYSHDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPGSVK 360

Query: 361 LGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVIC 420
           LG GRT++GVSLY+GR  +  NKQ+P+VYMGSNSSSP+PSSLCLEGTLDPH V+GKIVIC
Sbjct: 361 LGSGRTISGVSLYKGRRLLQANKQYPLVYMGSNSSSPNPSSLCLEGTLDPHVVSGKIVIC 420

Query: 421 DRGISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALT 480
           DRGI+PRVQKG VVK+AGGVGMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALT
Sbjct: 421 DRGINPRVQKGQVVKDAGGVGMILTNTAANGEELVADCHLLPAVAVGEMEGKAIKHYALT 480

Query: 481 NRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGP 540
           N K TATL FLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPD+VAPGVNILAAWTG+ GP
Sbjct: 481 NGKPTATLAFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDVVAPGVNILAAWTGELGP 540

Query: 541 SSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYK 600
           SSL TD RRV+FNILSGTSMSCPHVSG+AALIK++HPDWSP+A+KSALMTTAYVHDN + 
Sbjct: 541 SSLPTDHRRVRFNILSGTSMSCPHVSGIAALIKARHPDWSPAAVKSALMTTAYVHDNIHN 600

Query: 601 PLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKY 660
           PL+DSS A+ S+PYDHGAGHINP KALDPGL+Y+I  QDYF+FLCTQ LT  QLK FSK+
Sbjct: 601 PLQDSSTAAASTPYDHGAGHINPLKALDPGLIYDISAQDYFEFLCTQKLTAMQLKAFSKH 660

Query: 661 SNRTC-HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGA 720
           SN +C H  L  PGDLNYPAIS VFPE T++++LTLHRTVTNVGP  S YH VV+PF G 
Sbjct: 661 SNMSCHHNTLATPGDLNYPAISVVFPEDTAISTLTLHRTVTNVGPPASHYHVVVSPFKGV 720

Query: 721 AVKVEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
            +KVEP++LNFTRR +KLSY+I+F  K  Q+MPEFGGL WKDG HKVRSPI ITWL
Sbjct: 721 TIKVEPKTLNFTRRNQKLSYKISFTRKSPQTMPEFGGLAWKDGVHKVRSPIAITWL 775

BLAST of Lsi05G012890.1 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 553/760 (72.76%), Postives = 655/760 (86.18%), Query Frame = 1

Query: 12  LLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEE 71
           L+F      +     KTYV+ MDKSAMP  +T+HL WYS+ +++V  +  +E  G     
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNN-- 79

Query: 72  RIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNS 131
           RI+Y+Y   FHG+AA+L+++EAE+LEEEDGV+A+ PET+YELHTTRSP FLGLE  +S  
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 132 AWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVG 191
            W++++ DHDV+VGVLDTGIWPES+SFND GMSPVPA W+G CETG+ F K+NCNRKIVG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 192 ARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMA 251
           ARVFY GY+AATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 252 PGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 311
             AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAM
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 312 EMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGR 371
           EMGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 372 ITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 431
             +P+NKQ+P+VY+G N+SSPDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 432 AGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRLG 491
           AGG+GM+L+NTA NGEELVAD H++PAVAVGE+EGK IKQYA+T++KATA+L  LGTR+G
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIG 499

Query: 492 VKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 551
           +KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILS
Sbjct: 500 IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILS 559

Query: 552 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 611
           GTSMSCPHVSGVAALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDH
Sbjct: 560 GTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDH 619

Query: 612 GAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC-HRLLPNPGDL 671
           GAGHI+P +A DPGLVY+I PQ+YF+FLCTQDL+P+QLKVF+K+SNRTC H L  NPG+L
Sbjct: 620 GAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNL 679

Query: 672 NYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYE 731
           NYPAISA+FPE T V ++TL RTVTNVGP  SSY   V+PF GA+V V+P++LNFT +++
Sbjct: 680 NYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQ 739

Query: 732 KLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           KLSY +TF T+ R   PEFGGL+WK  THKVRSP++ITWL
Sbjct: 740 KLSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Lsi05G012890.1 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 410/762 (53.81%), Postives = 529/762 (69.42%), Query Frame = 1

Query: 11  LLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGE 70
           LL    C + S++    TY+V M KS MP SF  H +WY + L ++  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  ERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSN 130
             ++Y+Y N  HG + RL+++EA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++
Sbjct: 74  --LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD 133

Query: 131 SAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIV 190
             + +  +  DV+VGVLDTG+WPES S++D G  P+P+ WKG CE G  FT   CNRK++
Sbjct: 134 -LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLI 193

Query: 191 GARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGM 250
           GAR F  GY++  G  +E  E +SPRD DGHGTHT++T AGS V GASLLGYA GTARGM
Sbjct: 194 GARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGM 253

Query: 251 APGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGA 310
           AP AR+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF A
Sbjct: 254 APRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAA 313

Query: 311 MEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRG 370
           ME G+ VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G
Sbjct: 314 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 373

Query: 371 RITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVK 430
              +P+ K  P +Y G N+S+    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK
Sbjct: 374 E-ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 433

Query: 431 NAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRL 490
            AGGVGMIL+NTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+   TA++  LGT +
Sbjct: 434 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVV 493

Query: 491 GVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNIL 550
           GVKPSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+
Sbjct: 494 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 553

Query: 551 SGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYD 610
           SGTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+D
Sbjct: 554 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 613

Query: 611 HGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHRLLP-NPGD 670
           HGAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+  N TC      +  D
Sbjct: 614 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVAD 673

Query: 671 LNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRY 730
           LNYP+ +        V +    RTVT+VG A +    V +   G  + VEP  LNF    
Sbjct: 674 LNYPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEAN 733

Query: 731 EKLSYRITFL--TKKRQSMPEFGGLIWKDGTHKVRSPIVITW 770
           EK SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 EKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi05G012890.1 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 765.4 bits (1975), Expect = 3.4e-221
Identity = 394/760 (51.84%), Postives = 513/760 (67.50%), Query Frame = 1

Query: 18  FLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEERIIYSY 77
           FLL      KTY+++++ S  P+SF  H  WY++ L++              E  ++Y+Y
Sbjct: 19  FLLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS--------------ESSLLYTY 78

Query: 78  HNVFHGIAARLSEQEAEKL-EEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQ 137
              FHG +A L   EA+ L    + +L IF +  Y LHTTR+P+FLGL            
Sbjct: 79  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLG 138

Query: 138 IADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFY 197
            + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G  F  + CN+K++GAR F 
Sbjct: 139 SSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFS 198

Query: 198 HGYQAATGK-FNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGAR 257
            G+Q A+G  F+ + E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR
Sbjct: 199 KGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRAR 258

Query: 258 IAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGV 317
           +A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GV
Sbjct: 259 VATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGV 318

Query: 318 FVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIP 377
           FVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G     
Sbjct: 319 FVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGM 378

Query: 378 ENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGV 437
             K   +VY   NSSS   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+
Sbjct: 379 GTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 438

Query: 438 GMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATATLGFLGTRLGVKPS 497
           GMI++NTAA+GEELVAD HL+PA+AVG++ G  +++Y  ++ K TA L F GT L VKPS
Sbjct: 439 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 498

Query: 498 PVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSM 557
           PVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSM
Sbjct: 499 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 558

Query: 558 SCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGH 617
           SCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+PY HG+GH
Sbjct: 559 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 618

Query: 618 INPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHRLLPNPGDLNYPAI 677
           ++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  +  C +   +PG LNYP+ 
Sbjct: 619 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 678

Query: 678 SAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYEKLSYR 737
           S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F    EK  Y 
Sbjct: 679 SVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 738

Query: 738 ITFLTKKRQSM---PEFGGLIWKDGTHKVRSPIVITWLSF 773
           +TF++KK  SM    EFG + W +  H+VRSP+  +W  F
Sbjct: 739 VTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Lsi05G012890.1 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 751.9 bits (1940), Expect = 3.9e-217
Identity = 381/755 (50.46%), Postives = 514/755 (68.08%), Query Frame = 1

Query: 28  TYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGGGEERIIYSYHNVFHGIAAR 87
           TY+V +D  A P  F  H HWY++ L+++                II++Y  VFHG +AR
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLT----------SSPPSIIHTYDTVFHGFSAR 86

Query: 88  LSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVIVGVL 147
           L+ Q+A +L +   V+++ PE    LHTTRSP+FLGL   D      +     D+++GV+
Sbjct: 87  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVI 146

Query: 148 DTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRKIVGARVFYHGYQAATGKFN 207
           DTG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GY+A  GK N
Sbjct: 147 DTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMN 206

Query: 208 EQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGC 267
           E  E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC
Sbjct: 207 ETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGC 266

Query: 268 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPD 327
           + SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP 
Sbjct: 267 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 326

Query: 328 PVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGS 387
            +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS
Sbjct: 327 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGS 386

Query: 388 NSSSPD-PSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILSNTAANG 447
                   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +G
Sbjct: 387 LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDG 446

Query: 448 EELVADCHLVPAVAVGEREGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAA 507
           E LVADCH++PA +VG   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+
Sbjct: 447 EGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVAS 506

Query: 508 FSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHV 567
           FS+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHV
Sbjct: 507 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 566

Query: 568 SGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRK 627
           SG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P K
Sbjct: 567 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 626

Query: 628 ALDPGLVYEIQPQDYFDFLCTQDLTPTQL-KVFSKYSNRTCHRLLPNPGDLNYPAISAVF 687
           A+DPGLVY+I   DY +FLC  + T T +  +  + ++    R   + G+LNYP+ S VF
Sbjct: 627 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVF 686

Query: 688 PE-KTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPESLNFTRRYEKLSYRITF 747
            +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  +KLS+ +  
Sbjct: 687 QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRV 746

Query: 748 LTKKRQSMP-----EFGGLIWKDGTHKVRSPIVIT 769
            T + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 KTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi05G012890.1 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 751.5 bits (1939), Expect = 5.1e-217
Identity = 396/773 (51.23%), Postives = 516/773 (66.75%), Query Frame = 1

Query: 9   LCLLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNLEREGSGGG 68
           L L L F     + +Q  KT++ ++D  +MP  F  H HWYST  +              
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  GEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPKFLGLEPAD 128
            E RI++ YH VFHG +A ++  EA+ L     VLA+F + + ELHTTRSP+FLGL+  +
Sbjct: 68  -ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--N 127

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGFTKQNCNRK 188
               WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRK
Sbjct: 128 QKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRK 187

Query: 189 IVGARVFYHGYQAAT-GKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTA 248
           I+GAR F  G QAA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G A
Sbjct: 188 IIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVA 247

Query: 249 RGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSL 308
           +G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D +
Sbjct: 248 KGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPI 307

Query: 309 SVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVT 368
           ++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  + 
Sbjct: 308 AIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLR 367

Query: 369 GVSLYRGRITIPEN-KQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGISPR 428
           GVSLY G   +P N + FPVVY G +  S   +SLC+E TLDP  V GKIVICDRG SPR
Sbjct: 368 GVSLYAG---VPLNGRMFPVVYPGKSGMSS--ASLCMENTLDPKQVRGKIVICDRGSSPR 427

Query: 429 VQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKATAT 488
           V KG+VVK AGGVGMIL+N A+NGE LV D HL+PA AVG  EG  IK YA ++    A+
Sbjct: 428 VAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIAS 487

Query: 489 LGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDT 548
           + F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D 
Sbjct: 488 IDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDP 547

Query: 549 RRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSG 608
           R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S 
Sbjct: 548 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDEST 607

Query: 609 ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTCHR 668
              ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R    
Sbjct: 608 GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTT 667

Query: 669 LLPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 728
             P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P 
Sbjct: 668 RKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPP 727

Query: 729 SLNFTRRYEKLSYRITFLTKKR-----QSMPEFGGLIWKD-GTHKVRSPIVIT 769
            L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 RLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Lsi05G012890.1 vs. NCBI nr
Match: gi|778679387|ref|XP_004148149.2| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1486.5 bits (3847), Expect = 0.0e+00
Identity = 727/773 (94.05%), Postives = 754/773 (97.54%), Query Frame = 1

Query: 1   MADTPLKWLCLLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNL 60
           MAD+PLK LC LLF   FLLS+A F KTYVVQMD+SAMPDSFT+H  WYS VL+NVV++L
Sbjct: 35  MADSPLKCLCFLLFLDSFLLSSALFLKTYVVQMDRSAMPDSFTNHFEWYSNVLTNVVLDL 94

Query: 61  EREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPK 120
           +REG+GGGGEERIIY YHNVFHG+AARLSE+E EKLEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 95  QREGNGGGGEERIIYGYHNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 154

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESDSF+DAGMSPVPAHWKGECETGRGF
Sbjct: 155 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 214

Query: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFY GYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 215 TKQNCNRKIVGARVFYRGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 274

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 275 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 334

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 335 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 394

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T+TGVSLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS
Sbjct: 395 TITGVSLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 454

Query: 421 PRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGG+GMILSNTAANGEELVADCHLVPAVA+GEREGKAIKQYALTNR+AT
Sbjct: 455 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEREGKAIKQYALTNRRAT 514

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 515 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 574

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS
Sbjct: 575 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 634

Query: 601 SGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660
           S ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDL+PTQLKVFSKYSNRTC
Sbjct: 635 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLSPTQLKVFSKYSNRTC 694

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEP 720
             LLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPATSSYHAVV+PF GA VKVEP
Sbjct: 695 RGLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPATSSYHAVVSPFKGATVKVEP 754

Query: 721 ESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWLSFV 774
           ESLNFTRRYEK+SYRITF+TKKRQSMPEFGGLIWKDG+HKVRSPIVITWLSFV
Sbjct: 755 ESLNFTRRYEKVSYRITFVTKKRQSMPEFGGLIWKDGSHKVRSPIVITWLSFV 807

BLAST of Lsi05G012890.1 vs. NCBI nr
Match: gi|659077296|ref|XP_008439131.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 729/773 (94.31%), Postives = 751/773 (97.15%), Query Frame = 1

Query: 1   MADTPLKWLCLLLFFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVNL 60
           MAD PLK LCLLLF    LLS+A F KTYVVQMD+SAMP SFT+H  WYS VLSNVVV+L
Sbjct: 35  MADFPLKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 94

Query: 61  EREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSPK 120
           EREG+GGGGEERIIYSY NVFHG+AARLSE+E EKLEEEDGV+AIFPE KYELHTTRSP+
Sbjct: 95  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 154

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESDSF+DAGMSPVPAHWKGECETGRGF
Sbjct: 155 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 214

Query: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 215 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 274

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 275 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 334

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 335 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 394

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSSSPDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 395 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 454

Query: 421 PRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGG+GMILSNT ANGEELVADCHLVPAVAVGE+EGKAIKQYALTNRKAT
Sbjct: 455 PRVQKGVVVKNAGGIGMILSNTVANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 514

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 515 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 574

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 575 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 634

Query: 601 SGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660
           S ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC
Sbjct: 635 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 694

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPF GAAVKVEP
Sbjct: 695 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 754

Query: 721 ESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWLSFV 774
           ESLNFTRRYEKLSYRITF+TKKR SMPEFGGLIWKDG+HKVRSPIV+TWLSFV
Sbjct: 755 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVMTWLSFV 807

BLAST of Lsi05G012890.1 vs. NCBI nr
Match: gi|596038840|ref|XP_007219861.1| (hypothetical protein PRUPE_ppa1027166mg [Prunus persica])

HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 610/777 (78.51%), Postives = 690/777 (88.80%), Query Frame = 1

Query: 1   MADT-PLKWLCLLL----FFHCFLLSTAQF-FKTYVVQMDKSAMPDSFTDHLHWYSTVLS 60
           MAD  P+KWL L+L    FF     +  QF  KTY+VQMDKSA P+SFT+HL WYS+ ++
Sbjct: 1   MADQKPVKWLVLILTNCLFFSIAFSAKTQFAHKTYIVQMDKSAKPESFTNHLDWYSSKVN 60

Query: 61  NVVVNLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELH 120
           ++V   E E  GG  +ER+IY+Y N FHG+AARLSE+EAE+L+E+DGVLAIFP+TKY+LH
Sbjct: 61  SIVFKPENEEDGGHDQERVIYTYQNAFHGVAARLSEEEAERLQEQDGVLAIFPDTKYQLH 120

Query: 121 TTRSPKFLGLEPADSNSA-WSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGE 180
           TTRSP FLGLEP DS +  WSQ++ DHDVIVGVLDTG+WPES SFND GMSPVPA+WKG 
Sbjct: 121 TTRSPLFLGLEPHDSTTTVWSQRVTDHDVIVGVLDTGVWPESQSFNDTGMSPVPAYWKGA 180

Query: 181 CETGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSP 240
           CETGRGF+K NCN+KIVGAR+FYHGY+AATGK NEQ E+KSPRDQDGHGTHTAATVAGSP
Sbjct: 181 CETGRGFSKHNCNKKIVGARIFYHGYEAATGKINEQTEFKSPRDQDGHGTHTAATVAGSP 240

Query: 241 VAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGG 300
           V GA+LLGYA+GTARGMAPGARIAAYKVCWVGGCFSSDILSAVD+AVADGVNVLSISLGG
Sbjct: 241 VRGANLLGYAHGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDKAVADGVNVLSISLGG 300

Query: 301 GVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 360
           GVS+YYRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP+ 
Sbjct: 301 GVSAYYRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPSS 360

Query: 361 VKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIV 420
           VKLG+GRTVTGVSLY+GR+ +  NKQ+PVVYMG NS+SPDPSSLCLEGTLD   VAGKIV
Sbjct: 361 VKLGNGRTVTGVSLYKGRMMLSTNKQYPVVYMGDNSTSPDPSSLCLEGTLDRRVVAGKIV 420

Query: 421 ICDRGISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYA 480
           ICDRGISPRVQKG VVK+AGGVGMIL+NTAANGEELVADCHLVPAVAVGE E KAIK YA
Sbjct: 421 ICDRGISPRVQKGQVVKDAGGVGMILANTAANGEELVADCHLVPAVAVGETEAKAIKHYA 480

Query: 481 LTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 540
           LT+ +ATATL FLGTR GV+PSPVVAAFSSRGPNF++LEILKPD+VAPGVNILAAWTG  
Sbjct: 481 LTSPRATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSLEILKPDVVAPGVNILAAWTGAL 540

Query: 541 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 600
           GPSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT
Sbjct: 541 GPSSLPTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNT 600

Query: 601 YKPLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFS 660
           +KPL+D+S A  S+PYDHGAGHINPRKALDPGLVY+I+ QDY +FLCTQ LTP QLKVF+
Sbjct: 601 HKPLQDASAAEASTPYDHGAGHINPRKALDPGLVYDIEAQDYLEFLCTQRLTPMQLKVFT 660

Query: 661 KYSNRTCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNG 720
           KYSNR+C   L +PGDLNYPAIS VFPE+T+V+ LTLHRTVTNVGP  S+YHA+V+PF G
Sbjct: 661 KYSNRSCKHALASPGDLNYPAISVVFPERTNVSLLTLHRTVTNVGPPVSNYHAIVSPFKG 720

Query: 721 AAVKVEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           A VKVEP +L FTR  +KLSY+ITF TK RQ+ PEFGGL+WKDG H+VRSPIV+ WL
Sbjct: 721 AYVKVEPRTLKFTRANQKLSYKITFTTKSRQATPEFGGLVWKDGVHRVRSPIVVVWL 777

BLAST of Lsi05G012890.1 vs. NCBI nr
Match: gi|645257258|ref|XP_008234331.1| (PREDICTED: subtilisin-like protease [Prunus mume])

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 606/772 (78.50%), Postives = 685/772 (88.73%), Query Frame = 1

Query: 5   PLKWLCLLL----FFHCFLLSTAQF-FKTYVVQMDKSAMPDSFTDHLHWYSTVLSNVVVN 64
           P+KWL L+L    FF     +  QF  KTY+VQMDKSA P+SFT+HL WYS+ ++++V  
Sbjct: 67  PVKWLVLILTNCLFFSIAFSAKTQFAHKTYIVQMDKSAKPESFTNHLDWYSSKVNSIVFK 126

Query: 65  LEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELHTTRSP 124
            E E  GG  +ER+IY+Y N FHG+AARLSE+EAE+L+E+DGVLAIFP+TKY+LHTTRSP
Sbjct: 127 PENEEDGGHNQERVIYAYQNAFHGVAARLSEEEAERLQEQDGVLAIFPDTKYQLHTTRSP 186

Query: 125 KFLGLEPADSNS-AWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGECETGR 184
            FLGLEP DS +  WSQ++ DHDVIVGVLDTG+WPES SFND GMSPVPA WKG CETGR
Sbjct: 187 LFLGLEPHDSTTNVWSQRVTDHDVIVGVLDTGVWPESQSFNDTGMSPVPARWKGACETGR 246

Query: 185 GFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 244
           GF+K NCN+KIVGAR+FY GY+AATGK NEQ E+KSPRDQDGHGTHTAATVAGSPV GA+
Sbjct: 247 GFSKHNCNKKIVGARIFYQGYEAATGKINEQTEFKSPRDQDGHGTHTAATVAGSPVRGAN 306

Query: 245 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 304
           LLGYA+GTARGMAPGARIAAYKVCWVGGCFSSDILSAVD+AVADGVNVLSISLGGGVS+Y
Sbjct: 307 LLGYAHGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDKAVADGVNVLSISLGGGVSAY 366

Query: 305 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGD 364
           YRDSLS+AAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFP+ VKLG+
Sbjct: 367 YRDSLSIAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPSTVKLGN 426

Query: 365 GRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVICDRG 424
           GRTVTGVSLY+G + +  NKQ+PVVYMG+NS+SPDPSSLCLEGTLD   VAGKIVICDRG
Sbjct: 427 GRTVTGVSLYKGTMMLSTNKQYPVVYMGNNSTSPDPSSLCLEGTLDRRVVAGKIVICDRG 486

Query: 425 ISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYALTNRK 484
           ISPRVQKG VVK+AGGVGMIL+NTAANGEELVADCHLVPAVAVGE E KAIK YALT+ +
Sbjct: 487 ISPRVQKGQVVKDAGGVGMILANTAANGEELVADCHLVPAVAVGETEAKAIKHYALTSPR 546

Query: 485 ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 544
           ATATL FLGTR GV+PSPVVAAFSSRGPNF++LEILKPD+VAPGVNILAAWTG  GPSSL
Sbjct: 547 ATATLAFLGTRTGVRPSPVVAAFSSRGPNFVSLEILKPDVVAPGVNILAAWTGALGPSSL 606

Query: 545 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 604
            TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT+KPL+
Sbjct: 607 PTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNTHKPLQ 666

Query: 605 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNR 664
           D+S A  S+PYDHGAGHINPRKALDPGLVY+I+ QDY +FLCTQ LTP QLKVF+KYSNR
Sbjct: 667 DASAAEASTPYDHGAGHINPRKALDPGLVYDIEAQDYLEFLCTQRLTPMQLKVFTKYSNR 726

Query: 665 TCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKV 724
           +C   L +PGDLNYPAIS VFPE+T+V+ LTLHRTVTNVGP  S+YHA+V+PF GA VKV
Sbjct: 727 SCKHSLASPGDLNYPAISVVFPERTNVSLLTLHRTVTNVGPPVSNYHAIVSPFKGAYVKV 786

Query: 725 EPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           EP +L FTR  +KLSY+ITF TK RQ+ PEFGGL+WKDG H+VRSPIVI WL
Sbjct: 787 EPRTLKFTRANQKLSYKITFTTKSRQATPEFGGLVWKDGVHRVRSPIVIVWL 838

BLAST of Lsi05G012890.1 vs. NCBI nr
Match: gi|590672084|ref|XP_007038510.1| (Subtilase 1.3 [Theobroma cacao])

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 606/777 (77.99%), Postives = 681/777 (87.64%), Query Frame = 1

Query: 1   MADTPLKWLCLLL-----FFHCFLLSTAQFFKTYVVQMDKSAMPDSFTDHLHWYSTVLSN 60
           M + P+KWL L+L     F      S     KT++VQMDKSAMP SF+ HL WYS+ + +
Sbjct: 1   MTENPVKWLFLILTSCLSFALVLSASNPLIKKTFIVQMDKSAMPASFSSHLEWYSSKVKS 60

Query: 61  VVV-NLEREGSGGGGEERIIYSYHNVFHGIAARLSEQEAEKLEEEDGVLAIFPETKYELH 120
           V++ N + EG G G  ERIIYSY N FHG+AA+L+E EAE+LEEEDGV+AI PE KY+LH
Sbjct: 61  VIMSNTQSEGDGDG--ERIIYSYQNAFHGVAAQLTEDEAERLEEEDGVVAILPEMKYQLH 120

Query: 121 TTRSPKFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESDSFNDAGMSPVPAHWKGEC 180
           TTRSP FLGLEP +S S WSQ++ DHDVIVGVLDTGIWPES+SFND G++PVPAHWKG C
Sbjct: 121 TTRSPMFLGLEPEESTSIWSQKLTDHDVIVGVLDTGIWPESESFNDTGLAPVPAHWKGAC 180

Query: 181 ETGRGFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPV 240
           ETGRGF K +CNRKIVGARVFY GY+AATGK NE+ EYKSPRDQDGHGTHTAATVAGSPV
Sbjct: 181 ETGRGFEKHHCNRKIVGARVFYRGYEAATGKINEKNEYKSPRDQDGHGTHTAATVAGSPV 240

Query: 241 AGASLLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGG 300
            GA+LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGV+VLSISLGGG
Sbjct: 241 RGANLLGYAYGTARGMAPGARIAAYKVCWTGGCFSSDILSAVDRAVADGVSVLSISLGGG 300

Query: 301 VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIV 360
           VSSYYRDSL++A FGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPA V
Sbjct: 301 VSSYYRDSLAIATFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPADV 360

Query: 361 KLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSNSSSPDPSSLCLEGTLDPHFVAGKIVI 420
           KLG GRT+TGVSLY+G+  +  NKQ+P+VYMGSNSSSPDPSSLCLEGTLDPH V+GKIVI
Sbjct: 361 KLGTGRTLTGVSLYKGQRFLSPNKQYPIVYMGSNSSSPDPSSLCLEGTLDPHIVSGKIVI 420

Query: 421 CDRGISPRVQKGVVVKNAGGVGMILSNTAANGEELVADCHLVPAVAVGEREGKAIKQYAL 480
           CDRGISPRVQKG VVK+AGG+GMIL+NTAANGEELVADCHL+PA+AVGE EGKAIK YAL
Sbjct: 421 CDRGISPRVQKGQVVKDAGGIGMILTNTAANGEELVADCHLLPALAVGEMEGKAIKHYAL 480

Query: 481 TNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTG 540
           T+RKATATL FLGTRLG++PSPVVAAFSSRGPNFLT EILKPD+VAPGVNILAAWTG+ G
Sbjct: 481 TSRKATATLAFLGTRLGIRPSPVVAAFSSRGPNFLTFEILKPDMVAPGVNILAAWTGELG 540

Query: 541 PSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTY 600
           PSSL TD RRVKFNILSGTSMSCPHVSG+AAL+K++HP+WSP+AIKSALMTTAYVHDNT+
Sbjct: 541 PSSLQTDHRRVKFNILSGTSMSCPHVSGIAALLKARHPEWSPAAIKSALMTTAYVHDNTH 600

Query: 601 KPLKDSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSK 660
            PLKD++ A+ S+PYDHGAGHINP KALDPGLVY+I+ QDYF+FLCTQ LT  QLKVF K
Sbjct: 601 NPLKDAAEAAISTPYDHGAGHINPLKALDPGLVYDIEAQDYFEFLCTQKLTTMQLKVFGK 660

Query: 661 YSNRTCHRLLPNPGDLNYPAISAVFPEKTSVTS-LTLHRTVTNVGPATSSYHAVVTPFNG 720
           YSNR CH  L + GDLNYPAIS VFPE T+  S LTLHRTVTNVGP  S+YH VV+ F G
Sbjct: 661 YSNRFCHHTLASAGDLNYPAISVVFPEDTTAISVLTLHRTVTNVGPPISNYHVVVSQFKG 720

Query: 721 AAVKVEPESLNFTRRYEKLSYRITFLTKKRQSMPEFGGLIWKDGTHKVRSPIVITWL 771
           A VKV+P+SLNFTR+ +KLSY+ITF TK  Q++PEFGGL+WKDG HKVRSPIVITW+
Sbjct: 721 ATVKVDPKSLNFTRKNQKLSYKITFTTKSPQTVPEFGGLVWKDGVHKVRSPIVITWI 775