Lsi02G026420.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G026420.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Serine/threonine-protein kinase TIO
Location	chr02 : 32851578 .. 32852879 (-)
Sequence length	1008

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTAAATTGATCGAATAAATTACTCTACAATTTAACAAAGAATATTATAATCTCTAGCTTAATTAATCATCCAATTTAATTTCTTACTTAATTTTCTCTCTGCCCTAAACCTAGGGTTATACTAAACTTTTGCCTCTTCTTTCAAATTTTGTTGGTTAATTATACTAATTTATCTAACCCTAACGTTGATAATTTCATACTAAGTATTGATTTCCACTTACTAATATAATAGTGTAAAATTTTGGTGGTTTTCTTAGAAAATTTAATTTATTGGTTGCTGATCTTATTTAAGCAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

mRNA sequence

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

Coding sequence (CDS)

ATGAAGAGAAATTCAATGGAAGAAGCTGAAGCAGAAGAAACCCTATTTATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAGCGAGGGCAGCTCATCGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACCATTTTCCCTCCGATAATGGCCGTTAAATCCGCTGAAATTTCCGTCTCCAAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCTTTGATCCAATGCTTCGCCGAAGAGATTACTACCGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTTGCCGCTGGAGGAACCCTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAGGTTCGGCGTTACACAAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCAAATATCTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAATCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTGACCGGAAACCTCCCGTGGACCGCCGCGGACAAGGCCGGGATTGTGAAACAGATCACGGAGGATTACGGAATGCCAAAGATTCCGGAAGGGTTGTCGGCGGAGGCAAGTGGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGGCGTTCAGGTTCACAGCGGAGATGCTGATGACTGTGCCGTTTGTGGCAGGGATTGAAGATCAGGAAGAAGATCAAGATTTTGTGGGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTGA

Protein sequence

MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKAGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV

Homology

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BLAST of Lsi02G026420.1 vs. Swiss-Prot
Match: MKKA_DICDI (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=mkkA PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 7.0e-32
Identity = 91/296 (30.74%), Postives = 149/296 (50.34%), Query Frame = 1


Query: 24  NNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYD 83
           N+ I+W++GQ++G+G +GSV+L   K           + AVK  EI    +  K K    
Sbjct: 165 NHNIKWQKGQILGRGGYGSVYLGLNKDT-------GELFAVKQLEIVDINSDPKLK---- 224

Query: 84  NLKGCNSLIQCFAEEI----TTDHNGHMIY----------NLLLEVAAGGTLAHHINNTG 143
                 ++I  F++EI    +  H+  + Y          ++ LE   GG+++  +   G
Sbjct: 225 ------NMILSFSKEIEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFG 284

Query: 144 GKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGL 203
                EN ++ YTK I++GL  +H N  +H D+K ANIL+     TKG   + K++D G 
Sbjct: 285 A--FSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKG---IVKLSDFGC 344

Query: 204 ARRISKTKPSY-CLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWT-AADK 263
           ++  S     +  + GT  +MAPE +  +     SDIW+LGC++++M T   PW+   + 
Sbjct: 345 SKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITEL 404

Query: 264 AGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIED 304
           A ++  I     +P IP  +S EA  FL  CF R+P  R  A  L+  PF+  ++D
Sbjct: 405 AAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDD 434

[Close]

BLAST of Lsi02G026420.1 vs. Swiss-Prot
Match: M3K2_ARATH (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.7e-30
Identity = 91/310 (29.35%), Postives = 154/310 (49.68%), Query Frame = 1


Query: 15  LFITSSSSFNNG-------IQWERGQLIGKGSFGSVFLA----SRKPHISKYTIFPPIMA 74
           +F  S S  NN        I+W +GQLIG+G+FG+V++     S +    K  +     A
Sbjct: 47  VFAKSQSPPNNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCA 106

Query: 75  VKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAH 134
            K    +  + L++E +   NL   N  I  +   +  D       N+LLE   GG+++ 
Sbjct: 107 SKEKTQAHIQELEEEVKLLKNLSHPN--IVRYLGTVREDET----LNILLEFVPGGSISS 166

Query: 135 HINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAK 194
            +   G    PE+ VR YT  ++ GL ++H +  +H D+K ANIL+         +   K
Sbjct: 167 LLEKFGA--FPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILV-------DNQGCIK 226

Query: 195 IADLGLARRISKTKP---SYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNL 254
           +AD G ++++++      +  + GT  +MAPE ++ +     +DIW++GC V++M+TG  
Sbjct: 227 LADFGASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKA 286

Query: 255 PWTAADK-AGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVA 310
           PW+   K    +  I      P IP+ +S++A+ FL  C  + P  R TA  L+  PFV 
Sbjct: 287 PWSQQYKEIAAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVT 341

[Close]

BLAST of Lsi02G026420.1 vs. Swiss-Prot
Match: M3K3_ARATH (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.6e-30
Identity = 87/282 (30.85%), Postives = 140/282 (49.65%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLA----SRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNY 86
           I+W +G+LIG G+FG V++     S +    K  +  P  A K       + L++E Q  
Sbjct: 66  IRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLL 125

Query: 87  DNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYT 146
            NL   N +++       +D       N+L+E   GG+++  +   G    PE  +  YT
Sbjct: 126 KNLSHPN-IVRYLGTVRESDS-----LNILMEFVPGGSISSLLEKFGS--FPEPVIIMYT 185

Query: 147 KSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRI---SKTKPS 206
           K ++ GL ++H N  +H D+K ANIL+      KG     ++AD G ++++   +    +
Sbjct: 186 KQLLLGLEYLHNNGIMHRDIKGANILV----DNKG---CIRLADFGASKKVVELATVNGA 245

Query: 207 YCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAA-DKAGIVKQITEDY 266
             + GT  +MAPE ++ +     +DIW++GC V++M TG  PW+    +   V  I    
Sbjct: 246 KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTK 305

Query: 267 GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAG 301
             P IPE LS EA  FL  C  + P+ R +A  L+  PFV G
Sbjct: 306 AHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTG 332

[Close]

BLAST of Lsi02G026420.1 vs. Swiss-Prot
Match: YODA_ARATH (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana GN=YDA PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.9e-29
Identity = 90/289 (31.14%), Postives = 144/289 (49.83%), Query Frame = 1


Query: 19  SSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVS-KTLQK 78
           + ++ + G +W++G+L+G GSFG V+L           +    +     +   S + L +
Sbjct: 390 AEATVSPGSRWKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQ 449

Query: 79  EKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENE 138
           E      L+  N ++Q +  E   D     +Y + LE  +GG++   +   G  G  EN 
Sbjct: 450 EISVLSRLRHQN-IVQYYGSETVDDK----LY-IYLEYVSGGSIYKLLQEYGQFG--ENA 509

Query: 139 VRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTK 198
           +R YT+ I+ GL ++H    VH D+K ANIL+ P     GR    K+AD G+A+ I+   
Sbjct: 510 IRNYTQQILSGLAYLHAKNTVHRDIKGANILVDPH----GR---VKVADFGMAKHITAQS 569

Query: 199 PSYCLGGTLSYMAPETLIDSVQESPS-DIWALGCLVLQMLTGNLPWTAADKAGIVKQITE 258
                 G+  +MAPE + +S   + + DIW+LGC VL+M T   PW+  +    + +I  
Sbjct: 570 GPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGN 629

Query: 259 DYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQE 306
              +P IP+ LS E   F++ C  RNPA R TA  L+   FV  +   E
Sbjct: 630 SKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFVRNVMPME 663

[Close]

BLAST of Lsi02G026420.1 vs. Swiss-Prot
Match: ANP1_ARATH (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=ANP1 PE=1 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 3.3e-29
Identity = 89/291 (30.58%), Postives = 145/291 (49.83%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLA----SRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNY 86
           I W +GQLIG+G+FG+V++     S +    K  +     A K    +  + L++E +  
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 87  DNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYT 146
            NL   N  I  +   +  D       N+LLE   GG+++  +   G    PE+ VR YT
Sbjct: 127 KNLSHPN--IVRYLGTVREDDT----LNILLEFVPGGSISSLLEKFGP--FPESVVRTYT 186

Query: 147 KSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKP---S 206
           + ++ GL ++H +  +H D+K ANIL+      KG     K+AD G ++++++      +
Sbjct: 187 RQLLLGLEYLHNHAIMHRDIKGANILV----DNKG---CIKLADFGASKQVAELATMTGA 246

Query: 207 YCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADK-AGIVKQITEDY 266
             + GT  +MAPE ++ +     +DIW++GC V++M+TG  PW+   K    +  I    
Sbjct: 247 KSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTK 306

Query: 267 GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQD 310
             P IP+ LS++A  FL  C    P  R TA  L+  PFV G   +    D
Sbjct: 307 SHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTD 342

[Close]

BLAST of Lsi02G026420.1 vs. TrEMBL
Match: A0A0A0KBM3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 3.5e-147
Identity = 266/337 (78.93%), Postives = 290/337 (86.05%), Query Frame = 1


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR SM E EA +      SS FNNGIQW++G+LIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIVK++TE++ GMPKIPEGLS EA+GFLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of Lsi02G026420.1 vs. TrEMBL
Match: A0A0A0K663_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430790 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 9.9e-142
Identity = 261/341 (76.54%), Postives = 290/341 (85.04%), Query Frame = 1


Query: 1   MKRNSME---EAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYT 60
           MKRN      E E E+      SS FNNG+QW+RG+LIGKGSFGSVFLAS KPHI+ KY 
Sbjct: 1   MKRNLTLGGLEVEVED-----GSSEFNNGVQWKRGRLIGKGSFGSVFLASLKPHITIKYC 60

Query: 61  IFPPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVA 120
            FP +MAVKSAEISVS+TLQKEKQNYD+LKGCNSLI+CF EEITTDHNGHMIYNLLLEVA
Sbjct: 61  TFPSVMAVKSAEISVSETLQKEKQNYDSLKGCNSLIKCFGEEITTDHNGHMIYNLLLEVA 120

Query: 121 AGGTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTT 180
            GGTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT
Sbjct: 121 TGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTT 180

Query: 181 KGRRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQM 240
           K R+F+AKIADLGLARR SKTK SYCLGGT SYMAPET ID VQES SDIWALGC+VL+M
Sbjct: 181 KDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETFIDGVQESASDIWALGCVVLEM 240

Query: 241 LTGNLPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMT 300
           LTGN  W A +K GI+K++TE++ GMPKIPEGLSAEA+ FLKNCFVR P FRFTAEMLM 
Sbjct: 241 LTGNRAWAATNKVGIMKEMTENFLGMPKIPEGLSAEATMFLKNCFVRKPEFRFTAEMLMI 300

Query: 301 VPFVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           VPFVA +EDQE++ + V AP+FV K P QFKRQRIIPIKAV
Sbjct: 301 VPFVAAVEDQEQNFNTVKAPTFVTKWPMQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420.1 vs. TrEMBL
Match: A0A124SGD8_CYNCS (Protein kinase, ATP binding site-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_015563 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 8.8e-82
Identity = 160/319 (50.16%), Postives = 213/319 (66.77%), Query Frame = 1


Query: 2   KRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPI 61
           KR+ + +  +E        S++ +G+ W RG LIGKG FGSVFLA+ K   S+Y+ +PPI
Sbjct: 12  KRSLITDENSERKRRKQEHSNYGDGMAWFRGSLIGKGCFGSVFLANLKKPKSRYSSYPPI 71

Query: 62  MAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTL 121
           MAVKSAE+SVS ++QKE++  +N++GC ++I+CF EEITT  NG M+YNLLLE  +GGTL
Sbjct: 72  MAVKSAEVSVSSSIQKEREVLNNIRGCRNVIRCFGEEITTGENGQMVYNLLLEYGSGGTL 131

Query: 122 AHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFV 181
           A  I  + G GLPE + RR+T+SI++GL HIH   YVHCDLKP NILLV  +   G  F 
Sbjct: 132 ADLIKKSDGNGLPEFDARRHTRSILRGLRHIHHCGYVHCDLKPENILLVASSVDGG--FT 191

Query: 182 AKIADLGLARRISKTKPSYCLG---GTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTG 241
           AKI DLGLA+R  ++K S  +    GT  Y +PE +ID VQE PSDIW +GC++L+MLTG
Sbjct: 192 AKIGDLGLAKRAKQSKKSKSVSYWRGTPMYFSPEVVIDGVQEPPSDIWTIGCVLLEMLTG 251

Query: 242 NLPWTAADKAG---IVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTV 301
             PW +  +     I+ +I E+   P IP  +S EA  FLK CF R   +R+TAEML+  
Sbjct: 252 KPPWDSKPETNGDEILSRIGENNESPSIPTTISREARSFLKGCFSRKAMYRWTAEMLLAH 311

Query: 302 PFVAGIEDQEEDQDFVGAP 315
           PF+ GI D E+D   V  P
Sbjct: 312 PFLEGIGDDEDDDHKVEEP 328

[Close]

BLAST of Lsi02G026420.1 vs. TrEMBL
Match: A0A022PZJ4_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a021677mg PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 5.7e-81
Identity = 161/295 (54.58%), Postives = 198/295 (67.12%), Query Frame = 1


Query: 21  SSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQ 80
           S + +G  W RG +IGKG FGSV+LA+ K   SKY+ FPP+MAVKSAE+S S ++QKEK+
Sbjct: 4   SEYGDGAAWFRGSIIGKGGFGSVYLATLKNPTSKYSCFPPLMAVKSAEVSASGSIQKEKE 63

Query: 81  NYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRR 140
              NLKGC  LI+CF EE T   NG M YNLLLE  +GGTL+  I  +GG GL E E R 
Sbjct: 64  VLTNLKGCPYLIKCFGEETTIGSNGVMAYNLLLEYGSGGTLSDRIEKSGGGGLAECEARV 123

Query: 141 YTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSY 200
           YT+SI++GL HIH N YVHCDLKP NILLVP +   G    AKI D GLA+R   +K S 
Sbjct: 124 YTRSILRGLNHIHGNGYVHCDLKPENILLVPNS---GAGIRAKIGDFGLAKRERNSKKSK 183

Query: 201 CL----GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKAGIVKQITE 260
            L     GT  Y++PE ++D VQESPSDIWALGC+VL MLTGNLPW       I+++I  
Sbjct: 184 ILEPYWRGTPMYLSPEAVVDKVQESPSDIWALGCIVLYMLTGNLPWEGKKSEEIIRKIGT 243

Query: 261 DYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFV 312
              +PKIP  LS EA  FLK CFVR   FR T+EML+  PFV  +E+ +  ++ V
Sbjct: 244 RGRLPKIPNELSKEAKDFLKGCFVRRSVFRHTSEMLLHHPFVEVLENGDAVEESV 295

[Close]

BLAST of Lsi02G026420.1 vs. TrEMBL
Match: A0A068U8R3_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00018548001 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.3e-80
Identity = 157/294 (53.40%), Postives = 205/294 (69.73%), Query Frame = 1


Query: 21  SSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQ 80
           + + +G+ W+RG ++GKGSFGSV+LA+ K   SKY+  P +MAVKSAE+SVS ++QKE++
Sbjct: 4   NKYGDGVAWKRGAMLGKGSFGSVYLATLKKPRSKYSCLPSVMAVKSAEVSVSGSIQKERE 63

Query: 81  NYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRR 140
              N+KGC ++IQCF EE TT  NG M++NLLLE  +GGTLA  I  +GG GLPE EVR 
Sbjct: 64  VLSNVKGCPNIIQCFGEETTTGVNGVMVFNLLLEYGSGGTLAEKIKKSGGNGLPEFEVRC 123

Query: 141 YTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRR-FVAKIADLGLARRI---SKT 200
           YT+SI+KGL  IH+  YVHCDLKP NILLVP N   GR  F AKI D GLA+R+    K 
Sbjct: 124 YTRSILKGLNFIHEIGYVHCDLKPENILLVPNNAGGGRAGFRAKIGDFGLAKRVKQCKKR 183

Query: 201 KPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKAG---IVKQ 260
           K      GT +Y++PE + D VQE PSDIWALGC+VL+MLTG  PW A ++     I+K+
Sbjct: 184 KLEPYWRGTPTYLSPEVVGDGVQEQPSDIWALGCIVLEMLTGKPPWDAKEEQNAEDILKR 243

Query: 261 ITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEED 308
           I  +  +P+IP  LS EA  FLK CFV+   +R TAEML+   F+ G+ D ++D
Sbjct: 244 IKNE--LPQIPSELSKEAKDFLKGCFVKKAMYRLTAEMLLNHAFMEGLVDGDDD 295

[Close]

BLAST of Lsi02G026420.1 vs. TAIR10
Match: AT5G67080.1 (AT5G67080.1 mitogen-activated protein kinase kinase kinase 19)

HSP 1 Score: 210.3 bits (534), Expect = 1.8e-54
Identity = 122/295 (41.36%), Postives = 179/295 (60.68%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNL- 86
           ++W RG+ IG G+F +V LA+R  + S    FPP+MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGE--FPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 87  KGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSI 146
             CN +++CF E+ T + NG  ++NL LE A+ G+L  ++    G+G+PE+ VRR+T S+
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 147 IKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISK-TKPSY--CL 206
           ++GL HIH N + HCDLK  NILL             KIAD GLA+RI   T  +Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDG-------AVKIADFGLAKRIGDLTALNYGVQI 180

Query: 207 GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA---GIVKQITEDYG 266
            GT  YMAPE++ D+   S  D+WALGC+V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDE 240

Query: 267 MPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQE---EDQDFV 312
           +P IPE LS +   FL  CFV++P  R+TAEML+  PFV    D +   +++DFV
Sbjct: 241 VPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 285

[Close]

BLAST of Lsi02G026420.1 vs. TAIR10
Match: AT4G36950.1 (AT4G36950.1 mitogen-activated protein kinase kinase kinase 21)

HSP 1 Score: 209.5 bits (532), Expect = 3.1e-54
Identity = 121/288 (42.01%), Postives = 174/288 (60.42%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W R + IG GSF +V LA+     SK   FP +MAVKS+ +  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
            C+ +++CF E  T + NG  IYNL LE A+GG+LA  I ++G + LPE EVRR+T+SI+
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSSG-EALPEFEVRRFTRSIV 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTL 206
           KGL HIH N + HCD+K  N+L+             KI+D GLA+R S  +    + GT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKRRS-GEVCVEIRGTP 180

Query: 207 SYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA-----GIVKQITEDYGMP 266
            YMAPE++     ESP+DIWALGC V++M +G   W   D        ++ +I     +P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 267 KIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQD 310
           +IP  LS E   F+  CFV+N A R+TAEML+  PF+A ++D+  ++D
Sbjct: 241 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLA-VDDESGEED 275

[Close]

BLAST of Lsi02G026420.1 vs. TAIR10
Match: AT3G50310.1 (AT3G50310.1 mitogen-activated protein kinase kinase kinase 20)

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-53
Identity = 115/292 (39.38%), Postives = 175/292 (59.93%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           ++W RG+ IG G+F +V  A++  +      FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VRR+T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRI----SKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    + K       IAD GLA R+    +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGSVK-------IADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAADKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S +D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQDFV 312
           +PKIPE LS E   FL  CFV++PA R+TAEML+   FV    + +  ++FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

[Close]

BLAST of Lsi02G026420.1 vs. TAIR10
Match: AT4G26890.1 (AT4G26890.1 mitogen-activated protein kinase kinase kinase 16)

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 105/283 (37.10%), Postives = 157/283 (55.48%), Query Frame = 1


Query: 27  IQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSKTLQKEKQNYDNLK 86
           I W RG +IG+GS  +V +A              + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSG--------ELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRRYTKSII 146
             + +++     +T + NG ++YN+L+E  +GG L   I N+GGK LPE E+R YT+ I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRISKTKPSYCLGGTL 206
            GL+++H+   VHCDLK  N+L+           V KIAD+G A+ + K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLVEENG-------VLKIADMGCAKSVDKSE----FSGTP 182

Query: 207 SYMAPETLIDSVQESPSDIWALGCLVLQMLTGNLPWTAA-DKAGIVKQITEDYGMPKIPE 266
           ++MAPE      Q  P+D+WALGC +++M+TG+ PW    D    + +I      P IP 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPA 242

Query: 267 GLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFVAGIEDQEEDQ 309
            +S +A  FLKNC   +   R+T E L+  PF   ++D EE Q
Sbjct: 243 WISDKAKDFLKNCLKEDQKQRWTVEELLKHPF---LDDDEESQ 260

[Close]

BLAST of Lsi02G026420.1 vs. TAIR10
Match: AT5G27510.1 (AT5G27510.1 Protein kinase superfamily protein)

HSP 1 Score: 168.3 bits (425), Expect = 8.0e-42
Identity = 96/242 (39.67%), Postives = 137/242 (56.61%), Query Frame = 1


Query: 63  AVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITT--DHNGHMIYNLLLEVAAGGT 122
           AVKS++   S  L KE      LKGC  +IQCF  ++    D  G+ +Y LLLE A+ G+
Sbjct: 35  AVKSSDDENS--LLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 123 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRF 182
           L+  +NN   + LP+  +R +T+ I++GL+ IH + YVHCDLKP N+L+ P     G  +
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFP----CGDSY 154

Query: 183 VAKIADLGLARRISKT----KPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQML 242
             KI+D GL+ ++ +     K  Y   GTL+YM PE+L D V     D+W+LGCLVL+M 
Sbjct: 155 EVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMY 214

Query: 243 TGNLPWTAADKAGIVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 299
               PW        V  I  +   P+IPE L  +A  F++ CF RNP  R TA  L++  
Sbjct: 215 VCKKPWIGFIPEDFV-YILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELLSHR 269

[Close]

BLAST of Lsi02G026420.1 vs. NCBI nr
Match: gi|449436643|ref|XP_004136102.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 5.0e-147
Identity = 266/337 (78.93%), Postives = 290/337 (86.05%), Query Frame = 1


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR SM E EA +      SS FNNGIQW++G+LIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GIVK++TE++ GMPKIPEGLS EA+GFLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of Lsi02G026420.1 vs. NCBI nr
Match: gi|659122990|ref|XP_008461430.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-146
Identity = 264/337 (78.34%), Postives = 292/337 (86.65%), Query Frame = 1


Query: 1   MKRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN M E EA +      S+ FNNGIQW+RG+LIGKGSFGSVFLA  KPH +KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQVE--EGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVS+TLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVPFV 300
             W A DK GI++++TE++ GMPKIPEGLSAEA+GFLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of Lsi02G026420.1 vs. NCBI nr
Match: gi|659122992|ref|XP_008461431.1| (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 519.6 bits (1337), Expect = 4.0e-144
Identity = 266/339 (78.47%), Postives = 291/339 (85.84%), Query Frame = 1


Query: 1   MKRNSM-EEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNSM EE E E       SS FNNGIQW+RG+LIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVEVE---DGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVS+TLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARR SKTK SYCLGGT SYMAPETLID VQES SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLT 240

Query: 241 GNLPWTAADKAGIVKQITED-YGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTVP 300
           GN  W A DK GIVK++TE+ +GMPKIP+GLS EA+GFLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + + AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420.1 vs. NCBI nr
Match: gi|449436645|ref|XP_004136103.1| (PREDICTED: protein kinase byr2-like [Cucumis sativus])

HSP 1 Score: 511.1 bits (1315), Expect = 1.4e-141
Identity = 261/341 (76.54%), Postives = 290/341 (85.04%), Query Frame = 1


Query: 1   MKRNSME---EAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHIS-KYT 60
           MKRN      E E E+      SS FNNG+QW+RG+LIGKGSFGSVFLAS KPHI+ KY 
Sbjct: 1   MKRNLTLGGLEVEVED-----GSSEFNNGVQWKRGRLIGKGSFGSVFLASLKPHITIKYC 60

Query: 61  IFPPIMAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVA 120
            FP +MAVKSAEISVS+TLQKEKQNYD+LKGCNSLI+CF EEITTDHNGHMIYNLLLEVA
Sbjct: 61  TFPSVMAVKSAEISVSETLQKEKQNYDSLKGCNSLIKCFGEEITTDHNGHMIYNLLLEVA 120

Query: 121 AGGTLAHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTT 180
            GGTLAHHI NTGGKGL EN VR YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT
Sbjct: 121 TGGTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTT 180

Query: 181 KGRRFVAKIADLGLARRISKTKPSYCLGGTLSYMAPETLIDSVQESPSDIWALGCLVLQM 240
           K R+F+AKIADLGLARR SKTK SYCLGGT SYMAPET ID VQES SDIWALGC+VL+M
Sbjct: 181 KDRQFIAKIADLGLARRTSKTKASYCLGGTFSYMAPETFIDGVQESASDIWALGCVVLEM 240

Query: 241 LTGNLPWTAADKAGIVKQITEDY-GMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMT 300
           LTGN  W A +K GI+K++TE++ GMPKIPEGLSAEA+ FLKNCFVR P FRFTAEMLM 
Sbjct: 241 LTGNRAWAATNKVGIMKEMTENFLGMPKIPEGLSAEATMFLKNCFVRKPEFRFTAEMLMI 300

Query: 301 VPFVAGIEDQEEDQDFVGAPSFVKKRPKQFKRQRIIPIKAV 336
           VPFVA +EDQE++ + V AP+FV K P QFKRQRIIPIKAV
Sbjct: 301 VPFVAAVEDQEQNFNTVKAPTFVTKWPMQFKRQRIIPIKAV 336

[Close]

BLAST of Lsi02G026420.1 vs. NCBI nr
Match: gi|976921399|gb|KVI06091.1| (Protein kinase, ATP binding site-containing protein [Cynara cardunculus var. scolymus])

HSP 1 Score: 312.0 bits (798), Expect = 1.3e-81
Identity = 160/319 (50.16%), Postives = 213/319 (66.77%), Query Frame = 1


Query: 2   KRNSMEEAEAEETLFITSSSSFNNGIQWERGQLIGKGSFGSVFLASRKPHISKYTIFPPI 61
           KR+ + +  +E        S++ +G+ W RG LIGKG FGSVFLA+ K   S+Y+ +PPI
Sbjct: 12  KRSLITDENSERKRRKQEHSNYGDGMAWFRGSLIGKGCFGSVFLANLKKPKSRYSSYPPI 71

Query: 62  MAVKSAEISVSKTLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTL 121
           MAVKSAE+SVS ++QKE++  +N++GC ++I+CF EEITT  NG M+YNLLLE  +GGTL
Sbjct: 72  MAVKSAEVSVSSSIQKEREVLNNIRGCRNVIRCFGEEITTGENGQMVYNLLLEYGSGGTL 131

Query: 122 AHHINNTGGKGLPENEVRRYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFV 181
           A  I  + G GLPE + RR+T+SI++GL HIH   YVHCDLKP NILLV  +   G  F 
Sbjct: 132 ADLIKKSDGNGLPEFDARRHTRSILRGLRHIHHCGYVHCDLKPENILLVASSVDGG--FT 191

Query: 182 AKIADLGLARRISKTKPSYCLG---GTLSYMAPETLIDSVQESPSDIWALGCLVLQMLTG 241
           AKI DLGLA+R  ++K S  +    GT  Y +PE +ID VQE PSDIW +GC++L+MLTG
Sbjct: 192 AKIGDLGLAKRAKQSKKSKSVSYWRGTPMYFSPEVVIDGVQEPPSDIWTIGCVLLEMLTG 251

Query: 242 NLPWTAADKAG---IVKQITEDYGMPKIPEGLSAEASGFLKNCFVRNPAFRFTAEMLMTV 301
             PW +  +     I+ +I E+   P IP  +S EA  FLK CF R   +R+TAEML+  
Sbjct: 252 KPPWDSKPETNGDEILSRIGENNESPSIPTTISREARSFLKGCFSRKAMYRWTAEMLLAH 311

Query: 302 PFVAGIEDQEEDQDFVGAP 315
           PF+ GI D E+D   V  P
Sbjct: 312 PFLEGIGDDEDDDHKVEEP 328

The following BLAST results are available for this feature:

BLAST of Lsi02G026420.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MKKA_DICDI	7.0e-32	30.74	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=...	[more]
M3K2_ARATH	1.7e-30	29.35	Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2...	[more]
M3K3_ARATH	8.6e-30	30.85	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana GN=ANP3...	[more]
YODA_ARATH	1.9e-29	31.14	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana GN=Y...	[more]
ANP1_ARATH	3.3e-29	30.58	Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=A...	[more]

BLAST of Lsi02G026420.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBM3_CUCSA	3.5e-147	78.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430800 PE=3 SV=1	[more]
A0A0A0K663_CUCSA	9.9e-142	76.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430790 PE=3 SV=1	[more]
A0A124SGD8_CYNCS	8.8e-82	50.16	Protein kinase, ATP binding site-containing protein OS=Cynara cardunculus var. s...	[more]
A0A022PZJ4_ERYGU	5.7e-81	54.58	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a021677mg PE=3 SV=1	[more]
A0A068U8R3_COFCA	6.3e-80	53.40	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00018548001 PE=3 SV=1	[more]

BLAST of Lsi02G026420.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G67080.1	1.8e-54	41.36	mitogen-activated protein kinase kinase kinase 19	[more]
AT4G36950.1	3.1e-54	42.01	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	2.0e-53	39.38	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	5.9e-45	37.10	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G27510.1	8.0e-42	39.67	Protein kinase superfamily protein	[more]

BLAST of Lsi02G026420.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449436643\|ref\|XP_004136102.1\|	5.0e-147	78.93	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis sativu...	[more]
gi\|659122990\|ref\|XP_008461430.1\|	1.5e-146	78.34	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
gi\|659122992\|ref\|XP_008461431.1\|	4.0e-144	78.47	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
gi\|449436645\|ref\|XP_004136103.1\|	1.4e-141	76.54	PREDICTED: protein kinase byr2-like [Cucumis sativus]	[more]
gi\|976921399\|gb\|KVI06091.1\|	1.3e-81	50.16	Protein kinase, ATP binding site-containing protein [Cynara cardunculus var. sco...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0006468	protein phosphorylation

Vocabulary: **Molecular Function**
Term	Definition
GO:0005524	ATP binding
GO:0004672	protein kinase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR017441	Protein_kinase_ATP_BS
IPR011009	Kinase-like_dom_sf
IPR008271	Ser/Thr_kinase_AS
IPR000719	Prot_kinase_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0009069	serine family amino acid metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi02G026420	Lsi02G026420	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi02G026420.1.CDS.2	Lsi02G026420.1.CDS.2	CDS
Lsi02G026420.1.CDS.1	Lsi02G026420.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi02G026420.1	Lsi02G026420.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 30..297 score: 2.3
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 29..298 score: 4.2
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 29..298 score: 36
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 157..169 scor
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 28..305 score: 7.02
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 35..65 scor
None	No IPR available	GENE3D	G3DSA:1.10.510.10		coord: 118..309 score: 1.0
None	No IPR available	GENE3D	G3DSA:3.30.200.20		coord: 44..104 score: 5.6E-5coord: 8..43 score: 2.
None	No IPR available	PANTHER	PTHR24361	MITOGEN-ACTIVATED KINASE KINASE KINASE	coord: 1..309 score: 3.9
None	No IPR available	PANTHER	PTHR24361:SF303	SUBFAMILY NOT NAMED	coord: 1..309 score: 3.9