Lsi02G009750.1 (mRNA) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi02G009750.1 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.8e-149
Identity = 267/530 (50.38%), Postives = 357/530 (67.36%), Query Frame = 1

Query: 18  EEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 77
           E FL+C   H P+  +  K+VYT ++  Y SILN +I+NLRF +  TPKPL+I+TPSN S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 78  HIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQSVAWVQS 137
           HIQA ++CSK   LQIR RSGGHD EG+SY++  PF+V+DL N+ SI +D     AWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 138 GATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 197
           GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 198 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLE-DGTIK 257
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK+KLV VP+  TI + K+ +E  G +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 258 LINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPIA 317
           L N+WQ +A K D++L L    I                 IT   GK       K     
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFIT--------------KNITDNHGK------NKTTVHG 331

Query: 318 LFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLL 377
            F S+F G  D L+ ++ ++FPELG+ K DCKE SWI+++++ +    F + +   E LL
Sbjct: 332 YFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILL 391

Query: 378 NRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEI 437
           +R+     +F IK DYVK+PI + A+  I E+L  +D+G     L P+GG M +ISES I
Sbjct: 392 DRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAI 451

Query: 438 PFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDI 497
           PF HRAG +Y++ Y   WE+Q     ++++NW+R +Y + TP+VS++PR AY+NYRD D+
Sbjct: 452 PFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDL 511

Query: 498 GSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           G  N     +Y +AR WG KYFG  NFNRLV VKTKVDP NFFR+E SIP
Sbjct: 512 GKTNHASPNNYTQARIWGEKYFG-KNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of Lsi02G009750.1 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.1e-146
Identity = 265/537 (49.35%), Postives = 359/537 (66.85%), Query Frame = 1

Query: 11  TSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLI 70
           TS +   E FL+C   + P+  +  K+VYT NN  Y S+LN +I NLRF++  TPKPL+I
Sbjct: 25  TSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVI 84

Query: 71  ITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQ 130
           +TPS++SHIQ  ++CSK   LQIR RSGGHD EG+SY++  PF+++DL N+RSI +D   
Sbjct: 85  VTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHS 144

Query: 131 SVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNV 190
             AWV++GATLGE+YY + EK+  L+  AG CPTV  GGHF GGGYG L+R YGLAADN+
Sbjct: 145 QTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNI 204

Query: 191 IDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTL 250
           IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFG++VAWK++LV VP + T+ + K+ +
Sbjct: 205 IDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIM 264

Query: 251 E-DGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGG 310
           E    +KL+N+WQ +A K D++L L    I                           QG 
Sbjct: 265 EIHELVKLVNKWQNIAYKYDKDLLLMTHFIT--------------------RNITDNQGK 324

Query: 311 GKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGD 370
            K      F S+FLG  D L+ ++ ++FPELG+ K DC++ SWI++ ++ +    + + +
Sbjct: 325 NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDN 384

Query: 371 QALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMS 430
              E LL+R+   NG+FKIK DYVK+PI +     I E+L  +DIG    AL P+GG M 
Sbjct: 385 FNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMD 444

Query: 431 QISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYV 490
           +ISES IPF HRAG LY++ YI  WE+Q     +++LNWIR IY +MTP+VSK+PR AY+
Sbjct: 445 EISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYL 504

Query: 491 NYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           NYRD DIG N+     +Y +AR WG KYFG  NF+RLV VKT VDP NFFR+E SIP
Sbjct: 505 NYRDLDIGINDPKNPNNYTQARIWGEKYFG-KNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of Lsi02G009750.1 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.9e-145
Identity = 257/530 (48.49%), Postives = 354/530 (66.79%), Query Frame = 1

Query: 18  EEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 77
           E FL+C   + P+  +  K +YT ++  Y S+LN +I+NLRF++  TPKPL+I+TPSN+S
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 78  HIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQSVAWVQS 137
           HIQA+++CSK   LQIR RSGGHD EGLSY++  PF ++DL N+ ++ VD     AWV++
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 138 GATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 197
           GATLGE+YY I E +   +FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 198 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLE-DGTIK 257
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK+KLV VP+  TI + K+ +E  G +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 258 LINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPIA 317
           L N+WQ +A K D++L L                      IT   GK       K     
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHF--------------RTRNITDNHGK------NKTTVHG 331

Query: 318 LFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLL 377
            F S+FLG  D L+ ++ ++FPELG+ K DCKE SWI+++++ +    + + +   E LL
Sbjct: 332 YFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILL 391

Query: 378 NRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEI 437
           +R+     +F IK DYVK+ I + A+  I E+L  +++G     L P+GG M +ISES I
Sbjct: 392 DRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAI 451

Query: 438 PFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDI 497
           PF HRAG +Y++ Y   WE+Q     ++++NW+R +Y + TP+VS++PR AY+NYRD D+
Sbjct: 452 PFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDL 511

Query: 498 GSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           G  N     +Y +AR WG KYFG  NFNRLV VKTK DP NFFR+E SIP
Sbjct: 512 GKTNPESPNNYTQARIWGEKYFG-KNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of Lsi02G009750.1 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.7e-145
Identity = 260/530 (49.06%), Postives = 355/530 (66.98%), Query Frame = 1

Query: 18  EEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 77
           E FL+CL  + P   +  K+VYT ++  Y SILN +++NLRF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 78  HIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQSVAWVQS 137
           HIQ  ++CSK   LQIR RSGGHD EG+SY++  PF+++DL N+ S+ +D     AWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 138 GATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 197
           GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 198 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLE-DGTIK 257
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK++LV VP+  TI + K+ +E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 258 LINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPIA 317
           L+N+WQ +A   ++EL L       +  FI          IT        QG  K    +
Sbjct: 272 LVNKWQNIAYMYEKELLL-------FTHFITR-------NIT------DNQGKNKTTIHS 331

Query: 318 LFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLL 377
            F S+F G  D L+ ++ ++FPELG+ K DCK+ SWI++ ++ +    + + +   E LL
Sbjct: 332 YFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILL 391

Query: 378 NRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEI 437
           +R+     +F IK DYVK+PI + A+  I E+L  +D+G       P+GG M +ISES I
Sbjct: 392 DRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAI 451

Query: 438 PFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDI 497
           PF HRAG  Y+I YI  WE+Q     ++++NWIR +Y + TP+VS++PR AY+NYRD D+
Sbjct: 452 PFPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDL 511

Query: 498 GSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           G  N     +Y +AR WG KYFG  NFNRLV VKTKVDP NFFR+E SIP
Sbjct: 512 GKTNFESPNNYTQARIWGEKYFG-KNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Lsi02G009750.1 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.3e-144
Identity = 260/530 (49.06%), Postives = 355/530 (66.98%), Query Frame = 1

Query: 18  EEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNIS 77
           E FL+C   + P   +  K+VYT ++  Y SILN +I+NLRF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 78  HIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQSVAWVQS 137
           HIQ  ++CSK   LQIR RSGGHD EG+SY++  PF+++DL N+ S+ +D     AWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 138 GATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVD 197
           GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 198 ANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLE-DGTIK 257
            +G+  DR+SMGEDLFWAIRGGGG +FG++ AWK++LV VP+  TI + K+ +E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 258 LINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPIA 317
           L+N+WQ +A   ++EL L       +  FI          IT        QG  K    +
Sbjct: 272 LVNKWQNIAYMYEKELLL-------FTHFITR-------NIT------DNQGKNKTTIHS 331

Query: 318 LFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAAN--RFQSGDQALETLL 377
            F S+F G  D L+ ++ ++FPELG+ K DCK+ SWI++ ++ +    + +     E LL
Sbjct: 332 YFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILL 391

Query: 378 NRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEI 437
           +R+     +F IK DYVK+PI + A+  I E+L  +D+G       P+GG M +ISES I
Sbjct: 392 DRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAI 451

Query: 438 PFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDI 497
           PF HRAG +Y+I YI  WE+Q     ++++NWIR +Y + TP+VS++PR AY+NYRD D+
Sbjct: 452 PFPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDL 511

Query: 498 GSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           G  N     +Y +AR WG KYFG  NFNRLV VKTKVDP NFFR+E SIP
Sbjct: 512 GKTNFESPNNYTQARIWGEKYFG-KNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Lsi02G009750.1 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 3.9e-180
Identity = 324/542 (59.78%), Postives = 408/542 (75.28%), Query Frame = 1

Query: 5   SSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNT 64
           S SW  ++ +  HE FLQCL   S +++SI+K++YTP NSSYSSIL FSI+NLRF+T +T
Sbjct: 11  SFSWVTSAHT--HESFLQCL--DSQNSHSISKLIYTPINSSYSSILQFSIQNLRFNTSST 70

Query: 65  PKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSI 124
           PKPL+I+TP+NISHIQAA+ICS+ H +QIRIRSGGHD+EGLSYV+   F+V+DLINLR+I
Sbjct: 71  PKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDLINLRTI 130

Query: 125 AVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYG 184
            V+AE   AWVQSG T+GE+YYRIAEKSRTLAFPA +CPTVGVGGHFSGGGYGML+RKYG
Sbjct: 131 NVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGMLMRKYG 190

Query: 185 LAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTIC 244
           LAAD++IDA LVD  G   DRESMGEDLFWAIRGGGG +FGVV+AWK+ LV  P TVT+ 
Sbjct: 191 LAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAPPTVTVF 250

Query: 245 ATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKV 304
             +RTLE    KL+++WQ+VA+KL E+LF+ I L                        +V
Sbjct: 251 NVQRTLEQNASKLVHRWQFVADKLHEDLFIRIIL-----------------------NRV 310

Query: 305 SAQGGGKINPI--ALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 364
           ++   GK N    A F SLFLG  D L+ +++ +FPELGL KEDC E SWI+S LY  + 
Sbjct: 311 NSTEEGKSNTTIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYF-DG 370

Query: 365 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 424
           F S + +L+ LL+RT S   +FK KSDYVKEPI ++ +EGIWER   +DI    L   P+
Sbjct: 371 FPS-NSSLDVLLDRTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPY 430

Query: 425 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 484
           GG+MS+ISES IPF HRAGN+Y+I +++ W+E+GIVA KR+++WIR +Y+Y+ P+VSK+P
Sbjct: 431 GGKMSEISESSIPFPHRAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTP 490

Query: 485 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 544
           R+AYVNYRD DIG NN  G TSY++A  WG KYF  NNF+RLV VKT VDP NFFR+E S
Sbjct: 491 RAAYVNYRDLDIGINNHAGNTSYRQASIWGLKYF-KNNFDRLVRVKTAVDPANFFRNEQS 522

BLAST of Lsi02G009750.1 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 2.4e-177
Identity = 320/536 (59.70%), Postives = 390/536 (72.76%), Query Frame = 1

Query: 10  ATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLL 69
           A  +S   + FLQCL  +S +  SI+KVVYT  NSSYSSIL FSI+NLRF+T  TPKPL+
Sbjct: 23  AIDTSPHEDNFLQCLYSYSHNITSISKVVYTKTNSSYSSILKFSIQNLRFATNETPKPLV 82

Query: 70  IITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAE 129
           IITP+ ISHIQ A+ICS+ H +QIRIRSGGHDFEGLS+V+  PF+++DL N R I VD E
Sbjct: 83  IITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFVSNVPFVIIDLTNFRGIDVDVE 142

Query: 130 QSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADN 189
              AWVQSGATLGELYY+IA+KS+TL FP G+CPTVGVGGHFSGGGYG LLRKYGLAADN
Sbjct: 143 NRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVGGHFSGGGYGTLLRKYGLAADN 202

Query: 190 VIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRT 249
           VIDA+++D  G F DRE+MGEDLFWAIRGGGG SFGV+V+WK+KLV VP+TVT+    RT
Sbjct: 203 VIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIKLVQVPSTVTVFTVPRT 262

Query: 250 LEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGG 309
           LE    KL+++WQ+VA+KL+E L + I L                +++ S     S QG 
Sbjct: 263 LEQNATKLVHKWQFVAHKLEENLAINIIL--------------QRLDLNS-----SKQGE 322

Query: 310 GKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQA 369
            K   +ALF SLFLG  D L+ ++++ FPELGL +EDC E SWIES LY   RF  G + 
Sbjct: 323 PKSTVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLF-RFPEG-EP 382

Query: 370 LETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQI 429
           LETLLNRTL+A  + K KSD+VK PI +  +EG+W          V + L P+GG M +I
Sbjct: 383 LETLLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLFPYGGIMDKI 442

Query: 430 SESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNY 489
           SESEIPF HR G LY+I Y V W ++G   EK ++NWIR++Y+YM PFVSKSPR+AY+NY
Sbjct: 443 SESEIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINY 502

Query: 490 RDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 546
           RD DIG NN  G TSYK+A  WG KYF  NNF RL  VKTKVDP NFFR+E SIP+
Sbjct: 503 RDLDIGVNNINGYTSYKQASIWGVKYF-KNNFKRLAKVKTKVDPLNFFRNEQSIPS 536

BLAST of Lsi02G009750.1 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.1e-173
Identity = 311/537 (57.91%), Postives = 387/537 (72.07%), Query Frame = 1

Query: 8   WAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKP 67
           W+A++ +  HE+FLQCL  +S  + S +K+VYTPNNSSY  +L  SI+N RF+T  TPKP
Sbjct: 23  WSASAHT--HEDFLQCLSLYSEDSNSFSKLVYTPNNSSYLHVLQGSIQNSRFNTTATPKP 82

Query: 68  LLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVD 127
            +I+TP N+SHIQA + CS+ H + +RIRSGGHD+EGLSYV+  PF+++DLINLR+I VD
Sbjct: 83  PVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVSVLPFVIIDLINLRAINVD 142

Query: 128 AEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAA 187
            E S AWVQ+GATLGELYY IAEKS TLAFPAG CPTVG GGH SGGGYG L+RKYGLAA
Sbjct: 143 VENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGGHLSGGGYGGLMRKYGLAA 202

Query: 188 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATK 247
           DN+IDA L+DA G   DR SMGEDLFWAIRGGGG +FGVVVAWK+KLVPVP TVT  +  
Sbjct: 203 DNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWKLKLVPVPHTVTFFSVL 262

Query: 248 RTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQ 307
           R+LE+   KLI++WQYVANKL E+LF                       IT+   K+++ 
Sbjct: 263 RSLEENATKLIHRWQYVANKLPEDLF-----------------------ITAYITKINSS 322

Query: 308 GGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGD 367
             G     A F SLFLG AD L+ ++++ FPELGL K+DC E SW+E  LY  N   S +
Sbjct: 323 QEGISTIQAEFPSLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVLY--NSGYSSN 382

Query: 368 QALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMS 427
            +L+ LLNRT     +FK KSDYVK+P+ ++A EGIW+R     I    L L+P+GG+M 
Sbjct: 383 SSLDVLLNRTPQYITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMD 442

Query: 428 QISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYV 487
           QISES IPF+HRAGNLY+I Y++ W EQG  A  R++ WIR +Y+Y  P+VSK+PR AY+
Sbjct: 443 QISESSIPFAHRAGNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYI 502

Query: 488 NYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           NYRD D+G NN  G TSYK+A  WG KYF  NNF+RL+ VKT VDP NFFR+E SIP
Sbjct: 503 NYRDLDVGMNNIQGNTSYKQASIWGRKYF-KNNFDRLIQVKTAVDPDNFFRNEQSIP 531

BLAST of Lsi02G009750.1 vs. TrEMBL
Match: I1K2W7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_05G124800 PE=4 SV=2)

HSP 1 Score: 616.7 bits (1589), Expect = 2.7e-173
Identity = 310/540 (57.41%), Postives = 388/540 (71.85%), Query Frame = 1

Query: 6   SSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTP 65
           SS+ +++ +  +E F+QCL  +S ++ SI+KVVYT  N+SYSSIL+FSI+NLRFS+  TP
Sbjct: 32  SSFTSSAVNTNYENFIQCLYSYSHNSSSISKVVYTKTNASYSSILHFSIQNLRFSSNTTP 91

Query: 66  KPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIA 125
           KPL+I+TP+ +SHIQAA+ICS+ H LQIR RSGGHDFEGLSYVA  PF+V+DLIN R I 
Sbjct: 92  KPLVIVTPTEVSHIQAAIICSQRHGLQIRTRSGGHDFEGLSYVAEAPFVVIDLINYRRID 151

Query: 126 VDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGL 185
           VD  + VAWVQSGAT+GELYY I+EKS+TL FPAG+  TVGVGG F GGGYG LLRK+GL
Sbjct: 152 VDVNKRVAWVQSGATVGELYYSISEKSKTLGFPAGVFTTVGVGGQFGGGGYGFLLRKHGL 211

Query: 186 AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICA 245
           AADN++DAY+VDA G   DRE+M EDLFWAIRGGGG SFGV+VAWKVKLVPVP TVT+  
Sbjct: 212 AADNIVDAYIVDAKGRLLDREAMSEDLFWAIRGGGGASFGVIVAWKVKLVPVPPTVTVFR 271

Query: 246 TKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVS 305
             RTLE    KLI++WQ VA+KLD ++ + I +                        +V+
Sbjct: 272 VARTLEQNATKLIHKWQLVASKLDGDIAINILV-----------------------HRVN 331

Query: 306 AQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQS 365
           +   G+    ALF SL+LG  D+LM ++++ FPELGL +EDC E +WI+S LY       
Sbjct: 332 SSRKGEFTIEALFQSLYLGGLDKLMHLMQENFPELGLKREDCAEMTWIDSVLYFV----- 391

Query: 366 GDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGR 425
           G Q+ E LLNR+ +   SFK KSD+V+ PI +  +EG+W+ L      G  L L PFG  
Sbjct: 392 GYQSREALLNRSQTTTDSFKAKSDFVRNPIPEAGLEGLWQMLYEDGAQGALLVLFPFGAI 451

Query: 426 MSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSA 485
           M  I ESEIPF HR+GNLY + Y V W E+     +++++W+R +YTYM PFVSKSPR+A
Sbjct: 452 MDTIPESEIPFPHRSGNLYLVQYTVHWLEEEDEIAQKHISWVRRLYTYMEPFVSKSPRAA 511

Query: 486 YVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 545
           YVNYRD DIG NN  G TSYK+A  WGSKYF  NNFNRL HVKTKVDP NFFR+E SIP+
Sbjct: 512 YVNYRDLDIGVNNNIGYTSYKQASIWGSKYF-KNNFNRLAHVKTKVDPLNFFRYEQSIPS 542

BLAST of Lsi02G009750.1 vs. TrEMBL
Match: A0A0D2US10_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G121800 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 6.1e-173
Identity = 310/545 (56.88%), Postives = 393/545 (72.11%), Query Frame = 1

Query: 5   SSSWAAT--SSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 64
           S SWAA   +   +H  FLQCL  H  +  SI+ V+YT  NSS+SS+L FS+RN RFSTP
Sbjct: 22  SFSWAALGLTHQQQHHSFLQCLNRHFGNANSISTVIYTQTNSSFSSVLEFSLRNARFSTP 81

Query: 65  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 124
           NT KPL+I+T S++SHIQA + CS++H LQIRIRSGGHD+EGLSYV+  PF+++DLINLR
Sbjct: 82  NTLKPLVIVTASHVSHIQATINCSRTHGLQIRIRSGGHDYEGLSYVSQVPFVIIDLINLR 141

Query: 125 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 184
           SI VDAE   AW+Q+GAT+GELYYRIAEKS TLAFPAG+CPTVGVGGHFSGGGYGML+RK
Sbjct: 142 SIDVDAENKTAWIQAGATIGELYYRIAEKSSTLAFPAGVCPTVGVGGHFSGGGYGMLMRK 201

Query: 185 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 244
           YGLAAD +IDA LVD NG+  D+ SMGED+FWAIRGGG  SFGVVVAWKVKLVPVP+T+T
Sbjct: 202 YGLAADQIIDAQLVDVNGKLLDKNSMGEDMFWAIRGGGAASFGVVVAWKVKLVPVPSTLT 261

Query: 245 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 304
           +    RTLE+    L+++WQ +A K+ E+L++ +FL  +                     
Sbjct: 262 VFTVNRTLEENGTMLVHKWQSIAPKIHEDLYIRLFLRAVN-------------------- 321

Query: 305 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 364
             S+Q  GK    A F SL+LG+ADEL+ +++++FPELGL KEDC E SWI+S +Y  + 
Sbjct: 322 --SSQQEGKRTIQASFVSLYLGRADELIDLMQESFPELGLVKEDCIEMSWIQSIMYFPSD 381

Query: 365 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 424
               D  LE LLNRT SA G FK KSDYV + I + A+EG+W+R    +   + +   P+
Sbjct: 382 IPE-DAPLEILLNRTGSA-GIFKGKSDYVTQTIPETALEGLWQRFYEDETESLEILFSPY 441

Query: 425 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 484
           GG M  I E+E P+SHRAGNL+ I Y+VGW E+     +R +N++R +Y YM P+VSKSP
Sbjct: 442 GGNMDDIPETETPYSHRAGNLFNIHYVVGWSEEDASESQRYINFMRRLYRYMEPYVSKSP 501

Query: 485 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 544
           R AY+NYRD D+G+NN    TSYK+A KWG  YF   NFNRL+HVKT VDP NFFR++ S
Sbjct: 502 RRAYMNYRDLDLGTNNIGPYTSYKQASKWGLPYFD-KNFNRLIHVKTLVDPTNFFRYQQS 541

Query: 545 IPTPS 548
           IP+ S
Sbjct: 562 IPSLS 541

BLAST of Lsi02G009750.1 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 503.1 bits (1294), Expect = 2.2e-142
Identity = 266/540 (49.26%), Postives = 354/540 (65.56%), Query Frame = 1

Query: 8   WAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKP 67
           W ++SSS   E F QCL  +S   + I+  ++   N SYSS+L  +IRNLRF+T +TPKP
Sbjct: 16  WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKP 75

Query: 68  LLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYH--PFIVLDLINLRSIA 127
            LII  ++ SH+QAA+ C K H LQ++IRSGGHD++GLSYV Y   PF VLD+ NLRS+ 
Sbjct: 76  FLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVD 135

Query: 128 VDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGL 187
           VD     AWVQ+GA LGE+YY I EKS+TLA+PAGICPTVGVGGH SGGGYG ++RKYGL
Sbjct: 136 VDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGL 195

Query: 188 AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICA 247
             DN IDA +VD NG+  DR+ MGEDL+WAI GGGGGS+GVV+A+K+ LV VP  VT+  
Sbjct: 196 TVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFR 255

Query: 248 TKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVS 307
             RTLE     +I++WQ VA KL +ELF+   + V                   + G VS
Sbjct: 256 ISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDV-------------------VNGTVS 315

Query: 308 AQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQS 367
           +Q   +      F ++FLG    L++IL + FPELGL + DC ETSWI+S L+  N  Q 
Sbjct: 316 SQKTVR----TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQV 375

Query: 368 GDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGR 427
           G    ETLL +        K KSDYV+EPI++  +E IW+++   +I   T+A  P+GG 
Sbjct: 376 GSS--ETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWKKMIELEI--PTMAFNPYGGE 435

Query: 428 MSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSA 487
           M +IS +  PF +RAGNL++I Y   W ++ +    R +   R++Y +MTPFVSK+PR +
Sbjct: 436 MGRISSTVTPFPYRAGNLWKIQYGANWRDETLT--DRYMELTRKLYQFMTPFVSKNPRQS 495

Query: 488 YVNYRDRDIGSNNKFGK-TSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           + NYRD D+G N+  GK +SY   +++G KYF   NF RLV +KT+VD  NFFR+E SIP
Sbjct: 496 FFNYRDVDLGINSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Lsi02G009750.1 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 496.5 bits (1277), Expect = 2.1e-140
Identity = 255/532 (47.93%), Postives = 352/532 (66.17%), Query Frame = 1

Query: 17  HEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNI 76
           HE+FL+CL +         KV++T  +SS+ SIL+ SI+N RFS   TPKP+ IITP   
Sbjct: 28  HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 87

Query: 77  SHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYH-PFIVLDLINLRSIAVDAEQSVAWV 136
           S +Q  + C++ H + +R RS GH +EGLSY+AY+ PF V+DL NLRSI++D +    WV
Sbjct: 88  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 147

Query: 137 QSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYL 196
           Q+GAT GELYY I + +++LAFPAGI PTVGVGG FSGGGYG LLRKYGLAADN+IDA +
Sbjct: 148 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 207

Query: 197 VDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLEDGTI 256
           VDA+G   DR++MGED FWAIRGGGG SFGV+++WKVKLV VP+T+T+   ++T +   +
Sbjct: 208 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 267

Query: 257 KLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPI 316
           ++I +WQY A+K+ ++LF+                            + + +   K    
Sbjct: 268 RIIKKWQYAADKVPDDLFI----------------------------RTTLERSNKNAVH 327

Query: 317 ALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLN 376
           ALF  L++G  + L+ ++++ FPELGL KE C+E SWIES L+ A+ F  G+ +L  L N
Sbjct: 328 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGE-SLGVLTN 387

Query: 377 RTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIP 436
           R  ++  SFK K D+V+EPI + AI+ IW RL++ +     + L PFGG+MS+++E E P
Sbjct: 388 RERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETP 447

Query: 437 FSHRAGNLYQIGYIVGW---EEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDR 496
           F HR GNLY+I Y+  W   E++      + L W+  +Y +MTP+VSKSPR AYVN++D 
Sbjct: 448 FPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDM 507

Query: 497 DIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           D+G      KT Y+  + WG KYF  NNF RLV VKT+VDP +FF  E SIP
Sbjct: 508 DLGMYLGKKKTKYEEGKSWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Lsi02G009750.1 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 495.0 bits (1273), Expect = 6.1e-140
Identity = 254/538 (47.21%), Postives = 362/538 (67.29%), Query Frame = 1

Query: 12  SSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSS--YSSILNFSIRNLRFSTPNTPKPLL 71
           SSS   ++F++CL+ +S  ++ IT   ++P+ ++  +   L  + +NLR+ TP+ PKP+ 
Sbjct: 25  SSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVF 84

Query: 72  IITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYH--PFIVLDLINLRSIAVD 131
           I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+VA    PF+++DL  LR + VD
Sbjct: 85  IFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVD 144

Query: 132 AEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAA 191
            + + AW  +GAT+GE+YYRI EKS+T  FPAG+C ++G+GGH  GG YG ++RK+GL A
Sbjct: 145 LDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGA 204

Query: 192 DNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATK 251
           DNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFGV++AWK+KLVPVPATVT+    
Sbjct: 205 DNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVT 264

Query: 252 RTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQ 311
           +TLE    K++ +W+ +A+KLD++LF+ +                    I S   K +  
Sbjct: 265 KTLEQDGTKVLYKWEQIADKLDDDLFIRV--------------------IISPASKTTKP 324

Query: 312 GGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGD 371
           G   I+    + + FLG ++ L+ +++++FPELGLTK+DC E SWI+S +Y A    S  
Sbjct: 325 GNRTIS--MSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSA- 384

Query: 372 QALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMS 431
            A E LL         FK KSD+VKEPI    +EG+WER   +D         P+GG MS
Sbjct: 385 -APEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEED--SPLTIWNPYGGMMS 444

Query: 432 QISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYV 491
           +ISESEIPF HR G L++I ++  W++ G V+E+R++ WIRE+Y+YM  +VSK+PR AYV
Sbjct: 445 RISESEIPFPHRNGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYV 504

Query: 492 NYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIPT 546
           NYRD D+G+N   G+T    AR+WG+KY+   NF RLV +K + DP NFFRHE S+PT
Sbjct: 505 NYRDLDLGTNE--GETD---AREWGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Lsi02G009750.1 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 491.1 bits (1263), Expect = 8.8e-139
Identity = 254/525 (48.38%), Postives = 347/525 (66.10%), Query Frame = 1

Query: 20  FLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSNISHI 79
           FLQCL      +  ++KV++TPN++S+SS+L  SI+N RFS P+ PKP+LI+TP   S +
Sbjct: 37  FLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDV 96

Query: 80  QAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLRSIAVDAEQSVAWVQSGA 139
           Q+AV C++   + IR RSGGHD+EGLSYV + PF++LDL NLRSI VD +    WVQ+GA
Sbjct: 97  QSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGA 156

Query: 140 TLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDAYLVDAN 199
           T+GELYY I +K+RTLAFPAG+CPTVGVGGHFSGGGYG LLRK+GLAAD+VIDA +VDA 
Sbjct: 157 TIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDAR 216

Query: 200 GEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLEDGTIKLIN 259
           G   +R  MGED FWAIRGGGG SF VV++WK+ L+ VP+TVT+    +  E   +K+I+
Sbjct: 217 GRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIH 276

Query: 260 QWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKINPIALFF 319
           +WQ+VA+K+ ++LF+ + L   Y + +                             A F 
Sbjct: 277 RWQFVADKVSDDLFIRVML-QRYKNMVR----------------------------ASFP 336

Query: 320 SLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALETLLNRTLS 379
            L+LG    L+ ++ + FPELGL ++DC E SWIES ++ A   + G++ +  L  RT  
Sbjct: 337 GLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRT-R 396

Query: 380 ANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISESEIPFSHR 439
           A+ +FK KSD+V+EP+ K AI  +W RL+  +     L   PFGG+MS+I++ E PF HR
Sbjct: 397 ASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHR 456

Query: 440 AGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDRDIGSNNK 499
            GN+Y+I Y+  W  +G V EK  + W+  +Y  M+ FV+KSPR AY+N RD D+G    
Sbjct: 457 KGNIYEIQYLNYW--RGDVKEK-YMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVG 516

Query: 500 FGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
             ++ Y+  + WG KYF  NNF RLV VKT VDP +FF  E SIP
Sbjct: 517 VKRSKYEEGKSWGVKYF-KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Lsi02G009750.1 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 484.6 bits (1246), Expect = 8.2e-137
Identity = 249/532 (46.80%), Postives = 350/532 (65.79%), Query Frame = 1

Query: 18  EEFLQCLLHHSPHTYSITKVVYTP--NNSSYSSILNFSIRNLRFSTPNTPKPLLIITPSN 77
           ++F+ C+  ++   + + K  + P  N S +S +L  + +NLRF   + PKP  I +P +
Sbjct: 33  DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92

Query: 78  ISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAY--HPFIVLDLINLRSIAVDAEQSVA 137
            SH+QA++ICSK   + +R+RSGGHD+EGLSYV+    PFI++DL  +R + ++ + + A
Sbjct: 93  ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152

Query: 138 WVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRKYGLAADNVIDA 197
           WVQSGAT+GELYYRIAEKS+   FPAG+C ++G+GGH +GG YG ++RKYGL ADNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212

Query: 198 YLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVTICATKRTLE-D 257
            +VDANG+  DR +MGED FWAIRGG GGSFG+++AWK+KLVPVP TVT+    +TL+ D
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272

Query: 258 GTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGGKVSAQGGGKI 317
              K+I++WQ VA+KL EELF+ +   V                         A  GG  
Sbjct: 273 VGNKIISKWQRVADKLVEELFIRVLFNV-------------------------AGTGGNK 332

Query: 318 NPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANRFQSGDQALET 377
                + +LFLG    LM ++K++FPELGLT +DC E SW+ES  Y +  F +       
Sbjct: 333 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG-FPTHTPTNVL 392

Query: 378 LLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPFGGRMSQISES 437
           L  ++     SFK KSD+VK PI +  ++GI+++L  +DI    +   P+GG M++I ES
Sbjct: 393 LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMIWNPYGGMMAKIPES 452

Query: 438 EIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSPRSAYVNYRDR 497
           +IPF HR G L+++ Y+  W +       R++NWIR++Y+YMTP+VS +PR AYVNYRD 
Sbjct: 453 QIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDL 512

Query: 498 DIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELSIP 545
           D+G N K  KT  K+A+ WG+ YF  NNFNRL+ +K KVDP NFFRHE SIP
Sbjct: 513 DLGRNTKDVKTCIKQAQVWGANYF-KNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of Lsi02G009750.1 vs. NCBI nr
Match: gi|778662006|ref|XP_011659211.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 849.4 bits (2193), Expect = 3.6e-243
Identity = 423/545 (77.61%), Postives = 469/545 (86.06%), Query Frame = 1

Query: 3   SSSSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 62
           SSSS W A SSS+ HEEF+QCLLHHSP TY ITK+V+TP NSS+SS+LNF  RN RFSTP
Sbjct: 18  SSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTP 77

Query: 63  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 122
           NTPKPLLIITPSNISHIQAAV+CSKSH LQIRIRSGGHDFEGLSYVAYH FIV+DLINL+
Sbjct: 78  NTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLK 137

Query: 123 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 182
           S+ VD EQS AWV+SGATLGELYY+I +KSRTL FPAGICPTVGVGGHFSGGGYG LLRK
Sbjct: 138 SVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRK 197

Query: 183 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 242
           YG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVPATVT
Sbjct: 198 YGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT 257

Query: 243 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 302
           IC+ KR+LE+  IKLI+QWQYVANKL+EELFL IF                       GG
Sbjct: 258 ICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFW---------------------NGG 317

Query: 303 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 362
           KVSAQ GGKINPIALFF+LFLG  +ELM IL +TFPELGLTKE+CKE SWIES+  AANR
Sbjct: 318 KVSAQEGGKINPIALFFTLFLGNVNELMAILSKTFPELGLTKEECKEKSWIESAASAANR 377

Query: 363 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 422
           FQ  DQ LE LLNRT   +GSFK+KSDYVKEP+TK AI+GIW+RL+SQDI GVTLA+IP+
Sbjct: 378 FQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPY 437

Query: 423 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 482
           GGRM+QISES+IPF HRAG LYQIGYI+GWEE+G+ AEKR+LNWIREIY+YMTPFVSKSP
Sbjct: 438 GGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSP 497

Query: 483 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 542
           R+AYVNYRD DIGSNNK+GK +YK+A  +GSKYFG NNFNRLV VK+ VDPYNFF HE S
Sbjct: 498 RAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFG-NNFNRLVKVKSDVDPYNFFWHEQS 540

Query: 543 IPTPS 548
           IP  S
Sbjct: 558 IPLSS 540

BLAST of Lsi02G009750.1 vs. NCBI nr
Match: gi|659068694|ref|XP_008445721.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 845.1 bits (2182), Expect = 6.8e-242
Identity = 425/546 (77.84%), Postives = 469/546 (85.90%), Query Frame = 1

Query: 3   SSSSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 62
           SSSS WAATSSS+KHEEFLQCLLHHSP T SI K+V+TP NSS+S++ NF  RN RFSTP
Sbjct: 31  SSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFSTLFNFPTRNSRFSTP 90

Query: 63  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 122
           NTPKPLLIITPSNISHIQAAVICSKSH LQIRIRSGGHDFEGLSYVAYH FIV+DLINLR
Sbjct: 91  NTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHRFIVVDLINLR 150

Query: 123 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 182
           S+ VD EQS AWVQSGATLGELYY+I +KSRTL FPAGICPTVGVGGHFSGGGYG LLRK
Sbjct: 151 SVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRK 210

Query: 183 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 242
           YG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPA+VT
Sbjct: 211 YGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPASVT 270

Query: 243 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 302
           IC+ KR++E+  IKLI++WQYVANKL+E+LFL I                        GG
Sbjct: 271 ICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILW---------------------NGG 330

Query: 303 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 362
           KVSAQGGGKINPIALFF LFLG  +ELM IL +TFPELG TKE+CKE SWIES+  AANR
Sbjct: 331 KVSAQGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANR 390

Query: 363 FQSGDQALETLLNRT-LSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIP 422
           FQ  DQ LE LLNRT ++   SFK+KSDYVKEP+TK AIEGIW+RL+SQDI GVTLALIP
Sbjct: 391 FQIEDQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIP 450

Query: 423 FGGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKS 482
           +GGRMS+ISES IPFSHRAG LYQIGYI+GW+E+G+ AE+R+LNWIREIY+YMTPFVSKS
Sbjct: 451 YGGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKS 510

Query: 483 PRSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHEL 542
           PR+AYVNYRD DIGSNNK+GK +YK+A  +GSKYFG NNFNRLV VKTKVDPYNFF HE 
Sbjct: 511 PRTAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFG-NNFNRLVKVKTKVDPYNFFWHEQ 554

Query: 543 SIPTPS 548
           SIP  S
Sbjct: 571 SIPLSS 554

BLAST of Lsi02G009750.1 vs. NCBI nr
Match: gi|659068692|ref|XP_008445709.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 839.0 bits (2166), Expect = 4.9e-240
Identity = 415/542 (76.57%), Postives = 462/542 (85.24%), Query Frame = 1

Query: 3   SSSSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 62
           SSSS W + SS + HEEFL CLLHHSP + SITK+VYTP N+SYS +LNFSIRNLRFSTP
Sbjct: 18  SSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIRNLRFSTP 77

Query: 63  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 122
           NTPKPLLIITPSNISH+QAAVICSKSH +QIRIRSGGHDFEGLSYVAYHPF+V+DLINL 
Sbjct: 78  NTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVVVDLINLS 137

Query: 123 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 182
           S+ ++ EQS AWVQSGATLGELYYRIAEKSRTLAFPAG CPTVGVGGHFSGGG+G LLRK
Sbjct: 138 SVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGGFGTLLRK 197

Query: 183 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 242
           YGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVPATVT
Sbjct: 198 YGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT 257

Query: 243 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 302
           IC   RTLE+  IKLI+QWQYVANKLDE+LFLGI L                     +GG
Sbjct: 258 ICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINL---------------------LGG 317

Query: 303 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 362
           K+ AQ G KINPIALFFSLFLGKADELM IL +TFP+LGLTKE+CKETSWIES +Y  N 
Sbjct: 318 KIVAQ-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNG 377

Query: 363 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 422
            Q  DQ LE LLNRT  ANG+ K+KSDYVKEPI+K  I+ +W+RLKSQDI G  L  IP+
Sbjct: 378 LQIEDQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPY 437

Query: 423 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 482
           GGRMSQISESEIPFSHRAGNLY+IGY+ GW E G+ AEK +LNWIR+IY+YMTPFV+KSP
Sbjct: 438 GGRMSQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSP 497

Query: 483 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 542
           R+AYVNYRD DIGSN+K+GKTSYKRA  WGSKYFG +NF+RLV+VK +VDPYNFFRHE S
Sbjct: 498 RAAYVNYRDLDIGSNSKYGKTSYKRAHVWGSKYFG-SNFDRLVYVKNEVDPYNFFRHEQS 536

Query: 543 IP 545
           IP
Sbjct: 558 IP 536

BLAST of Lsi02G009750.1 vs. NCBI nr
Match: gi|778662003|ref|XP_011659204.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 830.1 bits (2143), Expect = 2.3e-237
Identity = 411/542 (75.83%), Postives = 457/542 (84.32%), Query Frame = 1

Query: 3   SSSSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 62
           SSSS W + SS + HEEFLQCLLHHSPH+ SI K+ YTP N+SYSS+LNFSIRNLRFS P
Sbjct: 18  SSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIRNLRFSIP 77

Query: 63  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 122
           NTPKPL IITP+NISH+QAAVICSKSH LQIRIRSGGHDFEGLSYVAYHPFI++DLI+L 
Sbjct: 78  NTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLS 137

Query: 123 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 182
           S+ ++ +QS AWVQSGATLGELYYRIAEKSRTLAFPAG  PTVGVGGHFSGGG+G LLRK
Sbjct: 138 SVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRK 197

Query: 183 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 242
           YGLAADNVIDAYLVDA+G FHDR+SMGEDLFWAIRGGGGGSFG+VVAWKVKLVPVPATVT
Sbjct: 198 YGLAADNVIDAYLVDAHGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVT 257

Query: 243 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 302
           IC   RTLE+  IKL++QWQYVANKLDE+LFLGI L                     +GG
Sbjct: 258 ICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINL---------------------LGG 317

Query: 303 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 362
           K+SAQ G KINPIALFFSLFLGKADELM IL +TFP+LGLTKE+CKETSWIES +Y  N 
Sbjct: 318 KISAQ-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNG 377

Query: 363 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 422
            Q  DQ LE LLNRT  A G+ K+KSDYVKEPI K  IE IW+RL+SQDI G  L  +P+
Sbjct: 378 LQIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPY 437

Query: 423 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 482
           GGRMSQIS+SEIPFSHRAGNLY+IGY+ GW E G+ AEK +LNWIR+IY YMTPFVSKSP
Sbjct: 438 GGRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSP 497

Query: 483 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 542
           R+AYVNYRD DIGSN+K+GKTSYKRAR WG KYFG NNFNR+V+VK KVDPYNFFRHE S
Sbjct: 498 RAAYVNYRDLDIGSNSKYGKTSYKRARVWGLKYFG-NNFNRMVYVKNKVDPYNFFRHEQS 536

Query: 543 IP 545
           IP
Sbjct: 558 IP 536

BLAST of Lsi02G009750.1 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 753.8 bits (1945), Expect = 2.1e-214
Identity = 381/543 (70.17%), Postives = 434/543 (79.93%), Query Frame = 1

Query: 3   SSSSSWAATSSSLKHEEFLQCLLHHSPHTYSITKVVYTPNNSSYSSILNFSIRNLRFSTP 62
           SSSS W + S   KH  FLQCL  HS  +Y I+KVV+TP NSSYSS+LNFSIRNLRFS P
Sbjct: 18  SSSSLWVSASPH-KHHNFLQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIRNLRFSKP 77

Query: 63  NTPKPLLIITPSNISHIQAAVICSKSHALQIRIRSGGHDFEGLSYVAYHPFIVLDLINLR 122
            TPKPLLIITPS++SHI+AAVICSKSH LQIR RSGGHD+EGLSYVA HPFI+LDLINLR
Sbjct: 78  ETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYVASHPFIILDLINLR 137

Query: 123 SIAVDAEQSVAWVQSGATLGELYYRIAEKSRTLAFPAGICPTVGVGGHFSGGGYGMLLRK 182
           S+ +D E + AWV+SG TLGELYYRI EKSRTLAFPAGICPTVGVGGHFSGGGYG++LRK
Sbjct: 138 SMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGLMLRK 197

Query: 183 YGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVVVAWKVKLVPVPATVT 242
           +GLAADNVIDAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFG+VVAWK+KLV VPATVT
Sbjct: 198 FGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPATVT 257

Query: 243 ICATKRTLEDGTIKLINQWQYVANKLDEELFLGIFLIVIYCSFILLLGIDALVEITSIGG 302
           +C T R L+   IKL++QWQYVANKLDE LFLGI L                      GG
Sbjct: 258 MCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIIL---------------------TGG 317

Query: 303 KVSAQGGGKINPIALFFSLFLGKADELMTILKQTFPELGLTKEDCKETSWIESSLYAANR 362
            ++    G  NPIA FFSLFLGK +EL+  L  TFPELGL K+DC ETSWIES+L A+  
Sbjct: 318 NITTTQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTG 377

Query: 363 FQSGDQALETLLNRTLSANGSFKIKSDYVKEPITKVAIEGIWERLKSQDIGGVTLALIPF 422
            Q+  ++LE LLNRT S   + KIKSDY+KEPI+   IEGIW+RLK+QDI    L  IP+
Sbjct: 378 VQT-VESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPY 437

Query: 423 GGRMSQISESEIPFSHRAGNLYQIGYIVGWEEQGIVAEKRNLNWIREIYTYMTPFVSKSP 482
           GGRMSQISESE PFSHR GNLY+IGYI+GW+EQ + A+K++++WIREIY YMTPFVSKSP
Sbjct: 438 GGRMSQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSP 497

Query: 483 RSAYVNYRDRDIGSNNKFGKTSYKRARKWGSKYFGYNNFNRLVHVKTKVDPYNFFRHELS 542
           RSAY NYRD DIG N K+GKTS K+A  WG KYFG NNF RLV+VKTKVDPY+FFRHE S
Sbjct: 498 RSAYANYRDLDIGVNKKYGKTSVKQASIWGLKYFG-NNFKRLVYVKTKVDPYDFFRHEQS 536

Query: 543 IPT 546
           IPT
Sbjct: 558 IPT 536