Cucsa.372010.2 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.372010.2 vs. Swiss-Prot
Match: AGD4_ARATH (ADP-ribosylation factor GTPase-activating protein AGD4 OS=Arabidopsis thaliana GN=AGD4 PE=2 SV=2)

HSP 1 Score: 879.0 bits (2270), Expect = 3.7e-254
Identity = 457/779 (58.66%), Postives = 561/779 (72.02%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M  FI LEDSPMF KQ+ SL+ T D L+DRC++L+ G + FM TLGEAS G+ +FA  LE
Sbjct: 1   MATFINLEDSPMFQKQVCSLEGTADELKDRCQKLYKGVKKFMGTLGEASKGESAFAACLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGG DDP+S+SIGGPV+SKF+ A RELA+YKE L SQVEHVL  RLM F+  DLQ  K
Sbjct: 61  EFGGGPDDPISLSIGGPVISKFINALRELASYKEFLCSQVEHVLLERLMNFISVDLQEAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR RFDKA H YDQ+REKFVSLKKNTRG+IVAE EEDL NSKS FEKSRFNLVN+LM +
Sbjct: 121 ESRHRFDKAAHSYDQSREKFVSLKKNTRGEIVAELEEDLENSKSTFEKSRFNLVNSLMTI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MDAHLRYFKLG+DL  Q+EPFIHQ+LTYAQQSKE + IEQD+LA+
Sbjct: 181 EAKKKYEFLESISAIMDAHLRYFKLGYDLLNQLEPFIHQILTYAQQSKEQSKIEQDRLAR 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQ++LD+ Q   + ++S  +    G  I  Y++ ET+    +    E IKQGYL
Sbjct: 241 RIQEFRTQSELDSQQLVANAESSGANGNRVGGNI-PYKNTETS----LTADKEVIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDE------------- 360
           LKRSS  R DWKR FFVLDS G++YYY+   ++ +G   H    SD              
Sbjct: 301 LKRSSSLRTDWKRKFFVLDSHGSMYYYRTNGNKSMGSHHHYSGSSDHNTGVFGRFRARHN 360

Query: 361 -----------HNNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINK 420
                      +N I+L TS IK+DAED DLRLCFRIISP KT+TLQAEN ADRMDW+NK
Sbjct: 361 RSGSLTEGSLGYNTIDLRTSLIKLDAEDMDLRLCFRIISPQKTYTLQAENGADRMDWVNK 420

Query: 421 ITGAIASLFNSYFLQQP---FLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKIL 480
           IT AI +L NS+FLQQ    +L+  +      N      Q RH    ++    D VS IL
Sbjct: 421 ITKAIGTLLNSHFLQQSPVRYLDKDNSSSAPANAVVSGDQIRHNDSRQNIG--DDVSTIL 480

Query: 481 REIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSI 540
           R +PGN+ CAEC + EP+WASLNLG+LLCI+CSGVHRNLGVHISKVRS+ LDVKVWEP+I
Sbjct: 481 RGLPGNNACAECNAPEPDWASLNLGVLLCIQCSGVHRNLGVHISKVRSLSLDVKVWEPTI 540

Query: 541 LDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEK 600
           LDLFRNLGN YCNS+WEGL   D   ++       S+ KP P+D+  ++E+YI  KY+EK
Sbjct: 541 LDLFRNLGNVYCNSLWEGLLHLDDDCEDGSALSHASVSKPCPEDSFSVKEKYILGKYLEK 600

Query: 601 QFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSA-VSIVNTTYDNVVSVSSSPHL 660
             V+KD E+  N+S A  IWEAV++ ++RE YRLI  +  V+I+NT +D++  + +  H+
Sbjct: 601 ALVIKD-ESEANLSAASRIWEAVQSRNIREIYRLIVTTGDVNIINTKFDDITDIDAYHHI 660

Query: 661 DEEPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVC 720
           D      ++  +P+ C R  +SNE    LQGCSLLH+AC   + V+LELLLQFG D+N+ 
Sbjct: 661 DAAEKAVKKRHDPTVCQRIKESNEPRSCLQGCSLLHVACHIGDSVLLELLLQFGADLNIR 720

Query: 721 DSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLL 752
           D HGRTPLH CIS  N+K AK+LLRRGA+PSI+D GGLS LERAMEMGAITDEELFLLL
Sbjct: 721 DYHGRTPLHHCISSGNHKFAKILLRRGARPSIEDDGGLSVLERAMEMGAITDEELFLLL 771

BLAST of Cucsa.372010.2 vs. Swiss-Prot
Match: AGD2_ARATH (ADP-ribosylation factor GTPase-activating protein AGD2 OS=Arabidopsis thaliana GN=AGD2 PE=2 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 2.3e-248
Identity = 461/783 (58.88%), Postives = 563/783 (71.90%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M  FI LEDSPMF KQ+ SL+ T+D L+DRC++L+ G + FM  LGEAS G  +FADSLE
Sbjct: 1   MAGFINLEDSPMFQKQVFSLEGTSDELKDRCQKLYKGVKKFMGALGEASTGVSAFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FG G DDPVSVSIGGPV+SKF+   REL++YKE LRSQVEHVL  RL  F+  DLQ  K
Sbjct: 61  EFGAGHDDPVSVSIGGPVISKFINTLRELSSYKEFLRSQVEHVLLERLTNFMTVDLQEAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+RFDKA+H YDQAREKFVSLKKNTRGDIVAE EEDL NSKSAFEKSRFNLVN+LM +
Sbjct: 121 ESRRRFDKAVHSYDQAREKFVSLKKNTRGDIVAELEEDLENSKSAFEKSRFNLVNSLMTI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MD+H +YFKLG+DL  Q+EP+IHQVLTYAQQSKE + IEQD+ A+
Sbjct: 181 EAKKKYEFLESISAIMDSHFKYFKLGYDLLSQLEPYIHQVLTYAQQSKEQSKIEQDRFAQ 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSV---ETAPQSIVNGKVETIKQ 300
           RIQEFRTQ++LD+ QAS   D S V       G + YR++        S+     E  KQ
Sbjct: 241 RIQEFRTQSELDSQQASAKADPSDV------GGNHVYRAIPRKNVEANSVSTADKEVTKQ 300

Query: 301 GYLLKRSSGSRGDWKRMFFVLDSQGALYYYKNKASR-------YVGQQSHS--------- 360
           GYLLKRS+  R DWKR FFVLD+ G+LYYY+N  ++       Y G   HS         
Sbjct: 301 GYLLKRSASLRADWKRRFFVLDNHGSLYYYRNTGNKSAKSQHYYSGLGEHSSGVFGRFRT 360

Query: 361 -FNRSDEH-----NNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
             NRS        N I+L TS IK+DAED+DLRLCFRIISP KT+TLQAEN ADRMDW+N
Sbjct: 361 RHNRSASQGSLDCNMIDLRTSLIKLDAEDTDLRLCFRIISPQKTYTLQAENGADRMDWVN 420

Query: 421 KITGAIASLFNSYFLQQP---FLEAKHPECDKNNFN-DYSVQTRHEKRLEDGSQVDSVSK 480
           KIT AI    NS+FLQQ    +L+ K+        N   + +  + +RL  G   D V  
Sbjct: 421 KITAAITIRLNSHFLQQSPARYLDKKNTSSGPATENLTLNQKEDYNQRLNVG---DDVLT 480

Query: 481 ILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEP 540
           ILREIPGN+ CAEC + +P+WASLNLG+L+CIECSGVHRNLGVHISKVRS+ LDVKVWEP
Sbjct: 481 ILREIPGNNTCAECNAPDPDWASLNLGVLMCIECSGVHRNLGVHISKVRSLTLDVKVWEP 540

Query: 541 SILDLFRNLGNTYCNSIWEGLF--LADTGRDNTQHALATSIPKPSPKDANQLRERYIQAK 600
           +ILDLFRNLGN YCNS+WE L   L D     +   LA S+ KPS +D   L+E+YI  K
Sbjct: 541 TILDLFRNLGNGYCNSVWEELLHHLDDDSEKGSTDTLA-SVSKPSSEDWFTLKEKYINGK 600

Query: 601 YVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSS 660
           Y+EK  VVKD E   N + +  IWEAV++ ++R+ YRLI  +  +I+NT +D++  +   
Sbjct: 601 YLEKALVVKD-EREANSTASSRIWEAVQSRNIRDIYRLIVKADANIINTKFDDITDLDVY 660

Query: 661 PHLD-EEPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTD 720
            H   + P   ++  +P++C R  +SNE+ + LQGCSLLH+ACQ+ + ++LELLLQFG D
Sbjct: 661 HHHHVDAPDEVKKRHDPNACQRIKNSNEARNCLQGCSLLHVACQSGDPILLELLLQFGAD 720

Query: 721 INVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELF 752
           IN+ D HGRTPLH CI+  NN  AK+LLRRGA+PSI+D GGLS LERAMEMGAITDEELF
Sbjct: 721 INMRDYHGRTPLHHCIASGNNAFAKVLLRRGARPSIEDGGGLSVLERAMEMGAITDEELF 772

BLAST of Cucsa.372010.2 vs. Swiss-Prot
Match: AGD3_ARATH (ADP-ribosylation factor GTPase-activating protein AGD3 OS=Arabidopsis thaliana GN=AGD3 PE=1 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 1.2e-191
Identity = 375/817 (45.90%), Postives = 507/817 (62.06%), Query Frame = 1

Query: 4   FIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLEGFG 63
           F KL+DSPMF KQ+ S++ + ++L +R  + + GCR + E LGEA +GDI+FA +LE FG
Sbjct: 3   FTKLDDSPMFRKQLQSMEESAEILRERSLKFYKGCRKYTEGLGEAYDGDIAFASALETFG 62

Query: 64  GGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSR 123
           GG +DP+SV+ GGPV++KF  A RE+ TYKE LRSQVEH+L +RL+QF + DL  VK++R
Sbjct: 63  GGHNDPISVAFGGPVMTKFTIALREIGTYKEVLRSQVEHILNDRLLQFANMDLHEVKEAR 122

Query: 124 QRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAK 183
           +RFDKA   YDQAREKF+SL+K T+ D+ A  E++L  S+S FE++RFNLV AL NVEAK
Sbjct: 123 KRFDKASLTYDQAREKFLSLRKGTKSDVAAALEQELHTSRSMFEQARFNLVTALSNVEAK 182

Query: 184 KKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQ 243
           K++EFLE++S  MDAHLRYFK G++L  QMEP+I+QVLTYAQQS+E +N EQ  L +++Q
Sbjct: 183 KRFEFLEAVSGTMDAHLRYFKQGYELLHQMEPYINQVLTYAQQSRERSNYEQAALNEKMQ 242

Query: 244 EFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKR 303
           E++ Q   ++   SN  + S   DGI   G +S++ ++   QS   GKV+TI+QGYL KR
Sbjct: 243 EYKRQVDRESRWGSNGSNGSPNGDGIQAIGRSSHKMIDAVMQSAARGKVQTIRQGYLSKR 302

Query: 304 SSGSRGDWKRMFFVLDSQGALYYYKNKA------------------------SRYVGQQS 363
           SS  RGDWKR FFVLDS+G LYYY+ +                         SR++   +
Sbjct: 303 SSNLRGDWKRRFFVLDSRGMLYYYRKQCSKPSGSGSQLSGQRNSSELGSGLLSRWLSSNN 362

Query: 364 HSF-----NRSDEHNNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWI 423
           H        +S   + +NL TSTIK+DA+ SDLR CFRIISP+K +TLQAE+  D+MDWI
Sbjct: 363 HGHGGVHDEKSVARHTVNLLTSTIKVDADQSDLRFCFRIISPTKNYTLQAESALDQMDWI 422

Query: 424 NKITGAIASLFNSYFLQQ-----PFLEAKH---PECDKNNFNDYSVQTRHE--------- 483
            KITG IASL +S   +Q     P     H    E      ++Y   T  E         
Sbjct: 423 EKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCERSFLG 482

Query: 484 ------KRLEDGSQVDSVSK---ILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGV 543
                 +  +    +    K    LR++ GND CA+CG+ EP+WASLNLG+L+CIECSGV
Sbjct: 483 YNERPSRSFQPQRSIRKGEKPIDALRKVCGNDKCADCGAPEPDWASLNLGVLVCIECSGV 542

Query: 544 HRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWEGLFLA------DTGRDNT 603
           HRNLGVHISKVRS+ LDVKVWEPS++ LF+ LGNT+ N++WE L  +      D G   +
Sbjct: 543 HRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIHFDPGLTVS 602

Query: 604 QHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLR 663
             +      KPS  D   ++E+YIQAKY EK FV +  ++    S A+ +W+AV  ND +
Sbjct: 603 DKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWDAVSGNDKK 662

Query: 664 EAYRLIAVSAVSIVNTTYDNVVSVSSSPH----LDEEPSGNQESLNPSSCTRD---WDSN 723
             YRLI V+  + VN  YD   S S +      + E P      L   +   D     SN
Sbjct: 663 AVYRLI-VNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELLDRTGSSSN 722

Query: 724 ESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNKLAKLL 753
            S +   G SLLH AC+  +  M+ELLLQ+G ++N  DS G+TPLH C+ +    +A+LL
Sbjct: 723 ISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRGKVTIARLL 782

BLAST of Cucsa.372010.2 vs. Swiss-Prot
Match: AGD1_ARATH (ADP-ribosylation factor GTPase-activating protein AGD1 OS=Arabidopsis thaliana GN=AGD1 PE=2 SV=2)

HSP 1 Score: 630.2 bits (1624), Expect = 3.0e-179
Identity = 358/830 (43.13%), Postives = 500/830 (60.24%), Query Frame = 1

Query: 4   FIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLEGFG 63
           F KL+DSPMF +Q+ S++ + ++L  RC R + GCR + E LGE  + DI F ++LE FG
Sbjct: 3   FAKLDDSPMFRQQMQSMEESAELLRLRCLRFYKGCRKYTEGLGEGYDADIGFVNALESFG 62

Query: 64  GGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSR 123
           GG +DPV V+ GGPV++KF  A RE+ TYKE LRSQVEH+L++RL+QF++ D+  VK++R
Sbjct: 63  GGHNDPVCVAFGGPVMTKFTIALREIGTYKEVLRSQVEHMLSDRLLQFVNGDVHEVKEAR 122

Query: 124 QRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAK 183
           +RFDKA   YDQAREK++SL+K+TR D+ A  EEDL ++++ FE++RF+LV+AL N E+K
Sbjct: 123 KRFDKATITYDQAREKYLSLRKSTRLDVAATIEEDLHSARTTFEQARFHLVSALSNAESK 182

Query: 184 KKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQ 243
           K++EFLE++S  MDAHLR+FK G++L  QMEPFI+QVL YA QS+E AN E   L +R+Q
Sbjct: 183 KRFEFLEAVSGTMDAHLRFFKQGYELLHQMEPFINQVLAYAHQSRECANYEMASLNERMQ 242

Query: 244 EFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKR 303
           E+  Q Q+D  +  NS   S   DG+  +  NS + +E   QS   GKV+TI+QGYL KR
Sbjct: 243 EY--QRQVDR-ETRNSC-VSPTGDGMRHNSRNSQKVIEAVMQSAAKGKVQTIRQGYLSKR 302

Query: 304 SSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSF---NRSDEHN----------- 363
           SS  RGDWKR FF+LDS+G LYYY+   +   G  S S    N + E++           
Sbjct: 303 SSNLRGDWKRRFFILDSRGMLYYYRKPWNWSSGNGSRSVVHRNMASENSPGLLSRWLSSH 362

Query: 364 --------------NINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 423
                          +NL TSTIK+DA+ +DLR CFRIISP+K +TLQAEN  D+MDWI 
Sbjct: 363 YHGGVHDEKPVARHTVNLLTSTIKVDADQTDLRFCFRIISPTKVYTLQAENAQDQMDWIE 422

Query: 424 KITGAIASLFNSYFLQQPFLEAKHPE-------CDKNNFND-YSVQTRH--EKRLE---- 483
           KITG IASL +    ++  +     +        D  +  D Y ++     E  +E    
Sbjct: 423 KITGVIASLLSFQTPERAIMRLSTVDGGDTFSASDSGSLADPYDIEQAESGESTVEHPMT 482

Query: 484 --------------DGSQVDSVSKILREIPGNDLCAECGSSEPEWASLNLGILLCIECSG 543
                         D  + +    +L  + GN+ CA+CG+ EP+WASLNLG+L+CIECSG
Sbjct: 483 GGNRSRFSGCLQQHDMVKTEKPIDVLTRVLGNERCADCGAPEPDWASLNLGVLICIECSG 542

Query: 544 VHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALA 603
           +HRNLGVHISKVRS+ LDVKVWEPS+L LF++LGN Y NS+WE L  +++   +   +  
Sbjct: 543 IHRNLGVHISKVRSLTLDVKVWEPSVLTLFQSLGNVYVNSVWEELLNSESRTSSASRSSG 602

Query: 604 TS---------IPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAKSIWEAVKT 663
           T          + KP   D   ++E +I AKY E+ FV K  ++    +  + IWE V+ 
Sbjct: 603 TPKSDRPRKLLVRKPGFNDPISVKELFIHAKYSERIFVRKAIDSQHFQAVFQEIWENVRA 662

Query: 664 NDLREAYRLIAVSAVSI----VNTTYDNVVSVSSSPHLDEEPSGNQESL------NP--- 723
           ND +  YR I  S   +       +Y   + +S    ++E      +S+      NP   
Sbjct: 663 NDKKSVYRHIVCSEADVNALRGQASYTVSLPLSKMMQMEETLEAKFKSIEEEFQENPAGY 722

Query: 724 SSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCIS 756
           S+   D +S    ++   CSLLHLAC + +  M+ELLLQ+G  IN  DS GRTPLH CI 
Sbjct: 723 SNSRGDGESMVREETSNDCSLLHLACLSADIGMVELLLQYGAKINATDSKGRTPLHHCII 782

BLAST of Cucsa.372010.2 vs. Swiss-Prot
Match: ACAP2_CHICK (Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 OS=Gallus gallus GN=ACAP2 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.4e-59
Identity = 211/782 (26.98%), Postives = 335/782 (42.84%), Query Frame = 1

Query: 7   LEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLEGFGGGQ 66
           L+DSP F   +  ++     LE +  +L   C   ++T G+A      F  + + F  G 
Sbjct: 11  LKDSPRFRAALEEVEGDVAELELKLDKLVKLCIAMIDT-GKA------FCLANKQFMNGI 70

Query: 67  DDPVSVSIGGPV----LSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDS 126
            D    S    +    L+KF    +E+  Y   L  Q +  +  +L  F+ ED++  KD+
Sbjct: 71  RDLAQYSCKDALVETNLTKFSDTLQEMINYHNILFDQTQRSIKAQLQTFVKEDIKKFKDA 130

Query: 127 RQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEA 186
           +++F+K   E + A  K   +++N + + V E    L  ++  F     + V  +  +++
Sbjct: 131 KKQFEKVSEEKENALVKNAQVQRNKQHE-VEEATNILTATRKCFRHIALDYVLQINVLQS 190

Query: 187 KKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRI 246
           K++ E L+S+ + M AHL +F  G+DLF ++ P++          K+L            
Sbjct: 191 KRRSEILKSMLSFMYAHLTFFHQGYDLFSELGPYM----------KDLG----------- 250

Query: 247 QEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLK 306
                 AQLD L    + +   +           Y   +T  +  V+     + +GYL K
Sbjct: 251 ------AQLDQLAVDAAKEKRDMEQKHSTIQQKDYSGDDTKLEYNVDAANGIVMEGYLFK 310

Query: 307 RSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNNINLHTSTIKMDA 366
           R+S +   W R +F + +   +Y  K K +  V  +             +L   T+K   
Sbjct: 311 RASNAFKTWNRRWFSIQNNQLVYQKKFKDNPTVVVE-------------DLRLCTVK-HC 370

Query: 367 EDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFLQQPFLEAKHPEC 426
           ED + R CF ++SP+K+  LQA++E  R  WI  +  +IA+ +     +    E +  + 
Sbjct: 371 EDIERRFCFEVVSPTKSCMLQADSEKLRQAWIKAVQTSIATAYREKGDES---EKQEKKS 430

Query: 427 DKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEPEWASLNLGILLC 486
             +  +  S     EK L+  S +  V  I    PGN  C +CG ++P WAS+NLGI LC
Sbjct: 431 SPSTGSLESGSETKEKLLKGESALQRVQCI----PGNAACCDCGLADPRWASINLGITLC 490

Query: 487 IECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWEGLFLADTGRDNT 546
           IECSG+HR+LGVH SKVRS+ LD   WEP +L L   LGN   N I+E   L   G    
Sbjct: 491 IECSGIHRSLGVHFSKVRSLTLDS--WEPELLKLMCELGNDVINRIYEAK-LEKMG---- 550

Query: 547 QHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAP-----GNISYAKSIWEAVK 606
                  + KP P    Q +E YI+AKYVE++FV K   A                E  +
Sbjct: 551 -------VKKPQPGSQRQEKEMYIKAKYVERKFVEKQPAAAVSPLESRTKVLPQSQEEKR 610

Query: 607 TNDLREAYRLIAVSAVS------------IVNTTYDNVVSVSSSPHLDEEPSGNQES--- 666
            +   +++  I   A S             V+ + +++ S  S+  L E     QES   
Sbjct: 611 HSAPEKSFLAIEQGAASPRVRSSDSGIQQSVDDSREHLASTISANSLYEPEGEKQESSVF 670

Query: 667 -----LNPS---------------------SCTRDWDSNESSDSLQGCSLLHLACQNDNQ 726
                LNP                          +W + E + +      L  A +  + 
Sbjct: 671 YDSRQLNPGLHLYRAAFEKNLPDMAEALAHGAEVNWVNMEENKATP----LIQAVRGGSL 718

Query: 727 VMLELLLQFGTDINVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERA 739
           V  E LLQ G ++N+ D  GR PLH      +     L L+RGA     D  G   L  A
Sbjct: 731 VTCEFLLQNGANVNIRDMKGRGPLHHATVLGHTGQVCLFLKRGANQHATDEDGKDPLSIA 718

BLAST of Cucsa.372010.2 vs. TrEMBL
Match: A0A0A0KJC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G053060 PE=4 SV=1)

HSP 1 Score: 1343.2 bits (3475), Expect = 0.0e+00
Identity = 679/705 (96.31%), Postives = 680/705 (96.45%), Query Frame = 1

Query: 76  GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ 135
           GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ
Sbjct: 34  GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ 93

Query: 136 AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL 195
           AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL
Sbjct: 94  AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL 153

Query: 196 MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ 255
           MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ
Sbjct: 154 MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ 213

Query: 256 ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF 315
           ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF
Sbjct: 214 ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF 273

Query: 316 FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN------------------------- 375
           FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN                         
Sbjct: 274 FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNNSMFGRFRARHNRSSSLNEEILRCST 333

Query: 376 INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL 435
           +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL
Sbjct: 334 VNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL 393

Query: 436 QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP 495
           QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP
Sbjct: 394 QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP 453

Query: 496 EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE 555
           EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE
Sbjct: 454 EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE 513

Query: 556 GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK 615
           GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK
Sbjct: 514 GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK 573

Query: 616 SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR 675
           SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR
Sbjct: 574 SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR 633

Query: 676 DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK 735
           DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK
Sbjct: 634 DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK 693

Query: 736 LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 694 LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 738

BLAST of Cucsa.372010.2 vs. TrEMBL
Match: A0A061E8Q4_THECC (Gcn4-complementing protein, putative isoform 1 OS=Theobroma cacao GN=TCM_011296 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 2.5e-281
Identity = 507/795 (63.77%), Postives = 604/795 (75.97%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M AFIKLEDSPMF KQ+ SL+ T D L+DRC+RL+ G + FM  LGEA NG+ SFADSLE
Sbjct: 1   MAAFIKLEDSPMFQKQLCSLEFTADELKDRCQRLYKGSKKFMAALGEAYNGETSFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGGQDDPVSVSIGGP++SKF+ AFRELA+YKE LRSQVEHVL +RLM F+  DLQ  K
Sbjct: 61  AFGGGQDDPVSVSIGGPIMSKFINAFRELASYKELLRSQVEHVLIDRLMHFMTVDLQEAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+RFDKA++ YDQAREKFVSLK+NTRGDIVAE EEDL NSKSAFE+SRFNLVNALMN+
Sbjct: 121 ESRRRFDKAINAYDQAREKFVSLKRNTRGDIVAELEEDLQNSKSAFERSRFNLVNALMNI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MDAHLRYFKLG+DL  Q+EPFIHQVLTYAQQSKELAN EQDKL K
Sbjct: 181 EAKKKYEFLESISAIMDAHLRYFKLGYDLLSQLEPFIHQVLTYAQQSKELANAEQDKLEK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQA++D+L+AS++L+ S  +D IH  G+NS +++E   QS  NG+V+TIKQGYL
Sbjct: 241 RIQEFRTQAEIDSLRASSNLEPSTSADRIHVIGMNSDKNIEAIMQSSTNGEVQTIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSH-----------------SFN 360
           LKRSS  RGDWKR FFVLDSQG LYYY+NK  + +G   H                   N
Sbjct: 301 LKRSSSLRGDWKRRFFVLDSQGTLYYYRNKGIKPMGSHHHYTGSAEQNSGVFARFRARHN 360

Query: 361 RSDEHN-------NINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINK 420
           RS   N        ++LHTSTIKMDAED+DLRLCFRIISP KT+TLQAEN ADRMDW+NK
Sbjct: 361 RSSSFNEETLGCHTVDLHTSTIKMDAEDTDLRLCFRIISPLKTYTLQAENGADRMDWVNK 420

Query: 421 ITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLED----------GSQV 480
           IT  I SL NS+ LQQ             + NDY+ +   + R  D          G++ 
Sbjct: 421 ITAVITSLLNSHILQQHV-----------DNNDYACRATSDVRSLDSLGSLEIDRIGNRA 480

Query: 481 DSVSKILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDV 540
           + VS + REIPGND+CAEC + EP+WASLNLGILLCIECSGVHRNLGVHISKVRS+ LDV
Sbjct: 481 EPVSSVFREIPGNDICAECSAPEPDWASLNLGILLCIECSGVHRNLGVHISKVRSLTLDV 540

Query: 541 KVWEPSILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYI 600
           KVWEPSI++LFR LGN YCNS+WEG  L +   D+T +A++TSI KP  KDA   +E+YI
Sbjct: 541 KVWEPSIVELFRTLGNAYCNSVWEGSLLKNERVDDT-NAISTSIIKPCAKDAISHKEKYI 600

Query: 601 QAKYVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSV 660
            AKYVEK  +++D   PG    + +IW+AVKT++LRE YRLIA+S  +I+NTT+D+VVS+
Sbjct: 601 HAKYVEKLLIIRDGTLPGVPPNSTNIWQAVKTDNLREVYRLIAMSDTNIINTTFDDVVSI 660

Query: 661 SSSPHLDEEPSG------NQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLE 720
            S  H+D + S        ++  +PS+C R  DSN+  + LQGCS+LHLACQ  N VM+E
Sbjct: 661 ESYHHIDAQDSSLDGHKEERKQYDPSACQRIKDSNDPGNCLQGCSVLHLACQCGNPVMVE 720

Query: 721 LLLQFGTDINVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMG 756
           LLLQFG DIN+ D HGRTPLH CIS  NN  AK LLRRGA+PSIKD GGLSALERAME G
Sbjct: 721 LLLQFGADINMRDFHGRTPLHYCISIGNNTFAKHLLRRGARPSIKDGGGLSALERAMEKG 780

BLAST of Cucsa.372010.2 vs. TrEMBL
Match: M5XRV9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001640mg PE=4 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 1.6e-280
Identity = 500/789 (63.37%), Postives = 604/789 (76.55%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M AFIKLEDSPMF KQI SL++T D L+DRC++L+ GC+ FM  LGEA NGD +FADSLE
Sbjct: 1   MAAFIKLEDSPMFQKQIYSLEQTADELKDRCQKLYKGCKKFMAALGEACNGDTTFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGG DDPVSVSIGGPV+SKFVTAF+ELATYKE LRSQVEHVL +RLM F+  D+Q  K
Sbjct: 61  AFGGGLDDPVSVSIGGPVMSKFVTAFQELATYKELLRSQVEHVLVDRLMNFMTVDMQDAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+RFDKA+H YDQAREKFVSLKKNTRGDIVAE EEDL NSKS FEKSRFNLVN+LM++
Sbjct: 121 ESRRRFDKAIHGYDQAREKFVSLKKNTRGDIVAELEEDLQNSKSTFEKSRFNLVNSLMSI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           E+KKKYEFLESISA+MDAHLRYFKLG++LF QMEP+IHQVLTYAQQSKE + +EQ+KL K
Sbjct: 181 ESKKKYEFLESISAIMDAHLRYFKLGYELFSQMEPYIHQVLTYAQQSKEQSTVEQEKLHK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQA+LD+++AS++++ S   DG    G+ SY++++   QS  NGKV+TIKQGYL
Sbjct: 241 RIQEFRTQAELDSVRASSNIEPSAAVDGNRAFGLASYKNIDAIMQSSANGKVQTIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSS  RGDWKR FFVL++QG+L+YY+ K ++ +G QSH F RS EH++          
Sbjct: 301 LKRSSSLRGDWKRRFFVLNNQGSLFYYRIKGTKPMGSQSHHFTRSPEHHSGVFGRFRSRH 360

Query: 361 --------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINK 420
                         ++L TSTIKMDAED+DLRLCFRIISP KT+TLQAEN+ADRMDWINK
Sbjct: 361 RGPSLNENILGCRTVDLCTSTIKMDAEDTDLRLCFRIISPLKTYTLQAENDADRMDWINK 420

Query: 421 ITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDG----SQVDSVSKI 480
           ITGAI SL NS  L+QP     + +  K+    Y V   +     +     +++DSVS I
Sbjct: 421 ITGAIQSLLNSQLLEQPHPRNMNLKSSKSGLGTYGVHPLNSNESREDTIKFNRIDSVSSI 480

Query: 481 LREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPS 540
           LREIPGNDLCAEC + EP+WASLNLGIL+CIECSGVHRNLGVHISKVRS+ LDVKVWEP+
Sbjct: 481 LREIPGNDLCAECSAPEPDWASLNLGILVCIECSGVHRNLGVHISKVRSLTLDVKVWEPT 540

Query: 541 ILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVE 600
           I+DLFRNLGN YCNS+WE   L +  R N   A   SI KP P++A Q +E YIQAKYVE
Sbjct: 541 IVDLFRNLGNAYCNSLWERTLLLENERANGSKATRASILKPGPENAIQHKEIYIQAKYVE 600

Query: 601 KQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHL 660
           K  VV+++   G    A SIW+AVKT +L+E YRLI +S V+I++TT+DNVV V    H 
Sbjct: 601 KVMVVREA-VSGMPLLAASIWQAVKTCNLQEVYRLIVISDVNIIDTTFDNVVGVDLYHHA 660

Query: 661 DEEP------SGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFG 720
           D +       +   +  +P++C R  D+NE  + LQGCSLLHLAC   N +M+ELLLQFG
Sbjct: 661 DAQDLESDFHTMEMKQHDPAACERIKDANEPGNCLQGCSLLHLACDCGNPMMIELLLQFG 720

Query: 721 TDINVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEE 756
            +IN+ D HGRTPLH+CIS  NN LAK LLRRGA+PSI+D GG SALERAMEMGAITDEE
Sbjct: 721 ANINLRDFHGRTPLHRCISSGNNSLAKFLLRRGARPSIQDGGGQSALERAMEMGAITDEE 780

BLAST of Cucsa.372010.2 vs. TrEMBL
Match: A0A067KDT3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_18591 PE=4 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 6.7e-279
Identity = 495/788 (62.82%), Postives = 606/788 (76.90%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M AFI L DSPMF K+I SL+R  + L +RC++L  G R +M TLG+A   D +FADSLE
Sbjct: 1   MAAFINLHDSPMFQKEICSLERLAEELNERCQKLCKGSRRYMATLGDACIADTTFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGGQDDPVSVSIGGPVLSKF+ AFRELATYKE L SQVEHVL +RL+QF+D DL+  K
Sbjct: 61  AFGGGQDDPVSVSIGGPVLSKFINAFRELATYKELLCSQVEHVLIDRLVQFMDVDLREAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+RFDKA++ YDQ+REKFVSLK+NTRGDI+ E EEDL NSKSAFEKSRFNLV+AL+N+
Sbjct: 121 ESRKRFDKAIYAYDQSREKFVSLKRNTRGDIIEELEEDLQNSKSAFEKSRFNLVSALVNI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MDAHLRYFKLG DL  QMEPFIHQVLTYAQQSKELA+IEQDKLAK
Sbjct: 181 EAKKKYEFLESISAIMDAHLRYFKLGHDLLSQMEPFIHQVLTYAQQSKELASIEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQA+ DN ++S++++ S  +DGIH  G++SY ++    QS  NG+V+TIKQGYL
Sbjct: 241 RIQEFRTQAEFDNNRSSSNIEPSTSADGIHVVGMSSYENIGAIMQSSANGEVQTIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSS  R DWKR FFVLDSQG+LYYY+NKA++ VG Q H    S +HN+          
Sbjct: 301 LKRSS-HRADWKRRFFVLDSQGSLYYYRNKAAKPVGFQ-HRSTVSGDHNSSVFARFRSRH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          I+L TS +KMDAED++LRLCFR+ISP KT+TLQAEN ADRMDW+N
Sbjct: 361 NRASSLSEGSLDCCIIDLRTSALKMDAEDTNLRLCFRVISPQKTYTLQAENGADRMDWVN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDK-NNFNDYSVQTRHEKRLEDG----SQVDSVS 480
           KITG IASLFNS+FLQQP    KH E ++  +    +VQ    +   DG    S+ + VS
Sbjct: 421 KITGVIASLFNSHFLQQPHPRMKHLENNECTSDASTAVQQLDNQPNLDGSVEVSRANCVS 480

Query: 481 KILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 540
           +ILREIPGN++CAEC + EP+WASLNLGILLCIECSGVHRNLGVHISKVRS+ LDVKVWE
Sbjct: 481 RILREIPGNEICAECSALEPDWASLNLGILLCIECSGVHRNLGVHISKVRSLTLDVKVWE 540

Query: 541 PSILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKY 600
           P++LDLF++LGN YCNSIWEGL L    R++  +++A+SI KP PKDA   +E+YI  KY
Sbjct: 541 PTVLDLFQSLGNVYCNSIWEGLLLLKNEREDESNSIASSIQKPCPKDAIYCKEKYIHTKY 600

Query: 601 VEKQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSP 660
           VEK  V++++   GN+S++  IW+AVKTN+ RE YR IA+S ++IVNTT+D V  V S  
Sbjct: 601 VEKLLVIREASVSGNLSHSLGIWQAVKTNNFREVYRYIAISDINIVNTTFDEVFGVESLN 660

Query: 661 HLDE-------EPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLL 720
           H+++           +++  +P++C R  DSN+  + LQGCSLLHLAC   N VMLELLL
Sbjct: 661 HVNDMQNPEFNSNEADRKQYDPAACQRIKDSNDQGNCLQGCSLLHLACHYGNPVMLELLL 720

Query: 721 QFGTDINVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAIT 752
           QFG D+N+ D HGRTPLH CIS++N  LAK LLRRGA PS++DCGG S LERAMEMGAIT
Sbjct: 721 QFGADVNLRDFHGRTPLHHCISKRNYSLAKFLLRRGASPSVRDCGGQSVLERAMEMGAIT 780

BLAST of Cucsa.372010.2 vs. TrEMBL
Match: B9RY53_RICCO (Gcn4-complementing protein, putative OS=Ricinus communis GN=RCOM_0814510 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 1.5e-278
Identity = 491/793 (61.92%), Postives = 598/793 (75.41%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M AFI LEDSPMF K+I SL++ ++ + DRC+RL+ GC+ FM  LG+A N D +FA+SLE
Sbjct: 1   MAAFINLEDSPMFQKEICSLEQISEEMNDRCQRLYKGCKSFMAALGDACNADTTFAESLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGG DDPVSVSIGGPV+SKF+ AFRELATYKE LRSQVEHVL +RL+ F+D DLQ  K
Sbjct: 61  AFGGGHDDPVSVSIGGPVISKFINAFRELATYKELLRSQVEHVLIDRLVHFMDVDLQNAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+R+DKA+H YDQ+REKFVSLKKNTRG+I+ E EED+ NSKSAFE+SRFNLV+AL+N+
Sbjct: 121 ESRKRYDKAIHAYDQSREKFVSLKKNTRGNIIEELEEDMQNSKSAFERSRFNLVSALVNI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MDAHLRYFKLG++L  QMEPFIHQVLTYAQQSKELAN EQDKLAK
Sbjct: 181 EAKKKYEFLESISAIMDAHLRYFKLGYELLSQMEPFIHQVLTYAQQSKELANSEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQA+ +++ AS++++ S  +DGIH  G++S++++E    S   G+V  IKQGYL
Sbjct: 241 RIQEFRTQAEFNSIHASSNIEPSTSADGIHVVGMSSFKNIEAIMHSTAKGEVLIIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSS  RGDWKR FFVLDSQG LYYY+NKA++ VG Q H    S EHN+          
Sbjct: 301 LKRSSRLRGDWKRRFFVLDSQGTLYYYRNKATKPVGFQ-HRSTASIEHNSSVFARFRSRH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          I+L TSTIKM+AED+DLRLCFR+ISP KT+TLQAENE DRMDW+N
Sbjct: 361 NRASSLGEGSLACRIIDLRTSTIKMEAEDTDLRLCFRVISPLKTYTLQAENETDRMDWVN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQ---------- 480
           KITG IASL  ++F+QQP+   KHPEC     NDY+  T    +  DG Q          
Sbjct: 421 KITGVIASLLKAHFMQQPYPGMKHPEC-----NDYTSGTTCVVQQLDGHQRLGDDLKVNR 480

Query: 481 VDSVSKILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILD 540
            D VS +LR+IPGNDLCAEC + EP+WASLNLGIL+CIECSGVHRNLGVHISKVRS+ LD
Sbjct: 481 ADCVSSVLRKIPGNDLCAECSAPEPDWASLNLGILVCIECSGVHRNLGVHISKVRSLTLD 540

Query: 541 VKVWEPSILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERY 600
           VKVWEP++LDLF  LGN YCNSIWEGL +    R +   A A+SI KP PKD    +E+Y
Sbjct: 541 VKVWEPTVLDLFHALGNAYCNSIWEGLLMLRNERVDEPSAFASSIEKPCPKDVIFCKEKY 600

Query: 601 IQAKYVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVS 660
           IQAKYVEK  V++++   G+ S+A  IW+AVKTN+LRE YR I +S ++IVNTT+D VV 
Sbjct: 601 IQAKYVEKLLVIREASVSGSFSHASGIWQAVKTNNLREIYRHIVISDINIVNTTFDEVVG 660

Query: 661 VSSSPHLDE-------EPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVM 720
           + S  H+ +         +  ++  +P++C R  DS +  + LQGCSLLHLAC   N VM
Sbjct: 661 IESLHHVSDTQDSQFNSHTSERKQHDPATCPRIKDSKDPENCLQGCSLLHLACHYGNPVM 720

Query: 721 LELLLQFGTDINVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAME 752
           LELLLQFG D+N+ D H RTPLH CIS+ N  LAK LLRRGA PS++D GGLS LERAME
Sbjct: 721 LELLLQFGADVNLRDFHHRTPLHHCISKGNYPLAKFLLRRGASPSVRDGGGLSVLERAME 780

BLAST of Cucsa.372010.2 vs. TAIR10
Match: AT1G10870.1 (AT1G10870.1 ARF-GAP domain 4)

HSP 1 Score: 879.0 bits (2270), Expect = 2.1e-255
Identity = 457/779 (58.66%), Postives = 561/779 (72.02%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M  FI LEDSPMF KQ+ SL+ T D L+DRC++L+ G + FM TLGEAS G+ +FA  LE
Sbjct: 1   MATFINLEDSPMFQKQVCSLEGTADELKDRCQKLYKGVKKFMGTLGEASKGESAFAACLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FGGG DDP+S+SIGGPV+SKF+ A RELA+YKE L SQVEHVL  RLM F+  DLQ  K
Sbjct: 61  EFGGGPDDPISLSIGGPVISKFINALRELASYKEFLCSQVEHVLLERLMNFISVDLQEAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR RFDKA H YDQ+REKFVSLKKNTRG+IVAE EEDL NSKS FEKSRFNLVN+LM +
Sbjct: 121 ESRHRFDKAAHSYDQSREKFVSLKKNTRGEIVAELEEDLENSKSTFEKSRFNLVNSLMTI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MDAHLRYFKLG+DL  Q+EPFIHQ+LTYAQQSKE + IEQD+LA+
Sbjct: 181 EAKKKYEFLESISAIMDAHLRYFKLGYDLLNQLEPFIHQILTYAQQSKEQSKIEQDRLAR 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQ++LD+ Q   + ++S  +    G  I  Y++ ET+    +    E IKQGYL
Sbjct: 241 RIQEFRTQSELDSQQLVANAESSGANGNRVGGNI-PYKNTETS----LTADKEVIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDE------------- 360
           LKRSS  R DWKR FFVLDS G++YYY+   ++ +G   H    SD              
Sbjct: 301 LKRSSSLRTDWKRKFFVLDSHGSMYYYRTNGNKSMGSHHHYSGSSDHNTGVFGRFRARHN 360

Query: 361 -----------HNNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINK 420
                      +N I+L TS IK+DAED DLRLCFRIISP KT+TLQAEN ADRMDW+NK
Sbjct: 361 RSGSLTEGSLGYNTIDLRTSLIKLDAEDMDLRLCFRIISPQKTYTLQAENGADRMDWVNK 420

Query: 421 ITGAIASLFNSYFLQQP---FLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKIL 480
           IT AI +L NS+FLQQ    +L+  +      N      Q RH    ++    D VS IL
Sbjct: 421 ITKAIGTLLNSHFLQQSPVRYLDKDNSSSAPANAVVSGDQIRHNDSRQNIG--DDVSTIL 480

Query: 481 REIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSI 540
           R +PGN+ CAEC + EP+WASLNLG+LLCI+CSGVHRNLGVHISKVRS+ LDVKVWEP+I
Sbjct: 481 RGLPGNNACAECNAPEPDWASLNLGVLLCIQCSGVHRNLGVHISKVRSLSLDVKVWEPTI 540

Query: 541 LDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEK 600
           LDLFRNLGN YCNS+WEGL   D   ++       S+ KP P+D+  ++E+YI  KY+EK
Sbjct: 541 LDLFRNLGNVYCNSLWEGLLHLDDDCEDGSALSHASVSKPCPEDSFSVKEKYILGKYLEK 600

Query: 601 QFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSA-VSIVNTTYDNVVSVSSSPHL 660
             V+KD E+  N+S A  IWEAV++ ++RE YRLI  +  V+I+NT +D++  + +  H+
Sbjct: 601 ALVIKD-ESEANLSAASRIWEAVQSRNIREIYRLIVTTGDVNIINTKFDDITDIDAYHHI 660

Query: 661 DEEPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVC 720
           D      ++  +P+ C R  +SNE    LQGCSLLH+AC   + V+LELLLQFG D+N+ 
Sbjct: 661 DAAEKAVKKRHDPTVCQRIKESNEPRSCLQGCSLLHVACHIGDSVLLELLLQFGADLNIR 720

Query: 721 DSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLL 752
           D HGRTPLH CIS  N+K AK+LLRRGA+PSI+D GGLS LERAMEMGAITDEELFLLL
Sbjct: 721 DYHGRTPLHHCISSGNHKFAKILLRRGARPSIEDDGGLSVLERAMEMGAITDEELFLLL 771

BLAST of Cucsa.372010.2 vs. TAIR10
Match: AT1G60860.1 (AT1G60860.1 ARF-GAP domain 2)

HSP 1 Score: 859.8 bits (2220), Expect = 1.3e-249
Identity = 461/783 (58.88%), Postives = 563/783 (71.90%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           M  FI LEDSPMF KQ+ SL+ T+D L+DRC++L+ G + FM  LGEAS G  +FADSLE
Sbjct: 1   MAGFINLEDSPMFQKQVFSLEGTSDELKDRCQKLYKGVKKFMGALGEASTGVSAFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
            FG G DDPVSVSIGGPV+SKF+   REL++YKE LRSQVEHVL  RL  F+  DLQ  K
Sbjct: 61  EFGAGHDDPVSVSIGGPVISKFINTLRELSSYKEFLRSQVEHVLLERLTNFMTVDLQEAK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           +SR+RFDKA+H YDQAREKFVSLKKNTRGDIVAE EEDL NSKSAFEKSRFNLVN+LM +
Sbjct: 121 ESRRRFDKAVHSYDQAREKFVSLKKNTRGDIVAELEEDLENSKSAFEKSRFNLVNSLMTI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISA+MD+H +YFKLG+DL  Q+EP+IHQVLTYAQQSKE + IEQD+ A+
Sbjct: 181 EAKKKYEFLESISAIMDSHFKYFKLGYDLLSQLEPYIHQVLTYAQQSKEQSKIEQDRFAQ 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSV---ETAPQSIVNGKVETIKQ 300
           RIQEFRTQ++LD+ QAS   D S V       G + YR++        S+     E  KQ
Sbjct: 241 RIQEFRTQSELDSQQASAKADPSDV------GGNHVYRAIPRKNVEANSVSTADKEVTKQ 300

Query: 301 GYLLKRSSGSRGDWKRMFFVLDSQGALYYYKNKASR-------YVGQQSHS--------- 360
           GYLLKRS+  R DWKR FFVLD+ G+LYYY+N  ++       Y G   HS         
Sbjct: 301 GYLLKRSASLRADWKRRFFVLDNHGSLYYYRNTGNKSAKSQHYYSGLGEHSSGVFGRFRT 360

Query: 361 -FNRSDEH-----NNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
             NRS        N I+L TS IK+DAED+DLRLCFRIISP KT+TLQAEN ADRMDW+N
Sbjct: 361 RHNRSASQGSLDCNMIDLRTSLIKLDAEDTDLRLCFRIISPQKTYTLQAENGADRMDWVN 420

Query: 421 KITGAIASLFNSYFLQQP---FLEAKHPECDKNNFN-DYSVQTRHEKRLEDGSQVDSVSK 480
           KIT AI    NS+FLQQ    +L+ K+        N   + +  + +RL  G   D V  
Sbjct: 421 KITAAITIRLNSHFLQQSPARYLDKKNTSSGPATENLTLNQKEDYNQRLNVG---DDVLT 480

Query: 481 ILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEP 540
           ILREIPGN+ CAEC + +P+WASLNLG+L+CIECSGVHRNLGVHISKVRS+ LDVKVWEP
Sbjct: 481 ILREIPGNNTCAECNAPDPDWASLNLGVLMCIECSGVHRNLGVHISKVRSLTLDVKVWEP 540

Query: 541 SILDLFRNLGNTYCNSIWEGLF--LADTGRDNTQHALATSIPKPSPKDANQLRERYIQAK 600
           +ILDLFRNLGN YCNS+WE L   L D     +   LA S+ KPS +D   L+E+YI  K
Sbjct: 541 TILDLFRNLGNGYCNSVWEELLHHLDDDSEKGSTDTLA-SVSKPSSEDWFTLKEKYINGK 600

Query: 601 YVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSS 660
           Y+EK  VVKD E   N + +  IWEAV++ ++R+ YRLI  +  +I+NT +D++  +   
Sbjct: 601 YLEKALVVKD-EREANSTASSRIWEAVQSRNIRDIYRLIVKADANIINTKFDDITDLDVY 660

Query: 661 PHLD-EEPSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTD 720
            H   + P   ++  +P++C R  +SNE+ + LQGCSLLH+ACQ+ + ++LELLLQFG D
Sbjct: 661 HHHHVDAPDEVKKRHDPNACQRIKNSNEARNCLQGCSLLHVACQSGDPILLELLLQFGAD 720

Query: 721 INVCDSHGRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELF 752
           IN+ D HGRTPLH CI+  NN  AK+LLRRGA+PSI+D GGLS LERAMEMGAITDEELF
Sbjct: 721 INMRDYHGRTPLHHCIASGNNAFAKVLLRRGARPSIEDGGGLSVLERAMEMGAITDEELF 772

BLAST of Cucsa.372010.2 vs. TAIR10
Match: AT5G13300.1 (AT5G13300.1 ARF GTPase-activating protein)

HSP 1 Score: 671.4 bits (1731), Expect = 6.5e-193
Identity = 375/817 (45.90%), Postives = 507/817 (62.06%), Query Frame = 1

Query: 4   FIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLEGFG 63
           F KL+DSPMF KQ+ S++ + ++L +R  + + GCR + E LGEA +GDI+FA +LE FG
Sbjct: 3   FTKLDDSPMFRKQLQSMEESAEILRERSLKFYKGCRKYTEGLGEAYDGDIAFASALETFG 62

Query: 64  GGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSR 123
           GG +DP+SV+ GGPV++KF  A RE+ TYKE LRSQVEH+L +RL+QF + DL  VK++R
Sbjct: 63  GGHNDPISVAFGGPVMTKFTIALREIGTYKEVLRSQVEHILNDRLLQFANMDLHEVKEAR 122

Query: 124 QRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAK 183
           +RFDKA   YDQAREKF+SL+K T+ D+ A  E++L  S+S FE++RFNLV AL NVEAK
Sbjct: 123 KRFDKASLTYDQAREKFLSLRKGTKSDVAAALEQELHTSRSMFEQARFNLVTALSNVEAK 182

Query: 184 KKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQ 243
           K++EFLE++S  MDAHLRYFK G++L  QMEP+I+QVLTYAQQS+E +N EQ  L +++Q
Sbjct: 183 KRFEFLEAVSGTMDAHLRYFKQGYELLHQMEPYINQVLTYAQQSRERSNYEQAALNEKMQ 242

Query: 244 EFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKR 303
           E++ Q   ++   SN  + S   DGI   G +S++ ++   QS   GKV+TI+QGYL KR
Sbjct: 243 EYKRQVDRESRWGSNGSNGSPNGDGIQAIGRSSHKMIDAVMQSAARGKVQTIRQGYLSKR 302

Query: 304 SSGSRGDWKRMFFVLDSQGALYYYKNKA------------------------SRYVGQQS 363
           SS  RGDWKR FFVLDS+G LYYY+ +                         SR++   +
Sbjct: 303 SSNLRGDWKRRFFVLDSRGMLYYYRKQCSKPSGSGSQLSGQRNSSELGSGLLSRWLSSNN 362

Query: 364 HSF-----NRSDEHNNINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWI 423
           H        +S   + +NL TSTIK+DA+ SDLR CFRIISP+K +TLQAE+  D+MDWI
Sbjct: 363 HGHGGVHDEKSVARHTVNLLTSTIKVDADQSDLRFCFRIISPTKNYTLQAESALDQMDWI 422

Query: 424 NKITGAIASLFNSYFLQQ-----PFLEAKH---PECDKNNFNDYSVQTRHE--------- 483
            KITG IASL +S   +Q     P     H    E      ++Y   T  E         
Sbjct: 423 EKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCERSFLG 482

Query: 484 ------KRLEDGSQVDSVSK---ILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGV 543
                 +  +    +    K    LR++ GND CA+CG+ EP+WASLNLG+L+CIECSGV
Sbjct: 483 YNERPSRSFQPQRSIRKGEKPIDALRKVCGNDKCADCGAPEPDWASLNLGVLVCIECSGV 542

Query: 544 HRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWEGLFLA------DTGRDNT 603
           HRNLGVHISKVRS+ LDVKVWEPS++ LF+ LGNT+ N++WE L  +      D G   +
Sbjct: 543 HRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIHFDPGLTVS 602

Query: 604 QHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAKSIWEAVKTNDLR 663
             +      KPS  D   ++E+YIQAKY EK FV +  ++    S A+ +W+AV  ND +
Sbjct: 603 DKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWDAVSGNDKK 662

Query: 664 EAYRLIAVSAVSIVNTTYDNVVSVSSSPH----LDEEPSGNQESLNPSSCTRD---WDSN 723
             YRLI V+  + VN  YD   S S +      + E P      L   +   D     SN
Sbjct: 663 AVYRLI-VNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELLDRTGSSSN 722

Query: 724 ESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNKLAKLL 753
            S +   G SLLH AC+  +  M+ELLLQ+G ++N  DS G+TPLH C+ +    +A+LL
Sbjct: 723 ISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRGKVTIARLL 782

BLAST of Cucsa.372010.2 vs. TAIR10
Match: AT5G61980.1 (AT5G61980.1 ARF-GAP domain 1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.7e-180
Identity = 358/830 (43.13%), Postives = 500/830 (60.24%), Query Frame = 1

Query: 4   FIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLEGFG 63
           F KL+DSPMF +Q+ S++ + ++L  RC R + GCR + E LGE  + DI F ++LE FG
Sbjct: 3   FAKLDDSPMFRQQMQSMEESAELLRLRCLRFYKGCRKYTEGLGEGYDADIGFVNALESFG 62

Query: 64  GGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSR 123
           GG +DPV V+ GGPV++KF  A RE+ TYKE LRSQVEH+L++RL+QF++ D+  VK++R
Sbjct: 63  GGHNDPVCVAFGGPVMTKFTIALREIGTYKEVLRSQVEHMLSDRLLQFVNGDVHEVKEAR 122

Query: 124 QRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAK 183
           +RFDKA   YDQAREK++SL+K+TR D+ A  EEDL ++++ FE++RF+LV+AL N E+K
Sbjct: 123 KRFDKATITYDQAREKYLSLRKSTRLDVAATIEEDLHSARTTFEQARFHLVSALSNAESK 182

Query: 184 KKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQ 243
           K++EFLE++S  MDAHLR+FK G++L  QMEPFI+QVL YA QS+E AN E   L +R+Q
Sbjct: 183 KRFEFLEAVSGTMDAHLRFFKQGYELLHQMEPFINQVLAYAHQSRECANYEMASLNERMQ 242

Query: 244 EFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKR 303
           E+  Q Q+D  +  NS   S   DG+  +  NS + +E   QS   GKV+TI+QGYL KR
Sbjct: 243 EY--QRQVDR-ETRNSC-VSPTGDGMRHNSRNSQKVIEAVMQSAAKGKVQTIRQGYLSKR 302

Query: 304 SSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSF---NRSDEHN----------- 363
           SS  RGDWKR FF+LDS+G LYYY+   +   G  S S    N + E++           
Sbjct: 303 SSNLRGDWKRRFFILDSRGMLYYYRKPWNWSSGNGSRSVVHRNMASENSPGLLSRWLSSH 362

Query: 364 --------------NINLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 423
                          +NL TSTIK+DA+ +DLR CFRIISP+K +TLQAEN  D+MDWI 
Sbjct: 363 YHGGVHDEKPVARHTVNLLTSTIKVDADQTDLRFCFRIISPTKVYTLQAENAQDQMDWIE 422

Query: 424 KITGAIASLFNSYFLQQPFLEAKHPE-------CDKNNFND-YSVQTRH--EKRLE---- 483
           KITG IASL +    ++  +     +        D  +  D Y ++     E  +E    
Sbjct: 423 KITGVIASLLSFQTPERAIMRLSTVDGGDTFSASDSGSLADPYDIEQAESGESTVEHPMT 482

Query: 484 --------------DGSQVDSVSKILREIPGNDLCAECGSSEPEWASLNLGILLCIECSG 543
                         D  + +    +L  + GN+ CA+CG+ EP+WASLNLG+L+CIECSG
Sbjct: 483 GGNRSRFSGCLQQHDMVKTEKPIDVLTRVLGNERCADCGAPEPDWASLNLGVLICIECSG 542

Query: 544 VHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWEGLFLADTGRDNTQHALA 603
           +HRNLGVHISKVRS+ LDVKVWEPS+L LF++LGN Y NS+WE L  +++   +   +  
Sbjct: 543 IHRNLGVHISKVRSLTLDVKVWEPSVLTLFQSLGNVYVNSVWEELLNSESRTSSASRSSG 602

Query: 604 TS---------IPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAKSIWEAVKT 663
           T          + KP   D   ++E +I AKY E+ FV K  ++    +  + IWE V+ 
Sbjct: 603 TPKSDRPRKLLVRKPGFNDPISVKELFIHAKYSERIFVRKAIDSQHFQAVFQEIWENVRA 662

Query: 664 NDLREAYRLIAVSAVSI----VNTTYDNVVSVSSSPHLDEEPSGNQESL------NP--- 723
           ND +  YR I  S   +       +Y   + +S    ++E      +S+      NP   
Sbjct: 663 NDKKSVYRHIVCSEADVNALRGQASYTVSLPLSKMMQMEETLEAKFKSIEEEFQENPAGY 722

Query: 724 SSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCIS 756
           S+   D +S    ++   CSLLHLAC + +  M+ELLLQ+G  IN  DS GRTPLH CI 
Sbjct: 723 SNSRGDGESMVREETSNDCSLLHLACLSADIGMVELLLQYGAKINATDSKGRTPLHHCII 782

BLAST of Cucsa.372010.2 vs. TAIR10
Match: AT3G07940.1 (AT3G07940.1 Calcium-dependent ARF-type GTPase activating protein family)

HSP 1 Score: 98.6 bits (244), Expect = 1.8e-20
Identity = 54/131 (41.22%), Postives = 76/131 (58.02%), Query Frame = 1

Query: 447 DSVSKILREIPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDV 506
           D + K+L++ PGN  CA+CGS EP+W SL+LG+ +CI+CSGVHR+LGVHISKV S+ LD 
Sbjct: 48  DRLEKLLKQ-PGNKYCADCGSPEPKWVSLSLGVFICIKCSGVHRSLGVHISKVLSVKLD- 107

Query: 507 KVWEPSILDLFRNL-GNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERY 566
             W    +D+     GNT  N  +E   +  +              KP P   N+ R  +
Sbjct: 108 -EWTDDQVDMLVGYGGNTAVNERFEACNIDQS-------------KKPKPDSTNEERNDF 162

Query: 567 IQAKYVEKQFV 577
           I+ KY + QF+
Sbjct: 168 IRKKYEQHQFM 162

BLAST of Cucsa.372010.2 vs. NCBI nr
Match: gi|778698234|ref|XP_011654489.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1491.9 bits (3861), Expect = 0.0e+00
Identity = 754/780 (96.67%), Postives = 755/780 (96.79%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE
Sbjct: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
           GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK
Sbjct: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV
Sbjct: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK
Sbjct: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL
Sbjct: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN          
Sbjct: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNNSMFGRFRARH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN
Sbjct: 361 NRSSSLNEEILRCSTVNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480
           KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE
Sbjct: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480

Query: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540
           IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD
Sbjct: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540

Query: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600
           LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF
Sbjct: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600

Query: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660
           VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE
Sbjct: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660

Query: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720
           PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH
Sbjct: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720

Query: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 780

BLAST of Cucsa.372010.2 vs. NCBI nr
Match: gi|778698238|ref|XP_011654490.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD4 isoform X2 [Cucumis sativus])

HSP 1 Score: 1476.5 bits (3821), Expect = 0.0e+00
Identity = 749/780 (96.03%), Postives = 750/780 (96.15%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE
Sbjct: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
           GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK
Sbjct: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV
Sbjct: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK
Sbjct: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL
Sbjct: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSSGSRGDWKRMFFVLDSQGALYYYKNK     GQQSHSFNRSDEHNN          
Sbjct: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNK-----GQQSHSFNRSDEHNNSMFGRFRARH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN
Sbjct: 361 NRSSSLNEEILRCSTVNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480
           KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE
Sbjct: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480

Query: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540
           IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD
Sbjct: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540

Query: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600
           LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF
Sbjct: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600

Query: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660
           VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE
Sbjct: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660

Query: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720
           PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH
Sbjct: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720

Query: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 775

BLAST of Cucsa.372010.2 vs. NCBI nr
Match: gi|659109433|ref|XP_008454717.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucumis melo])

HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 723/780 (92.69%), Postives = 738/780 (94.62%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           MGAFIKLEDSPMFLKQI SL+RTTD LEDRCRRLH+GCRMFMETLGEASNGDISFADSLE
Sbjct: 1   MGAFIKLEDSPMFLKQIFSLERTTDELEDRCRRLHNGCRMFMETLGEASNGDISFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
           GFGGGQDDPVSVSIGGPVLSKF+TAFRELATYKEQLRSQVEHVLTNRLMQFL+EDLQGVK
Sbjct: 61  GFGGGQDDPVSVSIGGPVLSKFITAFRELATYKEQLRSQVEHVLTNRLMQFLNEDLQGVK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           DSRQRFDKAMH+YDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMN+
Sbjct: 121 DSRQRFDKAMHQYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK
Sbjct: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQAQLDNLQASNSLD SVVSDGIHGSGI+SYRSVETAPQSIVNGKVETIKQGYL
Sbjct: 241 RIQEFRTQAQLDNLQASNSLDPSVVSDGIHGSGISSYRSVETAPQSIVNGKVETIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDE NN          
Sbjct: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDELNNSMFGRFRARH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN
Sbjct: 361 TRSSSLNEEILRCSTVNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480
           KITGAIASLFNSYFLQQP LEAKHPECDKNNFNDYS+ T HEKRLEDGSQVDSVSKILR+
Sbjct: 421 KITGAIASLFNSYFLQQPLLEAKHPECDKNNFNDYSILTLHEKRLEDGSQVDSVSKILRD 480

Query: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540
           IPGNDLCAECGS EPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD
Sbjct: 481 IPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540

Query: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600
           LFRNLGN YCNSIWEGLFLADTGRDN++HA+ATSIPKPSPKDANQLRERYIQAKYVEKQF
Sbjct: 541 LFRNLGNAYCNSIWEGLFLADTGRDNSEHAVATSIPKPSPKDANQLRERYIQAKYVEKQF 600

Query: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660
           VVKDSEAPGNISYAKS+WEAVK NDLREAYRLIAVSAV IVNTTYDNVV VSSSPHLDEE
Sbjct: 601 VVKDSEAPGNISYAKSMWEAVKRNDLREAYRLIAVSAVLIVNTTYDNVVGVSSSPHLDEE 660

Query: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720
            S NQESLNPSSC RDWDSNES +SLQGCSLLHLACQNDNQVMLELLLQFGTDINV DSH
Sbjct: 661 HSVNQESLNPSSCGRDWDSNESRNSLQGCSLLHLACQNDNQVMLELLLQFGTDINVRDSH 720

Query: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 780

BLAST of Cucsa.372010.2 vs. NCBI nr
Match: gi|659109435|ref|XP_008454718.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 [Cucumis melo])

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 718/780 (92.05%), Postives = 733/780 (93.97%), Query Frame = 1

Query: 1   MGAFIKLEDSPMFLKQISSLQRTTDVLEDRCRRLHSGCRMFMETLGEASNGDISFADSLE 60
           MGAFIKLEDSPMFLKQI SL+RTTD LEDRCRRLH+GCRMFMETLGEASNGDISFADSLE
Sbjct: 1   MGAFIKLEDSPMFLKQIFSLERTTDELEDRCRRLHNGCRMFMETLGEASNGDISFADSLE 60

Query: 61  GFGGGQDDPVSVSIGGPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVK 120
           GFGGGQDDPVSVSIGGPVLSKF+TAFRELATYKEQLRSQVEHVLTNRLMQFL+EDLQGVK
Sbjct: 61  GFGGGQDDPVSVSIGGPVLSKFITAFRELATYKEQLRSQVEHVLTNRLMQFLNEDLQGVK 120

Query: 121 DSRQRFDKAMHEYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNV 180
           DSRQRFDKAMH+YDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMN+
Sbjct: 121 DSRQRFDKAMHQYDQAREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNI 180

Query: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240
           EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK
Sbjct: 181 EAKKKYEFLESISALMDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAK 240

Query: 241 RIQEFRTQAQLDNLQASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYL 300
           RIQEFRTQAQLDNLQASNSLD SVVSDGIHGSGI+SYRSVETAPQSIVNGKVETIKQGYL
Sbjct: 241 RIQEFRTQAQLDNLQASNSLDPSVVSDGIHGSGISSYRSVETAPQSIVNGKVETIKQGYL 300

Query: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN---------- 360
           LKRSSGSRGDWKRMFFVLDSQGALYYYKNK     GQQSHSFNRSDE NN          
Sbjct: 301 LKRSSGSRGDWKRMFFVLDSQGALYYYKNK-----GQQSHSFNRSDELNNSMFGRFRARH 360

Query: 361 ---------------INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420
                          +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN
Sbjct: 361 TRSSSLNEEILRCSTVNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWIN 420

Query: 421 KITGAIASLFNSYFLQQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILRE 480
           KITGAIASLFNSYFLQQP LEAKHPECDKNNFNDYS+ T HEKRLEDGSQVDSVSKILR+
Sbjct: 421 KITGAIASLFNSYFLQQPLLEAKHPECDKNNFNDYSILTLHEKRLEDGSQVDSVSKILRD 480

Query: 481 IPGNDLCAECGSSEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540
           IPGNDLCAECGS EPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD
Sbjct: 481 IPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILD 540

Query: 541 LFRNLGNTYCNSIWEGLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQF 600
           LFRNLGN YCNSIWEGLFLADTGRDN++HA+ATSIPKPSPKDANQLRERYIQAKYVEKQF
Sbjct: 541 LFRNLGNAYCNSIWEGLFLADTGRDNSEHAVATSIPKPSPKDANQLRERYIQAKYVEKQF 600

Query: 601 VVKDSEAPGNISYAKSIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEE 660
           VVKDSEAPGNISYAKS+WEAVK NDLREAYRLIAVSAV IVNTTYDNVV VSSSPHLDEE
Sbjct: 601 VVKDSEAPGNISYAKSMWEAVKRNDLREAYRLIAVSAVLIVNTTYDNVVGVSSSPHLDEE 660

Query: 661 PSGNQESLNPSSCTRDWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSH 720
            S NQESLNPSSC RDWDSNES +SLQGCSLLHLACQNDNQVMLELLLQFGTDINV DSH
Sbjct: 661 HSVNQESLNPSSCGRDWDSNESRNSLQGCSLLHLACQNDNQVMLELLLQFGTDINVRDSH 720

Query: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 721 GRTPLHQCISQKNNKLAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 775

BLAST of Cucsa.372010.2 vs. NCBI nr
Match: gi|700194486|gb|KGN49663.1| (hypothetical protein Csa_5G053060 [Cucumis sativus])

HSP 1 Score: 1343.2 bits (3475), Expect = 0.0e+00
Identity = 679/705 (96.31%), Postives = 680/705 (96.45%), Query Frame = 1

Query: 76  GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ 135
           GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ
Sbjct: 34  GPVLSKFVTAFRELATYKEQLRSQVEHVLTNRLMQFLDEDLQGVKDSRQRFDKAMHEYDQ 93

Query: 136 AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL 195
           AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL
Sbjct: 94  AREKFVSLKKNTRGDIVAECEEDLLNSKSAFEKSRFNLVNALMNVEAKKKYEFLESISAL 153

Query: 196 MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ 255
           MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ
Sbjct: 154 MDAHLRYFKLGFDLFGQMEPFIHQVLTYAQQSKELANIEQDKLAKRIQEFRTQAQLDNLQ 213

Query: 256 ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF 315
           ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF
Sbjct: 214 ASNSLDTSVVSDGIHGSGINSYRSVETAPQSIVNGKVETIKQGYLLKRSSGSRGDWKRMF 273

Query: 316 FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN------------------------- 375
           FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNN                         
Sbjct: 274 FVLDSQGALYYYKNKASRYVGQQSHSFNRSDEHNNSMFGRFRARHNRSSSLNEEILRCST 333

Query: 376 INLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL 435
           +NLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL
Sbjct: 334 VNLHTSTIKMDAEDSDLRLCFRIISPSKTFTLQAENEADRMDWINKITGAIASLFNSYFL 393

Query: 436 QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP 495
           QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP
Sbjct: 394 QQPFLEAKHPECDKNNFNDYSVQTRHEKRLEDGSQVDSVSKILREIPGNDLCAECGSSEP 453

Query: 496 EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE 555
           EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE
Sbjct: 454 EWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNTYCNSIWE 513

Query: 556 GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK 615
           GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK
Sbjct: 514 GLFLADTGRDNTQHALATSIPKPSPKDANQLRERYIQAKYVEKQFVVKDSEAPGNISYAK 573

Query: 616 SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR 675
           SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR
Sbjct: 574 SIWEAVKTNDLREAYRLIAVSAVSIVNTTYDNVVSVSSSPHLDEEPSGNQESLNPSSCTR 633

Query: 676 DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK 735
           DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK
Sbjct: 634 DWDSNESSDSLQGCSLLHLACQNDNQVMLELLLQFGTDINVCDSHGRTPLHQCISQKNNK 693

Query: 736 LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 756
           LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE
Sbjct: 694 LAKLLLRRGAKPSIKDCGGLSALERAMEMGAITDEELFLLLTGSE 738