Cucsa.370590.5 (mRNA) Cucumber (Gy14) v1

NameCucsa.370590.5
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionAscorbate peroxidase
Locationscaffold03678 : 282598 .. 287539 (+)
Sequence length1191
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCCTTTCTCTTTATACTAACTACAATTTCCATTCCTCCATCAATAATTCTTCATCTTCTTCCTTAGCTAATCTCTCTTCTCTACGAATCAAACGCCCTCCCTTTCGGTCCTCTCTACCCTCCTCCCATGGCCTTGCCCGTCGTAGACACTGAATACCTCAAGGAGATCGACAAGGCTCGCCGTGACCTTCGCGCTCTCATTGCTAATCGGAATTGTGCTCCCATCATGCTTCGTTTGGCGTATGCTCTCTTTACATTGACTTTCATTGACTATTTTCAATTATTATCACGAATACTCGTTTTATATCTCTTGGATTGTTATTTCATTTCTCTATCCACCTCGGTTGATTGAATGAAAATAGACGGACAGTTGACTTTTCTTCATTTAGTTTGTTGTTGTTGATTTCTGCTGTGTTTGTTTTTTTTTTttGCTGGAATTGCATGGATGAGATTATTAGGAATTCTTTCATTTCGCTGTTTTTCTCATTTCTGTTGAATGAAGCTTTGCTTTCTCTTTGATTTTGATGATGGATGATTGTAGCAATTTTGTTTTCTAATGTTTCAAGCACTGTTCTGTCTTTTGTAGTTGGCATGACGCTGGCACTTACGATGTGGTTACGAAAATTGGTGGACCTAATGGATCGATTAGGAACGAGGAGGAGTTTTCTCATGGTTCTAATAATGGACTGAAAAAAGCTATTGATTTTTGTGGTATGCTTTTTTtACTCCTTTACTTTTTtCTTCTTTGCGTTGTGGGTCATGCAAACCGAAAGTCAACCTAATAATTTATCTGAGTAACGTTCCAAGTATCCCCACTGTTGTTAGATTCAAAATTACAATGGTGTCGTTTCAAGATTTTTTCTTATTTATCGTCAATGTTCACTAAGTATTGCAAATTAACATATTTGACTTGCACTTCAAGTATAGGAAATTCTACAGTGTTTTTGTCTCTCCTAATGATTGTTGACAACTGATATTTCTTGATGTTGGTGATTGCTTCTTTCTTTAATAACAGAGGAAGTGAAGTCTAAACATCCGAAGATTACTTATGCAGACCTGTACCAGGTAATTGCAACTGTTATGGTACTATTGATATGCCTGCATTTTTGTAATTCTACGACGCTTGGTTTAACCTTATTTTATGTGGAATTGAATGGAATCTGCATTAAAGGAATCACATGCGTTTTAAATCTTAGCATGAGAAATATGTTTTTTTtATCAACAATATATAAATTGAGATTAGGTGAACAGGTCTACCGTTTTtGTTAATGGGGTTGTATATATGTTATAGAAGATTATGCTGCTTCAGCGTGTTTGAAGTTTGCTTTTTTGTTAATCTTAACGTGAGAAATATGTTGTCATTGAAATTTTATTAGCATAGTTGTACGTACTGTGATTTTTTTtATTtCTTTATAGTGGAAAAGATGCATGCGTTCATATGCTATATGTGCCTAAGTTTGTATTTTAAGAATCAATTTTACATATACATTTTCATAACTCCTAGATAAAAGCTTGATAAAAAGTGCAAATTGTAATAGGAAGATTGTATAACACTGACAAAGTCGTTGAATTAACTTTAAAAGTATAAATGTCATGTACTGTAATCAGTATTGTTGAATCTTTTTGGCAATGGTAAACTATTTTGTTATAAGTTGCATTCGGAGTTGGGGAGAAATTGGCCATAAACTCTGATCACATTTGCTCCCTATCTCACTCCAAAATAAAAATGGGATGCTATTTTCATATATTTGTTCCTATAGCCTCCCTGACCAAAAGGTGGTGCCTGTCATGGAGCAGTACCTAGTGACGATGTGAACTTTAGGCAAATGCAGATACTAGAGTTTCAGAAAATCACTTTTACCTGATCTTAGCATCAATAAATTTTTAATTATTATTTACCAAGGCATACTTAGAGATTGTTATCCATTACAATGCATCTACTATGGCAAGAACAATGAGATTATCAAGTTTTCTCAATTCATCAATGCTATAGAGCTTGTACAATTGACTAAGGTCTGATAGTGTTTAAAATGTACTCAATTCAGCTAGCTGGCGTTGTTGCTGTTGAGGTCACTGGAGGCCCCACCATTGACTTTGTTCCCGGTAGAAAGGTACAGTAATTTATTTGTTTACTTTGTGAATGTCAGCTGCTGGTTATATTATTTGTTCCAGTAATAACTAAATCCTTTGTTCTCAGGATTCAAACATTTGTCCTAAGGAAGGCCGACTTCCTGATGCTAAAAAaGGTATAATAATTCCTTTATCATCTTGTACGTAGTTTCCTGCAGTCCTGCAAAGTGAATGATATCTGAATATCTTCTCTGCATCTAGTGTGAATAAAACTATTATGGTTGAGTTTCGAGCTAACTGGTTTATTTTATTTTATAATCATTTGTCAAAGAGAGAAAAGGAAGATCTGCAATTGTTTATCGTGTTTTAATTAGTTGCACAATTTTACATAAAATTGTGGCTCTTGTGTTTAAGATGAGAGTAAAGTAAGTCGTAGATGTTTGGTCGGCTTAGAGGTTCTATATGGAATCGTAAGGTGCCAGTGCCGCAACCTAGAATTTGATTTAATCATGTTGATATTGTTAATACCATCGCAGTATATGCCAATTTTTTAAAAGTTtAAAAGGCAGCCTTGAAGCTTTTCCTTTTCAAGAGGGAGGCGTAAGCCTCAAGGTGGTATGAAGCATAAGCCTCAAATAATGAATTAAATACACTTTACAAAACCTAAATTGGTATAATCAAGTATTGCTAAACATGATAATCATATAAAAGTGTAGAAAGAAACATCTCCCGCATAATCTTCACTTAATACTAAATTTTAAAAGGACATCTTCAACATCATTAATAATGCTATTATTAGTAATAGGATTTATTTATACTATATATGTCTGCTCTCAGCATTCTTAAGGTCACAGCAATAAAAATAGTCCTAGTTCAAATTGTCACAACTCAAACCTTACTATTTCTTAAAAaTTAGGCAAATCACTATTATTTTTCTAATCACTAGGCGAGACATAGAGGTCTTAACCCTAAGTGCCTTTCAGAAAGGTGTAAGCCTCATATGATTCAACAAAGCTGTAAGTCTCAAGGCTAAAACGTAGTGTCTTACCTCGAGGCAAGTCTCAAGGCGTAAACCTCAATAGTTTTTTAAAACGTCCTATATGTTTTTCGACATGATAATTCATGTTTAGACTGCTTTTTAATAAAATATTTTGTCCTCGTTATTTGTACTTCACTGGAAGTTGCTTCTTATTTTCTCCCGGGGTTTTATGATAAAGATACGTTCAAATTCTATAAGTTTTGTGCATTTTTGCTGTCGTGCTCAAGTTTTGTGCATTTTTGCTGTCGTGCTCAAGTTTTGTTATGAAATTGTCACTTATCAAATTTGATTTCTTAAATGTTAGCCTTTTTCTAATTTTGGTAATAGGTGCACCGCATCTGCGTGACATTTTTTATAGGATGGGCCTGTCTGACAAGGATATTGTTGCATTATCCGGGGGTCACACTTTGGTAATTTCTTCACACATCTTATTCTTGCATTGGTTTATTTTTGTATTACTAATTGAATCATATGTTGTGAATTCTGATGTTATAGGGAAGAGCACATCCGGAGAGATCTGGTTTTGATGGACCCTGGACAGAGGATCCTTTGAAGTTTGATAACTCATACTTTGTGTAGGACTTGAGCTACATCTCAGACTAATCCCTGTATCATTTTAATAGGCCATGCATAATGTTATTTTTAATTGTTTGCAATTTGATGTTGATAGGGAACTGTTGAAAGGAGAAGCAGAGGGATTATTAAAACTTCCTACGGACAAGGCTTTACTTGAAGATCCTGAGTTCCGCCAATATGTGGAATTGTATGCCAAAGTAAGGTCTTTTATGCATTATGGAAAAGCGGGAAAATGTGCTAGTTTTCCATCATAAGAGGAAATTAAAAGTTCTGTAGTCTAATTAATTCAATCTTTAAATAAAGAATCGGCTTGGTTTGGACTTTGAAGTGACCTAAATAGAGGAACTAGTTAAAAGAATACCACCAATTAGAACTTTAGAACTGGCCGGTGTGATTAGTTTCTCAGAAGGTCATGGGAGTGTGGATTTTTTTAGTACAACTGTGGGTAGGGGATTAGGTCACAGAATCGGTCACAGAATCGTGCCTTTCATTTTTTTtGTAAAACTGCAGGGTGGAGTATTGAGCCTCTGACCTCTAGAGAGTCAGAGACTGAACTCATGTCAATACCAATGAGCGATCATGTATTTGTTGAAAACTTGGTAGGCTATATGGGTCTTTAGAATTAATGATTATGATTATGGTACATTGTATCGGTCACATTTATGACATGACAGATTTTAAAGAAACGATCCAACAATTAAATGATAGTTCTAGTACTAATATAATTGATAACTGGAAATTAAGATCCATTTGAAATTTATGCCATAGTTTAAGGAAGTAATGAATATGACTAAGCCATTCTTCTATTGAGTTACCATATATATTGAGTTATTTCTTATTATGTTTTTGCCAATTTGTACAGGATGAAGATGCATTCTTCAAAGATTATGCTGCATCACACAAGAAACTTTCGGAGCTTGGGTTCACTCCAGGTTCTGCTAAGGCGATTGTGAAAGACAGCATCGTATTAGCACAGGGTGCTGTTGGAGTTGCGGTTGCAGCTGCTGTGGTAATTCTGAGCTACTTATATGAAGTCCGCAAAAAATCGAAGTAACTATGGTAGCAAGGTCGACTAGTTGTCTATATAGCATTCACTATTGAGTTCATTTCTAGACCTGGAAAGTTATGCACTTGTGATCTGATACTTTATATACCTGATTCTATGTATTAGCCTTATAAACACAATCACTCTGCAAGACTATTATCATAACATAAAAAaTTTATGTCTTGCTTTATATGTTTAATCCGTTGTGAAA

mRNA sequence

TCTCCTTTCTCTTTATACTAACTACAATTTCCATTCCTCCATCAATAATTCTTCATCTTCTTCCTTAGCTAATCTCTCTTCTCTACGAATCAAACGCCCTCCCTTTCGGTCCTCTCTACCCTCCTCCCATGGCCTTGCCCGTCGTAGACACTGAATACCTCAAGGAGATCGACAAGGCTCGCCGTGACCTTCGCGCTCTCATTGCTAATCGGAATTGTGCTCCCATCATGCTTCGTTTGGCTTGGCATGACGCTGGCACTTACGATGTGGTTACGAAAATTGGTGGACCTAATGGATCGATTAGGAACGAGGAGGAGTTTTCTCATGGTTCTAATAATGGACTGAAAAAAGCTATTGATTTTTGTGAGGAAGTGAAGTCTAAACATCCGAAGATTACTTATGCAGACCTGTACCAGCTAGCTGGCGTTGTTGCTGTTGAGGTCACTGGAGGCCCCACCATTGACTTTGTTCCCGGTAGAAAGGATTCAAACATTTGTCCTAAGGAAGGCCGACTTCCTGATGCTAAAAAAGGTGCACCGCATCTGCGTGACATTTTTTATAGGATGGGCCTGTCTGACAAGGATATTGTTGCATTATCCGGGGGTCACACTTTGGGAAGAGCACATCCGGAGAGATCTGGTTTTGATGGACCCTGGACAGAGGATCCTTTGAAGTTTGATAACTCATACTTTGTGGAACTGTTGAAAGGAGAAGCAGAGGGATTATTAAAACTTCCTACGGACAAGGCTTTACTTGAAGATCCTGAGTTCCGCCAATATGTGGAATTGTATGCCAAAGATGAAGATGCATTCTTCAAAGATTATGCTGCATCACACAAGAAACTTTCGGAGCTTGGGTTCACTCCAGGTTCTGCTAAGGCGATTGTGAAAGACAGCATCGTATTAGCACAGGGTGCTGTTGGAGTTGCGGTTGCAGCTGCTGTGGTAATTCTGAGCTACTTATATGAAGTCCGCAAAAAATCGAAGTAACTATGGTAGCAAGGTCGACTAGTTGTCTATATAGCATTCACTATTGAGTTCATTTCTAGACCTGGAAAGTTATGCACTTGTGATCTGATACTTTATATACCTGATTCTATGTATTAGCCTTATAAACACAATCACTCTGCAAGACTATTATCATAACATAAAAAATTTATGTCTTGCTTTATATGTTTAATCCGTTGTGAAA

Coding sequence (CDS)

ATGGCCTTGCCCGTCGTAGACACTGAATACCTCAAGGAGATCGACAAGGCTCGCCGTGACCTTCGCGCTCTCATTGCTAATCGGAATTGTGCTCCCATCATGCTTCGTTTGGCTTGGCATGACGCTGGCACTTACGATGTGGTTACGAAAATTGGTGGACCTAATGGATCGATTAGGAACGAGGAGGAGTTTTCTCATGGTTCTAATAATGGACTGAAAAAAGCTATTGATTTTTGTGAGGAAGTGAAGTCTAAACATCCGAAGATTACTTATGCAGACCTGTACCAGCTAGCTGGCGTTGTTGCTGTTGAGGTCACTGGAGGCCCCACCATTGACTTTGTTCCCGGTAGAAAGGATTCAAACATTTGTCCTAAGGAAGGCCGACTTCCTGATGCTAAAAAaGGTGCACCGCATCTGCGTGACATTTTTTATAGGATGGGCCTGTCTGACAAGGATATTGTTGCATTATCCGGGGGTCACACTTTGGGAAGAGCACATCCGGAGAGATCTGGTTTTGATGGACCCTGGACAGAGGATCCTTTGAAGTTTGATAACTCATACTTTGTGGAACTGTTGAAAGGAGAAGCAGAGGGATTATTAAAACTTCCTACGGACAAGGCTTTACTTGAAGATCCTGAGTTCCGCCAATATGTGGAATTGTATGCCAAAGATGAAGATGCATTCTTCAAAGATTATGCTGCATCACACAAGAAACTTTCGGAGCTTGGGTTCACTCCAGGTTCTGCTAAGGCGATTGTGAAAGACAGCATCGTATTAGCACAGGGTGCTGTTGGAGTTGCGGTTGCAGCTGCTGTGGTAATTCTGAGCTACTTATATGAAGTCCGCAAAAAATCGAAGTAA

Protein sequence

MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNEEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSNICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPLKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK*
BLAST of Cucsa.370590.5 vs. Swiss-Prot
Match: APX3_ARATH (L-ascorbate peroxidase 3, peroxisomal OS=Arabidopsis thaliana GN=APX3 PE=1 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.1e-134
Identity = 234/287 (81.53%), Postives = 255/287 (88.85%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA P+VD EYLKEI KARR+LR+LIAN+NCAPIMLRLAWHDAGTYD  +K GGPNGSIRN
Sbjct: 1   MAAPIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEE +HG+N+GLK A+D CE VK+KHPKITYADLYQLAGVVAVEVTGGP I FVPGRKDS
Sbjct: 61  EEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           N+CPKEGRLPDAK+G  HLRD+FYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT++P
Sbjct: 121 NVCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE+EGLLKLPTDK LLEDPEFR+ VELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTP-GSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGF P  SA   V DS +LAQ A GVAVAAAVV   Y YE+RK+ K
Sbjct: 241 ELGFNPNSSAGKAVADSTILAQSAFGVAVAAAVVAFGYFYEIRKRMK 287

BLAST of Cucsa.370590.5 vs. Swiss-Prot
Match: APX4_ORYSJ (Probable L-ascorbate peroxidase 4 OS=Oryza sativa subsp. japonica GN=APX4 PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 1.6e-130
Identity = 228/291 (78.35%), Postives = 257/291 (88.32%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVD EYL+++D+ARR LRALI+++ CAPIMLRLAWHDAGTYDV TK GG NGSIR 
Sbjct: 1   MAAPVVDAEYLRQVDRARRHLRALISSKGCAPIMLRLAWHDAGTYDVNTKTGGANGSIRY 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEE++HGSN GLK AID  E +K+K PKITYADLYQLAGVVAVEVTGGPT++F+PGR+DS
Sbjct: 61  EEEYTHGSNAGLKIAIDLLEPIKAKSPKITYADLYQLAGVVAVEVTGGPTVEFIPGRRDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           ++CP+EGRLPDAKKGA HLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGF+G WT++P
Sbjct: 121 SVCPREGRLPDAKKGALHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFEGAWTQEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYF+ELLKGE+EGLLKLPTDKALLEDP FR+YV+LYA+DED FFKDYA SHKKLS
Sbjct: 181 LKFDNSYFLELLKGESEGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHKKLS 240

Query: 241 ELGFT-----PGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFT     P S K+ +    VLAQ AVGVAVAAAVVI+SYLYE  KKSK
Sbjct: 241 ELGFTPRSSGPASTKSDLSTGAVLAQSAVGVAVAAAVVIVSYLYEASKKSK 291

BLAST of Cucsa.370590.5 vs. Swiss-Prot
Match: APX3_ORYSJ (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. japonica GN=APX3 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-120
Identity = 214/287 (74.56%), Postives = 244/287 (85.02%), Query Frame = 1

Query: 2   ALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNE 61
           A PVVD EY+ E+++ARRDLRALIA+++CAPIMLRLAWHDAGTYD  TK GGPNGSIR  
Sbjct: 3   AAPVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFP 62

Query: 62  EEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSN 121
           +E+SH +N G+K AID  E +K KHPKITYADLYQLAGVVAVEVTGGPTID+VPGR+DS+
Sbjct: 63  QEYSHAANAGIKIAIDLLEPMKQKHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSS 122

Query: 122 ICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPL 181
             P+EGRLPDAKKGA HLR++FYRMGLSDKDIVALSGGHTLG+A PERSGFDG WT+DPL
Sbjct: 123 DSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPL 182

Query: 182 KFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSE 241
           KFDNSYF+ELLK  +EGLLKLPTDKAL+EDP FR+YVELYAKDEDAFF+DYA SHKKLSE
Sbjct: 183 KFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSE 242

Query: 242 LGFTPGSA----KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKK 285
           LGFTP  +    K+  K   +L Q A GVAVAAAVV  +YL E  K+
Sbjct: 243 LGFTPPRSAFIYKSCQKPKSLLMQTAAGVAVAAAVVAWAYLCESNKR 289

BLAST of Cucsa.370590.5 vs. Swiss-Prot
Match: APX3_ORYSI (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica GN=APX3 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 2.6e-120
Identity = 213/287 (74.22%), Postives = 244/287 (85.02%), Query Frame = 1

Query: 2   ALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNE 61
           A PVVD EY+ E+++ARRDLRALIA+++CAPIMLRLAWHDAGTYD  TK GGPNGSIR  
Sbjct: 3   AAPVVDAEYMAEVERARRDLRALIASKSCAPIMLRLAWHDAGTYDKATKTGGPNGSIRFP 62

Query: 62  EEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSN 121
           +E+SH +N G+K AID  E +K +HPKITYADLYQLAGVVAVEVTGGPTID+VPGR+DS+
Sbjct: 63  QEYSHAANAGIKIAIDLLEPMKQRHPKITYADLYQLAGVVAVEVTGGPTIDYVPGRRDSS 122

Query: 122 ICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPL 181
             P+EGRLPDAKKGA HLR++FYRMGLSDKDIVALSGGHTLG+A PERSGFDG WT+DPL
Sbjct: 123 DSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWTKDPL 182

Query: 182 KFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSE 241
           KFDNSYF+ELLK  +EGLLKLPTDKAL+EDP FR+YVELYAKDEDAFF+DYA SHKKLSE
Sbjct: 183 KFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHKKLSE 242

Query: 242 LGFTPGSA----KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKK 285
           LGFTP  +    K+  K   +L Q A GVAVAAAVV  +YL E  K+
Sbjct: 243 LGFTPPRSAFIYKSCQKPKSLLMQTAAGVAVAAAVVAWAYLCESNKR 289

BLAST of Cucsa.370590.5 vs. Swiss-Prot
Match: APX5_ARATH (L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana GN=APX5 PE=1 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 7.0e-105
Identity = 190/281 (67.62%), Postives = 222/281 (79.00%), Query Frame = 1

Query: 6   VDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNEEEFS 65
           VD EYLKEI+K RRDLRALI++RNCAPIMLRLAWHDAGTYD   K GG NGSIR +EE +
Sbjct: 5   VDAEYLKEIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELN 64

Query: 66  HGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSNICPK 125
              N GL+KA+ FCEEVK+KHP+++YADLYQLAGVVAVEVTGGP I F PGRKD++    
Sbjct: 65  RPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSAD- 124

Query: 126 EGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPLKFDN 185
           +G LP+  +GA HLR +F RMGL D+DIVALSGGHTLGRAH ERS F+GPWT+DPLKFDN
Sbjct: 125 DGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDN 184

Query: 186 SYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSELGFT 245
           SYFVELLKGE  GLL+L TDKALL+DP+F  +V+LYAKDED FFK YA SHKKLSELGF 
Sbjct: 185 SYFVELLKGETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELGFN 244

Query: 246 PGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           P       +    + Q  +G+AVAAAVVI +  YE  ++ K
Sbjct: 245 PPR-----RIPSAVTQQTLGIAVAAAVVIFTICYEASRRGK 279

BLAST of Cucsa.370590.5 vs. TrEMBL
Match: Q948P1_9ROSI (Peroxisomal ascorbate peroxidase OS=Cucurbita cv. Kurokawa Amakuri GN=pAPX PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 1.7e-150
Identity = 266/286 (93.01%), Postives = 273/286 (95.45%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MALPVVDTEYLKEI+KARRDLRALIANRNCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MALPVVDTEYLKEIEKARRDLRALIANRNCAPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           +EE+SHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFV GRKDS
Sbjct: 61  QEEYSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVSGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
            I P+EGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP
Sbjct: 121 RISPREGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE+EGLLKLPTDKALLEDPEFR YVELYAKDEDAFFKDYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGESEGLLKLPTDKALLEDPEFRPYVELYAKDEDAFFKDYAESHKKLS 240

Query: 241 ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTPGSA+AI  DS VLAQGAVGVAVAAAVVILSY YE+RK  K
Sbjct: 241 ELGFTPGSARAIANDSTVLAQGAVGVAVAAAVVILSYFYEIRKNLK 286

BLAST of Cucsa.370590.5 vs. TrEMBL
Match: Q39780_GOSHI (Ascorbate peroxidase OS=Gossypium hirsutum GN=gAPX PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.0e-146
Identity = 259/288 (89.93%), Postives = 274/288 (95.14%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVDTEYLKEIDKARRDLRALIA +NCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MAFPVVDTEYLKEIDKARRDLRALIALKNCAPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEF+HG+N+GLK AIDFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFTHGANSGLKIAIDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICP+EGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 121 NICPREGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTNEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYF+ELLKGE+EGLLKLPTDKALL+DPEFR+YVELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFLELLKGESEGLLKLPTDKALLDDPEFRKYVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTPGSA--KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTP SA  K +VKDS VLAQGAVGVAVAAAVVILSY YEVRK+ K
Sbjct: 241 ELGFTPTSARSKVMVKDSTVLAQGAVGVAVAAAVVILSYFYEVRKRMK 288

BLAST of Cucsa.370590.5 vs. TrEMBL
Match: A0A0B0MPD9_GOSAR (L-ascorbate peroxidase 3, peroxisomal-like protein OS=Gossypium arboreum GN=F383_00644 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 4.4e-146
Identity = 258/288 (89.58%), Postives = 274/288 (95.14%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVDTEYLKEIDKARRDLRALIA +NC+PIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MAFPVVDTEYLKEIDKARRDLRALIALKNCSPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEF+HG+N+GLK AIDFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFTHGANSGLKIAIDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICP+EGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 121 NICPREGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTNEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYF+ELLKGE+EGLLKLPTDKALL+DPEFR+YVELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFLELLKGESEGLLKLPTDKALLDDPEFRKYVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTPGSA--KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTP SA  K +VKDS VLAQGAVGVAVAAAVVILSY YEVRK+ K
Sbjct: 241 ELGFTPTSARSKVMVKDSTVLAQGAVGVAVAAAVVILSYFYEVRKRMK 288

BLAST of Cucsa.370590.5 vs. TrEMBL
Match: A0A0D2SN99_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G254100 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 7.4e-146
Identity = 258/288 (89.58%), Postives = 272/288 (94.44%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVDTEYLKEIDKARRDLRA IA +NCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MAFPVVDTEYLKEIDKARRDLRAFIALKNCAPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEF+HG+N+GLK AIDFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFTHGANSGLKIAIDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICP+EGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 121 NICPREGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTNEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE+EGLLKLPTDKAL +DPEFR+YVELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGESEGLLKLPTDKALFDDPEFRKYVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTPGSA--KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTP SA  K +VKDS VLAQGAVGVAVAAAVVILSY YEVRK+ K
Sbjct: 241 ELGFTPTSARSKVMVKDSTVLAQGAVGVAVAAAVVILSYFYEVRKRMK 288

BLAST of Cucsa.370590.5 vs. TrEMBL
Match: A0A0K2SSH7_CITUN (Ascorbate peroxidase (Fragment) OS=Citrus unshiu GN=CitAPX3 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.2e-143
Identity = 253/287 (88.15%), Postives = 269/287 (93.73%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MALPVVDTEYLKEIDKARRDLRALIA +NCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 3   MALPVVDTEYLKEIDKARRDLRALIAYKNCAPIMLRLAWHDAGTYDVNTKTGGPNGSIRN 62

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEE+SHGSNNGLK A+DFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPT+DFVPGRKDS
Sbjct: 63  EEEYSHGSNNGLKIALDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTVDFVPGRKDS 122

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
            I PKEGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 123 KISPKEGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTREP 182

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELL GE+EGLL+LPTDKALLEDPEFR+YVELYAKDEDAFF+DYAASHKKLS
Sbjct: 183 LKFDNSYFVELLNGESEGLLQLPTDKALLEDPEFRRYVELYAKDEDAFFRDYAASHKKLS 242

Query: 241 ELGFTPGS-AKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGF+PGS +K+   D  +LAQGAVGVAV  AVVILSY YEVRK+ K
Sbjct: 243 ELGFSPGSGSKSTANDCTILAQGAVGVAVTVAVVILSYFYEVRKRMK 289

BLAST of Cucsa.370590.5 vs. TAIR10
Match: AT4G35000.1 (AT4G35000.1 ascorbate peroxidase 3)

HSP 1 Score: 480.7 bits (1236), Expect = 6.2e-136
Identity = 234/287 (81.53%), Postives = 255/287 (88.85%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA P+VD EYLKEI KARR+LR+LIAN+NCAPIMLRLAWHDAGTYD  +K GGPNGSIRN
Sbjct: 1   MAAPIVDAEYLKEITKARRELRSLIANKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEE +HG+N+GLK A+D CE VK+KHPKITYADLYQLAGVVAVEVTGGP I FVPGRKDS
Sbjct: 61  EEEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           N+CPKEGRLPDAK+G  HLRD+FYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT++P
Sbjct: 121 NVCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE+EGLLKLPTDK LLEDPEFR+ VELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTP-GSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGF P  SA   V DS +LAQ A GVAVAAAVV   Y YE+RK+ K
Sbjct: 241 ELGFNPNSSAGKAVADSTILAQSAFGVAVAAAVVAFGYFYEIRKRMK 287

BLAST of Cucsa.370590.5 vs. TAIR10
Match: AT4G35970.1 (AT4G35970.1 ascorbate peroxidase 5)

HSP 1 Score: 381.7 bits (979), Expect = 3.9e-106
Identity = 190/281 (67.62%), Postives = 222/281 (79.00%), Query Frame = 1

Query: 6   VDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNEEEFS 65
           VD EYLKEI+K RRDLRALI++RNCAPIMLRLAWHDAGTYD   K GG NGSIR +EE +
Sbjct: 5   VDAEYLKEIEKTRRDLRALISSRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEELN 64

Query: 66  HGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSNICPK 125
              N GL+KA+ FCEEVK+KHP+++YADLYQLAGVVAVEVTGGP I F PGRKD++    
Sbjct: 65  RPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSAD- 124

Query: 126 EGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPLKFDN 185
           +G LP+  +GA HLR +F RMGL D+DIVALSGGHTLGRAH ERS F+GPWT+DPLKFDN
Sbjct: 125 DGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFDN 184

Query: 186 SYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSELGFT 245
           SYFVELLKGE  GLL+L TDKALL+DP+F  +V+LYAKDED FFK YA SHKKLSELGF 
Sbjct: 185 SYFVELLKGETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELGFN 244

Query: 246 PGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           P       +    + Q  +G+AVAAAVVI +  YE  ++ K
Sbjct: 245 PPR-----RIPSAVTQQTLGIAVAAAVVIFTICYEASRRGK 279

BLAST of Cucsa.370590.5 vs. TAIR10
Match: AT3G09640.1 (AT3G09640.1 ascorbate peroxidase 2)

HSP 1 Score: 318.9 bits (816), Expect = 3.1e-87
Identity = 155/241 (64.32%), Postives = 183/241 (75.93%), Query Frame = 1

Query: 4   PVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNEEE 63
           P V  EY K + + +R LR LIA ++CAPI+LRLAWH AGT+DV TK GGP G+IR+ +E
Sbjct: 7   PEVKEEYKKAVQRCKRKLRGLIAEKHCAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHPQE 66

Query: 64  FSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSNIC 123
            +H +NNGL  A+   + +K   P ++YAD YQLAGVVAVE+TGGP I F PGR D    
Sbjct: 67  LAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKVEP 126

Query: 124 PKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPLKF 183
           P EGRLP A KG  HLRD+F RMGL+DKDIVALSGGHTLGR H ERSGF+G WT +PL F
Sbjct: 127 PPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPLIF 186

Query: 184 DNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSELG 243
           DNSYF E+L GE EGLL+LPTDKALL+DP F  +VE YA DEDAFF+DY  +H KLSELG
Sbjct: 187 DNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSELG 246

Query: 244 F 245
           F
Sbjct: 247 F 247

BLAST of Cucsa.370590.5 vs. TAIR10
Match: AT1G07890.3 (AT1G07890.3 ascorbate peroxidase 1)

HSP 1 Score: 308.9 bits (790), Expect = 3.2e-84
Identity = 151/242 (62.40%), Postives = 184/242 (76.03%), Query Frame = 1

Query: 4   PVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRNEEE 63
           P V  +Y K ++K RR LR LIA +NCAPIM+RLAWH AGT+D  ++ GGP G++R + E
Sbjct: 6   PTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTMRFDAE 65

Query: 64  FSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSNIC 123
            +HG+N+G+  A+   + ++ + P I++AD +QLAGVVAVEVTGGP I F PGR+D    
Sbjct: 66  QAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGREDKPQP 125

Query: 124 PKEGRLPDAKKGAPHLRDIFYR-MGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDPLK 183
           P EGRLPDA KG  HLRD+F + MGLSDKDIVALSG HTLGR H +RSGF+G WT +PL 
Sbjct: 126 PPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWTSNPLI 185

Query: 184 FDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLSEL 243
           FDNSYF ELL GE EGLL+L +DKALL+DP FR  VE YA DEDAFF DYA +H KLSEL
Sbjct: 186 FDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKLSEL 245

Query: 244 GF 245
           GF
Sbjct: 246 GF 247

BLAST of Cucsa.370590.5 vs. TAIR10
Match: AT4G08390.1 (AT4G08390.1 stromal ascorbate peroxidase)

HSP 1 Score: 243.4 bits (620), Expect = 1.7e-64
Identity = 128/256 (50.00%), Postives = 165/256 (64.45%), Query Frame = 1

Query: 13  EIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKI----GGPNGSIRNEEEFSHGS 72
           ++  AR D++ L++ + C PI++RL WHDAGTY+   K     GG NGS+R + E  H +
Sbjct: 106 QLKNAREDIKELLSTKFCHPILVRLGWHDAGTYNKNIKEWPQRGGANGSLRFDIELKHAA 165

Query: 73  NNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDSN---ICPK 132
           N GL  A++  +++K K+  I+YADL+QLA   A+E  GGP I    GR D++    CP+
Sbjct: 166 NAGLVNALNLIKDIKEKYSGISYADLFQLASATAIEEAGGPKIPMKYGRVDASGPEDCPE 225

Query: 133 EGRLPDAKKGAP--HLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGP-------- 192
           EGRLPDA   +P  HLR++FYRMGL DKDIVALSG HTLGR+ PERSG+  P        
Sbjct: 226 EGRLPDAGPPSPATHLREVFYRMGLDDKDIVALSGAHTLGRSRPERSGWGKPETKYTKEG 285

Query: 193 --------WTEDPLKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDA 244
                   WT + LKFDNSYF E+ +   E LL LPTD A+ ED  F+ Y E YA D+DA
Sbjct: 286 PGAPGGQSWTPEWLKFDNSYFKEIKEKRDEDLLVLPTDAAIFEDSSFKVYAEKYAADQDA 345

BLAST of Cucsa.370590.5 vs. NCBI nr
Match: gi|778722949|ref|XP_004144841.2| (PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis sativus])

HSP 1 Score: 575.1 bits (1481), Expect = 6.9e-161
Identity = 285/286 (99.65%), Postives = 286/286 (100.00%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN
Sbjct: 42  MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 101

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 102 EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 161

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICPKEG+LPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP
Sbjct: 162 NICPKEGQLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 221

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS
Sbjct: 222 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 281

Query: 241 ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK
Sbjct: 282 ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 327

BLAST of Cucsa.370590.5 vs. NCBI nr
Match: gi|659094254|ref|XP_008447964.1| (PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis melo])

HSP 1 Score: 570.5 bits (1469), Expect = 1.7e-159
Identity = 282/286 (98.60%), Postives = 283/286 (98.95%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN
Sbjct: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICPKEG LPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP
Sbjct: 121 NICPKEGHLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE EGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGETEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAESHKKLS 240

Query: 241 ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTPGSAKAIVKDS+VLAQGAVGVAVAAAVVILSYLYEVRKKSK
Sbjct: 241 ELGFTPGSAKAIVKDSLVLAQGAVGVAVAAAVVILSYLYEVRKKSK 286

BLAST of Cucsa.370590.5 vs. NCBI nr
Match: gi|15430584|dbj|BAB64351.1| (peroxisomal ascorbate peroxidase [Cucurbita cv. Kurokawa Amakuri])

HSP 1 Score: 540.0 bits (1390), Expect = 2.4e-150
Identity = 266/286 (93.01%), Postives = 273/286 (95.45%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MALPVVDTEYLKEI+KARRDLRALIANRNCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MALPVVDTEYLKEIEKARRDLRALIANRNCAPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           +EE+SHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFV GRKDS
Sbjct: 61  QEEYSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVSGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
            I P+EGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP
Sbjct: 121 RISPREGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYFVELLKGE+EGLLKLPTDKALLEDPEFR YVELYAKDEDAFFKDYA SHKKLS
Sbjct: 181 LKFDNSYFVELLKGESEGLLKLPTDKALLEDPEFRPYVELYAKDEDAFFKDYAESHKKLS 240

Query: 241 ELGFTPGSAKAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTPGSA+AI  DS VLAQGAVGVAVAAAVVILSY YE+RK  K
Sbjct: 241 ELGFTPGSARAIANDSTVLAQGAVGVAVAAAVVILSYFYEIRKNLK 286

BLAST of Cucsa.370590.5 vs. NCBI nr
Match: gi|1019946|gb|AAB52954.1| (ascorbate peroxidase [Gossypium hirsutum])

HSP 1 Score: 526.6 bits (1355), Expect = 2.8e-146
Identity = 259/288 (89.93%), Postives = 274/288 (95.14%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVDTEYLKEIDKARRDLRALIA +NCAPIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MAFPVVDTEYLKEIDKARRDLRALIALKNCAPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEF+HG+N+GLK AIDFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFTHGANSGLKIAIDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICP+EGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 121 NICPREGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTNEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYF+ELLKGE+EGLLKLPTDKALL+DPEFR+YVELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFLELLKGESEGLLKLPTDKALLDDPEFRKYVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTPGSA--KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTP SA  K +VKDS VLAQGAVGVAVAAAVVILSY YEVRK+ K
Sbjct: 241 ELGFTPTSARSKVMVKDSTVLAQGAVGVAVAAAVVILSYFYEVRKRMK 288

BLAST of Cucsa.370590.5 vs. NCBI nr
Match: gi|728813799|gb|KHG01334.1| (L-ascorbate peroxidase 3, peroxisomal -like protein [Gossypium arboreum])

HSP 1 Score: 525.4 bits (1352), Expect = 6.2e-146
Identity = 258/288 (89.58%), Postives = 274/288 (95.14%), Query Frame = 1

Query: 1   MALPVVDTEYLKEIDKARRDLRALIANRNCAPIMLRLAWHDAGTYDVVTKIGGPNGSIRN 60
           MA PVVDTEYLKEIDKARRDLRALIA +NC+PIMLRLAWHDAGTYDV TK GGPNGSIRN
Sbjct: 1   MAFPVVDTEYLKEIDKARRDLRALIALKNCSPIMLRLAWHDAGTYDVSTKTGGPNGSIRN 60

Query: 61  EEEFSHGSNNGLKKAIDFCEEVKSKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120
           EEEF+HG+N+GLK AIDFCEEVK+KHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS
Sbjct: 61  EEEFTHGANSGLKIAIDFCEEVKAKHPKITYADLYQLAGVVAVEVTGGPTIDFVPGRKDS 120

Query: 121 NICPKEGRLPDAKKGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTEDP 180
           NICP+EGRLPDAK+GAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWT +P
Sbjct: 121 NICPREGRLPDAKRGAPHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTNEP 180

Query: 181 LKFDNSYFVELLKGEAEGLLKLPTDKALLEDPEFRQYVELYAKDEDAFFKDYAASHKKLS 240
           LKFDNSYF+ELLKGE+EGLLKLPTDKALL+DPEFR+YVELYAKDEDAFF+DYA SHKKLS
Sbjct: 181 LKFDNSYFLELLKGESEGLLKLPTDKALLDDPEFRKYVELYAKDEDAFFRDYAESHKKLS 240

Query: 241 ELGFTPGSA--KAIVKDSIVLAQGAVGVAVAAAVVILSYLYEVRKKSK 287
           ELGFTP SA  K +VKDS VLAQGAVGVAVAAAVVILSY YEVRK+ K
Sbjct: 241 ELGFTPTSARSKVMVKDSTVLAQGAVGVAVAAAVVILSYFYEVRKRMK 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APX3_ARATH1.1e-13481.53L-ascorbate peroxidase 3, peroxisomal OS=Arabidopsis thaliana GN=APX3 PE=1 SV=1[more]
APX4_ORYSJ1.6e-13078.35Probable L-ascorbate peroxidase 4 OS=Oryza sativa subsp. japonica GN=APX4 PE=2 S... [more]
APX3_ORYSJ1.2e-12074.56Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. japonica GN=APX3 PE=3 S... [more]
APX3_ORYSI2.6e-12074.22Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica GN=APX3 PE=2 SV=... [more]
APX5_ARATH7.0e-10567.62L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana GN=APX5 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
Q948P1_9ROSI1.7e-15093.01Peroxisomal ascorbate peroxidase OS=Cucurbita cv. Kurokawa Amakuri GN=pAPX PE=2 ... [more]
Q39780_GOSHI2.0e-14689.93Ascorbate peroxidase OS=Gossypium hirsutum GN=gAPX PE=2 SV=1[more]
A0A0B0MPD9_GOSAR4.4e-14689.58L-ascorbate peroxidase 3, peroxisomal-like protein OS=Gossypium arboreum GN=F383... [more]
A0A0D2SN99_GOSRA7.4e-14689.58Uncharacterized protein OS=Gossypium raimondii GN=B456_005G254100 PE=3 SV=1[more]
A0A0K2SSH7_CITUN1.2e-14388.15Ascorbate peroxidase (Fragment) OS=Citrus unshiu GN=CitAPX3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35000.16.2e-13681.53 ascorbate peroxidase 3[more]
AT4G35970.13.9e-10667.62 ascorbate peroxidase 5[more]
AT3G09640.13.1e-8764.32 ascorbate peroxidase 2[more]
AT1G07890.33.2e-8462.40 ascorbate peroxidase 1[more]
AT4G08390.11.7e-6450.00 stromal ascorbate peroxidase[more]
Match NameE-valueIdentityDescription
gi|778722949|ref|XP_004144841.2|6.9e-16199.65PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis sativus][more]
gi|659094254|ref|XP_008447964.1|1.7e-15998.60PREDICTED: L-ascorbate peroxidase 3, peroxisomal [Cucumis melo][more]
gi|15430584|dbj|BAB64351.1|2.4e-15093.01peroxisomal ascorbate peroxidase [Cucurbita cv. Kurokawa Amakuri][more]
gi|1019946|gb|AAB52954.1|2.8e-14689.93ascorbate peroxidase [Gossypium hirsutum][more]
gi|728813799|gb|KHG01334.1|6.2e-14689.58L-ascorbate peroxidase 3, peroxisomal -like protein [Gossypium arboreum][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002016Haem_peroxidase_pln/fun/bac
IPR002207Peroxidase_I
IPR010255Haem_peroxidase
IPR019793Peroxidases_heam-ligand_BS
IPR019794Peroxidases_AS
Vocabulary: Molecular Function
TermDefinition
GO:0004601peroxidase activity
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0006979response to oxidative stress
GO:0055114oxidation-reduction process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019852 L-ascorbic acid metabolic process
biological_process GO:0009735 response to cytokinin
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005777 peroxisome
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0004601 peroxidase activity
molecular_function GO:0016688 L-ascorbate peroxidase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.370590Cucsa.370590gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.370590.5Cucsa.370590.5-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.370590.5.five_prime_UTR.1Cucsa.370590.5.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.370590.5.CDS.1Cucsa.370590.5.CDS.1CDS
Cucsa.370590.5.CDS.2Cucsa.370590.5.CDS.2CDS
Cucsa.370590.5.CDS.3Cucsa.370590.5.CDS.3CDS
Cucsa.370590.5.CDS.4Cucsa.370590.5.CDS.4CDS
Cucsa.370590.5.CDS.5Cucsa.370590.5.CDS.5CDS
Cucsa.370590.5.CDS.6Cucsa.370590.5.CDS.6CDS
Cucsa.370590.5.CDS.7Cucsa.370590.5.CDS.7CDS
Cucsa.370590.5.CDS.8Cucsa.370590.5.CDS.8CDS
Cucsa.370590.5.CDS.9Cucsa.370590.5.CDS.9CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.370590.5.three_prime_UTR.1Cucsa.370590.5.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002016Haem peroxidase, plant/fungal/bacterialPRINTSPR00458PEROXIDASEcoord: 31..45
score: 2.8E-28coord: 176..191
score: 2.8E-28coord: 152..167
score: 2.8E-28coord: 107..119
score: 2.8E-28coord: 89..106
score: 2.8
IPR002016Haem peroxidase, plant/fungal/bacterialPFAMPF00141peroxidasecoord: 18..224
score: 2.4
IPR002016Haem peroxidase, plant/fungal/bacterialPROFILEPS50873PEROXIDASE_4coord: 31..260
score: 18
IPR002207Plant ascorbate peroxidasePRINTSPR00459ASPEROXIDASEcoord: 49..59
score: 3.2E-105coord: 10..30
score: 3.2E-105coord: 31..46
score: 3.2E-105coord: 128..145
score: 3.2E-105coord: 146..167
score: 3.2E-105coord: 63..87
score: 3.2E-105coord: 88..106
score: 3.2E-105coord: 107..119
score: 3.2E-105coord: 220..244
score: 3.2E-105coord: 168..192
score: 3.2E
IPR010255Haem peroxidaseunknownSSF48113Heme-dependent peroxidasescoord: 11..248
score: 1.39
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 152..162
scor
IPR019794Peroxidase, active sitePROSITEPS00436PEROXIDASE_2coord: 31..42
scor
NoneNo IPR availableGENE3DG3DSA:1.10.420.10coord: 142..234
score: 1.9
NoneNo IPR availableGENE3DG3DSA:1.10.520.10coord: 9..141
score: 3.4
NoneNo IPR availablePANTHERPTHR31356FAMILY NOT NAMEDcoord: 1..284
score: 2.3E
NoneNo IPR availablePANTHERPTHR31356:SF9L-ASCORBATE PEROXIDASE 3, PEROXISOMALcoord: 1..284
score: 2.3E