Cucsa.349130.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.349130.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationscaffold03487 : 446206 .. 453002 (-)
Sequence length914
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTTCATGGAGATGATGATTATAGATGCGTGTTTCGTGCTGGTTTTCTTCTCGAAAGATAAAATTTACAAGTCGCTAATTACTTTCAAATCAGATATAAAACGAGACATTTTGTTGCTTGAGAATCAGCTGCCTTTCCAACTTCTTCAACTGATGTATGAAATATTCCCACTTAAGGTACATATTCTTAATCTATGATCTTAATTAATATATTATATATCCTACCACCTTTGTTTGAACCTTAATAATTTAAATGTTCAACAACATTATTTTGTTATACTGAAAAATTCTATCATATTTTTCATTAATTCTTTAAGTTCAACCTTTTTAAAATTTATTTGCACCAAAATCTATATATATATATGTTTGAGGATTGACTAGTCATAGTTGAAATTGAAAATTTAGCTAGAGATGTATTAGAATTTTTTCAAGTTTGAAGAACTATTAGACGACATCATTTCTTTAAGTCTAGAAACTTGTTAGACGTATGAAATTGGAAGTTTGAAGATCTATTAAACATTTGAAGAGAAAAACAAATGAATTTTATTAGGTCTGAAGAGCACTGAATGATGATCAATCATATTCTAAGTAATGCAAAAAGGAAAAGGCTTGACGTTGGATGATAATCATTCACCAATTATCAGGATGTCTTTTGCTACACATTTTTTTATTGGATATGGTTCACGTACTTTATGAATTCTCTAATAATATACCTTATTAAATAAACTCGAGGTTAATCGATTAACGACAAGAGGAATAATTAATTCACCTTTCTTAATTATATAAATATATACGAGATGAGATCCTCAACATTGCTTTCATCAAAATTTTAATAATTATAATTTGGATGATTTTGGTTCTAACAAGTTGGAAGAGAAACTTTGACTTCACTAATTATAAGCAACTTATCATTTTACTCTAAAGATGAGCCAACGATAACAAACAAGAAGCACATTCTGTATAGAGCCTCATTGTTGTATAAGGATATTAAAAATAAGCAAAATGAAGGAAATGCTTGCGTCCACCCGCCCATTTGTGGTGGTAACCTTTTAAAAAAAAGAAGAATATATAAAGAAACGAACAACGACAGAAATGACGACGACGAATTCAAGCATGTAGTAAATACTCAAATCATTCCACAAGCAACATTACTTCGCGATGCTGGAATAAAAATTCGAAAAAGCAAATAAAAAAGCCTCGAAGACGTGAGTTTTGAAAATGGCGTGTTGAAACTGGCAAGTCTAATCATGAAGCTGAAACAAATTTACTAAATGTGATGGTATTTGAGAAATTGCACGATGGTGTTGGAAATGAAGTGACATCTTTTATAATGATGATGAATAATTTGATCGACATAGATAAAGATGTCGAGCTATTGTCAAAGAGAAATATATGCCAATGCACTTGGAAACAATGAAGACGTGGAAAATTTGTTTAGTGTGCTTGGGAAAGGAGTGCCTATTATATTGATTCGAATAGCAAAATAATAGAAGTACATCATTAAGTGAATGAATATATTTATTGTGATGAATCATGGAATAGATGGTGTGTGCTAATTTTAAACACAAATACTTCGATAACCCATGGTCTATTATCTCATTCAATGCCGCTATTTTCGGTTTCGTTATCCTTATTGTTCAAACAATCTACCAAGTTAATTGTCGACTTCTAAACAAAGAAAATAAAAGGGAATCAATAAACATTATCGTCTTTGAATAATAAGTTGTCATGATATGATAAACACTTCCATTATTATTTTCTTTTTGTACAATGATATGAATATTAACTGTATAATAAATTTAATTTAGACATGCATGCATCCATGTATGTCTTTATCAAGCTTTGTTTTGGATTTTTTTTCGTTTGTATGATTGTCCTACTTTATATTTATATATATGTATGTATTGAGGATTTGAAAGGATGTATATAGCTTAATCACATTTTCAAGCATTGTATTTAATAAATTGTTCAATCTTAACTTTGACGTGTCAAATTATTTTTTTTTTCGGAAGAATATGGTTAATGTGGAGAATGATACATATTAAGTATTACTTTTTTAAAAAAGAGATTAATTGAAAATGTAGCAACCAAATCAAAATGATTAGCAAAATATTGTATAATTCAAAACACTAATTTAGCAAAATTACAGACACCATATTTTTTCTTAGCGGCGAAGAAGAGAAAAAAGAAAAGGAAACTCCATTTTTTCTCTTAACTTTTGAAGAAAAATCATTCTCACTCTGTCTTGACTCTCCCCATCTTCCCGACCTTCAATCTCTCTCGAATCTCTCCGGCCGACATTTCCCAACCTACCCACTGCCCCAATTTTCCATTACCTGCTTTTTATCTTTCGAATTTCAGCTGGGAAAAACAAAAATACCCAACCCATCAAACTCCGTAAGAGAAAACACCACGAGGCGTTCGCTAGTTGTTGGGCTAAAAAAGAGGGCGATCTCCTTATCAACCATAGGCACCAGGAAGATTCCGCCGCCGCTGCTGCACCTGTCGTTCACGATGCCGATGGCTTTGATGGCGGCTTTTCTTCCCTCCATAGCATTTTACAGTGGGCTATACGTACTCTTTTATTCATGATTGTATTTCATGCTCAGATATAAGGTCATGCCTTTAAATGGTAGTATTAGCAATAATCACTGGAATTCATGCCAATGGGCCTTGTGTAAGACATTGGGGGAATTACTTGAGATGTCTTTGCAACATTGTTCGGCACAAGAATACTTCAAACTTAACTTAATTTATGATTCTAGACTTTAAAGATTCTTTCTTCATATTATAATGTGGCATAAGTTATACATTTTGTCTATCAAAGAAGAGTTCTTACCGTGAATAGAAGATGAAGGTAGGGTTGAGGTAAGTTAGTTGTAACCTAACCTAACCTAACTCAGCAACGGTAGACAGGAAGAAGATGGTGAAATTCTCTTGCAACTATCCATGTGTGAGGTTGATTATTTAAACTAAATTAACTATTTTTATTGAGAAAAATTAAACGAAAGAACAGAAAGACATGCAGAAAACCCAAAGAGATAAAAACATTACTAAAGGGTCAATCAAGAAAAATAATGCCTATAGAATAATTTAAAAAACAAAAATTACTAGGCTTCCTATCCAACTCATTATATTAAACTTTGTATTCTACCTTTCCCCAAAAGATTCCACGTGATAGCGCCCTCATCCATGTGTCAACGACGAGGAACAATATATCCTTTCCACCGTGGGGTTGATGAAGAAGAAACTCACTGATCATATCTTTGATGCCTAATTGTATCTGCTTGATGACGACTAAACCCAAAGGAAAAGGAAAACAAACTTCCTCAAATGTCTATAATATGCTTTCTATACAGAAAAAAAAATTAATTTGTCAAAATAATTTCAATAATTTTTTAAACCTTATCTAACACAAAATTCCTAAAAGCTATTACAAAATTATGGAGGTTTAACATTACGTAGTCACATGCACACTAATTAAGAAGTCGTGTAGAATGTTTCTTTCCGACAAAAATTCATTTTCACTTGTGGTGCTTTCTTCTTATATCGAACTCACAAGTGAGGGATCGAGTTAAGTAATATATTTAATATTAAATTTATCTTCATTATTTATTAGCTTAATCTATTGGATCAATCAACAACGATTGAATTGATTTTTTTAAAATTATCTCATCTTAGGTTGTTATAATATATTAAGTGAATATGACTTTGGCATCAAAAGCACAACTCACATTTCGTTTCAGACAACTTTGAGTCCTAAGAAATTTACCATTTTTGTTGTTCAAAGTGAAGTTTAATTCATTTTTTTAAAAAATAATCCATATTAAAACTTAGATTTTTTATAAAAGAAATAAACTAATCAGAGTTGGGATGAAATTATGGTAAATAACAAAATTGTTTAAAATAATTATATGAAAATTTGATATATTTCATATATATTTTGCTTTATATTAAAAATGTGTTATTAATTTAATAAAACTCAATAATTAATAAAAGAAAACATTTGAAATCAAGTGAGAACATTAAAAGATGAAGAAAGCAAAGTAAAAGCAAAAGAGATGAAAGTGAATTCTTGCCAATCAAGGCAACTTTTATTCATCCTAAGATTGATGCCCAATGTTTACAAATTAGATTTATTCAAAAAAAGAAAAATGTATGTTTCATTCCATTTTATATATTCATTCAATTTACTGATCACATTTCAAAAAGACAGAAACCTTTAATAAATATTATTATATCAATTATTCATTTTTTTCAAACAAAATATATAATGAAAAAACTTCTCAAAAATATTCATGAGTACAGCTAAATATACATATCCTGAAATAATAATATAAACTACCTTATATGTCTATTGGTATAATAATGGATACATATATAATATAGTTTGATCTATCCAGATAAATATATTTTATAAATATTTTGATATTTTTGCTATATTTAAAAATAATATTAGTAGTAACACAATATAATATATATTATAAACTTATTTGATATTGTAAAAAATTAATTTTGGTAAGTAAACTCTAACTCATTTTTATAATTTTTAATTACGTTTTATAAAATAAAAACATTTCGAATCATTTTTTTAAAGTAAGATCTAACTTTTGAATCCAGTTTCAAATACACATCCAAGAAAGGAATTTGAGTTTCTTTTTTCAAATATTATTGTCTACCAAACAAAATTCTTAACTTTCTAAAGAATTTAGTTGTGATCTTAATACTAACATTAGAGTTTATTTGAATTTGATTGATAGATCAATGTCCATACAAAAATTGATGGACTGAAGTATTTTGTTATATTTACAAAATAAGTTAAACAATACCCCTTAAAAATAGAAAAGGTATTTTCAATTTCACTTTATACAAAGTGGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

AGTTCATGGAGATGATGATTATAGATGCGTGTTTCGTGCTGGTTTTCTTCTCGAAAGATAAAATTTACAAGTCGCTAATTACTTTCAAATCAGATATAAAACGAGACATTTTGTTGCTTGAGAATCAGCTGCCTTTCCAACTTCTTCAACTGATGTATGAAATATTCCCACTTAAGGATCAAAATACAACTTTGACTTCACTTATAAGCAACTTATTGTTTGTCGAGAAAGATGAGCTAGCGATAGTGGAAAAGAAGCACATTTTACATATGTATAGAGCGTCATTATTGTATCCATCTACTCTAAGCTATCCCAATATGGATGAAATAAAGAAAAATAACAAGGACGACAAATTCGGGCTAAAGTGTCAACTCATTCCACAGGCAACACTACTTCGCGAAGCTGGAATAAGATTTCGAAAAAGTGAAAACAAAAGCCTTGAAAACGTGAGTTTTGAAAAAGGCGTGTTGAAACTGCCACTTCTAATTGTAGATGATAATACCGAAACAAATTTACTAAATGTGATGGCATTTGAGAAACTCCATGACGTTGGAAGTCAAGTGACATCTTTTGTAGTGTTGATGAATAATTTGATCGACATTGATAAAGATGTCGAGCTATTGTCGAATGATAATATAATAGCCAATGCACTTGGGAACAATGAAGAAGCGGCGAATTTGTTTAGTGTACTTGGGAAAGGAGTGTCTCTGGATTTGGGGAGCAATAATTTAACAGAAGTGCATCAATTGGTGAATATACATTGTGATGATTCATGGAATAGATGGTGGGCTAATCTTAAACACACTTACTTTCAAAACCCATGGGCTATTATCTCATTCTTTGGTGCTATTTTTGGTTTCGCTATCCTTATTGTTCAAGCCGTCTACCAAATTGTTGACTTCCACACAAAATAG

Coding sequence (CDS)

ATGGAGATGATGATTATAGATGCGTGTTTCGTGCTGGTTTTCTTCTCGAAAGATAAAATTTACAAGTCGCTAATTACTTTCAAATCAGATATAAAACGAGACATTTTGTTGCTTGAGAATCAGCTGCCTTTCCAACTTCTTCAACTGATGTATGAAATATTCCCACTTAAGGATCAAAATACAACTTTGACTTCACTTATAAGCAACTTATTGTTTGTCGAGAAAGATGAGCTAGCGATAGTGGAAAAGAAGCACATTTTACATATGTATAGAGCGTCATTATTGTATCCATCTACTCTAAGCTATCCCAATATGGATGAAATAAAGAAAAATAACAAGGACGACAAATTCGGGCTAAAGTGTCAACTCATTCCACAGGCAACACTACTTCGCGAAGCTGGAATAAGATTTCGAAAAAGTGAAAACAAAAGCCTTGAAAACGTGAGTTTTGAAAAAGGCGTGTTGAAACTGCCACTTCTAATTGTAGATGATAATACCGAAACAAATTTACTAAATGTGATGGCATTTGAGAAACTCCATGACGTTGGAAGTCAAGTGACATCTTTTGTAGTGTTGATGAATAATTTGATCGACATTGATAAAGATGTCGAGCTATTGTCGAATGATAATATAATAGCCAATGCACTTGGGAACAATGAAGAAGCGGCGAATTTGTTTAGTGTACTTGGGAAAGGAGTGTCTCTGGATTTGGGGAGCAATAATTTAACAGAAGTGCATCAATTGGTGAATATACATTGTGATGATTCATGGAATAGATGGTGGGCTAATCTTAAACACACTTACTTTCAAAACCCATGGGCTATTATCTCATTCTTTGGTGCTATTTTTGGTTTCGCTATCCTTATTGTTCAAGCCGTCTACCAAATTGTTGACTTCCACACAAAATAG

Protein sequence

MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAILIVQAVYQIVDFHTK*
BLAST of Cucsa.349130.1 vs. Swiss-Prot
Match: Y3720_ARATH (UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.0e-31
Identity = 101/327 (30.89%), Postives = 167/327 (51.07%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFF---------SKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMY 60
           M MM++D CF+L+ F         S+D I+ S+    S I+ D+LLLENQ+PF +LQ +Y
Sbjct: 134 MFMMVLDGCFILMVFLIMSGNIELSEDPIF-SIPWLLSSIQSDLLLLENQVPFFVLQTLY 193

Query: 61  ---EIFPLKDQNTTLTSLISNLLFVEK---DELAIVEKKHILHMYRASLLYPST-----L 120
              +I    D N        N +  E    ++    + KH+L + R + L P+T      
Sbjct: 194 VGSKIGVSSDLNRIAFHFFKNPIDKEGSYWEKHRNYKAKHLLDLIRETFL-PNTSESDKA 253

Query: 121 SYPNMD----EIKKNNKDDKFGLKCQLIPQATLLREAGIRFR--KSENKSLENVSFEKGV 180
           S P++     E K  N          LI  A  LR  GI+FR  +S+  S+ NV  +K  
Sbjct: 254 SSPHVQVQLHEGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNK 313

Query: 181 LKLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDN-II 240
           L++P L  D    +  LN +AFE+ + D  +++T+++V M  L++ ++DV  L ND  II
Sbjct: 314 LQIPQLRFDGFISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLII 373

Query: 241 ANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNP 300
            N  G+N E +  F  + K V  ++ ++ L  V + VN +    +N  WA  +HT+F++P
Sbjct: 374 ENHFGSNNEVSEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESP 433

BLAST of Cucsa.349130.1 vs. Swiss-Prot
Match: Y3264_ARATH (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.6e-14
Identity = 50/171 (29.24%), Postives = 91/171 (53.22%), Query Frame = 1

Query: 124 IPQATLLREAGIRFRKSENKSLENVSFEK--GVLKLPLLIVDDNTETNLLNVMAFEKLHD 183
           IP  + L +AG+RF+ + + ++  V+F+   G   LP++ +D NTET L N++A+E  + 
Sbjct: 345 IPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNT 404

Query: 184 VGSQV-TSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSN 243
            G  V T +  L+N +ID ++DV LL    ++ + L +++EAA +++ + K V L     
Sbjct: 405 SGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRL-TKVG 464

Query: 244 NLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAILIVQ 292
            L +  + VN +    W      L   Y    W I++F  A+    ++ +Q
Sbjct: 465 FLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFLAAVLLLMLVSLQ 514

BLAST of Cucsa.349130.1 vs. TrEMBL
Match: A0A0A0K9F5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G089270 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 4.8e-135
Identity = 245/254 (96.46%), Postives = 248/254 (97.64%), Query Frame = 1

Query: 49  LMYEIFPLKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI 108
           ++  IF  KDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI
Sbjct: 156 MILHIFYSKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI 215

Query: 109 KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTET 168
           KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVL LP LIVDDNT+T
Sbjct: 216 KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLTLPSLIVDDNTKT 275

Query: 169 NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV 228
           NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV
Sbjct: 276 NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV 335

Query: 229 LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL 288
           LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL
Sbjct: 336 LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL 395

Query: 289 IVQAVYQIVDFHTK 303
           IVQAVYQIVDFHTK
Sbjct: 396 IVQAVYQIVDFHTK 409

BLAST of Cucsa.349130.1 vs. TrEMBL
Match: E5GB49_CUCME (Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 5.0e-108
Identity = 213/311 (68.49%), Postives = 248/311 (79.74%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKDQN 60
           MEMMIIDACF+LVFFSKDK YKSL+T +SDIKRDILLLENQLPFQLLQL+Y+I P+KDQN
Sbjct: 155 MEMMIIDACFILVFFSKDKSYKSLMTLRSDIKRDILLLENQLPFQLLQLLYKILPIKDQN 214

Query: 61  TTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKK-------NNK 120
            +LTSLI  L FV+KDEL +   KHIL MYR  LL P  +     DE +K       N K
Sbjct: 215 KSLTSLICKLWFVKKDELTVKGGKHILEMYRMLLLDPIPIVLSERDESQKKAEGTKGNKK 274

Query: 121 D-DKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLN 180
           + D+  L  Q+IPQATLL +AGI+FR+SE +SL ++ F+ GVL+LP L VDD+TET LLN
Sbjct: 275 EKDESVLNSQIIPQATLLHDAGIKFRRSETESLIDIGFKNGVLELPHLTVDDDTETKLLN 334

Query: 181 VMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSVLGK 240
           VMAFEKLH +V S VTSFVVLMNNLIDIDKDVELLS + II NALGN+E+AA LF+VLGK
Sbjct: 335 VMAFEKLHGNVRSYVTSFVVLMNNLIDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGK 394

Query: 241 GVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAILIVQ 300
           GV+LDL S N+ +VH+LVN HCD   NRW ANLKH YFQNPWAIIS  GAIFGF ILIVQ
Sbjct: 395 GVALDLES-NIAKVHRLVNKHCDGRCNRWCANLKHNYFQNPWAIISLIGAIFGFLILIVQ 454

Query: 301 AVYQIVDFHTK 303
           AVYQI+D+HT+
Sbjct: 455 AVYQIIDYHTR 464

BLAST of Cucsa.349130.1 vs. TrEMBL
Match: A0A0A0KAA3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G091270 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.1e-102
Identity = 197/249 (79.12%), Postives = 221/249 (88.76%), Query Frame = 1

Query: 58  DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKKNNKDDKF 117
           DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYP TL+YP  DEIK+NNKDD+F
Sbjct: 442 DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPPTLNYPIRDEIKENNKDDEF 501

Query: 118 GLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFE 177
           GLKCQ+IPQATLLRE GIRF+KS+NKSLENVSFEKGVL LP LIVDDNT+TNLLNVMAFE
Sbjct: 502 GLKCQIIPQATLLREVGIRFQKSKNKSLENVSFEKGVLTLPSLIVDDNTKTNLLNVMAFE 561

Query: 178 KLHD-VGSQVTSFVVLMNNLIDIDKDVELLSND--NIIANALGNNEEAANLFSVLGKGVS 237
           KLHD VGSQVTSFVVLM+NLI IDKDV+LLSN   NIIANA  N+ +A NLFS LGKGV 
Sbjct: 562 KLHDEVGSQVTSFVVLMHNLIRIDKDVQLLSNGNINIIANAFMNDRDAVNLFSALGKGVY 621

Query: 238 LDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGA-IFGFAILIVQAV 297
           ++  +NNLT+++  VN +CD SWN+W A+LK +YFQNPW+I+S F A +FGFAILIVQAV
Sbjct: 622 ME--TNNLTKIYISVNQYCDKSWNKWCADLKQSYFQNPWSILSLFHAGVFGFAILIVQAV 681

Query: 298 YQIVDFHTK 303
           YQIVDFHTK
Sbjct: 682 YQIVDFHTK 688

BLAST of Cucsa.349130.1 vs. TrEMBL
Match: A0A0A0KAA3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G091270 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-33
Identity = 118/312 (37.82%), Postives = 169/312 (54.17%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKD-- 60
           +++MI+D CFVL   S+            DIKRD++LLENQLP QLL+ ++ +    +  
Sbjct: 130 LKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTNENE 189

Query: 61  -QNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKKNN----- 120
            +N  L ++I + + +  + L++    HIL +YR+SLL P+       DE  +N      
Sbjct: 190 KENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCK---QDETHENTITILE 249

Query: 121 ---KDDKFGLKCQL-IPQATLLREAGIRFRKSENKSLENVSFE--KGVLKLPLLIVDDN- 180
               +++    CQ  I  AT LR+AGI+F KS   +L +VSF+  + VL+LP L +DD  
Sbjct: 250 WTLTNEENVEYCQSNIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDIL 309

Query: 181 TETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNII-ANALGNNEEAA 240
           TE  L+N+MAFEKLH  V  QVTSFVVLM NLI ++KDV LL++  I+ +N + +     
Sbjct: 310 TEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNVIHDGNGVV 369

Query: 241 NLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQ--NPWAIISFFGA 294
            LF+VL KG +  L S+ +                 W  NLK    Q  N W IIS    
Sbjct: 370 QLFNVLAKGQTKYLESHII-----------------WLENLKLKVPQTKNSWQIISIALF 421


HSP 2 Score: 212.2 bits (539), Expect = 8.6e-52
Identity = 126/324 (38.89%), Postives = 189/324 (58.33%), Query Frame = 1

Query: 1   MEMMIIDACFVLV-----------FFSKDKIYKS--LITFKSDIKRDILLLENQLPFQLL 60
           +++MI+D CF+L            +   D I+ +   +     IKRD+L+LENQLP Q+L
Sbjct: 118 LQLMILDGCFMLEILRSATHTLEDYAQNDPIFSNHGKLHIMPFIKRDMLMLENQLPMQVL 177

Query: 61  QLMYEIFPLKDQNTTLTSLISNLLFVE-----KDELAIVEKKHILHMYRASLLY-----P 120
           + +  +    D N T      N L ++          +    H+L +YR SLL      P
Sbjct: 178 EKLVAV----DSNRTKDEEFVNKLILKFCSPNTPVSCLGSCLHVLDVYRKSLLQDVPGRP 237

Query: 121 STLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKL 180
               +       + + DD       +I  A  L EAGIRF+K +  SL++++F  G+LKL
Sbjct: 238 RRKRHRKPRSTHREDGDD-------IIRSAMELNEAGIRFKKCKTVSLKDITFHGGILKL 297

Query: 181 PLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANAL 240
           P++IVDD TE+  LN++AFE+LH   G++VTS++  M+N+ID  KDV LL +  II NAL
Sbjct: 298 PVIIVDDATESMFLNLIAFERLHVGAGNEVTSYIFFMDNIIDSQKDVALLHSTGIIQNAL 357

Query: 241 GNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAII 300
           G+++  ANLF+ L K ++LD  S+ L EVH+ VN +C  +WN W ANL HTYF+NPWAI+
Sbjct: 358 GSDKAVANLFNSLSKDITLDPDSS-LDEVHKKVNRYCKKAWNEWRANLIHTYFRNPWAIL 417

BLAST of Cucsa.349130.1 vs. TrEMBL
Match: A0A0D2SPR7_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_006G003900 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 7.3e-51
Identity = 130/326 (39.88%), Postives = 193/326 (59.20%), Query Frame = 1

Query: 1   MEMMIIDACFVL------------------VFFSKDKIYKSLITFKSDIKRDILLLENQL 60
           M++MI+D CF+L                  +F +  KI+  +I F   IKRD+L+LENQL
Sbjct: 152 MQLMILDGCFMLEILCFDTHTMEDYAETDPIFSNHGKIH--IIPF---IKRDMLMLENQL 211

Query: 61  PFQLLQLMYEIFPLKDQNTTLTSLISNLLFVE--KDELAIV---EKKHILHMYRASLL-- 120
           P QLL  +  +    D N T      N L ++      A+       H+L +YR SLL  
Sbjct: 212 PMQLLHSLVAV----DSNGTKDEEFMNKLILKFYSSNTAVSCVGSCLHVLEVYRKSLLPD 271

Query: 121 YPSTLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVL 180
            PS          ++ + +D       +I  A  L EAGIRF+KS+  SL++++F  G+L
Sbjct: 272 IPSCRQQRKRHWHRRPHYEDGD----DIIRSAMELNEAGIRFKKSKTISLKDITFRGGIL 331

Query: 181 KLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIAN 240
           KLP++I+DD TE+  LN++AFE+LH  +G++++S+++ M+N+ID +KDV LL +  II N
Sbjct: 332 KLPVIIIDDATESMFLNLVAFERLHVGLGNEISSYILFMDNIIDNEKDVALLHSKGIIQN 391

Query: 241 ALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWA 300
           AL +++  AN+F+ L K ++LD  +NNL EV ++VN +C   WN W ANL HTYF NPWA
Sbjct: 392 ALESDKAVANMFNSLSKDITLD-PNNNLYEVRKMVNGYCKKPWNEWRANLIHTYFTNPWA 451

BLAST of Cucsa.349130.1 vs. TAIR10
Match: AT3G50120.1 (AT3G50120.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 159.8 bits (403), Expect = 2.6e-39
Identity = 110/341 (32.26%), Postives = 177/341 (51.91%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFS-------------KDKIYKSLITFKSDIKRDILLLENQLPFQLL 60
           +EM+++D CFVL  F               D ++    +  S I+RD+++LENQLP  +L
Sbjct: 189 IEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAMRGSMHS-IQRDMVMLENQLPLFVL 248

Query: 61  QLMYEIF-------------------PLKDQNTTLT----SLISNLLFVEK--DELAIVE 120
             + E+                    PL   +  LT    S + N L  +K  D  A + 
Sbjct: 249 NRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSFDPFADMG 308

Query: 121 KKHILHMYRASLLYPSTLSYPNMDEIKKNNKDDKFGLKC--QLIPQATLLREAGIRFRKS 180
           + H L ++R SLL  S    P +   K+ +++ +   K   QLI   T L+EAGI+FR+ 
Sbjct: 309 ELHCLDVFRRSLLRSSPKPEPRLTR-KRWSRNTRVADKRRQQLIHCVTELKEAGIKFRRR 368

Query: 181 ENKSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDI 240
           +     ++ F+ G L++P L++ D T++  LN++AFE+ H D  + +TS+++ M+NLID 
Sbjct: 369 KTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFEQCHIDSSNDITSYIIFMDNLIDS 428

Query: 241 DKDVELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNR 300
            +DV  L    II + LG++ E A+LF+ L + V  D   + L+ +   VN + D  WN 
Sbjct: 429 HEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNA 488

BLAST of Cucsa.349130.1 vs. TAIR10
Match: AT3G50130.1 (AT3G50130.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 151.8 bits (382), Expect = 7.0e-37
Identity = 109/332 (32.83%), Postives = 176/332 (53.01%), Query Frame = 1

Query: 2   EMMIIDACFVLVFF-SKDKIYKSLITFKSD-----------IKRDILLLENQLPFQLLQL 61
           EM+++D CFVL  F   D+ +  L   ++D           I+RD+++LENQLP  +L  
Sbjct: 226 EMLVLDGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNR 285

Query: 62  MYEIFPLKDQNTTLTSLISNLLF----------------VEKDEL--AIVEKK----HIL 121
           + EI   K   T L S ++   F                +E+D+    I +K     H L
Sbjct: 286 LLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKTDDSLEQDKFFNPIADKDKGELHCL 345

Query: 122 HMYRASLLYPSTLSYPNMDEIK---KNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKS 181
            ++R +LL P +   P +  ++   +    DK   + QLI   T LREAGI+FR  +   
Sbjct: 346 DVFRRNLLRPCSNPEPRLSRMRWSWRTRVADK--RQQQLIHCVTELREAGIKFRTRKTDR 405

Query: 182 LENVSFEKGVLKLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDV 241
             ++ F+ G L++P L++ D T++   N++AFE+ H D  + +TS+++ M+NLID  +DV
Sbjct: 406 FWDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSNDITSYIIFMDNLIDSSEDV 465

Query: 242 ELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWAN 296
             L    II + LGN+ E A+LF+ L + V+ D  ++ L+++   V+ +    WN   A 
Sbjct: 466 RYLHYCGIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYSRKWNVLKAI 525

BLAST of Cucsa.349130.1 vs. TAIR10
Match: AT3G50140.1 (AT3G50140.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 151.0 bits (380), Expect = 1.2e-36
Identity = 103/333 (30.93%), Postives = 175/333 (52.55%), Query Frame = 1

Query: 2   EMMIIDACFVLVFFS-------------KDKIYKSLITFKSDIKRDILLLENQLPFQLLQ 61
           +M+++D CFVL  F               D ++    +  S I+RD+L+LENQLP  +L 
Sbjct: 200 QMLVLDGCFVLDLFRGAYEGFSKLGYDRNDPVFAMRGSMHS-IRRDMLMLENQLPLFVLN 259

Query: 62  LMYEIFPLKDQNTTLTSLISNLLF------------VEKDE---------LAIVEKK--H 121
            + E+       T L + ++   F            +E  +         +A  EK+  H
Sbjct: 260 RLLELQLGTQYQTGLVAQLAVRFFNPLMPTYMSSTKIENSQENNNKFFNPIADKEKEELH 319

Query: 122 ILHMYRASLLYPSTLSYPNMDEIKKNNKD---DKFGLKCQLIPQATLLREAGIRFRKSEN 181
            L ++R SLL PS    P +   + + K    DK   + QL+   T LREAGI+F++ ++
Sbjct: 320 CLDVFRRSLLQPSLKPDPRLSRSRWSRKPLVADK--RQQQLLHCVTELREAGIKFKRRKS 379

Query: 182 KSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDK 241
               ++ F+ G L++P L++ D T++   N++A+E+ H D  + +TS+++ M+NLID  +
Sbjct: 380 DRFWDIQFKNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDSTNDITSYIIFMDNLIDSAE 439

Query: 242 DVELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWW 295
           D+  L   +II + LGN+ E A++F+ L + V+ DL +  L+E+   V+ + +  WN   
Sbjct: 440 DIRYLHYYDIIEHWLGNDSEVADVFNRLCQEVAFDLENTYLSELSNKVDRYYNRKWNVLK 499

BLAST of Cucsa.349130.1 vs. TAIR10
Match: AT3G50160.1 (AT3G50160.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 145.6 bits (366), Expect = 5.0e-35
Identity = 96/313 (30.67%), Postives = 156/313 (49.84%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFS-------------KDKIYKSLITFKSDIKRDILLLENQLPFQLL 60
           +EM+++D  F++  F               D ++  +      I+RD+++LENQLP+ +L
Sbjct: 190 IEMLVLDGVFIIEIFKGTSEGFQEIGYAPNDPVF-GMRGLMQSIRRDMVMLENQLPWSVL 249

Query: 61  QLMYEIFP---LKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPN 120
           + + ++     L   N  L       L   ++ L      H L + R  LL  S  S  +
Sbjct: 250 KGLLQLQRPDVLDKVNVQLFQPFFQPLLPTREVLTEEGGLHCLDVLRRGLLQSSGTSDED 309

Query: 121 MDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDD 180
           M  + K  +        QLI   T LR AG+ F + E     ++ F+ G LK+P L++ D
Sbjct: 310 MSMVNKQPQ--------QLIHCVTELRNAGVEFMRKETGHFWDIEFKNGYLKIPKLLIHD 369

Query: 181 NTETNLLNVMAFEKLHDVGS-QVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAA 240
            T++  LN++AFE+ H   S ++TS+++ M+NLI+  +DV  L +  II N LG++ E +
Sbjct: 370 GTKSLFLNLIAFEQCHIKSSKKITSYIIFMDNLINSSEDVSYLHHYGIIENWLGSDSEVS 429

Query: 241 NLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIF 297
           +LF+ LGK V  D     L+ +   VNI+    WN   A L+H YF NPWA  SF  A+ 
Sbjct: 430 DLFNGLGKEVIFDPNDGYLSALTGEVNIYYRRKWNYLKATLRHKYFNNPWAYFSFIAAVT 489

BLAST of Cucsa.349130.1 vs. TAIR10
Match: AT3G50170.1 (AT3G50170.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 142.1 bits (357), Expect = 5.5e-34
Identity = 102/339 (30.09%), Postives = 166/339 (48.97%), Query Frame = 1

Query: 2   EMMIIDACFVLVFFS-------------KDKIYKSLITFKSDIKRDILLLENQLPFQLLQ 61
           EM+++D CFVL  F               D ++ ++      I+RD+++LENQLP  +L 
Sbjct: 200 EMLVLDGCFVLELFRGTVEGFTEIGYARNDPVF-AMRGLMHSIQRDMIMLENQLPLFVLD 259

Query: 62  LMYEIF-------------------PLKDQNTTLT----SLISNLLFVEKDELAIVEKKH 121
            + E+                    PL      LT    S + N L    D L    + H
Sbjct: 260 RLLELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGELH 319

Query: 122 ILHMYRASLLYPSTLSYPNMDEIKKN---NKDDKFGLKCQLIPQATLLREAGIRFRKSEN 181
            L ++R SLL  S    PN   + K    N       + QL+   T LREAG++FRK + 
Sbjct: 320 CLDVFRRSLLQSSPT--PNTRSLLKRLTRNTRVVDKRQQQLVHCVTELREAGVKFRKRKT 379

Query: 182 KSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDK 241
               ++ F+ G L++P L++ D T++   N++AFE+ H +  + +TS+++ M+NLI+  +
Sbjct: 380 DRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIESSNHITSYIIFMDNLINSSE 439

Query: 242 DVELLSNDNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWW 301
           DV  L    II + LG++ E A+LF+ L + V  D   ++L+ +   VN + +  WN   
Sbjct: 440 DVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLK 499

BLAST of Cucsa.349130.1 vs. NCBI nr
Match: gi|700191196|gb|KGN46400.1| (hypothetical protein Csa_6G089270 [Cucumis sativus])

HSP 1 Score: 488.8 bits (1257), Expect = 6.8e-135
Identity = 245/254 (96.46%), Postives = 248/254 (97.64%), Query Frame = 1

Query: 49  LMYEIFPLKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI 108
           ++  IF  KDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI
Sbjct: 156 MILHIFYSKDQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEI 215

Query: 109 KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTET 168
           KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVL LP LIVDDNT+T
Sbjct: 216 KKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLTLPSLIVDDNTKT 275

Query: 169 NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV 228
           NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV
Sbjct: 276 NLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSV 335

Query: 229 LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL 288
           LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL
Sbjct: 336 LGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAIL 395

Query: 289 IVQAVYQIVDFHTK 303
           IVQAVYQIVDFHTK
Sbjct: 396 IVQAVYQIVDFHTK 409

BLAST of Cucsa.349130.1 vs. NCBI nr
Match: gi|778711703|ref|XP_011656783.1| (PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus])

HSP 1 Score: 425.6 bits (1093), Expect = 7.1e-116
Identity = 211/214 (98.60%), Postives = 212/214 (99.07%), Query Frame = 1

Query: 89  MYRASLLYPSTLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENV 148
           MYRASLLYPSTLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENV
Sbjct: 1   MYRASLLYPSTLSYPNMDEIKKNNKDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENV 60

Query: 149 SFEKGVLKLPLLIVDDNTETNLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSN 208
           SFEKGVL LP LIVDDNT+TNLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSN
Sbjct: 61  SFEKGVLTLPSLIVDDNTKTNLLNVMAFEKLHDVGSQVTSFVVLMNNLIDIDKDVELLSN 120

Query: 209 DNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTY 268
           DNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTY
Sbjct: 121 DNIIANALGNNEEAANLFSVLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTY 180

Query: 269 FQNPWAIISFFGAIFGFAILIVQAVYQIVDFHTK 303
           FQNPWAIISFFGAIFGFAILIVQAVYQIVDFHTK
Sbjct: 181 FQNPWAIISFFGAIFGFAILIVQAVYQIVDFHTK 214

BLAST of Cucsa.349130.1 vs. NCBI nr
Match: gi|307135819|gb|ADN33691.1| (hypothetical protein [Cucumis melo subsp. melo])

HSP 1 Score: 399.1 bits (1024), Expect = 7.1e-108
Identity = 213/311 (68.49%), Postives = 248/311 (79.74%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKDQN 60
           MEMMIIDACF+LVFFSKDK YKSL+T +SDIKRDILLLENQLPFQLLQL+Y+I P+KDQN
Sbjct: 155 MEMMIIDACFILVFFSKDKSYKSLMTLRSDIKRDILLLENQLPFQLLQLLYKILPIKDQN 214

Query: 61  TTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKK-------NNK 120
            +LTSLI  L FV+KDEL +   KHIL MYR  LL P  +     DE +K       N K
Sbjct: 215 KSLTSLICKLWFVKKDELTVKGGKHILEMYRMLLLDPIPIVLSERDESQKKAEGTKGNKK 274

Query: 121 D-DKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLN 180
           + D+  L  Q+IPQATLL +AGI+FR+SE +SL ++ F+ GVL+LP L VDD+TET LLN
Sbjct: 275 EKDESVLNSQIIPQATLLHDAGIKFRRSETESLIDIGFKNGVLELPHLTVDDDTETKLLN 334

Query: 181 VMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSVLGK 240
           VMAFEKLH +V S VTSFVVLMNNLIDIDKDVELLS + II NALGN+E+AA LF+VLGK
Sbjct: 335 VMAFEKLHGNVRSYVTSFVVLMNNLIDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGK 394

Query: 241 GVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAILIVQ 300
           GV+LDL S N+ +VH+LVN HCD   NRW ANLKH YFQNPWAIIS  GAIFGF ILIVQ
Sbjct: 395 GVALDLES-NIAKVHRLVNKHCDGRCNRWCANLKHNYFQNPWAIISLIGAIFGFLILIVQ 454

Query: 301 AVYQIVDFHTK 303
           AVYQI+D+HT+
Sbjct: 455 AVYQIIDYHTR 464

BLAST of Cucsa.349130.1 vs. NCBI nr
Match: gi|659120434|ref|XP_008460192.1| (PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo])

HSP 1 Score: 399.1 bits (1024), Expect = 7.1e-108
Identity = 213/311 (68.49%), Postives = 248/311 (79.74%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKDQN 60
           MEMMIIDACF+LVFFSKDK YKSL+T +SDIKRDILLLENQLPFQLLQL+Y+I P+KDQN
Sbjct: 616 MEMMIIDACFILVFFSKDKSYKSLMTLRSDIKRDILLLENQLPFQLLQLLYKILPIKDQN 675

Query: 61  TTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKK-------NNK 120
            +LTSLI  L FV+KDEL +   KHIL MYR  LL P  +     DE +K       N K
Sbjct: 676 KSLTSLICKLWFVKKDELTVKGGKHILEMYRMLLLDPIPIVLSERDESQKKAEGTKGNKK 735

Query: 121 D-DKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLN 180
           + D+  L  Q+IPQATLL +AGI+FR+SE +SL ++ F+ GVL+LP L VDD+TET LLN
Sbjct: 736 EKDESVLNSQIIPQATLLHDAGIKFRRSETESLIDIGFKNGVLELPHLTVDDDTETKLLN 795

Query: 181 VMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNIIANALGNNEEAANLFSVLGK 240
           VMAFEKLH +V S VTSFVVLMNNLIDIDKDVELLS + II NALGN+E+AA LF+VLGK
Sbjct: 796 VMAFEKLHGNVRSYVTSFVVLMNNLIDIDKDVELLSENKIIDNALGNDEDAAKLFTVLGK 855

Query: 241 GVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGAIFGFAILIVQ 300
           GV+LDL S N+ +VH+LVN HCD   NRW ANLKH YFQNPWAIIS  GAIFGF ILIVQ
Sbjct: 856 GVALDLES-NIAKVHRLVNKHCDGRCNRWCANLKHNYFQNPWAIISLIGAIFGFLILIVQ 915

Query: 301 AVYQIVDFHTK 303
           AVYQI+D+HT+
Sbjct: 916 AVYQIIDYHTR 925

BLAST of Cucsa.349130.1 vs. NCBI nr
Match: gi|659120434|ref|XP_008460192.1| (PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo])

HSP 1 Score: 184.9 bits (468), Expect = 2.1e-43
Identity = 123/308 (39.94%), Postives = 183/308 (59.42%), Query Frame = 1

Query: 1   MEMMIIDACFVLVFFSKDKIYKSLITFKSDIKRDILLLENQLPFQLLQLMYEIFPLKDQN 60
           +++MI+D CFVL   S+            DIKRD++LLENQLP QLL+ ++ +     +N
Sbjct: 128 LKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHALTTTTTEN 187

Query: 61  TT----LTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTL----SYPNMDEIKKNN 120
                 L ++I + + +  + L++    HIL MYR+SLL P+      ++ NM  I +  
Sbjct: 188 ENEHLDLEAMIWSWMRLSGEILSVRNPLHILDMYRSSLLSPTICKQDETHENMIAILEWT 247

Query: 121 KDDKFGLKCQLIPQATLLREAGIRFRKSENKSLENVSFE--KGVLKLPLLIVDDN-TETN 180
             ++  ++CQ I  AT LR+AGI+F KS   +L +VSF+  + VL+LP L +DD  TE  
Sbjct: 248 LTNEDNVECQSIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAIDDILTEPT 307

Query: 181 LLNVMAFEKLH-DVGSQVTSFVVLMNNLIDIDKDVELLSNDNII-ANALGNNEEAANLFS 240
           L+N+MAFEKLH  V  QVTSFVVLM NLI IDK+V LL++  I+ +N + +      LF+
Sbjct: 308 LINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKNVSLLASKGILSSNVIHDGNGVVQLFN 367

Query: 241 VLGKGVSLDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQ--NPWAIISFFGAIFGF 294
           +L KG +  L  N++ E+ +++N + D+ W+ W  NLK    Q  NPW  IS     FGF
Sbjct: 368 LLAKGQTKYL-ENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEFISIAFFTFGF 427


HSP 2 Score: 378.3 bits (970), Expect = 1.3e-101
Identity = 197/249 (79.12%), Postives = 221/249 (88.76%), Query Frame = 1

Query: 58  DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPSTLSYPNMDEIKKNNKDDKF 117
           DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYP TL+YP  DEIK+NNKDD+F
Sbjct: 442 DQNTTLTSLISNLLFVEKDELAIVEKKHILHMYRASLLYPPTLNYPIRDEIKENNKDDEF 501

Query: 118 GLKCQLIPQATLLREAGIRFRKSENKSLENVSFEKGVLKLPLLIVDDNTETNLLNVMAFE 177
           GLKCQ+IPQATLLRE GIRF+KS+NKSLENVSFEKGVL LP LIVDDNT+TNLLNVMAFE
Sbjct: 502 GLKCQIIPQATLLREVGIRFQKSKNKSLENVSFEKGVLTLPSLIVDDNTKTNLLNVMAFE 561

Query: 178 KLHD-VGSQVTSFVVLMNNLIDIDKDVELLSND--NIIANALGNNEEAANLFSVLGKGVS 237
           KLHD VGSQVTSFVVLM+NLI IDKDV+LLSN   NIIANA  N+ +A NLFS LGKGV 
Sbjct: 562 KLHDEVGSQVTSFVVLMHNLIRIDKDVQLLSNGNINIIANAFMNDRDAVNLFSALGKGVY 621

Query: 238 LDLGSNNLTEVHQLVNIHCDDSWNRWWANLKHTYFQNPWAIISFFGA-IFGFAILIVQAV 297
           ++  +NNLT+++  VN +CD SWN+W A+LK +YFQNPW+I+S F A +FGFAILIVQAV
Sbjct: 622 ME--TNNLTKIYISVNQYCDKSWNKWCADLKQSYFQNPWSILSLFHAGVFGFAILIVQAV 681

Query: 298 YQIVDFHTK 303
           YQIVDFHTK
Sbjct: 682 YQIVDFHTK 688

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Y3720_ARATH7.0e-3130.89UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1[more]
Y3264_ARATH4.6e-1429.24Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0K9F5_CUCSA4.8e-13596.46Uncharacterized protein OS=Cucumis sativus GN=Csa_6G089270 PE=4 SV=1[more]
E5GB49_CUCME5.0e-10868.49Putative uncharacterized protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0KAA3_CUCSA9.1e-10279.12Uncharacterized protein OS=Cucumis sativus GN=Csa_6G091270 PE=4 SV=1[more]
A0A0A0KAA3_CUCSA1.8e-3337.82Uncharacterized protein OS=Cucumis sativus GN=Csa_6G091270 PE=4 SV=1[more]
A0A0D2SPR7_GOSRA7.3e-5139.88Uncharacterized protein OS=Gossypium raimondii GN=B456_006G003900 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G50120.12.6e-3932.26 Plant protein of unknown function (DUF247)[more]
AT3G50130.17.0e-3732.83 Plant protein of unknown function (DUF247)[more]
AT3G50140.11.2e-3630.93 Plant protein of unknown function (DUF247)[more]
AT3G50160.15.0e-3530.67 Plant protein of unknown function (DUF247)[more]
AT3G50170.15.5e-3430.09 Plant protein of unknown function (DUF247)[more]
Match NameE-valueIdentityDescription
gi|700191196|gb|KGN46400.1|6.8e-13596.46hypothetical protein Csa_6G089270 [Cucumis sativus][more]
gi|778711703|ref|XP_011656783.1|7.1e-11698.60PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus][more]
gi|307135819|gb|ADN33691.1|7.1e-10868.49hypothetical protein [Cucumis melo subsp. melo][more]
gi|659120434|ref|XP_008460192.1|7.1e-10868.49PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo][more]
gi|659120434|ref|XP_008460192.1|2.1e-4339.94PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004158DUF247_pln
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.349130Cucsa.349130gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.349130.1Cucsa.349130.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.349130.1.CDS.2Cucsa.349130.1.CDS.2CDS
Cucsa.349130.1.CDS.1Cucsa.349130.1.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.349130.1.five_prime_UTR.1Cucsa.349130.1.five_prime_UTR.1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 2..286
score: 2.3
NoneNo IPR availablePANTHERPTHR31549FAMILY NOT NAMEDcoord: 2..296
score: 1.5