Cucsa.302230.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.302230.1 vs. Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.1e-82
Identity = 184/509 (36.15%), Postives = 274/509 (53.83%), Query Frame = 1

Query: 24  STLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPV 83
           +T+I  I   +   L     +RR  LPPGP G P++G LP L G PH  L  +AK YGP+
Sbjct: 11  ATIIYIIVYIIIQKLIATGSWRRRRLPPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPI 70

Query: 84  FKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKK 143
             L++G    VV ++P   K      +  F NR     A   +Y    +VFA  G  WK 
Sbjct: 71  MYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKL 130

Query: 144 LRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFES--VGTQIDIGKVSFLATMKSVVAM 203
           LRK+    M+    L+    VR  E+  ++K +F++  VG +I +  +   A    +  +
Sbjct: 131 LRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQV 190

Query: 204 TWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMG 263
               S RV  E G  +  +F+ M+ EL+ + G  N+ D  P +   DLQGI +  KK+  
Sbjct: 191 ML--SKRVFVEKGKEVN-EFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHK 250

Query: 264 VLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDNSEC-ITNDQLKALLLDIVIGG 323
             D +L    EE         GK  +L  ++  +DN +     ITN  +KALLL++   G
Sbjct: 251 KFDDLLTKMFEEHEATSNERKGKPDFLDFIMANRDNSEGERLSITN--IKALLLNLFTAG 310

Query: 324 TETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRL 383
           T+T+S+ IEWA+ E+M++P   KK ++E+ ++IG N    E   P L YL A+ KE  R 
Sbjct: 311 TDTSSSVIEWALTEMMKNPTIFKKAQQEMDQIIGKNRRFIESDIPNLPYLRAICKEAFRK 370

Query: 384 HPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEE 443
           HP+  L +PR  + + T+ GYYIPK++ +  N+W I RDP +W+NPL+F+PERF+     
Sbjct: 371 HPSTPLNLPRVSSDACTIDGYYIPKNTRLSVNIWAIGRDPDVWENPLEFIPERFLSEKNA 430

Query: 444 ECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSV-ID 503
                 ++    N  E  PFG G+R CAG  +   M+ +IL +L+HSF+W+LP D V I+
Sbjct: 431 ------KIEHRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLIHSFDWKLPNDVVDIN 490

Query: 504 FKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
            +E FG+  +K  PL AI TP LS  D+Y
Sbjct: 491 MEETFGLALQKAVPLEAIVTPRLS-FDIY 507

BLAST of Cucsa.302230.1 vs. Swiss-Prot
Match: C76B1_HELTU (7-ethoxycoumarin O-deethylase OS=Helianthus tuberosus GN=CYP76B1 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.1e-78
Identity = 178/501 (35.53%), Postives = 271/501 (54.09%), Query Frame = 1

Query: 26  LILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFK 85
           +I+   L  + L+W+    +  +LPPGP  LP++G L  L   PH  L  LAKI+GP+  
Sbjct: 5   IIVSTLLLSYILIWVLGVGKPKNLPPGPTRLPIIGNLHLLGALPHQSLAKLAKIHGPIMS 64

Query: 86  LRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLR 145
           L+LG    +V++S    +E    Q+  F  RN        ++    I F     +W+ LR
Sbjct: 65  LQLGQITTLVISSATAAEEVLKKQDLAFSTRNVPDAVRAYNHERHSISFLHVCTEWRTLR 124

Query: 146 KIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV--GTQIDIGKVSFLATMKSVVAMTW 205
           +I    + S S+L+A   +R ++V ++I    ++      + IG+ +F  ++  +    +
Sbjct: 125 RIVSSNIFSNSSLEAKQHLRSKKVEELIAYCRKAALSNENVHIGRAAFRTSLNLLSNTIF 184

Query: 206 GDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVL 265
                   ED  G E  FRE++  ++V     N+ DVFPVL + D QGI R   +    +
Sbjct: 185 SKDLTDPYEDS-GKE--FREVITNIMVDSAKTNLVDVFPVLKKIDPQGIKRGMARHFSKV 244

Query: 266 DGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTET 325
            GI +  IEE+ + G     +G  L + L++   +DN     +  +KAL LD+ + GT+T
Sbjct: 245 LGIFDQLIEERMRTGRFE--QGDVLDVCLKMM--QDNPNEFNHTNIKALFLDLFVAGTDT 304

Query: 326 TSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRLHPA 385
           TS TIEWAM EL++ P  M K KEEL+KVIG  +IV+E    +L YL+ ++KE LRLHP 
Sbjct: 305 TSITIEWAMTELLRKPHIMSKAKEELEKVIGKGSIVKEDDVLRLPYLSCIVKEVLRLHPP 364

Query: 386 IFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEEECA 445
             LL+PR + +   L GY IP  + ++ N W I RDPT+WD+ L+F P+RF++S  +   
Sbjct: 365 SPLLLPRKVVTQVELSGYTIPAGTLVFVNAWAIGRDPTVWDDSLEFKPQRFLESRLDVRG 424

Query: 446 GQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWEL----PKDSVID 505
             F+L          PFG G+R C GI LA RM+  +L SLL++F+W++    P D V+D
Sbjct: 425 HDFDL---------IPFGAGRRICPGIPLATRMVPIMLGSLLNNFDWKIDTKVPYD-VLD 484

Query: 506 FKEKFGIVNKKLNPLVAIPTP 521
             EK G    K  PL  +P P
Sbjct: 485 MTEKNGTTISKAKPLCVVPIP 488

BLAST of Cucsa.302230.1 vs. Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.0e-77
Identity = 174/500 (34.80%), Postives = 270/500 (54.00%), Query Frame = 1

Query: 26  LILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFK 85
           + L   +F+  LL + +  R   LPPGPRG P++G LP L   PH  L  +AK YG +  
Sbjct: 13  IFLTTHIFISTLLSITNGRR---LPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMY 72

Query: 86  LRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLR 145
           L++G    VV ++P   K      +  F NR     A   +YG   +VFA  G  WK LR
Sbjct: 73  LKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWKLLR 132

Query: 146 KIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV--GTQIDIGKVSFLATMKSVVAMTW 205
           K+    M+    L+    VR  E+  ++K +F+    G ++ + ++   A    +  +  
Sbjct: 133 KLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQVIL 192

Query: 206 GDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVL 265
             S RV    GV +  +F++M+ EL+   G  N+ D  P L   DLQGI +  K++    
Sbjct: 193 --SKRVFVNKGVEVN-EFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRLHKKF 252

Query: 266 DGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTET 325
           D +L    +E +       GK  +L  ++E +DN +  E ++   +KALLL++   GT+T
Sbjct: 253 DALLTKMFDEHKATSYERKGKPDFLDCVMENRDNSEG-ERLSTTNIKALLLNLFTAGTDT 312

Query: 326 TSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRLHPA 385
           +S+ IEWA+AE+M++P  +KK + E+ +VIG N  + E   P L YL A+ KET R HP+
Sbjct: 313 SSSAIEWALAEMMKNPAILKKAQGEMDQVIGNNRRLLESDIPNLPYLRAICKETFRKHPS 372

Query: 386 IFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEEECA 445
             L +PR       + GYYIPK++ +  N+W I RDP +W+NPL+F PERF+        
Sbjct: 373 TPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPEVWENPLEFYPERFLSGRNS--- 432

Query: 446 GQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSV-IDFKE 505
              ++    N  E  PFG G+R CAG  +   M+ +IL +L+HSF+W+LP + + ++ +E
Sbjct: 433 ---KIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEVIELNMEE 492

Query: 506 KFGIVNKKLNPLVAIPTPSL 523
            FG+  +K  PL A+ TP L
Sbjct: 493 AFGLALQKAVPLEAMVTPRL 499

BLAST of Cucsa.302230.1 vs. Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.3e-77
Identity = 168/511 (32.88%), Postives = 283/511 (55.38%), Query Frame = 1

Query: 22  QISTLILFIFLFMFALLW-LRSKFRRP---SLPPGPRGLPLVGYLPFLSGNPHHKLTHLA 81
           +I +LIL+  +F F L + LRS FR+     LPPGP+  P++G L  L   PH     +A
Sbjct: 2   EILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMA 61

Query: 82  KIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQD 141
           + YGP+  L++G    VV  S +   +     +  F +R     A   +Y    +VFA  
Sbjct: 62  QTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPY 121

Query: 142 GEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKS 201
           G  W+ LRKI    + S   LD    VR+ EV+ + + +  +    + +G++  + T  +
Sbjct: 122 GPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNA 181

Query: 202 VVAMTWGDSGRVMGEDGVGLEV-KFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRT 261
           +  +  G      G   V  +  +F+ M+ E++V+ G  N+ D  P L   D+QG+  + 
Sbjct: 182 LARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKM 241

Query: 262 KKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDNSEC--ITNDQLKALLL 321
           KK+    D  L   +EE +  G   G     L  L+ L++++ +++   +T+ ++KALLL
Sbjct: 242 KKLHARFDAFLTDILEEHK--GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLL 301

Query: 322 DIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVI 381
           ++ + GT+T+S+T+EWA+AEL+++P  + + ++E+ KV+G + +V E    +L YL A++
Sbjct: 302 NLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIV 361

Query: 382 KETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERF 441
           KET RLHP+  L +PR  + S  + GY+IPK ST+  N+W I RDP  W +PL+F PERF
Sbjct: 362 KETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERF 421

Query: 442 VKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPK 501
           +   E+      ++    N  E  PFG G+R CAG+ L  RM+  ++A+L+H+F W+L  
Sbjct: 422 LPGGEKP-----KVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVS 481

Query: 502 ---DSVIDFKEKFGIVNKKLNPLVAIPTPSL 523
                +++ +E +G+  ++ +PLV  P P L
Sbjct: 482 GQLPEMLNMEEAYGLTLQRADPLVVHPRPRL 505

BLAST of Cucsa.302230.1 vs. Swiss-Prot
Match: C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.9e-77
Identity = 178/519 (34.30%), Postives = 274/519 (52.79%), Query Frame = 1

Query: 24  STLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPV 83
           ++L++ + LF+F     R   RRP  PPGPRG P++G +  +    H  L +LAK YG +
Sbjct: 17  TSLVIVVSLFIFISFITRR--RRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGL 76

Query: 84  FKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKK 143
             LR+G      ++SP   ++    Q+++F NR  T+     +Y  + + FA  G  W++
Sbjct: 77  CHLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQ 136

Query: 144 LRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTW 203
           +RK+ V K+ S+   + S+   R EV K+++ V  +VG  I++G+  F  T        +
Sbjct: 137 MRKVCVMKVFSRKRAE-SWASVRDEVDKMVRSVSCNVGKPINVGEQIFALTRNITYRAAF 196

Query: 204 GDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVL 263
           G S    G+D      +F  ++ E   L G+ NV+D  P  G  D QGI +R  K    L
Sbjct: 197 G-SACEKGQD------EFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDL 256

Query: 264 DGILNSAIEE--QRKMGGNGGGKGGYLQL-----LLELQDNE----------DNSECITN 323
           DG ++  I+E  ++K   N    G  +       LL     E           NS  +T 
Sbjct: 257 DGFIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTR 316

Query: 324 DQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPK 383
           D +KA+++D++ GGTET ++ IEWA+ EL++ P+ +K+V++EL +V+GL+  VEE    K
Sbjct: 317 DNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEK 376

Query: 384 LCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNP 443
           L YL   +KETLR+HP I LL+  T    T++ G++IPK S +  N + I RDPT W +P
Sbjct: 377 LTYLKCTLKETLRMHPPIPLLLHET-AEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDP 436

Query: 444 LKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLH 503
             F P RF++    +  G        +  EF PFG G+RSC G+ L    L   +A +LH
Sbjct: 437 DTFRPSRFLEPGVPDFKG--------SNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILH 496

Query: 504 SFEWELP---KDSVIDFKEKFGIVNKKLNPLVAIPTPSL 523
            F W+LP   K S +D  + FG+   K   L A+PT  L
Sbjct: 497 CFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of Cucsa.302230.1 vs. TrEMBL
Match: A0A0A0KMT1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G158600 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 7.0e-158
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 1

Query: 34  MFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLC 93
           MFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLC
Sbjct: 1   MFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLC 60

Query: 94  VVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMI 153
           VVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMI
Sbjct: 61  VVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMI 120

Query: 154 SKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGED 213
           SKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGED
Sbjct: 121 SKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGED 180

Query: 214 GVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEE 273
           GVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEE
Sbjct: 181 GVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEE 240

Query: 274 QRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLL 315
           QRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLL
Sbjct: 241 QRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLL 281

BLAST of Cucsa.302230.1 vs. TrEMBL
Match: M5WDL9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019218mg PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 8.9e-145
Identity = 256/508 (50.39%), Postives = 354/508 (69.69%), Query Frame = 1

Query: 28  LFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLR 87
           + + LF F   W  SK   P LPPGPRGLP +G LP L  N HH+ ++LA IYGP++KL+
Sbjct: 8   MVVALFWFLWNWKSSKNPMPPLPPGPRGLPFLGSLPLLGPNLHHEFSNLATIYGPIYKLQ 67

Query: 88  LGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKI 147
           LG KLC VL+SP+ +K+    Q+TLF NR+ T+ A ++SYGC+ I F   G  W++LRK+
Sbjct: 68  LGSKLCFVLSSPSIVKQVLRDQDTLFANRDPTIAAQIASYGCTDIAFGPYGPGWRRLRKV 127

Query: 148 FVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSG 207
           F+  M+SK+NLDASY +R++EV K I  V++ VG+ ID+G+ +FL ++ +V+ M WG  G
Sbjct: 128 FMSHMLSKANLDASYALRKEEVLKSISHVYDKVGSPIDLGQFAFLTSINTVMRMLWG--G 187

Query: 208 RVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGIL 267
            +  E+G  L  +FR ++ E++ +LG  NVSD    L RFD+QGI RR K++  + + I 
Sbjct: 188 TLEAENGADLGAEFRNVVAEIIEVLGKPNVSDFLTSLARFDVQGIARRMKQLQSMTEKIF 247

Query: 268 NSAIEEQRKMGGNGGG-------KGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGG 327
           +SAIE Q+               +  +LQ+LLEL D+ED ++ IT  QLKALLLDIV+GG
Sbjct: 248 DSAIERQKSEAVEKDEGLELKHERKDFLQILLELNDHEDGAKSITLQQLKALLLDIVVGG 307

Query: 328 TETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRL 387
           T+TT+T +EW MAEL++H D +KKV+EELK+V+GL  +VEE H PKL YL+AVIKET RL
Sbjct: 308 TDTTATMVEWVMAELIKHQDDLKKVQEELKEVVGLKNMVEESHIPKLHYLDAVIKETSRL 367

Query: 388 HPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEE 447
           HPA+ LL P   + STT+GGY IPK STI+ N+W I RDP +WDNPL+F P+RF+    +
Sbjct: 368 HPALPLLAPHCPSQSTTIGGYNIPKGSTIFINIWAIHRDPNVWDNPLEFRPKRFL---ND 427

Query: 448 ECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDF 507
             +  F+     N +E+ PFG G+R CAG+ LAE+M++++LAS LHSFEW LP D+  D 
Sbjct: 428 PSSNNFDY--KGNKLEYLPFGSGRRMCAGLPLAEKMMIYVLASFLHSFEWRLPTDAKHDL 487

Query: 508 KEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           ++KFGIV KK+ PL+ IPTP LS  +LY
Sbjct: 488 QDKFGIVTKKMTPLIVIPTPRLSKLELY 508

BLAST of Cucsa.302230.1 vs. TrEMBL
Match: M5WSD8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019849mg PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 3.0e-140
Identity = 252/513 (49.12%), Postives = 346/513 (67.45%), Query Frame = 1

Query: 23  ISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGP 82
           IS + + +F FM+   W  SK   P LP GPRGLP +G LPFL  N H + T+LA +YGP
Sbjct: 27  ISAMAVALFWFMWN--WKSSKNPTPPLPSGPRGLPFLGSLPFLGPNLHREFTNLASVYGP 86

Query: 83  VFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWK 142
           ++KL+LG KLC VL+SP+ +K+    Q+TLF N +  + A + SYG + I F   G DW+
Sbjct: 87  IYKLQLGSKLCFVLSSPSLVKQVVRDQDTLFANHDPIIAAQIISYGGTDIAFGSYGPDWR 146

Query: 143 KLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMT 202
           +LRK+FV  ++SK NLDA Y +R++EV K I  ++    + ID G+V+F   + +++ M 
Sbjct: 147 RLRKVFVSHVMSKGNLDACYALRKEEVLKSIDHIYGKSDSPIDFGQVAFFTAVNTIMRML 206

Query: 203 WGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGV 262
           WG  G +  E G  L  + R ++ E+V LLG  N+SD FP L +FD+QGI RR K+V+ V
Sbjct: 207 WG--GTLEAEKGTDLGAELRNVVAEMVELLGKGNISDFFPWLAKFDVQGIARRAKQVLSV 266

Query: 263 LDGILNSAIEEQRKMGGNGGG-------KGGYLQLLLELQDNEDNSECITNDQLKALLLD 322
            + ILNSAIE+Q        G       + G+LQ LLEL ++ D++E +T  Q+KALL D
Sbjct: 267 TEKILNSAIEKQMSEAAEQDGGLSLKHERKGFLQFLLELNEHGDDAESLTLQQIKALLTD 326

Query: 323 IVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIK 382
           I+ GGT+TT+T +EW MAELMQ PD +KKV+EELK+V+GL  +VEE H PKL YL+AVIK
Sbjct: 327 IMAGGTDTTATMMEWVMAELMQQPDDLKKVQEELKEVVGLKNLVEESHIPKLHYLDAVIK 386

Query: 383 ETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFV 442
           ET RLHPA+ LL P   + STT+GG+ IPK S+++ N+W I RDP +WDNPL+F P+RF+
Sbjct: 387 ETSRLHPALPLLAPHCPSQSTTIGGFKIPKGSSVFLNVWAIHRDPNVWDNPLEFRPKRFL 446

Query: 443 KSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKD 502
                       +  + N +E+ PFG G+R C GI LAERM++++LAS LHSFEW LP D
Sbjct: 447 NDPPTN-----NIHYNGNKLEYLPFGSGRRMCPGIPLAERMMIYVLASFLHSFEWRLPND 506

Query: 503 SVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           + +D ++KFGIV KK+ PLV IPTP LS  +LY
Sbjct: 507 AKLDLQDKFGIVTKKMTPLVVIPTPRLSKLELY 530

BLAST of Cucsa.302230.1 vs. TrEMBL
Match: V4UAL4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014861mg PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 6.2e-138
Identity = 261/535 (48.79%), Postives = 365/535 (68.22%), Query Frame = 1

Query: 15  VDSDNQNQ---ISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNP-H 74
           VD  + N+   I+ L+  + +  FA    +S+     LPPGPRGLP+VGYLPFL     H
Sbjct: 5   VDDASNNEAAIITCLVSMLAVVYFAWNVNKSRKANAKLPPGPRGLPVVGYLPFLGTTDLH 64

Query: 75  HKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCS 134
            K T L+ +YGP+FKL LG KLCVV++SP+ +K+    Q+T F +R+  +  L++S+G +
Sbjct: 65  KKFTELSGVYGPIFKLWLGNKLCVVVSSPSLVKQVVRDQDTTFADRDPPISGLVTSFGGN 124

Query: 135 GIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFES---VGTQIDIG 194
            I ++  G +W+KLRK FV KM+S ++LDA Y +R+ EV+  I+ ++ +   +G  IDIG
Sbjct: 125 DITWSNYGPEWRKLRKFFVGKMMSNASLDACYALRKHEVKNTIRDLYNNGNKIGKPIDIG 184

Query: 195 KVSFLATMKSVVAMTWGDSGRVM--GEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLG 254
           ++S    + ++  M WG++  +   G   +G+E+KF+    EL+VLLG+ N+SD+FP+L 
Sbjct: 185 ELSIATLVCAMQNMLWGEALELKEKGTTNLGVELKFK--FAELMVLLGTPNISDIFPMLS 244

Query: 255 RFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKG------------GYLQLLLE 314
             D+QGI RR KK+    + I+NS +E+ R    N  GK              +LQLLLE
Sbjct: 245 WLDIQGIERRAKKISLWFEKIINSTVEKYRSKDFNVEGKERAGENFEGSRNKNFLQLLLE 304

Query: 315 LQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVI 374
           LQ+NED S  I+ +Q KA+L+DI+  GT+TT+T +EW MAELMQHP  MKKV+EEL +V+
Sbjct: 305 LQENEDGSSSISMNQFKAVLVDIITAGTDTTTTMVEWTMAELMQHPQIMKKVQEELAQVV 364

Query: 375 GLNAIVEEFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNL 434
           G+++ VEEFH PKL YL+AV+KE+ RLHPA+ LL+PR  + S ++GGY IPKD+TI  N+
Sbjct: 365 GMDSCVEEFHLPKLKYLDAVVKESSRLHPAVPLLLPRRASESGSIGGYNIPKDTTIMLNV 424

Query: 435 WGIQRDPTIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALA 494
           W I RDP +WDNPL+F PERF+   ++  A +F+   S N  ++ PFG G+R CAGI LA
Sbjct: 425 WEIHRDPKLWDNPLQFRPERFL---DDGIASKFDY--SGNNFQYLPFGSGRRMCAGIPLA 484

Query: 495 ERMLMFILASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           ERMLMF+LASLLHSFEWELP  + ++  EKFGIV KK  PLVAIPTP LSNS+LY
Sbjct: 485 ERMLMFVLASLLHSFEWELPAGTKLNLSEKFGIVIKKKEPLVAIPTPRLSNSELY 532

BLAST of Cucsa.302230.1 vs. TrEMBL
Match: M5WBY3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019443mg PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-137
Identity = 250/506 (49.41%), Postives = 333/506 (65.81%), Query Frame = 1

Query: 30  IFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLG 89
           + LF F   W  SK   P LPPGPRGLP +G LPFL  N HH+ T+LA +YGP++KL+LG
Sbjct: 3   VALFWFMWNWKSSKNPTPPLPPGPRGLPFLGSLPFLGPNLHHEFTNLASVYGPIYKLQLG 62

Query: 90  PKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFV 149
            KLC VL+SP+ +K+    Q+TLF N + T+ A ++SYG + I F   G DW+KLRK+FV
Sbjct: 63  SKLCFVLSSPSLVKQVVRDQDTLFANHDPTIAAQIASYGGTDIAFGSYGPDWRKLRKVFV 122

Query: 150 RKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGKVSFLATMKSVVAMTWGDSGRV 209
             ++SK NLDA Y +R++EV K I  +F   GT ID G+V+F   + +++ M WG  G +
Sbjct: 123 SHVMSKGNLDACYALRKEEVLKSIGHIFGKSGTPIDFGQVAFFTAINTIMRMLWG--GTL 182

Query: 210 MGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNS 269
             E G  L  + R ++ E+V LLG  N+SD FP L RFD                 ILNS
Sbjct: 183 EAEKGTDLGAELRNVVAEMVELLGKGNISDFFPWLARFD-----------------ILNS 242

Query: 270 AIEEQRKMGGNGGG-------KGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTE 329
           AIE+Q        G       + G+LQ LLEL ++ + +E +T  Q+KALL DIV GGT+
Sbjct: 243 AIEKQMSEAAEQDGGLSLKHERKGFLQFLLELNEHGNGAESLTLQQIKALLTDIVAGGTD 302

Query: 330 TTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRLHP 389
           TT+T +EW MAELMQHPD +KKV++ELK+V+GL+ +VEE H PKL YL+ VIKET RLHP
Sbjct: 303 TTATMVEWVMAELMQHPDDLKKVQQELKEVVGLDNLVEESHVPKLHYLDVVIKETSRLHP 362

Query: 390 AIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEEEC 449
           A  LLVP   + STT+GG+ IPK S ++ N+W I RDP +WDNPL+F P+RF+       
Sbjct: 363 AFPLLVPHCPSQSTTIGGFKIPKGSNVFLNVWAIHRDPNVWDNPLEFRPKRFLNDPPTN- 422

Query: 450 AGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDFKE 509
                +  + N +E+ PFG G+R C GI LAERM++++LAS LHSFEW LP D+ +D ++
Sbjct: 423 ----NIHYNGNKLEYLPFGSGRRMCPGIPLAERMMIYVLASFLHSFEWRLPNDAKLDLQD 482

Query: 510 KFGIVNKKLNPLVAIPTPSLSNSDLY 529
           KFGIV KK+ PLV IPTP LS  +LY
Sbjct: 483 KFGIVTKKMTPLVVIPTPRLSKLELY 484

BLAST of Cucsa.302230.1 vs. TAIR10
Match: AT4G12300.1 (AT4G12300.1 cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 430.6 bits (1106), Expect = 1.4e-120
Identity = 228/517 (44.10%), Postives = 336/517 (64.99%), Query Frame = 1

Query: 18  DNQNQISTLILFIFLFMFALLW-LRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHL 77
           DN   ++   + I   +F++LW +  +  +PSLPPGPRGLP+VG LPFL  + H    +L
Sbjct: 10  DNTINLTPYAIVILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANL 69

Query: 78  AKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQ 137
           A+ +GP+FKL LG KL +V+ SP+  +E    Q+  F NR+  +    ++YG   IV+  
Sbjct: 70  AQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTP 129

Query: 138 DGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQ--IDIGKVSFLAT 197
            G +W++LRKI V K++S+  LD+ Y +RR+EVR+  + ++E    Q  + +G   FL  
Sbjct: 130 YGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTM 189

Query: 198 MKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGR 257
           M   + M WG S +    + VG E  F+ ++ E+  LL   NVSD FP L RFDLQG+ +
Sbjct: 190 MNLTMNMLWGGSVKAEEMESVGTE--FKGVISEITRLLSEPNVSDFFPWLARFDLQGLVK 249

Query: 258 RTKKVMGVLDGILNSAIEEQRKMGGNGGGK-GGYLQLLLELQDNEDNSEC-ITNDQLKAL 317
           R       LD +L+ AIE+ + + G    +   +LQ L++L+D E +SE  IT + +KAL
Sbjct: 250 RMGVCARELDAVLDRAIEQMKPLRGRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKAL 309

Query: 318 LLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNA 377
           L D+V+GGT+T++ TIE+AMAELM +P+ +K+ +EEL +V+G + IVEE H  +L Y+ A
Sbjct: 310 LTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILA 369

Query: 378 VIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPE 437
           ++KETLRLHP + LLVP     +T +GGY IPKD+ I+ N+W IQRDP +W+NP +F PE
Sbjct: 370 IMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPE 429

Query: 438 RFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWEL 497
           RF+ +N  +  G            ++PFG G+R CAG+ALAERM+++ LA+LLHSF+W++
Sbjct: 430 RFLDNNSCDFTGA--------NYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKI 489

Query: 498 PKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYL 530
           P+  V+D KEKFGIV K   PLVA+P P  S+S+LYL
Sbjct: 490 PEGHVLDLKEKFGIVLKLKIPLVALPIPRFSDSNLYL 516

BLAST of Cucsa.302230.1 vs. TAIR10
Match: AT4G12320.1 (AT4G12320.1 cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 418.7 bits (1075), Expect = 5.4e-117
Identity = 221/518 (42.66%), Postives = 336/518 (64.86%), Query Frame = 1

Query: 19  NQNQISTLILFIFLFMFALLW-LRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLA 78
           N   ++   + I + +F++LW L  +  +P LPPGPRGLP+VG LPFL  + H   T LA
Sbjct: 11  NTINLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLA 70

Query: 79  KIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQD 138
           + YGP+FKL LG KL VV+ +P+  +E    Q+  F N +  + A   +YG   +V+   
Sbjct: 71  ESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPY 130

Query: 139 GEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQ--IDIGKVSFLATM 198
           G +W+ LRK+ V K++S   L++ Y +RR+E+R+  + +++    +  +++G+  FL  M
Sbjct: 131 GAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMM 190

Query: 199 KSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRR 258
              + M WG S +    + VG E  F+E++ E+  LLG  NVSD FP L RFDLQG+ ++
Sbjct: 191 NLTMNMLWGGSVKAEEMESVGTE--FKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKK 250

Query: 259 TKKVMGVLDGILNSAIEEQ---RKMGGNGGGKGGYLQLLLELQDNEDNSEC-ITNDQLKA 318
                  LD IL+ AIE+    R   G+ G    +LQ L++L+D E +SE  IT + +KA
Sbjct: 251 MHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKA 310

Query: 319 LLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLN 378
           +L+D+V+GGT+T++ TIE+AMAEL++ P+ MK+ ++EL +V+G + I+EE H  +L +++
Sbjct: 311 VLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFIS 370

Query: 379 AVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMP 438
           A++KETLRL+P I LLVP   + +  +GGY IPK++ I+ N+W IQRDP +W+ P +F P
Sbjct: 371 AIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRP 430

Query: 439 ERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWE 498
           ERF+     +  G            + PFG G+R CAGIALAERM+++ LA+LLHSF+W+
Sbjct: 431 ERFLDKKSCDFTG--------TDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWK 490

Query: 499 LPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYL 530
           +P+  ++D KEKFGIV K  +PLVA+P P LSNS+LYL
Sbjct: 491 IPEGHILDLKEKFGIVLKLKSPLVALPVPRLSNSNLYL 518

BLAST of Cucsa.302230.1 vs. TAIR10
Match: AT4G12310.1 (AT4G12310.1 cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 406.0 bits (1042), Expect = 3.6e-113
Identity = 215/508 (42.32%), Postives = 323/508 (63.58%), Query Frame = 1

Query: 28  LFIFLFMFALLW-LRSKFRRPSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKL 87
           + I    F++LW +  +  +P LPPGPRGLP+VG LPFL  + H   T LA+ +GP+FKL
Sbjct: 22  VLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKL 81

Query: 88  RLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRK 147
            LG KL VV+ SP+   E    Q+  F N +  + A   +YG   +V+   G +W+ LRK
Sbjct: 82  NLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRK 141

Query: 148 IFVRKMISKSNLDASYCVRRQEVRKVIKGVFES--VGTQIDIGKVSFLATMKSVVAMTWG 207
           +   K+ S+  LD+ Y +RR+E+R+  + +++     + +++G+  FL  M  ++ M WG
Sbjct: 142 VCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWG 201

Query: 208 DSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLD 267
             G V  ED   +  +F+ ++ E+  LLG  NVSD FP+L RFDLQG+ ++       LD
Sbjct: 202 --GSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLD 261

Query: 268 GILNSAIEEQRKMGGNGGGKG---GYLQLLLELQDNEDNSEC-ITNDQLKALLLDIVIGG 327
            IL+ AIE+ +++    G  G    +LQ L++L+D E +S+  IT + +KA+L+D+V+GG
Sbjct: 262 AILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMVVGG 321

Query: 328 TETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRL 387
           TE+++ TIE+ MAEL+ +P+ M++ ++EL +V+G + IVEE H   L Y+ AV+KETLRL
Sbjct: 322 TESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKETLRL 381

Query: 388 HPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEE 447
           +P I LLVP   + +  +GGY IPK++ I+ N+W IQRDP +W+ P +F PERF+     
Sbjct: 382 YPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFLDKKSC 441

Query: 448 ECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDF 507
           +  G            + PFG G+R CAGIALAERM+++ LA+LLHSF+W +P   V+D 
Sbjct: 442 DFTG--------TDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHVLDL 501

Query: 508 KEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           +EKFGIV K   PLVA+P P LSNS+ Y
Sbjct: 502 EEKFGIVLKLKTPLVALPIPRLSNSNFY 519

BLAST of Cucsa.302230.1 vs. TAIR10
Match: AT4G12330.1 (AT4G12330.1 cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 394.8 bits (1013), Expect = 8.3e-110
Identity = 211/529 (39.89%), Postives = 332/529 (62.76%), Query Frame = 1

Query: 9   LFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRR--PSLPPGPRGLPLVGYLPFLS 68
           +F F+++ + N +    L   +   +F  +W+  K +R  P LPPGPRGLP+VG LPFL 
Sbjct: 4   IFTFFTIQNRNDHFDFVLATIVISSVFWYIWVYVKSKRLFPPLPPGPRGLPIVGNLPFLH 63

Query: 69  GNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSS 128
              H     LA+ +GPVFKL LG KL +V+TS    ++     + +F N +  V   LS+
Sbjct: 64  PELHTYFHSLAQKHGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLST 123

Query: 129 YGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASY--CVRRQEVRKVIKGVFESV--GT 188
           YG   IV++  G +W  LRKI + KM+S + LD++    +RRQE R+ ++ + +    G 
Sbjct: 124 YGGVDIVWSPYGPEWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGL 183

Query: 189 QIDIGKVSFLATMKSVVAMTWGDSGRVMGE-DGVGLEVKFREMMDELVVLLGSRNVSDVF 248
            +++G+  F+  +  V  M WG++     E + VG E  F E++ E++ ++G  NVSD F
Sbjct: 184 AVNVGEQIFVTILNVVTQMLWGETVADDEEREKVGAE--FLELITEIIDVVGKPNVSDFF 243

Query: 249 PVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGG-YLQLLLELQDNEDN 308
           PVL RFDLQG+ +R ++    +D + +  I ++  M     G GG +L +LL  +D ++N
Sbjct: 244 PVLSRFDLQGLAKRVRRSAQRMDRMFDRIISQRMGMDKGSKGNGGDFLMVLLNAKDEDEN 303

Query: 309 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 368
              ++ + +KALL+D+V+GGT+T+  TIE+AMAEL+   + MK+ ++EL KV+G N IVE
Sbjct: 304 ---MSMNHVKALLMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIVE 363

Query: 369 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 428
           E H  KL Y+ +++KETLRLHPA+ LL+PR  + +T +GGY IP DS ++ N+W I R+P
Sbjct: 364 EKHITKLPYILSIMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRNP 423

Query: 429 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI 488
            +W+NPL+F P+RF+    +          S N   ++PFG G+R CAG+A+AE+++++ 
Sbjct: 424 NVWENPLEFNPDRFLDKGYD---------FSGNDYSYFPFGSGRRICAGMAMAEKVVLYN 483

Query: 489 LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYL 530
           LA+LLHSF+W + +   ++ +EKFGI+ K  NPLVA P   LS+ +L+L
Sbjct: 484 LATLLHSFDWRIGEGEKVELEEKFGILLKLKNPLVATPVLRLSDPNLFL 518

BLAST of Cucsa.302230.1 vs. TAIR10
Match: AT5G44620.1 (AT5G44620.1 cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-103
Identity = 200/498 (40.16%), Postives = 314/498 (63.05%), Query Frame = 1

Query: 38  LWLRSKFRR--PSLPPGPRGLPLVGYLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVV 97
           LWL +K +R  P LPPGP GLP++G LPFL    H     LAK +GP+FKL LG KL +V
Sbjct: 33  LWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIV 92

Query: 98  LTSPAFIKEAFHHQETLFPNRNTTVCALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISK 157
           +TS    +E     + +F N +      +++YG + I+++  G  W+ LRK+ V +++  
Sbjct: 93  VTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRN 152

Query: 158 SNLDASYCVRRQEVRKVIKGVFES--VGTQIDIGKVSFLATMKSVVAMTWGDSGRVMGED 217
           + LD+S  +RR+E R+ ++ + +   VG+ +++G+  FL  +  V  M WG + +    +
Sbjct: 153 AMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEERE 212

Query: 218 GVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEE 277
            VG E  F E++ E+  LL   N+SD FPVL RFDLQG+ +R ++    +D + +  I +
Sbjct: 213 VVGAE--FLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQ 272

Query: 278 QRKMG--GNGGGKGGYLQLLLELQDNEDNSECITNDQLKALLLDIVIGGTETTSTTIEWA 337
           +  M    + G    +L +LL+++D E     +T + +KA+L+D+V+GGT+T+   IE+A
Sbjct: 273 RLGMDRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLMDMVLGGTDTSLHVIEFA 332

Query: 338 MAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLCYLNAVIKETLRLHPAIFLLVPRT 397
           MAEL+ +PD MK+ ++E+ KV+G   +VEE H  KL Y+ A++KETLRLH    LLVPR 
Sbjct: 333 MAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYILAIMKETLRLHTVAPLLVPRR 392

Query: 398 LTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLKFMPERFVKSNEEECAGQFELGDS 457
            + +T +GG+ IPKDS I+ N W I R+P +W+NPLKF P+RF+     + +  F+  D 
Sbjct: 393 PSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFDPDRFL-----DMSYDFKGNDF 452

Query: 458 NNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSFEWELPKDSVIDFKEKFGIVNKKL 517
           N    + PFG G+R C G+A+ ER++++ LA+ LHSF+W++P+   ++ +EKFGIV +  
Sbjct: 453 N----YLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQGERVEVEEKFGIVLELK 512

Query: 518 NPLVAIPTPSLSNSDLYL 530
           NPLVA P   LS+ +LYL
Sbjct: 513 NPLVATPVLRLSDPNLYL 519

BLAST of Cucsa.302230.1 vs. NCBI nr
Match: gi|449459750|ref|XP_004147609.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis sativus])

HSP 1 Score: 1067.4 bits (2759), Expect = 8.2e-309
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 1

Query: 1   MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG 60
           MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG
Sbjct: 1   MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG 60

Query: 61  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV 120
           YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV
Sbjct: 61  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV 120

Query: 121 CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV 180
           CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV
Sbjct: 121 CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV 180

Query: 181 GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV 240
           GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV
Sbjct: 181 GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV 240

Query: 241 FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN 300
           FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN
Sbjct: 241 FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN 300

Query: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 360
           SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE
Sbjct: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 360

Query: 361 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 420
           EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP
Sbjct: 361 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 420

Query: 421 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI 480
           TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI
Sbjct: 421 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI 480

Query: 481 LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYLA 531
           LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYLA
Sbjct: 481 LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYLA 530

BLAST of Cucsa.302230.1 vs. NCBI nr
Match: gi|659073592|ref|XP_008437145.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 883.6 bits (2282), Expect = 1.7e-253
Identity = 447/529 (84.50%), Postives = 473/529 (89.41%), Query Frame = 1

Query: 4   LSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLP 63
           LSF  L  FWS   +  NQIS  IL +FL +  L   R KF RP LPPGP GLPLVGYLP
Sbjct: 38  LSFSILPSFWSTYFNENNQISISILLVFLVL-TLFRFRPKFWRPCLPPGPCGLPLVGYLP 97

Query: 64  FLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCAL 123
           FLSGNPHHKLTHLAKIYGPV+KLRLGPKLC+VL SPA +KEAFHHQETLFPNR+TTVCAL
Sbjct: 98  FLSGNPHHKLTHLAKIYGPVYKLRLGPKLCIVLNSPASVKEAFHHQETLFPNRDTTVCAL 157

Query: 124 LSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQ 183
           LS YG SGIVFAQD  DWKKLRKIF RKMISKSNLDASY VRRQEVRKVIKGVFESVGTQ
Sbjct: 158 LSGYGGSGIVFAQDEGDWKKLRKIFTRKMISKSNLDASYFVRRQEVRKVIKGVFESVGTQ 217

Query: 184 IDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPV 243
           IDIGKVSF ATMKSV+AMTWGDSGR+MGEDGVGLE KFREMMDEL+VLLGS NVSDVFPV
Sbjct: 218 IDIGKVSFHATMKSVLAMTWGDSGRLMGEDGVGLEEKFREMMDELMVLLGSPNVSDVFPV 277

Query: 244 LGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMG--GNGGGKGGYLQLLLELQDNEDNS 303
           LGRFDLQGIGRRTKKVMGVLDGILN +IEEQRKMG  GNG GKGGYLQLLLELQDNEDNS
Sbjct: 278 LGRFDLQGIGRRTKKVMGVLDGILNFSIEEQRKMGGKGNGEGKGGYLQLLLELQDNEDNS 337

Query: 304 ECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEE 363
           ECITNDQLKALLLDIVIGGTETT+TT+EWAMAELMQHP+T+KKVKEELKKVIGLNA+VEE
Sbjct: 338 ECITNDQLKALLLDIVIGGTETTATTVEWAMAELMQHPNTIKKVKEELKKVIGLNAVVEE 397

Query: 364 FHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPT 423
           FHF KLCYLNAVIKETLRLHP IFLLVPR+LTSSTTLGGYYIPKDST+YFNLW IQRDP 
Sbjct: 398 FHFSKLCYLNAVIKETLRLHPPIFLLVPRSLTSSTTLGGYYIPKDSTLYFNLWAIQRDPK 457

Query: 424 IWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFIL 483
            WDNP KFMPERF+ +N            ++NAMEFYPFGYGK+SCAG+ALAERMLMFIL
Sbjct: 458 TWDNPSKFMPERFINNN------------NDNAMEFYPFGYGKKSCAGVALAERMLMFIL 517

Query: 484 ASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLYLA 531
           ASLLHSF+WELPKDSVIDFKEKFGIVNKKLNPLVAIPTP+LSNSDLYLA
Sbjct: 518 ASLLHSFDWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPTLSNSDLYLA 553

BLAST of Cucsa.302230.1 vs. NCBI nr
Match: gi|659073582|ref|XP_008437139.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X1 [Cucumis melo])

HSP 1 Score: 714.9 bits (1844), Expect = 1.0e-202
Identity = 356/528 (67.42%), Postives = 420/528 (79.55%), Query Frame = 1

Query: 1   MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG 60
           M L+S PFLF FWS+DS+  NQIST ILFIFLF+FALLWLRSKFRRPSLPPGPRGLPLVG
Sbjct: 1   MPLVSIPFLFSFWSLDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVG 60

Query: 61  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV 120
           YLPFLS N HH    LAK+YG + KL LG KLC+VLTSP+ + EA  HQET+F  R+TTV
Sbjct: 61  YLPFLSPNLHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTV 120

Query: 121 CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV 180
            ALLSSYG S IVF QD  +WKKLRKIF RKM+SKSNLDASY +RRQEVRKVIKGVFES 
Sbjct: 121 VALLSSYGGSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESA 180

Query: 181 GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV 240
           GT IDIGK+ F+A +KSV+AMTWG SG ++G DG  L+ KFRE +DE++VLL + N+SD+
Sbjct: 181 GTPIDIGKLGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDL 240

Query: 241 FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN 300
           FPVLGRFDLQG+ R+ KKVM V D I NSAIEEQRK GGNG    G+LQ LLE+ + ED+
Sbjct: 241 FPVLGRFDLQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDS 300

Query: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 360
           SE IT + LKALL+DIVIGGT+TTSTT+EWA++EL++ P+TM KV EEL KV+GLN +VE
Sbjct: 301 SESITENNLKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVE 360

Query: 361 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 420
           EFH PKL +L+AV+KETLRLHP + LLVPRT + +TT G Y IPK STIYFN+W IQRDP
Sbjct: 361 EFHLPKLNFLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDP 420

Query: 421 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI 480
            +WDNPL F PERF+  + E          + N +EF PFG GK+SCAGI LA+R+L+ I
Sbjct: 421 KVWDNPLNFEPERFLNESSENL-----YDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLI 480

Query: 481 LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           LASLLH FEW+LP+ S  D +EKFGIV KKLNPLV IPTP L N +LY
Sbjct: 481 LASLLHGFEWKLPEGSKFDMEEKFGIVTKKLNPLVTIPTPRLPNLELY 523

BLAST of Cucsa.302230.1 vs. NCBI nr
Match: gi|659073584|ref|XP_008437141.1| (PREDICTED: 7-ethoxycoumarin O-deethylase-like isoform X2 [Cucumis melo])

HSP 1 Score: 703.7 bits (1815), Expect = 2.4e-199
Identity = 351/520 (67.50%), Postives = 413/520 (79.42%), Query Frame = 1

Query: 9   LFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSGN 68
           L  FWSVDS+  NQIST ILFIFLF+FALLWLRSKFRRPSLPPGPRGLPLVGYLPFLS N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 69  PHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTVCALLSSYG 128
            HH    LAK+YG + KL LG KLC+VLTSP+ + EA  HQET+F  R+TTV ALLSSYG
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 129 CSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESVGTQIDIGK 188
            S IVF QD  +WKKLRKIF RKM+SKSNLDASY +RRQEVRKVIKGVFES GT IDIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 189 VSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDVFPVLGRFD 248
           + F+A +KSV+AMTWG SG ++G DG  L+ KFRE +DE++VLL + N+SD+FPVLGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 249 LQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDNSECITNDQ 308
           LQG+ R+ KKVM V D I NSAIEEQRK GGNG    G+LQ LLE+ + ED+SE IT + 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 309 LKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVEEFHFPKLC 368
           LKALL+DIVIGGT+TTSTT+EWA++EL++ P+TM KV EEL KV+GLN +VEEFH PKL 
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 369 YLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDPTIWDNPLK 428
           +L+AV+KETLRLHP + LLVPRT + +TT G Y IPK STIYFN+W IQRDP +WDNPL 
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 429 FMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFILASLLHSF 488
           F PERF+  + E          + N +EF PFG GK+SCAGI LA+R+L+ ILASLLH F
Sbjct: 423 FEPERFLNESSENL-----YDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGF 482

Query: 489 EWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           EW+LP+ S  D +EKFGIV KKLNPLV IPTP L N +LY
Sbjct: 483 EWKLPEGSKFDMEEKFGIVTKKLNPLVTIPTPRLPNLELY 517

BLAST of Cucsa.302230.1 vs. NCBI nr
Match: gi|778699919|ref|XP_004147610.2| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 699.1 bits (1803), Expect = 5.8e-198
Identity = 348/528 (65.91%), Postives = 416/528 (78.79%), Query Frame = 1

Query: 1   MSLLSFPFLFLFWSVDSDNQNQISTLILFIFLFMFALLWLRSKFRRPSLPPGPRGLPLVG 60
           M  LS PFLF FWS+D +  +QIST +LF FLF+FALLWL+SKF+ P LPPGPRGLPL+G
Sbjct: 1   MPTLSIPFLFSFWSLDFNKHSQISTALLFFFLFIFALLWLQSKFQHPPLPPGPRGLPLLG 60

Query: 61  YLPFLSGNPHHKLTHLAKIYGPVFKLRLGPKLCVVLTSPAFIKEAFHHQETLFPNRNTTV 120
           YLPFLS N HH   +LAK+YGP+FKLRLG KLCVVLTSP+ + E   HQET+F NR+ TV
Sbjct: 61  YLPFLSTNLHHTFNNLAKMYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATV 120

Query: 121 CALLSSYGCSGIVFAQDGEDWKKLRKIFVRKMISKSNLDASYCVRRQEVRKVIKGVFESV 180
            ALL++YG   IVF+Q+   WKKLRKIF RKM+SKSNLDASY +RR+EVRK+IKGV ES 
Sbjct: 121 SALLATYGGVDIVFSQNESIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESA 180

Query: 181 GTQIDIGKVSFLATMKSVVAMTWGDSGRVMGEDGVGLEVKFREMMDELVVLLGSRNVSDV 240
           GT IDI K+S +A +KSV+AMTWG S  ++G DG  LE KF E+++EL+VLLG+ N+SD+
Sbjct: 181 GTPIDISKLSLMAAVKSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDL 240

Query: 241 FPVLGRFDLQGIGRRTKKVMGVLDGILNSAIEEQRKMGGNGGGKGGYLQLLLELQDNEDN 300
           FPVLG  DLQGIGR+ KKVM V DGI NSAIEEQRK GG+G    G+LQLLLE+ + ED+
Sbjct: 241 FPVLGGLDLQGIGRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDS 300

Query: 301 SECITNDQLKALLLDIVIGGTETTSTTIEWAMAELMQHPDTMKKVKEELKKVIGLNAIVE 360
           SE IT+ +LKALL+DI+IGGT+TTSTTIEW +AEL+Q P+ MKKVKEEL KV+GLN +VE
Sbjct: 301 SESITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVE 360

Query: 361 EFHFPKLCYLNAVIKETLRLHPAIFLLVPRTLTSSTTLGGYYIPKDSTIYFNLWGIQRDP 420
           EFH  KL YL+A IKETLRLHP + LLVPRT    TTL GY IPK STIYFN+W IQRDP
Sbjct: 361 EFHLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDP 420

Query: 421 TIWDNPLKFMPERFVKSNEEECAGQFELGDSNNAMEFYPFGYGKRSCAGIALAERMLMFI 480
            IWDNPL FMPERF+  + E          + N +EF PFG GK+ C GI LAER+L+ I
Sbjct: 421 KIWDNPLDFMPERFLNESNENM-----YDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLI 480

Query: 481 LASLLHSFEWELPKDSVIDFKEKFGIVNKKLNPLVAIPTPSLSNSDLY 529
           LASLLH FEW+LP+ S +D +EKFGIV+KKLNPLV IPTP LSN +LY
Sbjct: 481 LASLLHGFEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELY 523