Cucsa.277660.6 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.277660.6 vs. Swiss-Prot
Match: FAS1_ARATH (Chromatin assembly factor 1 subunit FAS1 OS=Arabidopsis thaliana GN=FAS1 PE=1 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 1.6e-196
Identity = 395/829 (47.65%), Postives = 528/829 (63.69%), Query Frame = 1

Query: 14  LDESSKPSSTD---TQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKYYDE 73
           +DE S  +  +   T   P+K+ KRKR    I +L  EE+E++I  +  E+  LF Y+ E
Sbjct: 1   MDEVSTVNENENRKTMIEPKKLNKRKREPTAIENLTSEEKESQISSLNLEMKGLFDYFRE 60

Query: 74  V--KCQKVDLDLG--QCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 133
           V  K ++ DL  G  +CSS +S+VA LMEE  L LSKLVDEIY K+K+       E+VT+
Sbjct: 61  VMDKSKRTDLFSGFSECSSLNSMVALLMEEMSLPLSKLVDEIYLKLKE-----KTESVTM 120

Query: 134 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 193
            +VK++V+ VG+RV YGV N DADVLED S  CLWCWETRDLK+MP S RG+L +RRTCR
Sbjct: 121 VAVKSAVVSVGQRVSYGVLNVDADVLEDDSESCLWCWETRDLKIMPSSVRGVLKLRRTCR 180

Query: 194 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 253
           KKI ER+T +SAM ++L + ET++    + +KA+++L K+  E  IR   D + QK ++E
Sbjct: 181 KKIHERITAVSAMLAALQREETEKLWRSDLSKAAEKLGKILSEVDIRSFMDNMMQKNSSE 240

Query: 254 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTER---EEKRKE 313
           MAEK++KREEKL++KQLE+N+ EAEKEKKR++R+  KEKLQ EKE K+ ++   +E  KE
Sbjct: 241 MAEKDSKREEKLLLKQLEKNRCEAEKEKKRMERQVLKEKLQQEKEQKLLQKAIVDENNKE 300

Query: 314 KEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPND 373
           KEE E +K+++KQQ+++EKEQ+RREKE+AE KKQL +QKQASIMERFLKKSK SS     
Sbjct: 301 KEETESRKRIKKQQDESEKEQKRREKEQAELKKQLQVQKQASIMERFLKKSKDSSLTQPK 360

Query: 374 QSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSR 433
             ++E+        K EN +    Q +D   S++      DIRR+H +SWR +G  + S 
Sbjct: 361 LPSSEVTAQELSCTKHENEIGKVVQAIDNAFSTTCEATVDDIRREHFASWRQLGHLLSS- 420

Query: 434 GEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTL 493
            +KHWG+RR+PKSELF +LKLS      +D E   E+  DG EE   D    +    +  
Sbjct: 421 SKKHWGMRRQPKSELFPKLKLSTNSGVTSDGEPNMEKQGDGCEENNFDGRQCKPSSSNR- 480

Query: 494 LDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEW---- 553
              +KS R KQLLQF KS RP FYGIW S+S VV PR P +KDP+LDY+VDSDEEW    
Sbjct: 481 ---KKSRRVKQLLQFDKSCRPGFYGIWPSQSQVVKPRRPLQKDPELDYEVDSDEEWEEEE 540

Query: 554 ------------------------------------------EEGVQLDRMDTDDVDEVR 613
                                                     +EGVQ+DRMD D  ++  
Sbjct: 541 AGESLSDCEKDEDESLEEGCSKADDEDDSEDDFMVPDGYLSEDEGVQVDRMDIDPSEQDA 600

Query: 614 STPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDSLLMAEDLDCTS 673
           +T SSKQD E  E  ++L+QQKHL N+T+ AL+K QPLII NL HEK SLL A+DL+ T 
Sbjct: 601 NTTSSKQDQESPEFCALLQQQKHLQNLTDHALKKTQPLIICNLTHEKVSLLAAKDLEGTQ 660

Query: 674 KLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEV----CVPSDKDNGTQISTSTILDSE 733
           K+EQ CL AL +   P   LIE+S++ + DED E     C  S   + ++     I DS+
Sbjct: 661 KVEQICLRALMVRQFPWSSLIEISINDIQDEDQEASKFSCSQSTPPSNSK--AKIIPDSD 720

Query: 734 MTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQVCKRILTFGD 783
           +  +VSTIQSCSQGIN+VVE+LQ KFP VPKT LR KVRE+SDF ++RWQV K +LT   
Sbjct: 721 LLTVVSTIQSCSQGINRVVETLQQKFPDVPKTKLRQKVREISDFEDSRWQVKKEVLT--K 780

BLAST of Cucsa.277660.6 vs. Swiss-Prot
Match: FAS1_ORYSJ (Chromatin assembly factor 1 subunit FSM OS=Oryza sativa subsp. japonica GN=FSM PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.0e-101
Identity = 238/647 (36.79%), Postives = 350/647 (54.10%), Query Frame = 1

Query: 176 RGILNIRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLL 235
           RG  N   T ++ +QE V    +   + +    D + +Q+ T  +++             
Sbjct: 289 RGAKNTGSTAKEPMQEMV---KSNNDTGIIENVDDSQLQKNTSTNEKD------------ 348

Query: 236 TDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVT 295
           T    +++  E+ +KE  +EE  M KQ ++ Q EA +E+KR ++E+ + K Q  K+ +  
Sbjct: 349 TQKAQKQVEKELKQKE--KEEARMRKQQKKQQEEALREQKRREKEEAEMKKQQRKQEEEA 408

Query: 296 EREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSK 355
           ++E+KR+EKEE E +KQ +KQQE+AEKEQ+RREKE  + KKQL++QKQAS+MERF K  K
Sbjct: 409 QKEQKRREKEEAETRKQQKKQQEEAEKEQKRREKEAVQLKKQLAIQKQASMMERFFKNKK 468

Query: 356 PSSSFPNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRL 415
            S            + +      ++ V+   T ++D + S  +     D+RR  +S W+ 
Sbjct: 469 DSEKLEKPGGKDSGVQTTDPCTTNKEVVPLVTSIIDSSFSQKENWALEDLRRLQISGWQK 528

Query: 416 IGSSIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELG--EERLVDGWEEQITDAG 475
           + S  RS     WGIR KPK E FKELKL    ++  ++ L   E+   +  +E   D  
Sbjct: 529 LSSYNRS---SRWGIRNKPKKEAFKELKLQKTSDNMLEEILSPNEDTCHNLSQENEPDKS 588

Query: 476 TSQTELCSTL-LDVRKSNRG---------KQLLQFAKSYRPAFYGIWSSKSHVVGPRHPF 535
            +  ++   + L    +N           ++LLQF KS RPA+YG W  KS VVGPR P 
Sbjct: 589 ANDVDMLPAVELQFHGTNHANPLPTRSIKRKLLQFDKSNRPAYYGTWRKKSAVVGPRCPL 648

Query: 536 RKDPDLDYDVDSDEEWEE------------------------------------------ 595
           + DPDLDY+VDSD+EWEE                                          
Sbjct: 649 KMDPDLDYEVDSDDEWEEEDPGESLSDCEKDNDEVMEEDSKITDEESEDSFFVPDGYLSD 708

Query: 596 --GVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILN 655
             G+Q++ +  DD DE  S+P   Q  E +E  ++L+QQK L+ +T  ALRK+QPL+I N
Sbjct: 709 NEGIQIESL-LDDKDEASSSPPD-QCAEVEEFRALLRQQKVLNTLTEQALRKSQPLVISN 768

Query: 656 LLHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSV----DGMADEDPEVCVP 715
           L HEK  LL A DL  TSK+EQ CL  LSM + PGG  I++ V       A+E  ++ V 
Sbjct: 769 LTHEKAELLTAGDLKGTSKIEQLCLQVLSMRICPGGATIDLPVIDSSSANAEETNQLNVK 828

Query: 716 SDKDNGTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVS 763
           S     +  + S I D+++  IV  I SC  GINK+VESL  KFP+V K+ L+NKVRE+S
Sbjct: 829 S-----SPAAASAIPDTDLAEIVKVIGSCRDGINKLVESLHQKFPNVSKSQLKNKVREIS 888

HSP 2 Score: 357.5 bits (916), Expect = 3.9e-97
Identity = 279/860 (32.44%), Postives = 413/860 (48.02%), Query Frame = 1

Query: 20  PSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKYYDEVKCQKVDLD 79
           P+  DT    +K  KRKR      +L   +++A + G  +E++ L +YY EV   ++  +
Sbjct: 71  PALMDTIVEVQKQLKRKRASSG-PALAAADKDALVAGCCQELEGLLEYYREVSGHRMQFE 130

Query: 80  LGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTVASVKASVLFVGRR 139
           +G  S++ +I   L+EES L LSKLVDEIYEK+K +      E V+  SV++SVL +G+R
Sbjct: 131 VGNLSTNAAI-GCLLEESSLGLSKLVDEIYEKLKGM------EGVSATSVRSSVLLIGQR 190

Query: 140 VMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCRKKIQERVTVLSAM 199
           +MYG  + DADVLED S   LWCWE RDLK++P   RG L+ RRT RKKI ER+T + + 
Sbjct: 191 MMYGQSSPDADVLEDESETALWCWEVRDLKVIPLRMRGPLSTRRTARKKIHERITAIYS- 250

Query: 200 KSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKE----AKRE 259
             S+L++   +  + +  KAS +LSK  +   I+ L +  +QK   E   K     AK  
Sbjct: 251 TLSVLEAPGAEAQVNDMRKASLKLSKALNLEGIKSLVERATQKSNIERGAKNTGSTAKEP 310

Query: 260 EKLMVK--------------QLERN-----------QREAEKEKKRIDREQQKEKLQNEK 319
            + MVK              QL++N           Q++ EKE K+ ++E+ + + Q +K
Sbjct: 311 MQEMVKSNNDTGIIENVDDSQLQKNTSTNEKDTQKAQKQVEKELKQKEKEEARMRKQQKK 370

Query: 320 ESKVTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAE----------------- 379
           + +   RE+KR+EKEE EMKKQ RKQ+E+A+KEQ+RREKEEAE                 
Sbjct: 371 QQEEALREQKRREKEEAEMKKQQRKQEEEAQKEQKRREKEEAETRKQQKKQQEEAEKEQK 430

Query: 380 --------FKKQLSLQKQASIMERFLKKSKPSSSFPNDQSTTELIISVPLSKKSENVLQA 439
                    KKQL++QKQAS+MERF K  K S            + +      ++ V+  
Sbjct: 431 RREKEAVQLKKQLAIQKQASMMERFFKNKKDSEKLEKPGGKDSGVQTTDPCTTNKEVVPL 490

Query: 440 CTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGEKHWGIRRKPKSELFKELKLS 499
            T ++D + S  +     D+RR  +S W+ + S  RS     WGIR KPK E FKELKL 
Sbjct: 491 VTSIIDSSFSQKENWALEDLRRLQISGWQKLSSYNRS---SRWGIRNKPKKEAFKELKLQ 550

Query: 500 AGRESANDDEL--GEERLVDGWEEQITDAGTSQTELCSTL-LDVRKSNRG---------K 559
              ++  ++ L   E+   +  +E   D   +  ++   + L    +N           +
Sbjct: 551 KTSDNMLEEILSPNEDTCHNLSQENEPDKSANDVDMLPAVELQFHGTNHANPLPTRSIKR 610

Query: 560 QLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE---GVQLDRMDT 619
           +LLQF KS RPA+YG W  KS VVGPR P + DPDLDY+VDSD+EWEE   G  L   + 
Sbjct: 611 KLLQFDKSNRPAYYGTWRKKSAVVGPRCPLKMDPDLDYEVDSDDEWEEEDPGESLSDCEK 670

Query: 620 DDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDSLLMA 679
           D+ DEV    S   D E ++ + V           +  L  N+ + I +LL +KD    +
Sbjct: 671 DN-DEVMEEDSKITDEESEDSFFV----------PDGYLSDNEGIQIESLLDDKDEASSS 730

Query: 680 EDLDCTSKLEQTCLAALSMCLMPGGCLIEMS--------VDGMADEDPEVCVPSDKDNGT 739
               C    E   L      L     L E +        +  +  E  E+    D    +
Sbjct: 731 PPDQCAEVEEFRALLRQQKVL---NTLTEQALRKSQPLVISNLTHEKAELLTAGDLKGTS 790

Query: 740 -------QISTSTILDSEMTAIVSTIQSCSQGINKV----VESLQLKFPSVPKTHLRNKV 763
                  Q+ +  I     T  +  I S S    +     V+S      ++P T L   V
Sbjct: 791 KIEQLCLQVLSMRICPGGATIDLPVIDSSSANAEETNQLNVKSSPAAASAIPDTDLAEIV 850

BLAST of Cucsa.277660.6 vs. Swiss-Prot
Match: CA1AB_XENLA (Chromatin assembly factor 1 subunit A-B OS=Xenopus laevis GN=chaf1a-b PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 9.8e-24
Identity = 99/349 (28.37%), Postives = 164/349 (46.99%), Query Frame = 1

Query: 218 KASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRI 277
           K S    K  D+A+ R      ++K   E A +EA+       K L + +RE E+E++  
Sbjct: 253 KTSAEKKKTKDKAEKRQ-----AEKEERECARREAR-----AAKDLAKKKREGEREQREK 312

Query: 278 DREQQKEKLQNEKESKVTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQ 337
           D++++KE+   EK  K   +EEK+KEK E    KQ  K++++ EK Q+  EK   E +K+
Sbjct: 313 DKKEKKEREDREKAEKNRLKEEKKKEKLEALEAKQEEKRKKEEEKRQKEEEKRLKEEEKR 372

Query: 338 LSLQKQASIMERFLKKSKPSSSFPNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSS 397
           +  +K  + + RFL+K K   +      +        + KK   +   C   +D    +S
Sbjct: 373 IKAEK--AEITRFLQKPKTPQAPKTFARSCGKFAPFEI-KKGMALAPLCR--IDFEQEAS 432

Query: 398 DAIIPVDIRRQHLSSWRLIGSSIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELG 457
           + +   DI  Q  +S       I+ R  +  G    P     +   +    E+  D  LG
Sbjct: 433 EEL---DIFLQEQTSESSFLDEIKKRRPRKMGQTTVPTINSVEVDDVQVLGET--DPVLG 492

Query: 458 EERLVDGWEEQITDAGTSQTELCSTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVV 517
              ++   EE I D G  +          RK     +LLQF +++RPA++G  + +S V+
Sbjct: 493 SNMVL---EEHIKDIGVPE----------RKKFGRMKLLQFCENHRPAYWGTSNKRSRVI 552

Query: 518 GPRHPFRKDPD-LDYDVDSDEEWEEGVQLDRMDTDDVDEVRSTPSSKQD 566
            PR P+ +D D LDY+VDSDEEWEE    + +   + +     P  ++D
Sbjct: 553 NPRKPWAQDTDMLDYEVDSDEEWEEEEPGESLSHSEGENEDDDPKEEED 568

BLAST of Cucsa.277660.6 vs. Swiss-Prot
Match: CAF1A_MOUSE (Chromatin assembly factor 1 subunit A OS=Mus musculus GN=Chaf1a PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.7e-18
Identity = 92/344 (26.74%), Postives = 164/344 (47.67%), Query Frame = 1

Query: 242 KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDR--EQQKEKLQNEKESKVTEREE 301
           ++A    +   ++    + +  E+ + E EK ++ I R  E+ ++K + EKE K  ER E
Sbjct: 301 RVAKNFVKGSTEKGRSKLHRDREQQREEKEKLREEIRRAKEEARKKKEEEKELKEKERRE 360

Query: 302 KR----KEK-EENEMKKQLRKQQEDA-----------EKEQRRREKEEAEFKKQLSLQKQ 361
           KR    KEK E+  +K++ RK++++A           E+E+R RE+E+   +++  ++ +
Sbjct: 361 KREKDEKEKAEKQRLKEEKRKERQEALEAKLEEKRKKEEEKRLREEEKRLREEEKRIKAE 420

Query: 362 ASIMERFLKKSKPSSSFPNDQSTTELIIS----VPLSKKSENVL-QACTQLMDCTLSSSD 421
            + + RF +K K        Q+   L  S     P   K   VL   C   +D  L    
Sbjct: 421 KAEITRFFQKPK------TPQAPKTLAGSCGKFAPFEIKEHMVLAPRCRAALDQDLCDQ- 480

Query: 422 AIIPVDIRRQHLSSWRLIGSSIRSRGEKHWGIRRKPKSELFKELKLSAG--RESANDDEL 481
             +   +++Q ++S  L                    S+L   L L +G  R   +D ++
Sbjct: 481 --LDQLLQQQSVASTFL--------------------SDLKSRLPLRSGPTRVCGHDTDI 540

Query: 482 GEERLVDGWEEQITDAGTSQTELCSTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHV 541
               +V     ++   G S+          RK     +LLQF++++RPA++G W+ K+ +
Sbjct: 541 MNRDVVIVESSKVD--GVSE----------RKKFGRMKLLQFSENHRPAYWGTWNKKTAI 600

Query: 542 VGPRHPFRKDPD-LDYDVDSDEEWEE---GVQLDRMDTDDVDEV 557
           + PR+P+ +D D LDY+VDSD+EWEE   G  L   + D+ D+V
Sbjct: 601 IRPRNPWAQDKDLLDYEVDSDDEWEEEEPGESLSHSEGDEDDDV 603

HSP 2 Score: 62.0 bits (149), Expect = 3.4e-08
Identity = 39/126 (30.95%), Postives = 67/126 (53.17%), Query Frame = 1

Query: 222 RLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQ 281
           R++K F +         L +    +  EKE  REE    K+  R ++E EKE K  +R +
Sbjct: 301 RVAKNFVKGSTEKGRSKLHRDREQQREEKEKLREEIRRAKEEARKKKEEEKELKEKERRE 360

Query: 282 QKEKLQNEKESKVTEREEKRKEKEE-----------NEMKKQLRKQQEDAEKEQRRREKE 337
           ++EK + EK  K   +EEKRKE++E            E +K+LR++++   +E++R + E
Sbjct: 361 KREKDEKEKAEKQRLKEEKRKERQEALEAKLEEKRKKEEEKRLREEEKRLREEEKRIKAE 420

BLAST of Cucsa.277660.6 vs. Swiss-Prot
Match: CAF1A_CHICK (Chromatin assembly factor 1 subunit A OS=Gallus gallus GN=CHAF1A PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 8.0e-18
Identity = 89/339 (26.25%), Postives = 165/339 (48.67%), Query Frame = 1

Query: 239 LSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKV---- 298
           +SQK+    AEKE  R        L+R+Q  A+K +K     ++K +L+  +E+K     
Sbjct: 294 VSQKLHKSSAEKEKLR--------LQRDQERADKLQKLQAEREEKGRLK--EEAKAAKER 353

Query: 299 TEREEKRKEKEENEMKKQLR---KQQEDAEKEQRRREKEEAEFKKQLSL-----QKQASI 358
            + E K+K++EE E+K++ R   K++E+ EK ++ R KEE   ++Q +L     +K+   
Sbjct: 354 AKEEAKKKKEEEKELKERERREKKEKEEKEKAEKLRVKEEKRKERQEALEAKLEEKRKKE 413

Query: 359 MERFLKKSKPSSSFPNDQSTTELIISVPLSKKSENVLQ-ACTQLMDCTLSSSDAIIPVDI 418
            E+ LK+ +   +    + T       P + ++  +L  +C +     +  +  + P+  
Sbjct: 414 EEKRLKEEEKRINAQKAEITR--FFQKPKTPQAPKILAGSCGKFAPFEIKENMVLAPLCR 473

Query: 419 RRQHLSSWRLIGSSIRSRGEKHWGIR----RKPKSELFKELKLSAGRESANDDELGEERL 478
              +      +   +R++  +   +R    RKP+        +++  ++ N D +    +
Sbjct: 474 IALYPDFLEQLDRLLRAQNSEVSFLRDLKCRKPRKT--GPTFVNSSTDTVNSDVV----V 533

Query: 479 VDGWEEQITDAGTSQTELCSTLLDVRKSNRGK-QLLQFAKSYRPAFYGIWSSKSHVVGPR 538
           VD                C T     +   G+ +LLQF +++RPA++G W+ K+ ++ PR
Sbjct: 534 VDN---------------CKTDAVPERGKFGRMKLLQFCENHRPAYWGTWNKKTTMIRPR 593

Query: 539 HPFRKDPD-LDYDVDSDEEWEE---GVQLDRMDTDDVDE 556
           +P+ KD   LDY+VDSDEEWEE   G  L   + DD +E
Sbjct: 594 NPWSKDSKLLDYEVDSDEEWEEEEPGESLSHSEGDDEEE 599

HSP 2 Score: 51.2 bits (121), Expect = 6.0e-05
Identity = 35/115 (30.43%), Postives = 72/115 (62.61%), Query Frame = 1

Query: 206 SETDQTCIQEFTKASDRLSKVFDEA--KIRLLTDGLSQKIATEMAEKEAKR--EEKLMVK 265
           +E ++  +Q   + +D+L K+  E   K RL  +    K A E A++EAK+  EE+  +K
Sbjct: 303 AEKEKLRLQRDQERADKLQKLQAEREEKGRLKEEA---KAAKERAKEEAKKKKEEEKELK 362

Query: 266 QLERNQREAEKEKKRIDREQQKEKLQNE-KESKVTEREEKRKEKEENEMKKQLRK 316
           + ER +++ ++EK++ ++ + KE+ + E +E+   + EEKRK++EE  +K++ ++
Sbjct: 363 ERERREKKEKEEKEKAEKLRVKEEKRKERQEALEAKLEEKRKKEEEKRLKEEEKR 414

HSP 3 Score: 31.2 bits (69), Expect = 6.4e+01
Identity = 33/133 (24.81%), Postives = 53/133 (39.85%), Query Frame = 1

Query: 232 IRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKE 291
           + L  +G S    + ++       E   V + +RN              Q+  K   EKE
Sbjct: 247 VELSHEGDSVLTNSSLSSLSVSSPEAQPVAETKRNTSPLAVSTPVRKVSQKLHKSSAEKE 306

Query: 292 SKVTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEA---------EFKKQLSLQK 351
               +R+++R +K           Q+  AE+E++ R KEEA         E KK+   +K
Sbjct: 307 KLRLQRDQERADK----------LQKLQAEREEKGRLKEEAKAAKERAKEEAKKKKEEEK 366

Query: 352 QASIMERFLKKSK 356
           +    ER  KK K
Sbjct: 367 ELKERERREKKEK 369

BLAST of Cucsa.277660.6 vs. TrEMBL
Match: A0A0A0LMY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G083770 PE=4 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 3.9e-306
Identity = 546/567 (96.30%), Postives = 550/567 (97.00%), Query Frame = 1

Query: 2   SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI 61
           SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI
Sbjct: 51  SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI 110

Query: 62  DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV 121
           DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV
Sbjct: 111 DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV 170

Query: 122 EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI 181
           EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI
Sbjct: 171 EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI 230

Query: 182 RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ 241
           RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ
Sbjct: 231 RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ 290

Query: 242 KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR 301
           KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR
Sbjct: 291 KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR 350

Query: 302 KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP 361
           KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP
Sbjct: 351 KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP 410

Query: 362 NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR 421
           NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR
Sbjct: 411 NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR 470

Query: 422 SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS 481
           SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS
Sbjct: 471 SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS 530

Query: 482 TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE 541
           TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE
Sbjct: 531 TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE 590

Query: 542 ---GVQLDRMDTDDVDEVRSTPSSKQD 566
              G  L   D DD + +     +K +
Sbjct: 591 EDPGESLSDCDKDDEESLEEEGCAKAE 617

BLAST of Cucsa.277660.6 vs. TrEMBL
Match: A0A0A0LMY1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G083770 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 4.6e-129
Identity = 240/253 (94.86%), Postives = 241/253 (95.26%), Query Frame = 1

Query: 541 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 600
           EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL
Sbjct: 637 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 696

Query: 601 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 660
           LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN
Sbjct: 697 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 756

Query: 661 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 720
           GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN
Sbjct: 757 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 816

Query: 721 RWQVCKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 780
           RWQV K IL   +K       EKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD
Sbjct: 817 RWQVKKAIL---EKHGVLPSPEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 876

Query: 781 SAVQGQRTCTNQQ 794
           SAVQGQRTCTNQQ
Sbjct: 877 SAVQGQRTCTNQQ 886

HSP 2 Score: 809.7 bits (2090), Expect = 3.2e-231
Identity = 447/841 (53.15%), Postives = 573/841 (68.13%), Query Frame = 1

Query: 8   DAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKY 67
           + V +D  ++     ++ Q R RK QKRKR       L  E +EA+IE  ++++D LF +
Sbjct: 4   EVVAIDGGDNPIGRKSNDQDRVRKTQKRKRVSFSPECLGLEAKEAQIESFRKQLDGLFGF 63

Query: 68  YDEVKCQKVDLDLGQCSSS-DSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 127
           Y EV  Q+VDLD+  C ++ +S++ AL+EES L LSKLV+E++EK+K  +       VT+
Sbjct: 64  YMEVMGQRVDLDVKLCGNNMNSVIGALIEESGLPLSKLVEEVFEKVK--NGNEAFGNVTL 123

Query: 128 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 187
           A VK+ VLFVG+RVMYGVPN DADVLED S  CLWCWETRD+KLMP   RG+LNIRRTCR
Sbjct: 124 ACVKSIVLFVGQRVMYGVPNVDADVLEDESESCLWCWETRDVKLMPAPVRGVLNIRRTCR 183

Query: 188 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 247
           +KI ER+T +SAM  +L K E+DQ  I +  KAS++L K   EAKIR L D LS K   +
Sbjct: 184 RKIHERITAVSAMAMALQKPESDQNYIHDLMKASEQLDKALCEAKIRSLMDRLSVKNGAD 243

Query: 248 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESK----VTEREEKRK 307
           MA+KEAKREEKL++KQ+ER++R +EKEKKR++RE+QKE+  +EKE K     +E++EKR+
Sbjct: 244 MAKKEAKREEKLLIKQMERDKRVSEKEKKRLERERQKEEWLSEKELKRLQGESEKDEKRR 303

Query: 308 EKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPN 367
           EKEE+EM+K  RKQQEDAEKEQRRREKEEAE KKQLS++KQASIMERF+K+SK   +  +
Sbjct: 304 EKEESEMRKLQRKQQEDAEKEQRRREKEEAELKKQLSIKKQASIMERFVKRSKTIVACQS 363

Query: 368 DQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRS 427
           DQ  T+  +S  LSK SEN+ +  TQ MD TLSS++ II  DIRR H+SSWR +G SIRS
Sbjct: 364 DQFPTKATVSDLLSKNSENMAEVVTQSMDHTLSSNEEIIAEDIRRLHVSSWRHLGHSIRS 423

Query: 428 RGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCST 487
              +HWGIR+KPK+ELFKELKL+  +     D+L  ERLVD W E ++D  + Q     +
Sbjct: 424 NRNQHWGIRQKPKTELFKELKLTTSKGLVRGDDLSTERLVDRWREHVSDDKSCQANTDFS 483

Query: 488 LLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE- 547
           L DV+K  RGKQLLQF KS RPAFYGIW  KSHVV P HPFRKDPDLDYDVDSDEEWEE 
Sbjct: 484 LTDVKKCKRGKQLLQFDKSCRPAFYGIWPKKSHVVRPCHPFRKDPDLDYDVDSDEEWEEE 543

Query: 548 ----------------------------------------------GVQLDRMDTD-DVD 607
                                                         GVQ+DRM+TD   +
Sbjct: 544 DPGESLSDCDKDDEEEGLEEGCSKADDEDESEDGFFVPDGYLSENEGVQVDRMETDITYE 603

Query: 608 EVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDSLLMAEDLD 667
           E R +PS  QD+E ++   +L+QQK+L N+T  +L+KNQPLII NL+HEK SLL AEDL+
Sbjct: 604 ETRISPSFTQDLESEKFSILLRQQKYLGNLTERSLQKNQPLIISNLMHEKVSLLTAEDLN 663

Query: 668 CTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDNGTQISTSTIL-DSE 727
              KLEQ CL ALSM + PG   +E+SVDG+ +ED EV + +       IS+ T++ +S+
Sbjct: 664 GILKLEQMCLQALSMHIFPGSSPVEISVDGLPEEDQEVFLSNGTPCVKSISSVTVIPESD 723

Query: 728 MTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQVCKRILTFGD 787
           +  IVS IQSCSQGINKV+++LQ KFP + K+ LRNKVRE+SDF +NRWQV K IL   D
Sbjct: 724 LPTIVSAIQSCSQGINKVLQTLQKKFPDMSKSQLRNKVREISDFADNRWQVKKEIL---D 783

Query: 788 KTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQ-SLEPDSAVQGQRTCTNQ 794
           K       EK   + K+IAAFFSKRCLPP GK  NPN  SPQ +++P    QGQ+ C  +
Sbjct: 784 KVGFSISPEKRAGQTKSIAAFFSKRCLPPTGKSFNPNEKSPQPAVKPGCFGQGQQGCAYE 839

BLAST of Cucsa.277660.6 vs. TrEMBL
Match: A5C513_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_008038 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 5.3e-226
Identity = 442/873 (50.63%), Postives = 583/873 (66.78%), Query Frame = 1

Query: 9   AVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKYY 68
           A V+++   ++ S +D    P+K  KRKR      +   E+R+ARI  ++ E++ LF+Y+
Sbjct: 5   AEVVEVLADAEMSGSD---HPKKSLKRKRISPVAGAPTVEDRKARIGALRAEMEGLFRYF 64

Query: 69  DEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTVAS 128
           +EV  +KVDL++GQC S +++VA L+EES L LSKLV EIYEK+K  DNGG    VT+A+
Sbjct: 65  EEVMGEKVDLEVGQCGSMNAVVAVLLEESRLPLSKLVSEIYEKVKVRDNGG---GVTLAT 124

Query: 129 VKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCRKK 188
           VK+S + VG+R+ YGVPNADADVLED +  CLWCWETRD+KLMPKS RG+L IRRTCRKK
Sbjct: 125 VKSSAVLVGQRLAYGVPNADADVLEDETASCLWCWETRDIKLMPKSVRGLLKIRRTCRKK 184

Query: 189 IQERVT-----------VLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTD 248
           + ER++           ++SAM ++L K E+DQ    +  KAS++L+KV +EA IRLL +
Sbjct: 185 VHERISAVSAASYQHLHLISAMINALEKPESDQNYKYDLIKASEKLAKVLNEADIRLLIE 244

Query: 249 GLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESK---- 308
            + QK   +MAEK+ KREEK+++KQLE+ +RE EKEKKRI+RE QKEKLQNE+E K    
Sbjct: 245 SMVQKDGADMAEKDVKREEKILIKQLEKKKREDEKEKKRIERELQKEKLQNERELKRLQD 304

Query: 309 VTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKK 368
             E++E+R+EKEE+E++KQLRKQQE+AEK+QRRREKEEAE KKQL++QKQASIMERF+K+
Sbjct: 305 EAEKDERRREKEESEIRKQLRKQQEEAEKDQRRREKEEAELKKQLAIQKQASIMERFVKR 364

Query: 369 SKPSSSFPNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSW 428
           +K +S+  NDQS+T+   S   + KSE + ++ T  MD  LSS D I   +IR+ HL+SW
Sbjct: 365 NKNNSTSLNDQSSTKATTSDSSTNKSEKMPESVTLSMDFVLSSKDGIDSEEIRKSHLASW 424

Query: 429 RLIGSSIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAG 488
           R    S RS  ++HWGIRRKPK+EL KE+KL+  R  A D+EL  E++VDGWEE   +  
Sbjct: 425 RY---SDRSNRKQHWGIRRKPKTELVKEIKLTGNRGLARDNELSIEKIVDGWEETTAEDR 484

Query: 489 TSQTELCSTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDV 548
              T   S   D ++    KQLLQF KS+RPAFYGIW  KS +VGPR PF+KD DLDYD+
Sbjct: 485 LFDTNAYSCPSDAQEVQSNKQLLQFDKSHRPAFYGIWPKKSQIVGPRCPFKKDXDLDYDI 544

Query: 549 DSDEEWEE----------------------------------------------GVQLDR 608
           DSDEEWEE                                              GVQ+D+
Sbjct: 545 DSDEEWEEEDPGESLSDCDKDDEEESVEEGCLKGDDDESEDDFMVPDGYLSENEGVQVDK 604

Query: 609 MDTDD-VDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDS 668
           M+TD  V+E RS+P  + + E +E   +L+QQKHLHN+T  ALRKNQPLIILNL+HEK  
Sbjct: 605 METDPTVEEARSSPGCRTEFESEEFCVLLRQQKHLHNLTERALRKNQPLIILNLMHEKIP 664

Query: 669 LLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSV-DGMADEDPEVCVPSDKDNGTQIS 728
           LLMAEDL  T KLEQ CL ALSMC  PGG LIE+SV + + DED E C+ + + + T +S
Sbjct: 665 LLMAEDLSGTPKLEQMCLQALSMCAFPGGPLIEISVTNDLQDEDKEACLSNSRSSTTPVS 724

Query: 729 TS-TILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQ- 788
           T   I+DS++  IV+TIQ+C+QGINK+VESLQLKFP++PK+ LRNKVRE+SDFV+NRWQ 
Sbjct: 725 TGMAIVDSDLPKIVATIQACTQGINKLVESLQLKFPAIPKSQLRNKVREISDFVDNRWQG 784

Query: 789 -------------------VCKRILTFG--DKTITHTL-----LEKGTRRPKTIAAFFSK 790
                              + +  +      K + H L      EKG  R K+IAAFFSK
Sbjct: 785 QDPPRHSYQAVISIELYAPLSRLFMAMSKVKKDVLHKLGLSISPEKG-GRTKSIAAFFSK 844

BLAST of Cucsa.277660.6 vs. TrEMBL
Match: B9HHZ2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0008s07740g PE=4 SV=2)

HSP 1 Score: 784.3 bits (2024), Expect = 1.4e-223
Identity = 437/846 (51.65%), Postives = 566/846 (66.90%), Query Frame = 1

Query: 10  VVMDLDESSKPSSTDTQARPRKVQKRKRGCM------EIVSLEKEEREARIEGIQREIDS 69
           + +D ++ SKPS  D   +P+K  KRKR  +      ++V+L  E++E +IE ++RE++ 
Sbjct: 7   MAIDCEDESKPSGQD---QPKKTLKRKRATLTPTQQQQLVNLTGEQKEVQIEELKREMEG 66

Query: 70  LFKYYDEVKCQK------VDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDN 129
           LF YY E   QK      VDL   +C + + +V  LMEES++S SKLV+EIY K+ K   
Sbjct: 67  LFGYYKETMNQKMGFGFGVDLGGSECINVNGMVGLLMEESDMSFSKLVEEIYGKLVKKSG 126

Query: 130 GGVVEAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRG 189
                 +TVA VK++VLFVG+R+ YGVPN DADVLED ++ CLWCWETRDLKLMPKS RG
Sbjct: 127 N-----LTVAVVKSAVLFVGQRITYGVPNVDADVLEDETQSCLWCWETRDLKLMPKSVRG 186

Query: 190 ILNIRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTD 249
            L IRR CR KI ER+T + AM ++L KSETD+    +  K+S +L KV  EA IRLL D
Sbjct: 187 ALKIRRMCRAKIHERITAVFAMITALQKSETDENYKSDLIKSSGKLGKVLREADIRLLVD 246

Query: 250 GLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESK---- 309
           G+ QK   +MAEK+ KREEKL++KQLE+N+RE EKEKKR+D E QKEK Q EKE K    
Sbjct: 247 GMLQKNGADMAEKQVKREEKLIIKQLEKNKREEEKEKKRMDLEFQKEKRQTEKEQKRLQE 306

Query: 310 VTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKK 369
             E++E+R+E+EE EMK+QL++QQE+AEKEQRR+EKEEAE K+++++QKQAS+MERFLK+
Sbjct: 307 EAEKDERRREREEFEMKRQLKRQQEEAEKEQRRKEKEEAELKRRVAVQKQASMMERFLKR 366

Query: 370 SKPSSSFPNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSW 429
           SK SS   NDQS T+   S   SKKS+ + +A TQLMDC    +D I   DI + HLSSW
Sbjct: 367 SKSSSPCQNDQSLTKATTSDSSSKKSKRMDEAVTQLMDCAPLLNDNITSDDILKSHLSSW 426

Query: 430 RLIGSSIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAG 489
             +G SIRS  ++HW IRRKPK+ LFKELKL+A R+  +DD+   E+L  GW +Q +D  
Sbjct: 427 CHLGCSIRSNRKQHWSIRRKPKTGLFKELKLTAIRDPTHDDDSSAEKLDSGWGDQTSDD- 486

Query: 490 TSQTELCSTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDV 549
                   + +DVRK NR KQLLQF KS+RPAFYGIW   SH VGPRHP R+DPDLDYDV
Sbjct: 487 -------ISCIDVRKCNRRKQLLQFDKSHRPAFYGIWPKTSHAVGPRHPLRRDPDLDYDV 546

Query: 550 DSDEEWEE-----------------------------------------------GVQLD 609
           DSDEEWEE                                               GVQ  
Sbjct: 547 DSDEEWEEEDPGESLSDCDKDDGEESLEEEYSKADDEEESEDGFFVPDGYLSENEGVQPH 606

Query: 610 RMDTD-DVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKD 669
           RMD D  V+E RS+PS KQD+E +E  ++LKQQK L+++T+ ALRKN P+I+LN++HEKD
Sbjct: 607 RMDADPSVEEARSSPSCKQDLESEEFCTLLKQQKCLNSLTDNALRKNHPMIVLNIMHEKD 666

Query: 670 SLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDNGTQI- 729
           +LL+A+DL   SK+E+ CL ALSM   PGG  +EM +D ++ E+ + C+ + K + T+I 
Sbjct: 667 ALLVADDLSDISKVEKMCLQALSMRAFPGGPQMEMFLD-VSSENHDACLLNAKASATRIP 726

Query: 730 STSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQV 789
           +  T+ DS+M  +VS IQSCSQ +NKVVESLQ KFP+V K  LRNKVRE+SDFV+NRWQV
Sbjct: 727 AVITLQDSDMPIVVSVIQSCSQSMNKVVESLQQKFPTVSKLQLRNKVREISDFVDNRWQV 786

Query: 790 CKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPDSAVQ 791
            K +L  G   I+    EK   R   I+ FFSKRCLPPAGK  NPN SSP  L+  S  +
Sbjct: 787 KKEVLD-GFGIISSP--EKSRGRKHNISTFFSKRCLPPAGKSTNPNESSPPMLKHGSVAE 832

BLAST of Cucsa.277660.6 vs. TrEMBL
Match: A0A061EUV8_THECC (Chromatin assembly factor 1 subunit A, putative OS=Theobroma cacao GN=TCM_023366 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 7.4e-220
Identity = 443/841 (52.68%), Postives = 568/841 (67.54%), Query Frame = 1

Query: 9   AVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKYY 68
           A V+D+D+  K   TD+Q +P++  KRKR      +L  E+REA+I+ + +E+D L+ YY
Sbjct: 5   ATVIDVDDHPKVPKTDSQDQPKRTGKRKRASWVSETLSDEQREAQIKELYQEMDGLYGYY 64

Query: 69  DEVKCQKVDLDLG------QCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVE 128
            EV  QK    +G      +    +S+VA LMEES+L LS+LV+ I+EK+K  D+ G   
Sbjct: 65  KEVMEQKSGFGMGFGLGLVESGPLNSVVAVLMEESDLPLSRLVEAIHEKVK--DSMG--- 124

Query: 129 AVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIR 188
            V++A+VK++VLFVG+RV YG+ + DAD+LED +   LWCWETRD+KLMPKS R  L IR
Sbjct: 125 NVSLAAVKSAVLFVGQRVKYGLGSEDADILEDDANSSLWCWETRDVKLMPKSVRATLKIR 184

Query: 189 RTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQK 248
           RTCRKKI ER T +SAM + L K E DQ    +F KAS++L KV  EA+IRLL   + QK
Sbjct: 185 RTCRKKINERFTAVSAMITLLQKWENDQNYKHDFMKASEKLLKVLSEAEIRLLMSNMLQK 244

Query: 249 IATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESK----VTERE 308
              EMAEKEAKREEKL++KQ ERN+RE EKEKK++DRE QKEKLQNEKE K      E++
Sbjct: 245 SGAEMAEKEAKREEKLLIKQFERNRREIEKEKKKVDRELQKEKLQNEKERKRLQEEVEKD 304

Query: 309 EKRKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSS 368
           E+R+E+EE EM+KQLRKQQE+ E++QRRREKEEAE KKQLS+QKQAS+MERFLKK K S 
Sbjct: 305 ERRREREEAEMRKQLRKQQEEVERDQRRREKEEAELKKQLSIQKQASLMERFLKKCKTSP 364

Query: 369 SFPNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGS 428
                +  T+     P ++KSE V +A T LMD TLSS       D+R+ HLSSWR +G 
Sbjct: 365 R--QIEQLTKPATFCPSTQKSEKVPEAVTLLMDTTLSSKGETYMDDLRKLHLSSWRHLGH 424

Query: 429 SIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTE 488
            +RS  ++ WG+RRKPK+ELFKELKL+A + S++D EL  ER++DGW E+ +D  +    
Sbjct: 425 FLRSNQKQCWGMRRKPKTELFKELKLTANKGSSHD-ELSVERIIDGWGEENSDDRSCFNP 484

Query: 489 LCSTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEE 548
             S   DV+   R KQLLQF KSYRPAF+GIW  KS+VVGPR P RKDPDLDYDVDSDEE
Sbjct: 485 DISAA-DVKCCGR-KQLLQFDKSYRPAFFGIWPKKSNVVGPRCPLRKDPDLDYDVDSDEE 544

Query: 549 WEE----------------------------------------------GVQLDRMDTD- 608
           WEE                                              GVQ+D   TD 
Sbjct: 545 WEEEEPGESLSDCDKDEEEESFEGCSKADDEDESEDGFFVPDGYLSENEGVQVDGTGTDV 604

Query: 609 DVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDSLLMAE 668
            ++E +S+P S+QD + +E Y+ L+QQK+L+++T  AL+KNQPLIILN+ HEK S+LMAE
Sbjct: 605 ALEETKSSPMSEQDGQNEEFYTFLRQQKYLNSLTEHALQKNQPLIILNISHEKTSVLMAE 664

Query: 669 DLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDNGTQIST-STIL 728
           DL  T KLE TCL ALSM   P G  +E+SVD +AD++ E C+ S K + T + T + IL
Sbjct: 665 DLTNTCKLELTCLQALSMRACPDGSPVEISVDSIADDNQEACLSSSKASTTPVLTVAPIL 724

Query: 729 DSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQVCKRILT 788
           DS+M  IVSTIQSCS GIN++VESLQ KFPS+PK+ L+ KVRE+S+F +NRWQV K IL 
Sbjct: 725 DSDMPLIVSTIQSCSLGINRLVESLQQKFPSIPKSQLKTKVREISEFSDNRWQVKKEIL- 784

Query: 789 FGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQS-LEPDSAVQGQRTC 791
              K       EKG  R KTIAAFFSKRCLPP+ K I+P  +SPQ  L+P SA Q Q++ 
Sbjct: 785 --QKLGIPISPEKGGGRTKTIAAFFSKRCLPPSDKSISPIDTSPQQLLKPSSAAQEQQSY 832

BLAST of Cucsa.277660.6 vs. TAIR10
Match: AT1G65470.1 (AT1G65470.1 chromatin assembly factor-1 (FASCIATA1) (FAS1))

HSP 1 Score: 688.7 bits (1776), Expect = 4.2e-198
Identity = 396/829 (47.77%), Postives = 528/829 (63.69%), Query Frame = 1

Query: 14  LDESSKPSSTD---TQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFKYYDE 73
           +DE S  +  +   T   P+K+ KRKR    I +L  EE+E++I  +  E+  LF Y+ E
Sbjct: 1   MDEVSTVNENENRKTMIEPKKLNKRKREPTAIENLTSEEKESQISSLNLEMKGLFDYFRE 60

Query: 74  V--KCQKVDLDLG--QCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 133
           V  K ++ DL  G  +CSS +S+VA LMEE  L LSKLVDEIY K+K+       E+VT+
Sbjct: 61  VMDKSKRTDLFSGFSECSSLNSMVALLMEEMSLPLSKLVDEIYLKLKE-----KTESVTM 120

Query: 134 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 193
            +VK++V+ VG+RV YGV N DADVLED S  CLWCWETRDLK+MP S RG+L +RRTCR
Sbjct: 121 VAVKSAVVSVGQRVSYGVLNVDADVLEDDSESCLWCWETRDLKIMPSSVRGVLKLRRTCR 180

Query: 194 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 253
           KKI ER+T +SAM ++L + ET++    + +KA+++L K+  E  IR   D + QK ++E
Sbjct: 181 KKIHERITAVSAMLAALQREETEKLWRSDLSKAAEKLGKILSEVDIRSFMDNMMQKNSSE 240

Query: 254 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTER---EEKRKE 313
           MAEK++KREEKL++KQLE+N+ EAEKEKKR++R+  KEKLQ EKE K+ ++   +E  KE
Sbjct: 241 MAEKDSKREEKLLLKQLEKNRCEAEKEKKRMERQVLKEKLQQEKEQKLLQKAIVDENNKE 300

Query: 314 KEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPND 373
           KEE E +K+++KQQ+++EKEQ+RREKE+AE KKQL +QKQASIMERFLKKSK SS     
Sbjct: 301 KEETESRKRIKKQQDESEKEQKRREKEQAELKKQLQVQKQASIMERFLKKSKDSSLTQPK 360

Query: 374 QSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSR 433
             ++E+        K EN +    Q +D   S++      DIRR+H +SWR +G  + S 
Sbjct: 361 LPSSEVTAQELSCTKHENEIGKVVQAIDNAFSTTCEATVDDIRREHFASWRQLGHLLSS- 420

Query: 434 GEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTL 493
            +KHWG+RR+PKSELF +LKLS      +D E   E+  DG EE   D    +    +  
Sbjct: 421 SKKHWGMRRQPKSELFPKLKLSTNSGVTSDGEPNMEKQGDGCEENNFDGRQCKPSSSNR- 480

Query: 494 LDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE-- 553
              +KS R KQLLQF KS RP FYGIW S+S VV PR P +KDP+LDY+VDSDEEWEE  
Sbjct: 481 ---KKSRRVKQLLQFDKSCRPGFYGIWPSQSQVVKPRRPLQKDPELDYEVDSDEEWEEEE 540

Query: 554 --------------------------------------------GVQLDRMDTDDVDEVR 613
                                                       GVQ+DRMD D  ++  
Sbjct: 541 AGESLSDCEKDEDESLEEGCSKADDEDDSEDDFMVPDGYLSEDEGVQVDRMDIDPSEQDA 600

Query: 614 STPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNLLHEKDSLLMAEDLDCTS 673
           +T SSKQD E  E  ++L+QQKHL N+T+ AL+K QPLII NL HEK SLL A+DL+ T 
Sbjct: 601 NTTSSKQDQESPEFCALLQQQKHLQNLTDHALKKTQPLIICNLTHEKVSLLAAKDLEGTQ 660

Query: 674 KLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEV----CVPSDKDNGTQISTSTILDSE 733
           K+EQ CL AL +   P   LIE+S++ + DED E     C  S   + ++     I DS+
Sbjct: 661 KVEQICLRALMVRQFPWSSLIEISINDIQDEDQEASKFSCSQSTPPSNSK--AKIIPDSD 720

Query: 734 MTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVENRWQVCKRILTFGD 783
           +  +VSTIQSCSQGIN+VVE+LQ KFP VPKT LR KVRE+SDF ++RWQV K +LT   
Sbjct: 721 LLTVVSTIQSCSQGINRVVETLQQKFPDVPKTKLRQKVREISDFEDSRWQVKKEVLT--K 780

BLAST of Cucsa.277660.6 vs. TAIR10
Match: AT2G18540.1 (AT2G18540.1 RmlC-like cupins superfamily protein)

HSP 1 Score: 66.6 bits (161), Expect = 7.7e-11
Identity = 41/138 (29.71%), Postives = 77/138 (55.80%), Query Frame = 1

Query: 203 LLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQ 262
           LLK++ +    +  + A   LSK+  E         + ++   E  E E +R+E+   ++
Sbjct: 405 LLKAQKESVIFECASCAEGELSKLMRE---------IEERKRREEEEIERRRKEEEEARK 464

Query: 263 LERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKKQLRKQQEDAEK 322
            E  +R  E+E KR + E+ + K + E+E++  E E KR+E+E    +++ +K++E+AE+
Sbjct: 465 REEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRREEERKKREEEAEQ 524

Query: 323 EQRR---REKEEAEFKKQ 338
            ++R   REKEE   KK+
Sbjct: 525 ARKREEEREKEEEMAKKR 533

HSP 2 Score: 63.2 bits (152), Expect = 8.6e-10
Identity = 31/104 (29.81%), Postives = 66/104 (63.46%), Query Frame = 1

Query: 246 EMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKE 305
           EMA+K  +  ++   +++ER +RE ++ K+R +  +++E+ +  +E     RE++R+ KE
Sbjct: 528 EMAKKREEERQRKEREEVERKRREEQERKRREEEARKREEERKREEEMAKRREQERQRKE 587

Query: 306 ENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMER 350
             E+++++R++QE      R+RE+E A+ ++Q   +K+   MER
Sbjct: 588 REEVERKIREEQE------RKREEEMAKRREQERQKKEREEMER 625

HSP 3 Score: 63.2 bits (152), Expect = 8.6e-10
Identity = 45/163 (27.61%), Postives = 89/163 (54.60%), Query Frame = 1

Query: 186 RKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIAT 245
           ++K +E V      +    + E +    +E  K  + ++K  ++ + R   + + +KI  
Sbjct: 538 QRKEREEVERKRREEQERKRREEEARKREEERKREEEMAKRREQERQRKEREEVERKIRE 597

Query: 246 EMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKE 305
           E   +E KREE++  ++ +  Q++  +E +R  RE++  K + E+ +K+ E E +RKE+E
Sbjct: 598 E---QERKREEEMAKRREQERQKKEREEMERKKREEEARK-REEEMAKIREEERQRKERE 657

Query: 306 ENEMKK---------QLRKQQEDAEK--EQRRREKEEAEFKKQ 338
           + E K+         + RK++E+A K  E+ RR+KEE E K++
Sbjct: 658 DVERKRREEEAMRREEERKREEEAAKRAEEERRKKEEEEEKRR 696

HSP 4 Score: 62.8 bits (151), Expect = 1.1e-09
Identity = 59/210 (28.10%), Postives = 108/210 (51.43%), Query Frame = 1

Query: 153 EDVSRECLWCWETRDLKLMPKSTRGILNIRRTCRKKIQER-VTVLSAMKSSLLKSETDQT 212
           E V  EC  C E    KLM    R I   +R   ++I+ R      A K    K   ++ 
Sbjct: 411 ESVIFECASCAEGELSKLM----REIEERKRREEEEIERRRKEEEEARKREEAKRREEEE 470

Query: 213 CIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAE 272
             +   + ++R  +  +EA+ R        +   +  E+EAKR E+      ER +RE E
Sbjct: 471 AKRREEEETERKKREEEEARKR--------EEERKREEEEAKRREE------ERKKREEE 530

Query: 273 KEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKK----QLRKQQEDAEK--EQR 332
            E+ R   ++++E+ + E+ +K  E E +RKE+EE E K+    + ++++E+A K  E+R
Sbjct: 531 AEQAR---KREEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEEARKREEER 590

Query: 333 RREKEEAEFKKQLSLQKQASIMERFLKKSK 356
           +RE+E A+ ++Q   +K+   +ER +++ +
Sbjct: 591 KREEEMAKRREQERQRKEREEVERKIREEQ 599

BLAST of Cucsa.277660.6 vs. TAIR10
Match: AT5G67320.1 (AT5G67320.1 WD-40 repeat family protein)

HSP 1 Score: 62.8 bits (151), Expect = 1.1e-09
Identity = 92/387 (23.77%), Postives = 165/387 (42.64%), Query Frame = 1

Query: 199 MKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKL 258
           M+++L  SE D      F +  D +SK      ++ L D L +K   E   ++ +   K 
Sbjct: 63  MEANLSNSEVDIDEDFSFFQPLDLISK-----DVKELQDMLREKKRKERDMEKERDRSKE 122

Query: 259 MVKQLERNQ-------REAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKK 318
             K +ER         +E ++ +K+ +RE+++EKL+ EKE +  E+ E+ KE+E  +M++
Sbjct: 123 NDKGVEREHEGDRNRAKEKDRHEKQKEREREREKLEREKE-REREKIEREKEREREKMER 182

Query: 319 QLRKQQED---AEKEQR-RREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQSTT 378
           ++ ++++D    EKE+   RE+E  + +++ S +KQ    +R +   +       D ST 
Sbjct: 183 EIFEREKDRLKLEKEREIEREREREKIEREKSHEKQLGDADREMVIDQTDKEIAGDGSTG 242

Query: 379 EL---IISVPLSKKSE------NVLQACT-QLMDCTLSSSDAIIPVDIRRQHLSSWRLIG 438
                I+  P S+ S        +L+  T ++  C  S S +++           W +  
Sbjct: 243 AEPMDIVMTPTSQTSHIPNSDVRILEGHTSEVCACAWSPSASLLASGSGDATARIWSIPE 302

Query: 439 SSIRSRGEKHWGIRRKPKSELFKELKLSAGRESANDDEL---GEERLV-DGWEEQITDAG 498
            S ++    H G  R   + + K  K  +  +S +   L   GE  L+  G  +      
Sbjct: 303 GSFKA---VHTG--RNINALILKHAKGKSNEKSKDVTTLDWNGEGTLLATGSCDGQARIW 362

Query: 499 TSQTELCSTL-------LDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKD 554
           T   EL STL         ++ + +G  LL  +   R A   +W  K+     +  F   
Sbjct: 363 TLNGELISTLSKHKGPIFSLKWNKKGDYLLTGSVD-RTAV--VWDVKAEEWKQQFEFHSG 422

BLAST of Cucsa.277660.6 vs. TAIR10
Match: AT3G28770.1 (AT3G28770.1 Protein of unknown function (DUF1216))

HSP 1 Score: 62.0 bits (149), Expect = 1.9e-09
Identity = 57/250 (22.80%), Postives = 121/250 (48.40%), Query Frame = 1

Query: 164  ETRDLKLMPKSTRGILNIRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRL 223
            E+RDLK   K        + +   K +++        +  +K E D+   ++  ++  R 
Sbjct: 1054 ESRDLKAKKKEEE-TKEKKESENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSR- 1113

Query: 224  SKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKEK---KRIDRE 283
             K  D+  +  L D  S K   +  EK+  +  KL+ K+ ++ +++  +EK   K I+  
Sbjct: 1114 KKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKKESDKKEKKENEEKSETKEIESS 1173

Query: 284  QQKEKLQNEKESKVTEREEKRKEKEENEM-KKQLRKQQEDAEKE----------QRRREK 343
            + ++   ++KE K ++ ++K+KEKE  E  +K+L+K +ED +K+          + ++EK
Sbjct: 1174 KSQKNEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKKQTSVEENKKQKETKKEK 1233

Query: 344  EEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQSTTELIISVPLSKKSENVLQACTQL 400
             + +  K+ + ++     E    +SK + +    Q+TT+         K+E ++QA +Q 
Sbjct: 1234 NKPKDDKKNTTKQSGGKKESMESESKEAENQQKSQATTQADSD---ESKNEILMQADSQA 1293

HSP 2 Score: 57.4 bits (137), Expect = 4.7e-08
Identity = 45/184 (24.46%), Postives = 88/184 (47.83%), Query Frame = 1

Query: 201  SSLLKSETDQT--CIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAK-REEK 260
            S++ K E D+      E  K  D   +       +L  +    K   E  +  +K RE+K
Sbjct: 954  SNMKKKEEDKKEYVNNELKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKK 1013

Query: 261  LMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKKQLRKQQ 320
               ++  + + EA+KEKK+   ++++EK   E++SK  E+EE R  K + + ++   K++
Sbjct: 1014 EYEEKKSKTKEEAKKEKKKSQDKKREEKDSEERKSK-KEKEESRDLKAKKKEEETKEKKE 1073

Query: 321  EDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQSTTELIISVPLSK 380
             +  K +++ +K+E E  K  S++K+    E+   +   S     D+   E +     +K
Sbjct: 1074 SENHKSKKKEDKKEHEDNK--SMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNK 1133

Query: 381  KSEN 382
            K E+
Sbjct: 1134 KKED 1134

HSP 3 Score: 52.0 bits (123), Expect = 2.0e-06
Identity = 36/142 (25.35%), Postives = 69/142 (48.59%), Query Frame = 1

Query: 248  AEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEEN 307
            ++++ K ++K   +    N ++ E++KK     + K++  N+KE   T + E  K KEEN
Sbjct: 937  SKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELKKQEDNKKE---TTKSENSKLKEEN 996

Query: 308  EMKKQLRKQQEDAEKEQRRRE--------KEEAEFKKQLSLQKQASIMERFLKKSKPSSS 367
            +  K+ ++ ++ A K + ++E        KEEA+ +K+ S  K+    +   +KSK    
Sbjct: 997  KDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKKSQDKKREEKDSEERKSKKEKE 1056

Query: 368  FPNDQSTTELIISVPLSKKSEN 382
               D    +        K+SEN
Sbjct: 1057 ESRDLKAKKKEEETKEKKESEN 1075

HSP 4 Score: 50.4 bits (119), Expect = 5.7e-06
Identity = 41/179 (22.91%), Postives = 84/179 (46.93%), Query Frame = 1

Query: 205  KSETDQTCIQEFTKASD--RLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQ 264
            KS+T +   +E  K+ D  R  K  +E K +   +      A +  E+  +++E    K 
Sbjct: 1019 KSKTKEEAKKEKKKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEKKESENHKS 1078

Query: 265  LERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKKQLRKQQEDAE- 324
             ++  ++  ++ K + +E+ K++ +  +ESK  ++EE +K+ E+ E +   +K+++  E 
Sbjct: 1079 KKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEK 1138

Query: 325  ----------KEQRRREKEEAEFKKQL-SLQKQASIMERFLKKSKPSSSFPNDQSTTEL 370
                      KE  ++EK+E E K +   ++   S      KK K SS     +   E+
Sbjct: 1139 KKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKEM 1197

HSP 5 Score: 44.3 bits (103), Expect = 4.1e-04
Identity = 27/96 (28.12%), Postives = 54/96 (56.25%), Query Frame = 1

Query: 256  EKLMVKQLERNQREAEKEKKRID---REQQKEKLQNEKESK---VTEREEKRKEKEENEM 315
            E +  K+ E+ +   E+ K  I+   +++ K+K + +KESK   + ++EE +KE   NE+
Sbjct: 912  ESVKYKKDEKKEGNKEENKDTINTSSKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNEL 971

Query: 316  KKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQAS 346
            KKQ   ++E  + E  + ++E  + K++   +  AS
Sbjct: 972  KKQEDNKKETTKSENSKLKEENKDNKEKKESEDSAS 1007

BLAST of Cucsa.277660.6 vs. TAIR10
Match: AT1G20920.1 (AT1G20920.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 4.3e-09
Identity = 40/154 (25.97%), Postives = 85/154 (55.19%), Query Frame = 1

Query: 214 QEFTKASDRLSKVFDEAKIRLLTDGLSQKIATEMAEKEAKREEKLMVKQLERNQREAEKE 273
           +E  K  +R  +  D+ +++  ++      + +  E+E    +K  V + ER  RE E++
Sbjct: 71  REKRKEKERERRRRDKDRVKRRSERRKSSDSEDDVEEE-DERDKRRVNEKERGHREHERD 130

Query: 274 -----KKRIDREQQKEKLQNEKESKVTEREEKRKEKEENEMKKQLRKQQEDAEKEQRRRE 333
                K+  +RE++K+K + E+E     RE +R+E+E+  +K++ R+++ED E+++R RE
Sbjct: 131 RGKDRKRDREREERKDK-EREREKDRERREREREEREKERVKERERREREDGERDRRERE 190

Query: 334 KE-------EAEFKKQLSLQKQASIMERFLKKSK 356
           KE       E E  +++  ++    ++R LK+ +
Sbjct: 191 KERGSRRNRERERSREVGNEESDDDVKRDLKRRR 222

HSP 2 Score: 52.0 bits (123), Expect = 2.0e-06
Identity = 30/110 (27.27%), Postives = 56/110 (50.91%), Query Frame = 1

Query: 246 EMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKE 305
           E  E+E +  EK  VK+ ER +RE   + +R  RE++KE+       +   RE   +E +
Sbjct: 155 ERREREREEREKERVKERERRERE---DGERDRREREKERGSRRNRERERSREVGNEESD 214

Query: 306 ENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSK 356
           ++  +   R+++E  E++++ REK      +     K+ S+ +   KK K
Sbjct: 215 DDVKRDLKRRRKEGGERKEKEREKSVGRSSRHEDSPKRKSVEDNGEKKEK 261

BLAST of Cucsa.277660.6 vs. NCBI nr
Match: gi|778668042|ref|XP_011649028.1| (PREDICTED: chromatin assembly factor 1 subunit FAS1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1060.4 bits (2741), Expect = 1.5e-306
Identity = 547/568 (96.30%), Postives = 551/568 (97.01%), Query Frame = 1

Query: 1   MSSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQRE 60
           MSSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQRE
Sbjct: 1   MSSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQRE 60

Query: 61  IDSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGV 120
           IDSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGV
Sbjct: 61  IDSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGV 120

Query: 121 VEAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILN 180
           VEAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILN
Sbjct: 121 VEAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILN 180

Query: 181 IRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLS 240
           IRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLS
Sbjct: 181 IRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLS 240

Query: 241 QKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEK 300
           QKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEK
Sbjct: 241 QKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEK 300

Query: 301 RKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSF 360
           RKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSF
Sbjct: 301 RKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSF 360

Query: 361 PNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI 420
           PNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI
Sbjct: 361 PNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI 420

Query: 421 RSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC 480
           RSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC
Sbjct: 421 RSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC 480

Query: 481 STLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE 540
           STLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE
Sbjct: 481 STLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE 540

Query: 541 E---GVQLDRMDTDDVDEVRSTPSSKQD 566
           E   G  L   D DD + +     +K +
Sbjct: 541 EEDPGESLSDCDKDDEESLEEEGCAKAE 568

BLAST of Cucsa.277660.6 vs. NCBI nr
Match: gi|778668042|ref|XP_011649028.1| (PREDICTED: chromatin assembly factor 1 subunit FAS1 isoform X1 [Cucumis sativus])

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/253 (94.86%), Postives = 241/253 (95.26%), Query Frame = 1

Query: 541 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 600
           EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL
Sbjct: 588 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 647

Query: 601 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 660
           LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN
Sbjct: 648 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 707

Query: 661 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 720
           GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN
Sbjct: 708 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 767

Query: 721 RWQVCKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 780
           RWQV K IL   +K       EKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD
Sbjct: 768 RWQVKKAIL---EKHGVLPSPEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 827

Query: 781 SAVQGQRTCTNQQ 794
           SAVQGQRTCTNQQ
Sbjct: 828 SAVQGQRTCTNQQ 837

HSP 2 Score: 1058.5 bits (2736), Expect = 5.7e-306
Identity = 546/567 (96.30%), Postives = 550/567 (97.00%), Query Frame = 1

Query: 2   SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI 61
           SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI
Sbjct: 51  SSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREI 110

Query: 62  DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV 121
           DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV
Sbjct: 111 DSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVV 170

Query: 122 EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI 181
           EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI
Sbjct: 171 EAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNI 230

Query: 182 RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ 241
           RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ
Sbjct: 231 RRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQ 290

Query: 242 KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR 301
           KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR
Sbjct: 291 KIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR 350

Query: 302 KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP 361
           KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP
Sbjct: 351 KEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFP 410

Query: 362 NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR 421
           NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR
Sbjct: 411 NDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIR 470

Query: 422 SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS 481
           SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS
Sbjct: 471 SRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCS 530

Query: 482 TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE 541
           TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE
Sbjct: 531 TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE 590

Query: 542 ---GVQLDRMDTDDVDEVRSTPSSKQD 566
              G  L   D DD + +     +K +
Sbjct: 591 EDPGESLSDCDKDDEESLEEEGCAKAE 617

BLAST of Cucsa.277660.6 vs. NCBI nr
Match: gi|700206212|gb|KGN61331.1| (hypothetical protein Csa_2G083770 [Cucumis sativus])

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/253 (94.86%), Postives = 241/253 (95.26%), Query Frame = 1

Query: 541 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 600
           EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL
Sbjct: 637 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 696

Query: 601 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 660
           LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN
Sbjct: 697 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 756

Query: 661 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 720
           GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN
Sbjct: 757 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 816

Query: 721 RWQVCKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 780
           RWQV K IL   +K       EKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD
Sbjct: 817 RWQVKKAIL---EKHGVLPSPEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 876

Query: 781 SAVQGQRTCTNQQ 794
           SAVQGQRTCTNQQ
Sbjct: 877 SAVQGQRTCTNQQ 886

HSP 2 Score: 1049.3 bits (2712), Expect = 3.4e-303
Identity = 541/562 (96.26%), Postives = 545/562 (96.98%), Query Frame = 1

Query: 7   MDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFK 66
           MDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFK
Sbjct: 1   MDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFK 60

Query: 67  YYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 126
           YYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV
Sbjct: 61  YYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 120

Query: 127 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 186
           ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR
Sbjct: 121 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 180

Query: 187 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 246
           KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE
Sbjct: 181 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 240

Query: 247 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEE 306
           MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEE
Sbjct: 241 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEE 300

Query: 307 NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQST 366
           NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQST
Sbjct: 301 NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQST 360

Query: 367 TELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGEK 426
           TELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGEK
Sbjct: 361 TELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGEK 420

Query: 427 HWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTLLDV 486
           HWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTLLDV
Sbjct: 421 HWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTLLDV 480

Query: 487 RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE---GV 546
           RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE   G 
Sbjct: 481 RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEEEDPGE 540

Query: 547 QLDRMDTDDVDEVRSTPSSKQD 566
            L   D DD + +     +K +
Sbjct: 541 SLSDCDKDDEESLEEEGCAKAE 562

BLAST of Cucsa.277660.6 vs. NCBI nr
Match: gi|449442241|ref|XP_004138890.1| (PREDICTED: chromatin assembly factor 1 subunit FAS1 isoform X2 [Cucumis sativus])

HSP 1 Score: 470.3 bits (1209), Expect = 6.6e-129
Identity = 240/253 (94.86%), Postives = 241/253 (95.26%), Query Frame = 1

Query: 541 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 600
           EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL
Sbjct: 582 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 641

Query: 601 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 660
           LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN
Sbjct: 642 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 701

Query: 661 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 720
           GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN
Sbjct: 702 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 761

Query: 721 RWQVCKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 780
           RWQV K IL   +K       EKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD
Sbjct: 762 RWQVKKAIL---EKHGVLPSPEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 821

Query: 781 SAVQGQRTCTNQQ 794
           SAVQGQRTCTNQQ
Sbjct: 822 SAVQGQRTCTNQQ 831

HSP 2 Score: 1018.8 bits (2633), Expect = 5.0e-294
Identity = 521/568 (91.73%), Postives = 543/568 (95.60%), Query Frame = 1

Query: 1   MSSFLGMDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQRE 60
           MSSFLGMDAVVMD+DE SK SSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQ+E
Sbjct: 1   MSSFLGMDAVVMDVDECSKSSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQKE 60

Query: 61  IDSLFKYYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGV 120
           IDSLFKYYDEVKCQKVDLDLG CSSS+SIVAALMEESELSLSKLVDEI+EKM+KIDNGGV
Sbjct: 61  IDSLFKYYDEVKCQKVDLDLGLCSSSNSIVAALMEESELSLSKLVDEIFEKMRKIDNGGV 120

Query: 121 VEAVTVASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILN 180
           +E VTVASVKASVLFVGRRVMYGVPNADADVLEDVS+ECLWCWETRDLKLMPKSTRGILN
Sbjct: 121 LETVTVASVKASVLFVGRRVMYGVPNADADVLEDVSKECLWCWETRDLKLMPKSTRGILN 180

Query: 181 IRRTCRKKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLS 240
           IRRTCRKKIQERVTVLSAMKS+LLK ETDQ+CIQEFTKASD+LSKVFDEAKIRLLTDGLS
Sbjct: 181 IRRTCRKKIQERVTVLSAMKSALLKLETDQSCIQEFTKASDKLSKVFDEAKIRLLTDGLS 240

Query: 241 QKIATEMAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEK 300
           +KIATEMAEKEAKREEKLMVKQLER+QREAEKEKKRIDREQQKEKLQNEKESKVTEREEK
Sbjct: 241 EKIATEMAEKEAKREEKLMVKQLERSQREAEKEKKRIDREQQKEKLQNEKESKVTEREEK 300

Query: 301 RKEKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSF 360
           R+EKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPS SF
Sbjct: 301 RREKEENEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSLSF 360

Query: 361 PNDQSTTELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI 420
           PN+QSTTELIISVPLSK+ ENVL+ACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI
Sbjct: 361 PNEQSTTELIISVPLSKRCENVLEACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSI 420

Query: 421 RSRGEKHWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC 480
           RSRG+KHWGIR+KPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC
Sbjct: 421 RSRGKKHWGIRQKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC 480

Query: 481 STLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE 540
            TLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE
Sbjct: 481 GTLLDVRKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWE 540

Query: 541 E---GVQLDRMDTDDVDEVRSTPSSKQD 566
           E   G  L   D DD + +     +K +
Sbjct: 541 EEDPGESLSDCDKDDEESLEEEGCAKAE 568

BLAST of Cucsa.277660.6 vs. NCBI nr
Match: gi|659082306|ref|XP_008441772.1| (PREDICTED: chromatin assembly factor 1 subunit FAS1 isoform X1 [Cucumis melo])

HSP 1 Score: 442.2 bits (1136), Expect = 1.9e-120
Identity = 225/253 (88.93%), Postives = 236/253 (93.28%), Query Frame = 1

Query: 541 EGVQLDRMDTDDVDEVRSTPSSKQDMEGKELYSVLKQQKHLHNMTNLALRKNQPLIILNL 600
           EGVQLDRMDTDDVDEVRSTPSS+QD+EGKELYSVLKQQKHLHNMT+LALRKNQPLIILNL
Sbjct: 588 EGVQLDRMDTDDVDEVRSTPSSRQDIEGKELYSVLKQQKHLHNMTSLALRKNQPLIILNL 647

Query: 601 LHEKDSLLMAEDLDCTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEVCVPSDKDN 660
           LHEKDSLLMAEDLD TSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPE+C+PSDKDN
Sbjct: 648 LHEKDSLLMAEDLDGTSKLEQTCLAALSMCLMPGGCLIEMSVDGMADEDPEMCIPSDKDN 707

Query: 661 GTQISTSTILDSEMTAIVSTIQSCSQGINKVVESLQLKFPSVPKTHLRNKVREVSDFVEN 720
           GTQISTS ILDSEMTAIVSTIQSCSQGINKVVESLQLKFP+VPK+HLRNKVREVSDFVEN
Sbjct: 708 GTQISTSAILDSEMTAIVSTIQSCSQGINKVVESLQLKFPNVPKSHLRNKVREVSDFVEN 767

Query: 721 RWQVCKRILTFGDKTITHTLLEKGTRRPKTIAAFFSKRCLPPAGKCINPNGSSPQSLEPD 780
           RWQV K IL   +K       EKGTRRPK+IAAFFSKRCLPPAGKCINPN +SPQSL+P 
Sbjct: 768 RWQVKKAIL---EKHGVLPSPEKGTRRPKSIAAFFSKRCLPPAGKCINPNETSPQSLKPG 827

Query: 781 SAVQGQRTCTNQQ 794
           SAVQ QRTCTNQQ
Sbjct: 828 SAVQDQRTCTNQQ 837

HSP 2 Score: 1007.7 bits (2604), Expect = 1.1e-290
Identity = 515/562 (91.64%), Postives = 537/562 (95.55%), Query Frame = 1

Query: 7   MDAVVMDLDESSKPSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQREIDSLFK 66
           MDAVVMD+DE SK SSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQ+EIDSLFK
Sbjct: 1   MDAVVMDVDECSKSSSTDTQARPRKVQKRKRGCMEIVSLEKEEREARIEGIQKEIDSLFK 60

Query: 67  YYDEVKCQKVDLDLGQCSSSDSIVAALMEESELSLSKLVDEIYEKMKKIDNGGVVEAVTV 126
           YYDEVKCQKVDLDLG CSSS+SIVAALMEESELSLSKLVDEI+EKM+KIDNGGV+E VTV
Sbjct: 61  YYDEVKCQKVDLDLGLCSSSNSIVAALMEESELSLSKLVDEIFEKMRKIDNGGVLETVTV 120

Query: 127 ASVKASVLFVGRRVMYGVPNADADVLEDVSRECLWCWETRDLKLMPKSTRGILNIRRTCR 186
           ASVKASVLFVGRRVMYGVPNADADVLEDVS+ECLWCWETRDLKLMPKSTRGILNIRRTCR
Sbjct: 121 ASVKASVLFVGRRVMYGVPNADADVLEDVSKECLWCWETRDLKLMPKSTRGILNIRRTCR 180

Query: 187 KKIQERVTVLSAMKSSLLKSETDQTCIQEFTKASDRLSKVFDEAKIRLLTDGLSQKIATE 246
           KKIQERVTVLSAMKS+LLK ETDQ+CIQEFTKASD+LSKVFDEAKIRLLTDGLS+KIATE
Sbjct: 181 KKIQERVTVLSAMKSALLKLETDQSCIQEFTKASDKLSKVFDEAKIRLLTDGLSEKIATE 240

Query: 247 MAEKEAKREEKLMVKQLERNQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRKEKEE 306
           MAEKEAKREEKLMVKQLER+QREAEKEKKRIDREQQKEKLQNEKESKVTEREEKR+EKEE
Sbjct: 241 MAEKEAKREEKLMVKQLERSQREAEKEKKRIDREQQKEKLQNEKESKVTEREEKRREKEE 300

Query: 307 NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSSSFPNDQST 366
           NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPS SFPN+QST
Sbjct: 301 NEMKKQLRKQQEDAEKEQRRREKEEAEFKKQLSLQKQASIMERFLKKSKPSLSFPNEQST 360

Query: 367 TELIISVPLSKKSENVLQACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGEK 426
           TELIISVPLSK+ ENVL+ACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRG+K
Sbjct: 361 TELIISVPLSKRCENVLEACTQLMDCTLSSSDAIIPVDIRRQHLSSWRLIGSSIRSRGKK 420

Query: 427 HWGIRRKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCSTLLDV 486
           HWGIR+KPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELC TLLDV
Sbjct: 421 HWGIRQKPKSELFKELKLSAGRESANDDELGEERLVDGWEEQITDAGTSQTELCGTLLDV 480

Query: 487 RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE---GV 546
           RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEE   G 
Sbjct: 481 RKSNRGKQLLQFAKSYRPAFYGIWSSKSHVVGPRHPFRKDPDLDYDVDSDEEWEEEDPGE 540

Query: 547 QLDRMDTDDVDEVRSTPSSKQD 566
            L   D DD + +     +K +
Sbjct: 541 SLSDCDKDDEESLEEEGCAKAE 562