Cucsa.271400.1 (mRNA) Cucumber (Gy14) v1

BLAST of Cucsa.271400.1 vs. Swiss-Prot
Match: UBP22_ARATH (Ubiquitin carboxyl-terminal hydrolase 22 OS=Arabidopsis thaliana GN=UBP22 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.1e-188
Identity = 336/556 (60.43%), Postives = 407/556 (73.20%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS R     NP PCNHL+DYKLRYG +GYKS  N  +   + R  I+     +PRC  C 
Sbjct: 1   MSARISFLKNPDPCNHLSDYKLRYGTDGYKSFNNLFRCFNDARIKIKLQGIDIPRCSYCS 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
            Y+ RLYICLIC SISCS+H LLH Q   GHD+A+DVER+ELYC AC DQVYD +FD  V
Sbjct: 61  VYQKRLYICLICRSISCSSHILLHTQLNKGHDIAIDVERSELYCCACIDQVYDSEFDEVV 120

Query: 121 MSKHIVVPEKS-KSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           +SK +     S KS ++ +  RS+K+RRL S  L+   N   L+S +D+R K  +PLGLR
Sbjct: 121 VSKQLFGLGMSVKSGADVVAVRSNKKRRLDS-QLIIGSNF--LVSPRDRREKWTFPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GLNNLG+TCFMN+VLQ  +H PPL N++L+ QHNR+ C +R +G LCLPCD+D IFSA+F
Sbjct: 181 GLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPRRTMGLLCLPCDLDVIFSAMF 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
           SGD  PYSPA  LYSWWQHS NLA YEQQD+HEFFIS+LDRIHE E KS+   ++N +C+
Sbjct: 241 SGDRTPYSPAHLLYSWWQHSTNLATYEQQDSHEFFISLLDRIHENEGKSKCLYQDNEECQ 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-FSSRNIAGKLTK--PADF 360
           CI H+AFSG LRSDVTC TCG TSTTYDP +DISL LD+ + FS  +         P+  
Sbjct: 301 CITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSPADCRKNRYSGGPSVN 360

Query: 361 SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSF 420
           +   TLSGCLD FTR EKLG DQKL CQ+C EKR S KQMSIRRLPL+LCLH+KRFEHS 
Sbjct: 361 AIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSL 420

Query: 421 IRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGD-ELDASS------KFEI 480
            R+TSRKID YLQ+PF L+M PYLSSSII  RFGNRIFA +G+ E D+SS      +FEI
Sbjct: 421 TRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEI 480

Query: 481 FAVVSHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKS 540
           FAVV+H G LESGHYVTYLRL+  WY+CDDAWI EV+EE+VR  +CYM+FY Q+T   K+
Sbjct: 481 FAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKA 540

Query: 541 NEDLSCLPISPRNDAF 546
           +++LS   IS   DAF
Sbjct: 541 HKELSYQVIS-MADAF 552

BLAST of Cucsa.271400.1 vs. Swiss-Prot
Match: UBP51_HUMAN (Ubiquitin carboxyl-terminal hydrolase 51 OS=Homo sapiens GN=USP51 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.0e-88
Identity = 188/524 (35.88%), Postives = 284/524 (54.20%), Query Frame = 1

Query: 11  PTPCNHLADYKLRYGLNGYKSIQN-HLKTSPNGRTSIQNPDSKLPRCGSCDGYKGRLYIC 70
           PT C+H+  +K+  G N  K+++  + +   +G    +   +K   C  C  +  RL+ C
Sbjct: 192 PTGCSHVESFKV--GKNWQKNLRLIYQRFVWSGTPETRKRKAKSCICHVCSTHMNRLHSC 251

Query: 71  LICASISCSNHTLLHYQSENG-HDLAVDVERAELYCYACSDQVYDPDFDRTVMSKHIVVP 130
           L C    C     +H  +E   H LAVD+    +YC+ C D VYD D ++        + 
Sbjct: 252 LSCVFFGCFTEKHIHKHAETKQHHLAVDLYHGVIYCFMCKDYVYDKDIEQIAKETKEKIL 311

Query: 131 EKSKSQSESIGERSSKRRRL-GSVDLLDLKNLKPLI--SVKDQRSKSCYPLGLRGLNNLG 190
               S S  +  +              + K  +P +    K +R KS Y +GLRGL NLG
Sbjct: 312 RLLTSTSTDVSHQQFMTSGFEDKQSTCETKEQEPKLVKPKKKRRKKSVYTVGLRGLINLG 371

Query: 191 NTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFSGDHMP 250
           NTCFMN ++Q   H P L ++FL+D+H     S      LCL C++  +F A++SG   P
Sbjct: 372 NTCFMNCIVQALTHIPLLKDFFLSDKHKCIMTSP----SLCLVCEMSSLFHAMYSGSRTP 431

Query: 251 YSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGD--CKCIAH 310
           + P + L+  W H+ +LA Y QQDAHEF I++LD +H   +      + N    C CI  
Sbjct: 432 HIPYKLLHLIWIHAEHLAGYRQQDAHEFLIAILDVLHRHSKDDSGGQEANNPNCCNCIID 491

Query: 311 RAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDL--DTQDFSSRN---IAGKLTKPADFSS 370
           + F+G L+SDVTC  C   STT DPC DISLDL      F S+N       +++      
Sbjct: 492 QIFTGGLQSDVTCQACHSVSTTIDPCWDISLDLPGSCATFDSQNPERADSTVSRDDHIPG 551

Query: 371 KSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIR 430
             +L+ CL  FTRPE LGS  K+ C +CQ  + S KQ+++++LP+V C H+KRFEH  + 
Sbjct: 552 IPSLTDCLQWFTRPEHLGSSAKIKCNSCQSYQESTKQLTMKKLPIVACFHLKRFEH--VG 611

Query: 431 RTSRKIDRYLQFPFSLDMIPYLSSS-IIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 490
           +  RKI+ ++ FP  LDM P+L+S+   R + G        D +   +K+ +FAV++H G
Sbjct: 612 KQRRKINTFISFPLELDMTPFLASTKESRMKEGQ----PPTDCVPNENKYSLFAVINHHG 671

Query: 491 TLESGHYVTYLR-LRNRWYKCDDAWITEVDEEMVRNSQCYMMFY 521
           TLESGHY +++R  +++W+ CDDA IT+   E +  S+ Y++FY
Sbjct: 672 TLESGHYTSFIRQQKDQWFSCDDAIITKATIEDLLYSEGYLLFY 703

BLAST of Cucsa.271400.1 vs. Swiss-Prot
Match: UBP22_DANRE (Ubiquitin carboxyl-terminal hydrolase 22 OS=Danio rerio GN=usp22 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.0e-88
Identity = 190/524 (36.26%), Postives = 284/524 (54.20%), Query Frame = 1

Query: 10  NPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCDGYKGRLYIC 69
           +P  C+H+  +K+       + I      S +  T  +   S +  C  C  +  RL+ C
Sbjct: 2   SPAGCSHVNGFKVDNWKQNLRVIYQCFVWSGSAETRKRKAKSCI--CHMCGAHLNRLHSC 61

Query: 70  LICASISCSNHTLLHYQSENG-HDLAVDVERAELYCYACSDQVYDPDFDRTVMSKHIVVP 129
           L C    C +   +H  ++N  H+LA+D+    +YC+ C D +YD D ++    +     
Sbjct: 62  LHCVFFGCFSKKHIHEHAKNKRHNLAIDLLYGGIYCFVCQDYIYDKDMEQIAKEE----- 121

Query: 130 EKSKSQSESIGERSS-----KRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRGLNN 189
           ++   + + IGE+ S     KR        L+L    P    K ++  +   +GLRGL N
Sbjct: 122 QRKAWKLQGIGEKYSMWEPTKRE-------LELLRHNP----KRRKITANCTIGLRGLIN 181

Query: 190 LGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFSGDH 249
           LGNTCFMN ++Q   HTP L ++FL+D+H  E  S       CL C++  +F   +SG  
Sbjct: 182 LGNTCFMNCIVQALTHTPLLRDFFLSDRHKCEMQSNS-----CLVCEMSQLFQEFYSGHR 241

Query: 250 MPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGD-CKCIA 309
            P+ P + L+  W H+ +LA YEQQDAHEF I+ LD +H   +    +   N + C CI 
Sbjct: 242 SPHIPFRLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKDDNGKKANNPNHCNCII 301

Query: 310 HRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-----FSSRNIAGKLTKPADFS 369
            + F+G L+SDVTC  C   STT DP  DISLDL          S  +    +   +  S
Sbjct: 302 DQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSDGSVVNGDSHPS 361

Query: 370 SKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFI 429
             +TL+ CL  FTRPE LGS  K+ C  C   + S KQ++++RLP+V C H+KRFEHS  
Sbjct: 362 GATTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKRLPIVACFHLKRFEHS-- 421

Query: 430 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 489
            +  RKI  Y+ FP  LDM P+++SS  +    N  +    D L+  +K+ +FAVV+H G
Sbjct: 422 AKLRRKITTYVSFPLELDMTPFMASS--KESRMNGQYQQPVDSLNNDNKYSLFAVVNHQG 481

Query: 490 TLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFY 521
           TLESGHY T++R  +++W+KCDDA IT+   + V +S+ Y++FY
Sbjct: 482 TLESGHYTTFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFY 498

BLAST of Cucsa.271400.1 vs. Swiss-Prot
Match: UB22B_XENLA (Ubiquitin carboxyl-terminal hydrolase 22-B OS=Xenopus laevis GN=usp22-b PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 5.1e-88
Identity = 194/537 (36.13%), Postives = 285/537 (53.07%), Query Frame = 1

Query: 10  NPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCDGYKGRLYIC 69
           +P  C+H+  +K+       + I      S  G    +   +K   C  C  +  RL+ C
Sbjct: 2   SPAGCSHVNSFKVENWRQNLRVIYQCFVWS--GTPETRKRKAKSCVCHMCGAHLNRLHSC 61

Query: 70  LICASISCSNHTLLHYQSENG-HDLAVDVERAELYCYACSDQVYDPDFDRT--------- 129
           L C    C     +H  ++N  H+LA+D+    +YC+ C D +YD D ++          
Sbjct: 62  LYCVYFGCFTKKHIHEHAKNKRHNLAIDLLYGGIYCFMCQDYIYDKDMEQVAKEEQRKAW 121

Query: 130 ---VMSKHIVVPEKSKSQSESIGERSS-----KRRRLGSVDLLDLKNLKPLISVKDQRSK 189
              V S  +V P +       +GE+ S     KR        L+L    P    K ++  
Sbjct: 122 KLQVFSPALVSPYQYTMTG--VGEKYSTWEPTKRE-------LELLQHNP----KRRKIT 181

Query: 190 SCYPLGLRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDI 249
           +   +GLRGL NLGNTCFMN ++Q   HTP L ++FL+D+H  E  S       CL C++
Sbjct: 182 TNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHKCEMQSPNS----CLVCEM 241

Query: 250 DCIFSAVFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQ 309
             +F   +SG   P+ P + L+  W H+ +LA YEQQDAHEF I+ LD +H   +   + 
Sbjct: 242 STLFQEFYSGHRSPHIPYRLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKGDDNG 301

Query: 310 DKENGD--CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-----FSSR 369
            K N    C CI  + F+G L+SDVTC  C   STT DP  DISLDL          S  
Sbjct: 302 KKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPG 361

Query: 370 NIAGKLTKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLV 429
           + AG +   +  S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ+++++LP+V
Sbjct: 362 SDAGVVNGESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIV 421

Query: 430 LCLHIKRFEHSFIRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDAS 489
            C H+KRFEHS   +  RKI  Y+ FP  LDM+P+++SS  +    N  +    D L   
Sbjct: 422 ACFHLKRFEHS--AKLRRKITTYVSFPLELDMMPFMASS--KESRMNGQYQQPSDSLHND 481

Query: 490 SKFEIFAVVSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFY 521
           +K+ +FAVV+H GTLESGHY +++R  +++W+KCDDA IT+   + V +S+ Y++FY
Sbjct: 482 NKYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVIDSEGYLLFY 515

BLAST of Cucsa.271400.1 vs. Swiss-Prot
Match: UB22A_XENLA (Ubiquitin carboxyl-terminal hydrolase 22-A OS=Xenopus laevis GN=usp22-a PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 6.7e-88
Identity = 194/537 (36.13%), Postives = 284/537 (52.89%), Query Frame = 1

Query: 10  NPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCDGYKGRLYIC 69
           +P  C+H+  +K+       + I      S    T  +   S +  C  C  +  RL+ C
Sbjct: 2   SPAGCSHVNSFKVENWKQNLRVIYQCFVWSGTPETRKRKAKSCI--CHMCGAHLNRLHSC 61

Query: 70  LICASISCSNHTLLHYQSENG-HDLAVDVERAELYCYACSDQVYDPDFDRT--------- 129
           L C    C     +H  ++N  H+LA+D+    +YC+ C D +YD D ++          
Sbjct: 62  LYCVFFGCFTKKHIHEHAKNKRHNLAIDLLYGGIYCFMCQDYIYDKDMEQVAKEEQRKAW 121

Query: 130 ---VMSKHIVVPEKSKSQSESIGERSS-----KRRRLGSVDLLDLKNLKPLISVKDQRSK 189
              V S  +V P +       +GE+ S     KR        L+L    P    K ++  
Sbjct: 122 KLQVFSPALVSPYQYTMTG--VGEKYSTWEPTKRE-------LELLQHNP----KRRKIT 181

Query: 190 SCYPLGLRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDI 249
           +   +GLRGL NLGNTCFMN ++Q   HTP L ++FL+D+H  E  S       CL C++
Sbjct: 182 TNCTIGLRGLINLGNTCFMNCIVQALTHTPLLRDFFLSDRHKCEMQSPNS----CLVCEM 241

Query: 250 DCIFSAVFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQ 309
             +F   +SG   P+ P + L+  W H+ +LA YEQQDAHEF I+ LD +H   +   + 
Sbjct: 242 STLFQEFYSGHRSPHIPYRLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKGDDNG 301

Query: 310 DKENGD--CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-----FSSR 369
            K N    C CI  + F+G L+SDVTC  C   STT DP  DISLDL          S  
Sbjct: 302 KKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPG 361

Query: 370 NIAGKLTKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLV 429
           + AG +   +  S  +TL+ CL  FTRPE LGS  K+ C  C   + S KQ+++++LP+V
Sbjct: 362 SDAGVVNGESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIV 421

Query: 430 LCLHIKRFEHSFIRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDAS 489
            C H+KRFEHS   +  RKI  Y+ FP  LDM P+++SS  +    N  +    D L   
Sbjct: 422 ACFHLKRFEHS--AKLRRKITTYVSFPLELDMTPFMASS--KESRMNGQYQQPSDSLHND 481

Query: 490 SKFEIFAVVSHSGTLESGHYVTYLRL-RNRWYKCDDAWITEVDEEMVRNSQCYMMFY 521
           +K+ +FAVV+H GTLESGHY +++R  +++W+KCDDA IT+   + V +S+ Y++FY
Sbjct: 482 NKYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFY 515

BLAST of Cucsa.271400.1 vs. TrEMBL
Match: M5XFM4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003724mg PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 3.1e-241
Identity = 398/553 (71.97%), Postives = 465/553 (84.09%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS R PSY+NP PC HL+ YKL++GL+GYKS+Q  LKT+PNGRTS+QNP +++PRC SC+
Sbjct: 1   MSTRKPSYTNPKPCKHLSAYKLKHGLSGYKSLQKCLKTTPNGRTSVQNPGTEIPRCSSCN 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GY+GRLYICLIC+SISCS+H LLH QSE GH LAVD+ERAELYC ACSDQVYDPDFD+TV
Sbjct: 61  GYQGRLYICLICSSISCSSHALLHTQSEIGHYLAVDIERAELYCCACSDQVYDPDFDKTV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGS-VDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           MSKH+V    S + ++SIGER  +R+RLGS V+L+DLK  +     +DQR+KSCYPLGLR
Sbjct: 121 MSKHMVDLPYSTNSADSIGERLCERKRLGSGVELVDLKKAEKSALTRDQRAKSCYPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GL NLGNTCFMNSVLQ  LH PPL NYFL+D+HN +TC KR   +LCLPC++  IFSAV+
Sbjct: 181 GLTNLGNTCFMNSVLQALLHAPPLRNYFLSDRHNPKTCRKRTANRLCLPCELHGIFSAVY 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
            GD  PYSPAQFLYSWWQHS NLA YEQQDAHEFFISVLD IHE+E K+R+Q ++ GDC+
Sbjct: 241 LGDRTPYSPAQFLYSWWQHSENLASYEQQDAHEFFISVLDGIHEKESKARNQTRDTGDCQ 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSK 360
           CIAHR FSG LRSDVTCMTCGFTSTTYDPC+DISL+LDT   S  + + K  KP D SS 
Sbjct: 301 CIAHRVFSGLLRSDVTCMTCGFTSTTYDPCLDISLNLDTSHCSWSDASNKPVKPNDNSSS 360

Query: 361 STLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRR 420
           STL GCLDLFT+PEKLGSDQKL+CQNC+E R+S KQMSIR+LPLVLCLHIKRFEHS +R+
Sbjct: 361 STLLGCLDLFTKPEKLGSDQKLYCQNCEELRDSSKQMSIRKLPLVLCLHIKRFEHSLVRK 420

Query: 421 TSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTL 480
             RKID YL FPFSLDM PYLSSSI+RNRFGNRIFA EGDE D S++FEIFAVV+HSGTL
Sbjct: 421 MMRKIDSYLHFPFSLDMTPYLSSSIVRNRFGNRIFAFEGDESDISTEFEIFAVVTHSGTL 480

Query: 481 ESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPIS 540
           ESGHYVTYLR++N+WYKCD+AWI EVDE +VR SQCYMMFY+ K   +KS+EDLS LPIS
Sbjct: 481 ESGHYVTYLRIKNQWYKCDNAWINEVDEGIVRASQCYMMFYVHKMLYYKSHEDLSRLPIS 540

Query: 541 PRNDAFQQIAGCC 553
            R +AF ++AGCC
Sbjct: 541 TRIEAFHRVAGCC 553

BLAST of Cucsa.271400.1 vs. TrEMBL
Match: W9RZG9_9ROSA (Ubiquitin carboxyl-terminal hydrolase 22 OS=Morus notabilis GN=L484_025484 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 3.8e-239
Identity = 394/553 (71.25%), Postives = 462/553 (83.54%), Query Frame = 1

Query: 3   QRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCDGY 62
           + NP Y+NP PC HL+DYKL++G NGYKS+Q  ++T+PNGRTS++NP++K+P C SC+GY
Sbjct: 4   RNNPPYTNPKPCEHLSDYKLKHGFNGYKSLQKFIRTTPNGRTSLENPNTKVPTCDSCNGY 63

Query: 63  KGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTVMS 122
           +GR Y CL+C+SISCS+H L H QS NGHD+AVDVERAEL+C  C DQVYDPDFD+ VMS
Sbjct: 64  RGRFYFCLVCSSISCSDHVLFHNQSRNGHDIAVDVERAELFCIVCRDQVYDPDFDKAVMS 123

Query: 123 KHIVVPEKSKSQSESIGERSSKRRRLGS----VDLL-DLKNLKPLISVKDQRSKSCYPLG 182
           KH +     +S  +SIGER  KR++ GS    VD + DLK  K    ++DQR+KSCYPLG
Sbjct: 124 KHRIDMPNVESDGKSIGERLCKRKKSGSGFGVVDQMRDLKGPKESALMRDQRAKSCYPLG 183

Query: 183 LRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSA 242
           LRGLNNLGNTCFMNSVLQ  LH PPL +YFL +QHNRETC KR + +LCLPCDID IFSA
Sbjct: 184 LRGLNNLGNTCFMNSVLQAMLHAPPLRSYFLTNQHNRETCRKRSVDRLCLPCDIDGIFSA 243

Query: 243 VFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGD 302
           VFSGD  PYSPAQFLYSWWQHSANLA YEQQDAHEFFIS+LD IHE+E K R++++ENGD
Sbjct: 244 VFSGDRPPYSPAQFLYSWWQHSANLASYEQQDAHEFFISLLDGIHEKEGKIRNRNRENGD 303

Query: 303 CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFS 362
           C+CIAHR FSG LRSDVTCMTCGFTSTTYDP VDISL+LDT ++S  ++A K  KP + S
Sbjct: 304 CQCIAHRGFSGLLRSDVTCMTCGFTSTTYDPYVDISLNLDTSNYSMTDVAKKPIKPNESS 363

Query: 363 SKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFI 422
           S STL GCLDLFTRPEKLGSDQKL+CQNCQE+++S KQMSIRRLPLVLCLHIKRFEHS I
Sbjct: 364 SISTLLGCLDLFTRPEKLGSDQKLYCQNCQERQDSSKQMSIRRLPLVLCLHIKRFEHSLI 423

Query: 423 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 482
            + SRKIDRYL FPFSLDM PYLSSSI+RNRFGNRIFA EGDE D S++FEI AVV+HSG
Sbjct: 424 SKMSRKIDRYLHFPFSLDMTPYLSSSIVRNRFGNRIFAFEGDESDISTEFEILAVVTHSG 483

Query: 483 TLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLP 542
           TLESGHYVTYLRLRN+WYKCDDAWITEVDEE+VR SQCYMMFY+QK   HK++E++S L 
Sbjct: 484 TLESGHYVTYLRLRNQWYKCDDAWITEVDEEIVRASQCYMMFYVQKMLYHKADENMSHLT 543

Query: 543 ISPRNDAFQQIAG 551
            S + +A  ++AG
Sbjct: 544 TSSKEEALHRVAG 556

BLAST of Cucsa.271400.1 vs. TrEMBL
Match: F6H350_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06860 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 5.9e-232
Identity = 383/553 (69.26%), Postives = 455/553 (82.28%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS++NPSY+NP PC HLADYKLR+GLNG+K++QN LKT+P GRTS+    S+ PRC  C 
Sbjct: 1   MSKQNPSYTNPKPCKHLADYKLRHGLNGFKTLQNCLKTTPYGRTSVSKHKSETPRCSLCF 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GY+GRLY CLIC+SI+CS+H   H QS +GH++AVDVERAELYC  C DQVYDPDFDR V
Sbjct: 61  GYQGRLYFCLICSSITCSSHANEHSQSHHGHEIAVDVERAELYCCVCRDQVYDPDFDRAV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG 180
           +SKH +    + +  E + E S+KRRRLG    L+ K  K LIS+ D+R+KS +PLGLRG
Sbjct: 121 VSKHFMDMPSNVNGVEGVVEESNKRRRLGLGLELEWKRSKRLISMADRRAKSIFPLGLRG 180

Query: 181 LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS 240
           LNNLGNTCFMNSVLQ   H PPL NYFL+D+HNR++C +R   +LCLPCDID IFSAVFS
Sbjct: 181 LNNLGNTCFMNSVLQALFHAPPLRNYFLSDRHNRQSCRRRSSDRLCLPCDIDVIFSAVFS 240

Query: 241 GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC 300
           GD  PYSPA+FLYSWWQHS NLA YEQQDAHEFFIS+LD+IH +E KS +QDK+N DC+C
Sbjct: 241 GDRTPYSPARFLYSWWQHSENLASYEQQDAHEFFISMLDQIHVKEGKSGNQDKDNRDCQC 300

Query: 301 IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSKS 360
           IAH+AFSG LRSDVTC TCGFTSTTYDPCVDISL+LD     S  +  + TKP + +  S
Sbjct: 301 IAHKAFSGLLRSDVTCTTCGFTSTTYDPCVDISLNLDMSFCPSTGMTNRTTKPNENAIIS 360

Query: 361 TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT 420
           TL GCLDLFTRPEKLG DQKL+CQNCQE+RNS+KQMSIRRLP+VLCLHIKRFEHS IR+ 
Sbjct: 361 TLLGCLDLFTRPEKLGPDQKLYCQNCQERRNSLKQMSIRRLPIVLCLHIKRFEHSTIRKM 420

Query: 421 SRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE 480
           SRKID+YLQFPFSLDM PYLSSSI+RNRFGNRIF  EGDE +  ++FE+FAVV+HSG LE
Sbjct: 421 SRKIDQYLQFPFSLDMTPYLSSSIVRNRFGNRIFTFEGDESETCTEFEVFAVVTHSGMLE 480

Query: 481 SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPIS- 540
           SGHYVTY+R+RNRWYKCDDAWITEVDE +VR SQCY+M+Y+QK   +K++EDLSCLP+S 
Sbjct: 481 SGHYVTYVRVRNRWYKCDDAWITEVDEGVVRASQCYLMYYVQKGFYYKASEDLSCLPMSP 540

Query: 541 PRNDAFQQIAGCC 553
           P  +++  IAGCC
Sbjct: 541 PGGESYLPIAGCC 553

BLAST of Cucsa.271400.1 vs. TrEMBL
Match: B9IL79_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02760g PE=3 SV=2)

HSP 1 Score: 808.5 bits (2087), Expect = 5.0e-231
Identity = 388/555 (69.91%), Postives = 459/555 (82.70%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           M+ RN  ++NP PC HLADYKLR+GL+GYKSIQ  LK SP G+TSI+  ++K+P C  C 
Sbjct: 1   MATRNSVFANPKPCEHLADYKLRHGLSGYKSIQKWLKASPYGKTSIKMCETKMPTCRFCS 60

Query: 61  GY-KGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRT 120
           G+ +GRL++CLIC+S+SCS+HTLLH QSENGHD+AVD+ER+ELYC  CSDQVYDPDFD+ 
Sbjct: 61  GFHQGRLFLCLICSSMSCSDHTLLHAQSENGHDIAVDIERSELYCCLCSDQVYDPDFDKV 120

Query: 121 VMSKHIV-VPEKSK-SQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLG 180
           V+SK+I+ +P K+  S  + +  RSSKRRRL  V  LDLK  K L+S+ D+R+KSCYPLG
Sbjct: 121 VVSKNIMDMPSKTHDSVVDDLMRRSSKRRRLNPVVDLDLKRSKLLVSMMDRRAKSCYPLG 180

Query: 181 LRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSA 240
           LRGLNNLG+TCFMNSVLQ  LH PP  NYFL+++H+ ETC KR   QLCL CD   IFSA
Sbjct: 181 LRGLNNLGSTCFMNSVLQALLHAPPFRNYFLSERHDPETCKKRSSDQLCLACDFGVIFSA 240

Query: 241 VFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGD 300
           V+SGD  PYSPAQFLYSWWQHSANLA YEQQDAHEFFISVLD IHERE K RS +K+NG 
Sbjct: 241 VYSGDRTPYSPAQFLYSWWQHSANLASYEQQDAHEFFISVLDGIHEREGKERSSNKDNGG 300

Query: 301 CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFS 360
           C+CIAHR FSG LRSDVTC TCGFTSTTYDPCVDISL++DT +FS+ N+A K  +  + +
Sbjct: 301 CQCIAHRVFSGMLRSDVTCTTCGFTSTTYDPCVDISLNMDTTNFSTVNVANKSVR--EDT 360

Query: 361 SKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFI 420
            +STL  CLDLFTRPE+LGSDQKL+CQNCQEKR+S+KQMSI+RLPLVL LHIKRFEHS +
Sbjct: 361 GRSTLLACLDLFTRPERLGSDQKLYCQNCQEKRDSLKQMSIKRLPLVLSLHIKRFEHSSL 420

Query: 421 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 480
           RR+SRKID YLQFPFSLDM PYLS SI+RNRFGNR+F  E D+ D SS+FEIFAVV+HSG
Sbjct: 421 RRSSRKIDWYLQFPFSLDMTPYLSPSIVRNRFGNRVFGFESDKPDISSEFEIFAVVTHSG 480

Query: 481 TLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLP 540
            L+SGHYVTYLRL+N+WYKCDDAWITEVDE +VR +QCYM+FY+QK    K+NED SC+P
Sbjct: 481 MLDSGHYVTYLRLQNQWYKCDDAWITEVDERIVRAAQCYMIFYVQKMLYCKANEDWSCMP 540

Query: 541 ISPRNDAFQQIAGCC 553
           IS R D F  IAGCC
Sbjct: 541 ISTRRDPFVPIAGCC 553

BLAST of Cucsa.271400.1 vs. TrEMBL
Match: V4WAW8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10007902mg PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 3.2e-230
Identity = 378/553 (68.35%), Postives = 448/553 (81.01%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           M  RN  Y+NP PC HLADYKLR+GL+GY  +Q  ++T+P+G++S++N  +++PRC  C+
Sbjct: 1   MMSRNSMYTNPKPCKHLADYKLRHGLSGYNLLQEQIRTTPSGKSSVKNHQTEIPRCCFCN 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GY+ RLY+CLIC+SISCS+HTLLH Q ENGHD+AVD+ER+ELYC  C DQVYDPDFD+ V
Sbjct: 61  GYQKRLYLCLICSSISCSSHTLLHSQCENGHDIAVDIERSELYCVLCCDQVYDPDFDKIV 120

Query: 121 MSKHIV-VPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           M KH++ +P        S G RSSKRRRL S   LDLK  K L+S++D R+KSCYPLGLR
Sbjct: 121 MLKHLMDLPRSGNGVDVSAGRRSSKRRRLCSALELDLKKTKQLVSMRDLRAKSCYPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GLNNLG+TCFMNSVLQ  LH PP  NYFL  Q N E C KR   +LCL CDI   FSAVF
Sbjct: 181 GLNNLGSTCFMNSVLQALLHAPPFRNYFLTGQRNHEVCRKRSSDRLCLLCDIYVFFSAVF 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
           SGD  PYSPAQ LYSWWQHSANLA YEQQDAHEFFISVLD IHE   K+RS  K+ G+C 
Sbjct: 241 SGDRTPYSPAQLLYSWWQHSANLASYEQQDAHEFFISVLDGIHENGVKARSPIKDTGECH 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSK 360
           C+AHR FSGQLRSDVTCM+CG+TSTTYDP +DISL+LDT +  S ++A K  KP+D +  
Sbjct: 301 CVAHRVFSGQLRSDVTCMSCGYTSTTYDPFMDISLNLDTGNCPSTDLANKSLKPSDNTYV 360

Query: 361 STLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRR 420
           STL GCLDLFT+PEKLGSDQK FC+NCQE+++SVKQMSI+RLPLVLCLHIKRFEH  +RR
Sbjct: 361 STLLGCLDLFTKPEKLGSDQKFFCENCQERQDSVKQMSIKRLPLVLCLHIKRFEHFPVRR 420

Query: 421 TSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTL 480
            SRKIDRY+QFPF LDM PYLSSS++RNRFGNR+FA EGDELD S++FEIFAVV+HSGTL
Sbjct: 421 MSRKIDRYMQFPFCLDMNPYLSSSVVRNRFGNRMFAFEGDELDTSTEFEIFAVVTHSGTL 480

Query: 481 ESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPIS 540
           +SGHYVTYL L+++WYKCDDAWIT V+EE+VR SQCYM+FY QK   +K+NEDL C P+S
Sbjct: 481 DSGHYVTYLHLKDQWYKCDDAWITAVEEEVVRASQCYMLFYAQKMLYYKANEDLVCQPVS 540

Query: 541 PRNDAFQQIAGCC 553
           PR D F  IAGCC
Sbjct: 541 PRADPFLPIAGCC 553

BLAST of Cucsa.271400.1 vs. TAIR10
Match: AT5G10790.1 (AT5G10790.1 ubiquitin-specific protease 22)

HSP 1 Score: 661.0 bits (1704), Expect = 6.5e-190
Identity = 336/556 (60.43%), Postives = 407/556 (73.20%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS R     NP PCNHL+DYKLRYG +GYKS  N  +   + R  I+     +PRC  C 
Sbjct: 1   MSARISFLKNPDPCNHLSDYKLRYGTDGYKSFNNLFRCFNDARIKIKLQGIDIPRCSYCS 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
            Y+ RLYICLIC SISCS+H LLH Q   GHD+A+DVER+ELYC AC DQVYD +FD  V
Sbjct: 61  VYQKRLYICLICRSISCSSHILLHTQLNKGHDIAIDVERSELYCCACIDQVYDSEFDEVV 120

Query: 121 MSKHIVVPEKS-KSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           +SK +     S KS ++ +  RS+K+RRL S  L+   N   L+S +D+R K  +PLGLR
Sbjct: 121 VSKQLFGLGMSVKSGADVVAVRSNKKRRLDS-QLIIGSNF--LVSPRDRREKWTFPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GLNNLG+TCFMN+VLQ  +H PPL N++L+ QHNR+ C +R +G LCLPCD+D IFSA+F
Sbjct: 181 GLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPRRTMGLLCLPCDLDVIFSAMF 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
           SGD  PYSPA  LYSWWQHS NLA YEQQD+HEFFIS+LDRIHE E KS+   ++N +C+
Sbjct: 241 SGDRTPYSPAHLLYSWWQHSTNLATYEQQDSHEFFISLLDRIHENEGKSKCLYQDNEECQ 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQD-FSSRNIAGKLTK--PADF 360
           CI H+AFSG LRSDVTC TCG TSTTYDP +DISL LD+ + FS  +         P+  
Sbjct: 301 CITHKAFSGLLRSDVTCTTCGSTSTTYDPFIDISLTLDSMNGFSPADCRKNRYSGGPSVN 360

Query: 361 SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSF 420
           +   TLSGCLD FTR EKLG DQKL CQ+C EKR S KQMSIRRLPL+LCLH+KRFEHS 
Sbjct: 361 AIMPTLSGCLDFFTRSEKLGPDQKLNCQSCGEKRESSKQMSIRRLPLLLCLHVKRFEHSL 420

Query: 421 IRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGD-ELDASS------KFEI 480
            R+TSRKID YLQ+PF L+M PYLSSSII  RFGNRIFA +G+ E D+SS      +FEI
Sbjct: 421 TRKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEI 480

Query: 481 FAVVSHSGTLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKS 540
           FAVV+H G LESGHYVTYLRL+  WY+CDDAWI EV+EE+VR  +CYM+FY Q+T   K+
Sbjct: 481 FAVVTHKGMLESGHYVTYLRLKGLWYRCDDAWINEVEEEVVRGCECYMLFYAQETVIQKA 540

Query: 541 NEDLSCLPISPRNDAF 546
           +++LS   IS   DAF
Sbjct: 541 HKELSYQVIS-MADAF 552

BLAST of Cucsa.271400.1 vs. TAIR10
Match: AT4G17895.1 (AT4G17895.1 ubiquitin-specific protease 20)

HSP 1 Score: 156.8 bits (395), Expect = 4.0e-38
Identity = 106/348 (30.46%), Postives = 173/348 (49.71%), Query Frame = 1

Query: 180 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 239
           GL NLGN+CF+NSV Q F HT PL    L+ ++             C+   I     A  
Sbjct: 177 GLWNLGNSCFLNSVFQCFTHTVPLIESLLSFRYEVPCHCGNEF--FCVIRAIRYHIEAAL 236

Query: 240 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIH--ERERKSRSQDKENGD 299
             +  P +P  F  +    S +   Y+Q+DAHEF  + L+++     +R S   D  + D
Sbjct: 237 RPERCPIAPYFFFDNLNYFSPDFQRYQQEDAHEFLQAFLEKLEICGSDRTSFRGDITSQD 296

Query: 300 CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFS 359
                   FSG+L S + C  C + S TY+  V +SL+++  D                 
Sbjct: 297 -------VFSGRLISGLRCCNCDYVSETYEKSVGLSLEIEDVD----------------- 356

Query: 360 SKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFI 419
              TL   L+ FTR EKL  D++L C NC EK +  KQ+ + +LPLV   H+KRF+++ +
Sbjct: 357 ---TLGSALESFTRVEKL--DEQLTCDNCNEKVSKEKQLLLDKLPLVATFHLKRFKNNGL 416

Query: 420 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 479
                KI ++++ P  +D+ PY+ +                 E + S+K+ ++A+V H G
Sbjct: 417 --YMEKIYKHVKIPLEIDLQPYMRNI---------------QENEVSTKYHLYALVEHFG 476

Query: 480 -TLESGHYVTYLRLRNR-WYKCDDAWITEVDEEMVRNSQCYMMFYLQK 524
            ++  GHY +Y+R   + W+  DD+ +T +DE+MV +   Y++FY ++
Sbjct: 477 YSVAYGHYSSYVRSAPKIWHHFDDSKVTRIDEDMVLSQDSYILFYARE 476

BLAST of Cucsa.271400.1 vs. TAIR10
Match: AT5G57990.1 (AT5G57990.1 ubiquitin-specific protease 23)

HSP 1 Score: 153.7 bits (387), Expect = 3.4e-37
Identity = 103/348 (29.60%), Postives = 163/348 (46.84%), Query Frame = 1

Query: 180 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 239
           GL NLGNTCF+NSVLQ   +T PL+       H +       +   C  C I        
Sbjct: 108 GLQNLGNTCFLNSVLQCLTYTEPLAATLQTAAHQKYC----HVAGFCALCAIQKHVRTAR 167

Query: 240 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDC- 299
             +    +P   + +    S N     Q+DAHE+ I++L+ +H+    S     E+ D  
Sbjct: 168 QANGRILAPKDLVSNLRCISRNFRNCRQEDAHEYMINLLECMHKCSLPS-GVPSESSDAY 227

Query: 300 -KCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFS 359
            + + H+ F G LRS V C  C   S  +DP +D+SLD+   D                 
Sbjct: 228 RRSLVHKIFGGSLRSQVKCEQCSHCSNKFDPFLDLSLDISKAD----------------- 287

Query: 360 SKSTLSGCLDLFTRPEKLGSDQKLF-CQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSF 419
              +L   L  FT  E L +  K++ C+ C++K  + KQ+++ + P VL +H+KRFE   
Sbjct: 288 ---SLQRALSRFTAVELLDNGAKVYQCERCKQKVKAKKQLTVSKAPYVLTVHLKRFE--- 347

Query: 420 IRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHS 479
               S KIDR + F  ++DM P++S                    + + K+ ++ V+ H 
Sbjct: 348 -AHRSEKIDRKVDFTSAIDMKPFVSG-----------------PHEGNLKYTLYGVLVHY 407

Query: 480 G-TLESGHYVTYLRLRN-RWYKCDDAWITEVDEEMVRNSQCYMMFYLQ 523
           G +  SGHY  ++R  +  WY  DD  + +V E+ V N + YM+FY++
Sbjct: 408 GRSSHSGHYACFVRTSSGMWYSLDDNRVVQVSEKTVFNQKAYMLFYVR 409

BLAST of Cucsa.271400.1 vs. TAIR10
Match: AT4G24560.1 (AT4G24560.1 ubiquitin-specific protease 16)

HSP 1 Score: 145.2 bits (365), Expect = 1.2e-34
Identity = 99/351 (28.21%), Postives = 158/351 (45.01%), Query Frame = 1

Query: 180 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 239
           GL N+GN+CF N V Q  + TPPL+ YFL   H+R  C+K+   + C  C  + +     
Sbjct: 543 GLINVGNSCFANVVFQCLMFTPPLTTYFLQQFHSR-ACTKK---EQCFTCGFEKLVVKA- 602

Query: 240 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENG--- 299
             +  P SP   L         L   +++DAHEF   V+D +     K+   D       
Sbjct: 603 KEEKSPLSPNGLLSQLQNIGIFLGNGKEEDAHEFLRFVVDTMQSVCIKASEYDMTKSSKL 662

Query: 300 DCKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADF 359
           +   +    F G LRS + CM C   S   +  +D+++++D                   
Sbjct: 663 EDTTLIGLTFGGYLRSKIKCMKCQVKSELREKMMDLTVEID------------------- 722

Query: 360 SSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSF 419
              STL   L  FTR E L  + K  C +C+    + K++ I   P VL + +KRF+   
Sbjct: 723 GDISTLDDALRRFTRTEILDGENKYRCGSCKSYERAKKKLKITEPPNVLTIALKRFQAGK 782

Query: 420 IRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHS 479
                 K+++ ++FP +LD+ PY+S                    + S  ++++ V+ H 
Sbjct: 783 FG----KLNKLIRFPETLDLAPYVSGGS-----------------EKSHDYKLYGVIVHL 842

Query: 480 GTLE---SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKT 525
             +    SGHYV Y+R +N+WYK DD+ +   D E +     YM+FY + T
Sbjct: 843 DVMNAAFSGHYVCYIRNQNKWYKADDSTVVTSDVERILTKGAYMLFYARCT 848

BLAST of Cucsa.271400.1 vs. TAIR10
Match: AT1G17110.2 (AT1G17110.2 ubiquitin-specific protease 15)

HSP 1 Score: 139.8 bits (351), Expect = 5.0e-33
Identity = 124/453 (27.37%), Postives = 194/453 (42.83%), Query Frame = 1

Query: 118 RTVMSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLK-------PLISVKDQRS 177
           +TV SK   + E+S   S ++G       R  +      KNLK       P         
Sbjct: 374 KTVRSKDTGISEESNGISSTMGIMKMMGLRNSTKHDDRYKNLKVSSLMLFPYEEFLKFFQ 433

Query: 178 KSCYPLGLRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCD 237
              + L  RGL N GN+C+ N+VLQ    T PL  Y L   H+R +CS +     CL C+
Sbjct: 434 CEVFDLSPRGLVNCGNSCYANAVLQSLTCTKPLVAYLLRRSHSR-SCSGK---DWCLMCE 493

Query: 238 ID-CIFSAVFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSR 297
           ++  +     SG   P S ++ L      +  +    Q+DAHEF      R+     +S 
Sbjct: 494 LEQHVMMLRESGG--PLSASRILSHMRSINCQIGDGSQEDAHEFL-----RLLVASMQSI 553

Query: 298 SQDKENGDCK--------CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDF 357
             ++  G+ K         +    F G+LRS V C+ C   S  Y+  +D++L++     
Sbjct: 554 CLERLGGETKVDPRLQETTLVQHMFGGRLRSKVKCLRCDHESERYENIMDLTLEI----- 613

Query: 358 SSRNIAGKLTKPADFSSKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRL 417
                         +    +L   L  FTRPE L  +    C  C     + K++SI   
Sbjct: 614 --------------YGWVESLQDALTQFTRPEDLDGENMYRCSRCAGYVRARKELSIHEA 673

Query: 418 PLVLCLHIKRFEHSFIRRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDEL 477
           P +L + +KRF+         KI++ + FP  LDMIP+++      R G           
Sbjct: 674 PNILTIVLKRFQEG----RYGKINKCISFPEMLDMIPFMT------RTG----------- 733

Query: 478 DASSKFEIFAVVSHSGTLE---SGHYVTYLR-LRNRWYKCDDAWITEVDEEMVRNSQCYM 537
           D    + ++AV+ H  TL    SGHY++Y++ LR  WY+ DD+ I  V    V +   YM
Sbjct: 734 DVPPLYMLYAVIVHLDTLNASFSGHYISYVKDLRGNWYRIDDSEIHPVPMTQVMSEGAYM 775

Query: 538 MFYLQKTASHKSNEDLSCLPI---SPRNDAFQQ 548
           +FY++     +  E     P+    PRN+  +Q
Sbjct: 794 LFYMRSYPRPQRGEHNGKAPVHHSQPRNEMKEQ 775

BLAST of Cucsa.271400.1 vs. NCBI nr
Match: gi|778696869|ref|XP_011654218.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22 [Cucumis sativus])

HSP 1 Score: 1151.0 bits (2976), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD
Sbjct: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV
Sbjct: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG 180
           MSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG
Sbjct: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG 180

Query: 181 LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS 240
           LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS
Sbjct: 181 LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS 240

Query: 241 GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC 300
           GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC
Sbjct: 241 GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC 300

Query: 301 IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSKS 360
           IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSKS
Sbjct: 301 IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSKS 360

Query: 361 TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT 420
           TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT
Sbjct: 361 TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT 420

Query: 421 SRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE 480
           SRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE
Sbjct: 421 SRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE 480

Query: 481 SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPISP 540
           SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPISP
Sbjct: 481 SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPISP 540

Query: 541 RNDAFQQIAGCC 553
           RNDAFQQIAGCC
Sbjct: 541 RNDAFQQIAGCC 552

BLAST of Cucsa.271400.1 vs. NCBI nr
Match: gi|659104153|ref|XP_008452825.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22 [Cucumis melo])

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 545/552 (98.73%), Postives = 548/552 (99.28%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD
Sbjct: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV
Sbjct: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG 180
           MSKHIVVPEKS+SQS S GERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG
Sbjct: 121 MSKHIVVPEKSQSQSGSTGERSSKRRRLGSVDLLDLKNLKPLISVKDQRSKSCYPLGLRG 180

Query: 181 LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS 240
           LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS
Sbjct: 181 LNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVFS 240

Query: 241 GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC 300
           GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC
Sbjct: 241 GDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCKC 300

Query: 301 IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSKS 360
           IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGK TKPADFSSKS
Sbjct: 301 IAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKSTKPADFSSKS 360

Query: 361 TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT 420
           TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT
Sbjct: 361 TLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRRT 420

Query: 421 SRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE 480
           SRKIDRYLQFPF+LDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE
Sbjct: 421 SRKIDRYLQFPFALDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTLE 480

Query: 481 SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPISP 540
           SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQK A+HKSNEDLSCLPISP
Sbjct: 481 SGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKIANHKSNEDLSCLPISP 540

Query: 541 RNDAFQQIAGCC 553
           RNDAFQQIAGCC
Sbjct: 541 RNDAFQQIAGCC 552

BLAST of Cucsa.271400.1 vs. NCBI nr
Match: gi|596170155|ref|XP_007223067.1| (hypothetical protein PRUPE_ppa003724mg [Prunus persica])

HSP 1 Score: 842.4 bits (2175), Expect = 4.4e-241
Identity = 398/553 (71.97%), Postives = 465/553 (84.09%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS R PSY+NP PC HL+ YKL++GL+GYKS+Q  LKT+PNGRTS+QNP +++PRC SC+
Sbjct: 1   MSTRKPSYTNPKPCKHLSAYKLKHGLSGYKSLQKCLKTTPNGRTSVQNPGTEIPRCSSCN 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GY+GRLYICLIC+SISCS+H LLH QSE GH LAVD+ERAELYC ACSDQVYDPDFD+TV
Sbjct: 61  GYQGRLYICLICSSISCSSHALLHTQSEIGHYLAVDIERAELYCCACSDQVYDPDFDKTV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGS-VDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           MSKH+V    S + ++SIGER  +R+RLGS V+L+DLK  +     +DQR+KSCYPLGLR
Sbjct: 121 MSKHMVDLPYSTNSADSIGERLCERKRLGSGVELVDLKKAEKSALTRDQRAKSCYPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GL NLGNTCFMNSVLQ  LH PPL NYFL+D+HN +TC KR   +LCLPC++  IFSAV+
Sbjct: 181 GLTNLGNTCFMNSVLQALLHAPPLRNYFLSDRHNPKTCRKRTANRLCLPCELHGIFSAVY 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
            GD  PYSPAQFLYSWWQHS NLA YEQQDAHEFFISVLD IHE+E K+R+Q ++ GDC+
Sbjct: 241 LGDRTPYSPAQFLYSWWQHSENLASYEQQDAHEFFISVLDGIHEKESKARNQTRDTGDCQ 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSK 360
           CIAHR FSG LRSDVTCMTCGFTSTTYDPC+DISL+LDT   S  + + K  KP D SS 
Sbjct: 301 CIAHRVFSGLLRSDVTCMTCGFTSTTYDPCLDISLNLDTSHCSWSDASNKPVKPNDNSSS 360

Query: 361 STLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRR 420
           STL GCLDLFT+PEKLGSDQKL+CQNC+E R+S KQMSIR+LPLVLCLHIKRFEHS +R+
Sbjct: 361 STLLGCLDLFTKPEKLGSDQKLYCQNCEELRDSSKQMSIRKLPLVLCLHIKRFEHSLVRK 420

Query: 421 TSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTL 480
             RKID YL FPFSLDM PYLSSSI+RNRFGNRIFA EGDE D S++FEIFAVV+HSGTL
Sbjct: 421 MMRKIDSYLHFPFSLDMTPYLSSSIVRNRFGNRIFAFEGDESDISTEFEIFAVVTHSGTL 480

Query: 481 ESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPIS 540
           ESGHYVTYLR++N+WYKCD+AWI EVDE +VR SQCYMMFY+ K   +KS+EDLS LPIS
Sbjct: 481 ESGHYVTYLRIKNQWYKCDNAWINEVDEGIVRASQCYMMFYVHKMLYYKSHEDLSRLPIS 540

Query: 541 PRNDAFQQIAGCC 553
            R +AF ++AGCC
Sbjct: 541 TRIEAFHRVAGCC 553

BLAST of Cucsa.271400.1 vs. NCBI nr
Match: gi|645227712|ref|XP_008220648.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 22 [Prunus mume])

HSP 1 Score: 842.0 bits (2174), Expect = 5.8e-241
Identity = 399/553 (72.15%), Postives = 465/553 (84.09%), Query Frame = 1

Query: 1   MSQRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCD 60
           MS R PSY+NP PC HL+ YKL++GL+GYKS+Q  LKT+PNGRTS+QNP +++PRC  C+
Sbjct: 1   MSTRKPSYTNPKPCKHLSAYKLKHGLSGYKSLQKCLKTTPNGRTSVQNPGTEVPRCSFCN 60

Query: 61  GYKGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTV 120
           GY+GRLYICLIC+SISCS+H LLH QSE GH LAVDVERAELYC ACSDQVYDPDFD+TV
Sbjct: 61  GYQGRLYICLICSSISCSSHALLHTQSEIGHYLAVDVERAELYCCACSDQVYDPDFDKTV 120

Query: 121 MSKHIVVPEKSKSQSESIGERSSKRRRLGS-VDLLDLKNLKPLISVKDQRSKSCYPLGLR 180
           MSKH+V    S + ++SIGER  +R+RLGS V+L+DLK  +     +DQR+KSCYPLGLR
Sbjct: 121 MSKHMVDLPYSTNSADSIGERLCERKRLGSGVELVDLKKAEKSAWTRDQRAKSCYPLGLR 180

Query: 181 GLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSAVF 240
           GLNNLGNTCFMNSVLQ  LH PPL NYFL+D+HN +TC KR   +LCLPC++  IFSAV+
Sbjct: 181 GLNNLGNTCFMNSVLQALLHAPPLRNYFLSDRHNPKTCRKRTANRLCLPCELHGIFSAVY 240

Query: 241 SGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGDCK 300
            GD  PYSPAQFLYSWWQHS NLA YEQQDAHEFFISVLD IHE+E K+R+Q ++ GDC+
Sbjct: 241 LGDRTPYSPAQFLYSWWQHSENLASYEQQDAHEFFISVLDGIHEKESKARNQTRDTGDCQ 300

Query: 301 CIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFSSK 360
           CIAHR FSG LRSDVTCMTCGFTSTTYDPC+DISL+LDT   S  + + K  KP D SS 
Sbjct: 301 CIAHRVFSGLLRSDVTCMTCGFTSTTYDPCLDISLNLDTSHCSWSDASNKPVKPNDNSSS 360

Query: 361 STLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFIRR 420
           STL GCLDLFT+PEKLGSDQKL+CQNC+E R+S KQMSIR+LPLVLCLHIKRFEHS +R+
Sbjct: 361 STLLGCLDLFTKPEKLGSDQKLYCQNCEELRDSSKQMSIRKLPLVLCLHIKRFEHSLVRK 420

Query: 421 TSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSGTL 480
             RKID YL FPFSLDM PYLSSSI+RNRFGNRIFA EGDE D S++FEIFAVV+HSGTL
Sbjct: 421 MMRKIDSYLHFPFSLDMTPYLSSSIVRNRFGNRIFAFEGDESDISTEFEIFAVVTHSGTL 480

Query: 481 ESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLPIS 540
           ESGHYVTYLR++N+WYKCD+AWI EVDE +VR SQCYMMFY+ K   +KS+EDLS LPIS
Sbjct: 481 ESGHYVTYLRIKNQWYKCDNAWINEVDEGIVRASQCYMMFYVHKMLYYKSHEDLSRLPIS 540

Query: 541 PRNDAFQQIAGCC 553
            R +AF ++AGCC
Sbjct: 541 TRIEAFHRVAGCC 553

BLAST of Cucsa.271400.1 vs. NCBI nr
Match: gi|703130052|ref|XP_010104512.1| (Ubiquitin carboxyl-terminal hydrolase 22 [Morus notabilis])

HSP 1 Score: 835.5 bits (2157), Expect = 5.4e-239
Identity = 394/553 (71.25%), Postives = 462/553 (83.54%), Query Frame = 1

Query: 3   QRNPSYSNPTPCNHLADYKLRYGLNGYKSIQNHLKTSPNGRTSIQNPDSKLPRCGSCDGY 62
           + NP Y+NP PC HL+DYKL++G NGYKS+Q  ++T+PNGRTS++NP++K+P C SC+GY
Sbjct: 4   RNNPPYTNPKPCEHLSDYKLKHGFNGYKSLQKFIRTTPNGRTSLENPNTKVPTCDSCNGY 63

Query: 63  KGRLYICLICASISCSNHTLLHYQSENGHDLAVDVERAELYCYACSDQVYDPDFDRTVMS 122
           +GR Y CL+C+SISCS+H L H QS NGHD+AVDVERAEL+C  C DQVYDPDFD+ VMS
Sbjct: 64  RGRFYFCLVCSSISCSDHVLFHNQSRNGHDIAVDVERAELFCIVCRDQVYDPDFDKAVMS 123

Query: 123 KHIVVPEKSKSQSESIGERSSKRRRLGS----VDLL-DLKNLKPLISVKDQRSKSCYPLG 182
           KH +     +S  +SIGER  KR++ GS    VD + DLK  K    ++DQR+KSCYPLG
Sbjct: 124 KHRIDMPNVESDGKSIGERLCKRKKSGSGFGVVDQMRDLKGPKESALMRDQRAKSCYPLG 183

Query: 183 LRGLNNLGNTCFMNSVLQVFLHTPPLSNYFLNDQHNRETCSKRPIGQLCLPCDIDCIFSA 242
           LRGLNNLGNTCFMNSVLQ  LH PPL +YFL +QHNRETC KR + +LCLPCDID IFSA
Sbjct: 184 LRGLNNLGNTCFMNSVLQAMLHAPPLRSYFLTNQHNRETCRKRSVDRLCLPCDIDGIFSA 243

Query: 243 VFSGDHMPYSPAQFLYSWWQHSANLACYEQQDAHEFFISVLDRIHERERKSRSQDKENGD 302
           VFSGD  PYSPAQFLYSWWQHSANLA YEQQDAHEFFIS+LD IHE+E K R++++ENGD
Sbjct: 244 VFSGDRPPYSPAQFLYSWWQHSANLASYEQQDAHEFFISLLDGIHEKEGKIRNRNRENGD 303

Query: 303 CKCIAHRAFSGQLRSDVTCMTCGFTSTTYDPCVDISLDLDTQDFSSRNIAGKLTKPADFS 362
           C+CIAHR FSG LRSDVTCMTCGFTSTTYDP VDISL+LDT ++S  ++A K  KP + S
Sbjct: 304 CQCIAHRGFSGLLRSDVTCMTCGFTSTTYDPYVDISLNLDTSNYSMTDVAKKPIKPNESS 363

Query: 363 SKSTLSGCLDLFTRPEKLGSDQKLFCQNCQEKRNSVKQMSIRRLPLVLCLHIKRFEHSFI 422
           S STL GCLDLFTRPEKLGSDQKL+CQNCQE+++S KQMSIRRLPLVLCLHIKRFEHS I
Sbjct: 364 SISTLLGCLDLFTRPEKLGSDQKLYCQNCQERQDSSKQMSIRRLPLVLCLHIKRFEHSLI 423

Query: 423 RRTSRKIDRYLQFPFSLDMIPYLSSSIIRNRFGNRIFASEGDELDASSKFEIFAVVSHSG 482
            + SRKIDRYL FPFSLDM PYLSSSI+RNRFGNRIFA EGDE D S++FEI AVV+HSG
Sbjct: 424 SKMSRKIDRYLHFPFSLDMTPYLSSSIVRNRFGNRIFAFEGDESDISTEFEILAVVTHSG 483

Query: 483 TLESGHYVTYLRLRNRWYKCDDAWITEVDEEMVRNSQCYMMFYLQKTASHKSNEDLSCLP 542
           TLESGHYVTYLRLRN+WYKCDDAWITEVDEE+VR SQCYMMFY+QK   HK++E++S L 
Sbjct: 484 TLESGHYVTYLRLRNQWYKCDDAWITEVDEEIVRASQCYMMFYVQKMLYHKADENMSHLT 543

Query: 543 ISPRNDAFQQIAG 551
            S + +A  ++AG
Sbjct: 544 TSSKEEALHRVAG 556