Cucsa.271340.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.271340.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProtein TIC 55, chloroplastic
Locationscaffold02500 : 253154 .. 255513 (+)
Sequence length1831
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCTCACACAAGTGCTTCGCTATCCTTAAACCAACGTTCATTCCTCTCTTCACCTTCCCTCCAAGACCCTAATTCCTCTCCCATGGCTTCGCTCCGCCCATTTCTTTCCCTTTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTCTCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGAGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTCTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGTAAAATAGGCGGAAAAAATAATGGGCCGTGAAGGTTTCGAACCTGCGATCCGTTATTtGCTACGATATCATTTGATCTTTTAATTTTACCTTCTTATTTTCTCTCTCTTATTTTTtAATTCATCAAATACATCTTATTATATGTAATCTAATATCACTCATACAATTAAGCTAATCTGTGACACTTAGATGGTCTTAATTTAAAATGATTAAAATTTTGAATTATAGCTTAAAATTCAATGTAACTCTCTCCTATTTACATGGTGTGGTTTTAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGGTAATAGAGTCCGACCATGAGTACGTTTGGAATAACTTTTTAACTGTTGAAGAATAGGAGACAAATTCGATTCCTATATTTTCAAATTTATAATCATTTAGTTAACTTATTTTATAATAAATAATAAGTTGTAATATAATAAAAATGACAATTTTGATGAGATATTTTCACTCTTTTATGTAAAGTAGTTTTTTCTATAATTATTTGTATATATTCCTATAAAAAGCTGTATTAGTTGTCTTAATGCAATGCAGCTTCCATCAGATGCCAAGATTCCTAGATCAGCATGTCTAAGAACATACGAAGTAAGGGATTCACAGGGAGTTGTATGGGTATGGATGTCTTTGGAAAAGCCTTCAACCCTAAAAAGTATACCATGGTTTGAGAACTTCGATCGGCCTGGCTTTCGAGATTTTTCGTCCATTCATGAGCTTCCTTATGATCACTCCATACTTCTCGAAAATCTTATGGACCCTGCACATATACCTATCTCCCATGACAGAACGAGTTATCCTGCCAAAAGGGAAGATGCTCAGCCGCTAGACTTCGAGGTTACCGAGCGTACAAATCGCGGTTTTGCCGGTCGGTGGGGCAATGCTAAAGATCAGCCGTTGTCAAATTTCTTGAGATTTGAAGCACCTTGTGTTCTTCAGAATAACAGGGAAACTCCCAATAAGAAAGGTGGGATTGATTACTTTACAGGGCTTTTCCTCTGTAGACCTACAGGCCAAGGGAAATCTATGGTTATAGTGAGATTTGGTAGCACCGCATCAACTGTTAGGTTAAACTTGTTCCCTGCATGGTTTTTGCATCAAAATGCCTGCAGAGTTTTTGAGCAAGACATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAAAGAAAAAGTACCTACCAAGGAACTCTATCTCAACTTACGATCCTCTGACACTTGGGTTCTCGAGTACCGACGTTGGATGGACAAAGTCGGCCATGGGATGCCGTATTACTTTGGACACAGTACGATATCGTTGCCGAAACTGCCTGCTGTTATCGAACATGCCCCCGCGGGAATAGTTGCTGGAACTGCAGCATCTCCTCCGGCTAAGGGGGGAATCGGAACTATGCATGCTCCAAACTTGTCTAATCGATATTTTCGACATATCATTCATTGCAGAAGCTGCAGCAATGTCATTAAATCGTTTCAAACTTGGAATAAGGCTCTTTCTGCCATTGCTATAACATTGATTGCATTGGCAATTTTGGTGAATGGAAGGCAATGGAAGGCTCTCAGTTTGTTATCGGCAGCATTATTCTTTGGTGGAGCTTATGCTTGCTCAGCTGCTTTGACTTTAAATACAGATAATTTTATAAGAACTCATAGAAGGTTGTAAGTTATTGTTTAGATTACAAAATAGTTGGTTGTGGATAGTAAAAAAAaCGTAAATCTACGTTGTTTGTTGATTTCAAAGAAGAGTGTGATTGACGAAAAGAAAACTCTTTCTAAATGATT

mRNA sequence

AATCTCACACAAGTGCTTCGCTATCCTTAAACCAACGTTCATTCCTCTCTTCACCTTCCCTCCAAGACCCTAATTCCTCTCCCATGGCTTCGCTCCGCCCATTTCTTTCCCTTTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTCTCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGAGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTCTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGCTTCCATCAGATGCCAAGATTCCTAGATCAGCATGTCTAAGAACATACGAAGTAAGGGATTCACAGGGAGTTGTATGGGTATGGATGTCTTTGGAAAAGCCTTCAACCCTAAAAAGTATACCATGGTTTGAGAACTTCGATCGGCCTGGCTTTCGAGATTTTTCGTCCATTCATGAGCTTCCTTATGATCACTCCATACTTCTCGAAAATCTTATGGACCCTGCACATATACCTATCTCCCATGACAGAACGAGTTATCCTGCCAAAAGGGAAGATGCTCAGCCGCTAGACTTCGAGGTTACCGAGCGTACAAATCGCGGTTTTGCCGGTCGGTGGGGCAATGCTAAAGATCAGCCGTTGTCAAATTTCTTGAGATTTGAAGCACCTTGTGTTCTTCAGAATAACAGGGAAACTCCCAATAAGAAAGGTGGGATTGATTACTTTACAGGGCTTTTCCTCTGTAGACCTACAGGCCAAGGGAAATCTATGGTTATAGTGAGATTTGGTAGCACCGCATCAACTGTTAGGTTAAACTTGTTCCCTGCATGGTTTTTGCATCAAAATGCCTGCAGAGTTTTTGAGCAAGACATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAAAGAAAAAGTACCTACCAAGGAACTCTATCTCAACTTACGATCCTCTGACACTTGGGTTCTCGAGTACCGACGTTGGATGGACAAAGTCGGCCATGGGATGCCGTATTACTTTGGACACAGTACGATATCGTTGCCGAAACTGCCTGCTGTTATCGAACATGCCCCCGCGGGAATAGTTGCTGGAACTGCAGCATCTCCTCCGGCTAAGGGGGGAATCGGAACTATGCATGCTCCAAACTTGTCTAATCGATATTTTCGACATATCATTCATTGCAGAAGCTGCAGCAATGTCATTAAATCGTTTCAAACTTGGAATAAGGCTCTTTCTGCCATTGCTATAACATTGATTGCATTGGCAATTTTGGTGAATGGAAGGCAATGGAAGGCTCTCAGTTTGTTATCGGCAGCATTATTCTTTGGTGGAGCTTATGCTTGCTCAGCTGCTTTGACTTTAAATACAGATAATTTTATAAGAACTCATAGAAGGTTGTAAGTTATTGTTTAGATTACAAAATAGTTGGTTGTGGATAGTAAAAAAAACGTAAATCTACGTTGTTTGTTGATTTCAAAGAAGAGTGTGATTGACGAAAAGAAAACTCTTTCTAAATGATT

Coding sequence (CDS)

ATGGCTTCGCTCCGCCCATTTCTTTCCCTTTCCCCTGTCTCCCCCGCGCCAAACCCCACACGCCCCACAAATTTCCATCTTCTTCACTCTCCATTATTCTCTTCCTCCTTCAAATTAGTCGCCACTAAAACAAGATTCGCGGTGCGCACTGCCGCCGTCGCCGGCGCCCAAATTGCAGATCCGGTCGATGATAATCAGGTGGTTCTTTTGGGTCCGACAACCAGCGAGAAGCCTGACGATGAAGCTACGGCGGAGTACAATTGGACTGAAGAGTGGTATCCGTTGTATTTGACGAAGGATGTGCCGGAAGATGCTCCGTTAGGGCTCACTGTGTTCGATAAGCAATTAGTTCTGTACCGCGATGGAGCCGGCGAGCTTCACTGTCATGAAGATAGGTGCCCACACAGGCTAGCAAAGCTCTCGGAAGGCCAATTAATCGATGGCAGACTGGAATGTCTTTACCATGGCTGGCAATTTGAAGGTGAGGGGAAATGTGTGAAGATTCCTCAGCTTCCATCAGATGCCAAGATTCCTAGATCAGCATGTCTAAGAACATACGAAGTAAGGGATTCACAGGGAGTTGTATGGGTATGGATGTCTTTGGAAAAGCCTTCAACCCTAAAAAGTATACCATGGTTTGAGAACTTCGATCGGCCTGGCTTTCGAGATTTTTCGTCCATTCATGAGCTTCCTTATGATCACTCCATACTTCTCGAAAATCTTATGGACCCTGCACATATACCTATCTCCCATGACAGAACGAGTTATCCTGCCAAAAGGGAAGATGCTCAGCCGCTAGACTTCGAGGTTACCGAGCGTACAAATCGCGGTTTTGCCGGTCGGTGGGGCAATGCTAAAGATCAGCCGTTGTCAAATTTCTTGAGATTTGAAGCACCTTGTGTTCTTCAGAATAACAGGGAAACTCCCAATAAGAAAGGTGGGATTGATTACTTTACAGGGCTTTTCCTCTGTAGACCTACAGGCCAAGGGAAATCTATGGTTATAGTGAGATTTGGTAGCACCGCATCAACTGTTAGGTTAAACTTGTTCCCTGCATGGTTTTTGCATCAAAATGCCTGCAGAGTTTTTGAGCAAGACATGGGATTTCTTTCATCCCAAAATGAGGTTCTAATGAAAGAAAAAGTACCTACCAAGGAACTCTATCTCAACTTACGATCCTCTGACACTTGGGTTCTCGAGTACCGACGTTGGATGGACAAAGTCGGCCATGGGATGCCGTATTACTTTGGACACAGTACGATATCGTTGCCGAAACTGCCTGCTGTTATCGAACATGCCCCCGCGGGAATAGTTGCTGGAACTGCAGCATCTCCTCCGGCTAAGGGGGGAATCGGAACTATGCATGCTCCAAACTTGTCTAATCGATATTTTCGACATATCATTCATTGCAGAAGCTGCAGCAATGTCATTAAATCGTTTCAAACTTGGAATAAGGCTCTTTCTGCCATTGCTATAACATTGATTGCATTGGCAATTTTGGTGAATGGAAGGCAATGGAAGGCTCTCAGTTTGTTATCGGCAGCATTATTCTTTGGTGGAGCTTATGCTTGCTCAGCTGCTTTGACTTTAAATACAGATAATTTTATAAGAACTCATAGAAGGTTGTAA

Protein sequence

MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL*
BLAST of Cucsa.271340.1 vs. Swiss-Prot
Match: TIC55_PEA (Protein TIC 55, chloroplastic OS=Pisum sativum GN=TIC55 PE=1 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 6.6e-221
Identity = 364/530 (68.68%), Postives = 430/530 (81.13%), Query Frame = 1

Query: 16  APNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVD--DNQVVLLGPT 75
           +P P++ T      S  FS +  L + + + A   AA A  + A  +D  ++Q VL+GP+
Sbjct: 24  SPPPSKKTLLCTNPSSNFSFNKALSSRRRKQAWCVAAAADVKDATLLDGEEDQKVLVGPS 83

Query: 76  TS-EKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGELHCHEDR 135
           +  E+  +   A+Y+WTEEWYPLYLTK+VP DAPLGL V+DK +VL+RDG  +  C+EDR
Sbjct: 84  SEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLGLKVYDKNIVLFRDGNDQFQCYEDR 143

Query: 136 CPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLRTYEVRDSQ 195
           CPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP+DAKIP+SAC++TYEVRDSQ
Sbjct: 144 CPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPADAKIPKSACVKTYEVRDSQ 203

Query: 196 GVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDPAHIPISHD 255
           GV+WVWMS + P  +  IPWFENF RPGF+D S+ HELPYDHSILLENLMDPAH+PISHD
Sbjct: 204 GVLWVWMSRKTPPNVSKIPWFENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHD 263

Query: 256 RTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQNNRETPNKK 315
           RT + AKREDAQ L FEVTERT+RGFAG WG  KD    NFLRFEAPCVLQNNRE  +K 
Sbjct: 264 RTDWSAKREDAQALGFEVTERTDRGFAGWWGREKDGSKPNFLRFEAPCVLQNNREIVDKN 323

Query: 316 GGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFEQDMGFLSS 375
           G I++F+GLFLCRPTGQGKSM+IVRFG+T  +  + LFP W+ HQNA +VFEQDMGFLSS
Sbjct: 324 GEINHFSGLFLCRPTGQGKSMLIVRFGATKRSPLIKLFPEWYFHQNASKVFEQDMGFLSS 383

Query: 376 QNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLPKLPAVIEH 435
           QNE+L+KEKVPTKELYLNL+SSDTWV EYR+WMDKVGHGMPY+FGHSTISLP+ PAV+EH
Sbjct: 384 QNEILLKEKVPTKELYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPEEPAVVEH 443

Query: 436 APAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWNKALSAIAI 495
           APAG+VAG +AS PAKGGIGTMHAPNL+NRYFRH+IHC+ CS+ IK+FQ W   LS + +
Sbjct: 444 APAGLVAGLSASSPAKGGIGTMHAPNLANRYFRHVIHCKGCSSAIKAFQIWKNVLSGVVV 503

Query: 496 TLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL 543
            L ALAILV+GRQWK L L SA+L   G YACS A+ +NT NFIR HRRL
Sbjct: 504 ALAALAILVSGRQWKVLLLASASLCSVGVYACSTAIAMNTTNFIRVHRRL 553

BLAST of Cucsa.271340.1 vs. Swiss-Prot
Match: TIC55_ARATH (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana GN=TIC55 PE=1 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.5e-220
Identity = 375/543 (69.06%), Postives = 435/543 (80.11%), Query Frame = 1

Query: 6   PFLS----LSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAV-RTAAVAGAQIAD 65
           PFLS    L+P SP    T+ T   ++H+     S   + TK R  + R +AVAG  ++D
Sbjct: 4   PFLSSSLQLTPTSPILF-TKVTPTPIIHN---HRSTCTIPTKPRLRLLRRSAVAGTAVSD 63

Query: 66  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 125
             +    VLL P   ++ +    A+Y+WTEEWYPLYLTK+VPEDAPLGLTV+D+Q+VLY+
Sbjct: 64  QTEGGGDVLLNPEEEKRVE---VADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVLYK 123

Query: 126 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 185
           DG G L C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP+ AKIP++
Sbjct: 124 DGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIPKA 183

Query: 186 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 245
           AC++TYEV+DSQGVVWVWMS + P   + +PWFENF RPGF D S+ HELPYDHSILLEN
Sbjct: 184 ACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILLEN 243

Query: 246 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPL-SNFLRFEAP 305
           LMDPAH+PISHDRT + AKREDAQPL FEVTER+NRGFAG WG  K+    SN LRF+AP
Sbjct: 244 LMDPAHVPISHDRTDFTAKREDAQPLVFEVTERSNRGFAGTWGREKEGGKGSNLLRFDAP 303

Query: 306 CVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNA 365
           CVLQNNRE   K G  +YF+GLFLCRPTGQGKSM+IVRFG T  +  +++ P WF HQNA
Sbjct: 304 CVLQNNREFEGKDGVKNYFSGLFLCRPTGQGKSMLIVRFGVTKRSPLVSVLPQWFWHQNA 363

Query: 366 CRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHS 425
           C+VFEQDMGFLSSQNEVLMKEKVPTK+LYLNL+SSDTWV EYR+WMDKVGHGMPY+FGH 
Sbjct: 364 CKVFEQDMGFLSSQNEVLMKEKVPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHR 423

Query: 426 TISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKS 485
           TISLPK+P V+EHAPAG++A  +AS PAKGGIGTMHAPNL+NRYFRHIIHCRSCSNVIKS
Sbjct: 424 TISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVIKS 483

Query: 486 FQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTH 543
           F+ W   LSA A+ L ALAILV  RQWKA+ L SAAL    AY C  A+ LNT+NFIRTH
Sbjct: 484 FELWKNILSATAVALTALAILVVSRQWKAVLLGSAALCSAAAYTCLRAINLNTNNFIRTH 539

BLAST of Cucsa.271340.1 vs. Swiss-Prot
Match: PAO_ARATH (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.6e-41
Identity = 145/544 (26.65%), Postives = 235/544 (43.20%), Query Frame = 1

Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PFLS +   P      P+   L H+PL                R AA      +D  ++ 
Sbjct: 22  PFLSPTTKFPLKVSISPSRSKLFHNPL----------------RVAAPPSVPTSDSTEEK 81

Query: 66  QVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGE 125
           ++            +E  +E+ W + WYP+ L +D+  + P    +  + LVL+ D   +
Sbjct: 82  RI----EEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRDLVLWFDRNDQ 141

Query: 126 LHC-HEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPSD------AKI 185
                +D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ  +        K 
Sbjct: 142 KWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPEARAVKS 201

Query: 186 PRSACLRTYEVRDSQGVVWVW---MSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDH 245
           PR AC   +    SQG+++VW      ++ ++++     ++FD+P F   +   +L Y +
Sbjct: 202 PR-ACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFSTVTIQRDLFYGY 261

Query: 246 SILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFL 305
             L+EN+ DP+HI  +H + +   +R+ A+PL F+V      GF G    A D       
Sbjct: 262 DTLMENVSDPSHIDFAHHKVT--GRRDRAKPLPFKVESSGPWGFQG----ANDDSPRITA 321

Query: 306 RFEAPCVLQNNRETPNKKGGI-DYFTGLFLCR---PTGQGKSMVIVRFG------STAST 365
           +F APC   N  E   K   + +    +++C    P   GK+  IV         S    
Sbjct: 322 KFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGP 381

Query: 366 VRLNLFPAWFLHQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLR---------SSD 425
               + P W+ H  +  V++ DM  L  Q +V + + + + +  +N +          +D
Sbjct: 382 AWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFTPTQAD 441

Query: 426 TWVLEYRRWMDKVGHGMPYYFGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMH 485
            +VL +R W+ + G   P +FG ST S   LP+ +                       + 
Sbjct: 442 RFVLAFRNWLRRHGKSQPEWFG-STPSNQPLPSTV-----------------------LT 501

Query: 486 APNLSNRYFRHIIHCRSCSNVIKSFQTWNKALSAIAITLIALAILVNGRQWK----ALSL 516
              + +R+ +H   C SC     SFQ   K L    +   A A + +  Q +     LSL
Sbjct: 502 KRQMLDRFDQHTQVCSSCKGAYNSFQILKKFLVGATVFWAATAGVPSDVQIRLVLAGLSL 514

BLAST of Cucsa.271340.1 vs. Swiss-Prot
Match: PTC52_ARATH (Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidopsis thaliana GN=PTC52 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 7.2e-26
Identity = 66/194 (34.02%), Postives = 98/194 (50.52%), Query Frame = 1

Query: 73  TTSEKPDDEATAE-----YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRD-GAGEL 132
           +T+  P+ EA  E     ++W   WYP+    D+ +  P G  V    LV++ D    + 
Sbjct: 61  STNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQW 120

Query: 133 HCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPSD---AKIPRSAC 192
              +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ P D       + AC
Sbjct: 121 KVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQAC 180

Query: 193 LRTYEVRDSQGVVWVWMSLEKPSTLKSI-----PWFENFDRPGFRDFSSIHELPYDHSIL 252
           +  Y       ++W W + + P     I     P+    + P F       ++PY + +L
Sbjct: 181 VAVYPSTVQHEIIWFWPNSD-PKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVL 240

BLAST of Cucsa.271340.1 vs. Swiss-Prot
Match: CAO_ORYSJ (Chlorophyllide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica GN=CAO PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 8.8e-24
Identity = 87/332 (26.20%), Postives = 147/332 (44.28%), Query Frame = 1

Query: 92  WYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLE 151
           WYP+  + D+ +D  + +  F++Q V++R   G   C  + C HR   L  G + +GR++
Sbjct: 220 WYPVAFSSDLKDDTMVPIDCFEEQWVIFRGKDGRPGCVMNTCAHRACPLHLGSVNEGRIQ 279

Query: 152 CLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKP-STLKSI 211
           C YHGW++  +GKC K+P          +  +R+    + +G+VW+W   + P ST+ S+
Sbjct: 280 CPYHGWEYSTDGKCEKMPSTKM-----LNVRIRSLPCFEQEGMVWIWPGNDPPKSTIPSL 339

Query: 212 PWFENFDRPGFRDFSSI-HELPYDHSILLENLMDPAHIPISHDRTSYPAKREDAQPLDFE 271
                    GF   + I  ELP +H +LL+NL+D AH P +H  TS  AK      L   
Sbjct: 340 -----LPPSGFTIHAEIVMELPVEHGLLLDNLLDLAHAPFTH--TSTFAKGWSVPSLVKF 399

Query: 272 VTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVL--------QNNRETPNKKGGIDYFTGL 331
           +T   + G  G W      P    + F  PC++            E  + K    +   L
Sbjct: 400 LT--PSSGLQGYW-----DPYPIDMEFRPPCMVLSTIGISKPGKLEGKSTKQCSTHLHQL 459

Query: 332 FLCRPTGQGKSMVIVRFG-STASTVRLNLFPAWFLHQNACRVFEQDMGFLSSQNEVLMKE 391
            +C P+ + K+ ++ R     A  ++   F        A +V  +D+  +  Q E     
Sbjct: 460 HICLPSSRNKTRLLYRMSLDFAPWIKHVPFMHILWSHFAEKVLNEDLRLVLGQQE----R 519

Query: 392 KVPTKELYLNLRSSDTWVLEYRRWMDKVGHGM 413
            +    ++    S D   + YR W D +  G+
Sbjct: 520 MINGANVWNWPVSYDKLGIRYRLWRDAIERGV 528

BLAST of Cucsa.271340.1 vs. TrEMBL
Match: A0A0A0L0A9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651770 PE=4 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 541/542 (99.82%), Postives = 541/542 (99.82%), Query Frame = 1

Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120
           PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR
Sbjct: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120

Query: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 180
           DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS
Sbjct: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 180

Query: 181 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 240
           ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN
Sbjct: 181 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 240

Query: 241 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC 300
           LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC
Sbjct: 241 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC 300

Query: 301 VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC 360
           VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC
Sbjct: 301 VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC 360

Query: 361 RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST 420
           RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST
Sbjct: 361 RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST 420

Query: 421 ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF 480
           ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF
Sbjct: 421 ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF 480

Query: 481 QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHR 540
           QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAAL LNTDNFIRTHR
Sbjct: 481 QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALALNTDNFIRTHR 540

Query: 541 RL 543
           RL
Sbjct: 541 RL 542

BLAST of Cucsa.271340.1 vs. TrEMBL
Match: B9IL92_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02890g PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.6e-228
Identity = 378/538 (70.26%), Postives = 439/538 (81.60%), Query Frame = 1

Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PF S +P +  P  T   N HLL S + S  + +   K   AV  A  A     D  DD 
Sbjct: 17  PFASFTPTTKLPT-TIHINSHLLKS-IHSKPYYI---KKCHAVADAPTAIPPSLDAEDDA 76

Query: 66  QVVLLGPTTSE-KPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAG 125
             VL+GP++ E +  D A  +YNWTEEWYPLYLTKDVP+DAPLGL VFDKQLVLY+DG G
Sbjct: 77  HGVLIGPSSEEERRGDGAVVDYNWTEEWYPLYLTKDVPDDAPLGLNVFDKQLVLYKDGQG 136

Query: 126 ELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLR 185
           EL CHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP++AKIP+SAC++
Sbjct: 137 ELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKIPQSACVK 196

Query: 186 TYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDP 245
           TYEV++SQGVVWVWMSL+ P   K +PWFENF RPGF+D S+IHELPYDHSILLENLMDP
Sbjct: 197 TYEVKESQGVVWVWMSLKTPPNFKKVPWFENFARPGFQDTSTIHELPYDHSILLENLMDP 256

Query: 246 AHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQN 305
           AHIPISHDRT + +KREDAQ L FEVTERT+RGFAGRWG  KDQ L +FLRF+APCVL N
Sbjct: 257 AHIPISHDRTDWTSKREDAQALRFEVTERTHRGFAGRWGKEKDQKLRSFLRFQAPCVLSN 316

Query: 306 NRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFE 365
           + E  ++KG   YFTGLFLCRPTGQGKSM+IVRFG+T       + P W+ HQNAC++FE
Sbjct: 317 DIEFADEKGVTQYFTGLFLCRPTGQGKSMIIVRFGATKRPQMAKVIPKWYFHQNACKIFE 376

Query: 366 QDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLP 425
           QDMGFLSSQNEVLMKEKVPTKELY+NL+SSDTWV EYR+WMDKVGHGMPYYFGH+TISLP
Sbjct: 377 QDMGFLSSQNEVLMKEKVPTKELYINLKSSDTWVAEYRKWMDKVGHGMPYYFGHNTISLP 436

Query: 426 KLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWN 485
           ++PAV+EHAPAG+VAG +AS PAKGGIGTMHAPN++NRYFRH+IHC+ C++V+K+FQ W 
Sbjct: 437 EVPAVVEHAPAGLVAGVSASSPAKGGIGTMHAPNVNNRYFRHVIHCKGCTSVVKNFQAWK 496

Query: 486 KALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL 543
            ALSAI++ L ALAIL +GRQWK   L+SA+L   G YACS A+ +NT NFIRTHRRL
Sbjct: 497 NALSAISVALTALAILASGRQWKTFLLVSASLCLAGVYACSTAIAMNTTNFIRTHRRL 549

BLAST of Cucsa.271340.1 vs. TrEMBL
Match: W9RZ66_9ROSA (Protein TIC 55 OS=Morus notabilis GN=L484_007141 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 4.0e-225
Identity = 373/528 (70.64%), Postives = 431/528 (81.63%), Query Frame = 1

Query: 22  PTNFHLL------HSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDNQVVLLGPTTS 81
           PT  +LL      H  +     K+ A   R +  T   A A +    DD+  V +GPT  
Sbjct: 15  PTTKYLLSLTSHSHPLILFKPLKIPAAPKRLS-HTKCSAVADLKARTDDDNKVFVGPTGE 74

Query: 82  E-KPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGELHCHEDRCP 141
           E +  ++  AEY+WTEEWYPLYLTK++P DAPLGLTVFDKQLVLYRDGAGEL CHEDRCP
Sbjct: 75  EDRRREKVVAEYDWTEEWYPLYLTKNLPHDAPLGLTVFDKQLVLYRDGAGELRCHEDRCP 134

Query: 142 HRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLRTYEVRDSQGV 201
           HRLAKLSEGQ+IDGRLECLYHGWQFEG+GKCVKIPQLP+DAKIPR+ACL+ YEVRDSQGV
Sbjct: 135 HRLAKLSEGQIIDGRLECLYHGWQFEGDGKCVKIPQLPADAKIPRAACLKPYEVRDSQGV 194

Query: 202 VWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDPAHIPISHDRT 261
           +WVWMS + P     +PWFENF RPGF D S+IHELPYDHSILLENLMDPAH+PISHDRT
Sbjct: 195 IWVWMSQKAPPNPSKLPWFENFARPGFNDISTIHELPYDHSILLENLMDPAHVPISHDRT 254

Query: 262 SYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQNNRETPNKKGG 321
            + AKREDAQPL F+V+ER++RGFAG WG + +  L N+LRF+APCVLQNNRE  +K G 
Sbjct: 255 DWTAKREDAQPLAFKVSERSDRGFAGWWGRSSEPELLNYLRFDAPCVLQNNREIVDKNGE 314

Query: 322 IDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFEQDMGFLSSQN 381
             YFTGLFLCRP+GQGKSM+IVRFG+T  +  + LFP W+ HQNAC+VFEQDMGFLSSQN
Sbjct: 315 KHYFTGLFLCRPSGQGKSMLIVRFGNTRRSPLIKLFPEWYFHQNACKVFEQDMGFLSSQN 374

Query: 382 EVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLPKLPAVIEHAP 441
           EVLM+EKVPTKELYLNLRSSDTWV EYRRWMDKVGHGMPY+FGHSTISLPK PAV+EHAP
Sbjct: 375 EVLMREKVPTKELYLNLRSSDTWVAEYRRWMDKVGHGMPYHFGHSTISLPKEPAVVEHAP 434

Query: 442 AGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWNKALSAIAITL 501
           AG+VA  +AS PAKGGIGT+HAPNL+NRYFRH+IHC+ C  V+K+FQ W   LSA+A+ L
Sbjct: 435 AGVVANVSASSPAKGGIGTVHAPNLANRYFRHVIHCKGCREVVKAFQAWKNGLSAVAVVL 494

Query: 502 IALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL 543
            ALAIL +GRQWKAL L++AAL  GGAYAC +A+ LNT NFIRTHRRL
Sbjct: 495 AALAILASGRQWKALLLVTAALCSGGAYACHSAVVLNTTNFIRTHRRL 541

BLAST of Cucsa.271340.1 vs. TrEMBL
Match: V7ATL7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G086000g PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.8e-225
Identity = 378/546 (69.23%), Postives = 446/546 (81.68%), Query Frame = 1

Query: 1   MASLRPFLSLS----PVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGA 60
           MA + PFLS +    P+  +  P +   +  L+SP    S +   +K      TA +  A
Sbjct: 40  MALVNPFLSPTRTFLPLRVSAPPKKVLRW-ALYSPSLKPSLR---SKHASCGATADIKAA 99

Query: 61  QIADPVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQL 120
            +AD  +D + VL+GPT SEK   E   EY+WTEEWYPLYLT++VPEDAPLGLTVFDKQL
Sbjct: 100 PLADSEED-RAVLVGPT-SEK---EGKGEYDWTEEWYPLYLTQNVPEDAPLGLTVFDKQL 159

Query: 121 VLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAK 180
           VL++DG  +  C+EDRCPHRLAKLSEGQL DGRLECLYHGWQFEGEGKCVKIPQLP+DAK
Sbjct: 160 VLFKDGNSQFRCYEDRCPHRLAKLSEGQLFDGRLECLYHGWQFEGEGKCVKIPQLPADAK 219

Query: 181 IPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSI 240
           IPR+AC++TYEVRDSQGV+WVWMSL+ P     +PWFENF RPGFRD S+ HELPYDHSI
Sbjct: 220 IPRTACVKTYEVRDSQGVIWVWMSLKTPPNDSKLPWFENFARPGFRDVSTTHELPYDHSI 279

Query: 241 LLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRF 300
           LLENLMDPAH+PISHDRT + AKRE+AQPL FEVTERT+RGFAG WG  K+    NFLRF
Sbjct: 280 LLENLMDPAHVPISHDRTDWTAKRENAQPLGFEVTERTDRGFAGWWGTEKEGSKGNFLRF 339

Query: 301 EAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLH 360
           EAPCVLQNN+E  +K G  +YF+GLFLCRPTGQGKSM+IVRFGST S+  + +FP W+ H
Sbjct: 340 EAPCVLQNNKEFVDKNGETNYFSGLFLCRPTGQGKSMLIVRFGSTKSSPLVKVFPKWYFH 399

Query: 361 QNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYF 420
           QNAC+VFEQDMGFLSSQNE+L+KEKVPTK+LYLNL+SSDTWV EYR+WMDKVGHGMPYYF
Sbjct: 400 QNACKVFEQDMGFLSSQNEILLKEKVPTKQLYLNLKSSDTWVAEYRKWMDKVGHGMPYYF 459

Query: 421 GHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNV 480
           GHSTISLPK PAV+EHAPAG+VAG +AS PAKG IGT+HAPNL+NRYFRH+IHC+ C  V
Sbjct: 460 GHSTISLPKEPAVVEHAPAGLVAGQSASSPAKGVIGTLHAPNLANRYFRHVIHCKGCRTV 519

Query: 481 IKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFI 540
           +K+FQ W  ALSA+A+TL+ALAILV+G+QWKA+ L SA+L   G YACS A+T+NT NF+
Sbjct: 520 VKAFQAWQNALSAVAVTLVALAILVSGKQWKAILLASASLCSIGVYACSTAITMNTTNFV 576

Query: 541 RTHRRL 543
           RTHRRL
Sbjct: 580 RTHRRL 576

BLAST of Cucsa.271340.1 vs. TrEMBL
Match: A0A067KEQ0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07059 PE=4 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.0e-224
Identity = 378/545 (69.36%), Postives = 438/545 (80.37%), Query Frame = 1

Query: 6   PFLSLS-----PVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFA--VRTAAVAGAQI 65
           PFLS +     P+S +P  T PT   +    L  S  K   TK      ++++ + G+  
Sbjct: 7   PFLSHATSLSKPLSFSPRLTPPTALQIKTHILKPSLIKPYPTKCHATTDLQSSILPGSGR 66

Query: 66  ADPVDDNQVVLLGPTTSEKPDDE-ATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLV 125
            +  +D+ +VL+GP++ E+   E   A+Y+WTEEWYPLYL KDVP+DAPLGLTVFDKQ+V
Sbjct: 67  GE--EDDHMVLVGPSSEEERRGERVVADYHWTEEWYPLYLEKDVPDDAPLGLTVFDKQIV 126

Query: 126 LYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKI 185
           LY+DG GEL CHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP++AKI
Sbjct: 127 LYKDGEGELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKI 186

Query: 186 PRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSIL 245
           PRSAC++TYEVR+SQGVVWVWMS + P  L  +PWFENF RPGF+D S++HELPYDHSIL
Sbjct: 187 PRSACVKTYEVRESQGVVWVWMSQKTPPNLNKLPWFENFARPGFQDTSTVHELPYDHSIL 246

Query: 246 LENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFE 305
           LENLMDPAHIPISHDRT + AKREDAQPL FEVTERT+RGFAGRWG  KD  L NFLRFE
Sbjct: 247 LENLMDPAHIPISHDRTDWTAKREDAQPLCFEVTERTDRGFAGRWGKEKDNGLPNFLRFE 306

Query: 306 APCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQ 365
           APCVLQNNRE  ++ G   YFTGLFLCRPTGQGKSM+IVRFG T  +      P W+ HQ
Sbjct: 307 APCVLQNNREIIDENGEKQYFTGLFLCRPTGQGKSMLIVRFGGTKRSYIAKWIPNWYFHQ 366

Query: 366 NACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFG 425
           NA +VFEQDMGFLSSQNEVLMKEKVPTK LYLNLRSSDTWV EYR+WMDKVGHGMPYYFG
Sbjct: 367 NASKVFEQDMGFLSSQNEVLMKEKVPTKNLYLNLRSSDTWVAEYRKWMDKVGHGMPYYFG 426

Query: 426 HSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVI 485
           HSTISLP++PAV+EHAPAG+VAG +AS PAKGGIGTMHAPN +NRYFRH+IHC+ CS+V+
Sbjct: 427 HSTISLPQVPAVVEHAPAGLVAGVSASLPAKGGIGTMHAPNFANRYFRHVIHCKECSSVV 486

Query: 486 KSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIR 543
           K+F  W   LS IA+ L ALAIL +GRQWK+  L+SA+L   G Y CS A+ +NT NFIR
Sbjct: 487 KAFDAWKNGLSGIALILTALAILASGRQWKSFFLVSASLCLAGVYGCSTAIAMNTTNFIR 546

BLAST of Cucsa.271340.1 vs. TAIR10
Match: AT2G24820.1 (AT2G24820.1 translocon at the inner envelope membrane of chloroplasts 55-II)

HSP 1 Score: 766.1 bits (1977), Expect = 1.4e-221
Identity = 375/543 (69.06%), Postives = 435/543 (80.11%), Query Frame = 1

Query: 6   PFLS----LSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAV-RTAAVAGAQIAD 65
           PFLS    L+P SP    T+ T   ++H+     S   + TK R  + R +AVAG  ++D
Sbjct: 4   PFLSSSLQLTPTSPILF-TKVTPTPIIHN---HRSTCTIPTKPRLRLLRRSAVAGTAVSD 63

Query: 66  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 125
             +    VLL P   ++ +    A+Y+WTEEWYPLYLTK+VPEDAPLGLTV+D+Q+VLY+
Sbjct: 64  QTEGGGDVLLNPEEEKRVE---VADYDWTEEWYPLYLTKNVPEDAPLGLTVYDRQIVLYK 123

Query: 126 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 185
           DG G L C+EDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP+ AKIP++
Sbjct: 124 DGEGTLRCYEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPASAKIPKA 183

Query: 186 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 245
           AC++TYEV+DSQGVVWVWMS + P   + +PWFENF RPGF D S+ HELPYDHSILLEN
Sbjct: 184 ACVKTYEVKDSQGVVWVWMSTKTPPNPEKLPWFENFARPGFFDISTTHELPYDHSILLEN 243

Query: 246 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPL-SNFLRFEAP 305
           LMDPAH+PISHDRT + AKREDAQPL FEVTER+NRGFAG WG  K+    SN LRF+AP
Sbjct: 244 LMDPAHVPISHDRTDFTAKREDAQPLVFEVTERSNRGFAGTWGREKEGGKGSNLLRFDAP 303

Query: 306 CVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNA 365
           CVLQNNRE   K G  +YF+GLFLCRPTGQGKSM+IVRFG T  +  +++ P WF HQNA
Sbjct: 304 CVLQNNREFEGKDGVKNYFSGLFLCRPTGQGKSMLIVRFGVTKRSPLVSVLPQWFWHQNA 363

Query: 366 CRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHS 425
           C+VFEQDMGFLSSQNEVLMKEKVPTK+LYLNL+SSDTWV EYR+WMDKVGHGMPY+FGH 
Sbjct: 364 CKVFEQDMGFLSSQNEVLMKEKVPTKDLYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHR 423

Query: 426 TISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKS 485
           TISLPK+P V+EHAPAG++A  +AS PAKGGIGTMHAPNL+NRYFRHIIHCRSCSNVIKS
Sbjct: 424 TISLPKVPPVVEHAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVIKS 483

Query: 486 FQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTH 543
           F+ W   LSA A+ L ALAILV  RQWKA+ L SAAL    AY C  A+ LNT+NFIRTH
Sbjct: 484 FELWKNILSATAVALTALAILVVSRQWKAVLLGSAALCSAAAYTCLRAINLNTNNFIRTH 539

BLAST of Cucsa.271340.1 vs. TAIR10
Match: AT3G44880.1 (AT3G44880.1 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain)

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-42
Identity = 145/544 (26.65%), Postives = 235/544 (43.20%), Query Frame = 1

Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PFLS +   P      P+   L H+PL                R AA      +D  ++ 
Sbjct: 22  PFLSPTTKFPLKVSISPSRSKLFHNPL----------------RVAAPPSVPTSDSTEEK 81

Query: 66  QVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGE 125
           ++            +E  +E+ W + WYP+ L +D+  + P    +  + LVL+ D   +
Sbjct: 82  RI----EEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRDLVLWFDRNDQ 141

Query: 126 LHC-HEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPSD------AKI 185
                +D CPHRLA LSEG+L + G L+C YHGW F G G C +IPQ  +        K 
Sbjct: 142 KWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPEARAVKS 201

Query: 186 PRSACLRTYEVRDSQGVVWVW---MSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDH 245
           PR AC   +    SQG+++VW      ++ ++++     ++FD+P F   +   +L Y +
Sbjct: 202 PR-ACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFSTVTIQRDLFYGY 261

Query: 246 SILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFL 305
             L+EN+ DP+HI  +H + +   +R+ A+PL F+V      GF G    A D       
Sbjct: 262 DTLMENVSDPSHIDFAHHKVT--GRRDRAKPLPFKVESSGPWGFQG----ANDDSPRITA 321

Query: 306 RFEAPCVLQNNRETPNKKGGI-DYFTGLFLCR---PTGQGKSMVIVRFG------STAST 365
           +F APC   N  E   K   + +    +++C    P   GK+  IV         S    
Sbjct: 322 KFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGP 381

Query: 366 VRLNLFPAWFLHQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLR---------SSD 425
               + P W+ H  +  V++ DM  L  Q +V + + + + +  +N +          +D
Sbjct: 382 AWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFTPTQAD 441

Query: 426 TWVLEYRRWMDKVGHGMPYYFGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMH 485
            +VL +R W+ + G   P +FG ST S   LP+ +                       + 
Sbjct: 442 RFVLAFRNWLRRHGKSQPEWFG-STPSNQPLPSTV-----------------------LT 501

Query: 486 APNLSNRYFRHIIHCRSCSNVIKSFQTWNKALSAIAITLIALAILVNGRQWK----ALSL 516
              + +R+ +H   C SC     SFQ   K L    +   A A + +  Q +     LSL
Sbjct: 502 KRQMLDRFDQHTQVCSSCKGAYNSFQILKKFLVGATVFWAATAGVPSDVQIRLVLAGLSL 514

BLAST of Cucsa.271340.1 vs. TAIR10
Match: AT4G25650.2 (AT4G25650.2 ACD1-like)

HSP 1 Score: 120.2 bits (300), Expect = 4.0e-27
Identity = 66/194 (34.02%), Postives = 98/194 (50.52%), Query Frame = 1

Query: 73  TTSEKPDDEATAE-----YNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRD-GAGEL 132
           +T+  P+ EA  E     ++W   WYP+    D+ +  P G  V    LV++ D    + 
Sbjct: 61  STNSPPEPEALFEPGSDKFDWYANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQW 120

Query: 133 HCHEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCVKIPQLPSD---AKIPRSAC 192
              +D CPHRLA LS+G++   GRL+C+YHGW F G G C  IPQ P D       + AC
Sbjct: 121 KVMDDTCPHRLAPLSDGRIDQWGRLQCVYHGWCFNGSGDCKLIPQAPPDGPPVHTFKQAC 180

Query: 193 LRTYEVRDSQGVVWVWMSLEKPSTLKSI-----PWFENFDRPGFRDFSSIHELPYDHSIL 252
           +  Y       ++W W + + P     I     P+    + P F       ++PY + +L
Sbjct: 181 VAVYPSTVQHEIIWFWPNSD-PKYKNIIETNKPPYIPELEDPSFTKLMGNRDIPYGYDVL 240

BLAST of Cucsa.271340.1 vs. TAIR10
Match: AT1G44446.1 (AT1G44446.1 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain)

HSP 1 Score: 113.2 bits (282), Expect = 4.9e-25
Identity = 87/332 (26.20%), Postives = 148/332 (44.58%), Query Frame = 1

Query: 92  WYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLE 151
           WYP+  T D+  D  + +  F++  V++R   G+  C  + C HR   L  G + +GR++
Sbjct: 221 WYPVAFTADLKHDTMVPIECFEQPWVIFRGEDGKPGCVRNTCAHRACPLDLGTVNEGRIQ 280

Query: 152 CLYHGWQFEGEGKCVKIPQLP-SDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSI 211
           C YHGW++  +G+C K+P       KI    CL      + +G++W+W   E P+     
Sbjct: 281 CPYHGWEYSTDGECKKMPSTKLLKVKIKSLPCL------EQEGMIWIWPGDEPPA----- 340

Query: 212 PWFENFDRP-GFRDFSS-IHELPYDHSILLENLMDPAHIPISHDRTSYPAKREDAQPLDF 271
           P   +   P GF   +  + +LP +H +LL+NL+D AH P +H  TS  AK      L  
Sbjct: 341 PILPSLQPPSGFLIHAELVMDLPVEHGLLLDNLLDLAHAPFTH--TSTFAKGWSVPSLVK 400

Query: 272 EVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQN--NRETPNKKGG------IDYFTG 331
            +T  +  G  G W      P    + F+ PC++ +      P K  G        +   
Sbjct: 401 FLTPTS--GLQGYW-----DPYPIDMEFKPPCIVLSTIGISKPGKLEGKSTQQCATHLHQ 460

Query: 332 LFLCRPTGQGKSMVIVRFGSTASTVRLNL-FPAWFLHQNACRVFEQDMGFLSSQNEVLMK 391
           L +C P+ + K+ ++ R     + +  NL F        A +V  +D+  +  Q E ++ 
Sbjct: 461 LHVCLPSSKNKTRLLYRMSLDFAPILKNLPFMEHLWRHFAEQVLNEDLRLVLGQQERMLN 520

Query: 392 EKVPTKELYLNLRSSDTWVLEYRRWMDKVGHG 412
                  ++    + D   + YR W + V  G
Sbjct: 521 ----GANIWNLPVAYDKLGVRYRLWRNAVDRG 528

BLAST of Cucsa.271340.1 vs. NCBI nr
Match: gi|449455677|ref|XP_004145578.1| (PREDICTED: protein TIC 55, chloroplastic [Cucumis sativus])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 541/542 (99.82%), Postives = 541/542 (99.82%), Query Frame = 1

Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD
Sbjct: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60

Query: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120
           PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR
Sbjct: 61  PVDDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYR 120

Query: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 180
           DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS
Sbjct: 121 DGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRS 180

Query: 181 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 240
           ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN
Sbjct: 181 ACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLEN 240

Query: 241 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC 300
           LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC
Sbjct: 241 LMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPC 300

Query: 301 VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC 360
           VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC
Sbjct: 301 VLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNAC 360

Query: 361 RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST 420
           RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST
Sbjct: 361 RVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHST 420

Query: 421 ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF 480
           ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF
Sbjct: 421 ISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSF 480

Query: 481 QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHR 540
           QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAAL LNTDNFIRTHR
Sbjct: 481 QTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALALNTDNFIRTHR 540

Query: 541 RL 543
           RL
Sbjct: 541 RL 542

BLAST of Cucsa.271340.1 vs. NCBI nr
Match: gi|659104126|ref|XP_008452818.1| (PREDICTED: protein TIC 55, chloroplastic [Cucumis melo])

HSP 1 Score: 1060.1 bits (2740), Expect = 1.3e-306
Identity = 516/547 (94.33%), Postives = 529/547 (96.71%), Query Frame = 1

Query: 1   MASLRPFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIAD 60
           MASLRPFLSLSPV PAPN TRPT+  L+H+P FSSSFKLV+ K RFAVRTAAVAGA+IAD
Sbjct: 92  MASLRPFLSLSPVFPAPNHTRPTDSLLIHAP-FSSSFKLVSAKPRFAVRTAAVAGAEIAD 151

Query: 61  PV-----DDNQVVLLGPTTSEKPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQ 120
           PV     DDNQVVLL PTTSEKPD EATAEYNWTEEWYPLYLT+DVPEDAPLGLTVFDKQ
Sbjct: 152 PVGDLKDDDNQVVLLDPTTSEKPDGEATAEYNWTEEWYPLYLTRDVPEDAPLGLTVFDKQ 211

Query: 121 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDA 180
           LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDA
Sbjct: 212 LVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDA 271

Query: 181 KIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHS 240
           KIPRSACLRTYEVR+SQGVVWVWMSLEKPSTLK+IPWFENFDRPGFRDFSSIHELPYDHS
Sbjct: 272 KIPRSACLRTYEVRESQGVVWVWMSLEKPSTLKNIPWFENFDRPGFRDFSSIHELPYDHS 331

Query: 241 ILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLR 300
           ILLENLMDPAHIPISHDRTSYPAKREDAQPL FEVTERTNRGFAGRWGNAK+QPLSNFLR
Sbjct: 332 ILLENLMDPAHIPISHDRTSYPAKREDAQPLAFEVTERTNRGFAGRWGNAKEQPLSNFLR 391

Query: 301 FEAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFL 360
           FEAPCVLQNNRET NKKG IDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFP+WFL
Sbjct: 392 FEAPCVLQNNRETTNKKGEIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPSWFL 451

Query: 361 HQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYY 420
           HQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYY
Sbjct: 452 HQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYY 511

Query: 421 FGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSN 480
           FGHSTISLPKLPAVIEHAPAG+VAGTAASPPAKGGIGTMHAPNL+NRYFRHIIHCRSCSN
Sbjct: 512 FGHSTISLPKLPAVIEHAPAGLVAGTAASPPAKGGIGTMHAPNLNNRYFRHIIHCRSCSN 571

Query: 481 VIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNF 540
           VIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAA+ LNTDNF
Sbjct: 572 VIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAAVALNTDNF 631

Query: 541 IRTHRRL 543
           IRTHRRL
Sbjct: 632 IRTHRRL 637

BLAST of Cucsa.271340.1 vs. NCBI nr
Match: gi|1009134110|ref|XP_015884267.1| (PREDICTED: protein TIC 55, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 814.7 bits (2103), Expect = 9.7e-233
Identity = 396/552 (71.74%), Postives = 452/552 (81.88%), Query Frame = 1

Query: 1   MASL-RPFLS--------LSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTA 60
           MA+L  PFLS        LSP S  P+PT    F +  +PL      L + K +   +  
Sbjct: 1   MAALSHPFLSRILLSSSLLSPTSKPPSPTTKFLFSISSNPLKP----LPSRKNQ--TQCG 60

Query: 61  AVAGAQIADPVDDNQVVLLGPTTSEKPDDEA-TAEYNWTEEWYPLYLTKDVPEDAPLGLT 120
           A+A  + + P DD++ VLL  T  E+   E   A+Y+WTEEWYPLYLT+DVPEDAPLGLT
Sbjct: 61  AIADIKASPPFDDHKDVLLDATIEEEHRGERDVADYDWTEEWYPLYLTRDVPEDAPLGLT 120

Query: 121 VFDKQLVLYRDGAGELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQ 180
           VFDKQLVLYRDGAGEL C++DRCPHRLAKLSEGQLIDG+LECLYHGWQF+G+GKCVKIPQ
Sbjct: 121 VFDKQLVLYRDGAGELRCYQDRCPHRLAKLSEGQLIDGKLECLYHGWQFQGDGKCVKIPQ 180

Query: 181 LPSDAKIPRSACLRTYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHEL 240
           LP+DAKIPRSACL+TYEVRDSQGVVWVWMS + P     +PWFENF RPGF+D S+IHEL
Sbjct: 181 LPADAKIPRSACLKTYEVRDSQGVVWVWMSQKTPPNPSKLPWFENFARPGFQDISTIHEL 240

Query: 241 PYDHSILLENLMDPAHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPL 300
           PYDHSILLENLMDPAHIPISHDRT + AKREDAQ L FEVTER+NRGFAG WG A DQPL
Sbjct: 241 PYDHSILLENLMDPAHIPISHDRTDWTAKREDAQALGFEVTERSNRGFAGWWGKATDQPL 300

Query: 301 SNFLRFEAPCVLQNNRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLF 360
            NFLRFEAPCVLQNNRE  +K G  DYFTGLFLCRP+GQGKSM+IVRFG T  +  + LF
Sbjct: 301 PNFLRFEAPCVLQNNREIVDKNGQKDYFTGLFLCRPSGQGKSMLIVRFGGTKRSPLIKLF 360

Query: 361 PAWFLHQNACRVFEQDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGH 420
           P W+ HQNAC+VFEQDMGFLSSQNEVL+KEKVPTKELYLNLRSSDTWV EYR+WMDKVGH
Sbjct: 361 PQWYFHQNACKVFEQDMGFLSSQNEVLIKEKVPTKELYLNLRSSDTWVAEYRKWMDKVGH 420

Query: 421 GMPYYFGHSTISLPKLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHC 480
           GMPY+FGHSTI LPK PAV+EHAPAG+VAG +AS PAKGGIG+MH PNL+NRYFRH+IHC
Sbjct: 421 GMPYHFGHSTILLPKEPAVVEHAPAGLVAGVSASAPAKGGIGSMHVPNLNNRYFRHVIHC 480

Query: 481 RSCSNVIKSFQTWNKALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTL 540
           + C N +K+FQTW  ALSAIAI ++A+AILV+GRQWKAL L++AA   GGAYACSAA+ L
Sbjct: 481 KGCRNAVKAFQTWKIALSAIAIAMVAVAILVSGRQWKALLLVAAAACSGGAYACSAAIAL 540

Query: 541 NTDNFIRTHRRL 543
           NT NFIR HRR+
Sbjct: 541 NTTNFIRIHRRV 546

BLAST of Cucsa.271340.1 vs. NCBI nr
Match: gi|743882349|ref|XP_011036725.1| (PREDICTED: protein TIC 55, chloroplastic-like [Populus euphratica])

HSP 1 Score: 799.7 bits (2064), Expect = 3.2e-228
Identity = 379/538 (70.45%), Postives = 440/538 (81.78%), Query Frame = 1

Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PF S SP +  P  T   N HLL S + S  + +   +   AV  A  A     D  DD 
Sbjct: 17  PFASFSPTTKLPT-TIHINSHLLKS-IHSKPYHI---QKCHAVADAPTAIPPSLDAEDDA 76

Query: 66  QVVLLGPTTSE-KPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAG 125
             VL+GP++ E + +D A  +YNWTEEWYPLYLTKDVP+DAPLGL VFDKQLVLY+DG G
Sbjct: 77  HGVLIGPSSEEERREDGAVVDYNWTEEWYPLYLTKDVPDDAPLGLNVFDKQLVLYKDGQG 136

Query: 126 ELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLR 185
           EL CHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP++AKIP+SAC++
Sbjct: 137 ELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKIPQSACVK 196

Query: 186 TYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDP 245
           TYEV++SQGVVWVWMSL+ P   K +PWFENF RPGF+D S+IHELPYDHSILLENLMDP
Sbjct: 197 TYEVKESQGVVWVWMSLKTPPNFKKVPWFENFARPGFQDTSTIHELPYDHSILLENLMDP 256

Query: 246 AHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQN 305
           AHIPISHDRT + AKREDAQ L FEVTERT+RGFAGRWG  KDQ L +FLRF+APCVL N
Sbjct: 257 AHIPISHDRTDWTAKREDAQALRFEVTERTHRGFAGRWGKEKDQKLLSFLRFQAPCVLSN 316

Query: 306 NRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFE 365
           + E  ++KG   YFTGLFLCRPTGQGKSM+IVRFG+T       + P W+ HQNAC++FE
Sbjct: 317 DIEFADEKGVTQYFTGLFLCRPTGQGKSMIIVRFGATKRPQMAKVIPKWYFHQNACKIFE 376

Query: 366 QDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLP 425
           QDMGFLSSQNEVLMKEKVPTKELY+NL+SSDTWV EYR+WMDKVGHGMPYYFGH+TISLP
Sbjct: 377 QDMGFLSSQNEVLMKEKVPTKELYINLKSSDTWVAEYRKWMDKVGHGMPYYFGHNTISLP 436

Query: 426 KLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWN 485
           ++PAV+EHAPAG+VAG +AS PAKGGIGTMHAPN++NRYFRH+IHC+ C++V+K+FQ W 
Sbjct: 437 EVPAVVEHAPAGLVAGVSASSPAKGGIGTMHAPNVNNRYFRHVIHCKGCTSVVKNFQAWK 496

Query: 486 KALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL 543
            ALSAI++ L ALAIL +GRQWK   L+SA+L   G YACS A+ +NT NFIRTHRRL
Sbjct: 497 NALSAISVALTALAILASGRQWKTFLLVSASLCLAGVYACSTAIAMNTTNFIRTHRRL 549

BLAST of Cucsa.271340.1 vs. NCBI nr
Match: gi|224143358|ref|XP_002324928.1| (hypothetical protein POPTR_0018s02890g [Populus trichocarpa])

HSP 1 Score: 797.7 bits (2059), Expect = 1.2e-227
Identity = 378/538 (70.26%), Postives = 439/538 (81.60%), Query Frame = 1

Query: 6   PFLSLSPVSPAPNPTRPTNFHLLHSPLFSSSFKLVATKTRFAVRTAAVAGAQIADPVDDN 65
           PF S +P +  P  T   N HLL S + S  + +   K   AV  A  A     D  DD 
Sbjct: 17  PFASFTPTTKLPT-TIHINSHLLKS-IHSKPYYI---KKCHAVADAPTAIPPSLDAEDDA 76

Query: 66  QVVLLGPTTSE-KPDDEATAEYNWTEEWYPLYLTKDVPEDAPLGLTVFDKQLVLYRDGAG 125
             VL+GP++ E +  D A  +YNWTEEWYPLYLTKDVP+DAPLGL VFDKQLVLY+DG G
Sbjct: 77  HGVLIGPSSEEERRGDGAVVDYNWTEEWYPLYLTKDVPDDAPLGLNVFDKQLVLYKDGQG 136

Query: 126 ELHCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPSDAKIPRSACLR 185
           EL CHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLP++AKIP+SAC++
Sbjct: 137 ELRCHEDRCPHRLAKLSEGQLIDGRLECLYHGWQFEGEGKCVKIPQLPANAKIPQSACVK 196

Query: 186 TYEVRDSQGVVWVWMSLEKPSTLKSIPWFENFDRPGFRDFSSIHELPYDHSILLENLMDP 245
           TYEV++SQGVVWVWMSL+ P   K +PWFENF RPGF+D S+IHELPYDHSILLENLMDP
Sbjct: 197 TYEVKESQGVVWVWMSLKTPPNFKKVPWFENFARPGFQDTSTIHELPYDHSILLENLMDP 256

Query: 246 AHIPISHDRTSYPAKREDAQPLDFEVTERTNRGFAGRWGNAKDQPLSNFLRFEAPCVLQN 305
           AHIPISHDRT + +KREDAQ L FEVTERT+RGFAGRWG  KDQ L +FLRF+APCVL N
Sbjct: 257 AHIPISHDRTDWTSKREDAQALRFEVTERTHRGFAGRWGKEKDQKLRSFLRFQAPCVLSN 316

Query: 306 NRETPNKKGGIDYFTGLFLCRPTGQGKSMVIVRFGSTASTVRLNLFPAWFLHQNACRVFE 365
           + E  ++KG   YFTGLFLCRPTGQGKSM+IVRFG+T       + P W+ HQNAC++FE
Sbjct: 317 DIEFADEKGVTQYFTGLFLCRPTGQGKSMIIVRFGATKRPQMAKVIPKWYFHQNACKIFE 376

Query: 366 QDMGFLSSQNEVLMKEKVPTKELYLNLRSSDTWVLEYRRWMDKVGHGMPYYFGHSTISLP 425
           QDMGFLSSQNEVLMKEKVPTKELY+NL+SSDTWV EYR+WMDKVGHGMPYYFGH+TISLP
Sbjct: 377 QDMGFLSSQNEVLMKEKVPTKELYINLKSSDTWVAEYRKWMDKVGHGMPYYFGHNTISLP 436

Query: 426 KLPAVIEHAPAGIVAGTAASPPAKGGIGTMHAPNLSNRYFRHIIHCRSCSNVIKSFQTWN 485
           ++PAV+EHAPAG+VAG +AS PAKGGIGTMHAPN++NRYFRH+IHC+ C++V+K+FQ W 
Sbjct: 437 EVPAVVEHAPAGLVAGVSASSPAKGGIGTMHAPNVNNRYFRHVIHCKGCTSVVKNFQAWK 496

Query: 486 KALSAIAITLIALAILVNGRQWKALSLLSAALFFGGAYACSAALTLNTDNFIRTHRRL 543
            ALSAI++ L ALAIL +GRQWK   L+SA+L   G YACS A+ +NT NFIRTHRRL
Sbjct: 497 NALSAISVALTALAILASGRQWKTFLLVSASLCLAGVYACSTAIAMNTTNFIRTHRRL 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TIC55_PEA6.6e-22168.68Protein TIC 55, chloroplastic OS=Pisum sativum GN=TIC55 PE=1 SV=1[more]
TIC55_ARATH2.5e-22069.06Protein TIC 55, chloroplastic OS=Arabidopsis thaliana GN=TIC55 PE=1 SV=1[more]
PAO_ARATH4.6e-4126.65Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1[more]
PTC52_ARATH7.2e-2634.02Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidop... [more]
CAO_ORYSJ8.8e-2426.20Chlorophyllide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica GN=CAO... [more]
Match NameE-valueIdentityDescription
A0A0A0L0A9_CUCSA0.0e+0099.82Uncharacterized protein OS=Cucumis sativus GN=Csa_4G651770 PE=4 SV=1[more]
B9IL92_POPTR8.6e-22870.26Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0018s02890g PE=4 SV=1[more]
W9RZ66_9ROSA4.0e-22570.64Protein TIC 55 OS=Morus notabilis GN=L484_007141 PE=4 SV=1[more]
V7ATL7_PHAVU6.8e-22569.23Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G086000g PE=4 SV=1[more]
A0A067KEQ0_JATCU2.0e-22469.36Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07059 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G24820.11.4e-22169.06 translocon at the inner envelope membrane of chloroplasts 55-II[more]
AT3G44880.12.6e-4226.65 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain[more]
AT4G25650.24.0e-2734.02 ACD1-like[more]
AT1G44446.14.9e-2526.20 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain[more]
Match NameE-valueIdentityDescription
gi|449455677|ref|XP_004145578.1|0.0e+0099.82PREDICTED: protein TIC 55, chloroplastic [Cucumis sativus][more]
gi|659104126|ref|XP_008452818.1|1.3e-30694.33PREDICTED: protein TIC 55, chloroplastic [Cucumis melo][more]
gi|1009134110|ref|XP_015884267.1|9.7e-23371.74PREDICTED: protein TIC 55, chloroplastic [Ziziphus jujuba][more]
gi|743882349|ref|XP_011036725.1|3.2e-22870.45PREDICTED: protein TIC 55, chloroplastic-like [Populus euphratica][more]
gi|224143358|ref|XP_002324928.1|1.2e-22770.26hypothetical protein POPTR_0018s02890g [Populus trichocarpa][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR013626PaO
IPR017941Rieske_2Fe-2S
Vocabulary: Molecular Function
TermDefinition
GO:0010277chlorophyllide a oxygenase [overall] activity
GO:00515372 iron, 2 sulfur cluster binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0045036 protein targeting to chloroplast
cellular_component GO:0009941 chloroplast envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0010277 chlorophyllide a oxygenase [overall] activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.271340Cucsa.271340gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.271340.1Cucsa.271340.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.271340.1.five_prime_UTR.1Cucsa.271340.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.271340.1.CDS.1Cucsa.271340.1.CDS.1CDS
Cucsa.271340.1.CDS.2Cucsa.271340.1.CDS.2CDS
Cucsa.271340.1.CDS.3Cucsa.271340.1.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.271340.1.three_prime_UTR.1Cucsa.271340.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013626Pheophorbide a oxygenasePFAMPF08417PaOcoord: 296..381
score: 1.0
IPR017941Rieske [2Fe-2S] iron-sulphur domainGENE3DG3DSA:2.102.10.10coord: 90..205
score: 7.0
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 91..173
score: 4.0
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROFILEPS51296RIESKEcoord: 92..197
score: 33
IPR017941Rieske [2Fe-2S] iron-sulphur domainunknownSSF50022ISP domaincoord: 82..202
score: 1.03
NoneNo IPR availablePANTHERPTHR21266IRON-SULFUR DOMAIN CONTAINING PROTEINcoord: 451..542
score: 0.0coord: 1..425
score:
NoneNo IPR availablePANTHERPTHR21266:SF29PROTEIN TIC 55, CHLOROPLASTICcoord: 1..425
score: 0.0coord: 451..542
score:
NoneNo IPR availableunknownSSF55961Bet v1-likecoord: 193..411
score: 2.75