Cucsa.259440.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.259440.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionKinase, putative
Locationscaffold02229 : 5934638 .. 5937958 (+)
Sequence length1963
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGAAGTATGGAGTCTCCTGATAATGCTGCTGAAAAAGATCCTACCGGTCGATACGTTCGGGTATAATACTGAATGCTGGGATTTATTTTAATTGCTCTGCTTCATTCTATATTGAAATTTTAGATAACTGAGTTTTTTtATTCATGGGTAACAATTATTTTTtCTGTATTTTTCAATTTTGAACAACTTTGATGCACGTGGCTATGTTCTTGAGATCTGTTTTATTGATTCATTATTCCACATCCGGCTATATTAAGTTATCTCAAGATTCAATCGTCTCTAGGTCATCCCTGTTAGAATATTTGTTTCCCCAATTAAAAGAAAaCAATACAATATATGCCTTGGAATGCAAGTTTTCACTTCAGGACAAAAGAAACACAACCCAGACTTGTATTTTCTTTTGAATAGTTTAGTTTGTGTTTTAGACCATTTACTTCCATTGACAGTGAGGATAAGTTGTGTGGTGTCTCTTTCTGGAAAGTTTATTCAACGAGGTCGGGAAAATGTGGCGTTGGTAGATTAAAAAATGTGCTTGCTCCTAATAACCACTAGACACAAGTTCAAAGTACATTTAACAACTTTCTTCAATACATCCAATGGTGCAAAATTGTCAGCTACGTTGTGGCAGCCTCTTCAAATAATTTCTCCTGCTTCTGGTTTGCACTTTTGTTTCCTTCAGTTATTTATTTAAATTTGTGTATTCATCTCTTTTCCCTCGTCTGTGGACAACAGTACGATGAAATCCTGGGAAGGGGCGCTTTCAAGACAGTGTATGGTTCTTAGACTTGATAACTCTGTTCTCTTACTGCTTTTTAGATGTCTTGTGAAATGTATTTGCTTTAGTAGAGAAAACTATATGAAGGATTCTTTTGGTGGTTTCTAAGAATTTCTATTTTCTTTTCCCCCTGTATTTAGTTACAAGGCATTCGACGAAGTGGATGGAATTGAAGTTGCCTGGAATCAAGTTAGGATTGATGGTTTCTTGCAATCACCTGAGGATTTGGAAAAGTTGTACTCTGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAGTATGTATTCAGTCTTTTTGTTTATATAGTGCTGACTTAATTTTCTGGTTAGTTAAATGCTTGCCTTTTGGCTATCAGATATCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAATTGGGCGCGACAGATTCTCCGAGGGTTGGTTTATCTTCACAGTCATGATCCTCCCATTATTCATAGAGATTTGAAAGGCGATAACATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCTCGTAGTGTAATTGGTACTGCAACAAATTAATGTGTATCACCTCTCGTCTTAATTTAAATAGAAACACAAAATAGGGGAAAAAGTACTGAAATTCTTTTTtCTTTATTCAAGGAACTCCTGAGTTCATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTGACCTTTGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTTTAGGAAAGTTACATCTGTAAGCAAAACAATCATCACTTCATCGTCATGCTACACGTTAACTGTCTTTTGTGACCATAGGTCCATAATATCAATGCTGACCTTCTTCTTAATCAGGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACGGACCATGGAATTCATCAATAAATGTTTGGTTCCAGTGCACGAGAGGTTGTCTGCAAAAGAGCTTCTTAAAGACTCGTTCCTTCAAGTTGAGAACCCAAAGGAATCGGCACGCAATCCCCTACAGCTATCTAATCAGGTTTCTAAATCAATAAATTTGCCCAAGAGTGGACCTATTTCCATGGACATTGACATTGACCAAAAAaTACATTCTTTAAGCACTTATGCTGAAAGCAATAGCGGGAGTCCACGCTTTCCAGTCGTGGAATTCCAGACAATGAATAAGAACAATGAGTTCAGGTTACGTGGAAATAAGAATGACGATAACTCTGTGGCATTAACTCTGCGTATTGCAGACTCAAATGGTAAGCTTAACTTTTAGACTCACCTTTTCCCTTTCTCTACCTGATTGGCTTTTTTtCCTTCCTCATTATCAACGTCAATTACATTTTTAACTTGCTAAAGTTCTCGTCTATGTACTTTGTTGAGAACTCTTTTTGTAGACTTGTTATCTGCATGTTCTTGTGCAATTGTTAGTTTTTTTCAATGAAAGTTGGTTTCTTACCCCCCAAAAAACAATACTTAAACTTGCAGGTCGAGTGAGGAATATACATTTTACGTTTTATCTCGATTCTGATACTGCATTGTCAGTTGCTGCTGAAATGGCTGAACAGTTGGAGTTGATAAATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATAACAAAGCTTATACCTGAATGGAAGCCCTTGTCTGTTTATTCCTCAAATGGAGAATTGAGTCTTTTCAGCGCCCCCCcTTTCCTTAAAAGTGCCAAATCATCTATAGGTTCTGCTTGGGGTTCAATCTTAACTGGGAGTCATGATGGATTGGTCGCACAAGACATTTCTTCTGGGTTGGGGTGTGGTACTCAAAAGGATTGTCTGCAATCTGAAGAAGATGGCTGGACTACTGATATTTCCGCTGGTCACATTTTTGATACTTGCCCTTCTTCACCTAGTTTGGCTAATTTTGAGGACTTGAATTCACATGCTTCATTTGCGTTGGAGTTACTGGTCGACGATTGCTCTACCAAAAGTGCTAAGGTGTTTGATTGTTCCAATATCGATGGAAGTTCAAAAGGCTCGAGCTGGTCGATTGCAGAACTAGAGCACCACGGTTCATCATATGTTGTTGAAGACAAATTTCAAAGAAATGTTGGTGATGTTGGAATCTTCACTCCCATGGATTATTTTGCGAAGAACTCGGTGGTGTCAATGCCTGCTCCAAGTGAAGCATCTAATGTGATGAGCTTGACAAGCAGTTGCTCTTCGTTATCGTTAACAGACAAGGATCTCGATGCTGAACTGAAGATGGAAATCGATGCTATCGAGACACATTATCGGCAACTGTTTGATGAGCTTTCTCGAATGAGAGAGGAGGCACTAGAGGCCACCAGAAGGAGATGGATTGCAAAGAAGAAACTGATTCATTGA

mRNA sequence

TTTGGAAGTATGGAGTCTCCTGATAATGCTGCTGAAAAAGATCCTACCGGTCGATACGTTCGGTTACAAGGCATTCGACGAAGTGGATGGAATTGAAGTTGCCTGGAATCAAGTTAGGATTGATGGTTTCTTGCAATCACCTGAGGATTTGGAAAAGTTGTACTCTGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAATATCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAATTGGGCGCGACAGATTCTCCGAGGGTTGGTTTATCTTCACAGTCATGATCCTCCCATTATTCATAGAGATTTGAAAGGCGATAACATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCTCGTAGTGTAATTGGAACTCCTGAGTTCATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTGACCTTTGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTTTAGGAAAGTTACATCTGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACGGACCATGGAATTCATCAATAAATGTTTGGTTCCAGTGCACGAGAGGTTGTCTGCAAAAGAGCTTCTTAAAGACTCGTTCCTTCAAGTTGAGAACCCAAAGGAATCGGCACGCAATCCCCTACAGCTATCTAATCAGGTTTCTAAATCAATAAATTTGCCCAAGAGTGGACCTATTTCCATGGACATTGACATTGACCAAAAAATACATTCTTTAAGCACTTATGCTGAAAGCAATAGCGGGAGTCCACGCTTTCCAGTCGTGGAATTCCAGACAATGAATAAGAACAATGAGTTCAGGTTACGTGGAAATAAGAATGACGATAACTCTGTGGCATTAACTCTGCGTATTGCAGACTCAAATGGTCGAGTGAGGAATATACATTTTACGTTTTATCTCGATTCTGATACTGCATTGTCAGTTGCTGCTGAAATGGCTGAACAGTTGGAGTTGATAAATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATAACAAAGCTTATACCTGAATGGAAGCCCTTGTCTGTTTATTCCTCAAATGGAGAATTGAGTCTTTTCAGCGCCCCCCCTTTCCTTAAAAGTGCCAAATCATCTATAGGTTCTGCTTGGGGTTCAATCTTAACTGGGAGTCATGATGGATTGGTCGCACAAGACATTTCTTCTGGGTTGGGGTGTGGTACTCAAAAGGATTGTCTGCAATCTGAAGAAGATGGCTGGACTACTGATATTTCCGCTGGTCACATTTTTGATACTTGCCCTTCTTCACCTAGTTTGGCTAATTTTGAGGACTTGAATTCACATGCTTCATTTGCGTTGGAGTTACTGGTCGACGATTGCTCTACCAAAAGTGCTAAGGTGTTTGATTGTTCCAATATCGATGGAAGTTCAAAAGGCTCGAGCTGGTCGATTGCAGAACTAGAGCACCACGGTTCATCATATGTTGTTGAAGACAAATTTCAAAGAAATGTTGGTGATGTTGGAATCTTCACTCCCATGGATTATTTTGCGAAGAACTCGGTGGTGTCAATGCCTGCTCCAAGTGAAGCATCTAATGTGATGAGCTTGACAAGCAGTTGCTCTTCGTTATCGTTAACAGACAAGGATCTCGATGCTGAACTGAAGATGGAAATCGATGCTATCGAGACACATTATCGGCAACTGTTTGATGAGCTTTCTCGAATGAGAGAGGAGGCACTAGAGGCCACCAGAAGGAGATGGATTGCAAAGAAGAAACTGATTCATTGA

Coding sequence (CDS)

ATGCTGCTGAAAAAGATCCTACCGGTCGATACGTTCGGTTACAAGGCATTCGACGAAGTGGATGGAATTGAAGTTGCCTGGAATCAAGTTAGGATTGATGGTTTCTTGCAATCACCTGAGGATTTGGAAAAGTTGTACTCTGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAATATCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAATTGGGCGCGACAGATTCTCCGAGGGTTGGTTTATCTTCACAGTCATGATCCTCCCATTATTCATAGAGATTTGAAAGGCGATAACATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCTCGTAGTGTAATTGGAACTCCTGAGTTCATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTGACCTTTGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTTTAGGAAAGTTACATCTGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACGGACCATGGAATTCATCAATAAATGTTTGGTTCCAGTGCACGAGAGGTTGTCTGCAAAAGAGCTTCTTAAAGACTCGTTCCTTCAAGTTGAGAACCCAAAGGAATCGGCACGCAATCCCCTACAGCTATCTAATCAGGTTTCTAAATCAATAAATTTGCCCAAGAGTGGACCTATTTCCATGGACATTGACATTGACCAAAAAaTACATTCTTTAAGCACTTATGCTGAAAGCAATAGCGGGAGTCCACGCTTTCCAGTCGTGGAATTCCAGACAATGAATAAGAACAATGAGTTCAGGTTACGTGGAAATAAGAATGACGATAACTCTGTGGCATTAACTCTGCGTATTGCAGACTCAAATGGTCGAGTGAGGAATATACATTTTACGTTTTATCTCGATTCTGATACTGCATTGTCAGTTGCTGCTGAAATGGCTGAACAGTTGGAGTTGATAAATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATAACAAAGCTTATACCTGAATGGAAGCCCTTGTCTGTTTATTCCTCAAATGGAGAATTGAGTCTTTTCAGCGCCCCCCcTTTCCTTAAAAGTGCCAAATCATCTATAGGTTCTGCTTGGGGTTCAATCTTAACTGGGAGTCATGATGGATTGGTCGCACAAGACATTTCTTCTGGGTTGGGGTGTGGTACTCAAAAGGATTGTCTGCAATCTGAAGAAGATGGCTGGACTACTGATATTTCCGCTGGTCACATTTTTGATACTTGCCCTTCTTCACCTAGTTTGGCTAATTTTGAGGACTTGAATTCACATGCTTCATTTGCGTTGGAGTTACTGGTCGACGATTGCTCTACCAAAAGTGCTAAGGTGTTTGATTGTTCCAATATCGATGGAAGTTCAAAAGGCTCGAGCTGGTCGATTGCAGAACTAGAGCACCACGGTTCATCATATGTTGTTGAAGACAAATTTCAAAGAAATGTTGGTGATGTTGGAATCTTCACTCCCATGGATTATTTTGCGAAGAACTCGGTGGTGTCAATGCCTGCTCCAAGTGAAGCATCTAATGTGATGAGCTTGACAAGCAGTTGCTCTTCGTTATCGTTAACAGACAAGGATCTCGATGCTGAACTGAAGATGGAAATCGATGCTATCGAGACACATTATCGGCAACTGTTTGATGAGCTTTCTCGAATGAGAGAGGAGGCACTAGAGGCCACCAGAAGGAGATGGATTGCAAAGAAGAAACTGATTCATTGA

Protein sequence

MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLIH*
BLAST of Cucsa.259440.1 vs. Swiss-Prot
Match: WNK4_ORYSJ (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica GN=WNK4 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 5.9e-144
Identity = 256/387 (66.15%), Postives = 308/387 (79.59%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEV+GIEVAW+QV ID  +QSP++LE+LYSEVHLLKSLKHEN++KFYN WVDD+KK
Sbjct: 40  YKAFDEVEGIEVAWSQVEIDEVMQSPDNLERLYSEVHLLKSLKHENVMKFYNYWVDDQKK 99

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           T+N+ITELFTSGSLRQYR+KH  VD+KAIKNWARQ+LRGL YLH+H PPIIHRDLK DNI
Sbjct: 100 TINVITELFTSGSLRQYRQKHPRVDLKAIKNWARQVLRGLDYLHTHQPPIIHRDLKCDNI 159

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGNHGEVKIGDLGLA VM  P A+SVIGTPEFMAPELY+E Y+ELVD+YSFGMCMLEM
Sbjct: 160 FVNGNHGEVKIGDLGLATVMLTPRAKSVIGTPEFMAPELYDENYDELVDIYSFGMCMLEM 219

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
            T EYPYSEC N AQIF+KV+ G+KPA+LAK+++ +  +FI+KCLVP  ERLSAKELL+D
Sbjct: 220 FTLEYPYSECTNAAQIFKKVSKGVKPAALAKITNIQAKQFIDKCLVPASERLSAKELLQD 279

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
            FL  +N   S     +  + + KS+++     + MD+D ++ +   ST   ++ G P  
Sbjct: 280 PFLCSDN--SSVLVGTKFPSSLPKSVDVSLEA-LHMDVDTNESM-CTSTCKRNDLGGPHR 339

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
            V+EF   NKN E +L G K DDNSV+L LRIAD  G  RNIHF FYLDSDTA+SVAAEM
Sbjct: 340 SVLEFTRTNKNTELKLTGEKLDDNSVSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEM 399

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIP 401
            EQLEL + DV FIA+FID LI  L+P
Sbjct: 400 VEQLELADCDVTFIADFIDLLIVNLVP 422


HSP 2 Score: 45.4 bits (106), Expect = 2.7e-03
Identity = 37/120 (30.83%), Postives = 55/120 (45.83%), Query Frame = 1

Query: 523 IDGSSKGSSWSIAELEHHGSSY-VVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEAS 582
           +DGSSK  S      E+    Y  +E    +    +G   P+   + N  +      +AS
Sbjct: 504 LDGSSKSLS------EYGVDEYRTLECGAYKGTDKLGCRHPLSNGSSNFAIFQM--DQAS 563

Query: 583 NVMSLTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKK 642
           +   L    S     ++D+   L  E+  IE  Y Q F EL+RMREEALE  R++W+  K
Sbjct: 564 HHSELVIGASVSITENRDV---LNGELGLIEAQYEQWFRELTRMREEALEGARKKWLPDK 612

BLAST of Cucsa.259440.1 vs. Swiss-Prot
Match: WNK7_ARATH (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana GN=WNK7 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.4e-140
Identity = 257/406 (63.30%), Postives = 307/406 (75.62%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           +K FDEVDGIEVAWNQVRID  LQSP+ LE+LYSEV LLKSLKH+NII+FYNSW+DDK K
Sbjct: 43  FKGFDEVDGIEVAWNQVRIDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNK 102

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVN+ITELFTSGSLRQYRKKH+ V+MKA+K WARQIL GL YLHS DPPIIHRD+K DNI
Sbjct: 103 TVNIITELFTSGSLRQYRKKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNI 162

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEM
Sbjct: 163 FINGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEM 222

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPY EC+N AQI++KV+SGIKPASL+KV DP  M+FI KCL+P  ERLSA+ELL D
Sbjct: 223 VTFEYPYCECRNSAQIYKKVSSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLD 282

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPI----------------SMDIDIDQKI 313
           SFL V     +  NPL L + V     +PK G                  +M +++D+  
Sbjct: 283 SFLNVNGLVMN--NPLPLPDIV-----MPKEGSFGERCLMSEGPPNARNRTMSMNLDEDN 342

Query: 314 HSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHF 373
           +     + +NSG+     +E +   + N F L+G +ND+NSV+L LRI D NGRVRNIHF
Sbjct: 343 NLPIVISSNNSGTN---CIEVRRAKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHF 402

Query: 374 TFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWK 404
            F+ + DTA +V++EM EQLEL + +V FIAE ID L+  LIP WK
Sbjct: 403 LFFQEGDTASNVSSEMVEQLELTDKNVKFIAELIDVLLVNLIPNWK 438


HSP 2 Score: 40.0 bits (92), Expect = 1.1e-01
Identity = 19/48 (39.58%), Postives = 30/48 (62.50%), Query Frame = 1

Query: 594 SLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKK 642
           SL +++ D  LK E++ IE  +R+   E++R REEA   T+ R+  KK
Sbjct: 505 SLEEEEEDERLKEELEKIEERFREEMKEITRKREEATMETKNRFFEKK 552

BLAST of Cucsa.259440.1 vs. Swiss-Prot
Match: WNK6_ARATH (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana GN=WNK6 PE=2 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 3.0e-140
Identity = 259/404 (64.11%), Postives = 304/404 (75.25%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRID  LQSP  LE+LYSEV LLKSLKH NII+FYNSW+DDK K
Sbjct: 43  YKAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNK 102

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVN+ITELFTSGSLR YRKKH+ V+MKA+KNWARQIL GL YLH  +PPIIHRDLK DNI
Sbjct: 103 TVNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNI 162

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEM
Sbjct: 163 FINGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEM 222

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTF+YPY ECKN AQI++KV+SGIKPASL++V DP   +FI KCL+P  ERLSAKELL D
Sbjct: 223 VTFDYPYCECKNSAQIYKKVSSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLD 282

Query: 254 SFLQVENPKESARNPLQLSNQVSKSIN------LPKSGP--------ISMDIDIDQKIHS 313
            FLQ+     +  NPL L + V           L   GP        +S+D+D D  +  
Sbjct: 283 PFLQLNG--LTMNNPLPLPDIVMPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNL-P 342

Query: 314 LSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTF 373
           + T+++ NSGS     +E +   + N F L+G +ND+ SV+L LRI D NGRVRNIHF F
Sbjct: 343 IVTFSD-NSGS---RCIEVRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLF 402

Query: 374 YLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWK 404
           Y + DTA  V++EM EQLEL + +V FIAE ID L+  +IP WK
Sbjct: 403 YQEGDTASKVSSEMVEQLELTDQNVTFIAELIDILLVNMIPTWK 439

BLAST of Cucsa.259440.1 vs. Swiss-Prot
Match: WNK8_ARATH (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana GN=WNK8 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 7.2e-134
Identity = 242/388 (62.37%), Postives = 294/388 (75.77%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWN V I+  +Q P  LE+LYSEVHLLK+LKHENIIK + SWVD+K K
Sbjct: 44  YKAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNK 103

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           T+NMITELFTSGSLR YRKKH+ VD KAIKNWARQIL+GL YLHS +PP+IHRDLK DNI
Sbjct: 104 TINMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNI 163

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGN GEVKIGDLGLA V+QQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEM
Sbjct: 164 FVNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEM 223

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VT EYPY+EC+N AQI++KVTS IKP SL KV DP+  +FI KCL+P   R +A EL KD
Sbjct: 224 VTCEYPYNECRNQAQIYKKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKD 283

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
            FL  +  K+SA   L  S+  SK +  P+   + MD+D     H+ +    SN   P  
Sbjct: 284 PFLARDGGKDSAL--LASSSTSSKYVRPPQLEHLPMDVD-----HNENKSVSSNEDYPWS 343

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
             +E Q + +N EFRLRG ++DD + ++ LRIAD +G+ R +HF FYL+SDTA ++A EM
Sbjct: 344 QTIELQRIAENKEFRLRGERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEM 403

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPE 402
            E+L L + +V  IA+ ID  I +L+ +
Sbjct: 404 VEELHLTSQEVVVIADMIDDFIMQLLSD 424


HSP 2 Score: 59.7 bits (143), Expect = 1.4e-07
Identity = 29/84 (34.52%), Postives = 51/84 (60.71%), Query Frame = 1

Query: 565 YFAKNSVVSMPAPSEASNVMSLTSSCS------SLSLTDKDLDAELKMEIDAIETHYRQL 624
           Y A +   +M A  +A ++ S   SCS      +LS++D D   +LK E++ IE+ + Q 
Sbjct: 474 YSANDGNAAMEAGRDAESMSSYLDSCSMMSTIYNLSISDNDYPEDLKTELNLIESQFNQS 533

Query: 625 FDELSRMREEALEATRRRWIAKKK 643
           F +L +++E+A+E  +R+WI KK+
Sbjct: 534 FQDLLKLKEDAIENAKRKWITKKQ 557

BLAST of Cucsa.259440.1 vs. Swiss-Prot
Match: WNK10_ARATH (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana GN=WNK10 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.9e-126
Identity = 243/444 (54.73%), Postives = 302/444 (68.02%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEV+GIEVAWN + I+  LQ P  L++LYSEVHLL SLKH+NIIK + SWVDD  K
Sbjct: 31  YKAFDEVEGIEVAWNLMSIEDVLQMPGQLDRLYSEVHLLNSLKHDNIIKLFYSWVDDHNK 90

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           ++NMITELFTSGSL  YRKKH+ VD KAI NWARQIL+GL YLHS  PP+IHRDLK DNI
Sbjct: 91  SINMITELFTSGSLTLYRKKHRKVDPKAIMNWARQILKGLHYLHSQTPPVIHRDLKCDNI 150

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGN G+VKIGDLGLA VMQQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEM
Sbjct: 151 FVNGNTGKVKIGDLGLAAVMQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEM 210

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VT EYPY EC+N AQI++KVTSGIKP SL+KV DP+  +FI KCL+P   R +A ELLKD
Sbjct: 211 VTCEYPYRECRNQAQIYKKVTSGIKPQSLSKVDDPQVKQFIEKCLLPAPSRPTALELLKD 270

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIH-SLSTYAESNSGSPR 313
             L V+  K+S       SN   K    P+     MD++  +    S+ + A+S+     
Sbjct: 271 QLLAVDGAKDSTLT--ASSNTTFKPAMPPQCEYRPMDVEYKKNTSVSICSSAKSSQECAL 330

Query: 314 FPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAE 373
              +E Q + ++ EF+L G + DD + ++ LRIA S+G+ R + F F L +DTA +V  E
Sbjct: 331 LQTMEVQRVAESTEFKLSGERRDDVAASMALRIAGSSGQARKVDFDFNLKTDTARAVTGE 390

Query: 374 MAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGS 433
           M E+L+L +H+V  IAE ID LI KL                SL +A    +S     G 
Sbjct: 391 MVEELDLSSHEVTVIAEMIDELIMKL------------KANRSLPNANSVYQSKDEEAGE 450

Query: 434 AWGSILTGSHDGLVAQDISSGLGC 457
           +  S ++  +   V+ +  S LGC
Sbjct: 451 SMKSEISADYYHRVSSNEGSRLGC 460


HSP 2 Score: 43.9 bits (102), Expect = 7.8e-03
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 1

Query: 586 LTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWI 639
           L+S   S S+       +LK E++ IE+ Y Q    L RM+EEA+E  +R+W+
Sbjct: 469 LSSFLDSCSMVSNKQSEDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of Cucsa.259440.1 vs. TrEMBL
Match: A0A0A0L3S7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119370 PE=4 SV=1)

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 632/632 (100.00%), Postives = 632/632 (100.00%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK
Sbjct: 102 YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 161

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI
Sbjct: 162 TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 221

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM
Sbjct: 222 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 281

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD
Sbjct: 282 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 341

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
           SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF
Sbjct: 342 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 401

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
           PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM
Sbjct: 402 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 461

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 433
           AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA
Sbjct: 462 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 521

Query: 434 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 493
           WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE
Sbjct: 522 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 581

Query: 494 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 553
           DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN
Sbjct: 582 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 641

Query: 554 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 613
           VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET
Sbjct: 642 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 701

Query: 614 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 646
           HYRQLFDELSRMREEALEATRRRWIAKKKLIH
Sbjct: 702 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 733

BLAST of Cucsa.259440.1 vs. TrEMBL
Match: A0A067HF81_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.6e-220
Identity = 419/643 (65.16%), Postives = 486/643 (75.58%), Query Frame = 1

Query: 4   KKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKF 63
           ++I  VDT  YKAFDE  GIEVAW++V+ID  LQ P DL+KLYSEVHLLKSLK +N++KF
Sbjct: 8   RRIPAVDT--YKAFDEFAGIEVAWSRVKIDDVLQLPGDLQKLYSEVHLLKSLKDDNVVKF 67

Query: 64  YNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPI 123
           Y SWVDDKKKTVNMITELFTSG+LRQYRKKHK+VD+K IKNWARQIL GLVYLHSH+PPI
Sbjct: 68  YKSWVDDKKKTVNMITELFTSGNLRQYRKKHKNVDIKVIKNWARQILHGLVYLHSHNPPI 127

Query: 124 IHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDV 183
           IHRDLK DNIF+NGNHGEVKIGDLGLAI MQQPTARSVIGTPEFMAPELYEEEYNELVD+
Sbjct: 128 IHRDLKCDNIFVNGNHGEVKIGDLGLAIAMQQPTARSVIGTPEFMAPELYEEEYNELVDI 187

Query: 184 YSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHE 243
           YSFGMC+LEMVTFEYPYSECKNPAQIF+KVTSGIKPASLAKV+DP+   FI KCLVP  E
Sbjct: 188 YSFGMCILEMVTFEYPYSECKNPAQIFKKVTSGIKPASLAKVNDPQIKGFIEKCLVPASE 247

Query: 244 RLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTY 303
           RLSAK+LLKD FLQVEN KE   +PL+L  Q  K + LP SGP SMDID D K  SLST 
Sbjct: 248 RLSAKDLLKDPFLQVENQKEPICDPLKLPIQSLKMLRLPMSGPSSMDIDSDYKQLSLSTC 307

Query: 304 AESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDS 363
            ESN+GSP  PV+EFQ M+KNNEFRLRG KNDD SV+LTLRIADS+GRVRNIHF FYLDS
Sbjct: 308 TESNNGSPHCPVLEFQRMHKNNEFRLRGTKNDDTSVSLTLRIADSSGRVRNIHFLFYLDS 367

Query: 364 DTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFL 423
           DTALSVA EM EQL+L +HDV FIAEFID+LI KL+P WKP   YSS+G LS +S  P L
Sbjct: 368 DTALSVAGEMVEQLDLADHDVAFIAEFIDYLIMKLLPGWKPSYDYSSSGALSFYSVSPIL 427

Query: 424 KSAKSSIGSAWGSILTG-SHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDT 483
            + K+S  S W ++  G   + +V QD+ SG      +D   ++ +    +   G I   
Sbjct: 428 GNGKTSTPSPWDAMGAGVPSEFVVKQDVVSGPTRNPSQDLAPAQGENLHDNAGGGGI--- 487

Query: 484 CPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCS--NIDGSSKGSSWSIAELEHH 543
             SSPSLA  ED  S +S A ++ VDD STK+ K  + S  + D S K  +  ++ELE  
Sbjct: 488 --SSPSLAKLEDQESQSSVASDIFVDDTSTKNDKASEFSDYSTDRSYKDLNGYVSELE-L 547

Query: 544 GSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDL 603
           G     E K Q N  + G    +  F KNS ++ P     S V+SLTSS SSLSLT KD+
Sbjct: 548 GDLCYDECKLQGNYSEDGEDILLYQFVKNSKLTFP---NLSTVLSLTSSYSSLSLTGKDV 607

Query: 604 DAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 644
           D ELKMEIDAIE  Y   F ELS+M+EEALEA+R+RW+AKK+L
Sbjct: 608 D-ELKMEIDAIEAQYEHWFQELSKMKEEALEASRKRWMAKKRL 638

BLAST of Cucsa.259440.1 vs. TrEMBL
Match: V4TWU9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019052mg PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 6.0e-220
Identity = 417/646 (64.55%), Postives = 484/646 (74.92%), Query Frame = 1

Query: 1   MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENI 60
           M+L  +       YKAFDE  GIEVAW++V+ID  LQ P DL+KLYSEVHLLKSLK +N+
Sbjct: 1   MVLTGLYMTPFSSYKAFDEFAGIEVAWSRVKIDDVLQLPGDLQKLYSEVHLLKSLKDDNV 60

Query: 61  IKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHD 120
           +KFY SWVDDKKKTVNMITELFTSG+LRQYRKKHK+VD+K IKNWARQIL GLVYLHSH+
Sbjct: 61  VKFYKSWVDDKKKTVNMITELFTSGNLRQYRKKHKNVDIKVIKNWARQILHGLVYLHSHN 120

Query: 121 PPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNEL 180
           PPIIHRDLK DNIF+NGNHGEVKIGDLGLAI MQQPTARSVIGTPEFMAPELYEEEYNEL
Sbjct: 121 PPIIHRDLKCDNIFVNGNHGEVKIGDLGLAIAMQQPTARSVIGTPEFMAPELYEEEYNEL 180

Query: 181 VDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVP 240
           VD+YSFGMC+LEMVTFEYPYSECKNPAQIF+KVTSGIKPASLAKV+DP+   FI KCLVP
Sbjct: 181 VDIYSFGMCILEMVTFEYPYSECKNPAQIFKKVTSGIKPASLAKVNDPQIKGFIEKCLVP 240

Query: 241 VHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSL 300
             ERLSAK+LLKD FLQVEN KE   +PL+L  Q  K + LP SGP SMDID D K  SL
Sbjct: 241 ASERLSAKDLLKDPFLQVENQKEPICDPLKLPIQSLKMLRLPMSGPSSMDIDSDYKQLSL 300

Query: 301 STYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFY 360
           ST  ESN+GSP  PV+EFQ M+KNNEFRLRG KNDD SV+LTLRIADS+GRVRNIHF FY
Sbjct: 301 STCTESNNGSPHCPVLEFQRMHKNNEFRLRGTKNDDTSVSLTLRIADSSGRVRNIHFLFY 360

Query: 361 LDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAP 420
           LDSDTALSVA EM EQL+L +HDV FIAEFID+LI KL+P WKP   YSS+G LS +S  
Sbjct: 361 LDSDTALSVAGEMVEQLDLADHDVAFIAEFIDYLIMKLLPGWKPSYDYSSSGALSFYSVS 420

Query: 421 PFLKSAKSSIGSAWGSILTG-SHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHI 480
           P L + K+S  S W ++  G   + +V QD+ SG      +D   ++ +    +   G I
Sbjct: 421 PILGNGKTSTPSPWDAMGAGVPSEFVVKQDVVSGPTRNPSQDLAPAQGENLHDNAGGGGI 480

Query: 481 FDTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCS--NIDGSSKGSSWSIAEL 540
                SSPSLA  ED  S +S A ++ VDD STK+ K  + S  + D S K  +  ++EL
Sbjct: 481 -----SSPSLAKLEDQESQSSVASDIFVDDTSTKNDKASEFSDYSTDRSYKDLNGYVSEL 540

Query: 541 EHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTD 600
           E  G     E K Q N  + G    +  F KNS ++ P     S V+SLTSS SSLSLT 
Sbjct: 541 E-LGDLCYDECKLQGNYSEDGEDILLYQFVKNSKLTFP---NLSTVLSLTSSYSSLSLTG 600

Query: 601 KDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 644
           KD+D ELKMEIDAIE  Y   F ELS+M+EEALEA+R+RW+AKK+L
Sbjct: 601 KDVD-ELKMEIDAIEAQYEHWFQELSKMKEEALEASRKRWMAKKRL 636

BLAST of Cucsa.259440.1 vs. TrEMBL
Match: A0A067HEX2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 6.0e-220
Identity = 417/646 (64.55%), Postives = 484/646 (74.92%), Query Frame = 1

Query: 1   MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENI 60
           M+L  +       YKAFDE  GIEVAW++V+ID  LQ P DL+KLYSEVHLLKSLK +N+
Sbjct: 1   MVLTGLYMTPFSSYKAFDEFAGIEVAWSRVKIDDVLQLPGDLQKLYSEVHLLKSLKDDNV 60

Query: 61  IKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHD 120
           +KFY SWVDDKKKTVNMITELFTSG+LRQYRKKHK+VD+K IKNWARQIL GLVYLHSH+
Sbjct: 61  VKFYKSWVDDKKKTVNMITELFTSGNLRQYRKKHKNVDIKVIKNWARQILHGLVYLHSHN 120

Query: 121 PPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNEL 180
           PPIIHRDLK DNIF+NGNHGEVKIGDLGLAI MQQPTARSVIGTPEFMAPELYEEEYNEL
Sbjct: 121 PPIIHRDLKCDNIFVNGNHGEVKIGDLGLAIAMQQPTARSVIGTPEFMAPELYEEEYNEL 180

Query: 181 VDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVP 240
           VD+YSFGMC+LEMVTFEYPYSECKNPAQIF+KVTSGIKPASLAKV+DP+   FI KCLVP
Sbjct: 181 VDIYSFGMCILEMVTFEYPYSECKNPAQIFKKVTSGIKPASLAKVNDPQIKGFIEKCLVP 240

Query: 241 VHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSL 300
             ERLSAK+LLKD FLQVEN KE   +PL+L  Q  K + LP SGP SMDID D K  SL
Sbjct: 241 ASERLSAKDLLKDPFLQVENQKEPICDPLKLPIQSLKMLRLPMSGPSSMDIDSDYKQLSL 300

Query: 301 STYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFY 360
           ST  ESN+GSP  PV+EFQ M+KNNEFRLRG KNDD SV+LTLRIADS+GRVRNIHF FY
Sbjct: 301 STCTESNNGSPHCPVLEFQRMHKNNEFRLRGTKNDDTSVSLTLRIADSSGRVRNIHFLFY 360

Query: 361 LDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAP 420
           LDSDTALSVA EM EQL+L +HDV FIAEFID+LI KL+P WKP   YSS+G LS +S  
Sbjct: 361 LDSDTALSVAGEMVEQLDLADHDVAFIAEFIDYLIMKLLPGWKPSYDYSSSGALSFYSVS 420

Query: 421 PFLKSAKSSIGSAWGSILTG-SHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHI 480
           P L + K+S  S W ++  G   + +V QD+ SG      +D   ++ +    +   G I
Sbjct: 421 PILGNGKTSTPSPWDAMGAGVPSEFVVKQDVVSGPTRNPSQDLAPAQGENLHDNAGGGGI 480

Query: 481 FDTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCS--NIDGSSKGSSWSIAEL 540
                SSPSLA  ED  S +S A ++ VDD STK+ K  + S  + D S K  +  ++EL
Sbjct: 481 -----SSPSLAKLEDQESQSSVASDIFVDDTSTKNDKASEFSDYSTDRSYKDLNGYVSEL 540

Query: 541 EHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTD 600
           E  G     E K Q N  + G    +  F KNS ++ P     S V+SLTSS SSLSLT 
Sbjct: 541 E-LGDLCYDECKLQGNYSEDGEDILLYQFVKNSKLTFP---NLSTVLSLTSSYSSLSLTG 600

Query: 601 KDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 644
           KD+D ELKMEIDAIE  Y   F ELS+M+EEALEA+R+RW+AKK+L
Sbjct: 601 KDVD-ELKMEIDAIEAQYEHWFQELSKMKEEALEASRKRWMAKKRL 636

BLAST of Cucsa.259440.1 vs. TrEMBL
Match: A0A067H6I0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 7.8e-220
Identity = 415/633 (65.56%), Postives = 480/633 (75.83%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDE  GIEVAW++V+ID  LQ P DL+KLYSEVHLLKSLK +N++KFY SWVDDKKK
Sbjct: 94  YKAFDEFAGIEVAWSRVKIDDVLQLPGDLQKLYSEVHLLKSLKDDNVVKFYKSWVDDKKK 153

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVNMITELFTSG+LRQYRKKHK+VD+K IKNWARQIL GLVYLHSH+PPIIHRDLK DNI
Sbjct: 154 TVNMITELFTSGNLRQYRKKHKNVDIKVIKNWARQILHGLVYLHSHNPPIIHRDLKCDNI 213

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGNHGEVKIGDLGLAI MQQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMC+LEM
Sbjct: 214 FVNGNHGEVKIGDLGLAIAMQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCILEM 273

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPYSECKNPAQIF+KVTSGIKPASLAKV+DP+   FI KCLVP  ERLSAK+LLKD
Sbjct: 274 VTFEYPYSECKNPAQIFKKVTSGIKPASLAKVNDPQIKGFIEKCLVPASERLSAKDLLKD 333

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
            FLQVEN KE   +PL+L  Q  K + LP SGP SMDID D K  SLST  ESN+GSP  
Sbjct: 334 PFLQVENQKEPICDPLKLPIQSLKMLRLPMSGPSSMDIDSDYKQLSLSTCTESNNGSPHC 393

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
           PV+EFQ M+KNNEFRLRG KNDD SV+LTLRIADS+GRVRNIHF FYLDSDTALSVA EM
Sbjct: 394 PVLEFQRMHKNNEFRLRGTKNDDTSVSLTLRIADSSGRVRNIHFLFYLDSDTALSVAGEM 453

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 433
            EQL+L +HDV FIAEFID+LI KL+P WKP   YSS+G LS +S  P L + K+S  S 
Sbjct: 454 VEQLDLADHDVAFIAEFIDYLIMKLLPGWKPSYDYSSSGALSFYSVSPILGNGKTSTPSP 513

Query: 434 WGSILTG-SHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANF 493
           W ++  G   + +V QD+ SG      +D   ++ +    +   G I     SSPSLA  
Sbjct: 514 WDAMGAGVPSEFVVKQDVVSGPTRNPSQDLAPAQGENLHDNAGGGGI-----SSPSLAKL 573

Query: 494 EDLNSHASFALELLVDDCSTKSAKVFDCS--NIDGSSKGSSWSIAELEHHGSSYVVEDKF 553
           ED  S +S A ++ VDD STK+ K  + S  + D S K  +  ++ELE  G     E K 
Sbjct: 574 EDQESQSSVASDIFVDDTSTKNDKASEFSDYSTDRSYKDLNGYVSELE-LGDLCYDECKL 633

Query: 554 QRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDA 613
           Q N  + G    +  F KNS ++ P     S V+SLTSS SSLSLT KD+D ELKMEIDA
Sbjct: 634 QGNYSEDGEDILLYQFVKNSKLTFP---NLSTVLSLTSSYSSLSLTGKDVD-ELKMEIDA 693

Query: 614 IETHYRQLFDELSRMREEALEATRRRWIAKKKL 644
           IE  Y   F ELS+M+EEALEA+R+RW+AKK+L
Sbjct: 694 IEAQYEHWFQELSKMKEEALEASRKRWMAKKRL 716

BLAST of Cucsa.259440.1 vs. TAIR10
Match: AT1G49160.2 (AT1G49160.2 Protein kinase superfamily protein)

HSP 1 Score: 501.5 bits (1290), Expect = 7.7e-142
Identity = 257/406 (63.30%), Postives = 307/406 (75.62%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           +K FDEVDGIEVAWNQVRID  LQSP+ LE+LYSEV LLKSLKH+NII+FYNSW+DDK K
Sbjct: 43  FKGFDEVDGIEVAWNQVRIDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNK 102

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVN+ITELFTSGSLRQYRKKH+ V+MKA+K WARQIL GL YLHS DPPIIHRD+K DNI
Sbjct: 103 TVNIITELFTSGSLRQYRKKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNI 162

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEM
Sbjct: 163 FINGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEM 222

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPY EC+N AQI++KV+SGIKPASL+KV DP  M+FI KCL+P  ERLSA+ELL D
Sbjct: 223 VTFEYPYCECRNSAQIYKKVSSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLD 282

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPI----------------SMDIDIDQKI 313
           SFL V     +  NPL L + V     +PK G                  +M +++D+  
Sbjct: 283 SFLNVNGLVMN--NPLPLPDIV-----MPKEGSFGERCLMSEGPPNARNRTMSMNLDEDN 342

Query: 314 HSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHF 373
           +     + +NSG+     +E +   + N F L+G +ND+NSV+L LRI D NGRVRNIHF
Sbjct: 343 NLPIVISSNNSGTN---CIEVRRAKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHF 402

Query: 374 TFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWK 404
            F+ + DTA +V++EM EQLEL + +V FIAE ID L+  LIP WK
Sbjct: 403 LFFQEGDTASNVSSEMVEQLELTDKNVKFIAELIDVLLVNLIPNWK 438


HSP 2 Score: 40.0 bits (92), Expect = 6.3e-03
Identity = 19/48 (39.58%), Postives = 30/48 (62.50%), Query Frame = 1

Query: 594 SLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKK 642
           SL +++ D  LK E++ IE  +R+   E++R REEA   T+ R+  KK
Sbjct: 505 SLEEEEEDERLKEELEKIEERFREEMKEITRKREEATMETKNRFFEKK 552

BLAST of Cucsa.259440.1 vs. TAIR10
Match: AT3G18750.1 (AT3G18750.1 with no lysine (K) kinase 6)

HSP 1 Score: 500.4 bits (1287), Expect = 1.7e-141
Identity = 259/404 (64.11%), Postives = 304/404 (75.25%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRID  LQSP  LE+LYSEV LLKSLKH NII+FYNSW+DDK K
Sbjct: 43  YKAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNK 102

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVN+ITELFTSGSLR YRKKH+ V+MKA+KNWARQIL GL YLH  +PPIIHRDLK DNI
Sbjct: 103 TVNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNI 162

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEM
Sbjct: 163 FINGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEM 222

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTF+YPY ECKN AQI++KV+SGIKPASL++V DP   +FI KCL+P  ERLSAKELL D
Sbjct: 223 VTFDYPYCECKNSAQIYKKVSSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLD 282

Query: 254 SFLQVENPKESARNPLQLSNQVSKSIN------LPKSGP--------ISMDIDIDQKIHS 313
            FLQ+     +  NPL L + V           L   GP        +S+D+D D  +  
Sbjct: 283 PFLQLNG--LTMNNPLPLPDIVMPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNL-P 342

Query: 314 LSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTF 373
           + T+++ NSGS     +E +   + N F L+G +ND+ SV+L LRI D NGRVRNIHF F
Sbjct: 343 IVTFSD-NSGS---RCIEVRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLF 402

Query: 374 YLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWK 404
           Y + DTA  V++EM EQLEL + +V FIAE ID L+  +IP WK
Sbjct: 403 YQEGDTASKVSSEMVEQLELTDQNVTFIAELIDILLVNMIPTWK 439

BLAST of Cucsa.259440.1 vs. TAIR10
Match: AT5G41990.1 (AT5G41990.1 with no lysine (K) kinase 8)

HSP 1 Score: 479.2 bits (1232), Expect = 4.1e-135
Identity = 242/388 (62.37%), Postives = 294/388 (75.77%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWN V I+  +Q P  LE+LYSEVHLLK+LKHENIIK + SWVD+K K
Sbjct: 44  YKAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNK 103

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           T+NMITELFTSGSLR YRKKH+ VD KAIKNWARQIL+GL YLHS +PP+IHRDLK DNI
Sbjct: 104 TINMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNI 163

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGN GEVKIGDLGLA V+QQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEM
Sbjct: 164 FVNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEM 223

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VT EYPY+EC+N AQI++KVTS IKP SL KV DP+  +FI KCL+P   R +A EL KD
Sbjct: 224 VTCEYPYNECRNQAQIYKKVTSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKD 283

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
            FL  +  K+SA   L  S+  SK +  P+   + MD+D     H+ +    SN   P  
Sbjct: 284 PFLARDGGKDSAL--LASSSTSSKYVRPPQLEHLPMDVD-----HNENKSVSSNEDYPWS 343

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
             +E Q + +N EFRLRG ++DD + ++ LRIAD +G+ R +HF FYL+SDTA ++A EM
Sbjct: 344 QTIELQRIAENKEFRLRGERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEM 403

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPE 402
            E+L L + +V  IA+ ID  I +L+ +
Sbjct: 404 VEELHLTSQEVVVIADMIDDFIMQLLSD 424


HSP 2 Score: 59.7 bits (143), Expect = 7.7e-09
Identity = 29/84 (34.52%), Postives = 51/84 (60.71%), Query Frame = 1

Query: 565 YFAKNSVVSMPAPSEASNVMSLTSSCS------SLSLTDKDLDAELKMEIDAIETHYRQL 624
           Y A +   +M A  +A ++ S   SCS      +LS++D D   +LK E++ IE+ + Q 
Sbjct: 474 YSANDGNAAMEAGRDAESMSSYLDSCSMMSTIYNLSISDNDYPEDLKTELNLIESQFNQS 533

Query: 625 FDELSRMREEALEATRRRWIAKKK 643
           F +L +++E+A+E  +R+WI KK+
Sbjct: 534 FQDLLKLKEDAIENAKRKWITKKQ 557

BLAST of Cucsa.259440.1 vs. TAIR10
Match: AT1G64630.1 (AT1G64630.1 with no lysine (K) kinase 10)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-127
Identity = 243/444 (54.73%), Postives = 302/444 (68.02%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEV+GIEVAWN + I+  LQ P  L++LYSEVHLL SLKH+NIIK + SWVDD  K
Sbjct: 31  YKAFDEVEGIEVAWNLMSIEDVLQMPGQLDRLYSEVHLLNSLKHDNIIKLFYSWVDDHNK 90

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           ++NMITELFTSGSL  YRKKH+ VD KAI NWARQIL+GL YLHS  PP+IHRDLK DNI
Sbjct: 91  SINMITELFTSGSLTLYRKKHRKVDPKAIMNWARQILKGLHYLHSQTPPVIHRDLKCDNI 150

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           F+NGN G+VKIGDLGLA VMQQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEM
Sbjct: 151 FVNGNTGKVKIGDLGLAAVMQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEM 210

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VT EYPY EC+N AQI++KVTSGIKP SL+KV DP+  +FI KCL+P   R +A ELLKD
Sbjct: 211 VTCEYPYRECRNQAQIYKKVTSGIKPQSLSKVDDPQVKQFIEKCLLPAPSRPTALELLKD 270

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIH-SLSTYAESNSGSPR 313
             L V+  K+S       SN   K    P+     MD++  +    S+ + A+S+     
Sbjct: 271 QLLAVDGAKDSTLT--ASSNTTFKPAMPPQCEYRPMDVEYKKNTSVSICSSAKSSQECAL 330

Query: 314 FPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAE 373
              +E Q + ++ EF+L G + DD + ++ LRIA S+G+ R + F F L +DTA +V  E
Sbjct: 331 LQTMEVQRVAESTEFKLSGERRDDVAASMALRIAGSSGQARKVDFDFNLKTDTARAVTGE 390

Query: 374 MAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGS 433
           M E+L+L +H+V  IAE ID LI KL                SL +A    +S     G 
Sbjct: 391 MVEELDLSSHEVTVIAEMIDELIMKL------------KANRSLPNANSVYQSKDEEAGE 450

Query: 434 AWGSILTGSHDGLVAQDISSGLGC 457
           +  S ++  +   V+ +  S LGC
Sbjct: 451 SMKSEISADYYHRVSSNEGSRLGC 460


HSP 2 Score: 43.9 bits (102), Expect = 4.4e-04
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 1

Query: 586 LTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWI 639
           L+S   S S+       +LK E++ IE+ Y Q    L RM+EEA+E  +R+W+
Sbjct: 469 LSSFLDSCSMVSNKQSEDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of Cucsa.259440.1 vs. TAIR10
Match: AT5G28080.2 (AT5G28080.2 Protein kinase superfamily protein)

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 216/435 (49.66%), Postives = 284/435 (65.29%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           Y+ FDE  GIEVAWNQV++  FLQSP++LE+LY E+HLLK+LKH++I+KFY SWVD   +
Sbjct: 40  YRGFDEYQGIEVAWNQVKLYDFLQSPQELERLYCEIHLLKTLKHKSIMKFYASWVDTDNR 99

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
            +N +TE+FTSG+LRQYR KHK V+++A+KNW RQILRGL YLH+HDPP+IHRDLK DNI
Sbjct: 100 NINFVTEMFTSGTLRQYRLKHKRVNIRAVKNWCRQILRGLNYLHTHDPPVIHRDLKCDNI 159

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGN GEVKIGDLGLA  +Q   A   +GTPEFMAPE+Y+EEYN+LVD+YSFGMC+LEM
Sbjct: 160 FINGNQGEVKIGDLGLAACLQHSHAAHCVGTPEFMAPEVYKEEYNQLVDIYSFGMCVLEM 219

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTF+YPYSEC +PAQI+++V SG KP  L KV DP    FI KCL  V  RLSA ELL D
Sbjct: 220 VTFDYPYSECSHPAQIYKRVISGKKPDGLDKVKDPEVRGFIEKCLATVSLRLSACELLDD 279

Query: 254 SFL--------QVENPK---ESARNPLQLSNQVSKSIN----LPKSGPISMDIDIDQKIH 313
            FL        +VE+ K   + A  PL+ S  +    N    L        + D   + H
Sbjct: 280 HFLCIDESDMRRVESEKGLIDEAGTPLRHSYHIPHYSNGYYSLYNQNQWDYNGDETVESH 339

Query: 314 SLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDD-NSVALTLRIADSNGRVRNIHF 373
            +      N          F ++    +  ++G + D+ + + L L+  +  G VRNI+F
Sbjct: 340 EIDLLEFQNDDDEEEEDKRFGSV----DISIKGKRRDNGDGLFLRLKTVNKEGCVRNIYF 399

Query: 374 TFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLF 433
            F +++DTA+SVA EM E+LE+ + DV  IA  ID  I  L+P W   S++ S+      
Sbjct: 400 PFDIETDTAISVAREMVEELEMDDRDVTKIANMIDGEIASLVPNW---SIFCSS------ 455

BLAST of Cucsa.259440.1 vs. NCBI nr
Match: gi|778676816|ref|XP_011650666.1| (PREDICTED: probable serine/threonine-protein kinase WNK7 isoform X2 [Cucumis sativus])

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 645/645 (100.00%), Postives = 645/645 (100.00%), Query Frame = 1

Query: 1   MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENI 60
           MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENI
Sbjct: 1   MLLKKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENI 60

Query: 61  IKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHD 120
           IKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHD
Sbjct: 61  IKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHD 120

Query: 121 PPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNEL 180
           PPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNEL
Sbjct: 121 PPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNEL 180

Query: 181 VDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVP 240
           VDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVP
Sbjct: 181 VDVYSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVP 240

Query: 241 VHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSL 300
           VHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSL
Sbjct: 241 VHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSL 300

Query: 301 STYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFY 360
           STYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFY
Sbjct: 301 STYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFY 360

Query: 361 LDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAP 420
           LDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAP
Sbjct: 361 LDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAP 420

Query: 421 PFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIF 480
           PFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIF
Sbjct: 421 PFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIF 480

Query: 481 DTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHH 540
           DTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHH
Sbjct: 481 DTCPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHH 540

Query: 541 GSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDL 600
           GSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDL
Sbjct: 541 GSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDL 600

Query: 601 DAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLIH 646
           DAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLIH
Sbjct: 601 DAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLIH 645

BLAST of Cucsa.259440.1 vs. NCBI nr
Match: gi|449433018|ref|XP_004134295.1| (PREDICTED: probable serine/threonine-protein kinase WNK7 isoform X1 [Cucumis sativus])

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 632/632 (100.00%), Postives = 632/632 (100.00%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK
Sbjct: 32  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 91

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI
Sbjct: 92  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 151

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM
Sbjct: 152 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 211

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD
Sbjct: 212 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 271

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
           SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF
Sbjct: 272 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 331

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
           PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM
Sbjct: 332 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 391

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 433
           AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA
Sbjct: 392 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 451

Query: 434 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 493
           WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE
Sbjct: 452 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 511

Query: 494 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 553
           DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN
Sbjct: 512 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 571

Query: 554 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 613
           VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET
Sbjct: 572 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 631

Query: 614 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 646
           HYRQLFDELSRMREEALEATRRRWIAKKKLIH
Sbjct: 632 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 663

BLAST of Cucsa.259440.1 vs. NCBI nr
Match: gi|700201270|gb|KGN56403.1| (hypothetical protein Csa_3G119370 [Cucumis sativus])

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 632/632 (100.00%), Postives = 632/632 (100.00%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK
Sbjct: 102 YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 161

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
           TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI
Sbjct: 162 TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 221

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM
Sbjct: 222 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 281

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD
Sbjct: 282 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 341

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
           SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF
Sbjct: 342 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 401

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
           PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM
Sbjct: 402 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 461

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 433
           AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA
Sbjct: 462 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 521

Query: 434 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 493
           WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE
Sbjct: 522 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 581

Query: 494 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 553
           DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN
Sbjct: 582 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 641

Query: 554 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 613
           VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET
Sbjct: 642 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 701

Query: 614 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 646
           HYRQLFDELSRMREEALEATRRRWIAKKKLIH
Sbjct: 702 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 733

BLAST of Cucsa.259440.1 vs. NCBI nr
Match: gi|659074907|ref|XP_008437860.1| (PREDICTED: probable serine/threonine-protein kinase WNK7 [Cucumis melo])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 599/632 (94.78%), Postives = 607/632 (96.04%), Query Frame = 1

Query: 14  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 73
           YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK
Sbjct: 32  YKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 91

Query: 74  TVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 133
            VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI
Sbjct: 92  NVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNI 151

Query: 134 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 193
           FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM
Sbjct: 152 FINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEM 211

Query: 194 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLKD 253
           VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELL+D
Sbjct: 212 VTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHERLSAKELLQD 271

Query: 254 SFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTYAESNSGSPRF 313
           SFLQVENPKESARNPLQLSNQVS+SINLPKSGPISMDIDID K+HSLS YAESNSGSPRF
Sbjct: 272 SFLQVENPKESARNPLQLSNQVSRSINLPKSGPISMDIDIDHKLHSLSNYAESNSGSPRF 331

Query: 314 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDSDTALSVAAEM 373
           PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIAD NGRVRNIHFTFYLDSDTALSVAAEM
Sbjct: 332 PVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADPNGRVRNIHFTFYLDSDTALSVAAEM 391

Query: 374 AEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFLKSAKSSIGSA 433
           AEQLELINHDVDFIAEFIDFLITKLIP WKPLSVYSSNGE SL SAPP LKSAKSS+ S 
Sbjct: 392 AEQLELINHDVDFIAEFIDFLITKLIPGWKPLSVYSSNGESSLCSAPPVLKSAKSSVRSP 451

Query: 434 WGSILTGSHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDTCPSSPSLANFE 493
           WGS+LTGSHDGLVAQD SSG+ CGTQ D LQSE +GWT DIS GHIFDTCPSSPSLANFE
Sbjct: 452 WGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQSERNGWTGDISGGHIFDTCPSSPSLANFE 511

Query: 494 DLNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHHGSSYVVEDKFQRN 553
           D NSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEH GSSY VEDK QRN
Sbjct: 512 DSNSHASFALELLVDDCSTKSAKVFDCSNIDGSSKGSSWSIAELEHQGSSY-VEDKLQRN 571

Query: 554 VGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDLDAELKMEIDAIET 613
           V  VGIFTP+DYF KNSVVSMPAPSEASNVMSLTSSCSSLSL DKDLDAELKMEIDAIET
Sbjct: 572 VSGVGIFTPLDYFVKNSVVSMPAPSEASNVMSLTSSCSSLSLADKDLDAELKMEIDAIET 631

Query: 614 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 646
           HYRQLFDELSRMREEALEATRRRWIAKKKLIH
Sbjct: 632 HYRQLFDELSRMREEALEATRRRWIAKKKLIH 662

BLAST of Cucsa.259440.1 vs. NCBI nr
Match: gi|641867852|gb|KDO86536.1| (hypothetical protein CISIN_1g005017mg [Citrus sinensis])

HSP 1 Score: 773.9 bits (1997), Expect = 2.3e-220
Identity = 419/643 (65.16%), Postives = 486/643 (75.58%), Query Frame = 1

Query: 4   KKILPVDTFGYKAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKF 63
           ++I  VDT  YKAFDE  GIEVAW++V+ID  LQ P DL+KLYSEVHLLKSLK +N++KF
Sbjct: 8   RRIPAVDT--YKAFDEFAGIEVAWSRVKIDDVLQLPGDLQKLYSEVHLLKSLKDDNVVKF 67

Query: 64  YNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPI 123
           Y SWVDDKKKTVNMITELFTSG+LRQYRKKHK+VD+K IKNWARQIL GLVYLHSH+PPI
Sbjct: 68  YKSWVDDKKKTVNMITELFTSGNLRQYRKKHKNVDIKVIKNWARQILHGLVYLHSHNPPI 127

Query: 124 IHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDV 183
           IHRDLK DNIF+NGNHGEVKIGDLGLAI MQQPTARSVIGTPEFMAPELYEEEYNELVD+
Sbjct: 128 IHRDLKCDNIFVNGNHGEVKIGDLGLAIAMQQPTARSVIGTPEFMAPELYEEEYNELVDI 187

Query: 184 YSFGMCMLEMVTFEYPYSECKNPAQIFRKVTSGIKPASLAKVSDPRTMEFINKCLVPVHE 243
           YSFGMC+LEMVTFEYPYSECKNPAQIF+KVTSGIKPASLAKV+DP+   FI KCLVP  E
Sbjct: 188 YSFGMCILEMVTFEYPYSECKNPAQIFKKVTSGIKPASLAKVNDPQIKGFIEKCLVPASE 247

Query: 244 RLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKSGPISMDIDIDQKIHSLSTY 303
           RLSAK+LLKD FLQVEN KE   +PL+L  Q  K + LP SGP SMDID D K  SLST 
Sbjct: 248 RLSAKDLLKDPFLQVENQKEPICDPLKLPIQSLKMLRLPMSGPSSMDIDSDYKQLSLSTC 307

Query: 304 AESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSNGRVRNIHFTFYLDS 363
            ESN+GSP  PV+EFQ M+KNNEFRLRG KNDD SV+LTLRIADS+GRVRNIHF FYLDS
Sbjct: 308 TESNNGSPHCPVLEFQRMHKNNEFRLRGTKNDDTSVSLTLRIADSSGRVRNIHFLFYLDS 367

Query: 364 DTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKPLSVYSSNGELSLFSAPPFL 423
           DTALSVA EM EQL+L +HDV FIAEFID+LI KL+P WKP   YSS+G LS +S  P L
Sbjct: 368 DTALSVAGEMVEQLDLADHDVAFIAEFIDYLIMKLLPGWKPSYDYSSSGALSFYSVSPIL 427

Query: 424 KSAKSSIGSAWGSILTG-SHDGLVAQDISSGLGCGTQKDCLQSEEDGWTTDISAGHIFDT 483
            + K+S  S W ++  G   + +V QD+ SG      +D   ++ +    +   G I   
Sbjct: 428 GNGKTSTPSPWDAMGAGVPSEFVVKQDVVSGPTRNPSQDLAPAQGENLHDNAGGGGI--- 487

Query: 484 CPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCS--NIDGSSKGSSWSIAELEHH 543
             SSPSLA  ED  S +S A ++ VDD STK+ K  + S  + D S K  +  ++ELE  
Sbjct: 488 --SSPSLAKLEDQESQSSVASDIFVDDTSTKNDKASEFSDYSTDRSYKDLNGYVSELE-L 547

Query: 544 GSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNVMSLTSSCSSLSLTDKDL 603
           G     E K Q N  + G    +  F KNS ++ P     S V+SLTSS SSLSLT KD+
Sbjct: 548 GDLCYDECKLQGNYSEDGEDILLYQFVKNSKLTFP---NLSTVLSLTSSYSSLSLTGKDV 607

Query: 604 DAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 644
           D ELKMEIDAIE  Y   F ELS+M+EEALEA+R+RW+AKK+L
Sbjct: 608 D-ELKMEIDAIEAQYEHWFQELSKMKEEALEASRKRWMAKKRL 638

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WNK4_ORYSJ5.9e-14466.15Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica GN... [more]
WNK7_ARATH1.4e-14063.30Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana GN=WNK7 PE... [more]
WNK6_ARATH3.0e-14064.11Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana GN=WNK6 PE... [more]
WNK8_ARATH7.2e-13462.37Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana GN=WNK8 PE=1 SV=1[more]
WNK10_ARATH1.9e-12654.73Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana GN=WNK10 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L3S7_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G119370 PE=4 SV=1[more]
A0A067HF81_CITSI1.6e-22065.16Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1[more]
V4TWU9_9ROSI6.0e-22064.55Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019052mg PE=4 SV=1[more]
A0A067HEX2_CITSI6.0e-22064.55Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1[more]
A0A067H6I0_CITSI7.8e-22065.56Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005017mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G49160.27.7e-14263.30 Protein kinase superfamily protein[more]
AT3G18750.11.7e-14164.11 with no lysine (K) kinase 6[more]
AT5G41990.14.1e-13562.37 with no lysine (K) kinase 8[more]
AT1G64630.11.1e-12754.73 with no lysine (K) kinase 10[more]
AT5G28080.21.1e-11149.66 Protein kinase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778676816|ref|XP_011650666.1|0.0e+00100.00PREDICTED: probable serine/threonine-protein kinase WNK7 isoform X2 [Cucumis sat... [more]
gi|449433018|ref|XP_004134295.1|0.0e+00100.00PREDICTED: probable serine/threonine-protein kinase WNK7 isoform X1 [Cucumis sat... [more]
gi|700201270|gb|KGN56403.1|0.0e+00100.00hypothetical protein Csa_3G119370 [Cucumis sativus][more]
gi|659074907|ref|XP_008437860.1|0.0e+0094.78PREDICTED: probable serine/threonine-protein kinase WNK7 [Cucumis melo][more]
gi|641867852|gb|KDO86536.1|2.3e-22065.16hypothetical protein CISIN_1g005017mg [Citrus sinensis][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.259440Cucsa.259440gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.259440.1Cucsa.259440.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.259440.1.five_prime_UTR.1Cucsa.259440.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.259440.1.CDS.1Cucsa.259440.1.CDS.1CDS
Cucsa.259440.1.CDS.2Cucsa.259440.1.CDS.2CDS
Cucsa.259440.1.CDS.3Cucsa.259440.1.CDS.3CDS
Cucsa.259440.1.CDS.4Cucsa.259440.1.CDS.4CDS
Cucsa.259440.1.CDS.5Cucsa.259440.1.CDS.5CDS
Cucsa.259440.1.CDS.6Cucsa.259440.1.CDS.6CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 14..256
score: 3.1
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 6..256
score: 1.6
IPR000719Protein kinase domainPROFILEPS50011PROTEIN_KINASE_DOMcoord: 1..256
score: 37
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 123..135
scor
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 14..257
score: 2.02
NoneNo IPR availableunknownCoilCoilcoord: 608..635
scor
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 100..274
score: 3.7
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 339..397
score: 6.5
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 14..99
score: 1.1
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 14..261
score: 3.4E-269coord: 311..415
score: 3.4E
NoneNo IPR availablePANTHERPTHR13902:SF58SUBFAMILY NOT NAMEDcoord: 14..261
score: 3.4E-269coord: 311..415
score: 3.4E