Cucsa.242070.2 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.242070.2
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Co-chaperone protein HscB, mitochondrial, putative
Location	scaffold02047 : 1900171 .. 1903682 (+)
Sequence length	1236

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAGTTACGAACAATTCGGACCGATTCTTCTTCCAATGTTGAAGATGAAGCTTCGATCTTCACTTTTCTACGTTCTACGCACCAACACACCTCTCTCTTCCGCCATTTATTTCCCAAATGTTTCCAATTCGGCTCTATCATCCCAAATTTCTTCAATTTCACTACTTCCTTCCATCGCTGAATGTTCTCGGCCTTCGCCACCCACTGCATGTGAATTTCTCGACAATCTGAGCTTTTCCTCCAGGGTTTTCTGCTCCCGGTCGGCAGAGGCGTCCGTCCTTAAACCTGGGTGCTGGAATTGCGGTGCGGTGGCTCCATCGTCGGCGGTGTTTCTGGTGTGCGATTCTTGTCGGAGCATTCAGCCTGTGGACCAGTTGGTGGATTATTTTCAGATATTTGGATTGTAAGTGTATAGAATAATGAGGGTTGCTTTACGCTTTCTTGATTTATGTTAACTTTGTATTGGTTGCTGAGGAAAGTGAGTAGAAGTTGGAATTTGATTGTCTGCTGATTATCGGAAGTGATGAAAGATTTGAACTTAGGGCTTGATGGGTCGGTCTGTTGTAATCCATTTTCTTTGAAACTTGTTGGGCTCAACTCGAATGAAAGTTTTCTGATGTATTGATTCGTTACAAAGCTTCTCTCTCTGTTCTATCCTGTGTGAATGTACCTCTTACCTCTAATTACTCAAGGTCCATGGAAAATTGGGATAATGGTTTTtCTTTTTAATTTGGAGAATTGATGCAATCAACTGGTCATGTTTCTTATCTTAAACCTTCAGGCTCACTCGCTGGAGGACATATGGAATGTCTTCTCTGACTCATTGCTAGTTGGTGGGTTATAGAATTCCCGAACGAAGTGATGTATAATTAGAATTCTTAAATCTTTTTCCAGGGAAAAGGAGTATGAAATTGGAGATGTCAATCTGGAGAGCAAGTATAAAGATTGGCAGAAGAAATTACATCCCGACTTAGTTCATTCTAAATCAGACGTCTGTCTCATTTTACTTTTGTGAAGTTTGGTAGTCTTTGAGGGGCTCTAATTTTTATCTTCCCGTGTTTTATAGAGAGAAAGAGAATATGCTGCTGAACAATCTGCTCGGGTGATCGATGCTTACCGTACTCTTAGTAAGCCATTGTCGAGAGCAATATATATTGTAAGTGTGCATGTCTACCGTAGGATTTTTTTAAGCTTATTTGTTGGATTTCGATTTAAAGTTTTTGTGACAGTGGGATATATTTGTCGTTTTTCGGTTTGTTAGAATTATAAGAGTTTGCTTACTGGAAATTTGTCATTGTATTTCAGTATTATTGTCTACCTTTGGTTTATTTTGTTTCTCTCTTCTCCAGTTATTACTTTTTTCATTTGAGTATTAGTCTCTTTTCATTTTTTAAAAGAAAAGTTAACGTTTCCTTTTAAAATAGATTCCATGTTATCAGCCATTTCCACCTCCAAATACTTCAAGTTTTATGATTCAAGCTGGGTAAGTGTAATAAAACATTCTCAGGAAATTTCTGTATCAACTTTTCTTTTtAGTTCCTTTGCAACCCCATCCCACTTAGCACTTACATTATAAATGGAAGTGATGGACCAACATGTGATGGTAATTATAAATTTATAATTGATTGTGCTTCTTCCTGACAATTATAAACTATGCTGCGGAAGAATTGATTACAGCTATTTTATATGATTTGGTATTTTGGAGTTAAATGTCTTTCGATGCACTGGGTGGAGGTTGCTTTTTCATTTTTTCCTCAAGAGACAGAGCACATCGCTCAAATATAGAGCAAGTCTTCTGAGTCCTTGAACGGGAGGGACTTGTTTATAAGGTGTAATTGGAAAGATGTACAGACTTGCACTTTAAGCATTAATCCCGAGTGATGTTTAGTGTTGGGATCAGATAACAGACAAAGCCTTTAGAAGGGTTTACTTTTTTtGCCCTACCTACACCAATTTTATTTCCTACTTGGACTCTTATATTAGTCCATTACTCCGTATAACATTTGCCCTCCTGAAGAGCAACGTTGCTGGTTTGTGGTTAGAACTTGAAATATTCCTCATAGGGCCTTTTTtTTtACAATTACAATTATAATTATGTAAAGCTCAACCAATCATTTATATTCTGGAATTAATTTATTTTTTCTTATATTTATGAGACATTTGCATCTCTGTAGTTTGATGTTTGTGATCTTGGTTTTTATGCATTATGTTTATGCCATCTGCAATCTAGAATTTCAGTTTGAACATTTTGTATTGGTCTTGGTGATAGTTATAGGCTTCCTCTCCAACTTGTAAATTATTGGTCTTCCCCTTCAATGAGATTGTTGTATTTTCTTGCCATTCTTTTCAGCTGAAACTCGAGGGTGTAGATGTTGATGAAGAGGATACAATTTCTGAACCAGAGTTGCTAAATGAGGTAATTAGTAAGACCTTAAATTCGTCCCTGTCTGAGTTATTCAGTTCTGTGAGAGCTTTTAGCTTTTTTtTTTTtGTGAGCATTTTATGTGATGAGTTATGGATTATGAGAAGAAAGTCAAACACATTCGACTGATTTTCAATGTCTGACTGCAAGCTTGTGTTCTGTTTAAAGTTTGATTTATATGAAGTTACCAAACCCTTTTCGCAGATTATGGAAATCAGGGAAGCTGTAGAAGACGCATCTGGATCTCAGGAATTAAACCAGATTCAGTCACAGGTGCTACTCAATGCTCATTTTGTTCATCATCTTTATCTTTCTTTGGTTAAATTACAATTTTGGTCCCTGAAGTTCGGAGAAAGTTAGAATTTAGTATCTATGATTTTTAAAGTTAGAATTTAGTTCTTATGATTCGATAAAACCTCATAAATAAAATTCTCCTCTATAGTCTTAGTGGATTTTATCAAACCTTGGGTCTTAATTTTAATTGGAACCGAGACTAAATTTGTAATCTAACCTCCTTTCTCTTGAATCTTCGTTCATAAATTCACGTGGAAGATTGAAAGTGGAAGTTGCATTTAAAGAATTAAAATAGCATGTAATGATGCCTAAACCTGTTTCATTTTtCTTTTTtGTTCATCTAGATGCAAGAGAAGTTAAATCATTGGTCAAACACATTTGCAAAAGCTTTGCGAGACAGGAACTTCAACGATGCTGTCTCGTCCATACAGCGAATGACATACTATGAGCGGGTGAAAGAAGAAATTACAAAGAAGCTTTAGTGCAGGAGGTGTAAAATCGATGGAAGCACTGTGTAGCTTATCCACAAAATTCCCACAAAAGAATTCTTCGATCTTTTATCATCTAACCTCTTCATCATATGCTTGCTTATACTTCAGCTTTTAAGTATTAAAAAAGTTCATAGATTGGTGGTAAAATATTTCATACCAATTTTGTAATTCTGATGTTGCTGAATGCTTACTGGTAGTCTCAGCTGCACCAATAAAGAGAGACAGATTAACTATTAGAAATGTTGAGAAACAGTTTACATATAATAATATTTAATTAATAAATATTCATTAATTTGTATTGTTT

mRNA sequence

TTCAGTTACGAACAATTCGGACCGATTCTTCTTCCAATGTTGAAGATGAAGCTTCGATCTTCACTTTTCTACGTTCTACGCACCAACACACCTCTCTCTTCCGCCATTTATTTCCCAAATGTTTCCAATTCGGCTCTATCATCCCAAATTTCTTCAATTTCACTACTTCCTTCCATCGCTGAATGTTCTCGGCCTTCGCCACCCACTGCATGTGAATTTCTCGACAATCTGAGCTTTTCCTCCAGGGTTTTCTGCTCCCGGTCGGCAGAGGCGTCCGTCCTTAAACCTGGGTGCTGGAATTGCGGTGCGGTGGCTCCATCGTCGGCGGTGTTTCTGGTGTGCGATTCTTGTCGGAGCATTCAGCCTGTGGACCAGTTGGTGGATTATTTTCAGATATTTGGATTGGAAAAGGAGTATGAAATTGGAGATGTCAATCTGGAGAGCAAGTATAAAGATTGGCAGAAGAAATTACATCCCGACTTAGTTCATTCTAAATCAGACAGAGAAAGAGAATATGCTGCTGAACAATCTGCTCGGGTGATCGATGCTTACCGTACTCTTAGTAAGCCATTGTCGAGAGCAATATATATTATTCCATGTTATCAGCCATTTCCACCTCCAAATACTTCAAGTTTTATGATTCAAGCTGGCTGAAACTCGAGGGTGTAGATGTTGATGAAGAGGATACAATTTCTGAACCAGAGTTGCTAAATGAGATTATGGAAATCAGGGAAGCTGTAGAAGACGCATCTGGATCTCAGGAATTAAACCAGATTCAGTCACAGATGCAAGAGAAGTTAAATCATTGGTCAAACACATTTGCAAAAGCTTTGCGAGACAGGAACTTCAACGATGCTGTCTCGTCCATACAGCGAATGACATACTATGAGCGGGTGAAAGAAGAAATTACAAAGAAGCTTTAGTGCAGGAGGTGTAAAATCGATGGAAGCACTGTGTAGCTTATCCACAAAATTCCCACAAAAGAATTCTTCGATCTTTTATCATCTAACCTCTTCATCATATGCTTGCTTATACTTCAGCTTTTAAGTATTAAAAAAGTTCATAGATTGGTGGTAAAATATTTCATACCAATTTTGTAATTCTGATGTTGCTGAATGCTTACTGGTAGTCTCAGCTGCACCAATAAAGAGAGACAGATTAACTATTAGAAATGTTGAGAAACAGTTTACatataataatatttaattaataaatattcattaatttGTATTGTTT

Coding sequence (CDS)

ATGTTGAAGATGAAGCTTCGATCTTCACTTTTCTACGTTCTACGCACCAACACACCTCTCTCTTCCGCCATTTATTTCCCAAATGTTTCCAATTCGGCTCTATCATCCCAAATTTCTTCAATTTCACTACTTCCTTCCATCGCTGAATGTTCTCGGCCTTCGCCACCCACTGCATGTGAATTTCTCGACAATCTGAGCTTTTCCTCCAGGGTTTTCTGCTCCCGGTCGGCAGAGGCGTCCGTCCTTAAACCTGGGTGCTGGAATTGCGGTGCGGTGGCTCCATCGTCGGCGGTGTTTCTGGTGTGCGATTCTTGTCGGAGCATTCAGCCTGTGGACCAGTTGGTGGATTATTTTCAGATATTTGGATTGGAAAAGGAGTATGAAATTGGAGATGTCAATCTGGAGAGCAAGTATAAAGATTGGCAGAAGAAATTACATCCCGACTTAGTTCATTCTAAATCAGACAGAGAAAGAGAATATGCTGCTGAACAATCTGCTCGGGTGATCGATGCTTACCGTACTCTTAGTAAGCCATTGTCGAGAGCAATATATATTATTCCATGTTATCAGCCATTTCCACCTCCAAATACTTCAAGTTTTATGATTCAAGCTGGCTGA

Protein sequence

MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACEFLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSRAIYIIPCYQPFPPPNTSSFMIQAG*

Homology

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BLAST of Cucsa.242070.2 vs. Swiss-Prot
Match: HSC20_MOUSE (Iron-sulfur cluster co-chaperone protein HscB, mitochondrial OS=Mus musculus GN=Hscb PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 5.7e-16
Identity = 39/118 (33.05%), Postives = 62/118 (52.54%), Query Frame = 1


Query: 70  RVFCSRSAEASVLKPGCWNCG-AVAPSSAVFLVCDSCRSIQPVDQLVDYFQIFGLEKEYE 129
           R+  S +A    + P CWNCG A          C  CR++QP D   DYF +    + + 
Sbjct: 24  RLLGSNAAAGKSIAPQCWNCGHAREAGCGDEFFCSHCRALQPPDPTRDYFSLMNCNRSFR 83

Query: 130 IGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSRAIYII 187
           +    L+ +Y+  Q+ +HPD    KS  E+ ++ + S  V DAY+TL  PL+R +Y++
Sbjct: 84  VDVTKLQHRYQQLQRLVHPDFFSQKSQTEKHFSDKHSTLVNDAYKTLQAPLTRGLYLL 141

[Close]

BLAST of Cucsa.242070.2 vs. Swiss-Prot
Match: HSCB_SERP5 (Co-chaperone protein HscB OS=Serratia proteamaculans (strain 568) GN=hscB PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.6e-10
Identity = 31/72 (43.06%), Postives = 47/72 (65.28%), Query Frame = 1


Query: 115 VDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRT 174
           +DYF +FGL   Y +    L S+Y+D Q++ HPD   S+ +RER  A +Q+A + +AY+T
Sbjct: 1   MDYFTLFGLPVRYPVDGSLLASRYQDLQRQFHPDRFASQPERERLMALQQAATINEAYQT 60

Query: 175 LSKPLSRAIYII 187
           L  PL RA Y++
Sbjct: 61  LKHPLKRAEYML 72

[Close]

BLAST of Cucsa.242070.2 vs. Swiss-Prot
Match: HSCB_VIBCH (Co-chaperone protein HscB homolog OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN=hscB PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.0e-09
Identity = 29/72 (40.28%), Postives = 50/72 (69.44%), Query Frame = 1


Query: 115 VDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRT 174
           ++YF++FGL  ++E+    L S+++  QK+ HPD   + S+R+R  A +Q+A++ DAY+T
Sbjct: 1   MNYFELFGLPIQFELDGSLLSSQFRALQKRFHPDNFATASERDRLMAVQQAAQINDAYQT 60

Query: 175 LSKPLSRAIYII 187
           L  PL RA Y++
Sbjct: 61  LKDPLRRAEYLL 72

[Close]

BLAST of Cucsa.242070.2 vs. Swiss-Prot
Match: HSCB_VIBC3 (Co-chaperone protein HscB homolog OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) GN=hscB PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.0e-09
Identity = 29/72 (40.28%), Postives = 50/72 (69.44%), Query Frame = 1


Query: 115 VDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRT 174
           ++YF++FGL  ++E+    L S+++  QK+ HPD   + S+R+R  A +Q+A++ DAY+T
Sbjct: 1   MNYFELFGLPIQFELDGSLLSSQFRALQKRFHPDNFATASERDRLMAVQQAAQINDAYQT 60

Query: 175 LSKPLSRAIYII 187
           L  PL RA Y++
Sbjct: 61  LKDPLRRAEYLL 72

[Close]

BLAST of Cucsa.242070.2 vs. Swiss-Prot
Match: HSCB_COLP3 (Co-chaperone protein HscB homolog OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=hscB PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 29/72 (40.28%), Postives = 47/72 (65.28%), Query Frame = 1


Query: 115 VDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRT 174
           ++YFQ+F +E  +++    L S Y+  QK +HPD     S++E+  A ++SA++ DAY+T
Sbjct: 1   MNYFQLFNIEVSFDVDLQQLSSSYQTLQKTVHPDKFAHASEQEQRIAVQKSAQINDAYQT 60

Query: 175 LSKPLSRAIYII 187
           L  PL RA YI+
Sbjct: 61  LKNPLQRAEYIL 72

[Close]

BLAST of Cucsa.242070.2 vs. TrEMBL
Match: A0A0A0LBM6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G782800 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 5.8e-100
Identity = 185/186 (99.46%), Postives = 186/186 (100.00%), Query Frame = 1


Query: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60
           MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE
Sbjct: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60

Query: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120
           FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI
Sbjct: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120

Query: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180
           FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS
Sbjct: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180

Query: 181 RAIYII 187
           RAIYI+
Sbjct: 181 RAIYIL 186

[Close]

BLAST of Cucsa.242070.2 vs. TrEMBL
Match: A0A061EVT7_THECC (DNAJ heat shock N-terminal domain-containing protein, putative isoform 1 OS=Theobroma cacao GN=TCM_023091 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-45
Identity = 106/185 (57.30%), Postives = 125/185 (67.57%), Query Frame = 1


Query: 5   KLRSSLFYVLRTNTPLSSAI--YFPNVSNSALSSQISSISLLPSIAECSRPSPPTACEFL 64
           KL + L  V+R   P +  +  Y PN    + SS++ + S +               +FL
Sbjct: 6   KLWTPLSTVVRRALPTTCHLTPYSPNSQIRSSSSRLETSSRVSLSLHYGH-------DFL 65

Query: 65  DNLSFSSRVFCSRSA-EASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQIF 124
            NL FS++ FCS+SA +       CWNC AVA   A FL CDSCRSIQPVD  VDYFQIF
Sbjct: 66  GNLKFSAKSFCSQSAAKVDATTKRCWNCDAVAAGMAPFLYCDSCRSIQPVDFSVDYFQIF 125

Query: 125 GLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSR 184
           GLEK+YEI    LE KYKDWQKKLHPDLVHSKS++EREYAAEQSARVIDAYRTLSKPL+R
Sbjct: 126 GLEKKYEIEVDGLEGKYKDWQKKLHPDLVHSKSEKEREYAAEQSARVIDAYRTLSKPLAR 183

Query: 185 AIYII 187
           AIYI+
Sbjct: 186 AIYIL 183

[Close]

BLAST of Cucsa.242070.2 vs. TrEMBL
Match: A0A061EV47_THECC (DNAJ heat shock N-terminal domain-containing protein, putative isoform 2 (Fragment) OS=Theobroma cacao GN=TCM_023091 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 5.3e-45
Identity = 94/128 (73.44%), Postives = 104/128 (81.25%), Query Frame = 1


Query: 60  EFLDNLSFSSRVFCSRSA-EASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYF 119
           +FL NL FS++ FCS+SA +       CWNC AVA   A FL CDSCRSIQPVD  VDYF
Sbjct: 20  DFLGNLKFSAKSFCSQSAAKVDATTKRCWNCDAVAAGMAPFLYCDSCRSIQPVDFSVDYF 79

Query: 120 QIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKP 179
           QIFGLEK+YEI    LE KYKDWQKKLHPDLVHSKS++EREYAAEQSARVIDAYRTLSKP
Sbjct: 80  QIFGLEKKYEIEVDGLEGKYKDWQKKLHPDLVHSKSEKEREYAAEQSARVIDAYRTLSKP 139

Query: 180 LSRAIYII 187
           L+RAIYI+
Sbjct: 140 LARAIYIL 147

[Close]

BLAST of Cucsa.242070.2 vs. TrEMBL
Match: D7U8Q6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0032g00960 PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.4e-44
Identity = 104/174 (59.77%), Postives = 129/174 (74.14%), Query Frame = 1


Query: 18  TPLSSAIY--FPNVS-NSALSSQISSISLLP--SIAECSRPSPPTACEFLDNLSFSSRVF 77
           TPLS  +   FP+ S NS L+   S+I   P  S A+ SR    ++ +FL +L FS ++ 
Sbjct: 8   TPLSPILRRRFPSFSLNSTLNIPSSTIFSSPCSSTAQYSRVFWSSSYDFLGSLKFSRKLC 67

Query: 78  CSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQIFGLEKEYEIGDV 137
            ++SAE   ++  CWNC A A ++  FL CDSCRS+QPVD  VDYFQIFGLEK+YE+ D 
Sbjct: 68  SAQSAEQVHVR--CWNCKAAAQTTP-FLACDSCRSVQPVDPSVDYFQIFGLEKKYELEDN 127

Query: 138 NLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSRAIYII 187
           NLE KYKDWQKKLHPDLVHSKS+ E+EYAAEQSARVIDAY+TLSKPL+RAIYI+
Sbjct: 128 NLEGKYKDWQKKLHPDLVHSKSEEEKEYAAEQSARVIDAYQTLSKPLARAIYIL 178

[Close]

BLAST of Cucsa.242070.2 vs. TrEMBL
Match: I1L9M4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_10G083800 PE=4 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 7.7e-44
Identity = 101/189 (53.44%), Postives = 122/189 (64.55%), Query Frame = 1


Query: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60
           M K KL + L  +LR         +  N S   LS   SS+   P       PS    C+
Sbjct: 1   MSKNKLCTPLSTLLRRTLTSFPQHFTSNFSQIKLSLHDSSLPFAP-------PSHNPTCD 60

Query: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120
           +  +L F  +  CS+SAE   ++ GCWNC  V P S  FLVC+SCR IQPVD  VDYF I
Sbjct: 61  YYYSLGFFRKSLCSKSAEE--IQRGCWNCHTV-PHSEPFLVCESCRCIQPVDSSVDYFDI 120

Query: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180
           FG+E++Y+I   NLE KYKDWQKKLHPDLVHSKS +ER++AAEQSARVIDAYRTLSKPLS
Sbjct: 121 FGVERKYDIEGENLEGKYKDWQKKLHPDLVHSKSQKERDFAAEQSARVIDAYRTLSKPLS 179

Query: 181 RAIYIIPCY 190
           R IY++  Y
Sbjct: 181 RGIYLLKLY 179

[Close]

BLAST of Cucsa.242070.2 vs. TAIR10
Match: AT5G06410.1 (AT5G06410.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-41
Identity = 87/152 (57.24%), Postives = 105/152 (69.08%), Query Frame = 1


Query: 35  SSQISSISLLPSIAECSRPSPPTACEFLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAP 94
           ++Q +S++ + S  +  R S          L FS RVFCS S        GCWNCG    
Sbjct: 22  TNQCNSLATIQSQTQLPRESLQHHSSAEGRLRFSGRVFCSESGA------GCWNCG---- 81

Query: 95  SSAVFLVCDSCRSIQPVDQLVDYFQIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKS 154
             A FL C+SCRSIQPVD  VDYFQIFGLEK+YEI   +LE KYKDWQKKLHPDLVH+KS
Sbjct: 82  EKAAFLFCNSCRSIQPVDDSVDYFQIFGLEKKYEIDPGSLEGKYKDWQKKLHPDLVHNKS 141

Query: 155 DREREYAAEQSARVIDAYRTLSKPLSRAIYII 187
            +ER+YAAEQSA+V +A RTL+K LSRA+YI+
Sbjct: 142 KKERDYAAEQSAKVTEACRTLTKRLSRAMYIM 163

[Close]

BLAST of Cucsa.242070.2 vs. NCBI nr
Match: gi|449437496|ref|XP_004136528.1| (PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 8.3e-100
Identity = 185/186 (99.46%), Postives = 186/186 (100.00%), Query Frame = 1


Query: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60
           MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE
Sbjct: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60

Query: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120
           FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI
Sbjct: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120

Query: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180
           FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS
Sbjct: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180

Query: 181 RAIYII 187
           RAIYI+
Sbjct: 181 RAIYIL 186

[Close]

BLAST of Cucsa.242070.2 vs. NCBI nr
Match: gi|659084555|ref|XP_008442950.1| (PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Cucumis melo])

HSP 1 Score: 348.2 bits (892), Expect = 9.8e-93
Identity = 174/186 (93.55%), Postives = 177/186 (95.16%), Query Frame = 1


Query: 1   MLKMKLRSSLFYVLRTNTPLSSAIYFPNVSNSALSSQISSISLLPSIAECSRPSPPTACE 60
           MLK KLRSSLFYVLRTNTPLSSAI FPNVSNSALS QISS S LP+I+ECSRPSP TACE
Sbjct: 1   MLKTKLRSSLFYVLRTNTPLSSAINFPNVSNSALSPQISSFSQLPTISECSRPSPSTACE 60

Query: 61  FLDNLSFSSRVFCSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQI 120
           FLDNLSFSSRVFCSRSAEAS LKPGCWNCGAVAPSS VFLVCDSCRSIQPVDQ VDYFQI
Sbjct: 61  FLDNLSFSSRVFCSRSAEASDLKPGCWNCGAVAPSSVVFLVCDSCRSIQPVDQSVDYFQI 120

Query: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180
           FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS
Sbjct: 121 FGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLS 180

Query: 181 RAIYII 187
           RAIYI+
Sbjct: 181 RAIYIL 186

[Close]

BLAST of Cucsa.242070.2 vs. NCBI nr
Match: gi|590633148|ref|XP_007028035.1| (DNAJ heat shock N-terminal domain-containing protein, putative isoform 1 [Theobroma cacao])

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-45
Identity = 106/185 (57.30%), Postives = 125/185 (67.57%), Query Frame = 1


Query: 5   KLRSSLFYVLRTNTPLSSAI--YFPNVSNSALSSQISSISLLPSIAECSRPSPPTACEFL 64
           KL + L  V+R   P +  +  Y PN    + SS++ + S +               +FL
Sbjct: 6   KLWTPLSTVVRRALPTTCHLTPYSPNSQIRSSSSRLETSSRVSLSLHYGH-------DFL 65

Query: 65  DNLSFSSRVFCSRSA-EASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQIF 124
            NL FS++ FCS+SA +       CWNC AVA   A FL CDSCRSIQPVD  VDYFQIF
Sbjct: 66  GNLKFSAKSFCSQSAAKVDATTKRCWNCDAVAAGMAPFLYCDSCRSIQPVDFSVDYFQIF 125

Query: 125 GLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSR 184
           GLEK+YEI    LE KYKDWQKKLHPDLVHSKS++EREYAAEQSARVIDAYRTLSKPL+R
Sbjct: 126 GLEKKYEIEVDGLEGKYKDWQKKLHPDLVHSKSEKEREYAAEQSARVIDAYRTLSKPLAR 183

Query: 185 AIYII 187
           AIYI+
Sbjct: 186 AIYIL 183

[Close]

BLAST of Cucsa.242070.2 vs. NCBI nr
Match: gi|590633151|ref|XP_007028036.1| (DNAJ heat shock N-terminal domain-containing protein, putative isoform 2, partial [Theobroma cacao])

HSP 1 Score: 189.1 bits (479), Expect = 7.6e-45
Identity = 94/128 (73.44%), Postives = 104/128 (81.25%), Query Frame = 1


Query: 60  EFLDNLSFSSRVFCSRSA-EASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYF 119
           +FL NL FS++ FCS+SA +       CWNC AVA   A FL CDSCRSIQPVD  VDYF
Sbjct: 20  DFLGNLKFSAKSFCSQSAAKVDATTKRCWNCDAVAAGMAPFLYCDSCRSIQPVDFSVDYF 79

Query: 120 QIFGLEKEYEIGDVNLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKP 179
           QIFGLEK+YEI    LE KYKDWQKKLHPDLVHSKS++EREYAAEQSARVIDAYRTLSKP
Sbjct: 80  QIFGLEKKYEIEVDGLEGKYKDWQKKLHPDLVHSKSEKEREYAAEQSARVIDAYRTLSKP 139

Query: 180 LSRAIYII 187
           L+RAIYI+
Sbjct: 140 LARAIYIL 147

[Close]

BLAST of Cucsa.242070.2 vs. NCBI nr
Match: gi|359481056|ref|XP_003632561.1| (PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Vitis vinifera])

HSP 1 Score: 186.4 bits (472), Expect = 4.9e-44
Identity = 104/174 (59.77%), Postives = 129/174 (74.14%), Query Frame = 1


Query: 18  TPLSSAIY--FPNVS-NSALSSQISSISLLP--SIAECSRPSPPTACEFLDNLSFSSRVF 77
           TPLS  +   FP+ S NS L+   S+I   P  S A+ SR    ++ +FL +L FS ++ 
Sbjct: 8   TPLSPILRRRFPSFSLNSTLNIPSSTIFSSPCSSTAQYSRVFWSSSYDFLGSLKFSRKLC 67

Query: 78  CSRSAEASVLKPGCWNCGAVAPSSAVFLVCDSCRSIQPVDQLVDYFQIFGLEKEYEIGDV 137
            ++SAE   ++  CWNC A A ++  FL CDSCRS+QPVD  VDYFQIFGLEK+YE+ D 
Sbjct: 68  SAQSAEQVHVR--CWNCKAAAQTTP-FLACDSCRSVQPVDPSVDYFQIFGLEKKYELEDN 127

Query: 138 NLESKYKDWQKKLHPDLVHSKSDREREYAAEQSARVIDAYRTLSKPLSRAIYII 187
           NLE KYKDWQKKLHPDLVHSKS+ E+EYAAEQSARVIDAY+TLSKPL+RAIYI+
Sbjct: 128 NLEGKYKDWQKKLHPDLVHSKSEEEKEYAAEQSARVIDAYQTLSKPLARAIYIL 178

The following BLAST results are available for this feature:

BLAST of Cucsa.242070.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HSC20_MOUSE	5.7e-16	33.05	Iron-sulfur cluster co-chaperone protein HscB, mitochondrial OS=Mus musculus GN=...	[more]
HSCB_SERP5	3.6e-10	43.06	Co-chaperone protein HscB OS=Serratia proteamaculans (strain 568) GN=hscB PE=3 S...	[more]
HSCB_VIBCH	1.0e-09	40.28	Co-chaperone protein HscB homolog OS=Vibrio cholerae serotype O1 (strain ATCC 39...	[more]
HSCB_VIBC3	1.0e-09	40.28	Co-chaperone protein HscB homolog OS=Vibrio cholerae serotype O1 (strain ATCC 39...	[more]
HSCB_COLP3	1.4e-09	40.28	Co-chaperone protein HscB homolog OS=Colwellia psychrerythraea (strain 34H / ATC...	[more]

BLAST of Cucsa.242070.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBM6_CUCSA	5.8e-100	99.46	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G782800 PE=4 SV=1	[more]
A0A061EVT7_THECC	2.4e-45	57.30	DNAJ heat shock N-terminal domain-containing protein, putative isoform 1 OS=Theo...	[more]
A0A061EV47_THECC	5.3e-45	73.44	DNAJ heat shock N-terminal domain-containing protein, putative isoform 2 (Fragme...	[more]
D7U8Q6_VITVI	3.4e-44	59.77	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0032g00960 PE=3 SV=...	[more]
I1L9M4_SOYBN	7.7e-44	53.44	Uncharacterized protein OS=Glycine max GN=GLYMA_10G083800 PE=4 SV=2	[more]

BLAST of Cucsa.242070.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G06410.1	2.4e-41	57.24	DNAJ heat shock N-terminal domain-containing protein	[more]

BLAST of Cucsa.242070.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449437496\|ref\|XP_004136528.1\|	8.3e-100	99.46	PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Cucumis...	[more]
gi\|659084555\|ref\|XP_008442950.1\|	9.8e-93	93.55	PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Cucumis...	[more]
gi\|590633148\|ref\|XP_007028035.1\|	3.4e-45	57.30	DNAJ heat shock N-terminal domain-containing protein, putative isoform 1 [Theobr...	[more]
gi\|590633151\|ref\|XP_007028036.1\|	7.6e-45	73.44	DNAJ heat shock N-terminal domain-containing protein, putative isoform 2, partia...	[more]
gi\|359481056\|ref\|XP_003632561.1\|	4.9e-44	59.77	PREDICTED: iron-sulfur cluster co-chaperone protein HscB, mitochondrial [Vitis v...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001623	DnaJ_domain
IPR004640	HscB

Vocabulary: **Biological Process**
Term	Definition
GO:0006457	protein folding

Vocabulary: **Molecular Function**
Term	Definition
GO:0051087	chaperone binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
biological_process	GO:0051259	protein oligomerization
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051087	chaperone binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.242070	Cucsa.242070	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.242070.2	Cucsa.242070.2-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.242070.2.five_prime_UTR.1	Cucsa.242070.2.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.242070.2.CDS.1	Cucsa.242070.2.CDS.1	CDS
Cucsa.242070.2.CDS.2	Cucsa.242070.2.CDS.2	CDS
Cucsa.242070.2.CDS.3	Cucsa.242070.2.CDS.3	CDS
Cucsa.242070.2.CDS.4	Cucsa.242070.2.CDS.4	CDS
Cucsa.242070.2.CDS.5	Cucsa.242070.2.CDS.5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.242070.2.three_prime_UTR.1	Cucsa.242070.2.three_prime_UTR.1	three_prime_UTR
Cucsa.242070.2.three_prime_UTR.2	Cucsa.242070.2.three_prime_UTR.2	three_prime_UTR
Cucsa.242070.2.three_prime_UTR.3	Cucsa.242070.2.three_prime_UTR.3	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	GENE3D	G3DSA:1.10.287.110		coord: 81..185 score: 1.8
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 115..180 score: 6.
IPR001623	DnaJ domain	PROFILE	PS50076	DNAJ_2	coord: 116..188 score: 9
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 115..186 score: 8.11
IPR004640	Co-chaperone Hsc20	PANTHER	PTHR14021	FAMILY NOT NAMED	coord: 76..186 score: 5.9
None	No IPR available	PANTHER	PTHR14021:SF15	IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB, MITOCHONDRIAL	coord: 76..186 score: 5.9