Cucsa.238290.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.238290.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
Description2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Locationscaffold02023 : 907115 .. 912233 (-)
Sequence length1994
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCAACCCCTTCACAAATCGGACGCTCTGCTCTCTCTCTCCGCCCCCTAACTGACCACCGGCCGCCGTTTCTCTCCATCTCCGGCATTATTCCGGGATCGGTATCTCCTGTCTCTTAAGAAACCGATCTCTTCATAGAATCACAGCAACCACCAGTAAGTCCTTACAAATAGAATAATCGGAAGCATTTCTAAGAATTTTACTTCAATTGAGAGTATGATTAGAGAATGTTTTTGGGTCCTTTAGTTTTGTCTTACTTGATCAATTGATCGATTCAACACTCTTTTGTGAATCGTATTGTACAAGTCAGTTTGGAGATTATATGTGTAATTTTCTTTTGTTTGTTCCGCACTATCAGTGTATTCGATTCTCGAGAATCTCCTCTTTTGTCTTTGGAGTTTTTAACATTTCCTCTGTCGACATTGATGCAGAATGCAGAATAAAGTGGTAATGGCGGAAAGTGGTTGTAACATTTTATAGACCATCCATATCAGAATTTCACAATTTCGTTTTCTTTTGGGTTCAAATTCAAACCTACTTGCGAATTTAATTTATGATGGTTGTGCAGAGGCTCTAGAGGTATGGTTTGTGGAATTCCTTTGTTATCTTCATTGATCTCAATCAAATATGCATGTAAAAACGTAGAGTTTGTGAAATTGTTATGTCTTCACCTTTCTGTTATCGACGTCGAGCTTGAATTTGCTTAGAAGCAATTGTATCTGCTTCATCCGAAGAAATTTAATCTTGAAAGCTTGGATGTTTCGAAAGAAATCCATTATTATCGAGCTTCTGCGTCTATACTTCCTTTTAGGGATTTCTGGGTTGTTTCCCAATTGCCAATGCATGGATAATTGATCGAATTTAGATATTAAGTTGTATGTTAAACTATTTCTGGGTAATTGCCAGTTCACCCGGTGATTACTTTTTTTTTTTCTCTCTCTTTTTTCTCTTGAGCTAAATTGTGTATAGGACCTATTTGATAGCCATTTTTTAGTCATCTTTTGTCAAGATATAAGTTTGTTTGTTCATAATCCCTTTTATATTCTATCAACGTGTTTAGCAATTTTAAAATTTAAAATTAGTATTTTGAAAAGAAAAAAAAAATCGAAATTTGGCTAAGATTACTAAAGAGTTTTCTTGATAAAGGAGTTTAAAAGTGAAAAAAAAAAGGACAAACACGATAAGATTGCAATTAAACAAAAGTGAACTTCTCGTCGAATAAGGCTTCAATTTTCATTTGTATCTATTTTGGGTTTGATTCATGCAACTCTTTTAATTGACATCATGTTCTTAAACTTTTCTGACATATATGAAGAAGGAAAAAGTTAACTCAAATACCAAGATTGTGCAACCATGTTTATATCCCAGCAGCCAAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCAATACCAAAATTTGGATTCAAAACTCCTCTGCAAGTGGCCAAAGTTCCTAACTTGGATGCAATTGCCTCTGCTGGTGCTAATGGTCTCATGGACCCTGTCGAAGTAGGGTTGGGTTGTGGAAGTGACACAGCTCATCTTTCTCTCTTGGGCTACGACCCTAGAGTATATTATAGAGGTCGAGGTGCATTCGAGTCTATGGGAGCCGGATTGGCTATGTCACCTGGAGACATTGCATTTAAGGTGGGTTTCTCTACTTCCATTCCCTAATTTATATTCCTTCCCACCTTTTACCGTTTATCACAGCTCGGATGCATATAAGTTAGAAACATTGTAATATTTTGTTGGCTTAGGTTTTCAGTGAAAAGAAAACAGTGATTATTTGTTTAGCCGGATGTTTTCTCTTTAACTTAGAGCCTGAACTCAAATGTGTGAAGGAAATTTTATGCAAATATGATTTGTTCAGATAAAACCTATTATTTAGCATGACAATAAATGCTTTTTATTTCTCATAGTTTAATCAATGTTTAACGTCATCGTGCATTTAACTTACTCAATTTTTTGTGTAGAAAAAAGACACGTTTTTTAGGAGAATTTTGGTAACAGAATCATATAATGAAAATCCGGCCTCCATCTCTGTTTCTTTTTTTTTTTTTTTTTTGTGGAATTGGGACTTCTTTGTTTTGGAGTAGTAGTGTGCTTACGACCTTACTTGAACCCTATTTGTTCTATAGGTTGTACAACTGTGTCCTTGAGTTGTAAGAAGTAGTAGTGTGCTTCTTTCGAACTCCTTCCACGTGCCTGCAGTTTCAACTATTTTGCTCAAAACCACATCCTGTTCTTCCCACTGCTTTTATTATTTCATATTCTCCATTCTTCTTGCTATCTTCATTGGGAAATTTGGACCCCAAATTAACTTTTGGCTTCACAATTGCAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGACAGGCACTTTGAGGAGGAAGGGCCAATATTATGTGCAGCACTTGACAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTAGTTTGTACTCCTTACTTTATTAAAATCATCAAAGGTTTCATCCTGTCGATAACCATACTTTATTTGGAAGCATTTCATTATATCAACTCATTCTGGTACTAGAAAATTGAAATTTAAGCAACTGTTCTCGTTACAAAGTTGTTGGAGAAGTTCTGTAAGGGGTCTAGTTAAGAGATATATGGGGTTATCAGTAGAGTATTTGTATGATTAGTAGAAGAGGAATATTGGTAATTAGATAGTGAAGTTGGTTGGGGCTTTTTAGTTATAAATAGGAGAGGGGTTGGGCAAGGGTAAGAAGAATTTTGTGGCGAGTTCCTAATTGGTAAAATTTGGGTGAGGATTTTCAGCCCTCTAGAATGTGCTGACTGTATTGTAATTTCTTCTTGATATTGCAAATTGCAATAATATCTATATTTTAGTGTTCTTTGGTGTGTTCTTTGTGTTCTTGGGTCTTCTTGTTAGGTGGTATCCCAACAACTCCAAATGGATTCTAAGCTCGTAAATCTGAAAAAGAAAATTATCGTAATAGACCCAATGACTTCAGTGGTAAAGGGTCGTCGAGATTTTCAAATCTCACATGCCCATATTATTTGTTCACGACTTTAAATTTCAAAGGCAGATAATAAGATTTATCTCATTTTCCTTTTCCTTTTTCATTTTCTTTTTTCTTTCTCCCTGTTAGGTATGCCACAGAGCATAGGTGTGGTGTGGTTGTGAAAGGACCCAATTTGAGTGGCAATATTTCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGAGGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTGTCCAAGGAAATATCGCGAATCCTAGTCTCTCACCCAATAAATGCAAAGCGTGCAGCAGAAGGGAAAAACATAGCCAACCTCGTTCTTTTACGAGGTTGTGGTATTCGAATTGAGGTTGGTTCTTTTATATATTTCTCCTATGCTATTTGCTCTGAGTTTTCATTCATAGGATTGCAGTGTTGATGTTCATGCGTCCTTTTGCTTGGCTGCACCATGTGTTGATTCATCTCACATGAAAAGATATATTCTGCCATTGTATCTTCGACCGTTTACATTAGGTTGCAATTCAATGTGAAACTTATGCACTTGAAAAGCTTGTATAATTCGAATCTGGTAGAAAACTTGGGATCTCATTGAGGTCTTCTGTTACTGAGATGTTATCCATGCAGGAAGTGCGTTGATGAACTTCAATGAACGAACTCGTTCTCACAATTTACACTCTGCATAACAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCACCCACTAAAATTATTGCTGGTCTGGGCTTATCACTAGGAATTGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACGCTCTTAACCTCGAAAGCTACTGCAATAGCAAAGGGCCTGTCAGCTCCTTTGCAATCCAGTCCCAATGTTTTTGTGCCAGGAGAAGATGAACTCAAACCAGGTCGAGCAGATGGTTACGATTTTGGGTTCCTTCACGTTAAGGTCGGTTCACTTTTTCCTCAACTGCTAAAAAATATGTCCATTAGTGGATAAATTTTTAAACCCCCCCCCCCCCCGGATAGAAGTTCTTGTTACAGAAGCAATTGTTCTCTCTAGAACTATTTTTGGAACTTATTTTTGTAATTCCTAATGTCATATAATTATATAAAATAATGTGTGTTTAGTAATTCGTAATGTCATATAATTATATAAAATAATGTGTGTACATATATATAAACTATTCTTACAAAATTAGATACCAAAAAGATACATTTTGAAAAGTTATACCTATTCTAAGAAATTAAGGGACTAAAAAAAGGTAGTTTTGTCTTTTCTTTATTTGTAATTACACTCTAAATCTGCTCTCCTCTCACTATGTGGTTGCCTACCCATCGTGTAGGCAATAGATGATGCCGGACACGATAAGGCAACGATATTCAAAGTTAAGGGAATGGAAGCTGTCGATAGAGCAATAGGACAACTGGCAAAGCTTCTCTGGAAGGCAGAATCTAGTGGGAATTTCCAGTACTATCTATGTGTGACAGGAGACCATTCTACTCCAGTTGAGTATGGAGATCACAGTTTTGAGCCAGTTCCATTTACAATGTGTAGATTGAAAGACTTTGTAGGCGCCGTGGGTGGGGAGTCGGTAATTGATGCCATTTCTCTCGACCCATTCCCGCTCCCGACGGTCGTACCTGGTGAGGACTTGGAGTGGAGAGAAAGGCATAAGATGGAAGAAGAGAGAAACAAAGAAGGTCAAGCCTTTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTTCTGCTGCCAAGGGATGTCTTGGGCGCTTTCCTGGTGGAGAAATGATGGGTATTATTAAGAAATTCCTCAAATTGGATGCATGAATTGCTTATGTTTAAATTGTTTTTGTTTATCTCTCTCTCTTTTTAATTTGAAGACTTGCCAATAAAAATTTTGGAAGTGTTTGTTGGAAAATAAAAGAAATATAGTTCCACTTTTTTCTTCTTTTTTGGATTTGTGAATGATAGTGATTAGTTTTGTTAGGA

mRNA sequence

TCTCAACCCCTTCACAAATCGGACGCTCTGCTCTCTCTCTCCGCCCCCTAACTGACCACCGGCCGCCGTTTCTCTCCATCTCCGGCATTATTCCGGGATCGGTATCTCCTGTCTCTTAAGAAACCGATCTCTTCATAGAATCACAGCAACCACCAAATGCAGAATAAAGTGGTAATGGCGGAAAGTGGTTGTAACATTTTATAGACCATCCATATCAGAATTTCACAATTTCGTTTTCTTTTGGGTTCAAATTCAAACCTACTTGCGAATTTAATTTATGATGGTTGTGCAGAGGCTCTAGAGAAGGAAAAAGTTAACTCAAATACCAAGATTGTGCAACCATGTTTATATCCCAGCAGCCAAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCAATACCAAAATTTGGATTCAAAACTCCTCTGCAAGTGGCCAAAGTTCCTAACTTGGATGCAATTGCCTCTGCTGGTGCTAATGGTCTCATGGACCCTGTCGAAGTAGGGTTGGGTTGTGGAAGTGACACAGCTCATCTTTCTCTCTTGGGCTACGACCCTAGAGTATATTATAGAGGTCGAGGTGCATTCGAGTCTATGGGAGCCGGATTGGCTATGTCACCTGGAGACATTGCATTTAAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGACAGGCACTTTGAGGAGGAAGGGCCAATATTATGTGCAGCACTTGACAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTATGCCACAGAGCATAGGTGTGGTGTGGTTGTGAAAGGACCCAATTTGAGTGGCAATATTTCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGAGGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTGTCCAAGGAAATATCGCGAATCCTAGTCTCTCACCCAATAAATGCAAAGCGTGCAGCAGAAGGGAAAAACATAGCCAACCTCGTTCTTTTACGAGGTTGTGGTATTCGAATTGAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCACCCACTAAAATTATTGCTGGTCTGGGCTTATCACTAGGAATTGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACGCTCTTAACCTCGAAAGCTACTGCAATAGCAAAGGGCCTGTCAGCTCCTTTGCAATCCAGTCCCAATGTTTTTGTGCCAGGAGAAGATGAACTCAAACCAGGTCGAGCAGATGGTTACGATTTTGGGTTCCTTCACGTTAAGGCAATAGATGATGCCGGACACGATAAGGCAACGATATTCAAAGTTAAGGGAATGGAAGCTGTCGATAGAGCAATAGGACAACTGGCAAAGCTTCTCTGGAAGGCAGAATCTAGTGGGAATTTCCAGTACTATCTATGTGTGACAGGAGACCATTCTACTCCAGTTGAGTATGGAGATCACAGTTTTGAGCCAGTTCCATTTACAATGTGTAGATTGAAAGACTTTGTAGGCGCCGTGGGTGGGGAGTCGGTAATTGATGCCATTTCTCTCGACCCATTCCCGCTCCCGACGGTCGTACCTGGTGAGGACTTGGAGTGGAGAGAAAGGCATAAGATGGAAGAAGAGAGAAACAAAGAAGGTCAAGCCTTTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTTCTGCTGCCAAGGGATGTCTTGGGCGCTTTCCTGGTGGAGAAATGATGGGTATTATTAAGAAATTCCTCAAATTGGATGCATGAATTGCTTATGTTTAAATTGTTTTTGTTTATCTCTCTCTCTTTTTAATTTGAAGACTTGCCAATAAAAATTTTGGAAGTGTTTGTTGGAAAATAAAAGAAATATAGTTCCACTTTTTTCTTCTTTTTTGGATTTGTGAATGATAGTGATTAGTTTTGTTAGGA

Coding sequence (CDS)

ATGTTTATATCCCAGCAGCCAAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCAATACCAAAATTTGGATTCAAAACTCCTCTGCAAGTGGCCAAAGTTCCTAACTTGGATGCAATTGCCTCTGCTGGTGCTAATGGTCTCATGGACCCTGTCGAAGTAGGGTTGGGTTGTGGAAGTGACACAGCTCATCTTTCTCTCTTGGGCTACGACCCTAGAGTATATTATAGAGGTCGAGGTGCATTCGAGTCTATGGGAGCCGGATTGGCTATGTCACCTGGAGACATTGCATTTAAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGACAGGCACTTTGAGGAGGAAGGGCCAATATTATGTGCAGCACTTGACAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTATGCCACAGAGCATAGGTGTGGTGTGGTTGTGAAAGGACCCAATTTGAGTGGCAATATTTCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGAGGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTGTCCAAGGAAATATCGCGAATCCTAGTCTCTCACCCAATAAATGCAAAGCGTGCAGCAGAAGGGAAAAACATAGCCAACCTCGTTCTTTTACGAGGTTGTGGTATTCGAATTGAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCACCCACTAAAATTATTGCTGGTCTGGGCTTATCACTAGGAATTGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACGCTCTTAACCTCGAAAGCTACTGCAATAGCAAAGGGCCTGTCAGCTCCTTTGCAATCCAGTCCCAATGTTTTTGTGCCAGGAGAAGATGAACTCAAACCAGGTCGAGCAGATGGTTACGATTTTGGGTTCCTTCACGTTAAGGCAATAGATGATGCCGGACACGATAAGGCAACGATATTCAAAGTTAAGGGAATGGAAGCTGTCGATAGAGCAATAGGACAACTGGCAAAGCTTCTCTGGAAGGCAGAATCTAGTGGGAATTTCCAGTACTATCTATGTGTGACAGGAGACCATTCTACTCCAGTTGAGTATGGAGATCACAGTTTTGAGCCAGTTCCATTTACAATGTGTAGATTGAAAGACTTTGTAGGCGCCGTGGGTGGGGAGTCGGTAATTGATGCCATTTCTCTCGACCCATTCCCGCTCCCGACGGTCGTACCTGGTGAGGACTTGGAGTGGAGAGAAAGGCATAAGATGGAAGAAGAGAGAAACAAAGAAGGTCAAGCCTTTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTTCTGCTGCCAAGGGATGTCTTGGGCGCTTTCCTGGTGGAGAAATGATGGGTATTATTAAGAAATTCCTCAAATTGGATGCATGA

Protein sequence

MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMGIIKKFLKLDA*
BLAST of Cucsa.238290.1 vs. Swiss-Prot
Match: APGM_METS5 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=apgM PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.5e-67
Identity = 152/397 (38.29%), Postives = 223/397 (56.17%), Query Frame = 1

Query: 8   KKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTA 67
           K ++  V+ DGLGD  +     +TPL+ A  P + ++  +   GLMDP+  G+  GSDT+
Sbjct: 3   KYKILLVIGDGLGDRQVASLNGRTPLENADKPTIASLLRSSLVGLMDPIGPGIVPGSDTS 62

Query: 68  HLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEE 127
           HL++ G DP+ YY+GRG+FE++GAG  ++ GDIAF+ NFAT+D    +V  RRA R  EE
Sbjct: 63  HLAIFGLDPKKYYKGRGSFEALGAGAILTEGDIAFRGNFATVDSNL-VVIDRRAGRKIEE 122

Query: 128 EGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQA 187
              ++    D  KI      +VR  + TEHR  VV+ G NLS  +S TDP +  + +L +
Sbjct: 123 AEDLVKELND--KIQEIDGVKVRFYHGTEHRVSVVLSGDNLSDKVSDTDPHEVGKRILNS 182

Query: 188 EALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPP 247
           E  DD+  +K TA ++N L++ I  +L +  +N KR  EG   AN+VLLRG  I  E+P 
Sbjct: 183 EPTDDALSSKRTANIINALTRRIYEVLSNSQLNDKRVREGLPPANIVLLRGASIHTELPK 242

Query: 248 FEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQS 307
            +   GL    V+ T +I G+  SLG++++  PGATG   T   +KA A AK L      
Sbjct: 243 LKDYTGLSGAAVSATALIKGVCKSLGMEVVTPPGATGGIDTDYMAKAEAAAKLL------ 302

Query: 308 SPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLL 367
                            + +D  FLH+KA D A HD     KVK +E +DR+IG++    
Sbjct: 303 -----------------EDHDLVFLHIKATDAASHDGKVSEKVKAIEMIDRSIGRV---- 362

Query: 368 WKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTM 405
              +  G+ +  +  TGDH+TPVE  +HS +PVP  +
Sbjct: 363 --LDRYGS-ELVVLFTGDHATPVELREHSGDPVPLML 366

BLAST of Cucsa.238290.1 vs. Swiss-Prot
Match: APGM1_METTH (2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) GN=apgM1 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.0e-67
Identity = 148/391 (37.85%), Postives = 212/391 (54.22%), Query Frame = 1

Query: 14  VLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTAHLSLLG 73
           ++IDG+ D  + K   +TPL+ A  PN+D IA +G NG+MDP++ G+  GSDT+HLS+LG
Sbjct: 6   MIIDGMADRPLEKLSGRTPLEAASTPNMDRIAESGINGIMDPIKPGVRAGSDTSHLSILG 65

Query: 74  YDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEGPILC 133
           YDP   Y GRG FE+ G GL + PGDIAF+ NFAT DE   I+T RRA R        + 
Sbjct: 66  YDPYRVYTGRGPFEAAGVGLDVKPGDIAFRCNFATADEDM-IITDRRAGR-IRSGTSSIA 125

Query: 134 AALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEALDDS 193
             ++ M I  F + E+  R +T HR  +V++GP L   +S  DP  + +   + +ALD S
Sbjct: 126 RTINSMTIEGFEDVEIIFRESTGHRAVLVLRGPGLGDKVSDADPKVEGKPPKKIKALDGS 185

Query: 194 EEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPPFEKKHG 253
            E++ TA V+N + KE   IL  HP+N  R   G+  AN++L RG G    V  F++++ 
Sbjct: 186 PESRKTAEVLNRIVKESYEILRDHPVNTGRIERGEAPANIILPRGAGAVPHVEKFQERYD 245

Query: 254 LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQSSPNVFV 313
           L    +A T +I G+G   G+D++D  GATG   T L S   AI+  ++A          
Sbjct: 246 LKAACIAETGLIKGIGHLTGMDVIDVEGATGGIDTDLESIKNAISSAIAA---------- 305

Query: 314 PGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWKAESS 373
                        YDF  +++   D+AGHD     KV+ +E VD  +G L          
Sbjct: 306 ------------DYDFLLINIDGADEAGHDGDLEGKVRFIERVDSILGDLM--------- 362

Query: 374 GNFQYYLCVTGDHSTPVEYGDHSFEPVPFTM 405
                Y  +T DHSTPV   DH+ +PVP  +
Sbjct: 366 -GDDIYFILTADHSTPVSVMDHTGDPVPIAI 362

BLAST of Cucsa.238290.1 vs. Swiss-Prot
Match: APGM_IGNH4 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) GN=apgM PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.9e-66
Identity = 158/441 (35.83%), Postives = 232/441 (52.61%), Query Frame = 1

Query: 9   KRVAFVLI--DGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDT 68
           K++  VLI  DG+GD  +P  G KTPL+VA  PNLD  A  G  GLMD +  G+  GSDT
Sbjct: 2   KQLKMVLIVGDGMGDRLVPSLGNKTPLEVASTPNLDEAARRGQAGLMDVIAPGVPPGSDT 61

Query: 69  AHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTG--IVTSRRADRH 128
           AHL+L G DP  +Y GRG FE++G G  + PGD+A + NFAT++E+ G  +V  RRA R+
Sbjct: 62  AHLALFGLDPFEWYEGRGPFEALGVGAEVGPGDVALRGNFATVEERGGRLVVVDRRAGRY 121

Query: 129 FEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 188
             E   ++ A  +  ++      +VR  +ATEHR  VV+KG  LS  +S TDP +  + +
Sbjct: 122 LPEAEELVKALNE--ELSEVEGVKVRFYHATEHRVAVVLKGEGLSDEVSDTDPHEVGKPV 181

Query: 189 LQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG-IRI 248
            +A  L D+ EAK TA V+NE++    +IL  HP N +R  +G   AN+VLLRG G +R 
Sbjct: 182 QEARPLRDTPEAKKTARVINEITFRSYQILKDHPANKRRVEKGLPPANIVLLRGAGMMRK 241

Query: 249 EVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSA 308
           ++P  ++++G+    V  T ++ G+  ++G+D++  PGATG   T   SKA    + L  
Sbjct: 242 KLPTLQERYGIKAAAVGATALVLGVARAVGMDVIVPPGATGGVNTDYKSKARTAVELLK- 301

Query: 309 PLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQL 368
                                  YD  F+H+K  D A HD     K+K +EA+D  +G L
Sbjct: 302 ----------------------DYDMVFVHIKGTDAASHDGDVENKIKMIEALDYVLGYL 361

Query: 369 AKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAI 428
                      + +    VT DH+TPV   +H+ +PVP  +                   
Sbjct: 362 LDYY-------DGEAVFAVTPDHATPVTVKEHTGDPVPVLLY------------------ 386

Query: 429 SLDPFPLPTVVPGEDLEWRER 445
                  PT++P E +E+ ER
Sbjct: 422 ------APTLIPDEAVEYNER 386

BLAST of Cucsa.238290.1 vs. Swiss-Prot
Match: APGM_SULSO (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=apgM PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.5e-65
Identity = 148/392 (37.76%), Postives = 214/392 (54.59%), Query Frame = 1

Query: 10  RVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTAHL 69
           ++  ++ DGLGD  + K    TPL+ A  P +  +      GLMDP+  G+  GSDT+HL
Sbjct: 5   KILLIVADGLGDRPVSKLNGLTPLEAANKPAITDLLKNSMIGLMDPISPGIIPGSDTSHL 64

Query: 70  SLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEG 129
           S+ G DP VYYRGRGAFE++GAG  +S GD+AF+ NFAT++    +V  RRA R   EEG
Sbjct: 65  SIFGLDPHVYYRGRGAFEALGAGATLSHGDVAFRGNFATVNNDL-VVVDRRAGRKL-EEG 124

Query: 130 PILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEA 189
             L   L+  KI    + +++    TEHR  VV+ G  +S  +S TDP  +   +L+++ 
Sbjct: 125 EQLVKELNE-KIKEINDVKIKFYKGTEHRIAVVLSGKGISDKVSDTDPHHEGLKVLESKP 184

Query: 190 LDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPPFE 249
           L+DS EA  TA ++N L++++  +L S  IN +R  +G+  AN+VLLRG    +++PPF 
Sbjct: 185 LEDSNEAIRTAEIINILTRKVFDVLNSSEINKRRIEQGEKPANIVLLRGAAHYVKLPPFS 244

Query: 250 KKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQSSP 309
               L    V+ T +I G+   LG++++   GATG   T   +KA A  + L        
Sbjct: 245 SYTKLKAAAVSATALIKGICRELGMNVITPSGATGGIDTNYNAKAKAAIELLKE------ 304

Query: 310 NVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWK 369
                             DF FLH+KA D A HD     KVK +E +DR IG +      
Sbjct: 305 -----------------NDFVFLHIKATDAASHDGLVEEKVKAIERIDRVIGAI------ 364

Query: 370 AESSGNFQYYLCVTGDHSTPVEYGDHSFEPVP 402
            ++ G     L  TGDH TPVE  +H+ +PVP
Sbjct: 365 IDNIGRDNLILMFTGDHVTPVEIKEHTGDPVP 364

BLAST of Cucsa.238290.1 vs. Swiss-Prot
Match: APGM_SULTO (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) GN=apgM PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.2e-65
Identity = 148/405 (36.54%), Postives = 220/405 (54.32%), Query Frame = 1

Query: 10  RVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTAHL 69
           ++ F + DGLGD  + K   KTPL+    PN+  +      GLMDP+  G+  GSDT+HL
Sbjct: 5   KILFFIADGLGDRPVRKLQGKTPLEYVDKPNIRELLKNSIIGLMDPISPGVVAGSDTSHL 64

Query: 70  SLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEG 129
           S+ G DP  YYRGRGAFE++GAG  +   D+AF+ NFAT++ +  IV  RRA R  EE  
Sbjct: 65  SMFGLDPHKYYRGRGAFEAIGAGARLKASDVAFRGNFATVNNEF-IVVDRRAGRKIEEAD 124

Query: 130 PILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEA 189
            ++    +  KI      +VR  + TEHR  VV+ G  L+  +S TDP + N+ +L+++ 
Sbjct: 125 DLVKELNE--KIGEIDGVKVRFYHGTEHRVAVVLSGKGLTDKVSDTDPHEVNKKVLESKP 184

Query: 190 LDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPPFE 249
           LD+S E++ TA ++N+L+++I  IL S  IN  R ++G+  AN++LLRG    +E+P FE
Sbjct: 185 LDNSPESQFTANIINKLTRKIYEILNSSEINKIRVSKGELPANIILLRGAAEFVELPQFE 244

Query: 250 KKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQSSP 309
               L    V+ T +I G+   +G+ ++  PGATG   T    KA A  + L        
Sbjct: 245 SYTKLKAAAVSATALIKGICEQIGMRVVTPPGATGGLDTNYLGKADAAVELLKE------ 304

Query: 310 NVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWK 369
                            YDF FLH+KA D A HD     KV  +  +D  IG++   L  
Sbjct: 305 -----------------YDFVFLHLKATDAASHDGNVDGKVYAINKMDEMIGRILDKL-- 364

Query: 370 AESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAV 415
                  +  + ++GDH+TPVE  +H+ +PVPF +    D +  V
Sbjct: 365 -----GSELVIAISGDHTTPVEVKEHTGDPVPFLLYVPYDIINDV 376

BLAST of Cucsa.238290.1 vs. TrEMBL
Match: A0A0A0KSM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643310 PE=4 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 3.0e-288
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 1

Query: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60
           MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL
Sbjct: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240
           NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420
           GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI
Sbjct: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420

Query: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480
           DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP
Sbjct: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKKFLKLDA 497
           GGEMMGIIKKFLKLDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

BLAST of Cucsa.238290.1 vs. TrEMBL
Match: A0A067F5X4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011087mg PE=4 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 3.1e-248
Identity = 423/490 (86.33%), Postives = 460/490 (93.88%), Query Frame = 1

Query: 7   PKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDT 66
           PK+RVAFVLIDGLGDVS+P+FG+KTPLQ A VPNLDAIASAG NGL+DPVEVGLGCGSDT
Sbjct: 7   PKRRVAFVLIDGLGDVSLPRFGYKTPLQAAYVPNLDAIASAGVNGLLDPVEVGLGCGSDT 66

Query: 67  AHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126
           AHLSLLGY+PRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE
Sbjct: 67  AHLSLLGYNPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126

Query: 127 EEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186
           EEGPILCAALDRMK+PSFP+YEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL Q
Sbjct: 127 EEGPILCAALDRMKLPSFPQYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLFQ 186

Query: 187 AEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVP 246
           A+ALDD++EAK TAAVVNELS+EIS+ILVSHP+NAKRAAEGKNIAN+VLLRGCGIRIEVP
Sbjct: 187 AQALDDTDEAKHTAAVVNELSREISKILVSHPLNAKRAAEGKNIANVVLLRGCGIRIEVP 246

Query: 247 PFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQ 306
            FEKKHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRT+LTSKATAIAK LSAPLQ
Sbjct: 247 SFEKKHGLWPCMVAPTKIIAGLGLSLDIDILEAPGATGDYRTILTSKATAIAKALSAPLQ 306

Query: 307 SSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKL 366
           S PNVFVPGEDE KPGR+DGYDFGFLH+KA+DDAGHDKA+IFKVK +EAVDRAIGQLA+L
Sbjct: 307 SCPNVFVPGEDEHKPGRSDGYDFGFLHIKAVDDAGHDKASIFKVKALEAVDRAIGQLARL 366

Query: 367 LWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLD 426
           LW+AE SG FQY+LCVTGDHSTPVEYGDHSFEPVP  MC+LKDFVGAVGGES +  I LD
Sbjct: 367 LWQAEKSGKFQYFLCVTGDHSTPVEYGDHSFEPVPLAMCQLKDFVGAVGGESAVMEIPLD 426

Query: 427 PFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMG 486
           PFPLPTV  GEDL   E  K+E+ER+++ QAF GDSVCEF+E++AA+GCLGRFPGGEMMG
Sbjct: 427 PFPLPTVKAGEDL--TEDKKIEKERSEQLQAFSGDSVCEFNEVAAARGCLGRFPGGEMMG 486

Query: 487 IIKKFLKLDA 497
           IIK +LKL A
Sbjct: 487 IIKTYLKLKA 494

BLAST of Cucsa.238290.1 vs. TrEMBL
Match: V4UWH8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011558mg PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 4.0e-248
Identity = 424/490 (86.53%), Postives = 460/490 (93.88%), Query Frame = 1

Query: 7   PKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDT 66
           PK+RVAFVLIDGLGDVS+P+FG+KTPLQ A VPNLDAIASAG NGLMDPVEVGLGCGSDT
Sbjct: 7   PKRRVAFVLIDGLGDVSLPRFGYKTPLQAAYVPNLDAIASAGVNGLMDPVEVGLGCGSDT 66

Query: 67  AHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126
           AHLSLLGY+PRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE
Sbjct: 67  AHLSLLGYNPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126

Query: 127 EEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186
           EEGPILCAALDRMK+PSFP+YEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ
Sbjct: 127 EEGPILCAALDRMKLPSFPQYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186

Query: 187 AEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVP 246
           A+ALDD++EAK TAAVVNELS+EIS+ILVSHP+NAKRAAEGKNIAN+VLLRGCGIRIEVP
Sbjct: 187 AQALDDTDEAKHTAAVVNELSREISKILVSHPLNAKRAAEGKNIANVVLLRGCGIRIEVP 246

Query: 247 PFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQ 306
            FEKKHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRT+LTSKATAIAK LSAPLQ
Sbjct: 247 SFEKKHGLWPCMVAPTKIIAGLGLSLDIDILEAPGATGDYRTILTSKATAIAKALSAPLQ 306

Query: 307 SSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKL 366
           S PNVFVPGEDE KPGR+DGYDFGFLH+KA+DDAGHDKA+IFKVK +EAVDRAIGQLA+L
Sbjct: 307 SCPNVFVPGEDEHKPGRSDGYDFGFLHIKAVDDAGHDKASIFKVKALEAVDRAIGQLARL 366

Query: 367 LWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLD 426
           LW+AE SG FQY+LCVTGDHSTPVEYGDHSFEPVP  MC+LKDFVGAVGGES +  I  D
Sbjct: 367 LWQAEKSGKFQYFLCVTGDHSTPVEYGDHSFEPVPLAMCQLKDFVGAVGGESAVMEIPPD 426

Query: 427 PFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMG 486
           PFPLPTV  GEDL   E  K+E+ER+++ QAF GDSVCEF+E++AA+GCLGRFPGGEMMG
Sbjct: 427 PFPLPTVKAGEDL--TEDTKIEKERSEQLQAFSGDSVCEFNEVAAARGCLGRFPGGEMMG 486

Query: 487 IIKKFLKLDA 497
           IIK +LKL A
Sbjct: 487 IIKTYLKLKA 494

BLAST of Cucsa.238290.1 vs. TrEMBL
Match: A0A061F8H4_THECC (Cofactor-independent phosphoglycerate mutase OS=Theobroma cacao GN=TCM_031895 PE=4 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 1.3e-246
Identity = 425/493 (86.21%), Postives = 462/493 (93.71%), Query Frame = 1

Query: 5   QQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGS 64
           QQPKKRVAFVLIDGLGDVSIP+FG+KTPLQ A +PN+DAIASAG NGLMDPVEVGLGCGS
Sbjct: 5   QQPKKRVAFVLIDGLGDVSIPRFGYKTPLQAANIPNMDAIASAGVNGLMDPVEVGLGCGS 64

Query: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH 124
           DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH
Sbjct: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH 124

Query: 125 FEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 184
           FEEEGPILCAALDRMK+PSFPEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLL
Sbjct: 125 FEEEGPILCAALDRMKLPSFPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLL 184

Query: 185 LQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIE 244
           L+A+ALDD++EA+ TAAVVNELSKEISRILVSHP+NAKR AEGKNIAN+VLLRGCGIRIE
Sbjct: 185 LEAKALDDTDEARHTAAVVNELSKEISRILVSHPLNAKRLAEGKNIANVVLLRGCGIRIE 244

Query: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAP 304
           VPPFEKKHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRTLLTSKATAIAK LSAP
Sbjct: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLHIDILEAPGATGDYRTLLTSKATAIAKALSAP 304

Query: 305 LQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLA 364
           LQS P+VFVPGEDE KPGR+ GYDFGFLH+KAIDDAGHDKA++FKVKG+EAVD+AIGQLA
Sbjct: 305 LQSCPSVFVPGEDEHKPGRSYGYDFGFLHIKAIDDAGHDKASVFKVKGLEAVDQAIGQLA 364

Query: 365 KLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAIS 424
           KLLW+AES+GNFQY++CVTGDHSTPVEYGDHSFEPVPFTMCRLKD+VGA+GGES++   S
Sbjct: 365 KLLWQAESTGNFQYFVCVTGDHSTPVEYGDHSFEPVPFTMCRLKDYVGAIGGESIVLETS 424

Query: 425 LDPFPLPTVVPGEDLEWRERHKMEEERN-KEGQAFGGDSVCEFDEISAAKGCLGRFPGGE 484
           LDPFPLPTV  GEDL   E   +E+ R  K+ Q F GDSV E +EI+AA+GCLGRFPGG+
Sbjct: 425 LDPFPLPTVKAGEDL--NEDIGLEKGRRCKQVQGFCGDSVFELNEIAAARGCLGRFPGGQ 484

Query: 485 MMGIIKKFLKLDA 497
           MM IIK+FLKL+A
Sbjct: 485 MMEIIKRFLKLNA 495

BLAST of Cucsa.238290.1 vs. TrEMBL
Match: M5WS34_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004729mg PE=4 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 1.3e-246
Identity = 429/493 (87.02%), Postives = 462/493 (93.71%), Query Frame = 1

Query: 5   QQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGS 64
           QQPK+RVAF+LIDG+GDVSIPKFGFKTPLQ AKVPNLDAIASAG NGLMDPVEVGLGCGS
Sbjct: 5   QQPKRRVAFLLIDGVGDVSIPKFGFKTPLQAAKVPNLDAIASAGVNGLMDPVEVGLGCGS 64

Query: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH 124
           DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIV +RRADRH
Sbjct: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVINRRADRH 124

Query: 125 FEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 184
           FEEEGPILCAALD MK+PSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL
Sbjct: 125 FEEEGPILCAALDGMKLPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 184

Query: 185 LQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIE 244
           LQAEALD ++EA+ TA VVNELSKEIS ILV+HP+NAKRAAEGKNIANLVLLRGCGIRIE
Sbjct: 185 LQAEALDGTDEARHTAKVVNELSKEISHILVAHPLNAKRAAEGKNIANLVLLRGCGIRIE 244

Query: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAP 304
           VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDIL+APGATGDYRTLLTSKATAIAK LSAP
Sbjct: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILEAPGATGDYRTLLTSKATAIAKALSAP 304

Query: 305 LQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLA 364
           LQS PNVFVPGEDE KPGR+DGYDFGFLHVKAIDDAGHDKATIFKVK +EAVDRAIGQL+
Sbjct: 305 LQSCPNVFVPGEDEHKPGRSDGYDFGFLHVKAIDDAGHDKATIFKVKALEAVDRAIGQLS 364

Query: 365 KLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAIS 424
           +LLW++ES+GNF+Y+LCVTGDHSTPVEYGDHSFEPVPFT+C LKDFV AVG E+++   S
Sbjct: 365 RLLWESESAGNFKYFLCVTGDHSTPVEYGDHSFEPVPFTICPLKDFVDAVGVETILGG-S 424

Query: 425 LDPFPLPTVVPGEDLEWRERHKMEE-ERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGE 484
           LDPFPLPTV  GE L   E  K+E+ ER+K+ +AF GDSV EF+EI+AA+GCLGRFPGGE
Sbjct: 425 LDPFPLPTVKDGEHL--AEDVKIEQGERSKQPRAFRGDSVYEFNEIAAARGCLGRFPGGE 484

Query: 485 MMGIIKKFLKLDA 497
           MMG+IK FLKLDA
Sbjct: 485 MMGVIKNFLKLDA 494

BLAST of Cucsa.238290.1 vs. TAIR10
Match: AT3G30841.1 (AT3G30841.1 Cofactor-independent phosphoglycerate mutase)

HSP 1 Score: 812.4 bits (2097), Expect = 1.6e-235
Identity = 401/489 (82.00%), Postives = 440/489 (89.98%), Query Frame = 1

Query: 8   KKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDTA 67
           KKRVAFVLIDGLGDV+IPK G+KTPLQ A +PNLDAIASAG NGLMDPVEVGLGCGSDTA
Sbjct: 9   KKRVAFVLIDGLGDVTIPKLGYKTPLQAANIPNLDAIASAGINGLMDPVEVGLGCGSDTA 68

Query: 68  HLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEE 127
           HLSL+GYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEK+G+V SRRADRHFEE
Sbjct: 69  HLSLMGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKSGVVVSRRADRHFEE 128

Query: 128 EGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQA 187
           EGPILCAALD +K+ SFPEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLLL+A
Sbjct: 129 EGPILCAALDGLKLQSFPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLLLEA 188

Query: 188 EALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVPP 247
           + LD+SEEA  TA VVNELS+EISRILVSHP+NA+RAA+GKNIANLVLLRGCGIRIEVPP
Sbjct: 189 KPLDESEEATHTAKVVNELSREISRILVSHPVNAERAAQGKNIANLVLLRGCGIRIEVPP 248

Query: 248 FEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQS 307
           F +KHGLWPCMVAPTKIIAGLG+SLGIDIL+APGATGDYRTLLTSKATAIA  LSAPL  
Sbjct: 249 FHEKHGLWPCMVAPTKIIAGLGMSLGIDILEAPGATGDYRTLLTSKATAIANALSAPLNP 308

Query: 308 SPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLL 367
            PNVFVPGED  KPGR+DGYDFGFLH+KAIDDAGHDKAT+FKV+G+EAVD+AI QLAKLL
Sbjct: 309 CPNVFVPGEDGHKPGRSDGYDFGFLHIKAIDDAGHDKATMFKVRGLEAVDKAIRQLAKLL 368

Query: 368 WKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLDP 427
           W+AESS ++QY+LCVTGDHSTPVEYGDHSFEPVPFTMCRL+DFV AVGGES +   SLDP
Sbjct: 369 WQAESSTHYQYFLCVTGDHSTPVEYGDHSFEPVPFTMCRLRDFVSAVGGESAVLETSLDP 428

Query: 428 FPLPTVV-PGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMG 487
           FPLPTVV  GED+  +E    E  R +   A GGD V E +E++AA+GCLGRF GGEMMG
Sbjct: 429 FPLPTVVESGEDVTKQEE---ESGRREAPLAIGGDLVAELNEVAAARGCLGRFHGGEMMG 488

Query: 488 IIKKFLKLD 496
           +I  FL L+
Sbjct: 489 VITNFLNLE 494

BLAST of Cucsa.238290.1 vs. TAIR10
Match: AT4G09520.1 (AT4G09520.1 Cofactor-independent phosphoglycerate mutase)

HSP 1 Score: 801.2 bits (2068), Expect = 3.6e-232
Identity = 398/494 (80.57%), Postives = 439/494 (88.87%), Query Frame = 1

Query: 5   QQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGS 64
           ++ +KRVAF+LIDGLGDVSIP+ G+KTPLQ A++PNLDAIASAG NGLMDPVEVGLGCGS
Sbjct: 2   EEVQKRVAFILIDGLGDVSIPRLGYKTPLQAAEIPNLDAIASAGINGLMDPVEVGLGCGS 61

Query: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH 124
           DTAHLSL+GYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDE +G+V SRRADRH
Sbjct: 62  DTAHLSLMGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEDSGVVVSRRADRH 121

Query: 125 FEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 184
           FEEEGPILCAALD MK+PSFPEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLL
Sbjct: 122 FEEEGPILCAALDGMKLPSFPEYEVRVRYATEHRCGVVVKGPKLSGNISGTDPLKDNRLL 181

Query: 185 LQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIE 244
           L+A+ LD+SEEA  TA VVNELS+EISRILVSHP+N KRAA+GKNIANLVLLRGCGIRIE
Sbjct: 182 LEAKPLDESEEANHTAKVVNELSREISRILVSHPVNVKRAAQGKNIANLVLLRGCGIRIE 241

Query: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAP 304
           VPPF +KHGLWPCMVAPTKIIAGLG+SLGIDIL+A GATGDYRTLLTSKA AIA  LSAP
Sbjct: 242 VPPFHEKHGLWPCMVAPTKIIAGLGMSLGIDILEALGATGDYRTLLTSKAIAIANALSAP 301

Query: 305 LQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLA 364
           L   PNVF P ED  KPGR+DGYDFGFLH+KAIDDAGHDKAT+FKV+G+EAVD+AI QLA
Sbjct: 302 LNPCPNVFEPAEDGHKPGRSDGYDFGFLHIKAIDDAGHDKATLFKVRGLEAVDKAIRQLA 361

Query: 365 KLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAIS 424
           KLLW+AESS N+QY+LCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFV AVGGES +   S
Sbjct: 362 KLLWQAESSSNYQYFLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVRAVGGESALLETS 421

Query: 425 LDPFPLPT-VVPGEDLEWRERHKMEEE--RNKEGQAFGGDSVCEFDEISAAKGCLGRFPG 484
           LDPFPLPT +   E++  +E    EE+  R +   A  GDSV E +EI+AA+ CLGRF G
Sbjct: 422 LDPFPLPTDIASSENVIGKE----EEDCGRREASLAISGDSVSELNEIAAARRCLGRFHG 481

Query: 485 GEMMGIIKKFLKLD 496
           GEMMG+I KFLKLD
Sbjct: 482 GEMMGVITKFLKLD 491

BLAST of Cucsa.238290.1 vs. NCBI nr
Match: gi|778707549|ref|XP_011656023.1| (PREDICTED: uncharacterized protein LOC101219404 [Cucumis sativus])

HSP 1 Score: 998.4 bits (2580), Expect = 4.4e-288
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 1

Query: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60
           MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL
Sbjct: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240
           NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420
           GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI
Sbjct: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420

Query: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480
           DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP
Sbjct: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKKFLKLDA 497
           GGEMMGIIKKFLKLDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

BLAST of Cucsa.238290.1 vs. NCBI nr
Match: gi|659119038|ref|XP_008459441.1| (PREDICTED: uncharacterized protein LOC103498576 [Cucumis melo])

HSP 1 Score: 960.3 bits (2481), Expect = 1.3e-276
Identity = 480/496 (96.77%), Postives = 482/496 (97.18%), Query Frame = 1

Query: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60
           M ISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAG NGLMDPVEVGL
Sbjct: 1   MVISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIV+SRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVSSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240
           NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRT LTSKATAIAKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTRLTSKATAIAKG 300

Query: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPL S PNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLHSCPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420
           GQLAKLLWKAE SGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKD VGAVGGESVI
Sbjct: 361 GQLAKLLWKAECSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDLVGAVGGESVI 420

Query: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480
           D ISLDPFPLPTVVPGEDLEWRE HKMEEERN+E QAFGGDSVCEFDEISAAKGCLGRFP
Sbjct: 421 DGISLDPFPLPTVVPGEDLEWRESHKMEEERNQECQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKKFLKLDA 497
           GGEMM    KFLKLDA
Sbjct: 481 GGEMM----KFLKLDA 492

BLAST of Cucsa.238290.1 vs. NCBI nr
Match: gi|1000961179|ref|XP_015576042.1| (PREDICTED: LOW QUALITY PROTEIN: probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Ricinus communis])

HSP 1 Score: 867.5 bits (2240), Expect = 1.2e-248
Identity = 428/491 (87.17%), Postives = 460/491 (93.69%), Query Frame = 1

Query: 6   QPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSD 65
           QPK+RVAFVLIDGLGDVS+P+FG KTPLQ A VPNLDAIAS+G NGLMDPVEVGLGCGSD
Sbjct: 6   QPKRRVAFVLIDGLGDVSLPRFGSKTPLQTANVPNLDAIASSGVNGLMDPVEVGLGCGSD 65

Query: 66  TAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHF 125
           TAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFAT DEKTGIVTSRRADRHF
Sbjct: 66  TAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATFDEKTGIVTSRRADRHF 125

Query: 126 EEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLL 185
           EEEGPILCAALDRMK+PS+PEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLLL
Sbjct: 126 EEEGPILCAALDRMKLPSYPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLLL 185

Query: 186 QAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEV 245
           QAEAL D++EA+ TAAVVNELS+E+SRIL+SHP+NAKRAAEGKNIAN+VLLRGCGIRIEV
Sbjct: 186 QAEALADTDEARHTAAVVNELSREMSRILLSHPLNAKRAAEGKNIANVVLLRGCGIRIEV 245

Query: 246 PPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPL 305
           P FE+KHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRTLLTSKA+AIAK LSAPL
Sbjct: 246 PSFERKHGLWPCMVAPTKIIAGLGLSLDIDILEAPGATGDYRTLLTSKASAIAKALSAPL 305

Query: 306 QSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAK 365
           QS PNVFVPGEDELKPGRADGYDFGFLH+KAIDDAGHDKA+IFKVKG+EAVDRAIGQLAK
Sbjct: 306 QSCPNVFVPGEDELKPGRADGYDFGFLHIKAIDDAGHDKASIFKVKGLEAVDRAIGQLAK 365

Query: 366 LLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISL 425
           LLW+AES+GNFQY+LCVTGDHSTPVEYGDHSFEPVPF MCRL DFVGAVGGES I A SL
Sbjct: 366 LLWQAESTGNFQYFLCVTGDHSTPVEYGDHSFEPVPFAMCRLTDFVGAVGGESNIVATSL 425

Query: 426 DPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMM 485
           +PFPLPT+  GEDLE  E    +E  N+E +AFGGDSVCEF EI+AA+GCLGRF GGEMM
Sbjct: 426 EPFPLPTIKAGEDLE--EDKMPKESGNQELKAFGGDSVCEFSEIAAARGCLGRFQGGEMM 485

Query: 486 GIIKKFLKLDA 497
           G+IK FLKL+A
Sbjct: 486 GVIKAFLKLNA 494

BLAST of Cucsa.238290.1 vs. NCBI nr
Match: gi|641839969|gb|KDO58892.1| (hypothetical protein CISIN_1g011087mg [Citrus sinensis])

HSP 1 Score: 865.5 bits (2235), Expect = 4.4e-248
Identity = 423/490 (86.33%), Postives = 460/490 (93.88%), Query Frame = 1

Query: 7   PKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDT 66
           PK+RVAFVLIDGLGDVS+P+FG+KTPLQ A VPNLDAIASAG NGL+DPVEVGLGCGSDT
Sbjct: 7   PKRRVAFVLIDGLGDVSLPRFGYKTPLQAAYVPNLDAIASAGVNGLLDPVEVGLGCGSDT 66

Query: 67  AHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126
           AHLSLLGY+PRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE
Sbjct: 67  AHLSLLGYNPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126

Query: 127 EEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186
           EEGPILCAALDRMK+PSFP+YEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL Q
Sbjct: 127 EEGPILCAALDRMKLPSFPQYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLFQ 186

Query: 187 AEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVP 246
           A+ALDD++EAK TAAVVNELS+EIS+ILVSHP+NAKRAAEGKNIAN+VLLRGCGIRIEVP
Sbjct: 187 AQALDDTDEAKHTAAVVNELSREISKILVSHPLNAKRAAEGKNIANVVLLRGCGIRIEVP 246

Query: 247 PFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQ 306
            FEKKHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRT+LTSKATAIAK LSAPLQ
Sbjct: 247 SFEKKHGLWPCMVAPTKIIAGLGLSLDIDILEAPGATGDYRTILTSKATAIAKALSAPLQ 306

Query: 307 SSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKL 366
           S PNVFVPGEDE KPGR+DGYDFGFLH+KA+DDAGHDKA+IFKVK +EAVDRAIGQLA+L
Sbjct: 307 SCPNVFVPGEDEHKPGRSDGYDFGFLHIKAVDDAGHDKASIFKVKALEAVDRAIGQLARL 366

Query: 367 LWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLD 426
           LW+AE SG FQY+LCVTGDHSTPVEYGDHSFEPVP  MC+LKDFVGAVGGES +  I LD
Sbjct: 367 LWQAEKSGKFQYFLCVTGDHSTPVEYGDHSFEPVPLAMCQLKDFVGAVGGESAVMEIPLD 426

Query: 427 PFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMG 486
           PFPLPTV  GEDL   E  K+E+ER+++ QAF GDSVCEF+E++AA+GCLGRFPGGEMMG
Sbjct: 427 PFPLPTVKAGEDL--TEDKKIEKERSEQLQAFSGDSVCEFNEVAAARGCLGRFPGGEMMG 486

Query: 487 IIKKFLKLDA 497
           IIK +LKL A
Sbjct: 487 IIKTYLKLKA 494

BLAST of Cucsa.238290.1 vs. NCBI nr
Match: gi|567876529|ref|XP_006430854.1| (hypothetical protein CICLE_v10011558mg [Citrus clementina])

HSP 1 Score: 865.1 bits (2234), Expect = 5.8e-248
Identity = 424/490 (86.53%), Postives = 460/490 (93.88%), Query Frame = 1

Query: 7   PKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGLGCGSDT 66
           PK+RVAFVLIDGLGDVS+P+FG+KTPLQ A VPNLDAIASAG NGLMDPVEVGLGCGSDT
Sbjct: 7   PKRRVAFVLIDGLGDVSLPRFGYKTPLQAAYVPNLDAIASAGVNGLMDPVEVGLGCGSDT 66

Query: 67  AHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126
           AHLSLLGY+PRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE
Sbjct: 67  AHLSLLGYNPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFE 126

Query: 127 EEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186
           EEGPILCAALDRMK+PSFP+YEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ
Sbjct: 127 EEGPILCAALDRMKLPSFPQYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQ 186

Query: 187 AEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCGIRIEVP 246
           A+ALDD++EAK TAAVVNELS+EIS+ILVSHP+NAKRAAEGKNIAN+VLLRGCGIRIEVP
Sbjct: 187 AQALDDTDEAKHTAAVVNELSREISKILVSHPLNAKRAAEGKNIANVVLLRGCGIRIEVP 246

Query: 247 PFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKGLSAPLQ 306
            FEKKHGLWPCMVAPTKIIAGLGLSL IDIL+APGATGDYRT+LTSKATAIAK LSAPLQ
Sbjct: 247 SFEKKHGLWPCMVAPTKIIAGLGLSLDIDILEAPGATGDYRTILTSKATAIAKALSAPLQ 306

Query: 307 SSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKL 366
           S PNVFVPGEDE KPGR+DGYDFGFLH+KA+DDAGHDKA+IFKVK +EAVDRAIGQLA+L
Sbjct: 307 SCPNVFVPGEDEHKPGRSDGYDFGFLHIKAVDDAGHDKASIFKVKALEAVDRAIGQLARL 366

Query: 367 LWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVIDAISLD 426
           LW+AE SG FQY+LCVTGDHSTPVEYGDHSFEPVP  MC+LKDFVGAVGGES +  I  D
Sbjct: 367 LWQAEKSGKFQYFLCVTGDHSTPVEYGDHSFEPVPLAMCQLKDFVGAVGGESAVMEIPPD 426

Query: 427 PFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFPGGEMMG 486
           PFPLPTV  GEDL   E  K+E+ER+++ QAF GDSVCEF+E++AA+GCLGRFPGGEMMG
Sbjct: 427 PFPLPTVKAGEDL--TEDTKIEKERSEQLQAFSGDSVCEFNEVAAARGCLGRFPGGEMMG 486

Query: 487 IIKKFLKLDA 497
           IIK +LKL A
Sbjct: 487 IIKTYLKLKA 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APGM_METS51.5e-6738.292,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Metallosphaera se... [more]
APGM1_METTH2.0e-6737.852,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 OS=Methanothermoba... [more]
APGM_IGNH42.9e-6635.832,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Ignicoccus hospit... [more]
APGM_SULSO5.5e-6537.762,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfolobus solfat... [more]
APGM_SULTO7.2e-6536.542,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfolobus tokoda... [more]
Match NameE-valueIdentityDescription
A0A0A0KSM0_CUCSA3.0e-288100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643310 PE=4 SV=1[more]
A0A067F5X4_CITSI3.1e-24886.33Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011087mg PE=4 SV=1[more]
V4UWH8_9ROSI4.0e-24886.53Uncharacterized protein OS=Citrus clementina GN=CICLE_v10011558mg PE=4 SV=1[more]
A0A061F8H4_THECC1.3e-24686.21Cofactor-independent phosphoglycerate mutase OS=Theobroma cacao GN=TCM_031895 PE... [more]
M5WS34_PRUPE1.3e-24687.02Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004729mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30841.11.6e-23582.00 Cofactor-independent phosphoglycerate mutase[more]
AT4G09520.13.6e-23280.57 Cofactor-independent phosphoglycerate mutase[more]
Match NameE-valueIdentityDescription
gi|778707549|ref|XP_011656023.1|4.4e-288100.00PREDICTED: uncharacterized protein LOC101219404 [Cucumis sativus][more]
gi|659119038|ref|XP_008459441.1|1.3e-27696.77PREDICTED: uncharacterized protein LOC103498576 [Cucumis melo][more]
gi|1000961179|ref|XP_015576042.1|1.2e-24887.17PREDICTED: LOW QUALITY PROTEIN: probable 2,3-bisphosphoglycerate-independent pho... [more]
gi|641839969|gb|KDO58892.1|4.4e-24886.33hypothetical protein CISIN_1g011087mg [Citrus sinensis][more]
gi|567876529|ref|XP_006430854.1|5.8e-24886.53hypothetical protein CICLE_v10011558mg [Citrus clementina][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004456Pglycerate_mutase_ApgM
IPR006124Metalloenzyme
IPR017849Alkaline_Pase-like_a/b/a
IPR017850Alkaline_phosphatase_core_sf
Vocabulary: Molecular Function
TermDefinition
GO:00465372,3-bisphosphoglycerate-independent phosphoglycerate mutase activity
GO:0003824catalytic activity
GO:0046872metal ion binding
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0006007 glucose catabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046537 2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0046872 metal ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.238290Cucsa.238290gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.238290.1Cucsa.238290.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.238290.1.CDS.5Cucsa.238290.1.CDS.5CDS
Cucsa.238290.1.CDS.4Cucsa.238290.1.CDS.4CDS
Cucsa.238290.1.CDS.3Cucsa.238290.1.CDS.3CDS
Cucsa.238290.1.CDS.2Cucsa.238290.1.CDS.2CDS
Cucsa.238290.1.CDS.1Cucsa.238290.1.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.238290.1.three_prime_UTR.1Cucsa.238290.1.three_prime_UTR.1three_prime_UTR


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.238290.1.five_prime_UTR.3Cucsa.238290.1.five_prime_UTR.3five_prime_UTR
Cucsa.238290.1.five_prime_UTR.2Cucsa.238290.1.five_prime_UTR.2five_prime_UTR
Cucsa.238290.1.five_prime_UTR.1Cucsa.238290.1.five_prime_UTR.1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0044562,3-bisphosphoglycerate-independent phosphoglycerate mutasePFAMPF10143PhosphMutasecoord: 45..220
score: 1.2
IPR006124MetalloenzymePFAMPF01676Metalloenzymecoord: 9..405
score: 1.2
IPR017849Alkaline phosphatase-like, alpha/beta/alphaGENE3DG3DSA:3.40.720.10coord: 208..269
score: 5.1E-23coord: 8..88
score: 5.1E-23coord: 308..405
score: 5.1
IPR017850Alkaline-phosphatase-like, core domainunknownSSF53649Alkaline phosphatase-likecoord: 8..78
score: 3.92E-26coord: 204..404
score: 3.92
NoneNo IPR availablePANTHERPTHR31209FAMILY NOT NAMEDcoord: 324..415
score: 1.6E-270coord: 453..494
score: 1.6E-270coord: 3..303
score: 1.6E
NoneNo IPR availablePANTHERPTHR31209:SF0COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASEcoord: 453..494
score: 1.6E-270coord: 324..415
score: 1.6E-270coord: 3..303
score: 1.6E