Cucsa.232810.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.232810.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Calcium-binding EF-hand family protein, putative
Location	scaffold01989 : 615425 .. 616221 (+)
Sequence length	797

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAAACTCCACATCAAATCCCAAAACACAACCCACATAGTTTTCAACAATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGAAGAAGTTGTAAAATTTATGTACTTTTCTATGTTATCATCATATTATATATCTAGTACGTACTCATTAAGAAAAACTAGAATAAAGAGTTTGTTCTTGGAGGACGCATGATCAACTCTTACTATATATATATATATATTGCTCCTCTCTTTGGTTTATTGTTGTGTGTATTTGTCATGTTATCGTCTTCTAATATAAATCTTTCCATATTCATAAATATTTACATGACGAATTAATAAAGATGTTTGTCTTTGATCGAACTAA

mRNA sequence

TCCAAACTCCACATCAAATCCCAAAACACAACCCACATAGTTTTCAACAATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGAAGAAGTTGTAAAATTTATGTACTTTTCTATGTTATCATCATATTATATATCTAGTACGTACTCATTAAGAAAAACTAGAATAAAGAGTTTGTTCTTGGAGGACGCATGATCAACTCTTACTATATATATATATATATTGCTCCTCTCTTTGGTTTATTGTTGTGTGTATTTGTCATGTTATCGTCTTCTAATATAAATCTTTCCATATTCATAAATATTTACATGACGAATTAATAAAGATGTTTGTCTTTGATCGAACTAA

Coding sequence (CDS)

ATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGA

Protein sequence

MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK*

Homology

[Close]

BLAST of Cucsa.232810.1 vs. TrEMBL
Match: A0A0A0LNG6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 5.8e-87
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK
Sbjct: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN
Sbjct: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK
Sbjct: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 161

[Close]

BLAST of Cucsa.232810.1 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 9.7e-18
Identity = 59/161 (36.65%), Postives = 90/161 (55.90%), Query Frame = 1


Query: 19  SEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK--------------PQKV 78
           ++ EA+ LIKKYD+NGD +LTK+EL + + +   S     I+               QKV
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKV 69

Query: 79  E------PKVNIPDE-KASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCG 138
                  PK  + D+ + S     + LS+++++EIF  HD + DG+L+  EL KAF+  G
Sbjct: 70  SSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMG 129

Query: 139 SFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINK 159
           S   F KA YA+  AD + DG +   ELDK+++YA ++I K
Sbjct: 130 SIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKK 170

[Close]

BLAST of Cucsa.232810.1 vs. TrEMBL
Match: A0A0A0LP46_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358880 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.2e-17
Identity = 53/143 (37.06%), Postives = 90/143 (62.94%), Query Frame = 1


Query: 18  MSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKVEPKVNIPDEKASC 77
           +SE+EA+ L++  D NGD +L K EL A +     +H +++       P  ++ D     
Sbjct: 14  LSEKEANLLLEMRDANGDGVLDKQELMADL-----THTNKK-------PSTSLQD----- 73

Query: 78  GPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGD 137
               M+L+++E++EIF+  DIDGDG+L+++E+++AF M GS +   KA+YA+  AD +GD
Sbjct: 74  ----MKLTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGD 133

Query: 138 GFICLDELDKVLEYADRVINKTK 161
           G I   EL+K+++Y  R IN+ +
Sbjct: 134 GRISEPELNKLIDYVQRTINRRR 135

[Close]

BLAST of Cucsa.232810.1 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.0e-14
Identity = 42/77 (54.55%), Postives = 53/77 (68.83%), Query Frame = 1


Query: 78  GPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGD 137
           G  K   S++EM+EIF EHDIDGDGYL+ SEL+KA    G    F KA+Y +   D NGD
Sbjct: 5   GVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGD 64

Query: 138 GFICLDELDKVLEYADR 155
           G I  DELDK+++YA+R
Sbjct: 65  GLISEDELDKLIDYAER 81

[Close]

BLAST of Cucsa.232810.1 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.0e-14
Identity = 58/165 (35.15%), Postives = 83/165 (50.30%), Query Frame = 1


Query: 18  MSEQEASNLIKKYDKNGDSILTKD-------------ELTALIIEQAASHHHQQIKPQKV 77
           M+  EAS LIK+Y+KNGD++  K              E    I++ +    H+      +
Sbjct: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI 69

Query: 78  EPK-----------VNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAF 137
           +P              + D K+S     M LS++ ++EIF  HD + DG+L R EL KAF
Sbjct: 70  QPTQRTGGKGKKSITRVLDRKSS----NMSLSRERIKEIFQYHDNNNDGFLNRMELTKAF 129

Query: 138 NMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINK 159
              GS   F KA Y +  ADAN DG I   ELDK+++YA ++I K
Sbjct: 130 AFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKK 170

[Close]

BLAST of Cucsa.232810.1 vs. NCBI nr
Match: gi|449470192|ref|XP_004152802.1| (PREDICTED: calcium-binding protein-like [Cucumis sativus])

HSP 1 Score: 328.2 bits (840), Expect = 8.3e-87
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK
Sbjct: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN
Sbjct: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK
Sbjct: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 161

[Close]

BLAST of Cucsa.232810.1 vs. NCBI nr
Match: gi|659089537|ref|XP_008445563.1| (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo])

HSP 1 Score: 277.3 bits (708), Expect = 1.7e-71
Identity = 136/161 (84.47%), Postives = 149/161 (92.55%), Query Frame = 1


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTN L D  VPAQGNYMSEQEA+ LIKKYDKNGDSILTKDELTALI+EQAASHHHQQ+K
Sbjct: 1   MSTNALNDHHVPAQGNYMSEQEANTLIKKYDKNGDSILTKDELTALIMEQAASHHHQQVK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPK  +PDEKAS GPYKMRLS+DEMREIFLEHDIDGDG+LTRSEL+KAFNMCGS N
Sbjct: 61  PQKVEPK-KVPDEKASGGPYKMRLSRDEMREIFLEHDIDGDGHLTRSELIKAFNMCGSLN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADA+GD +I +DEL+KVL+YADRVIN+ KK
Sbjct: 121 SFSKANYALNLADADGDNYISMDELEKVLDYADRVINRPKK 160

[Close]

BLAST of Cucsa.232810.1 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 98.2 bits (243), Expect = 1.4e-17
Identity = 59/161 (36.65%), Postives = 90/161 (55.90%), Query Frame = 1


Query: 19  SEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK--------------PQKV 78
           ++ EA+ LIKKYD+NGD +LTK+EL + + +   S     I+               QKV
Sbjct: 10  AKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKV 69

Query: 79  E------PKVNIPDE-KASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCG 138
                  PK  + D+ + S     + LS+++++EIF  HD + DG+L+  EL KAF+  G
Sbjct: 70  SSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFSSMG 129

Query: 139 SFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINK 159
           S   F KA YA+  AD + DG +   ELDK+++YA ++I K
Sbjct: 130 SIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKK 170

[Close]

BLAST of Cucsa.232810.1 vs. NCBI nr
Match: gi|449470196|ref|XP_004152804.1| (PREDICTED: probable calcium-binding protein CML36 [Cucumis sativus])

HSP 1 Score: 97.1 bits (240), Expect = 3.1e-17
Identity = 53/143 (37.06%), Postives = 90/143 (62.94%), Query Frame = 1


Query: 18  MSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKVEPKVNIPDEKASC 77
           +SE+EA+ L++  D NGD +L K EL A +     +H +++       P  ++ D     
Sbjct: 14  LSEKEANLLLEMRDANGDGVLDKQELMADL-----THTNKK-------PSTSLQD----- 73

Query: 78  GPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGD 137
               M+L+++E++EIF+  DIDGDG+L+++E+++AF M GS +   KA+YA+  AD +GD
Sbjct: 74  ----MKLTREEIKEIFVGFDIDGDGFLSKNEVIQAFGMMGSCSPIMKAHYAMACADEDGD 133

Query: 138 GFICLDELDKVLEYADRVINKTK 161
           G I   EL+K+++Y  R IN+ +
Sbjct: 134 GRISEPELNKLIDYVQRTINRRR 135

[Close]

BLAST of Cucsa.232810.1 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 95.9 bits (237), Expect = 6.9e-17
Identity = 56/154 (36.36%), Postives = 90/154 (58.44%), Query Frame = 1


Query: 22  EASNLIKKYDKNGDSILTKDELTALI---------------IEQAASHHHQQIKPQ-KVE 81
           EAS LIKKYD+NGD +LTK+EL + +               I +   +  Q++    +++
Sbjct: 13  EASELIKKYDRNGDGVLTKEELQSFLHDARLKNIRFVSSSTIPKTNENLDQKVSSNTRIK 72

Query: 82  PKVNIPDE-KASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSK 141
           PK  + D+ + S     + LS+++++EIF  HD + DG+L+  EL KAF   GS   F+K
Sbjct: 73  PKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGSIVPFAK 132

Query: 142 ANYALNLADANGDGFICLDELDKVLEYADRVINK 159
           A +A+  AD + DG I   EL K+++YA+++I K
Sbjct: 133 ARHAMAYADVDEDGLISEAELHKLIDYAEKIIKK 166

The following BLAST results are available for this feature:

BLAST of Cucsa.232810.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNG6_CUCSA	5.8e-87	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359930 PE=4 SV=1	[more]
A0A0A0LRC8_CUCSA	9.7e-18	36.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1	[more]
A0A0A0LP46_CUCSA	2.2e-17	37.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358880 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	1.0e-14	54.55	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	1.0e-14	35.15	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]

BLAST of Cucsa.232810.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470192\|ref\|XP_004152802.1\|	8.3e-87	100.00	PREDICTED: calcium-binding protein-like [Cucumis sativus]	[more]
gi\|659089537\|ref\|XP_008445563.1\|	1.7e-71	84.47	PREDICTED: probable calcium-binding protein CML32 [Cucumis melo]	[more]
gi\|449470190\|ref\|XP_004152801.1\|	1.4e-17	36.65	PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus]	[more]
gi\|449470196\|ref\|XP_004152804.1\|	3.1e-17	37.06	PREDICTED: probable calcium-binding protein CML36 [Cucumis sativus]	[more]
gi\|659089539\|ref\|XP_008445564.1\|	6.9e-17	36.36	PREDICTED: probable calcium-binding protein CML11 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.232810	Cucsa.232810	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.232810.1	Cucsa.232810.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232810.1.five_prime_UTR.1	Cucsa.232810.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232810.1.CDS.1	Cucsa.232810.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232810.1.three_prime_UTR.1	Cucsa.232810.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 87..145 score: 5.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 22..50 score: 2.1coord: 88..116 score: 7.7E-4coord: 124..152 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 84..119 score: 13.816coord: 120..155 score: 9.52coord: 18..53 score: 10
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 100..150 score: 5.7E-12coord: 18..99 score: 6.5
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 15..52 score: 1.56E-18coord: 89..149 score: 1.56
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 133..145 score: -coord: 97..109 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 80..152 score: 1.6E-14coord: 15..51 score: 1.6