Cucsa.232190.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.232190.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	tRNA wybutosine-synthesizing protein 1-like protein
Location	scaffold01989 : 264384 .. 268975 (+)
Sequence length	2005

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCGATCAATTAACCTAACAGCATCTCAAAACCAATCAATTCGCCATGTCCCTCTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCATTAAACCCTACTCTTCCTTCTCGCAAACCGAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTCTGCTCAACGTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTACGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTCGCTAACTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAGTTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGTTGAAGGCATTGGGTGCGAAGGAGATTTTACCGATTGGTGAAGGAGACGTGGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTATGAAGTTTGATGCCGAGGGAAATGGGGTGGAGTTATGTTCTGGGATAGCTGGGGACAGTGATGCTGTAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGATATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAGTCTACGAATATAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGTAAGTTTGTTGGCTGTTTATATGCATATTTGTAATCATAAATATGGTTCCACGCATCTGCGTTTTCCAAATTGATCGAATAACTGCTTTTTTAAGGCCATTCCGAGTGTCTTGTGAATCAATACAAATTTGTACTGACTTGATAATTTGTTCTTGCAAGGGGCTAGTTGGGTTGAATTAAAAGCCTTACCTCTTTTTCTTGTGTTGTTTGGTTGGCTCTTTGAACTATTTTTCTTATGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTAAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGGTAATGTCCCACTGTATATGCTATTCTGCTTGGCCATAGCGTGTCTTATTTCCTTTTCATGGTCATTATAAATTTGGAATTCTTGATGCTGTAATGCATTAAGTGGCAATGTCATGGAGATCCCCCTTCATTAAAATGTATGCCACTTTACTTTGGTTAATATATAATTTCAATTCTTGAGAAGAATGGATGATAGAATATGATTGAATACTGACTTGGTGAATAATGAGAATAGATTGGCCGTGGCCTTCAAAGCACTGCCGAATTCACAATATTAAATGGAGATCACTTTGTTTGATATTCTTTGGTCGGCACGGCCAAATATTATATCTAGCAGCTACTTCTAATTTCATTCAGGCAATCTTTAACAGTCTGCCTGCGTGATTAATATCTTAAATATCCTCTTTTAGTTGTTAACGCTGGTGCAAGATGTGTGTTCAGTGGTCCTTTTGTTCGACTTGCATTATGAGTTCTTATTCATGCCTGTTAAGTTCACTTCTTTGGGCTCTGGGAAATTTCAAGTTGACTCTTCACTTTCCTTTCACTCAACAAGGCTTTTCATAAGCTCTTGACTTTTTTAATTGACTGGTTAAAACTATCTATTGATCAGTGTTATTGCTCACAGTAACTTTTTTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAATAAATGTGTTTtCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGCTGGCAGTGGGAGATGGATGATCCATTGGAAATTGTGAATTCTGCAATAGATTTACACACAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTTAGTGCTCCTGCTTTACCGAATTGGCTTTAGTAATATTTTTATGAGGAATGTCTTTTCTTGGATATACTCTATTGTTTCTGTGATTTTCCGGTTGTGGGATCTGAAATGTTGGTAAATCATCAATATTTTCACTTTTAATTGTTCTTTTAGAACTCTCATAAAAATGTGTTGGCGTGGTATGGGTTTTGTGGCTAAAAAGGAATCGGGAGCAGTAGAGACAGAAAGTAACCAGCATATTATTCGGTTGAATTTTCCTTTGAGAGTTTTAGAGATCTTTTGGAAATTTAAGCAATATGTAATCTAAGATTCCTGAGTCTCTTGCTGCTTGAGAATGTAAAGTTTTGGTATTTTATTTTATAGTAGCCATCACTTGGGGTCTTGTGCGGGTATAGAAATGCATTGGCATAGCATGAGTTTTGTAGCCACACAGAGATCAAGAGTAGTAGGGACCAAAAGTAACCTGTATTTTATGCAGTTGAATTTTCCTTTGGGAGTTTTAGAGATCTTTTGAAGGAATTTAAGCAATATGTTTAATTTAAGATCCTTTTATCTGGCTGCTTTGAGAATGCAAAGTTGAGGTATTTTATTTTGCAGTAGCCAATAGCCAACAGTGAGAGAATTGTGAGGATGCTTGATATGCAAGTTATTTTTCATATTCTTTTTACTGGTAAATTTCAAACTTTTTTTtGGGCAGTAATCTATGAAATATAATCGATGAGTTTATGTTCATATAAACCTTTTTATTTTtAGTTATTTATTTATTTTATTCAGAAAGAACAAAGAAGTACATGTCATGAAATTTAGAAGGCCATGCTTGCTTATGCAAGCTTGCTCCTTGCTAAAACAAGTGTAAACGTAATTTTAGTTATTCAGATCCACTTATGTTAATAAAAGAGAAGTTAATGAACCATGAATGCTTTAAGGTGTAAAAGCATATGGTGGTAGTTTGAGATTTTAATGACAAGGTGAGTCATTAATGCATGTCGTTTTGTTGATAAGCAATGTCTTCCATCTTCCTGCAGGTGTTACGCAGGAGAAATTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAAATTGCTGAAGCCGGTCACCCAGGTTTGTGTGCTTTCGAATTTTtGTTTtCTAAATAACATCAAATATCATGATTATATTCACGAATCATGTTACTAGATTACCTATCTAATTTTTATGATGCATGCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGTAAGTTTCCTCACCCCTTCACCCCTTTCCTAGTTTGTCTCTTGAGCCAGGCTTCATATGAACTAATGAGAGAAGTAGATCTTGGGGGGGTGTTTATATCTTGTTGCTTTGTTGCCTTGTTGCCGTAATCTGCTTTGTAGTTTACTGTCCTTCTTTTTCATGTAGAGATGCAAAAGATTTGCAGTTTCTTTACAAAATCCTAAAAGACAATCTAGTTTCTTGATTACATTGTCAATTGGGCTGGATAAGGATCAGATGTTTCTTGTCATTTTGTCATCACACACAATAACTTAAGTTCTTTGACTATTGCAATTATTCTTGGCCTTAAATTCTGTGGACAGAAGGAAATATTCTTTAAATAACGGATTTGTGTGCTCCTATAATGTTGCAGGATTCTTTGAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGATGCATATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAGGGCGTCACATATTGTGGATCGTGAGTTCATATTTATTTATTTTGCACTAGATGGCAGGATATTGATTTGAAAATTGTGGGAGCTTAACGGATCTAATGTTCTTCTGTAGATCTACTACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAaGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGCGTTCTTTTAGCTAAAACTGACAAGTTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGATAAATTCCATGACCTGGTTTGTATAGACAACTAATTTACAATAAAaCTTTAAATTGTTGAAGCTCCTTCCATTAAATTTATCCTTTTTGAAAATTTCAGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGATACAGGAAGGAGCGACATCATAAACCAAAACCCACATCCTGACAGCTTTGAATGTCCGGGTCAGTTTTGTTTAATGGG

mRNA sequence

GTTCGATCAATTAACCTAACAGCATCTCAAAACCAATCAATTCGCCATGTCCCTCTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCATTAAACCCTACTCTTCCTTCTCGCAAACCGAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTCTGCTCAACGTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTACGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTCGCTAACTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAGTTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGTTGAAGGCATTGGGTGCGAAGGAGATTTTACCGATTGGTGAAGGAGACGTGGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTATGAAGTTTGATGCCGAGGGAAATGGGGTGGAGTTATGTTCTGGGATAGCTGGGGACAGTGATGCTGTAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGATATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAGTCTACGAATATAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTAAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAATAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGCTGGCAGTGGGAGATGGATGATCCATTGGAAATTGTGAATTCTGCAATAGATTTACACACAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTGTTACGCAGGAGAAATTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAAATTGCTGAAGCCGGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGATTCTTTGAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGATGCATATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAGGGCGTCACATATTGTGGATCATCTACTACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGCGTTCTTTTAGCTAAAACTGACAAGTTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGATAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGATACAGGAAGGAGCGACATCATAAACCAAAACCCACATCCTGACAGCTTTGAATGTCCGGGTCAGTTTTGTTTAATGGG

Coding sequence (CDS)

ATGTCCCTCTCTTCACGCTCCGCAAAGCTCACCATTCTAGCTCTTCTCTCCGCCTCTACTTTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCATTAAACCCTACTCTTCCTTCTCGCAAACCGAAACTGTTTTTCATTTCTCAAACTGGTACCTCCAAGGCCCTAGCTCATCGTCTGCTCAACGTTTTGAATTCCAATGGGTTGGTTTTCGATCTTGTTGATCCCAAGGATTACGAGCCTGAGGATCTTTTTAAGGAAACTTTAGTTTTGTTTGTTGCTTCCACTTGGGAAGACGGAGGACCGCCTCCTCATGCTAAATTTCTCGCTAACTGGCTATCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTCAGTGTAAGTTTTCTGTGTTTGGGGTTGGTAGTCGAGTTTATGGAGAGACATTCAATGCGGTTGCAAGGGATTTTTCCAAGCGGTTGAAGGCATTGGGTGCGAAGGAGATTTTACCGATTGGTGAAGGAGACGTGGATGGAGGTGATATAGATAAGTGTTTTGATGATTGGAGTAGGAAATTGCTGAAGGTTATGAAGTTTGATGCCGAGGGAAATGGGGTGGAGTTATGTTCTGGGATAGCTGGGGACAGTGATGCTGTAAGTGTTGAGGAGGATGAAGAAGAAGATGACGACCTTGGGGAGGAGGATATCGTTGATCTTGAGGATATTGCCGGTAAGGGCCCTTCGAGAAAGTCTACGAATATAGTTGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACCCCGGTGATTAGAGCGAGCTTGGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTAAAAATATGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAATAAATGTGTTTtCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGCTGGCAGTGGGAGATGGATGATCCATTGGAAATTGTGAATTCTGCAATAGATTTACACACAAAGATGATCAAGCAAATGAAAGGTGTTCCGGGTGTTACGCAGGAGAAATTGGAGGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGGCGGATTTCAACTTTCTTGGTGACAAATGCTCAGTTTCCTGATAAAATAAAATTGCTGAAGCCGGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGGGATTTCTGGGAGCGATTCATTGATTCTTTGAAAGCTCTAAATGAAAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTGAAGGGGTGGAACACAGAAGATATCGATGCATATTCGAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAGGGCGTCACATATTGTGGATCATCTACTACATCAAAGCTGACAATGGAGAATGTTCCTTGGCATTCTGATGTGAAGTCTTTTTCGGAGGCTTTAGCTGCAAAAaGTCAAGGGGAGTATGAAGTTGCTTGTGAGCATGTGCACTCGTGTTGCGTTCTTTTAGCTAAAACTGACAAGTTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTACGATAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCTGACGAAGGTGGATTTGATCCTGATCAATCTCGATACAGGAAGGAGCGACATCATAAACCAAAACCCACATCCTGA

Protein sequence

MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS*

Homology

[Close]

BLAST of Cucsa.232190.1 vs. Swiss-Prot
Match: TYW1_ARATH (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana GN=TYW1 PE=2 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 2.5e-280
Identity = 468/640 (73.12%), Postives = 536/640 (83.75%), Query Frame = 1


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLPSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S R+  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVMKFDA--EGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  +  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDY+KFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

[Close]

BLAST of Cucsa.232190.1 vs. Swiss-Prot
Match: TYW1_ORYSJ (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica GN=TYW1 PE=2 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 1.0e-257
Identity = 438/641 (68.33%), Postives = 511/641 (79.72%), Query Frame = 1


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL--PSRKPKLFFISQTGTSKALA 63
           +S ++ L +L LLS+S+++  YKS RLR+      P    P+  P L + S TGTSKALA
Sbjct: 12  ASTTSHLALLLLLSSSSVFFLYKSLRLRRNNPPSPPPGQGPAPTPTLLYASATGTSKALA 71

Query: 64  HRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
             L   L   G+     D   ++P+DL    L+L V  T + G PPP A FLA WL ESA
Sbjct: 72  AGLSRRLAEAGVTAHPADAAAFDPDDLPSLPLLLLVLPTHDGGAPPPAAAFLARWLEESA 131

Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGGD 183
            DFR G+ LLS  +F+VFGVGSR YGETFNA AR FS+ L+ALGA E++ +GEGDVDGGD
Sbjct: 132 ADFRAGAALLSGLRFAVFGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGD 191

Query: 184 IDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDI----V 243
           ++  F++W  ++++V+K      G E+  G  G+SD     E+EE DDD  EE++    V
Sbjct: 192 LEVVFEEWCGRVVRVVK------GEEIGEGHNGESDGFDELEEEESDDDDDEEEVDGGEV 251

Query: 244 DLEDIAGKGPS-RKSTNIVE---TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 303
           D+EDIAGK P+ R+    VE   +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKIC
Sbjct: 252 DMEDIAGKAPAARRRNGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKIC 311

Query: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 363
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDD
Sbjct: 312 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDD 371

Query: 364 PLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 423
           PL+IVN+AID HTKM+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DEL
Sbjct: 372 PLDIVNAAIDQHTKMVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDEL 431

Query: 424 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 483
           HRR ISTFLVTNAQFPDKIK LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSLK
Sbjct: 432 HRRHISTFLVTNAQFPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLK 491

Query: 484 ALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENV 543
           +L++K QRTVYRLTLVKGWN E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 492 SLHDKDQRTVYRLTLVKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENV 551

Query: 544 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLV 603
           PWHSDVK FSEALA KS G YEVACEH HSCCVLLAK DKF+++G+W TWIDYD+FH+LV
Sbjct: 552 PWHSDVKDFSEALALKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELV 611

Query: 604 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHH 635
            SGKPF S+DYMA TPSWAVYG++EGGFDPDQSRY+KER H
Sbjct: 612 TSGKPFRSQDYMALTPSWAVYGAEEGGFDPDQSRYKKERRH 646

[Close]

BLAST of Cucsa.232190.1 vs. Swiss-Prot
Match: TYW1_XENLA (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Xenopus laevis GN=tyw1 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 4.0e-177
Identity = 326/652 (50.00%), Postives = 429/652 (65.80%), Query Frame = 1


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +   GL  +L++ K+Y+P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRL 166
            DG PP  A++   WL E++ DFR G   L   +++VFG+G+ VY   +N V ++  K L
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSG----- 226
             L A  ++   EGD +      G I+  F+ W RK L  +K    G   + CSG     
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGE-KKPCSGKCKKG 263

Query: 227 ---IAGDSDAVSVEEDEEEDDDLGEED------------------------IVDLEDIAG 286
                  S   SVEE+EEE++   E +                        ++D+ED+ G
Sbjct: 264 KCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDL-G 323

Query: 287 KGPS--RKSTNIVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSG 346
           K  S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSG
Sbjct: 324 KAMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSG 383

Query: 347 VKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW 406
           VK+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W
Sbjct: 384 VKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRW 443

Query: 407 EMDDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTL 466
           +MD P  I+  AI  H  MIKQ KGVPGV  E+ EEGL+ +HCALSLVGEPIMYPEINT 
Sbjct: 444 KMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTF 503

Query: 467 VDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI 526
           +  LH + IS+FLVTNAQFP++I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFI
Sbjct: 504 LRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFI 563

Query: 527 DSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLT 586
           DSLKAL EKQQRTVYRLTLVK WN +++ AY++L ++G PDF+E+KGVTYCG S+ S LT
Sbjct: 564 DSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLT 623

Query: 587 MENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKF 636
           M NVPWH +V  F E L      +YEVACEH HS C+L+A   KF+++G+W TWIDY++F
Sbjct: 624 MANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLIAH-KKFKINGEWCTWIDYERF 683

[Close]

BLAST of Cucsa.232190.1 vs. Swiss-Prot
Match: TYW1_DANRE (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Danio rerio GN=tyw1 PE=2 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 2.3e-172
Identity = 314/649 (48.38%), Postives = 424/649 (65.33%), Query Frame = 1


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKET-----LVLFVASTW 106
           K+F+ SQTGT+K  A  L   + + G+  +++D KD++PED   E      + +F+ +T+
Sbjct: 80  KVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRL 166
            DG P   A++   WL E++ DFR G   L   +++VFG+G+ VY   FN V++   K L
Sbjct: 140 TDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWL 199

Query: 167 KALGAKEILPIGEGDVD-----GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSG----- 226
             L A  I+  GEGD +      G +   F  W  K L  ++  A+G   + CSG     
Sbjct: 200 WMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGE-KKACSGNCKKA 259

Query: 227 ----------IAGDSDAVSVEEDEEE-------DDDLGEED------IVDLEDIAG---- 286
                      A D+ +++ + + EE       D++   ED      ++D+ED+      
Sbjct: 260 SCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGSVIDMEDLGNVMNH 319

Query: 287 --KGPSRKSTNIVETNG-KLNGKKVMVT---------PVIRASLEKQGYKIIGSHSGVKI 346
             K   R   +  ++   K NG++             P +R SL KQGYK+IGSHSGVK+
Sbjct: 320 MKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKL 379

Query: 347 CRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMD 406
            RWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+MD
Sbjct: 380 RRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMD 439

Query: 407 DPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDE 466
              +I+  A++ H  MI+Q +GVPGV  E+ EEGL+ +HCALSLVGEPIMYPEIN+ +  
Sbjct: 440 PAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKL 499

Query: 467 LHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSL 526
           LH++ IS+FLVTNAQFP++I+ L PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DSL
Sbjct: 500 LHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSL 559

Query: 527 KALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMEN 586
           +AL EKQQRTVYRLTLVK WN +++ AY++L ++G+PDF+E+KGVTYCG S+ S LTM N
Sbjct: 560 RALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMAN 619

Query: 587 VPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDL 636
           VPWH +V  F + L A    +YE+ACEH HS C+LLA   KF+VDG+W+TWIDY++F +L
Sbjct: 620 VPWHEEVIYFVQQL-ANLLPDYEIACEHEHSNCLLLA-NHKFKVDGEWWTWIDYERFQEL 679

[Close]

BLAST of Cucsa.232190.1 vs. Swiss-Prot
Match: TYW1_MOUSE (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Mus musculus GN=Tyw1 PE=1 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 3.4e-160
Identity = 306/654 (46.79%), Postives = 406/654 (62.08%), Query Frame = 1


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S  L   +++ K+Y+P+D     +  +T+  F+ +T+
Sbjct: 72  KIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATY 131

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSQCKFSVFGV-------------------- 166
            DG P   A++   WL ESA DFR G   L   +++VFG+                    
Sbjct: 132 TDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWL 191

Query: 167 ----GSRVY--GE--------TFNAVARDFSK-RLKALGAKEILPIGEGDVDGGDIDKCF 226
                 RV   GE           ++  DF+  + K +   + L  GE    GG+  +  
Sbjct: 192 WMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGK 251

Query: 227 DDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEED------IVDLE 286
            + ++      +   +G   EL  G A + +      +   +D+LG +D      +VD+E
Sbjct: 252 CESAQHGPGEARPHPQG---ELHPGDAEEEEPC----ESSSEDELGTQDYQSLTSVVDVE 311

Query: 287 DIAG--KGPSRKSTNIVETNGKL----NGKKV-------MVTPVIRASLEKQGYKIIGSH 346
           D+        R+       +GK     N +K        M+TP +R +L KQGY++IGSH
Sbjct: 312 DLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSH 371

Query: 347 SGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW 406
           SGVK+CRWTKS LRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHHTNPVG  W
Sbjct: 372 SGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEW 431

Query: 407 QWEMDDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEIN 466
           +W+MD P  I+  AI+ H  MIKQ KGVPG+  E+ EEG+  +HCALSLVGEPIMYPEIN
Sbjct: 432 RWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEIN 491

Query: 467 TLVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWER 526
            L+  LH+  IS+FLVTNAQFP++I+ L PVTQLYVSVDA+T++ LK IDRPLF DFW+R
Sbjct: 492 RLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQR 551

Query: 527 FIDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSK 586
           F+DSLKAL+ KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S  S 
Sbjct: 552 FLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASS 611

Query: 587 LTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYD 636
           LTM NVPWH +V  F   L      +YEVACEH HS C+L+    KF++DG+W+TWI+Y 
Sbjct: 612 LTMANVPWHEEVVRFVREL-VDLLPDYEVACEHEHSNCLLIGH-KKFKIDGEWWTWINYS 671

[Close]

BLAST of Cucsa.232190.1 vs. TrEMBL
Match: A0A0A0LPA4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G361620 PE=4 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of Cucsa.232190.1 vs. TrEMBL
Match: A0A067L1Y3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04425 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 2.8e-302
Identity = 507/647 (78.36%), Postives = 567/647 (87.64%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL--------PSRKPKLFFIS 60
           MSLSSRSA+LT+LA+LSAST YCFYKSRRL+ LK SLNP+L         S K KLFFIS
Sbjct: 1   MSLSSRSARLTLLAVLSASTFYCFYKSRRLKLLKDSLNPSLNRNSNSVCSSNKGKLFFIS 60

Query: 61  QTGTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKF 120
           QTGTSK LA RL N+   N L FDL+D KDYEPEDL KETLV+ VASTWEDG PP +A F
Sbjct: 61  QTGTSKNLAQRLHNLFKLNDLSFDLIDAKDYEPEDLSKETLVIIVASTWEDGKPPSNANF 120

Query: 121 LANWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPI 180
            +NWL+ESA+DFRVGSLLL+ CKF+VFGVGSR YG+TFNAVA+DFS+RL+ LGA EIL +
Sbjct: 121 FSNWLAESADDFRVGSLLLTNCKFAVFGVGSRAYGDTFNAVAKDFSRRLRELGANEILTV 180

Query: 181 GEGDVDGGDIDKCFDDWSRKLLKVMKFDAEGNG---VELCSGIAGDSDAVSVEEDEEEDD 240
           GEGDVDGG++D  F++WS K++ ++K     NG   +  C G   D++ +  E   ++D+
Sbjct: 181 GEGDVDGGELDGFFEEWSEKVVGILKGGRVENGEVFINGCIGSESDNENIVDEFGSDDDE 240

Query: 241 DLG-EEDIVDLEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHS 300
           ++G E  IVDLEDIAGKGP+RK   +VETNGKLNG+K MVTPVIRA+LEKQGYKIIGSHS
Sbjct: 241 NVGVESGIVDLEDIAGKGPTRKFNAVVETNGKLNGQKEMVTPVIRANLEKQGYKIIGSHS 300

Query: 301 GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQ 360
           GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK+WQ
Sbjct: 301 GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKNWQ 360

Query: 361 WEMDDPLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINT 420
           W+MDDPLEIVNSAID HTKMIKQMKGVPGVT E+L EGL PRHCALSLVGEPIMYPEINT
Sbjct: 361 WKMDDPLEIVNSAIDQHTKMIKQMKGVPGVTLERLTEGLHPRHCALSLVGEPIMYPEINT 420

Query: 421 LVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERF 480
           LVDELHRRRISTFLVTNAQFP+KI +LKPVTQLYVSVDAATKESLK+IDRPLFGDFWERF
Sbjct: 421 LVDELHRRRISTFLVTNAQFPEKITMLKPVTQLYVSVDAATKESLKSIDRPLFGDFWERF 480

Query: 481 IDSLKALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKL 540
           IDSLKAL EKQQRTVYRLTLVKGWNTEDID Y  LFSIG+PDF+EIKGVTYCGSS TSKL
Sbjct: 481 IDSLKALKEKQQRTVYRLTLVKGWNTEDIDDYFKLFSIGQPDFIEIKGVTYCGSSATSKL 540

Query: 541 TMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDK 600
           TMENVPWH+DVK+FSEALA KS+GEYEVACEH HSCCVLLA+T+KF+++GQWFTWIDY+K
Sbjct: 541 TMENVPWHADVKAFSEALALKSKGEYEVACEHAHSCCVLLARTEKFKINGQWFTWIDYEK 600

Query: 601 FHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 636
           FHDLVASGKPF+SKDY+A TP WAVYG+ EGGFDPDQSRYRKERHHK
Sbjct: 601 FHDLVASGKPFNSKDYVAATPFWAVYGAQEGGFDPDQSRYRKERHHK 647

[Close]

BLAST of Cucsa.232190.1 vs. TrEMBL
Match: A0A061FDJ1_THECC (Flavodoxin family protein / radical SAM domain-containing protein isoform 1 OS=Theobroma cacao GN=TCM_034037 PE=4 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 4.5e-300
Identity = 508/641 (79.25%), Postives = 569/641 (88.77%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRR----LRKLKLSLNPTLPSRKPKLFFISQTGT 60
           MS SS SA+LT+LALLSA+T Y  YKSRR    LR+L ++ NP    RK KLFFI+QTGT
Sbjct: 1   MSPSSLSARLTLLALLSATTFYFLYKSRRRLKPLRQLPINSNP----RKGKLFFITQTGT 60

Query: 61  SKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANW 120
           SKALA RLLN+L+SN + FDLVDP++YEPEDL KE+L+L +ASTWEDG PP  +KF  NW
Sbjct: 61  SKALAQRLLNLLSSNNIPFDLVDPQNYEPEDLPKESLILIIASTWEDGNPPQGSKFFVNW 120

Query: 121 LSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGD 180
           L+E + DFR G+LLLS CKF+VFGVGSRVYGETFNAVAR+  KRL+ LGA E++ +GEGD
Sbjct: 121 LAEISTDFRAGNLLLSSCKFAVFGVGSRVYGETFNAVARNLGKRLRGLGATEMVAVGEGD 180

Query: 181 VDGGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLG-EED 240
           VDGG ++  F +WS K+++V+K    G  VE  +GI   SDA S E D++++   G EE+
Sbjct: 181 VDGGALESVFKEWSEKVVRVLK---GGLVVENENGIVYASDAESFESDDDDNGGGGGEEE 240

Query: 241 IVDLEDIAGKGPSRK-STNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 300
           IVDLEDIAGKGPSRK S N+ ETNGK++GK+ MVTPVIRA+LEKQGYKIIGSHSGVKICR
Sbjct: 241 IVDLEDIAGKGPSRKKSVNVAETNGKMDGKREMVTPVIRANLEKQGYKIIGSHSGVKICR 300

Query: 301 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 360
           WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW+MDDP
Sbjct: 301 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDDP 360

Query: 361 LEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH 420
           LEIVN+AIDLHTKMIKQMKGVPGVTQE+L EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 361 LEIVNTAIDLHTKMIKQMKGVPGVTQERLTEGLTPRHCALSLVGEPIMYPEINMLVDELH 420

Query: 421 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 480
           RRRISTFLVTNAQFPDKIK+LKP+TQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKA
Sbjct: 421 RRRISTFLVTNAQFPDKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKA 480

Query: 481 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVP 540
           L +KQQRTVYRLTLVKGWNTED DAYS LFSIG+PDFVEIKGVTYCGSS TSKLTMENVP
Sbjct: 481 LRDKQQRTVYRLTLVKGWNTEDADAYSKLFSIGKPDFVEIKGVTYCGSSATSKLTMENVP 540

Query: 541 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVA 600
           WHSDVK+FSEALA KS+GEYEVACEH HSCCVLLAKTDKF+V+GQW+TWIDY+KFHDLVA
Sbjct: 541 WHSDVKAFSEALALKSEGEYEVACEHAHSCCVLLAKTDKFKVNGQWYTWIDYEKFHDLVA 600

Query: 601 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 636
           SG+PFDS DYMA TPSWAVYG++EGGFDPDQSRYRKERHHK
Sbjct: 601 SGEPFDSADYMALTPSWAVYGAEEGGFDPDQSRYRKERHHK 634

[Close]

BLAST of Cucsa.232190.1 vs. TrEMBL
Match: F6H045_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g12460 PE=4 SV=1)

HSP 1 Score: 1036.2 bits (2678), Expect = 1.7e-299
Identity = 506/646 (78.33%), Postives = 565/646 (87.46%), Query Frame = 1


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLP--SRKPKLFFISQTGTSKALA 63
           +S SA+LT+LALLSASTLYCFYKS RL++  L    T P  S K KLFF+SQTGTS+ LA
Sbjct: 8   NSHSARLTLLALLSASTLYCFYKSLRLKRHALLFPTTNPNSSSKGKLFFVSQTGTSEILA 67

Query: 64  HRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESA 123
            RLL +L S  L FDLV PKDYEPEDL KETLVL VASTWEDG PPP A F +NWL+ESA
Sbjct: 68  RRLLRLLTSCDLSFDLVHPKDYEPEDLSKETLVLIVASTWEDGNPPPDAGFFSNWLAESA 127

Query: 124 EDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGGD 183
           +DFRVGS+LLS+CKF+VFGVGSR YG  FNAVAR FSKR++ LG  ++LP+ EGDVD GD
Sbjct: 128 DDFRVGSMLLSRCKFAVFGVGSRSYGAAFNAVARGFSKRMRKLGGLQVLPVEEGDVDAGD 187

Query: 184 IDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSV---EEDEEEDDDLGEED--- 243
           +D+ FD WSRKL+ V+K  +  NGV   S    +SDA ++   EED ++DDD   E+   
Sbjct: 188 LDEVFDVWSRKLISVLKGGSVENGVFDGSMAVVESDAETIDGSEEDYDDDDDADRENGAE 247

Query: 244 --IVDLEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 303
             IVDLEDIAGKGPSRK+  +  ++G +NG+K MVTPVIRASLEKQGYKIIGSHSGVKIC
Sbjct: 248 GSIVDLEDIAGKGPSRKTKTMTTSSGTINGEKEMVTPVIRASLEKQGYKIIGSHSGVKIC 307

Query: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 363
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDD
Sbjct: 308 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDD 367

Query: 364 PLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 423
           PLEIVNSAIDLHTKMI+QMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN+L++EL
Sbjct: 368 PLEIVNSAIDLHTKMIRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIMYPEINSLIEEL 427

Query: 424 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 483
           HRRRISTFLVTNAQFP+KIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERF+DSLK
Sbjct: 428 HRRRISTFLVTNAQFPEKIKELKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLK 487

Query: 484 ALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENV 543
           AL EKQQRTVYRLTLVKGWNTE+IDAY NLFSIG+PDFVEIKGVTYCGSS TSKLTMENV
Sbjct: 488 ALREKQQRTVYRLTLVKGWNTEEIDAYFNLFSIGKPDFVEIKGVTYCGSSATSKLTMENV 547

Query: 544 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLV 603
           PWH+DVK+FSEALA KS+GEYEVACEHVHSCCVLLAKTDKF+V+ QWFTWIDY+KFHDLV
Sbjct: 548 PWHADVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTDKFKVNNQWFTWIDYEKFHDLV 607

Query: 604 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPT 640
           ASG+PF+SKDYMA TPSWAVYG++EGGFDP QSRYRKERHH+PK T
Sbjct: 608 ASGRPFNSKDYMAATPSWAVYGAEEGGFDPGQSRYRKERHHRPKTT 653

[Close]

BLAST of Cucsa.232190.1 vs. TrEMBL
Match: W9RQ06_9ROSA (tRNA wybutosine-synthesizing protein 1-like protein OS=Morus notabilis GN=L484_003329 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 7.9e-297
Identity = 508/692 (73.41%), Postives = 564/692 (81.50%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPS------RKPKLFFISQT 60
           M +SS  A+LT+LALLSA+T YCFYK  RLR L++SLNP   S      R+ KL FIS+T
Sbjct: 11  MCISSVPARLTLLALLSATTFYCFYKFHRLRSLRISLNPNPNSKSSSCFRRGKLLFISET 70

Query: 61  GTSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLA 120
           GTSK LA RLL++LNSN L FDLVD K YEPEDL KET VL VASTWEDG  PP  +F A
Sbjct: 71  GTSKTLARRLLDLLNSNDLAFDLVDAKAYEPEDLPKETQVLIVASTWEDGKAPPSGRFFA 130

Query: 121 NWLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGE 180
           NWL+ES EDFRVGSLLLSQCKF+VFGVGSR YG T+N VARD SKR++ LGA E+LP+ E
Sbjct: 131 NWLAESVEDFRVGSLLLSQCKFAVFGVGSRAYGATYNVVARDLSKRMRGLGAAEMLPVWE 190

Query: 181 GDVDGGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLG-E 240
           GDVDGGD+++ FD WS K++KV+  D   NG  L +G+  +SDA   E D E D++   E
Sbjct: 191 GDVDGGDLEEAFDAWSAKMVKVLNDDVVENGGALSNGVGAESDA---ESDYETDEEYSVE 250

Query: 241 EDIVDLEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 300
            +IVDLEDIAGKGPSR+S  +V+TNG  NGKK MVTPVIRASLEKQGYKIIGSHSGVK+C
Sbjct: 251 SEIVDLEDIAGKGPSRRSPVVVQTNGTSNGKKDMVTPVIRASLEKQGYKIIGSHSGVKVC 310

Query: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 360
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW+MDD
Sbjct: 311 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDD 370

Query: 361 PLEIVNSAIDLHTKMIKQMKGVP------------------------------------- 420
           PLEIVNSAID HTKMIKQMKGVP                                     
Sbjct: 371 PLEIVNSAIDQHTKMIKQMKGVPGSHFGRGDLERPEWAIQSGLLWTSVSEAWWYLGSWLS 430

Query: 421 --------------GVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFL 480
                         GVT E+L EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFL
Sbjct: 431 PTLLKYGLSISSCAGVTLERLTEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFL 490

Query: 481 VTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQRT 540
           VTNAQFP+KIK+LKP+TQLYVSVDAATK+SLKAIDRPLFGDFWERF+DSLKAL +KQQRT
Sbjct: 491 VTNAQFPEKIKMLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFLDSLKALRDKQQRT 550

Query: 541 VYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVKSF 600
           VYRLTLVKGWNTED+DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWHS+VK+F
Sbjct: 551 VYRLTLVKGWNTEDVDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHSEVKAF 610

Query: 601 SEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFDSK 635
           SEALA KSQGEYEVACEHVHSCCVLLAKT+KF+++GQWFTWI+Y+KFHDLVASGK FDSK
Sbjct: 611 SEALALKSQGEYEVACEHVHSCCVLLAKTEKFKINGQWFTWIEYEKFHDLVASGKAFDSK 670

[Close]

BLAST of Cucsa.232190.1 vs. TAIR10
Match: AT1G75200.1 (AT1G75200.1 flavodoxin family protein / radical SAM domain-containing protein)

HSP 1 Score: 965.7 bits (2495), Expect = 1.4e-281
Identity = 468/640 (73.12%), Postives = 536/640 (83.75%), Query Frame = 1


Query: 4   SSRSAKLTILALLSASTLYCFYKSRRLRKLK-LSLNP--TLPSRKPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + + K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN + FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 183
           AEDFRVGSLLLS CKF+VFGVGSR YGE++NAVA++ S R+  LG  E++P+GEGDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDKCFDDWSRKLLKVMKFDA--EGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVD 243
           ++D+ F DW   +++V+K  +  E NGV    G   D        DEE++D+  +  IVD
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVEDDLEYYDSTDEEDEDNDADGGIVD 244

Query: 244 LEDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKS 303
           LEDIAGK PS+++  +  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICRWTKS
Sbjct: 245 LEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICRWTKS 304

Query: 304 QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIV 363
           QLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P  IV
Sbjct: 305 QLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEPSVIV 364

Query: 364 NSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRI 423
             A+DLH  MIKQMKGVPGVT EKL+EGL+PRHCALSLVGEPIMYPEIN LVDELH RRI
Sbjct: 365 KGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELHGRRI 424

Query: 424 STFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEK 483
           STFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKAL EK
Sbjct: 425 STFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKALQEK 484

Query: 484 QQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSD 543
           QQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVPWH+D
Sbjct: 485 QQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVPWHTD 544

Query: 544 VKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKP 603
           VK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDY+KFHDLVASG+P
Sbjct: 545 VKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVASGEP 604

Query: 604 FDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKP 639
           F S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 FTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

[Close]

BLAST of Cucsa.232190.1 vs. NCBI nr
Match: gi|449469983|ref|XP_004152698.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus])

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 640/640 (100.00%), Postives = 640/640 (100.00%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of Cucsa.232190.1 vs. NCBI nr
Match: gi|659087930|ref|XP_008444711.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 626/641 (97.66%), Postives = 636/641 (99.22%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MS SSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTL SRKPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           AEDFRVGSLLLSQCKFSVFGVGSRVYGET+NAVARDFSKR+K+LGAKEILPIGEGDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEED-EEEDDDLGEEDIVDL 240
           DIDKCFDDWSRKLLKV+K DAEGNGVELCSGIAGDSDAVSVEE+ EEEDDDLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of Cucsa.232190.1 vs. NCBI nr
Match: gi|743832999|ref|XP_011024424.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Populus euphratica])

HSP 1 Score: 1069.7 bits (2765), Expect = 2.0e-309
Identity = 516/642 (80.37%), Postives = 578/642 (90.03%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60
           MSL+S  A+LT+LALLSA+T+YCF KSRRL+K+ L  NP     KPKLFFISQTGTSK L
Sbjct: 1   MSLTSTPARLTLLALLSATTIYCFCKSRRLKKISLKPNPN--PNKPKLFFISQTGTSKTL 60

Query: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRL N+L SN L FDL+DP++YEPEDLFKE LV+ +ASTWEDG PP +AKF ANWL+ES
Sbjct: 61  AHRLHNLLASNNLHFDLIDPQNYEPEDLFKEKLVIIIASTWEDGKPPQNAKFFANWLAES 120

Query: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180
           A+DFRVGSL+LS+CKF+VFGVGSR +GETFNAVA+DFS+RL+ LGAKE++ +GEGDVDGG
Sbjct: 121 ADDFRVGSLMLSECKFAVFGVGSRAFGETFNAVAKDFSRRLRDLGAKEVVGVGEGDVDGG 180

Query: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEEDIVDLE 240
           ++D  F++WS +L +V+K ++  NG E+C    G+   V  E D++EDD   E DI+DLE
Sbjct: 181 ELDGVFEEWSGRLARVLKGESVENG-EVC----GNGLVVIDESDDDEDDGGVESDIIDLE 240

Query: 241 DIAGKGPSRKSTNIVETNGKLNGK-------KVMVTPVIRASLEKQGYKIIGSHSGVKIC 300
           DIAGKGPSR+S  + ++NGKLNG+       K MVTPVIRASLEKQGYKIIGSHSGVKIC
Sbjct: 241 DIAGKGPSRRSIVVDQSNGKLNGENGKLNGQKEMVTPVIRASLEKQGYKIIGSHSGVKIC 300

Query: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 360
           RWTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MDD
Sbjct: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDD 360

Query: 361 PLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 420
           PLEIVNSAIDLHTKMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPEINTLVDEL
Sbjct: 361 PLEIVNSAIDLHTKMIKQMKGVPGVTLERLNEGLSPRHCALSLVGEPIMYPEINTLVDEL 420

Query: 421 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 480
           HRR+ISTFLVTNAQFP+KI++LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK
Sbjct: 421 HRRQISTFLVTNAQFPEKIQMLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 480

Query: 481 ALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENV 540
           +LNEKQQRTVYRLTLVKGWNTED+DAY NLFS+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 481 SLNEKQQRTVYRLTLVKGWNTEDVDAYFNLFSVGQPDFIEIKGVTYCGSSATSKLTMENV 540

Query: 541 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLV 600
           PWHSDVK+FSEALA KS+GEYEVACEHVHSCCVLLAKT+KF+VDGQWFTWIDYDKFHDLV
Sbjct: 541 PWHSDVKAFSEALAFKSKGEYEVACEHVHSCCVLLAKTEKFKVDGQWFTWIDYDKFHDLV 600

Query: 601 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 636
           ASGKPFDSKDYMA TPSWAVYG++EGGFDPDQSRYRKERHHK
Sbjct: 601 ASGKPFDSKDYMAATPSWAVYGAEEGGFDPDQSRYRKERHHK 635

[Close]

BLAST of Cucsa.232190.1 vs. NCBI nr
Match: gi|645228029|ref|XP_008220799.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Prunus mume])

HSP 1 Score: 1064.7 bits (2752), Expect = 6.4e-308
Identity = 518/641 (80.81%), Postives = 573/641 (89.39%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPS--RKPKLFFISQTGTSK 60
           MSLSS  A+LT+LALLSA+T YCFYKSRRL++LKLSLNP   S  RK KLFFISQTGTSK
Sbjct: 1   MSLSSVPARLTLLALLSATTFYCFYKSRRLKQLKLSLNPNPSSSPRKGKLFFISQTGTSK 60

Query: 61  ALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLS 120
           ALAHRLL++L SNGL FDLVDP  YEPEDL KETLVL +ASTW+DG PP +AKF +NWL+
Sbjct: 61  ALAHRLLDLLTSNGLGFDLVDPNHYEPEDLPKETLVLIIASTWDDGKPPANAKFFSNWLA 120

Query: 121 ESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVD 180
           ESAEDFRVGSLLLS CKF+VFGVGSR YG TFNAV +D +KR++ALGA EI  I EGDVD
Sbjct: 121 ESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAKRMRALGAAEIFSIWEGDVD 180

Query: 181 GGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVE---EDEEEDDDLGEED 240
           GGDID+ F+ WS K+L+ +K     NG  L +G+  + +A S +   ED+EE+DDL E +
Sbjct: 181 GGDIDEVFEAWSGKVLRFLKGGVAENGGVLSNGVGAECEAESFDGSDEDDEEEDDL-ESE 240

Query: 241 IVDLEDIAGKGPSRKST-NIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 300
           +VDLEDIAGKGPSR+ T  + +TNG +NG+K MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 241 VVDLEDIAGKGPSRRRTVTVTKTNGTVNGQKEMVTPVIRASLVKQGYKIIGSHSGVKICR 300

Query: 301 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 360
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MDDP
Sbjct: 301 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDDP 360

Query: 361 LEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELH 420
           LEIVNSAIDLHTKMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 361 LEIVNSAIDLHTKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPIMYPEINALVDELH 420

Query: 421 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 480
           RRRISTFLVTNAQFP+KIK+LKP+TQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK+
Sbjct: 421 RRRISTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKS 480

Query: 481 LNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVP 540
           L EKQQRTVYRLTLVKGWNTEDIDAY N+FSIG PDFVEIKGVTYCGSS TSKLTMENVP
Sbjct: 481 LKEKQQRTVYRLTLVKGWNTEDIDAYYNIFSIGNPDFVEIKGVTYCGSSATSKLTMENVP 540

Query: 541 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVA 600
           WH+DVK+FSEALA KS GEYEVACEHVHSCCVLLAKTDKF+++GQWFTWIDY+KFHDLV+
Sbjct: 541 WHADVKAFSEALALKSNGEYEVACEHVHSCCVLLAKTDKFKINGQWFTWIDYEKFHDLVS 600

Query: 601 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 636
           SG+PFDSKDYMA TPSWAVYG++EGGFDP QSRY+KERHH+
Sbjct: 601 SGRPFDSKDYMAATPSWAVYGAEEGGFDPYQSRYKKERHHR 640

[Close]

BLAST of Cucsa.232190.1 vs. NCBI nr
Match: gi|1009134935|ref|XP_015884714.1| (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Ziziphus jujuba])

HSP 1 Score: 1056.2 bits (2730), Expect = 2.3e-305
Identity = 517/642 (80.53%), Postives = 566/642 (88.16%), Query Frame = 1


Query: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNP-----TLPSRKPKLFFISQTG 60
           MS SS   +LT+LALLSA+T YCFYKSRRL+ L  SLNP     +   RK KLFFISQTG
Sbjct: 4   MSFSSFPTRLTLLALLSATTFYCFYKSRRLKLLSQSLNPNGKTSSSSPRKAKLFFISQTG 63

Query: 61  TSKALAHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLAN 120
           TSKALA RL ++L SN L FDLVDP  YEPEDL KE LVL VASTWEDG  PP AKF AN
Sbjct: 64  TSKALAVRLFDLLTSNNLAFDLVDPNHYEPEDLPKENLVLIVASTWEDGKAPPTAKFFAN 123

Query: 121 WLSESAEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEG 180
           WLSES++DFRVGSLLLSQCKF+VFGVGSR YG TFNAVA+D SK+++ALGAKEILP+ EG
Sbjct: 124 WLSESSDDFRVGSLLLSQCKFAVFGVGSRAYGATFNAVAKDVSKQMRALGAKEILPVWEG 183

Query: 181 DVDGGDIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEEDEEEDDDLGEED 240
           DVDGGDID+ FD WS K++   K     NG    +G+  +SDA S +E +EE+  + E +
Sbjct: 184 DVDGGDIDEVFDAWSGKVVGFCKGGVVENGGVSTNGVDCESDACSGDETDEEEYSM-ESE 243

Query: 241 IVDLEDIAGKGPSRKSTNIV--ETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKIC 300
           IVDLEDIAGKGPSR+S  +   ETNG LNGKK MVTPVIRASLEKQGYKIIGSHSGVKIC
Sbjct: 244 IVDLEDIAGKGPSRRSATVTKAETNGTLNGKKDMVTPVIRASLEKQGYKIIGSHSGVKIC 303

Query: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 360
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW+MDD
Sbjct: 304 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDD 363

Query: 361 PLEIVNSAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDEL 420
           PLEIVNSAI+ HTKMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPEINTLVDEL
Sbjct: 364 PLEIVNSAIEQHTKMIKQMKGVPGVTVERLREGLSPRHCALSLVGEPIMYPEINTLVDEL 423

Query: 421 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 480
           HRRR+STFLVTNAQFP+KIK+LKP+TQLYVSVDAATKESLKAIDRPLFGDFWER IDSLK
Sbjct: 424 HRRRVSTFLVTNAQFPEKIKMLKPITQLYVSVDAATKESLKAIDRPLFGDFWERLIDSLK 483

Query: 481 ALNEKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENV 540
           AL EKQQRTVYRLTLVKGWNTEDI+AYSNLFSIG+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 484 ALKEKQQRTVYRLTLVKGWNTEDIEAYSNLFSIGKPDFIEIKGVTYCGSSATSKLTMENV 543

Query: 541 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLV 600
           PWHSDV+ FSEALA +S GEYEVACEHVHSCCVLLAKTDKF+++GQWFTWIDY+KFHDLV
Sbjct: 544 PWHSDVRDFSEALALRSLGEYEVACEHVHSCCVLLAKTDKFKINGQWFTWIDYEKFHDLV 603

Query: 601 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHK 636
           ASG+PFDSKDYMA TPSWAVYG++EGGFDPDQSRY+KERHH+
Sbjct: 604 ASGRPFDSKDYMAITPSWAVYGAEEGGFDPDQSRYKKERHHR 644

The following BLAST results are available for this feature:

BLAST of Cucsa.232190.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TYW1_ARATH	2.5e-280	73.13	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
TYW1_ORYSJ	1.0e-257	68.33	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
TYW1_XENLA	4.0e-177	50.00	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Xenopus lae...	[more]
TYW1_DANRE	2.3e-172	48.38	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Danio rerio...	[more]
TYW1_MOUSE	3.4e-160	46.79	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Mus musculu...	[more]

BLAST of Cucsa.232190.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPA4_CUCSA	0.0e+00	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G361620 PE=4 SV=1	[more]
A0A067L1Y3_JATCU	2.8e-302	78.36	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04425 PE=4 SV=1	[more]
A0A061FDJ1_THECC	4.5e-300	79.25	Flavodoxin family protein / radical SAM domain-containing protein isoform 1 OS=T...	[more]
F6H045_VITVI	1.7e-299	78.33	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g12460 PE=4 SV=...	[more]
W9RQ06_9ROSA	7.9e-297	73.41	tRNA wybutosine-synthesizing protein 1-like protein OS=Morus notabilis GN=L484_0...	[more]

BLAST of Cucsa.232190.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G75200.1	1.4e-281	73.13	flavodoxin family protein / radical SAM domain-containing protein	[more]

BLAST of Cucsa.232190.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449469983\|ref\|XP_004152698.1\|	0.0e+00	100.00	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
gi\|659087930\|ref\|XP_008444711.1\|	0.0e+00	97.66	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
gi\|743832999\|ref\|XP_011024424.1\|	2.0e-309	80.37	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Po...	[more]
gi\|645228029\|ref\|XP_008220799.1\|	6.4e-308	80.81	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Pr...	[more]
gi\|1009134935\|ref\|XP_015884714.1\|	2.3e-305	80.53	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Zi...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001094	Flavdoxin-like
IPR007197	rSAM
IPR008254	Flavodoxin/NO_synth
IPR013785	Aldolase_TIM
IPR013917	tRNA_wybutosine-synth

Vocabulary: **Molecular Function**
Term	Definition
GO:0010181	FMN binding
GO:0003824	catalytic activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0032259	methylation
cellular_component	GO:0005768	endosome
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005802	trans-Golgi network
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003958	NADPH-hemoprotein reductase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.232190	Cucsa.232190	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.232190.1	Cucsa.232190.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232190.1.five_prime_UTR.1	Cucsa.232190.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232190.1.CDS.1	Cucsa.232190.1.CDS.1	CDS
Cucsa.232190.1.CDS.2	Cucsa.232190.1.CDS.2	CDS
Cucsa.232190.1.CDS.3	Cucsa.232190.1.CDS.3	CDS
Cucsa.232190.1.CDS.4	Cucsa.232190.1.CDS.4	CDS
Cucsa.232190.1.CDS.5	Cucsa.232190.1.CDS.5	CDS
Cucsa.232190.1.CDS.6	Cucsa.232190.1.CDS.6	CDS
Cucsa.232190.1.CDS.7	Cucsa.232190.1.CDS.7	CDS
Cucsa.232190.1.CDS.8	Cucsa.232190.1.CDS.8	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.232190.1.three_prime_UTR.1	Cucsa.232190.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 95..106 score: 3.2E-12coord: 133..143 score: 3.2E-12coord: 158..177 score: 3.2E-12coord: 47..60 score: 3.2
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 324..502 score: 1.6
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 score: 1.4
IPR008254	Flavodoxin/nitric oxide synthase	PROFILE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 22
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 327..514 score: 2.0
IPR013917	tRNA wybutosine-synthesis	PFAM	PF08608	Wyosine_form	coord: 507..570 score: 9.9
None	No IPR available	PANTHER	PTHR13930	RSAFD1-RELATED	coord: 38..637 score:
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 38..637 score:
None	No IPR available	unknown	SSF102114	Radical SAM enzymes	coord: 325..504 score: 1.96