Cucsa.232030.3 (mRNA) Cucumber (Gy14) v1

NameCucsa.232030.3
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold01989 : 169411 .. 174302 (+)
Sequence length2974
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAAGGTTGCTCTTTTCCTAAACCCTACTTCAGTTCAGTCACCCCCATATTACCATTGGTACCCACTGCCTTCCCCGCGCTGTTCGTTCCTGCCGCCGCCTCACCGGTCACTGCCGCAGCACTCTCCGCCTTCCAACGAAATTGTTGTGACCTCTCCTTTTCTAATTCTCTCGCCCCTCCGACCAATCTAAGTTAATTATCTTTGGTTTTTtCTTCTCAGAAACACAGTGGTTCAGCCCCTAACCACTTCCGTTTGTGAGATCGCTCTGCATCAGCTCATTTTTCAGGTCCCAATGCTTTATTTTTTGCGTTTATATATATTTTTGTTTTGCTCGGTTTTGTTGGTTTATACGCTTCACAGAAGAAAATCAATGGTTGATTTGTTGTTCAACCCACGTTCCACTAGTCTTAATGAAAGCTTAAAACTCAGAATCTTTGCCTCACAAGTTAGAAGAAGAAAAGGGAATTAAATCAATTAATGAATGGGGAACAAATTGCCTAGCATTCTTATCCGAATCAATAAACAAAGAGAAAAGAAAGATGTATATGGTTTGGTATGAAAATCTTGTTGAAAGATAACTGTATTTGAAAAAGTTGAAACAATCGTGATCAATTGAAATGAACAACAAAATACAAGGGAAACAAGATAGAAAACTAACTTTGAGTTTGAGTTTAATTGAAGGAATTGAGACACAGGGAGCTGCTGTTGGGAACGGTTTTGTTGTTGTTGTTTGTTTATTTGTTTTTTTTTTTtCCTGTTTCCTTCCTGTTTACAATCTTGGAAAATCCAATTTTTTTtCCTCATTTGCAACCAGGGGTTCCAAACAAGGCAAGGGACCAAAGATGAGCTTAAAGCATTTACTCCTGCGTCAAACACGGAAGAATTTTTCAAAAATCAATGGCAACTTACTGGATCGGCAATCACCTAACATAAATGCCACTCACATATTCATCACAAAACCCTCGTTTtCTTTGCTTGATTCACACCGTGGTTATTATTCATCACTTGCTGCTAGAAATTTTGAGCTCAGCAAATTGAATTCACTTTTCAGTAGGTGCATTCACTTCACTGTAACCAAGTTGAGTAATGCAGCAATTGAGCCAAAACCTGAATCTGCAGAAGTTGAGGATGATGATGGATCAATGAACGAGTTCTTATCCAGATTTGTTTGGATAATGCGCGAGAAGATTTCTGAAGAATTTCCGGATTACGATAAGCAAACAGTTAATGCAATGCTTTTGATGATAGTGGAAAAAGTAGTATCTGAAATGGAAAAGGGTAGGTTTGAGCAGACGCTAAAGGCTTCAACTGATAATCCAGATTGGGACCTAAGTGAGGATTTGTGGAAGACGGTAACTGAAGTTAGCAACATGGTTTTGGATGATATGAAGAAGGCTACAAAGAAGGAGAAAATGAAGGGTTTTCTCCTGTCCAGGGAAGTTCAGGAAATGTGTAGGTTTGCTGGGGAAGTTGGTATTCGAGGGGATATGCTGAGGGAATTTAGGTTCAAATGGGCCCGTGAGAAAATGGAGGAAAGTGAGTTTTACGAGAGTCTGGAGCAGCTCAGAAAAGAGGCACGTACCCAGGAAGAAAaCAAGGATTCTGCAAGTGGTGCAGAGGCTGCATCTGAGGTGAAATCCGAGGCTGTTTCTCTTCCCAAAAGGCGAGGGAAGTTAAAGTACAAGATTTATGGTCTTGATTTATCTGATCCTAAGTGGAGTGAATTAGCAGACAAATTACATGTGGCAGAGAAGCTGATATTGCCGCAGGAACCAAAGCCAATTTCTGGGATGTGCAAACTGGTCACAGAGAAAATTCTTTTGTTAAATGAGAACGACGACCCATCTCCATTATTGGCTGAATGGAAAGAGCTTCTTCAACCCACTAGGATTGACTGGATTACCTTACTTGATAGGTTGAATGAGAAGAATAGATTCTTATACTTCAAGGTAAAACAGCATCTTTTATTTCCCACTCTTGCCTTGCGTTATAGTACAATTTTGATTGTTTGTTTCAAAATTCACTCAGTTACCCTATATATATACTTGTTGCACACATTAAATAGACTTGTGTTGTCAATGCAAACCATGTAGAATCCTATCAAGATATATCTTGCAGATCAACATTTTTAATTTCAGAAACAAATAATTTTTACTTGTGTAACGATCTAGTATATGTGCTTAACAATCAATTATTGGAGTAGAAGCTGCTGTTTTCCACGGAGGATTGGTATAAACGAAGGAGAAGATTGTTGTCGTTTTTTTCTTTTtCCACTTACCTTATGTTGTTTTtCTTAATTGTTATTGCTTAATGTTTATGTGTCCTGAACAGAGGAGAGGAAATTTCATTTATCCATTGTATTAATAGTTAGAATTGGTGCTTGCTTTATGAGATCTTGGTTGCTTTTGTTTGGATTGTCATATATTTGGCGTGAAGTTGGCCTCTGCTCTTTTGATGGTGTTATGCAATGGGTGTGTGTATCAATGAAACTTTTCTACTGCATATTATAAAGAACGATTTAATGTTAGCAATAAGACCCTAATTCAAACTTGTTGCTTATTATGAATGAAGGTAGCAGAACTTCTTTTGAGTGAAGAGTCTTTCCAGACCAACATCCGTGACTACTCTAAGCTTATTGAGGTCTATGCTAAAGAGAATCGCCTAGAGGATGCTGAGAGGATTCTTGTCAAGATGAATGAGAAGGGCATTGCACCGGACATATTGACAACCATATATTTGATTCATATGTATAGCAAGGCAGGCAATCTTGATAGTGCGAAGAAAGCTTTTGATTCCTTGAGGAGTCACGGCTTCCAACCAGATGAGAAGGTATATAATTCCATGATAATGGCATATGTAAATGCTGGACAACCCAAGTTGGGGGAGTCACTGATGAGAGATATGGAAGCAAGGGACATCAAACCCAGCCAGGACATTTACATGGCATTACTTAGATCATTTTCCCAATGTGGTTATGTTAGTGGCGCTGGAAGAATTGCTGCTACTATGCAGTTTGCTGGCATCTCTCCAAATTTGGAATCATGTACATTGCTCGTCGAGGCATATGGGCAAGCTGGCGATCCTGATAAGGCAAGGAACAATTTTGACTACATGATAAAACTTGGGCACGCACCTGATGACAGGTGCACTGCAAGTATGATTGCAGCCTATGAAAAGAAGAATCTGTTGGACAAGGCTTTGGATCTTTTACTACAACTTGAAAAGGATGGCTTTGAGCCTGGGCTTGCAACTTATGCTGTTCTTGTAGATTGGTTGGGTAAGTTGCAGCTGGTTGAAGAAGCCGAGCAGGTATTAGCCAAGATTGGCGCACAGGGTCACTATCTCCCCATTAAGGTTCGTATTAGCCTATGTGATATGTACTCAAGAGCTGGGATTGAGAAAAAGGCGCTGCAAGCTCTGAAGATCTTGGAAGCTAAAAAGCAGGAGTTGGGACATGATGATTTTGAAAGGATCATAAATGGACTTGTAGCAGGTGGCTTTTTGCAGGATGCTAAAAGGATGGAGGGTGTTATGGAGGCCCAGGGTTTTATTGCTTCCCAACCTCTTCAAATGGCTCTGAGGACTTCTCAAGCTCTTCGTGGCAAAAGACTACCTTGAGATGATTTTTTGATAGTTCATAAATTTATCGACAATTCATTGCTGCTGGCGGAAATGTGAATATTTTACCATCAACTTGAATTCTGTGCAAAGCTAGTGGCTAGAAAGATTTTGTAGGATGGTATGGGATCTCTATGGAAATAATTGGCATCTCAGCATTGCTTTGTGAGGAATTGTTATTTCCCCCAACTGATTATAGGTTAAACCCCTCTCTAGTTTTCTTTAACCATGCTATATTCTTGGTTGCATATACCTTACGAGTAGTATTTTTCATGAAGTTAATAAGATAAGAATACATGGAAAAAAACTATTATTCATTGACCACTAAATAAGGACCTGGAGGGAACGGATTTAATCCAGTCACCTACCTCTTGCGAGTTTCCTGGCTAACCAATATTTTAGGGTCGTGTGCATACTCACATATATATAAAAATAAAATAAAATAAAATATAAAGATTTCACACTATGCTTTGACTTCTTTTAAATCTTTTTCCTTCTTTTGCTTTTACATGGTATCCATTGTCGGGCTGTCTCTCAATAAAGAGTGTTTCTGTTGCATGAATTGTGTATTTTCTGATTCTTAGATTTGAGTTTTAATAATATTCTCCTTTTATATAGTTGTTTAGTTGCTCAAGTTCCCCATTTTGCTGCTTTACAACTGCTCTGGCAACATCTACAATAGAAATCATGCCAACTAGTTTTCCATCTATCACAGGAATATGTCTTATGTGATTCTCTGCAAATGCCAAAAACTTCAACTGGATTATTCTCCTCTTGGATGATCGTTGATGCAGCTGAAGAAGGAGCTGACTTCCCTTTTTGTAATGGTAATTTCAGAATCTAAATTAAACTACATTTAGTATTTACCTGTCATAAGCTGCATTGCTTTAAGGATGTTCGTGTCCGACGTTATGGTTACTAGTTTGTCCTGTTGATGGAAAATAAAAaTGTATGCTTGAGAACTGTTGTTTGTAAACTGGTTTTGTAGCCAAGGGAAGACAGAAAATAACCTCATGAGTCATTATTTCTCCAACTTTTGTGTATATGGGAGATCTCCCATCTGCTATTATTTTCTTCAGGTAGTCTGCTTAGGATCATCAGAAATCAGCAGTCACCAAGTTGATGATATTCATGGAATAGAGCTCAAAGGAGAACCTACTTTCTTCCTTTTCCTTCCCCGAAAAACTACCCTTGGATCTTTAGGTTTCAAAATGCTACGTACTTACCCCACTTTAATTTTAAGGTTTGAGTTCATTTTCAATTTATCTGTAG

mRNA sequence

ATCAAGGTTGCTCTTTTCCTAAACCCTACTTCAGTTCAGTCACCCCCATATTACCATTGGTACCCACTGCCTTCCCCGCGCTGTTCGTTCCTGCCGCCGCCTCACCGGTCACTGCCGCAGCACTCTCCGCCTTCCAACGAAATTGTTGTGACCTCTCCTTTTCTAATTCTCTCGCCCCTCCGACCAATCTAAGTTAATTATCTTTGGTTTTTTCTTCTCAGAAACACAGTGGTTCAGCCCCTAACCACTTCCGTTTGTGAGATCGCTCTGCATCAGCTCATTTTTCAGGGGTTCCAAACAAGGCAAGGGACCAAAGATGAGCTTAAAGCATTTACTCCTGCGTCAAACACGGAAGAATTTTTCAAAAATCAATGGCAACTTACTGGATCGGCAATCACCTAACATAAATGCCACTCACATATTCATCACAAAACCCTCGTTTTCTTTGCTTGATTCACACCGTGGTTATTATTCATCACTTGCTGCTAGAAATTTTGAGCTCAGCAAATTGAATTCACTTTTCAGTAGGTGCATTCACTTCACTGTAACCAAGTTGAGTAATGCAGCAATTGAGCCAAAACCTGAATCTGCAGAAGTTGAGGATGATGATGGATCAATGAACGAGTTCTTATCCAGATTTGTTTGGATAATGCGCGAGAAGATTTCTGAAGAATTTCCGGATTACGATAAGCAAACAGTTAATGCAATGCTTTTGATGATAGTGGAAAAAGTAGTATCTGAAATGGAAAAGGGTAGGTTTGAGCAGACGCTAAAGGCTTCAACTGATAATCCAGATTGGGACCTAAGTGAGGATTTGTGGAAGACGGTAACTGAAGTTAGCAACATGGTTTTGGATGATATGAAGAAGGCTACAAAGAAGGAGAAAATGAAGGGTTTTCTCCTGTCCAGGGAAGTTCAGGAAATGTGTAGGTTTGCTGGGGAAGTTGGTATTCGAGGGGATATGCTGAGGGAATTTAGGTTCAAATGGGCCCGTGAGAAAATGGAGGAAAGTGAGTTTTACGAGAGTCTGGAGCAGCTCAGAAAAGAGGCACGTACCCAGGAAGAAAACAAGGATTCTGCAAGTGGTGCAGAGGCTGCATCTGAGGTGAAATCCGAGGCTGTTTCTCTTCCCAAAAGGCGAGGGAAGTTAAAGTACAAGATTTATGGTCTTGATTTATCTGATCCTAAGTGGAGTGAATTAGCAGACAAATTACATGTGGCAGAGAAGCTGATATTGCCGCAGGAACCAAAGCCAATTTCTGGGATGTGCAAACTGGTCACAGAGAAAATTCTTTTGTTAAATGAGAACGACGACCCATCTCCATTATTGGCTGAATGGAAAGAGCTTCTTCAACCCACTAGGATTGACTGGATTACCTTACTTGATAGGTTGAATGAGAAGAATAGATTCTTATACTTCAAGGTAGCAGAACTTCTTTTGAGTGAAGAGTCTTTCCAGACCAACATCCGTGACTACTCTAAGCTTATTGAGGTCTATGCTAAAGAGAATCGCCTAGAGGATGCTGAGAGGATTCTTGTCAAGATGAATGAGAAGGGCATTGCACCGGACATATTGACAACCATATATTTGATTCATATGTATAGCAAGGCAGGCAATCTTGATAGTGCGAAGAAAGCTTTTGATTCCTTGAGGAGTCACGGCTTCCAACCAGATGAGAAGGTATATAATTCCATGATAATGGCATATGTAAATGCTGGACAACCCAAGTTGGGGGAGTCACTGATGAGAGATATGGAAGCAAGGGACATCAAACCCAGCCAGGACATTTACATGGCATTACTTAGATCATTTTCCCAATGTGGTTATGTTAGTGGCGCTGGAAGAATTGCTGCTACTATGCAGTTTGCTGGCATCTCTCCAAATTTGGAATCATGTACATTGCTCGTCGAGGCATATGGGCAAGCTGGCGATCCTGATAAGGCAAGGAACAATTTTGACTACATGATAAAACTTGGGCACGCACCTGATGACAGGTGCACTGCAAGTATGATTGCAGCCTATGAAAAGAAGAATCTGTTGGACAAGGCTTTGGATCTTTTACTACAACTTGAAAAGGATGGCTTTGAGCCTGGGCTTGCAACTTATGCTGTTCTTGTAGATTGGTTGGGTAAGTTGCAGCTGGTTGAAGAAGCCGAGCAGGTATTAGCCAAGATTGGCGCACAGGGTCACTATCTCCCCATTAAGGTTCGTATTAGCCTATGTGATATGTACTCAAGAGCTGGGATTGAGAAAAAGGCGCTGCAAGCTCTGAAGATCTTGGAAGCTAAAAAGCAGGAGTTGGGACATGATGATTTTGAAAGGATCATAAATGGACTTGTAGCAGGTGGCTTTTTGCAGGATGCTAAAAGGATGGAGGGTGTTATGGAGGCCCAGGGTTTTATTGCTTCCCAACCTCTTCAAATGGCTCTGAGGACTTCTCAAGCTCTTCGTGGCAAAAGACTACCTTGAGATGATTTTTTGATAGTTCATAAATTTATCGACAATTCATTGCTGCTGGCGGAAATGTGAATATTTTACCATCAACTTGAATTCTGTGCAAAGCTAGTGGCTAGAAAGATTTTGTAGGATGGTATGGGATCTCTATGGAAATAATTGGCATCTCAGCATTGCTTTGTGAGGAATTGTTATTTCCCCCAACTGATTATAGGAATATGTCTTATGTGATTCTCTGCAAATGCCAAAAACTTCAACTGGATTATTCTCCTCTTGGATGATCGTTGATGCAGCTGAAGAAGGAGCTGACTTCCCTTTTTGTAATGGTAGTCTGCTTAGGATCATCAGAAATCAGCAGTCACCAAGTTGATGATATTCATGGAATAGAGCTCAAAGGAGAACCTACTTTCTTCCTTTTCCTTCCCCGAAAAACTACCCttggatctttaggtttcaaaatgctacgtacttaccccactttaattttaaggtttgagttcattttcaatttatctgtag

Coding sequence (CDS)

ATGAGCTTAAAGCATTTACTCCTGCGTCAAACACGGAAGAATTTTTCAAAAATCAATGGCAACTTACTGGATCGGCAATCACCTAACATAAATGCCACTCACATATTCATCACAAAACCCTCGTTTtCTTTGCTTGATTCACACCGTGGTTATTATTCATCACTTGCTGCTAGAAATTTTGAGCTCAGCAAATTGAATTCACTTTTCAGTAGGTGCATTCACTTCACTGTAACCAAGTTGAGTAATGCAGCAATTGAGCCAAAACCTGAATCTGCAGAAGTTGAGGATGATGATGGATCAATGAACGAGTTCTTATCCAGATTTGTTTGGATAATGCGCGAGAAGATTTCTGAAGAATTTCCGGATTACGATAAGCAAACAGTTAATGCAATGCTTTTGATGATAGTGGAAAAAGTAGTATCTGAAATGGAAAAGGGTAGGTTTGAGCAGACGCTAAAGGCTTCAACTGATAATCCAGATTGGGACCTAAGTGAGGATTTGTGGAAGACGGTAACTGAAGTTAGCAACATGGTTTTGGATGATATGAAGAAGGCTACAAAGAAGGAGAAAATGAAGGGTTTTCTCCTGTCCAGGGAAGTTCAGGAAATGTGTAGGTTTGCTGGGGAAGTTGGTATTCGAGGGGATATGCTGAGGGAATTTAGGTTCAAATGGGCCCGTGAGAAAATGGAGGAAAGTGAGTTTTACGAGAGTCTGGAGCAGCTCAGAAAAGAGGCACGTACCCAGGAAGAAAaCAAGGATTCTGCAAGTGGTGCAGAGGCTGCATCTGAGGTGAAATCCGAGGCTGTTTCTCTTCCCAAAAGGCGAGGGAAGTTAAAGTACAAGATTTATGGTCTTGATTTATCTGATCCTAAGTGGAGTGAATTAGCAGACAAATTACATGTGGCAGAGAAGCTGATATTGCCGCAGGAACCAAAGCCAATTTCTGGGATGTGCAAACTGGTCACAGAGAAAATTCTTTTGTTAAATGAGAACGACGACCCATCTCCATTATTGGCTGAATGGAAAGAGCTTCTTCAACCCACTAGGATTGACTGGATTACCTTACTTGATAGGTTGAATGAGAAGAATAGATTCTTATACTTCAAGGTAGCAGAACTTCTTTTGAGTGAAGAGTCTTTCCAGACCAACATCCGTGACTACTCTAAGCTTATTGAGGTCTATGCTAAAGAGAATCGCCTAGAGGATGCTGAGAGGATTCTTGTCAAGATGAATGAGAAGGGCATTGCACCGGACATATTGACAACCATATATTTGATTCATATGTATAGCAAGGCAGGCAATCTTGATAGTGCGAAGAAAGCTTTTGATTCCTTGAGGAGTCACGGCTTCCAACCAGATGAGAAGGTATATAATTCCATGATAATGGCATATGTAAATGCTGGACAACCCAAGTTGGGGGAGTCACTGATGAGAGATATGGAAGCAAGGGACATCAAACCCAGCCAGGACATTTACATGGCATTACTTAGATCATTTTCCCAATGTGGTTATGTTAGTGGCGCTGGAAGAATTGCTGCTACTATGCAGTTTGCTGGCATCTCTCCAAATTTGGAATCATGTACATTGCTCGTCGAGGCATATGGGCAAGCTGGCGATCCTGATAAGGCAAGGAACAATTTTGACTACATGATAAAACTTGGGCACGCACCTGATGACAGGTGCACTGCAAGTATGATTGCAGCCTATGAAAAGAAGAATCTGTTGGACAAGGCTTTGGATCTTTTACTACAACTTGAAAAGGATGGCTTTGAGCCTGGGCTTGCAACTTATGCTGTTCTTGTAGATTGGTTGGGTAAGTTGCAGCTGGTTGAAGAAGCCGAGCAGGTATTAGCCAAGATTGGCGCACAGGGTCACTATCTCCCCATTAAGGTTCGTATTAGCCTATGTGATATGTACTCAAGAGCTGGGATTGAGAAAAAGGCGCTGCAAGCTCTGAAGATCTTGGAAGCTAAAAAGCAGGAGTTGGGACATGATGATTTTGAAAGGATCATAAATGGACTTGTAGCAGGTGGCTTTTTGCAGGATGCTAAAAGGATGGAGGGTGTTATGGAGGCCCAGGGTTTTATTGCTTCCCAACCTCTTCAAATGGCTCTGAGGACTTCTCAAGCTCTTCGTGGCAAAAGACTACCTTGA

Protein sequence

MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNFELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEFPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLDDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQLRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP*
BLAST of Cucsa.232030.3 vs. Swiss-Prot
Match: NFD5_ARATH (Protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial OS=Arabidopsis thaliana GN=NFD5 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.8e-93
Identity = 187/360 (51.94%), Postives = 247/360 (68.61%), Query Frame = 1

Query: 21  NLLDRQSPNINATHIFITKPSFSLLDSH-RGYYSSLAARNFELSKLNSLFSRCIHFTVTK 80
           +LL  ++P        IT  + S  D H R Y      +N E+ +  S F+R  HFT   
Sbjct: 15  SLLSSKTPTFCRNFSAITS-TISHSDRHLRSYDEQTPFQNVEIPRPISSFNRYFHFTRES 74

Query: 81  L---SNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEFPDYDKQTVNAMLLMIV 140
               S+AAI+   +  E +D+DG+ NEFLSRFVWIMR K+SE +PD DK+ ++ MLL+IV
Sbjct: 75  RLSESSAAIDDSNDQEE-DDEDGTTNEFLSRFVWIMRGKVSEAYPDCDKKMIDGMLLLIV 134

Query: 141 EKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLDDMKKATKKEKMKGFLL 200
           EKVV E+E+G F + + ++  +P  + S+DLW T+ EVSN VL DM+K  KKEKMK ++ 
Sbjct: 135 EKVVEEIERGGFNK-VGSAPPSPSSEFSDDLWATIWEVSNTVLKDMEKERKKEKMKQYVQ 194

Query: 201 SREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQLR------KEARTQEE 260
           S EV EMCRFAGE+GIRGD+LRE RFKWAREKM+++EFYESLEQ R      +E+ T + 
Sbjct: 195 SPEVMEMCRFAGEIGIRGDLLRELRFKWAREKMDDAEFYESLEQQRDLDNSIRESETVDG 254

Query: 261 NKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLHVAEKLILPQE 320
             +   G   + EV+S ++SLPKR+GKLKYKIYGL+LSDPKW E+ADK+H AE+    +E
Sbjct: 255 EVEE-EGFVPSDEVESRSISLPKRKGKLKYKIYGLELSDPKWVEMADKIHEAEEEADWRE 314

Query: 321 PKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLNEKNRFLYFKV 371
           PKP++G CKLV EK+  L E DDPS LLAEW ELL+P R+DWI L+++L E N   Y KV
Sbjct: 315 PKPVTGKCKLVMEKLESLQEGDDPSGLLAEWAELLEPNRVDWIALINQLREGNTHAYLKV 370

BLAST of Cucsa.232030.3 vs. Swiss-Prot
Match: PPR51_ARATH (Pentatricopeptide repeat-containing protein At1g19525 OS=Arabidopsis thaliana GN=At1g19525 PE=2 SV=2)

HSP 1 Score: 329.7 bits (844), Expect = 7.9e-89
Identity = 160/295 (54.24%), Postives = 226/295 (76.61%), Query Frame = 1

Query: 410 MNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQ 469
           M++ GI PDILT   L+HMYSK+GN + A +AF++L+S+G +PDEK+Y +MI+ YVNAG+
Sbjct: 1   MSQNGIFPDILTATALVHMYSKSGNFERATEAFENLKSYGLRPDEKIYEAMILGYVNAGK 60

Query: 470 PKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISP-NLESCT 529
           PKLGE LM++M+A+++K S+++YMALLR+++Q G  +GA  I+++MQ+A   P + E+ +
Sbjct: 61  PKLGERLMKEMQAKELKASEEVYMALLRAYAQMGDANGAAGISSSMQYASDGPLSFEAYS 120

Query: 530 LLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKD 589
           L VEAYG+AG  DKA++NFD M KLGH PDD+C A+++ AY+ +N LDKAL LLLQLEKD
Sbjct: 121 LFVEAYGKAGQVDKAKSNFDEMRKLGHKPDDKCIANLVRAYKGENSLDKALRLLLQLEKD 180

Query: 590 GFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKA 649
           G E G+ TY VLVDW+  L L+EEAEQ+L KI   G   P ++++SLC MYS    EKK 
Sbjct: 181 GIEIGVITYTVLVDWMANLGLIEEAEQLLVKISQLGEAPPFELQVSLCCMYSGVRNEKKT 240

Query: 650 LQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQM 704
           LQAL +LEAK+ ++G ++F+++I+ L  GGF +DA+RM   MEA+ F+ SQ LQM
Sbjct: 241 LQALGVLEAKRDQMGPNEFDKVISALKRGGFEKDARRMYKYMEARKFLPSQRLQM 295


HSP 2 Score: 55.5 bits (132), Expect = 2.9e-06
Identity = 39/139 (28.06%), Postives = 66/139 (47.48%), Query Frame = 1

Query: 373 LLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKA 432
           L L ++  +  +  Y+ L++  A    +E+AE++LVK+++ G AP     + L  MYS  
Sbjct: 175 LQLEKDGIEIGVITYTVLVDWMANLGLIEEAEQLLVKISQLGEAPPFELQVSLCCMYSGV 234

Query: 433 GNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIY 492
            N     +A   L +   Q     ++ +I A    G  K    + + MEAR   PSQ + 
Sbjct: 235 RNEKKTLQALGVLEAKRDQMGPNEFDKVISALKRGGFEKDARRMYKYMEARKFLPSQRLQ 294

Query: 493 MALLRSFSQCGYVSGAGRI 512
           M ++      G  SG+GR+
Sbjct: 295 MDMVAPPRAFG--SGSGRV 311


HSP 3 Score: 49.7 bits (117), Expect = 1.6e-04
Identity = 39/161 (24.22%), Postives = 64/161 (39.75%), Query Frame = 1

Query: 369 KVAELLLSE---ESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYL 428
           K+ E L+ E   +  + +   Y  L+  YA+      A  I   M      P       L
Sbjct: 62  KLGERLMKEMQAKELKASEEVYMALLRAYAQMGDANGAAGISSSMQYASDGPLSFEAYSL 121

Query: 429 -IHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARD 488
            +  Y KAG +D AK  FD +R  G +PD+K   +++ AY           L+  +E   
Sbjct: 122 FVEAYGKAGQVDKAKSNFDEMRKLGHKPDDKCIANLVRAYKGENSLDKALRLLLQLEKDG 181

Query: 489 IKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLE 526
           I+     Y  L+   +  G +  A ++   +   G +P  E
Sbjct: 182 IEIGVITYTVLVDWMANLGLIEEAEQLLVKISQLGEAPPFE 222


HSP 4 Score: 38.5 bits (88), Expect = 3.6e-01
Identity = 26/136 (19.12%), Postives = 60/136 (44.12%), Query Frame = 1

Query: 388 SKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRS 447
           + L+  Y  EN L+ A R+L+++ + GI   ++T   L+   +  G ++ A++    +  
Sbjct: 155 ANLVRAYKGENSLDKALRLLLQLEKDGIEIGVITYTVLVDWMANLGLIEEAEQLLVKISQ 214

Query: 448 HGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSG 507
            G  P  ++  S+   Y      K     +  +EA+  +   + +  ++ +  + G+   
Sbjct: 215 LGEAPPFELQVSLCCMYSGVRNEKKTLQALGVLEAKRDQMGPNEFDKVISALKRGGFEKD 274

Query: 508 AGRIAATMQFAGISPN 524
           A R+   M+     P+
Sbjct: 275 ARRMYKYMEARKFLPS 290

BLAST of Cucsa.232030.3 vs. Swiss-Prot
Match: PPR1_ARATH (Pentatricopeptide repeat-containing protein At1g01970 OS=Arabidopsis thaliana GN=At1g01970 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.8e-59
Identity = 124/332 (37.35%), Postives = 193/332 (58.13%), Query Frame = 1

Query: 292 WSELADKLHVAEKLILPQEPKPISGMCKLVTEKILLLN-ENDDPSPLLAEWKELLQPTRI 351
           W+++   L   +   + + P  +S  C+ +  +I+  + E      LL  W   + P R 
Sbjct: 72  WADVGLNLTEEQDEAITRIPIKMSKRCQALMRQIICFSPEKGSFCDLLGAWLRRMNPIRA 131

Query: 352 DWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKM 411
           DW+++L  L   +   Y KVAE  L ++SF+ N RDY+K+I  Y K N++EDAER L+ M
Sbjct: 132 DWLSILKELKNLDSPFYIKVAEFSLLQDSFEANARDYTKIIHYYGKLNQVEDAERTLLSM 191

Query: 412 NEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQP 471
             +G   D +T   ++ +YSKAG    A++ F+ ++  G   D + Y SMIMAY+ AG P
Sbjct: 192 KNRGFLIDQVTLTAMVQLYSKAGCHKLAEETFNEIKLLGEPLDYRSYGSMIMAYIRAGVP 251

Query: 472 KLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLL 531
           + GESL+R+M++++I   +++Y ALLR +S  G   GA R+   +Q AGI+P+++ C LL
Sbjct: 252 EKGESLLREMDSQEICAGREVYKALLRDYSMGGDAEGAKRVFDAVQIAGITPDVKLCGLL 311

Query: 532 VEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGF 591
           + AY  +G    AR  F+ M K G    D+C A ++AAYEK+  L++AL  L++LEKD  
Sbjct: 312 INAYSVSGQSQNARLAFENMRKAGIKATDKCVALVLAAYEKEEKLNEALGFLVELEKDSI 371

Query: 592 EPGLATYAVLVDWLGKLQLVEEAEQVLAKIGA 623
             G    AVL  W  KL +VEE E +L +  +
Sbjct: 372 MLGKEASAVLAQWFKKLGVVEEVELLLREFSS 403


HSP 2 Score: 48.5 bits (114), Expect = 3.5e-04
Identity = 29/129 (22.48%), Postives = 59/129 (45.74%), Query Frame = 1

Query: 585 LEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGI 644
           L +D FE     Y  ++ + GKL  VE+AE+ L  +  +G  +      ++  +YS+AG 
Sbjct: 156 LLQDSFEANARDYTKIIHYYGKLNQVEDAERTLLSMKNRGFLIDQVTLTAMVQLYSKAGC 215

Query: 645 EKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMA 704
            K A +    ++   + L +  +  +I   +  G  +  + +   M++Q   A + +  A
Sbjct: 216 HKLAEETFNEIKLLGEPLDYRSYGSMIMAYIRAGVPEKGESLLREMDSQEICAGREVYKA 275

Query: 705 LRTSQALRG 714
           L    ++ G
Sbjct: 276 LLRDYSMGG 284

BLAST of Cucsa.232030.3 vs. Swiss-Prot
Match: PPR12_ARATH (Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidopsis thaliana GN=At1g05670 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.0e-28
Identity = 90/345 (26.09%), Postives = 163/345 (47.25%), Query Frame = 1

Query: 350 IDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVK 409
           I ++  L R+ E +  L      LL+  + +  ++  YS ++  Y +   L+   +++  
Sbjct: 253 IHFVCQLGRIKEAHHLL------LLMELKGYTPDVISYSTVVNGYCRFGELDKVWKLIEV 312

Query: 410 MNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQ 469
           M  KG+ P+      +I +  +   L  A++AF  +   G  PD  VY ++I  +   G 
Sbjct: 313 MKRKGLKPNSYIYGSIIGLLCRICKLAEAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGD 372

Query: 470 PKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTL 529
            +       +M +RDI P    Y A++  F Q G +  AG++   M   G+ P+  + T 
Sbjct: 373 IRAASKFFYEMHSRDITPDVLTYTAIISGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTE 432

Query: 530 LVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDG 589
           L+  Y +AG    A    ++MI+ G +P+     ++I    K+  LD A +LL ++ K G
Sbjct: 433 LINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIG 492

Query: 590 FEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKAL 649
            +P + TY  +V+ L K   +EEA +++ +  A G         +L D Y ++G   KA 
Sbjct: 493 LQPNIFTYNSIVNGLCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQ 552

Query: 650 QALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQG 695
           + LK +  K  +     F  ++NG    G L+D +++   M A+G
Sbjct: 553 EILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKG 591


HSP 2 Score: 92.8 bits (229), Expect = 1.6e-17
Identity = 57/242 (23.55%), Postives = 116/242 (47.93%), Query Frame = 1

Query: 383 NIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAF 442
           N+  Y+ LI+   KE  L+ A  +L +M + G+ P+I T   +++   K+GN++ A K  
Sbjct: 455 NVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNIEEAVKLV 514

Query: 443 DSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQC 502
               + G   D   Y +++ AY  +G+    + ++++M  + ++P+   +  L+  F   
Sbjct: 515 GEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGFCLH 574

Query: 503 GYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCT 562
           G +    ++   M   GI+PN  +   LV+ Y    +   A   +  M   G  PD +  
Sbjct: 575 GMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDGKTY 634

Query: 563 ASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGA 622
            +++  + K   + +A  L  +++  GF   ++TY+VL+    K +   EA +V  ++  
Sbjct: 635 ENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQMRR 694

Query: 623 QG 625
           +G
Sbjct: 695 EG 696


HSP 3 Score: 84.7 bits (208), Expect = 4.4e-15
Identity = 64/275 (23.27%), Postives = 124/275 (45.09%), Query Frame = 1

Query: 387 YSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLR 446
           +++LI  Y K   ++DA R+   M + G +P+++T   LI    K G+LDSA +    + 
Sbjct: 424 FTELINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMW 483

Query: 447 SHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVS 506
             G QP+   YNS++     +G  +    L+ + EA  +      Y  L+ ++ + G + 
Sbjct: 484 KIGLQPNIFTYNSIVNGLCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMD 543

Query: 507 GAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMI 566
            A  I   M   G+ P + +  +L+  +   G  +      ++M+  G AP+     S++
Sbjct: 544 KAQEILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKGIAPNATTFNSLV 603

Query: 567 AAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHY 626
             Y  +N L  A  +   +   G  P   TY  LV    K + ++EA  +  ++  +G  
Sbjct: 604 KQYCIRNNLKAATAIYKDMCSRGVGPDGKTYENLVKGHCKARNMKEAWFLFQEMKGKGFS 663

Query: 627 LPIKVRISLCDMYSRAGIEKKALQALKILEAKKQE 662
           + +     L   + +    KK L+A ++ +  ++E
Sbjct: 664 VSVSTYSVLIKGFLK---RKKFLEAREVFDQMRRE 695


HSP 4 Score: 72.4 bits (176), Expect = 2.3e-11
Identity = 59/249 (23.69%), Postives = 114/249 (45.78%), Query Frame = 1

Query: 314 ISGMCKLVTEKILLLNENDDPSPLLAE-WKELLQPTRIDWITLLDRLNEKNRFLYFKVAE 373
           I G+CK          + D  + LL E WK  LQP    + ++++ L +       + A 
Sbjct: 463 IDGLCKE--------GDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNI---EEAV 522

Query: 374 LLLSE---ESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMY 433
            L+ E        +   Y+ L++ Y K   ++ A+ IL +M  KG+ P I+T   L++ +
Sbjct: 523 KLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGF 582

Query: 434 SKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQ 493
              G L+  +K  + + + G  P+   +NS++  Y      K   ++ +DM +R + P  
Sbjct: 583 CLHGMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDG 642

Query: 494 DIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDY 553
             Y  L++   +   +  A  +   M+  G S ++ + ++L++ + +     +AR  FD 
Sbjct: 643 KTYENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQ 700

Query: 554 MIKLGHAPD 559
           M + G A D
Sbjct: 703 MRREGLAAD 700

BLAST of Cucsa.232030.3 vs. Swiss-Prot
Match: PP413_ARATH (Pentatricopeptide repeat-containing protein At5g42310, mitochondrial OS=Arabidopsis thaliana GN=At5g42310 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 4.7e-25
Identity = 76/318 (23.90%), Postives = 149/318 (46.86%), Query Frame = 1

Query: 390 LIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHG 449
           +I   A   R  +AE +  ++ + GI P       L+  Y K G L  A+     +   G
Sbjct: 310 IISALADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRG 369

Query: 450 FQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAG 509
             PDE  Y+ +I AYVNAG+ +    ++++MEA D++P+  ++  LL  F   G      
Sbjct: 370 VSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTF 429

Query: 510 RIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAY 569
           ++   M+  G+ P+ +   ++++ +G+    D A   FD M+  G  PD     ++I  +
Sbjct: 430 QVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCH 489

Query: 570 EKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPI 629
            K      A ++   +E+ G  P   TY ++++  G  +  ++ +++L K+ +QG    +
Sbjct: 490 CKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNV 549

Query: 630 KVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGV 689
               +L D+Y ++G    A++ L+ +++   +     +  +IN     G  + A     V
Sbjct: 550 VTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRV 609

Query: 690 MEAQGFIASQPLQMALRT 708
           M + G    +P  +AL +
Sbjct: 610 MTSDGL---KPSLLALNS 624


HSP 2 Score: 104.4 bits (259), Expect = 5.4e-21
Identity = 77/342 (22.51%), Postives = 162/342 (47.37%), Query Frame = 1

Query: 319 KLVTEKILLLNENDDPSPLLAEWKELLQPTRID-----WITLLDRLNEKNRFLYFKVAEL 378
           +LV + I+   ++ DPS  L +   + Q T +       ++++  L +  R L  +    
Sbjct: 270 QLVNDIIMGFAKSGDPSKAL-QLLGMAQATGLSAKTATLVSIISALADSGRTLEAEALFE 329

Query: 379 LLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAG 438
            L +   +   R Y+ L++ Y K   L+DAE ++ +M ++G++PD  T   LI  Y  AG
Sbjct: 330 ELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAG 389

Query: 439 NLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYM 498
             +SA+     + +   QP+  V++ ++  + + G+ +    ++++M++  +KP +  Y 
Sbjct: 390 RWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYN 449

Query: 499 ALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKL 558
            ++ +F +   +  A      M   GI P+  +   L++ + + G    A   F+ M + 
Sbjct: 450 VVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERR 509

Query: 559 GHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEA 618
           G  P       MI +Y  +   D    LL +++  G  P + T+  LVD  GK     +A
Sbjct: 510 GCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDA 569

Query: 619 EQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKIL 656
            + L ++ + G      +  +L + Y++ G+ ++A+ A +++
Sbjct: 570 IECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVM 610


HSP 3 Score: 94.4 bits (233), Expect = 5.6e-18
Identity = 67/283 (23.67%), Postives = 122/283 (43.11%), Query Frame = 1

Query: 342 KELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLE 401
           K  + P    +  L+D      R+   ++    +     Q N   +S+L+  +      +
Sbjct: 367 KRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQ 426

Query: 402 DAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMI 461
              ++L +M   G+ PD      +I  + K   LD A   FD + S G +PD   +N++I
Sbjct: 427 KTFQVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLI 486

Query: 462 MAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGIS 521
             +   G+  + E +   ME R   P    Y  ++ S+          R+   M+  GI 
Sbjct: 487 DCHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGIL 546

Query: 522 PNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDL 581
           PN+ + T LV+ YG++G  + A    + M  +G  P      ++I AY ++ L ++A++ 
Sbjct: 547 PNVVTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNA 606

Query: 582 LLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQG 625
              +  DG +P L     L++  G+ +   EA  VL  +   G
Sbjct: 607 FRVMTSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQYMKENG 649


HSP 4 Score: 93.6 bits (231), Expect = 9.5e-18
Identity = 63/242 (26.03%), Postives = 119/242 (49.17%), Query Frame = 1

Query: 343 ELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIR--------DYSKLIEVY 402
           E ++P R+ W TL+D         + K    +++EE F+   R         Y+ +I  Y
Sbjct: 473 EGIEPDRVTWNTLID--------CHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSY 532

Query: 403 AKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDE 462
             + R +D +R+L KM  +GI P+++T   L+ +Y K+G  + A +  + ++S G +P  
Sbjct: 533 GDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSS 592

Query: 463 KVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAAT 522
            +YN++I AY   G  +   +  R M +  +KPS     +L+ +F +    + A  +   
Sbjct: 593 TMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQY 652

Query: 523 MQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAA--YEKK 575
           M+  G+ P++ + T L++A  +     K    ++ MI  G  PD +  + + +A  Y K+
Sbjct: 653 MKENGVKPDVVTYTTLMKALIRVDKFQKVPVVYEEMIMSGCKPDRKARSMLRSALRYMKQ 706


HSP 5 Score: 87.8 bits (216), Expect = 5.2e-16
Identity = 67/324 (20.68%), Postives = 146/324 (45.06%), Query Frame = 1

Query: 375 LSEESFQTNIRDYSKLIEVYAKENRLEDAE--RILVKMNEKGIAPDILTTIYLIHMYSKA 434
           + ++ +Q++  +YS +I+   + N+++     R+  ++    +  D+     +I  ++K+
Sbjct: 223 MRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKEIERDKLELDVQLVNDIIMGFAKS 282

Query: 435 GNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIY 494
           G+   A +     ++ G         S+I A  ++G+    E+L  ++    IKP    Y
Sbjct: 283 GDPSKALQLLGMAQATGLSAKTATLVSIISALADSGRTLEAEALFEELRQSGIKPRTRAY 342

Query: 495 MALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIK 554
            ALL+ + + G +  A  + + M+  G+SP+  + +LL++AY  AG  + AR     M  
Sbjct: 343 NALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEA 402

Query: 555 LGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEE 614
               P+    + ++A +  +    K   +L +++  G +P    Y V++D  GK   ++ 
Sbjct: 403 GDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDH 462

Query: 615 AEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIIN 674
           A     ++ ++G         +L D + + G    A +  + +E +        +  +IN
Sbjct: 463 AMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMIN 522

Query: 675 GLVAGGFLQDAKRMEGVMEAQGFI 697
                    D KR+ G M++QG +
Sbjct: 523 SYGDQERWDDMKRLLGKMKSQGIL 546


HSP 6 Score: 69.3 bits (168), Expect = 1.9e-10
Identity = 54/257 (21.01%), Postives = 111/257 (43.19%), Query Frame = 1

Query: 337 LLAEWKEL-LQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYA 396
           +L E K + ++P R  +  ++D   + N   +       +  E  + +   ++ LI+ + 
Sbjct: 431 VLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHC 490

Query: 397 KENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEK 456
           K  R   AE +   M  +G  P   T   +I+ Y      D  K+    ++S G  P+  
Sbjct: 491 KHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVV 550

Query: 457 VYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATM 516
            + +++  Y  +G+       + +M++  +KPS  +Y AL+ +++Q G    A      M
Sbjct: 551 THTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVM 610

Query: 517 QFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLL 576
              G+ P+L +   L+ A+G+     +A     YM + G  PD     +++ A  + +  
Sbjct: 611 TSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKF 670

Query: 577 DKALDLLLQLEKDGFEP 593
            K   +  ++   G +P
Sbjct: 671 QKVPVVYEEMIMSGCKP 687


HSP 7 Score: 36.6 bits (83), Expect = 1.4e+00
Identity = 25/125 (20.00%), Postives = 61/125 (48.80%), Query Frame = 1

Query: 564 SMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAE--QVLAKIG 623
           ++I A  + N ++KAL+L+ ++ +DG++     Y++++  L +   ++     ++  +I 
Sbjct: 202 ALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKEIE 261

Query: 624 AQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQ 683
                L +++   +   ++++G   KALQ L + +A            II+ L   G   
Sbjct: 262 RDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVSIISALADSGRTL 321

Query: 684 DAKRM 687
           +A+ +
Sbjct: 322 EAEAL 326

BLAST of Cucsa.232030.3 vs. TrEMBL
Match: A0A0A0LL29_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G361790 PE=4 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 717/717 (100.00%), Postives = 717/717 (100.00%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF
Sbjct: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60

Query: 61  ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF 120
           ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF
Sbjct: 61  ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF 120

Query: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD 180
           PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD
Sbjct: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD 180

Query: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ 240
           DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ
Sbjct: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ 240

Query: 241 LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH 300
           LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH
Sbjct: 241 LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH 300

Query: 301 VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360
           VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN
Sbjct: 301 VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360

Query: 361 EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL 420
           EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL
Sbjct: 361 EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL 420

Query: 421 TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480
           TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM
Sbjct: 421 TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480

Query: 481 EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540
           EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP
Sbjct: 481 EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540

Query: 541 DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL 600
           DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL
Sbjct: 541 DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL 600

Query: 601 VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ 660
           VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ
Sbjct: 601 VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ 660

Query: 661 ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP 718
           ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP
Sbjct: 661 ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP 717

BLAST of Cucsa.232030.3 vs. TrEMBL
Match: F6H052_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g12630 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 3.3e-267
Identity = 481/721 (66.71%), Postives = 569/721 (78.92%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           M+LKHLL RQT K  S+ NG +L   S     +    T  S S+  S  G Y S   +N 
Sbjct: 1   MNLKHLLSRQTIKGLSRFNGRILSPYSYIYKDSFAITTPISHSIYSS--GSYDS-TFQNL 60

Query: 61  ELSKLNSLFSRCIH-FTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEE 120
              KL    +R  H    TKL  +  +P+ ES +   DDG+MNEFLSRFVWIMR K+ E 
Sbjct: 61  AYPKLAPSSNRYAHSLPETKLGGSGTDPELESDD--SDDGTMNEFLSRFVWIMRGKLMEV 120

Query: 121 FPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVL 180
           +   DKQT++ MLL+IV KVVSEMEKG  EQ L A+   P  D SEDLWKTV EVSN+VL
Sbjct: 121 YTGCDKQTIDGMLLIIVRKVVSEMEKGGLEQMLSAAVAAPSQDFSEDLWKTVWEVSNLVL 180

Query: 181 DDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLE 240
           DDMKKA  KEKMKGFL S EV+EM RFAGE+GIRGDMLRE RFKWAREKMEESEFY+SL+
Sbjct: 181 DDMKKARNKEKMKGFLQSEEVKEMSRFAGEIGIRGDMLRELRFKWAREKMEESEFYQSLD 240

Query: 241 QLRKEARTQEE-----NKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSE 300
            LR+EA+ +E      N++         E K + VSLPKR GK++Y+IYGLDLSDPKW+E
Sbjct: 241 HLREEAQAEEGEEAVGNEEVIGDDLVEDEKKEKVVSLPKRHGKIRYEIYGLDLSDPKWTE 300

Query: 301 LADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWIT 360
           +ADK+H  E++I PQEPKPISG CKL+TEKIL + E DDPSPLL EW ELLQP+RIDWIT
Sbjct: 301 VADKVHEREEIIWPQEPKPISGKCKLITEKILNMKEEDDPSPLLDEWAELLQPSRIDWIT 360

Query: 361 LLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKG 420
           LLDRL E N  LYFKVAEL+LS++SFQTNIRDYSKLI+V+AKENR+EDAERIL KMNE  
Sbjct: 361 LLDRLKENNSHLYFKVAELVLSKKSFQTNIRDYSKLIDVHAKENRVEDAERILKKMNEND 420

Query: 421 IAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGE 480
           I PDILT+  L+HMYSKAGNL+ AK+AF+ LRS GFQPD +VYNSMIMAYVNAGQPKLGE
Sbjct: 421 ILPDILTSTVLVHMYSKAGNLERAKEAFEGLRSQGFQPDTRVYNSMIMAYVNAGQPKLGE 480

Query: 481 SLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAY 540
           SLMR+MEARDIKP+++IYM+LL+SF+Q G + GA RI+ TMQFAG  P+LESCTLLVEAY
Sbjct: 481 SLMREMEARDIKPTKEIYMSLLQSFAQRGDIGGAQRISTTMQFAGFQPSLESCTLLVEAY 540

Query: 541 GQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGL 600
           GQAGDPD+ARN+FDYMIK+GH PDDRCTASMIAAYEK NLLDKAL+LLLQLEKDGFEPG+
Sbjct: 541 GQAGDPDQARNSFDYMIKVGHRPDDRCTASMIAAYEKGNLLDKALNLLLQLEKDGFEPGV 600

Query: 601 ATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKI 660
           ATY VLVDWLGK+QLV+EAEQ+L KI  QG   P+K  +SLCDMYSRAG+EKKALQAL +
Sbjct: 601 ATYVVLVDWLGKMQLVDEAEQLLGKIAEQGEAPPLKFHVSLCDMYSRAGVEKKALQALGV 660

Query: 661 LEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGK 716
           +EAKK++L  +DFERII GL+AGGF+QDA+R+ GVME QGF AS+ L++ L +SQA+  +
Sbjct: 661 VEAKKEQLNPEDFERIIKGLIAGGFVQDARRIHGVMETQGFTASEQLKIILMSSQAVGRE 716

BLAST of Cucsa.232030.3 vs. TrEMBL
Match: V4TF11_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000434mg PE=4 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 9.5e-259
Identity = 469/725 (64.69%), Postives = 567/725 (78.21%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLD--RQSPNINATHIFITKPS---FSLLDSHRGYYSSL 60
           M+LKHLL RQ  KN SK+ G   +  R    I+   +  T P    F+L  + +     L
Sbjct: 1   MNLKHLLSRQITKNLSKLKGVSFNCQRNLSTISVPVLPSTSPKSHDFTLSQNPKTAKPGL 60

Query: 61  AARNFELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREK 120
           + R +  S              T+   ++I PK +S E ED D  MNEFLSRFVWIMR K
Sbjct: 61  SFRRYIFSNQE-----------TERIASSISPKTDSEEEEDRD--MNEFLSRFVWIMRGK 120

Query: 121 ISEEFPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVS 180
           +S+ +PD DK+T++ MLLMIV KVVSEMEKG  E+ +++++ +P  D SEDLW+TV EVS
Sbjct: 121 MSDAYPDSDKKTIDGMLLMIVGKVVSEMEKGGLERMIESASASPSQDFSEDLWRTVWEVS 180

Query: 181 NMVLDDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFY 240
           N+VL+DM+KA KKEKMKGFL S +V+EM RFAGE+GIRGDMLRE RFKWARE+MEESEFY
Sbjct: 181 NLVLEDMEKARKKEKMKGFLQSDKVKEMSRFAGEIGIRGDMLRELRFKWAREEMEESEFY 240

Query: 241 ESLEQLRKEARTQE--ENKDSASGAEAAS-EVKSEAVSLPKRRGKLKYKIYGLDLSDPKW 300
           E LE+LRKEA  QE  E  +  + AE A  E K + ++LPKR GK+KYKIYGLDLSDPKW
Sbjct: 241 EGLERLRKEANAQEMGEKAEGKAAAECAEVEEKPKVITLPKRHGKIKYKIYGLDLSDPKW 300

Query: 301 SELADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDW 360
           +E+A+K+H   ++ILP+EPKPI+G CKL+T+KIL L + +DPSPLLAEWKELLQP+RIDW
Sbjct: 301 AEVAEKIHERGEMILPEEPKPITGKCKLITDKILSLEKEEDPSPLLAEWKELLQPSRIDW 360

Query: 361 ITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNE 420
           I LLDRL E+N  LYFKVAEL+LSEESFQTN+RDYSKLI+ +AKEN LEDAERIL KMNE
Sbjct: 361 INLLDRLREQNTQLYFKVAELVLSEESFQTNVRDYSKLIDAHAKENCLEDAERILKKMNE 420

Query: 421 KGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKL 480
            GI PDI+T+  L+HMYSKAGNLD AK+AF+SLRSHGFQPD+KVYNSMIMAYVNAGQPKL
Sbjct: 421 NGIVPDIVTSTVLVHMYSKAGNLDRAKEAFESLRSHGFQPDKKVYNSMIMAYVNAGQPKL 480

Query: 481 GESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVE 540
           G SL+  M    I+ S++IY+ALLRSF+QCG V GAG+I   M+     P LESCTLLVE
Sbjct: 481 GMSLVDMMITSGIERSEEIYLALLRSFAQCGDVRGAGQITNIMRIEEFQPTLESCTLLVE 540

Query: 541 AYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEP 600
           AYGQAGDPD+AR+NFDYMI+LGH PDDRCTASMIAAY KKNLLDKAL+LLL+LEKDGFEP
Sbjct: 541 AYGQAGDPDQARSNFDYMIRLGHKPDDRCTASMIAAYGKKNLLDKALNLLLELEKDGFEP 600

Query: 601 GLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQAL 660
           G ATY VLVDWLG+LQL+ EAEQ+L KI   G   P K+++SLCDMY+RAGIEKKALQAL
Sbjct: 601 GPATYTVLVDWLGRLQLINEAEQLLGKISELGEAPPFKIQVSLCDMYARAGIEKKALQAL 660

Query: 661 KILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALR 718
             LEAKK+++G DDFERIINGL+AGGFLQDA+R+ G+MEAQGF AS+ L++AL +SQ   
Sbjct: 661 GFLEAKKEQMGPDDFERIINGLLAGGFLQDAQRVHGLMEAQGFAASERLKVALISSQTFN 712

BLAST of Cucsa.232030.3 vs. TrEMBL
Match: W9QQ22_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_012512 PE=4 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 1.2e-248
Identity = 453/716 (63.27%), Postives = 546/716 (76.26%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           M  KHL+  Q     SKING L  + SP I+     ++ P  +        Y S  ++N 
Sbjct: 1   MYWKHLISGQIINKISKINGRLRSK-SPYISENSFAMSAPVSTSSFHSSNTYGSKPSQNP 60

Query: 61  ELSKLNSLFSRCIHFT-VTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEE 120
           +  KLN+ F R IH T +TKLS      K ES E  D+DG+MNEFLSRFVWIMR K+SE 
Sbjct: 61  QCQKLNASFRRYIHSTRLTKLSYVEDGLKLESDE-SDEDGTMNEFLSRFVWIMRGKLSEA 120

Query: 121 FPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVL 180
             D DK+T++ MLL+IVEKVVSE+EKG  EQ L ++  +   D SEDLW TV EVSNMVL
Sbjct: 121 CGDCDKRTIDGMLLIIVEKVVSELEKGGVEQMLGSAMSSSSQDFSEDLWNTVWEVSNMVL 180

Query: 181 DDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLE 240
           DDMKKATKKEKMK  L   EV+EM RFA E+GIRGDMLRE RFKWAR+K+E+S+FYE LE
Sbjct: 181 DDMKKATKKEKMKRVLQCEEVKEMVRFASEIGIRGDMLREMRFKWARQKIEKSDFYEGLE 240

Query: 241 QLRKEARTQEENKDSASGAEAAS-EVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADK 300
           + R E + +E+ K +    E A  E + + VSLPKRRGK++YKIYGLDLS PKW+E+ADK
Sbjct: 241 RFRAEEKAREKAKATTDVEEGAVVEGRPKTVSLPKRRGKIRYKIYGLDLSGPKWTEVADK 300

Query: 301 LHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDR 360
           +H   ++I P+EPKPISG  KLVTEKIL LNE DDP+PLLAEW ELLQP+R DW   LDR
Sbjct: 301 VHEVGEMIWPEEPKPISGKSKLVTEKILALNEEDDPTPLLAEWAELLQPSRTDWTVFLDR 360

Query: 361 LNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPD 420
           L E+N  LY KVAELLLSE+SFQTN+RDYSKLI+ +AKENRL+DAERIL KM E G+ PD
Sbjct: 361 LKEENPGLYLKVAELLLSEKSFQTNVRDYSKLIDAHAKENRLKDAERILEKMKEDGVMPD 420

Query: 421 ILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMR 480
           +LT   L+HMYSKAGNLD AK+ F+SL+ HGF+PD KVYNS+IMAYVNAGQPKL ESLMR
Sbjct: 421 LLTCTVLVHMYSKAGNLDRAKETFESLKMHGFRPDLKVYNSIIMAYVNAGQPKLAESLMR 480

Query: 481 DMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAG 540
           +MEA+D+K + +IYMALLRSFSQ G VSGAGRIA TMQFAG  PN E+C LLV+A  +AG
Sbjct: 481 EMEAKDVKATNEIYMALLRSFSQSGDVSGAGRIATTMQFAGFQPNFETCKLLVQACSRAG 540

Query: 541 DPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYA 600
           +PD+AR+NFDYM+K+G  PDD C ASMIAAYEKKNLLDKAL+LLLQLEKDGF PG+ATY 
Sbjct: 541 EPDQARSNFDYMMKIGQRPDDECIASMIAAYEKKNLLDKALNLLLQLEKDGFLPGVATYI 600

Query: 601 VLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAK 660
           VL+DWLGKLQL+ EAE++L KI  QG   P ++ ISLCDMYSRAGIEKKALQAL +LEAK
Sbjct: 601 VLLDWLGKLQLINEAEELLGKISQQGEAPPFQIHISLCDMYSRAGIEKKALQALGVLEAK 660

Query: 661 KQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGK 715
           K EL  +DFERIIN L AGGF + A+RM  +MEA+GF AS+ +++  R  + +  +
Sbjct: 661 KDELSSEDFERIINALKAGGFSKHAQRMHELMEARGFTASEKIKVNQRMHELMEAR 714

BLAST of Cucsa.232030.3 vs. TrEMBL
Match: A0A061FBT4_THECC (Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_034061 PE=4 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.5e-248
Identity = 458/721 (63.52%), Postives = 551/721 (76.42%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           M+LKHL+  +T  N SKING  +      ++      + P +SL        SS   RN 
Sbjct: 1   MNLKHLISGKTINNLSKINGKSISYNPKKLSTIAAARSPPLYSLSSGQ----SSAFLRN- 60

Query: 61  ELSKLNSLFSRCIHFTV-TKLSNAAIEPKPESAEVEDD-DGSMNEFLSRFVWIMREKISE 120
              K    F R IH T  TKLS   +E K  S++ E++ DG++NEFLSRFVWIMR K+S+
Sbjct: 61  --RKPIFPFIRYIHSTQETKLSYTGVEAKLVSSDSEEEEDGTVNEFLSRFVWIMRGKLSD 120

Query: 121 EFPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMV 180
            + D +K+T++ MLL+IV KVV EMEKG  +Q L ++   P  D SEDLW+TV EVSNMV
Sbjct: 121 AYRDCNKETIDGMLLIIVGKVVEEMEKGGIQQMLDSAVAMPSQDFSEDLWRTVWEVSNMV 180

Query: 181 LDDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESL 240
           L+DM+KA KKEK+K FL S EV+EMCRFA EVGI GD+LRE RFKWA EKMEE++FY SL
Sbjct: 181 LEDMEKARKKEKVKQFLQSEEVKEMCRFASEVGIHGDLLRELRFKWAIEKMEENDFYRSL 240

Query: 241 EQLRKEARTQEENKDSASGAEAA--SEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELA 300
           E+LR E    E+ KD  +  E A  +E +   VSLPKR+GKLKYKIYGLDLSDPKW+E+A
Sbjct: 241 ERLRDE----EQPKDGKTFDEQAVVAEDEQRVVSLPKRKGKLKYKIYGLDLSDPKWAEVA 300

Query: 301 DKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLL 360
           DK+H  E ++ PQEPK +SG CKLV EKIL L + DDPS +LAEW ELLQP+R+DWI+LL
Sbjct: 301 DKIHEREAMVWPQEPKSLSGKCKLVMEKILSLKQEDDPSQILAEWIELLQPSRVDWISLL 360

Query: 361 DRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIA 420
           DRL ++N  ++ KVAEL+LSE+SFQ +I DY KLI+ YAKENR+EDAERIL  M E G+ 
Sbjct: 361 DRLEQQNPDIHLKVAELVLSEKSFQADIPDYRKLIDAYAKENRIEDAERILKMMVENGVM 420

Query: 421 PDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESL 480
           PDILTT  L+HMYSK GNLD AK+AF+SLRS+GFQPD K+YNSMIM YVNAGQPKL ESL
Sbjct: 421 PDILTTTVLVHMYSKVGNLDRAKEAFESLRSYGFQPDMKLYNSMIMGYVNAGQPKLAESL 480

Query: 481 MRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQ 540
           MR+ME RDIKPS++IYMALLRS++    V GAGRIA TMQFAG  P +ESC+LLVEAYGQ
Sbjct: 481 MREMETRDIKPSEEIYMALLRSYAHSCDVIGAGRIAITMQFAGFQPTMESCSLLVEAYGQ 540

Query: 541 AGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLAT 600
           AGD D+AR+NF+ MIKLGH PDD+CTASMIAAYEKKNLLDKALDLLLQLEKDGFEPG+AT
Sbjct: 541 AGDSDQARSNFNNMIKLGHKPDDKCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGIAT 600

Query: 601 YAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILE 660
           Y VLVDWLGKLQLV+EAEQ+L KI   G   P+KV +SLCDMYSRAG E+KALQAL +LE
Sbjct: 601 YTVLVDWLGKLQLVDEAEQLLGKIADLGEVPPLKVHVSLCDMYSRAGFERKALQALGVLE 660

Query: 661 AKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRL 718
           AKK ELG  +FER I+ L+AGGF++DA+R+ G+MEA+GF ASQ L  AL  SQ   G R 
Sbjct: 661 AKKDELGPHEFERTIHALIAGGFVKDARRIHGLMEARGFTASQQLNFALSVSQT-HGPRR 709

BLAST of Cucsa.232030.3 vs. TAIR10
Match: AT1G19520.1 (AT1G19520.1 pentatricopeptide (PPR) repeat-containing protein)

HSP 1 Score: 716.1 bits (1847), Expect = 2.2e-206
Identity = 371/694 (53.46%), Postives = 503/694 (72.48%), Query Frame = 1

Query: 21  NLLDRQSPNINATHIFITKPSFSLLDSH-RGYYSSLAARNFELSKLNSLFSRCIHFTVTK 80
           +LL  ++P        IT  + S  D H R Y      +N E+ +  S F+R  HFT   
Sbjct: 15  SLLSSKTPTFCRNFSAITS-TISHSDRHLRSYDEQTPFQNVEIPRPISSFNRYFHFTRES 74

Query: 81  L---SNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEFPDYDKQTVNAMLLMIV 140
               S+AAI+   +  E +D+DG+ NEFLSRFVWIMR K+SE +PD DK+ ++ MLL+IV
Sbjct: 75  RLSESSAAIDDSNDQEE-DDEDGTTNEFLSRFVWIMRGKVSEAYPDCDKKMIDGMLLLIV 134

Query: 141 EKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLDDMKKATKKEKMKGFLL 200
           EKVV E+E+G F + + ++  +P  + S+DLW T+ EVSN VL DM+K  KKEKMK ++ 
Sbjct: 135 EKVVEEIERGGFNK-VGSAPPSPSSEFSDDLWATIWEVSNTVLKDMEKERKKEKMKQYVQ 194

Query: 201 SREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQLR------KEARTQEE 260
           S EV EMCRFAGE+GIRGD+LRE RFKWAREKM+++EFYESLEQ R      +E+ T + 
Sbjct: 195 SPEVMEMCRFAGEIGIRGDLLRELRFKWAREKMDDAEFYESLEQQRDLDNSIRESETVDG 254

Query: 261 NKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLHVAEKLILPQE 320
             +   G   + EV+S ++SLPKR+GKLKYKIYGL+LSDPKW E+ADK+H AE+    +E
Sbjct: 255 EVEE-EGFVPSDEVESRSISLPKRKGKLKYKIYGLELSDPKWVEMADKIHEAEEEADWRE 314

Query: 321 PKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLNEKNRFLYFKV 380
           PKP++G CKLV EK+  L E DDPS LLAEW ELL+P R+DWI L+++L E N   Y KV
Sbjct: 315 PKPVTGKCKLVMEKLESLQEGDDPSGLLAEWAELLEPNRVDWIALINQLREGNTHAYLKV 374

Query: 381 AELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYS 440
           AE +L E+SF  +I DYSKLI ++AKEN +ED ERIL KM++ GI PDILT   L+HMYS
Sbjct: 375 AEGVLDEKSFNASISDYSKLIHIHAKENHIEDVERILKKMSQNGIFPDILTATALVHMYS 434

Query: 441 KAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQD 500
           K+GN + A +AF++L+S+G +PDEK+Y +MI+ YVNAG+PKLGE LM++M+A+++K S++
Sbjct: 435 KSGNFERATEAFENLKSYGLRPDEKIYEAMILGYVNAGKPKLGERLMKEMQAKELKASEE 494

Query: 501 IYMALLRSFSQCGYVSGAGRIAATMQFAGISP-NLESCTLLVEAYGQAGDPDKARNNFDY 560
           +YMALLR+++Q G  +GA  I+++MQ+A   P + E+ +L VEAYG+AG  DKA++NFD 
Sbjct: 495 VYMALLRAYAQMGDANGAAGISSSMQYASDGPLSFEAYSLFVEAYGKAGQVDKAKSNFDE 554

Query: 561 MIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQL 620
           M KLGH PDD+C A+++ AY+ +N LDKAL LLLQLEKDG E G+ TY VLVDW+  L L
Sbjct: 555 MRKLGHKPDDKCIANLVRAYKGENSLDKALRLLLQLEKDGIEIGVITYTVLVDWMANLGL 614

Query: 621 VEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFER 680
           +EEAEQ+L KI   G   P ++++SLC MYS    EKK LQAL +LEAK+ ++G ++F++
Sbjct: 615 IEEAEQLLVKISQLGEAPPFELQVSLCCMYSGVRNEKKTLQALGVLEAKRDQMGPNEFDK 674

Query: 681 IINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQM 704
           +I+ L  GGF +DA+RM   MEA+ F+ SQ LQM
Sbjct: 675 VISALKRGGFEKDARRMYKYMEARKFLPSQRLQM 704


HSP 2 Score: 55.5 bits (132), Expect = 1.6e-07
Identity = 39/139 (28.06%), Postives = 66/139 (47.48%), Query Frame = 1

Query: 373 LLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKA 432
           L L ++  +  +  Y+ L++  A    +E+AE++LVK+++ G AP     + L  MYS  
Sbjct: 584 LQLEKDGIEIGVITYTVLVDWMANLGLIEEAEQLLVKISQLGEAPPFELQVSLCCMYSGV 643

Query: 433 GNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIY 492
            N     +A   L +   Q     ++ +I A    G  K    + + MEAR   PSQ + 
Sbjct: 644 RNEKKTLQALGVLEAKRDQMGPNEFDKVISALKRGGFEKDARRMYKYMEARKFLPSQRLQ 703

Query: 493 MALLRSFSQCGYVSGAGRI 512
           M ++      G  SG+GR+
Sbjct: 704 MDMVAPPRAFG--SGSGRV 720

BLAST of Cucsa.232030.3 vs. TAIR10
Match: AT1G01970.1 (AT1G01970.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 231.1 bits (588), Expect = 2.1e-60
Identity = 124/332 (37.35%), Postives = 193/332 (58.13%), Query Frame = 1

Query: 292 WSELADKLHVAEKLILPQEPKPISGMCKLVTEKILLLN-ENDDPSPLLAEWKELLQPTRI 351
           W+++   L   +   + + P  +S  C+ +  +I+  + E      LL  W   + P R 
Sbjct: 72  WADVGLNLTEEQDEAITRIPIKMSKRCQALMRQIICFSPEKGSFCDLLGAWLRRMNPIRA 131

Query: 352 DWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKM 411
           DW+++L  L   +   Y KVAE  L ++SF+ N RDY+K+I  Y K N++EDAER L+ M
Sbjct: 132 DWLSILKELKNLDSPFYIKVAEFSLLQDSFEANARDYTKIIHYYGKLNQVEDAERTLLSM 191

Query: 412 NEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQP 471
             +G   D +T   ++ +YSKAG    A++ F+ ++  G   D + Y SMIMAY+ AG P
Sbjct: 192 KNRGFLIDQVTLTAMVQLYSKAGCHKLAEETFNEIKLLGEPLDYRSYGSMIMAYIRAGVP 251

Query: 472 KLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLL 531
           + GESL+R+M++++I   +++Y ALLR +S  G   GA R+   +Q AGI+P+++ C LL
Sbjct: 252 EKGESLLREMDSQEICAGREVYKALLRDYSMGGDAEGAKRVFDAVQIAGITPDVKLCGLL 311

Query: 532 VEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGF 591
           + AY  +G    AR  F+ M K G    D+C A ++AAYEK+  L++AL  L++LEKD  
Sbjct: 312 INAYSVSGQSQNARLAFENMRKAGIKATDKCVALVLAAYEKEEKLNEALGFLVELEKDSI 371

Query: 592 EPGLATYAVLVDWLGKLQLVEEAEQVLAKIGA 623
             G    AVL  W  KL +VEE E +L +  +
Sbjct: 372 MLGKEASAVLAQWFKKLGVVEEVELLLREFSS 403


HSP 2 Score: 48.5 bits (114), Expect = 2.0e-05
Identity = 29/129 (22.48%), Postives = 59/129 (45.74%), Query Frame = 1

Query: 585 LEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGI 644
           L +D FE     Y  ++ + GKL  VE+AE+ L  +  +G  +      ++  +YS+AG 
Sbjct: 156 LLQDSFEANARDYTKIIHYYGKLNQVEDAERTLLSMKNRGFLIDQVTLTAMVQLYSKAGC 215

Query: 645 EKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMA 704
            K A +    ++   + L +  +  +I   +  G  +  + +   M++Q   A + +  A
Sbjct: 216 HKLAEETFNEIKLLGEPLDYRSYGSMIMAYIRAGVPEKGESLLREMDSQEICAGREVYKA 275

Query: 705 LRTSQALRG 714
           L    ++ G
Sbjct: 276 LLRDYSMGG 284

BLAST of Cucsa.232030.3 vs. TAIR10
Match: AT1G05670.1 (AT1G05670.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 129.0 bits (323), Expect = 1.1e-29
Identity = 90/345 (26.09%), Postives = 163/345 (47.25%), Query Frame = 1

Query: 350 IDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVK 409
           I ++  L R+ E +  L      LL+  + +  ++  YS ++  Y +   L+   +++  
Sbjct: 253 IHFVCQLGRIKEAHHLL------LLMELKGYTPDVISYSTVVNGYCRFGELDKVWKLIEV 312

Query: 410 MNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQ 469
           M  KG+ P+      +I +  +   L  A++AF  +   G  PD  VY ++I  +   G 
Sbjct: 313 MKRKGLKPNSYIYGSIIGLLCRICKLAEAEEAFSEMIRQGILPDTVVYTTLIDGFCKRGD 372

Query: 470 PKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTL 529
            +       +M +RDI P    Y A++  F Q G +  AG++   M   G+ P+  + T 
Sbjct: 373 IRAASKFFYEMHSRDITPDVLTYTAIISGFCQIGDMVEAGKLFHEMFCKGLEPDSVTFTE 432

Query: 530 LVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDG 589
           L+  Y +AG    A    ++MI+ G +P+     ++I    K+  LD A +LL ++ K G
Sbjct: 433 LINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMWKIG 492

Query: 590 FEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKAL 649
            +P + TY  +V+ L K   +EEA +++ +  A G         +L D Y ++G   KA 
Sbjct: 493 LQPNIFTYNSIVNGLCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQ 552

Query: 650 QALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQG 695
           + LK +  K  +     F  ++NG    G L+D +++   M A+G
Sbjct: 553 EILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKG 591


HSP 2 Score: 92.8 bits (229), Expect = 9.1e-19
Identity = 57/242 (23.55%), Postives = 116/242 (47.93%), Query Frame = 1

Query: 383 NIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAF 442
           N+  Y+ LI+   KE  L+ A  +L +M + G+ P+I T   +++   K+GN++ A K  
Sbjct: 455 NVVTYTTLIDGLCKEGDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNIEEAVKLV 514

Query: 443 DSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQC 502
               + G   D   Y +++ AY  +G+    + ++++M  + ++P+   +  L+  F   
Sbjct: 515 GEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGFCLH 574

Query: 503 GYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCT 562
           G +    ++   M   GI+PN  +   LV+ Y    +   A   +  M   G  PD +  
Sbjct: 575 GMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDGKTY 634

Query: 563 ASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGA 622
            +++  + K   + +A  L  +++  GF   ++TY+VL+    K +   EA +V  ++  
Sbjct: 635 ENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQMRR 694

Query: 623 QG 625
           +G
Sbjct: 695 EG 696


HSP 3 Score: 84.7 bits (208), Expect = 2.5e-16
Identity = 64/275 (23.27%), Postives = 124/275 (45.09%), Query Frame = 1

Query: 387 YSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLR 446
           +++LI  Y K   ++DA R+   M + G +P+++T   LI    K G+LDSA +    + 
Sbjct: 424 FTELINGYCKAGHMKDAFRVHNHMIQAGCSPNVVTYTTLIDGLCKEGDLDSANELLHEMW 483

Query: 447 SHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVS 506
             G QP+   YNS++     +G  +    L+ + EA  +      Y  L+ ++ + G + 
Sbjct: 484 KIGLQPNIFTYNSIVNGLCKSGNIEEAVKLVGEFEAAGLNADTVTYTTLMDAYCKSGEMD 543

Query: 507 GAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMI 566
            A  I   M   G+ P + +  +L+  +   G  +      ++M+  G AP+     S++
Sbjct: 544 KAQEILKEMLGKGLQPTIVTFNVLMNGFCLHGMLEDGEKLLNWMLAKGIAPNATTFNSLV 603

Query: 567 AAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHY 626
             Y  +N L  A  +   +   G  P   TY  LV    K + ++EA  +  ++  +G  
Sbjct: 604 KQYCIRNNLKAATAIYKDMCSRGVGPDGKTYENLVKGHCKARNMKEAWFLFQEMKGKGFS 663

Query: 627 LPIKVRISLCDMYSRAGIEKKALQALKILEAKKQE 662
           + +     L   + +    KK L+A ++ +  ++E
Sbjct: 664 VSVSTYSVLIKGFLK---RKKFLEAREVFDQMRRE 695


HSP 4 Score: 72.4 bits (176), Expect = 1.3e-12
Identity = 59/249 (23.69%), Postives = 114/249 (45.78%), Query Frame = 1

Query: 314 ISGMCKLVTEKILLLNENDDPSPLLAE-WKELLQPTRIDWITLLDRLNEKNRFLYFKVAE 373
           I G+CK          + D  + LL E WK  LQP    + ++++ L +       + A 
Sbjct: 463 IDGLCKE--------GDLDSANELLHEMWKIGLQPNIFTYNSIVNGLCKSGNI---EEAV 522

Query: 374 LLLSE---ESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMY 433
            L+ E        +   Y+ L++ Y K   ++ A+ IL +M  KG+ P I+T   L++ +
Sbjct: 523 KLVGEFEAAGLNADTVTYTTLMDAYCKSGEMDKAQEILKEMLGKGLQPTIVTFNVLMNGF 582

Query: 434 SKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQ 493
              G L+  +K  + + + G  P+   +NS++  Y      K   ++ +DM +R + P  
Sbjct: 583 CLHGMLEDGEKLLNWMLAKGIAPNATTFNSLVKQYCIRNNLKAATAIYKDMCSRGVGPDG 642

Query: 494 DIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDY 553
             Y  L++   +   +  A  +   M+  G S ++ + ++L++ + +     +AR  FD 
Sbjct: 643 KTYENLVKGHCKARNMKEAWFLFQEMKGKGFSVSVSTYSVLIKGFLKRKKFLEAREVFDQ 700

Query: 554 MIKLGHAPD 559
           M + G A D
Sbjct: 703 MRREGLAAD 700

BLAST of Cucsa.232030.3 vs. TAIR10
Match: AT5G42310.1 (AT5G42310.1 Pentatricopeptide repeat (PPR-like) superfamily protein)

HSP 1 Score: 117.9 bits (294), Expect = 2.6e-26
Identity = 76/318 (23.90%), Postives = 149/318 (46.86%), Query Frame = 1

Query: 390 LIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHG 449
           +I   A   R  +AE +  ++ + GI P       L+  Y K G L  A+     +   G
Sbjct: 310 IISALADSGRTLEAEALFEELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRG 369

Query: 450 FQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAG 509
             PDE  Y+ +I AYVNAG+ +    ++++MEA D++P+  ++  LL  F   G      
Sbjct: 370 VSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTF 429

Query: 510 RIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAY 569
           ++   M+  G+ P+ +   ++++ +G+    D A   FD M+  G  PD     ++I  +
Sbjct: 430 QVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCH 489

Query: 570 EKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPI 629
            K      A ++   +E+ G  P   TY ++++  G  +  ++ +++L K+ +QG    +
Sbjct: 490 CKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNV 549

Query: 630 KVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGV 689
               +L D+Y ++G    A++ L+ +++   +     +  +IN     G  + A     V
Sbjct: 550 VTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRV 609

Query: 690 MEAQGFIASQPLQMALRT 708
           M + G    +P  +AL +
Sbjct: 610 MTSDGL---KPSLLALNS 624


HSP 2 Score: 104.4 bits (259), Expect = 3.0e-22
Identity = 77/342 (22.51%), Postives = 162/342 (47.37%), Query Frame = 1

Query: 319 KLVTEKILLLNENDDPSPLLAEWKELLQPTRID-----WITLLDRLNEKNRFLYFKVAEL 378
           +LV + I+   ++ DPS  L +   + Q T +       ++++  L +  R L  +    
Sbjct: 270 QLVNDIIMGFAKSGDPSKAL-QLLGMAQATGLSAKTATLVSIISALADSGRTLEAEALFE 329

Query: 379 LLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAG 438
            L +   +   R Y+ L++ Y K   L+DAE ++ +M ++G++PD  T   LI  Y  AG
Sbjct: 330 ELRQSGIKPRTRAYNALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAG 389

Query: 439 NLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYM 498
             +SA+     + +   QP+  V++ ++  + + G+ +    ++++M++  +KP +  Y 
Sbjct: 390 RWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYN 449

Query: 499 ALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKL 558
            ++ +F +   +  A      M   GI P+  +   L++ + + G    A   F+ M + 
Sbjct: 450 VVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERR 509

Query: 559 GHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEA 618
           G  P       MI +Y  +   D    LL +++  G  P + T+  LVD  GK     +A
Sbjct: 510 GCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDA 569

Query: 619 EQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKIL 656
            + L ++ + G      +  +L + Y++ G+ ++A+ A +++
Sbjct: 570 IECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVM 610


HSP 3 Score: 94.4 bits (233), Expect = 3.1e-19
Identity = 67/283 (23.67%), Postives = 122/283 (43.11%), Query Frame = 1

Query: 342 KELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLE 401
           K  + P    +  L+D      R+   ++    +     Q N   +S+L+  +      +
Sbjct: 367 KRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEAGDVQPNSFVFSRLLAGFRDRGEWQ 426

Query: 402 DAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMI 461
              ++L +M   G+ PD      +I  + K   LD A   FD + S G +PD   +N++I
Sbjct: 427 KTFQVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLI 486

Query: 462 MAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGIS 521
             +   G+  + E +   ME R   P    Y  ++ S+          R+   M+  GI 
Sbjct: 487 DCHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGIL 546

Query: 522 PNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDL 581
           PN+ + T LV+ YG++G  + A    + M  +G  P      ++I AY ++ L ++A++ 
Sbjct: 547 PNVVTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNA 606

Query: 582 LLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLAKIGAQG 625
              +  DG +P L     L++  G+ +   EA  VL  +   G
Sbjct: 607 FRVMTSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQYMKENG 649


HSP 4 Score: 93.6 bits (231), Expect = 5.4e-19
Identity = 63/242 (26.03%), Postives = 119/242 (49.17%), Query Frame = 1

Query: 343 ELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIR--------DYSKLIEVY 402
           E ++P R+ W TL+D         + K    +++EE F+   R         Y+ +I  Y
Sbjct: 473 EGIEPDRVTWNTLID--------CHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMINSY 532

Query: 403 AKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDE 462
             + R +D +R+L KM  +GI P+++T   L+ +Y K+G  + A +  + ++S G +P  
Sbjct: 533 GDQERWDDMKRLLGKMKSQGILPNVVTHTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSS 592

Query: 463 KVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAAT 522
            +YN++I AY   G  +   +  R M +  +KPS     +L+ +F +    + A  +   
Sbjct: 593 TMYNALINAYAQRGLSEQAVNAFRVMTSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQY 652

Query: 523 MQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAA--YEKK 575
           M+  G+ P++ + T L++A  +     K    ++ MI  G  PD +  + + +A  Y K+
Sbjct: 653 MKENGVKPDVVTYTTLMKALIRVDKFQKVPVVYEEMIMSGCKPDRKARSMLRSALRYMKQ 706


HSP 5 Score: 87.8 bits (216), Expect = 2.9e-17
Identity = 67/324 (20.68%), Postives = 146/324 (45.06%), Query Frame = 1

Query: 375 LSEESFQTNIRDYSKLIEVYAKENRLEDAE--RILVKMNEKGIAPDILTTIYLIHMYSKA 434
           + ++ +Q++  +YS +I+   + N+++     R+  ++    +  D+     +I  ++K+
Sbjct: 223 MRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKEIERDKLELDVQLVNDIIMGFAKS 282

Query: 435 GNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIY 494
           G+   A +     ++ G         S+I A  ++G+    E+L  ++    IKP    Y
Sbjct: 283 GDPSKALQLLGMAQATGLSAKTATLVSIISALADSGRTLEAEALFEELRQSGIKPRTRAY 342

Query: 495 MALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIK 554
            ALL+ + + G +  A  + + M+  G+SP+  + +LL++AY  AG  + AR     M  
Sbjct: 343 NALLKGYVKTGPLKDAESMVSEMEKRGVSPDEHTYSLLIDAYVNAGRWESARIVLKEMEA 402

Query: 555 LGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEE 614
               P+    + ++A +  +    K   +L +++  G +P    Y V++D  GK   ++ 
Sbjct: 403 GDVQPNSFVFSRLLAGFRDRGEWQKTFQVLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDH 462

Query: 615 AEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIIN 674
           A     ++ ++G         +L D + + G    A +  + +E +        +  +IN
Sbjct: 463 AMTTFDRMLSEGIEPDRVTWNTLIDCHCKHGRHIVAEEMFEAMERRGCLPCATTYNIMIN 522

Query: 675 GLVAGGFLQDAKRMEGVMEAQGFI 697
                    D KR+ G M++QG +
Sbjct: 523 SYGDQERWDDMKRLLGKMKSQGIL 546


HSP 6 Score: 69.3 bits (168), Expect = 1.1e-11
Identity = 54/257 (21.01%), Postives = 111/257 (43.19%), Query Frame = 1

Query: 337 LLAEWKEL-LQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYA 396
           +L E K + ++P R  +  ++D   + N   +       +  E  + +   ++ LI+ + 
Sbjct: 431 VLKEMKSIGVKPDRQFYNVVIDTFGKFNCLDHAMTTFDRMLSEGIEPDRVTWNTLIDCHC 490

Query: 397 KENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEK 456
           K  R   AE +   M  +G  P   T   +I+ Y      D  K+    ++S G  P+  
Sbjct: 491 KHGRHIVAEEMFEAMERRGCLPCATTYNIMINSYGDQERWDDMKRLLGKMKSQGILPNVV 550

Query: 457 VYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATM 516
            + +++  Y  +G+       + +M++  +KPS  +Y AL+ +++Q G    A      M
Sbjct: 551 THTTLVDVYGKSGRFNDAIECLEEMKSVGLKPSSTMYNALINAYAQRGLSEQAVNAFRVM 610

Query: 517 QFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLL 576
              G+ P+L +   L+ A+G+     +A     YM + G  PD     +++ A  + +  
Sbjct: 611 TSDGLKPSLLALNSLINAFGEDRRDAEAFAVLQYMKENGVKPDVVTYTTLMKALIRVDKF 670

Query: 577 DKALDLLLQLEKDGFEP 593
            K   +  ++   G +P
Sbjct: 671 QKVPVVYEEMIMSGCKP 687


HSP 7 Score: 36.6 bits (83), Expect = 7.8e-02
Identity = 25/125 (20.00%), Postives = 61/125 (48.80%), Query Frame = 1

Query: 564 SMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAE--QVLAKIG 623
           ++I A  + N ++KAL+L+ ++ +DG++     Y++++  L +   ++     ++  +I 
Sbjct: 202 ALIGACARNNDIEKALNLIAKMRQDGYQSDFVNYSLVIQSLTRSNKIDSVMLLRLYKEIE 261

Query: 624 AQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGGFLQ 683
                L +++   +   ++++G   KALQ L + +A            II+ L   G   
Sbjct: 262 RDKLELDVQLVNDIIMGFAKSGDPSKALQLLGMAQATGLSAKTATLVSIISALADSGRTL 321

Query: 684 DAKRM 687
           +A+ +
Sbjct: 322 EAEAL 326

BLAST of Cucsa.232030.3 vs. TAIR10
Match: AT2G35130.2 (AT2G35130.2 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 112.1 bits (279), Expect = 1.5e-24
Identity = 92/377 (24.40%), Postives = 167/377 (44.30%), Query Frame = 1

Query: 326 LLLNENDDPSPLLAEW---KELLQPTRIDWITLLDRLNEKNRFLYFKVAELLLSEESFQT 385
           L LN+  D   L+ EW   K   QP  I +  L+D   +K ++   +   + L E  +  
Sbjct: 151 LRLNKKWDSIILVCEWILRKSSFQPDVICFNLLIDAYGQKFQYKEAESLYVQLLESRYVP 210

Query: 386 NIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL-TTIYLIH---MYSKAGNLDSA 445
               Y+ LI+ Y     +E AE +LV+M    ++P  +  T+Y  +   +  + GN + A
Sbjct: 211 TEDTYALLIKAYCMAGLIERAEVVLVEMQNHHVSPKTIGVTVYNAYIEGLMKRKGNTEEA 270

Query: 446 KKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRS 505
              F  ++    +P  + YN MI  Y  A +  +   L  +M +   KP+   Y AL+ +
Sbjct: 271 IDVFQRMKRDRCKPTTETYNLMINLYGKASKSYMSWKLYCEMRSHQCKPNICTYTALVNA 330

Query: 506 FSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLGHAPD 565
           F++ G    A  I   +Q  G+ P++     L+E+Y +AG P  A   F  M  +G  PD
Sbjct: 331 FAREGLCEKAEEIFEQLQEDGLEPDVYVYNALMESYSRAGYPYGAAEIFSLMQHMGCEPD 390

Query: 566 DRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQVLA 625
                 M+ AY +  L   A  +  ++++ G  P + ++ +L+    K + V + E ++ 
Sbjct: 391 RASYNIMVDAYGRAGLHSDAEAVFEEMKRLGIAPTMKSHMLLLSAYSKARDVTKCEAIVK 450

Query: 626 KIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQELGHDDFERIINGLVAGG 685
           ++   G      V  S+ ++Y R G   K  + L  +E          +  +IN     G
Sbjct: 451 EMSENGVEPDTFVLNSMLNLYGRLGQFTKMEKILAEMENGPCTADISTYNILINIYGKAG 510

Query: 686 FLQDAKRMEGVMEAQGF 696
           FL+  + +   ++ + F
Sbjct: 511 FLERIEELFVELKEKNF 527


HSP 2 Score: 95.1 bits (235), Expect = 1.8e-19
Identity = 65/245 (26.53%), Postives = 113/245 (46.12%), Query Frame = 1

Query: 383 NIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAF 442
           NI  Y+ L+  +A+E   E AE I  ++ E G+ PD+     L+  YS+AG    A + F
Sbjct: 320 NICTYTALVNAFAREGLCEKAEEIFEQLQEDGLEPDVYVYNALMESYSRAGYPYGAAEIF 379

Query: 443 DSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMALLRSFSQC 502
             ++  G +PD   YN M+ AY  AG     E++  +M+   I P+   +M LL ++S+ 
Sbjct: 380 SLMQHMGCEPDRASYNIMVDAYGRAGLHSDAEAVFEEMKRLGIAPTMKSHMLLLSAYSKA 439

Query: 503 GYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIK-LGHAPDDRC 562
             V+    I   M   G+ P+      ++  YG+ G        F  M K L    +  C
Sbjct: 440 RDVTKCEAIVKEMSENGVEPDTFVLNSMLNLYGRLG-------QFTKMEKILAEMENGPC 499

Query: 563 TAS------MIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAEQ 621
           TA       +I  Y K   L++  +L ++L++  F P + T+   +    + +L  +  +
Sbjct: 500 TADISTYNILINIYGKAGFLERIEELFVELKEKNFRPDVVTWTSRIGAYSRKKLYVKCLE 557


HSP 3 Score: 87.8 bits (216), Expect = 2.9e-17
Identity = 56/243 (23.05%), Postives = 109/243 (44.86%), Query Frame = 1

Query: 375 LSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDILTTIYLIHMYSKAGN 434
           L E+  + ++  Y+ L+E Y++      A  I   M   G  PD  +   ++  Y +AG 
Sbjct: 347 LQEDGLEPDVYVYNALMESYSRAGYPYGAAEIFSLMQHMGCEPDRASYNIMVDAYGRAGL 406

Query: 435 LDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDMEARDIKPSQDIYMA 494
              A+  F+ ++  G  P  K +  ++ AY  A      E+++++M    ++P   +  +
Sbjct: 407 HSDAEAVFEEMKRLGIAPTMKSHMLLLSAYSKARDVTKCEAIVKEMSENGVEPDTFVLNS 466

Query: 495 LLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDPDKARNNFDYMIKLG 554
           +L  + + G  +   +I A M+    + ++ +  +L+  YG+AG  ++    F  + +  
Sbjct: 467 MLNLYGRLGQFTKMEKILAEMENGPCTADISTYNILINIYGKAGFLERIEELFVELKEKN 526

Query: 555 HAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVLVDWLGKLQLVEEAE 614
             PD     S I AY +K L  K L++  ++   G  P   T  VL+      + VE+  
Sbjct: 527 FRPDVVTWTSRIGAYSRKKLYVKCLEVFEEMIDSGCAPDGGTAKVLLSACSSEEQVEQVT 586

Query: 615 QVL 618
            VL
Sbjct: 587 SVL 589


HSP 4 Score: 47.0 bits (110), Expect = 5.7e-05
Identity = 36/133 (27.07%), Postives = 56/133 (42.11%), Query Frame = 1

Query: 351 DWITLLDRLNEKNRFLYFKVAELLLSEES---FQTNIRDYSKLIEVYAKENRLEDAERIL 410
           D   L   LN   R   F   E +L+E        +I  Y+ LI +Y K   LE  E + 
Sbjct: 460 DTFVLNSMLNLYGRLGQFTKMEKILAEMENGPCTADISTYNILINIYGKAGFLERIEELF 519

Query: 411 VKMNEKGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNA 470
           V++ EK   PD++T    I  YS+        + F+ +   G  PD      ++ A  + 
Sbjct: 520 VELKEKNFRPDVVTWTSRIGAYSRKKLYVKCLEVFEEMIDSGCAPDGGTAKVLLSACSSE 579

Query: 471 GQPKLGESLMRDM 481
            Q +   S++R M
Sbjct: 580 EQVEQVTSVLRTM 592

BLAST of Cucsa.232030.3 vs. NCBI nr
Match: gi|449470136|ref|XP_004152774.1| (PREDICTED: uncharacterized protein LOC101215563 [Cucumis sativus])

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 717/717 (100.00%), Postives = 717/717 (100.00%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF
Sbjct: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60

Query: 61  ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF 120
           ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF
Sbjct: 61  ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF 120

Query: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD 180
           PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD
Sbjct: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD 180

Query: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ 240
           DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ
Sbjct: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ 240

Query: 241 LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH 300
           LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH
Sbjct: 241 LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH 300

Query: 301 VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360
           VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN
Sbjct: 301 VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360

Query: 361 EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL 420
           EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL
Sbjct: 361 EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL 420

Query: 421 TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480
           TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM
Sbjct: 421 TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480

Query: 481 EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540
           EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP
Sbjct: 481 EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540

Query: 541 DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL 600
           DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL
Sbjct: 541 DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL 600

Query: 601 VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ 660
           VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ
Sbjct: 601 VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ 660

Query: 661 ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP 718
           ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP
Sbjct: 661 ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP 717

BLAST of Cucsa.232030.3 vs. NCBI nr
Match: gi|659087970|ref|XP_008444732.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At5g59900 [Cucumis melo])

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 657/717 (91.63%), Postives = 685/717 (95.54%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           MSLKHLLLRQTRKNFSKINGNLLDRQSP+INATHIFITKPSFSLLDSH GYYSS+AARNF
Sbjct: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPSINATHIFITKPSFSLLDSHHGYYSSIAARNF 60

Query: 61  ELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEEF 120
           ELSK NS+FSRCIHFTVTKL+NAAIE KPESAEVEDDDGSMNEFLSRFVWIMR KISE F
Sbjct: 61  ELSKSNSIFSRCIHFTVTKLNNAAIELKPESAEVEDDDGSMNEFLSRFVWIMRGKISEAF 120

Query: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVLD 180
           PDYDKQTVNAMLLMIVEKVVSEMEKG FEQTLK+STDNPDWDLSEDLWKTV+EVSNMVLD
Sbjct: 121 PDYDKQTVNAMLLMIVEKVVSEMEKGSFEQTLKSSTDNPDWDLSEDLWKTVSEVSNMVLD 180

Query: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLEQ 240
           DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYE LEQ
Sbjct: 181 DMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYEGLEQ 240

Query: 241 LRKEARTQEENKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSELADKLH 300
           LRK+A TQEE  DSASG EAASEVKSEA SLPKRRGKLKYKIYGLDLSD KWSE+ADK+H
Sbjct: 241 LRKKAHTQEETYDSASGTEAASEVKSEAFSLPKRRGKLKYKIYGLDLSDTKWSEVADKIH 300

Query: 301 VAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360
            A +++ P EPKPISGMCKLVTE+IL LNENDDPSPLLAEWKELLQPTRIDWITLLDRLN
Sbjct: 301 EAGQMLWPPEPKPISGMCKLVTERILSLNENDDPSPLLAEWKELLQPTRIDWITLLDRLN 360

Query: 361 EKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKGIAPDIL 420
           EKNRFLYFKVAELLL+EESFQTNIRDYSKL++VYAKE+RLEDAERIL+KMNEKGI PDIL
Sbjct: 361 EKNRFLYFKVAELLLNEESFQTNIRDYSKLVDVYAKESRLEDAERILMKMNEKGITPDIL 420

Query: 421 TTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480
           T   L+HMYSK GNLD AK+AFD+L+SHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM
Sbjct: 421 TATVLVHMYSKVGNLDRAKEAFDTLKSHGFQPDEKVYNSMIMAYVNAGQPKLGESLMRDM 480

Query: 481 EARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540
           EARDIKPSQDIYMALLRSFSQCG VSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP
Sbjct: 481 EARDIKPSQDIYMALLRSFSQCGNVSGAGRIAATMQFAGISPNLESCTLLVEAYGQAGDP 540

Query: 541 DKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLATYAVL 600
           D+ARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGL TYAVL
Sbjct: 541 DQARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGLLTYAVL 600

Query: 601 VDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKILEAKKQ 660
           VDWLGKLQLV+EAEQVL KIGA+GH  PIKVRISLCDMYSRAGIEKKALQAL+ILEAKK+
Sbjct: 601 VDWLGKLQLVDEAEQVLGKIGARGHSFPIKVRISLCDMYSRAGIEKKALQALRILEAKKE 660

Query: 661 ELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGKRLP 718
           ELGH DFERIINGLVAGGFLQDAKRM GVMEAQGF ASQPLQMALRTSQALRG+RLP
Sbjct: 661 ELGHADFERIINGLVAGGFLQDAKRMAGVMEAQGFTASQPLQMALRTSQALRGRRLP 717

BLAST of Cucsa.232030.3 vs. NCBI nr
Match: gi|302142507|emb|CBI19710.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 929.1 bits (2400), Expect = 4.7e-267
Identity = 481/721 (66.71%), Postives = 569/721 (78.92%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           M+LKHLL RQT K  S+ NG +L   S     +    T  S S+  S  G Y S   +N 
Sbjct: 1   MNLKHLLSRQTIKGLSRFNGRILSPYSYIYKDSFAITTPISHSIYSS--GSYDS-TFQNL 60

Query: 61  ELSKLNSLFSRCIH-FTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEE 120
              KL    +R  H    TKL  +  +P+ ES +   DDG+MNEFLSRFVWIMR K+ E 
Sbjct: 61  AYPKLAPSSNRYAHSLPETKLGGSGTDPELESDD--SDDGTMNEFLSRFVWIMRGKLMEV 120

Query: 121 FPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVL 180
           +   DKQT++ MLL+IV KVVSEMEKG  EQ L A+   P  D SEDLWKTV EVSN+VL
Sbjct: 121 YTGCDKQTIDGMLLIIVRKVVSEMEKGGLEQMLSAAVAAPSQDFSEDLWKTVWEVSNLVL 180

Query: 181 DDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLE 240
           DDMKKA  KEKMKGFL S EV+EM RFAGE+GIRGDMLRE RFKWAREKMEESEFY+SL+
Sbjct: 181 DDMKKARNKEKMKGFLQSEEVKEMSRFAGEIGIRGDMLRELRFKWAREKMEESEFYQSLD 240

Query: 241 QLRKEARTQEE-----NKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSE 300
            LR+EA+ +E      N++         E K + VSLPKR GK++Y+IYGLDLSDPKW+E
Sbjct: 241 HLREEAQAEEGEEAVGNEEVIGDDLVEDEKKEKVVSLPKRHGKIRYEIYGLDLSDPKWTE 300

Query: 301 LADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWIT 360
           +ADK+H  E++I PQEPKPISG CKL+TEKIL + E DDPSPLL EW ELLQP+RIDWIT
Sbjct: 301 VADKVHEREEIIWPQEPKPISGKCKLITEKILNMKEEDDPSPLLDEWAELLQPSRIDWIT 360

Query: 361 LLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKG 420
           LLDRL E N  LYFKVAEL+LS++SFQTNIRDYSKLI+V+AKENR+EDAERIL KMNE  
Sbjct: 361 LLDRLKENNSHLYFKVAELVLSKKSFQTNIRDYSKLIDVHAKENRVEDAERILKKMNEND 420

Query: 421 IAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGE 480
           I PDILT+  L+HMYSKAGNL+ AK+AF+ LRS GFQPD +VYNSMIMAYVNAGQPKLGE
Sbjct: 421 ILPDILTSTVLVHMYSKAGNLERAKEAFEGLRSQGFQPDTRVYNSMIMAYVNAGQPKLGE 480

Query: 481 SLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAY 540
           SLMR+MEARDIKP+++IYM+LL+SF+Q G + GA RI+ TMQFAG  P+LESCTLLVEAY
Sbjct: 481 SLMREMEARDIKPTKEIYMSLLQSFAQRGDIGGAQRISTTMQFAGFQPSLESCTLLVEAY 540

Query: 541 GQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGL 600
           GQAGDPD+ARN+FDYMIK+GH PDDRCTASMIAAYEK NLLDKAL+LLLQLEKDGFEPG+
Sbjct: 541 GQAGDPDQARNSFDYMIKVGHRPDDRCTASMIAAYEKGNLLDKALNLLLQLEKDGFEPGV 600

Query: 601 ATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKI 660
           ATY VLVDWLGK+QLV+EAEQ+L KI  QG   P+K  +SLCDMYSRAG+EKKALQAL +
Sbjct: 601 ATYVVLVDWLGKMQLVDEAEQLLGKIAEQGEAPPLKFHVSLCDMYSRAGVEKKALQALGV 660

Query: 661 LEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGK 716
           +EAKK++L  +DFERII GL+AGGF+QDA+R+ GVME QGF AS+ L++ L +SQA+  +
Sbjct: 661 VEAKKEQLNPEDFERIIKGLIAGGFVQDARRIHGVMETQGFTASEQLKIILMSSQAVGRE 716

BLAST of Cucsa.232030.3 vs. NCBI nr
Match: gi|225458257|ref|XP_002281331.1| (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial [Vitis vinifera])

HSP 1 Score: 929.1 bits (2400), Expect = 4.7e-267
Identity = 481/721 (66.71%), Postives = 569/721 (78.92%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLDRQSPNINATHIFITKPSFSLLDSHRGYYSSLAARNF 60
           M+LKHLL RQT K  S+ NG +L   S     +    T  S S+  S  G Y S   +N 
Sbjct: 1   MNLKHLLSRQTIKGLSRFNGRILSPYSYIYKDSFAITTPISHSIYSS--GSYDS-TFQNL 60

Query: 61  ELSKLNSLFSRCIH-FTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREKISEE 120
              KL    +R  H    TKL  +  +P+ ES +   DDG+MNEFLSRFVWIMR K+ E 
Sbjct: 61  AYPKLAPSSNRYAHSLPETKLGGSGTDPELESDD--SDDGTMNEFLSRFVWIMRGKLMEV 120

Query: 121 FPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVSNMVL 180
           +   DKQT++ MLL+IV KVVSEMEKG  EQ L A+   P  D SEDLWKTV EVSN+VL
Sbjct: 121 YTGCDKQTIDGMLLIIVRKVVSEMEKGGLEQMLSAAVAAPSQDFSEDLWKTVWEVSNLVL 180

Query: 181 DDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFYESLE 240
           DDMKKA  KEKMKGFL S EV+EM RFAGE+GIRGDMLRE RFKWAREKMEESEFY+SL+
Sbjct: 181 DDMKKARNKEKMKGFLQSEEVKEMSRFAGEIGIRGDMLRELRFKWAREKMEESEFYQSLD 240

Query: 241 QLRKEARTQEE-----NKDSASGAEAASEVKSEAVSLPKRRGKLKYKIYGLDLSDPKWSE 300
            LR+EA+ +E      N++         E K + VSLPKR GK++Y+IYGLDLSDPKW+E
Sbjct: 241 HLREEAQAEEGEEAVGNEEVIGDDLVEDEKKEKVVSLPKRHGKIRYEIYGLDLSDPKWTE 300

Query: 301 LADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDWIT 360
           +ADK+H  E++I PQEPKPISG CKL+TEKIL + E DDPSPLL EW ELLQP+RIDWIT
Sbjct: 301 VADKVHEREEIIWPQEPKPISGKCKLITEKILNMKEEDDPSPLLDEWAELLQPSRIDWIT 360

Query: 361 LLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNEKG 420
           LLDRL E N  LYFKVAEL+LS++SFQTNIRDYSKLI+V+AKENR+EDAERIL KMNE  
Sbjct: 361 LLDRLKENNSHLYFKVAELVLSKKSFQTNIRDYSKLIDVHAKENRVEDAERILKKMNEND 420

Query: 421 IAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKLGE 480
           I PDILT+  L+HMYSKAGNL+ AK+AF+ LRS GFQPD +VYNSMIMAYVNAGQPKLGE
Sbjct: 421 ILPDILTSTVLVHMYSKAGNLERAKEAFEGLRSQGFQPDTRVYNSMIMAYVNAGQPKLGE 480

Query: 481 SLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVEAY 540
           SLMR+MEARDIKP+++IYM+LL+SF+Q G + GA RI+ TMQFAG  P+LESCTLLVEAY
Sbjct: 481 SLMREMEARDIKPTKEIYMSLLQSFAQRGDIGGAQRISTTMQFAGFQPSLESCTLLVEAY 540

Query: 541 GQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEPGL 600
           GQAGDPD+ARN+FDYMIK+GH PDDRCTASMIAAYEK NLLDKAL+LLLQLEKDGFEPG+
Sbjct: 541 GQAGDPDQARNSFDYMIKVGHRPDDRCTASMIAAYEKGNLLDKALNLLLQLEKDGFEPGV 600

Query: 601 ATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQALKI 660
           ATY VLVDWLGK+QLV+EAEQ+L KI  QG   P+K  +SLCDMYSRAG+EKKALQAL +
Sbjct: 601 ATYVVLVDWLGKMQLVDEAEQLLGKIAEQGEAPPLKFHVSLCDMYSRAGVEKKALQALGV 660

Query: 661 LEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALRGK 716
           +EAKK++L  +DFERII GL+AGGF+QDA+R+ GVME QGF AS+ L++ L +SQA+  +
Sbjct: 661 VEAKKEQLNPEDFERIIKGLIAGGFVQDARRIHGVMETQGFTASEQLKIILMSSQAVGRE 716

BLAST of Cucsa.232030.3 vs. NCBI nr
Match: gi|567884905|ref|XP_006435011.1| (hypothetical protein CICLE_v10000434mg [Citrus clementina])

HSP 1 Score: 901.0 bits (2327), Expect = 1.4e-258
Identity = 469/725 (64.69%), Postives = 567/725 (78.21%), Query Frame = 1

Query: 1   MSLKHLLLRQTRKNFSKINGNLLD--RQSPNINATHIFITKPS---FSLLDSHRGYYSSL 60
           M+LKHLL RQ  KN SK+ G   +  R    I+   +  T P    F+L  + +     L
Sbjct: 1   MNLKHLLSRQITKNLSKLKGVSFNCQRNLSTISVPVLPSTSPKSHDFTLSQNPKTAKPGL 60

Query: 61  AARNFELSKLNSLFSRCIHFTVTKLSNAAIEPKPESAEVEDDDGSMNEFLSRFVWIMREK 120
           + R +  S              T+   ++I PK +S E ED D  MNEFLSRFVWIMR K
Sbjct: 61  SFRRYIFSNQE-----------TERIASSISPKTDSEEEEDRD--MNEFLSRFVWIMRGK 120

Query: 121 ISEEFPDYDKQTVNAMLLMIVEKVVSEMEKGRFEQTLKASTDNPDWDLSEDLWKTVTEVS 180
           +S+ +PD DK+T++ MLLMIV KVVSEMEKG  E+ +++++ +P  D SEDLW+TV EVS
Sbjct: 121 MSDAYPDSDKKTIDGMLLMIVGKVVSEMEKGGLERMIESASASPSQDFSEDLWRTVWEVS 180

Query: 181 NMVLDDMKKATKKEKMKGFLLSREVQEMCRFAGEVGIRGDMLREFRFKWAREKMEESEFY 240
           N+VL+DM+KA KKEKMKGFL S +V+EM RFAGE+GIRGDMLRE RFKWARE+MEESEFY
Sbjct: 181 NLVLEDMEKARKKEKMKGFLQSDKVKEMSRFAGEIGIRGDMLRELRFKWAREEMEESEFY 240

Query: 241 ESLEQLRKEARTQE--ENKDSASGAEAAS-EVKSEAVSLPKRRGKLKYKIYGLDLSDPKW 300
           E LE+LRKEA  QE  E  +  + AE A  E K + ++LPKR GK+KYKIYGLDLSDPKW
Sbjct: 241 EGLERLRKEANAQEMGEKAEGKAAAECAEVEEKPKVITLPKRHGKIKYKIYGLDLSDPKW 300

Query: 301 SELADKLHVAEKLILPQEPKPISGMCKLVTEKILLLNENDDPSPLLAEWKELLQPTRIDW 360
           +E+A+K+H   ++ILP+EPKPI+G CKL+T+KIL L + +DPSPLLAEWKELLQP+RIDW
Sbjct: 301 AEVAEKIHERGEMILPEEPKPITGKCKLITDKILSLEKEEDPSPLLAEWKELLQPSRIDW 360

Query: 361 ITLLDRLNEKNRFLYFKVAELLLSEESFQTNIRDYSKLIEVYAKENRLEDAERILVKMNE 420
           I LLDRL E+N  LYFKVAEL+LSEESFQTN+RDYSKLI+ +AKEN LEDAERIL KMNE
Sbjct: 361 INLLDRLREQNTQLYFKVAELVLSEESFQTNVRDYSKLIDAHAKENCLEDAERILKKMNE 420

Query: 421 KGIAPDILTTIYLIHMYSKAGNLDSAKKAFDSLRSHGFQPDEKVYNSMIMAYVNAGQPKL 480
            GI PDI+T+  L+HMYSKAGNLD AK+AF+SLRSHGFQPD+KVYNSMIMAYVNAGQPKL
Sbjct: 421 NGIVPDIVTSTVLVHMYSKAGNLDRAKEAFESLRSHGFQPDKKVYNSMIMAYVNAGQPKL 480

Query: 481 GESLMRDMEARDIKPSQDIYMALLRSFSQCGYVSGAGRIAATMQFAGISPNLESCTLLVE 540
           G SL+  M    I+ S++IY+ALLRSF+QCG V GAG+I   M+     P LESCTLLVE
Sbjct: 481 GMSLVDMMITSGIERSEEIYLALLRSFAQCGDVRGAGQITNIMRIEEFQPTLESCTLLVE 540

Query: 541 AYGQAGDPDKARNNFDYMIKLGHAPDDRCTASMIAAYEKKNLLDKALDLLLQLEKDGFEP 600
           AYGQAGDPD+AR+NFDYMI+LGH PDDRCTASMIAAY KKNLLDKAL+LLL+LEKDGFEP
Sbjct: 541 AYGQAGDPDQARSNFDYMIRLGHKPDDRCTASMIAAYGKKNLLDKALNLLLELEKDGFEP 600

Query: 601 GLATYAVLVDWLGKLQLVEEAEQVLAKIGAQGHYLPIKVRISLCDMYSRAGIEKKALQAL 660
           G ATY VLVDWLG+LQL+ EAEQ+L KI   G   P K+++SLCDMY+RAGIEKKALQAL
Sbjct: 601 GPATYTVLVDWLGRLQLINEAEQLLGKISELGEAPPFKIQVSLCDMYARAGIEKKALQAL 660

Query: 661 KILEAKKQELGHDDFERIINGLVAGGFLQDAKRMEGVMEAQGFIASQPLQMALRTSQALR 718
             LEAKK+++G DDFERIINGL+AGGFLQDA+R+ G+MEAQGF AS+ L++AL +SQ   
Sbjct: 661 GFLEAKKEQMGPDDFERIINGLLAGGFLQDAQRVHGLMEAQGFAASERLKVALISSQTFN 712

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NFD5_ARATH1.8e-9351.94Protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial OS=Arabidopsis thaliana GN=NFD... [more]
PPR51_ARATH7.9e-8954.24Pentatricopeptide repeat-containing protein At1g19525 OS=Arabidopsis thaliana GN... [more]
PPR1_ARATH3.8e-5937.35Pentatricopeptide repeat-containing protein At1g01970 OS=Arabidopsis thaliana GN... [more]
PPR12_ARATH2.0e-2826.09Pentatricopeptide repeat-containing protein At1g05670, mitochondrial OS=Arabidop... [more]
PP413_ARATH4.7e-2523.90Pentatricopeptide repeat-containing protein At5g42310, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A0A0LL29_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G361790 PE=4 SV=1[more]
F6H052_VITVI3.3e-26766.71Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g12630 PE=4 SV=... [more]
V4TF11_9ROSI9.5e-25964.69Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000434mg PE=4 SV=1[more]
W9QQ22_9ROSA1.2e-24863.27Uncharacterized protein OS=Morus notabilis GN=L484_012512 PE=4 SV=1[more]
A0A061FBT4_THECC1.5e-24863.52Pentatricopeptide repeat-containing protein OS=Theobroma cacao GN=TCM_034061 PE=... [more]
Match NameE-valueIdentityDescription
AT1G19520.12.2e-20653.46 pentatricopeptide (PPR) repeat-containing protein[more]
AT1G01970.12.1e-6037.35 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT1G05670.11.1e-2926.09 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT5G42310.12.6e-2623.90 Pentatricopeptide repeat (PPR-like) superfamily protein[more]
AT2G35130.21.5e-2424.40 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449470136|ref|XP_004152774.1|0.0e+00100.00PREDICTED: uncharacterized protein LOC101215563 [Cucumis sativus][more]
gi|659087970|ref|XP_008444732.1|0.0e+0091.63PREDICTED: putative pentatricopeptide repeat-containing protein At5g59900 [Cucum... [more]
gi|302142507|emb|CBI19710.3|4.7e-26766.71unnamed protein product [Vitis vinifera][more]
gi|225458257|ref|XP_002281331.1|4.7e-26766.71PREDICTED: protein NUCLEAR FUSION DEFECTIVE 5, mitochondrial [Vitis vinifera][more]
gi|567884905|ref|XP_006435011.1|1.4e-25864.69hypothetical protein CICLE_v10000434mg [Citrus clementina][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.232030Cucsa.232030gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.232030.3Cucsa.232030.3-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.232030.3.five_prime_UTR.1Cucsa.232030.3.five_prime_UTR.1five_prime_UTR
Cucsa.232030.3.five_prime_UTR.2Cucsa.232030.3.five_prime_UTR.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.232030.3.CDS.1Cucsa.232030.3.CDS.1CDS
Cucsa.232030.3.CDS.2Cucsa.232030.3.CDS.2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.232030.3.three_prime_UTR.1Cucsa.232030.3.three_prime_UTR.1three_prime_UTR
Cucsa.232030.3.three_prime_UTR.2Cucsa.232030.3.three_prime_UTR.2three_prime_UTR
Cucsa.232030.3.three_prime_UTR.3Cucsa.232030.3.three_prime_UTR.3three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 564..590
score: 0.019coord: 526..554
score: 0
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 443..499
score: 8.7E-5coord: 378..430
score: 4.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 387..419
score: 1.6E-5coord: 526..558
score: 3.9E-4coord: 425..454
score: 3.8E-5coord: 456..488
score: 2.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 523..557
score: 10.337coord: 488..522
score: 7.815coord: 593..627
score: 7.125coord: 453..487
score: 11.17coord: 558..592
score: 9.065coord: 418..452
score: 10.095coord: 383..417
score: 11.246coord: 663..697
score: 5
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 383..653
score: 9.
NoneNo IPR availableunknownCoilCoilcoord: 393..413
score: -coord: 231..258
scor
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 328..696
score: 8.5E
NoneNo IPR availablePANTHERPTHR24015:SF515SUBFAMILY NOT NAMEDcoord: 328..696
score: 8.5E