Cucsa.181840.4 (mRNA) Cucumber (Gy14) v1

NameCucsa.181840.4
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionArginine/serine-rich splicing factor, putative
Locationscaffold01251 : 81403 .. 86255 (-)
Sequence length1477
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAAGAATCATCTCTTCTGTTTTTTTCTTCTTTCTTGCCCTTATAATAATCAGGTACCCATTTCTCTGTTTTGAATTTTCATTTCTTTTTGGAATTTTTGTTCTTTGATTCTTGATAGAAGAAAGAAGAAAATTAGAAAAGAAGAAAGAAGAAGAAGATCTCGTCATCTTTGTATGTTGATTTTCTTTTTCATTACAATTTTGTCTTAATTTTGATAGCCATGGTGATGATTGATGACTATGATCATGGCTCAATAGGTTATTCTTCTTCATCCCTTTTTAAATCTGTATATTGTTCTTTTTCTATCTTTGTGATGCGATTTTTGTAACATTTGTTCTTATCTTTTTCAAGATTTTGTATTTGTTGTTCACATCAAAGGAGAATATGAGCAGCAGAAACAGTCGTACCATCTATGTTGGGAATCTTCCTGGTGACATTCGTCTGAGAGAAGTAGAAGATCTGTTCTACAAGGTTTGAAATTTTAGTTCTTGATAATTTTAAACTTCTTGTTGTTGTCTTGAGATGAGTAATTCTAGTATAATTGCAGTATGGACCCATTGTTGATATTGATTTGAAGATTCCACCACGACCACCGGGCTATGCTTTTGTTGAGGTGAGATTTAAATTTTGGAATATTTGGAACCCTGTTTCTAATTCTACGACCCTCAAATTGTGTTTTAGAGTTTTGGGTATTCAAAGAAGCTAAGTAGTTGCACTGAGCTGTTAGTGGTCCTTATGGAAAAACTCTTTGCTTTTCAGTTTGAAGATGTTCGTGATGCTGAAGATGCCATTTATGGCCGAGATGGGTATAAGTTTGATGGATGTCGATTGCGGGTACTAGTTGCTACGCGGGCTTGCTGATATTACTTGCCTGTGATGTCTTGTCCACATATCTGAATATAATTTTGAAATGCTTGCTTTGTAGGTGGAATTTGCACATGGTGGAAGGGGACATTCTTCTTCTGTGGATCGTTACAGTCGTAGTGGAAGTAGTCGTGGTGGTGTTCCTCGCCGAACTGACTATCGGGGTGTGTATTTTATCACTTTTTTATTTTGTGAAAGTCCGATTATAGTTTTGTGATGATATTTTGTTGTGCAGTTTTGGTTACTGGATTACCTTCTTCTGCTTCATGGCAAGACCTGAAGGTAAGCACGCTATTTCTGTTTCTGAAAATGGCATGCTACTGTAGTAAGTTGTCTATTTCTTTTCTCGATCATAGCATCTTTGTCGATTAGAGAGAACGGATTAGAGAGAACGAATTAGTGTATGGCTCCACCCTTTCTATTAATTGAGAGTTTGCTATGTGAATTTGATAATATTCAGGATCACATGCGTCGTGCTGGTGATGTCTGCTTCTCTGAGGTTTTCCGTGATCGTGGTGGTATGCCTTATTAACTTATTTCTCTTTCTATTTGCTCTTACATCTGTCTCTGTGCAAGTGTCATTTAAACACATGTTTTTTTTTTCAATATATTTGAAATGTATGTGCATGTTTGGACTTTGGACAGCTTCATTGCAGATTGATATTAGTATTGCAGTATTAACCTCCGTATTATTTTTTTCTATGAAAAAAATATCCTTGTTTTTAGATTACCTTCTTGCCACTATTTAGATTACCTTCTTGCCACATTCTATTTCATAAAGAATGTGAATTAATGAACACAGTAGGCTGGTTGAAAAGGTTCTTTAAGACATGTTAGAGATGAGGTTGATAGGTCTGAAATCCCCAACTTGAACAAGTACCTGGGGTAAGACAAATAGATTCTTCATGTCTTGGTCAACAAGACCTTTCATAGAGCTCTCTGAATACATAACTCTAGTCCCAAATCTCCATGGTGAAGTCCAAATTGGCCATTGCACCAAGTTGTTTATGCCCTGTCATGTTTTTTCTTTATTTATTTTTTAGTTCTACCTTGTGGTTGATATTGTATACATTTGAGTGTTTTCAGTCTCACGCTTGTTTCAGTTTTGTGTATATTTTCTTCATTTCTTGTATTCTATAAATCATGCTTAGTGGTGCTGAAAACCATTTTTTAAAGAATAATTTTTATGTAAATCTGTGCAGGGATGGCAGGAATAGTTGATTATACAAACTATGATGACATGAAATATGCTGTAAGTTGCACTTTAGATGGCTCGACTCTCGATGTTTTTCTGGAAGATTTGGTTCATGTTTAATATTGACAGTCTCTGACTCTGTTTTGGACTTTGTGTTCCTGCGATAATTTATTTTAATTATGTTTATAATTTTTTATCTGGCTGCAGATTAGAAAACTTGATGATTCTGAATTTCGGAATGCCTTTTCTCGAGCATATGTACGGGTACGTTGTCCGGAGTATGTGGACTGGAAGTTGTTTATATATTCTACTAAATTCTATTTTGTTGTCATTATTTTTTCAAATAAAAATGTGTACCTATTCACTCAGGTGAAGGAATATGACTCTAGACACAGCTACTCCAGGAGTCCATCTCTTGATTCTAGACGAAGTGATTATTCTAGAAGTCCTAGTCGTAGTCCATACAGGGGAAGAGGCCGGAGTCAGAGCCGAAGCAGAAGTCGAAGCCGTAGCCGTAGCCGTAGTTATAGCGGCAGGAGCACTAGGTATGTTTTAGTATTCTTGCAAAATAAAGACTTATATGTCGAATCATGAAGACTAGTATGATGTGACAATTTGTTTTGCAGTCTATCTCCAAGGCACAAGCACTCTCGTCGATCACGATCGGTTTCTGAACAATCCCTATCAAGGTTTGCTTTCATCCCACACACACATCATTCAATTTTCCATTCCAAGAACAGTGTCTGAATTTTCGTTGCTGTTGTTTTGGTGCTATTCATTTTAAACGACGTCGATTTTTTAACCGTCCCTATCAAAGTTTGCACACATTGATCAAGATGTGGGGAGAACGTGATTTTTGTATCTCTTCAATATGAATATTGTGTTGAATGCTCACTAATGTATTACATTGTGCACACCGAGTTCCTTTTCTTTTATGCGTTCACATCTTTTCTCCATGTTAAGAAAAAAGATACAAGGGACTTTTTGACTACCTTATCTGTTTCTTCTTGAGACCACACAATATCTATTTCTTGGCTTTTCATCTTTTGAAATGTTCTGACATTCAGATTTTGGAAATGAGAAGTGTTATGAAGTGTATCTATGTAAGTGTGCGTACTTGGTTTTTCTTTCTTCTTTCTTTGAACATAAAATGAGAATAGTTGCTGTATGCTTTTTGTTCTCTCTTTTTATGTAAAAGCAGGAAAGAAATAAGAACTACTAAAAGGCAACTGATTCAGCTCTAAAGCTTTTCTAGACATACATGGTTTAGTTGAATCTGCTGGTAGACATTGGCTTTTATGCAAAGAATGTTGAATTTTGTTTTGGTTTTCAAGCATTCTAAAAGGACAAACGTATTAACCTTCTTGAGAATCCCTTAGCGAGACTTCAACCATGCACTTGTATAATTGTCATGATTCACTTAGCAATTGTTTTCTTATGATGTCGCTCCAATAAACAAACACTGTTCAACTTCACTCCGGAAGTGGTGGAAGAAAGCATAAAAAACAAACAAGAAAGCATAAAAAACAAACAAGTATTTTTTTTTCCTTTGGATATGCTCGGGCCTCCAGTTAACAAAATAGAGGTTTGTCAGTTTGGGAAGTCATCATTGTTTGTTTATGTTTTGCTAGATATGGGCTTTTGCGGAATAGTTGGGACTTCATGTTAGGGACTTGAGGGATGTGGTGCTCAATGTGCTTCTTTCAACTCTTTGGTTGTTGAGGTAATACAGTTTCGTTGATGAGCACCCTGTCAACCTCGACGCTGATATTCTTTTCCCTTTTTCATGATTTCCCAATTGTTGCTAACGCTTGTGGAAAAGCCTGTTTAGTAGTACTCTTGTTCGGCCAGTTGATAATTACTTATCTAAAACTTTCAGACACATCAATTCTACGTTACTTTGAATGTTCTTCGTCTTTGCTCTCACCCTGGTCTGCTACTGACTGTTTATTCGCTTGAACTCTATTCTTCTATTCACCTGTTTGATATGACTGAAGACTGAGTTGAGATTAGAAACTGGTCTTCGATGTGGTGTTATTGTTGAATGTGGATTTTATCATTTTGACTTCAGCTGCAGACCCAGACTTTGCTTATGGGTACTTTAAGTCCCTTGTTTCCTGTTTTCAGGTCTAGGTCAAGGTCACGATCGAGATCAAGATCACGGTCACGATCACCAGTTTCATCTGTGAGTAGAAAATTGAATATATTATCCCCCCCTCCCCTTCCTCTGGTTTTCCTTCTCTCATGTTTCTCATTGTCTCATTGATTGCAGCGTCATCGTGCAAGCAGACCTCGAAGTCGGAGCAGATCTCGGTCCAAGTCTAGATCCCTTTCACCGAATGTAATATCCTGATAGACTTTGCATGTTCCTCAAAACATGAATAACGTATGGCTTCTGTTAGTTTCTACGAGGAAGTAATATTGACCGAGTTTTGACATAAGCAGGTGAGATCCGATCGAAGCCGAAGCCAATCTGTTGATTCCAGGGACCAATATTCAGAATAGGACATTGCTGTGAGGCTGCTCTCAGATGGAACAGTAAAAGCGTAGGAGAAAAGTAGGGAATGGTATATTTGGAGGGTTCACTTTTAATGTAAAATGTGTTATGGATGACCTTTATTAACCCAAGCCATGTTGCTGCATTGTATGTCGACACATGCTTCGGTTATTTCACTTTTAACGTTCTCTGTTCTTCCACCTTCGAGTATATTTCGTTACTATTACATATTCTCTGATGACTAGTTTTTGTTTCATGTATTTTTCTTTAAAGCAGTTATCAAGTCTTACATTATC

mRNA sequence

TTCAAGAATCATCTCTTCTGTTTTTTTCTTCTTTCTTGCCCTTATAATAATCAGCCATGGTGATGATTGATGACTATGATCATGGCTCAATAGGTTATTCTTCTTCATCCCTTTTTAAATCTGTATATTGTTCTTTTTCTATCTTTGTGATGCGATTTTTGTAACATTTGTTCTTATCTTTTTCAAGATTTTGTATTTGTTGTTCACATCAAAGGAGAATATGAGCAGCAGAAACAGTCGTACCATCTATGTTGGGAATCTTCCTGGTGACATTCGTCTGAGAGAAGTAGAAGATCTGTTCTACAAGTATGGACCCATTGTTGATATTGATTTGAAGATTCCACCACGACCACCGGGCTATGCTTTTGTTGAGTTTGAAGATGTTCGTGATGCTGAAGATGCCATTTATGGCCGAGATGGGTATAAGTTTGATGGATGTCGATTGCGGGTGGAATTTGCACATGGTGGAAGGGGACATTCTTCTTCTGTGGATCGTTACAGTCGTAGTGGAAGTAGTCGTGGTGGTGTTCCTCGCCGAACTGACTATCGGGTTTTGGTTACTGGATTACCTTCTTCTGCTTCATGGCAAGACCTGAAGGATCACATGCGTCGTGCTGGTGATGTCTGCTTCTCTGAGGTTTTCCGTGATCGTGGTGGGATGGCAGGAATAGTTGATTATACAAACTATGATGACATGAAATATGCTATTAGAAAACTTGATGATTCTGAATTTCGGAATGCCTTTTCTCGAGCATATGTACGGGTGAAGGAATATGACTCTAGACACAGCTACTCCAGGAGTCCATCTCTTGATTCTAGACGAAGTGATTATTCTAGAAGTCCTAGTCGTAGTCCATACAGGGGAAGAGGCCGGAGTCAGAGCCGAAGCAGAAGTCGAAGCCGTAGCCGTAGCCGTAGTTATAGCGGCAGGAGCACTAGTCTATCTCCAAGGCACAAGCACTCTCGTCGATCACGATCGGTTTCTGAACAATCCCTATCAAGGTCTAGGTCAAGGTCACGATCGAGATCAAGATCACGGTCACGATCACCAGTTTCATCTCGTCATCGTGCAAGCAGACCTCGAAGTCGGAGCAGATCTCGGTCCAAGTCTAGATCCCTTTCACCGAATGTGAGATCCGATCGAAGCCGAAGCCAATCTGTTGATTCCAGGGACCAATATTCAGAATAGGACATTGCTGTGAGGCTGCTCTCAGATGGAACAGTAAAAGCGTAGGAGAAAAGTAGGGAATGGTATATTTGGAGGGTTCACTTTTAATGTAAAATGTGTTATGGATGACCTTTATTAACCCAAGCCATGTTGCTGCATTGTATGTCGACACATGCTTCGGTTATTTCACTTTTAACGTTCTCTGTTCTTCCACCTTCGAGTATATTTCGTTACTATTACATATTCTCTGATGACTAGTTTTTGTTTCATGTATTTTTCTTTAAAGCAGTTATCAAGTCTTACATTATC

Coding sequence (CDS)

ATGAGCAGCAGAAACAGTCGTACCATCTATGTTGGGAATCTTCCTGGTGACATTCGTCTGAGAGAAGTAGAAGATCTGTTCTACAAGTATGGACCCATTGTTGATATTGATTTGAAGATTCCACCACGACCACCGGGCTATGCTTTTGTTGAGTTTGAAGATGTTCGTGATGCTGAAGATGCCATTTATGGCCGAGATGGGTATAAGTTTGATGGATGTCGATTGCGGGTGGAATTTGCACATGGTGGAAGGGGACATTCTTCTTCTGTGGATCGTTACAGTCGTAGTGGAAGTAGTCGTGGTGGTGTTCCTCGCCGAACTGACTATCGGGTTTTGGTTACTGGATTACCTTCTTCTGCTTCATGGCAAGACCTGAAGGATCACATGCGTCGTGCTGGTGATGTCTGCTTCTCTGAGGTTTTCCGTGATCGTGGTGGGATGGCAGGAATAGTTGATTATACAAACTATGATGACATGAAATATGCTATTAGAAAACTTGATGATTCTGAATTTCGGAATGCCTTTTCTCGAGCATATGTACGGGTGAAGGAATATGACTCTAGACACAGCTACTCCAGGAGTCCATCTCTTGATTCTAGACGAAGTGATTATTCTAGAAGTCCTAGTCGTAGTCCATACAGGGGAAGAGGCCGGAGTCAGAGCCGAAGCAGAAGTCGAAGCCGTAGCCGTAGCCGTAGTTATAGCGGCAGGAGCACTAGTCTATCTCCAAGGCACAAGCACTCTCGTCGATCACGATCGGTTTCTGAACAATCCCTATCAAGGTCTAGGTCAAGGTCACGATCGAGATCAAGATCACGGTCACGATCACCAGTTTCATCTCGTCATCGTGCAAGCAGACCTCGAAGTCGGAGCAGATCTCGGTCCAAGTCTAGATCCCTTTCACCGAATGTGAGATCCGATCGAAGCCGAAGCCAATCTGTTGATTCCAGGGACCAATATTCAGAATAG

Protein sequence

MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAEDAIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSLSPNVRSDRSRSQSVDSRDQYSE*
BLAST of Cucsa.181840.4 vs. Swiss-Prot
Match: SR30_ARATH (Serine/arginine-rich splicing factor SR30 OS=Arabidopsis thaliana GN=SR30 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.0e-101
Identity = 201/284 (70.77%), Postives = 220/284 (77.46%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR +RTIYVGNLPGDIR  EVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRWNRTIYVGNLPGDIRKCEVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDPRDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY FDGCRLRVE AHGGR  S SVDRYS S S+     RR+DYRVLVTGLP SA
Sbjct: 61  AIYGRDGYDFDGCRLRVEIAHGGRRFSPSVDRYSSSYSASRAPSRRSDYRVLVTGLPPSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMR+AGDVCFSEVF DR GM+G+VDY+NYDDMKYAIRKLD +EFRNAFS AY+
Sbjct: 121 SWQDLKDHMRKAGDVCFSEVFPDRKGMSGVVDYSNYDDMKYAIRKLDATEFRNAFSSAYI 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RV+EY+SR S SRSP       D S+S              RSRSRSR  S SYS +S S
Sbjct: 181 RVREYESR-SVSRSP-------DDSKS-------------YRSRSRSRGPSCSYSSKSRS 240

Query: 241 LSPRHKHSRRSRSVSE-----QSLSRSRSRSRSRSRSRSRSPVS 280
           +SP    S RSR +S       S+SRS+SRS+SRSRSRS SPVS
Sbjct: 241 VSPARSISPRSRPLSRSRSLYSSVSRSQSRSKSRSRSRSNSPVS 263


HSP 2 Score: 197.6 bits (501), Expect = 2.1e-49
Identity = 141/314 (44.90%), Postives = 162/314 (51.59%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR +RTIYVGNLPGDIR  EVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRWNRTIYVGNLPGDIRKCEVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDPRDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY FDGCRLRVE AHGGR  S SVDRYS                         +
Sbjct: 61  AIYGRDGYDFDGCRLRVEIAHGGRRFSPSVDRYS-------------------------S 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           S+   +   RR+           R  + G+    ++ D+K  +RK  D      FS  + 
Sbjct: 121 SYSASRAPSRRSDY---------RVLVTGLPPSASWQDLKDHMRKAGDV----CFSEVFP 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
             K       YS    +         +  R+ +           S +  R R Y  RS S
Sbjct: 181 DRKGMSGVVDYSNYDDMKYAIRKLDATEFRNAF-----------SSAYIRVREYESRSVS 240

Query: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRS-RSRSRSRSPVSSRHRASRPRSRSRSRSKSRS 300
            SP    S RSRS       RSR  S S  S+SRS SP  S    SRP SRSRS   S S
Sbjct: 241 RSPDDSKSYRSRS-------RSRGPSCSYSSKSRSVSPARSISPRSRPLSRSRSLYSSVS 258

Query: 301 LSPNVRSDRSRSQS 314
            S +    RSRS+S
Sbjct: 301 RSQSRSKSRSRSRS 258

BLAST of Cucsa.181840.4 vs. Swiss-Prot
Match: SR34_ARATH (Serine/arginine-rich-splicing factor SR34 OS=Arabidopsis thaliana GN=SR34 PE=1 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.9e-96
Identity = 197/314 (62.74%), Postives = 226/314 (71.97%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRT+YVGNLPGDIR REVEDLF KYGP+V IDLK+PPRPPGYAFVEF+D RDAED
Sbjct: 1   MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGG-----------VPRRTDY 120
           AI+GRDGY FDG RLRVE AHGGR  SS   R S +G  RGG             RR+++
Sbjct: 61  AIHGRDGYDFDGHRLRVELAHGGR-RSSDDTRGSFNGGGRGGGRGRGDGGSRGPSRRSEF 120

Query: 121 RVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDS 180
           RVLVTGLPSSASWQDLKDHMR+ GDVCFS+V+RD  G  G+VDYT Y+DMKYA++KLDD+
Sbjct: 121 RVLVTGLPSSASWQDLKDHMRKGGDVCFSQVYRDARGTTGVVDYTCYEDMKYALKKLDDT 180

Query: 181 EFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRS 240
           EFRNAFS  YVRV+EYDSR   SRSP   SR   YS+S SRS    RGRS SRSRSRSRS
Sbjct: 181 EFRNAFSNGYVRVREYDSRKD-SRSP---SRGRSYSKSRSRS----RGRSVSRSRSRSRS 240

Query: 241 RSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRS 300
           RSRS   +S+  SP       SRS   +S SRS S  RSRSRSRS  P   +  +  P  
Sbjct: 241 RSRSPKAKSSRRSPA---KSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSK 300

Query: 301 RSRSRSKSRSLSPN 304
            S ++S   + SP+
Sbjct: 301 PSPAKSPIHTRSPS 302

BLAST of Cucsa.181840.4 vs. Swiss-Prot
Match: SR34A_ARATH (Serine/arginine-rich splicing factor SR34A OS=Arabidopsis thaliana GN=SR34A PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 6.9e-93
Identity = 194/327 (59.33%), Postives = 224/327 (68.50%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MS R SR+IYVGNLPGDIR  E+ED+FYKYG IVDI+LK+PPRPP Y FVEFE  RDAED
Sbjct: 1   MSGRFSRSIYVGNLPGDIREHEIEDIFYKYGRIVDIELKVPPRPPCYCFVEFEHSRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSG-------------SSRGGVPRRT 120
           AI GRDGY  DGCRLRVE AHGGRG SSS  R    G             S+R GV R +
Sbjct: 61  AIKGRDGYNLDGCRLRVELAHGGRGQSSSDRRGGYGGGGSGYGGGGGGGGSARFGVSRHS 120

Query: 121 DYRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLD 180
           ++RV+V GLPSSASWQDLKDHMR+AGDVCF+EV RD  G  G+VDYTNYDDMKYAIRKLD
Sbjct: 121 EFRVIVRGLPSSASWQDLKDHMRKAGDVCFAEVTRDSDGTYGVVDYTNYDDMKYAIRKLD 180

Query: 181 DSEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRS 240
           D+EFRN ++R ++RVK+Y+S  S SRSPS        SRS SRS  RGRGRS SRSRS S
Sbjct: 181 DTEFRNPWARGFIRVKKYESSRSRSRSPS-----RSRSRSRSRSRSRGRGRSHSRSRSLS 240

Query: 241 RSRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRP 300
           RS+           SPR   S+  R    +SLSRS S+SRS S  + +SP        R 
Sbjct: 241 RSK-----------SPRKDLSKSPR----RSLSRSISKSRSPSPDKKKSP-------PRA 300

Query: 301 RSRSRSRSKSRSLSPNVRSDRSRSQSV 315
            SRS+SRS+SRS SP+    + R  SV
Sbjct: 301 MSRSKSRSRSRSRSPSKSPPKVREGSV 300


HSP 2 Score: 39.3 bits (90), Expect = 9.6e-02
Identity = 27/60 (45.00%), Postives = 32/60 (53.33%), Query Frame = 1

Query: 263 RSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSLSPNVRSDRSRSQSVDSRDQYSE 322
           R +    SRSRSRSP       SR RSRSRSRS+SR    +    RS S+S   R   S+
Sbjct: 194 RVKKYESSRSRSRSP-------SRSRSRSRSRSRSRGRGRSHSRSRSLSRSKSPRKDLSK 246

BLAST of Cucsa.181840.4 vs. Swiss-Prot
Match: SR34B_ARATH (Serine/arginine-rich splicing factor SR34B OS=Arabidopsis thaliana GN=SR34B PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.7e-89
Identity = 190/294 (64.63%), Postives = 209/294 (71.09%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR REVEDLF KYGP+V IDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPRPPGYAFVEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRG------------GVPRRTD 120
           AIYGRDGY FDG  LRVE AHGGR  SS   R S SG  RG            G  RR++
Sbjct: 61  AIYGRDGYDFDGHHLRVELAHGGR-RSSHDARGSYSGRGRGGRGGGDGGGRERGPSRRSE 120

Query: 121 YRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDD 180
           YRV+V+GLPSSASWQDLKDHMR+ G+VCFS+VFRD  G  GIVDYT+Y+DMKYA   LDD
Sbjct: 121 YRVVVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRGTTGIVDYTSYEDMKYA---LDD 180

Query: 181 SEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSR 240
           +EFRNAFS  YVRV+EYDSR   SRSP   SR   YS+S      R RGRS SRSRSRSR
Sbjct: 181 TEFRNAFSHEYVRVREYDSRRD-SRSP---SRGRSYSKS------RSRGRSPSRSRSRSR 240

Query: 241 SRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRH 283
           SRS+       S SP+ K  RRS + S     RSRSRS+SRS S        RH
Sbjct: 241 SRSK-------SRSPKAKSLRRSPAKSTSRSPRSRSRSKSRSLSPRGWVTVERH 273


HSP 2 Score: 164.5 bits (415), Expect = 2.0e-39
Identity = 129/319 (40.44%), Postives = 155/319 (48.59%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR REVEDLF KYGP+V IDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPRPPGYAFVEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY F              GH   V+     G  R     R  Y     G     
Sbjct: 61  AIYGRDGYDF-------------DGHHLRVEL--AHGGRRSSHDARGSYSGRGRGGRGGG 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAF--SRA 180
                    R  G    SE    R  ++G+    ++ D+K  +RK  +  F   F   R 
Sbjct: 121 DGGG-----RERGPSRRSEY---RVVVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRG 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
              + +Y S      +      R+ +S       Y       SR  SRS SR RSYS   
Sbjct: 181 TTGIVDYTSYEDMKYALDDTEFRNAFSHE-----YVRVREYDSRRDSRSPSRGRSYS--- 240

Query: 241 TSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSR 300
                                 +SRSR RS SRSRSRS   S+ R+ + +S  RS +KS 
Sbjct: 241 ----------------------KSRSRGRSPSRSRSRSRSRSKSRSPKAKSLRRSPAKST 265

Query: 301 SLSPNVRSDRSRSQSVDSR 318
           S SP  RS RS+S+S+  R
Sbjct: 301 SRSPRSRS-RSKSRSLSPR 265

BLAST of Cucsa.181840.4 vs. Swiss-Prot
Match: SRS1B_DANRE (Serine/arginine-rich splicing factor 1B OS=Danio rerio GN=srsf1b PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.1e-61
Identity = 140/259 (54.05%), Postives = 170/259 (65.64%), Query Frame = 1

Query: 2   SSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAEDA 61
           +  N   IYVGNLP DIR ++VED+FYKYG I DIDLK     P +AFVEFED RDAEDA
Sbjct: 10  AGNNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDA 69

Query: 62  IYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRG--GVP-RRTDYRVLVTGLPS 121
           +YGRDGY +DG RLRVEF   GRG           G+ RG  G P RR++YRV+V+GLP 
Sbjct: 70  VYGRDGYDYDGYRLRVEFPRSGRGGGRGGGGGGGVGAPRGRYGPPSRRSEYRVIVSGLPP 129

Query: 122 SASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFR-NAFSR 181
           S SWQDLKDHMR AGDVC+++VFRD     G+V++   +DM YA+RKLD+++FR +    
Sbjct: 130 SGSWQDLKDHMREAGDVCYADVFRDG---TGVVEFVRKEDMTYAVRKLDNTKFRSHEGET 189

Query: 182 AYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGR 241
           AY+RVK                   D  RSPS    R R RS+SRSRSRS SRSRSYS R
Sbjct: 190 AYIRVK------------------VDGPRSPSYG--RSRSRSRSRSRSRSNSRSRSYSPR 245

Query: 242 STSLSPRH--KHSR-RSRS 254
            +  SPR+  +HSR RSR+
Sbjct: 250 RSRGSPRYSPRHSRSRSRT 245


HSP 2 Score: 45.1 bits (105), Expect = 1.7e-03
Identity = 32/74 (43.24%), Postives = 40/74 (54.05%), Query Frame = 1

Query: 224 RSRSRSRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSR-- 283
           R    ++ RS+ G +  +  +    R       +S SRSRSRSRS SRSRS SP  SR  
Sbjct: 172 RKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSNSRSRSYSPRRSRGS 231

Query: 284 HRASRPRSRSRSRS 296
            R S   SRSRSR+
Sbjct: 232 PRYSPRHSRSRSRT 245


HSP 3 Score: 38.9 bits (89), Expect = 1.2e-01
Identity = 34/90 (37.78%), Postives = 42/90 (46.67%), Query Frame = 1

Query: 224 RSRSRSRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHR 283
           R    ++ RS+ G +  +  +    R        S  RSRSRSRSRSRSRS S       
Sbjct: 172 RKLDNTKFRSHEGETAYIRVKVDGPR------SPSYGRSRSRSRSRSRSRSNS------- 231

Query: 284 ASRPRSRSRSRSKSRSLSPNVRSDRSRSQS 314
               RSRS S  +SR  SP      SRS+S
Sbjct: 232 ----RSRSYSPRRSRG-SPRYSPRHSRSRS 243


HSP 4 Score: 33.5 bits (75), Expect = 5.2e+00
Identity = 52/228 (22.81%), Postives = 79/228 (34.65%), Query Frame = 1

Query: 96  SGSSRGGVPRRTDYRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMA-GIVDYT 155
           SG    G     D R+ V  LP     +D++D   + G +   ++   RGG     V++ 
Sbjct: 2   SGGVIRGPAGNNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFE 61

Query: 156 NYDDMKYAIRKLDDSEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYR 215
           +  D + A+   D  ++     R                   + + R  Y     RS YR
Sbjct: 62  DPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGGGRGGGGGGGVGAPRGRYGPPSRRSEYR 121

Query: 216 ----GRGRSQSRSRSRSRSRSRS--------YSGRSTSLSPRHKHSRRSRSVSEQSLSRS 275
               G   S S    +   R             G       R +    +    + +  RS
Sbjct: 122 VIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRS 181

Query: 276 RSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSLSPNVRSDRSR 311
                +  R +   P S  +  SR RSRSRSRS+S S S +    RSR
Sbjct: 182 HEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSNSRSRSYSPRRSR 229

BLAST of Cucsa.181840.4 vs. TrEMBL
Match: A0A0A0M1I3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569330 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-181
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED
Sbjct: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA
Sbjct: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV
Sbjct: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS
Sbjct: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240

Query: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300
           LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL
Sbjct: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300

Query: 301 SPNVRSDRSRSQSVDSRDQYSE 323
           SPNVRSDRSRSQSVDSRDQYSE
Sbjct: 301 SPNVRSDRSRSQSVDSRDQYSE 322

BLAST of Cucsa.181840.4 vs. TrEMBL
Match: M5VZF1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009360mg PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 6.4e-122
Identity = 235/308 (76.30%), Postives = 256/308 (83.12%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR+RE+EDLF KYGPIVDIDLK+PPRPPGYAF+EFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIRMREIEDLFMKYGPIVDIDLKVPPRPPGYAFIEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYS---RSGSSRGGVPRRTDYRVLVTGLP 120
           AIYGRDGY FDG RLRVE AHGGRGHSSS+DRYS    S SSRG   RR+DYRVLVTGLP
Sbjct: 61  AIYGRDGYNFDGYRLRVELAHGGRGHSSSMDRYSSYSHSSSSRGA-SRRSDYRVLVTGLP 120

Query: 121 SSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSR 180
            SASWQDLKDHMRRAGDVCFS+VFRD+GGM GIVDYTNYDDM+YAIRKLDDSEF+NAFSR
Sbjct: 121 PSASWQDLKDHMRRAGDVCFSQVFRDQGGMTGIVDYTNYDDMRYAIRKLDDSEFKNAFSR 180

Query: 181 AYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGR 240
           AY+RVKEYDSR SYSRSPS D RRS YSRSPSRSPY         SRS+SRSRS SY GR
Sbjct: 181 AYIRVKEYDSRRSYSRSPSYDMRRS-YSRSPSRSPY--------TSRSQSRSRSYSYGGR 240

Query: 241 STSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKS 300
             S+SP  K+   S SV   S  RS SRSR+RSR+RSRSPVSS    +  RS+SRSRS S
Sbjct: 241 IRSISPEAKYLHCSPSV---SYPRSVSRSRARSRTRSRSPVSSASPRTLSRSKSRSRSLS 295

Query: 301 RSLSPNVR 306
           RSLSP+ R
Sbjct: 301 RSLSPSAR 295

BLAST of Cucsa.181840.4 vs. TrEMBL
Match: V4T0Q8_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002007mg PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 5.6e-118
Identity = 240/310 (77.42%), Postives = 254/310 (81.94%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRT+YVGNLPGD R+REVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDAED
Sbjct: 1   MSSRSSRTLYVGNLPGDTRMREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDYRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSR--SGSSRGGVPRRTDYRVLVTGLPS 120
           AI GRDGY FDG RLRVE AHGGR HSSSVDRYS   SG SRG V RR+DYRVLVTGLPS
Sbjct: 61  AIRGRDGYNFDGYRLRVELAHGGRRHSSSVDRYSSYSSGGSRG-VSRRSDYRVLVTGLPS 120

Query: 121 SASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRA 180
           SASWQDLKDHMRRAGDVCFS+VFRDRGGM GIVDYT+YDDMKYAIRKLD SEFRNAFSR+
Sbjct: 121 SASWQDLKDHMRRAGDVCFSQVFRDRGGMTGIVDYTSYDDMKYAIRKLDRSEFRNAFSRS 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
           YVRV+EYDSR SYSRSP   SR   YSRS SRSPY         SRSRS SRS SYS RS
Sbjct: 181 YVRVREYDSRRSYSRSP---SRSPYYSRSRSRSPY--------YSRSRSPSRSWSYSPRS 240

Query: 241 TSLSPRHKHSRRS------RSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSR 300
            S SPR K+SRRS      RS S++S S S  RS SRSRSRS SP+SS  R+ R RSRSR
Sbjct: 241 RSYSPRGKYSRRSPSLSPARSASQRSPSGSPPRSFSRSRSRSSSPLSS-PRSPRGRSRSR 297

Query: 301 SRSKSRSLSP 303
           +RS SRSLSP
Sbjct: 301 TRSISRSLSP 297

BLAST of Cucsa.181840.4 vs. TrEMBL
Match: A0A067JV96_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23302 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.8e-115
Identity = 237/335 (70.75%), Postives = 255/335 (76.12%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR SRT+YVGNLPGDIR REV+DLFYKYGPIVD+DLKIPPRPPGYAFVEFED RDAED
Sbjct: 1   MSSRASRTLYVGNLPGDIRQREVKDLFYKYGPIVDVDLKIPPRPPGYAFVEFEDARDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRG--GVPRRTDYRVLVTGLPS 120
           AI GRDGY FDGCRLRVE AHGGR HSS  DRYS   SS G  G  +R+DYRVLVTGLPS
Sbjct: 61  AIRGRDGYNFDGCRLRVELAHGGRRHSSPSDRYSSYSSSSGSRGPSKRSDYRVLVTGLPS 120

Query: 121 SASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRA 180
           SASWQDLKDHMR+AGDVCFS+VFRDRGGM GIVDYTNYDDM  AIRKLDDSEFRNAFSR+
Sbjct: 121 SASWQDLKDHMRKAGDVCFSQVFRDRGGMTGIVDYTNYDDMNRAIRKLDDSEFRNAFSRS 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
           YVRV+EYDSR S             YSRSPSRSPY        RSRS SRSRSRSYS RS
Sbjct: 181 YVRVREYDSRRS-------------YSRSPSRSPY-------GRSRSWSRSRSRSYSDRS 240

Query: 241 TSLSPRHKHSRRSRSVSEQSLSRSRSRS-----RSRSRSRSRSPVSS-------RHRASR 300
            S SP  K+S RSRS S      SRSR+      SRS SRSRSP++S       R  + R
Sbjct: 241 RSKSPGAKYSHRSRSASRSRSVSSRSRTGSSPRPSRSLSRSRSPLASSPSKRSGRDLSKR 300

Query: 301 PRSRSRSRS-KSRSLSPNVRSDRSRSQSVDSRDQY 321
            RSRSRSRS  S+S S +VRSD  RSQS+DSRD+Y
Sbjct: 301 GRSRSRSRSIGSQSRSSSVRSD--RSQSMDSRDRY 313

BLAST of Cucsa.181840.4 vs. TrEMBL
Match: A0A059D2W0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B01911 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 1.7e-114
Identity = 231/326 (70.86%), Postives = 254/326 (77.91%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MS R+S TIYVGNLPGD+R+REVEDLFYK+GPIVD+DLKIPPRPPGYAFVEFED RDAED
Sbjct: 49  MSRRSSCTIYVGNLPGDVRMREVEDLFYKFGPIVDVDLKIPPRPPGYAFVEFEDPRDAED 108

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AI GRDGYKFDG RLRVE AHGGRG+SSSVDRY  SGSSRGGV R +DYRVLVTGLPSSA
Sbjct: 109 AIRGRDGYKFDGYRLRVELAHGGRGYSSSVDRYG-SGSSRGGVGRHSDYRVLVTGLPSSA 168

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMRRAGDVCFSEVFR+R G+ GIVDYT+YDDMKYAI+KLDD+ FRNAFSRAY+
Sbjct: 169 SWQDLKDHMRRAGDVCFSEVFRERDGVTGIVDYTSYDDMKYAIKKLDDTLFRNAFSRAYI 228

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RV+EYDSR SYSRSPS                     RS   SRSRS S SRSYS RS S
Sbjct: 229 RVREYDSRRSYSRSPS---------------------RSPRYSRSRSPSYSRSYSDRSRS 288

Query: 241 LSPRHKHSRRS------RSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSR 300
            SPR K+SRRS      RSVS +S S S + S SRSRSRSR+P+S   R  +  SRS  R
Sbjct: 289 RSPRKKYSRRSPSVSPARSVSSRSRSGSYAHSYSRSRSRSRTPLSPASR-GKHTSRSSRR 348

Query: 301 SKSRSLSPNVRSDRSRSQSVDSRDQY 321
           S S S SP+V   RSRS+SVDSR+ Y
Sbjct: 349 SLSESRSPSV---RSRSRSVDSREYY 348

BLAST of Cucsa.181840.4 vs. TAIR10
Match: AT1G09140.1 (AT1G09140.1 SERINE-ARGININE PROTEIN 30)

HSP 1 Score: 369.4 bits (947), Expect = 2.3e-102
Identity = 201/284 (70.77%), Postives = 220/284 (77.46%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR +RTIYVGNLPGDIR  EVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRWNRTIYVGNLPGDIRKCEVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDPRDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY FDGCRLRVE AHGGR  S SVDRYS S S+     RR+DYRVLVTGLP SA
Sbjct: 61  AIYGRDGYDFDGCRLRVEIAHGGRRFSPSVDRYSSSYSASRAPSRRSDYRVLVTGLPPSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMR+AGDVCFSEVF DR GM+G+VDY+NYDDMKYAIRKLD +EFRNAFS AY+
Sbjct: 121 SWQDLKDHMRKAGDVCFSEVFPDRKGMSGVVDYSNYDDMKYAIRKLDATEFRNAFSSAYI 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RV+EY+SR S SRSP       D S+S              RSRSRSR  S SYS +S S
Sbjct: 181 RVREYESR-SVSRSP-------DDSKS-------------YRSRSRSRGPSCSYSSKSRS 240

Query: 241 LSPRHKHSRRSRSVSE-----QSLSRSRSRSRSRSRSRSRSPVS 280
           +SP    S RSR +S       S+SRS+SRS+SRSRSRS SPVS
Sbjct: 241 VSPARSISPRSRPLSRSRSLYSSVSRSQSRSKSRSRSRSNSPVS 263


HSP 2 Score: 307.8 bits (787), Expect = 8.1e-84
Identity = 187/314 (59.55%), Postives = 207/314 (65.92%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR +RTIYVGNLPGDIR  EVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRWNRTIYVGNLPGDIRKCEVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDPRDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY FDGCRLRVE AHGGR  S SVDRYS S S+     RR+DYRVLVTGLP SA
Sbjct: 61  AIYGRDGYDFDGCRLRVEIAHGGRRFSPSVDRYSSSYSASRAPSRRSDYRVLVTGLPPSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMR+AGDVCFSEVF DR GM+G+VDY+NYDDMK                    
Sbjct: 121 SWQDLKDHMRKAGDVCFSEVFPDRKGMSGVVDYSNYDDMK-------------------Y 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDY-SRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRST 240
            +++ D+    +   S   R  +Y SRS SRSP   +     RSRSRSR  S SYS +S 
Sbjct: 181 AIRKLDATEFRNAFSSAYIRVREYESRSVSRSPDDSKSY---RSRSRSRGPSCSYSSKSR 240

Query: 241 SLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRS 300
           S+SP       +RS+S           RSR  SRSRS  SS        SRS+SRSKSRS
Sbjct: 241 SVSP-------ARSISP----------RSRPLSRSRSLYSSV-------SRSQSRSKSRS 260

Query: 301 LSPNVRSDRSRSQS 314
                   RSRS S
Sbjct: 301 --------RSRSNS 260

BLAST of Cucsa.181840.4 vs. TAIR10
Match: AT1G02840.1 (AT1G02840.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 352.8 bits (904), Expect = 2.2e-97
Identity = 197/314 (62.74%), Postives = 226/314 (71.97%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRT+YVGNLPGDIR REVEDLF KYGP+V IDLK+PPRPPGYAFVEF+D RDAED
Sbjct: 1   MSSRSSRTVYVGNLPGDIREREVEDLFSKYGPVVQIDLKVPPRPPGYAFVEFDDARDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGG-----------VPRRTDY 120
           AI+GRDGY FDG RLRVE AHGGR  SS   R S +G  RGG             RR+++
Sbjct: 61  AIHGRDGYDFDGHRLRVELAHGGR-RSSDDTRGSFNGGGRGGGRGRGDGGSRGPSRRSEF 120

Query: 121 RVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDS 180
           RVLVTGLPSSASWQDLKDHMR+ GDVCFS+V+RD  G  G+VDYT Y+DMKYA++KLDD+
Sbjct: 121 RVLVTGLPSSASWQDLKDHMRKGGDVCFSQVYRDARGTTGVVDYTCYEDMKYALKKLDDT 180

Query: 181 EFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRS 240
           EFRNAFS  YVRV+EYDSR   SRSP   SR   YS+S SRS    RGRS SRSRSRSRS
Sbjct: 181 EFRNAFSNGYVRVREYDSRKD-SRSP---SRGRSYSKSRSRS----RGRSVSRSRSRSRS 240

Query: 241 RSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRS 300
           RSRS   +S+  SP       SRS   +S SRS S  RSRSRSRS  P   +  +  P  
Sbjct: 241 RSRSPKAKSSRRSPA---KSTSRSPGPRSKSRSPSPRRSRSRSRSPLPSVQKEGSKSPSK 300

Query: 301 RSRSRSKSRSLSPN 304
            S ++S   + SP+
Sbjct: 301 PSPAKSPIHTRSPS 302


HSP 2 Score: 46.6 bits (109), Expect = 3.4e-05
Identity = 32/68 (47.06%), Postives = 36/68 (52.94%), Query Frame = 1

Query: 250 RSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSLSPNVRSDRS 309
           R R    +  SRS SR RS S+SRSRS         R  SRSRSRS+SRS SP  +S R 
Sbjct: 191 RVREYDSRKDSRSPSRGRSYSKSRSRS-------RGRSVSRSRSRSRSRSRSPKAKSSRR 250

Query: 310 RSQSVDSR 318
                 SR
Sbjct: 251 SPAKSTSR 251

BLAST of Cucsa.181840.4 vs. TAIR10
Match: AT3G49430.1 (AT3G49430.1 SER/ARG-rich protein 34A)

HSP 1 Score: 342.0 bits (876), Expect = 3.9e-94
Identity = 194/327 (59.33%), Postives = 224/327 (68.50%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MS R SR+IYVGNLPGDIR  E+ED+FYKYG IVDI+LK+PPRPP Y FVEFE  RDAED
Sbjct: 1   MSGRFSRSIYVGNLPGDIREHEIEDIFYKYGRIVDIELKVPPRPPCYCFVEFEHSRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSG-------------SSRGGVPRRT 120
           AI GRDGY  DGCRLRVE AHGGRG SSS  R    G             S+R GV R +
Sbjct: 61  AIKGRDGYNLDGCRLRVELAHGGRGQSSSDRRGGYGGGGSGYGGGGGGGGSARFGVSRHS 120

Query: 121 DYRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLD 180
           ++RV+V GLPSSASWQDLKDHMR+AGDVCF+EV RD  G  G+VDYTNYDDMKYAIRKLD
Sbjct: 121 EFRVIVRGLPSSASWQDLKDHMRKAGDVCFAEVTRDSDGTYGVVDYTNYDDMKYAIRKLD 180

Query: 181 DSEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRS 240
           D+EFRN ++R ++RVK+Y+S  S SRSPS        SRS SRS  RGRGRS SRSRS S
Sbjct: 181 DTEFRNPWARGFIRVKKYESSRSRSRSPS-----RSRSRSRSRSRSRGRGRSHSRSRSLS 240

Query: 241 RSRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRP 300
           RS+           SPR   S+  R    +SLSRS S+SRS S  + +SP        R 
Sbjct: 241 RSK-----------SPRKDLSKSPR----RSLSRSISKSRSPSPDKKKSP-------PRA 300

Query: 301 RSRSRSRSKSRSLSPNVRSDRSRSQSV 315
            SRS+SRS+SRS SP+    + R  SV
Sbjct: 301 MSRSKSRSRSRSRSPSKSPPKVREGSV 300


HSP 2 Score: 39.3 bits (90), Expect = 5.4e-03
Identity = 27/60 (45.00%), Postives = 32/60 (53.33%), Query Frame = 1

Query: 263 RSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSLSPNVRSDRSRSQSVDSRDQYSE 322
           R +    SRSRSRSP       SR RSRSRSRS+SR    +    RS S+S   R   S+
Sbjct: 194 RVKKYESSRSRSRSP-------SRSRSRSRSRSRSRGRGRSHSRSRSLSRSKSPRKDLSK 246

BLAST of Cucsa.181840.4 vs. TAIR10
Match: AT4G02430.2 (AT4G02430.2 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 330.1 bits (845), Expect = 1.5e-90
Identity = 190/294 (64.63%), Postives = 209/294 (71.09%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR REVEDLF KYGP+V IDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPRPPGYAFVEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRG------------GVPRRTD 120
           AIYGRDGY FDG  LRVE AHGGR  SS   R S SG  RG            G  RR++
Sbjct: 61  AIYGRDGYDFDGHHLRVELAHGGR-RSSHDARGSYSGRGRGGRGGGDGGGRERGPSRRSE 120

Query: 121 YRVLVTGLPSSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDD 180
           YRV+V+GLPSSASWQDLKDHMR+ G+VCFS+VFRD  G  GIVDYT+Y+DMKYA   LDD
Sbjct: 121 YRVVVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRGTTGIVDYTSYEDMKYA---LDD 180

Query: 181 SEFRNAFSRAYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSR 240
           +EFRNAFS  YVRV+EYDSR   SRSP   SR   YS+S      R RGRS SRSRSRSR
Sbjct: 181 TEFRNAFSHEYVRVREYDSRRD-SRSP---SRGRSYSKS------RSRGRSPSRSRSRSR 240

Query: 241 SRSRSYSGRSTSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRH 283
           SRS+       S SP+ K  RRS + S     RSRSRS+SRS S        RH
Sbjct: 241 SRSK-------SRSPKAKSLRRSPAKSTSRSPRSRSRSKSRSLSPRGWVTVERH 273


HSP 2 Score: 164.5 bits (415), Expect = 1.1e-40
Identity = 129/319 (40.44%), Postives = 155/319 (48.59%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR REVEDLF KYGP+V IDLKIPPRPPGYAFVEFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIREREVEDLFSKYGPVVQIDLKIPPRPPGYAFVEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGY F              GH   V+     G  R     R  Y     G     
Sbjct: 61  AIYGRDGYDF-------------DGHHLRVEL--AHGGRRSSHDARGSYSGRGRGGRGGG 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAF--SRA 180
                    R  G    SE    R  ++G+    ++ D+K  +RK  +  F   F   R 
Sbjct: 121 DGGG-----RERGPSRRSEY---RVVVSGLPSSASWQDLKDHMRKGGEVCFSQVFRDGRG 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
              + +Y S      +      R+ +S       Y       SR  SRS SR RSYS   
Sbjct: 181 TTGIVDYTSYEDMKYALDDTEFRNAFSHE-----YVRVREYDSRRDSRSPSRGRSYS--- 240

Query: 241 TSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSR 300
                                 +SRSR RS SRSRSRS   S+ R+ + +S  RS +KS 
Sbjct: 241 ----------------------KSRSRGRSPSRSRSRSRSRSKSRSPKAKSLRRSPAKST 265

Query: 301 SLSPNVRSDRSRSQSVDSR 318
           S SP  RS RS+S+S+  R
Sbjct: 301 SRSPRSRS-RSKSRSLSPR 265

BLAST of Cucsa.181840.4 vs. TAIR10
Match: AT5G52040.2 (AT5G52040.2 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 102.1 bits (253), Expect = 6.8e-22
Identity = 107/334 (32.04%), Postives = 146/334 (43.71%), Query Frame = 1

Query: 7   RTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAEDAIYGRD 66
           + ++ GN   D R  ++E LF KYG +  +D+K      G+AFV  ED RDAEDAI   D
Sbjct: 2   KPVFCGNFEYDARESDLERLFRKYGKVERVDMKA-----GFAFVYMEDERDAEDAIRALD 61

Query: 67  GYKF--DGCRLRVEFAHGGRGHSSSVDRYSRSGSSR----GGVPRRTDYRVLVTGLPSSA 126
            +++   G RLRVE+    RG +       RSG SR    G  P +T +  ++     + 
Sbjct: 62  RFEYGRTGRRLRVEWTKNDRGGA------GRSGGSRRSSSGLRPSKTLF--VINFDAQNT 121

Query: 127 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 186
             +DL+ H    G +    + R+       + Y   +D   A+   + S+  +       
Sbjct: 122 RTRDLERHFEPYGKIVNVRIRRN----FAFIQYEAQEDATRALDATNSSKLMDKVISVEY 181

Query: 187 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYR--------GRGRSQSRSRSRSRSRSR 246
            VK+ DSR +   SP     RS   R  S SPYR        GRG S    +    S   
Sbjct: 182 AVKDDDSRGN-GYSPERRRDRSPDRRRRSPSPYRRERGSPDYGRGASPVAHKRERTSPDY 241

Query: 247 SYSGRSTS------LSPRHKHS--RRSRSVS-EQSLSRSRSRSRSRSRSRSRSPVSSRHR 306
               RS S      LSP +K    RR R  S E    R+RS  + R  SRS  P   R R
Sbjct: 242 GRGRRSPSPYKRARLSPDYKRDDRRRERVASPENGAVRNRSPRKGRGESRSPPPYEKR-R 301

Query: 307 ASRPRSRSRSRSKSRSLSP-NVRSDRSRSQSVDS 317
            SR       R +SRS  P   R +RSRS+S  S
Sbjct: 302 ESRSPPPYEKRRESRSPPPYEKRRERSRSRSKSS 316

BLAST of Cucsa.181840.4 vs. NCBI nr
Match: gi|778662425|ref|XP_011659887.1| (PREDICTED: serine/arginine-rich splicing factor SR30 [Cucumis sativus])

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-180
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED
Sbjct: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA
Sbjct: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV
Sbjct: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS
Sbjct: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240

Query: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300
           LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL
Sbjct: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300

Query: 301 SPNVRSDRSRSQSVDSRDQYSE 323
           SPNVRSDRSRSQSVDSRDQYSE
Sbjct: 301 SPNVRSDRSRSQSVDSRDQYSE 322

BLAST of Cucsa.181840.4 vs. NCBI nr
Match: gi|659099466|ref|XP_008450613.1| (PREDICTED: pre-mRNA-splicing factor SF2 [Cucumis melo])

HSP 1 Score: 615.9 bits (1587), Expect = 4.0e-173
Identity = 316/324 (97.53%), Postives = 317/324 (97.84%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED
Sbjct: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120
           AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA
Sbjct: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSRSGSSRGGVPRRTDYRVLVTGLPSSA 120

Query: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180
           SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV
Sbjct: 121 SWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRAYV 180

Query: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRSTS 240
           RVKEYDSRHSYSRSPSLDSRRSDYSRSPS SPYRGRGRSQ  SRSRSRSRSRSYSGRSTS
Sbjct: 181 RVKEYDSRHSYSRSPSLDSRRSDYSRSPSGSPYRGRGRSQ--SRSRSRSRSRSYSGRSTS 240

Query: 241 LSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300
           LSPRHKHSRRSRS+SEQSL  SRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL
Sbjct: 241 LSPRHKHSRRSRSISEQSL--SRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSRSL 300

Query: 301 SPNVRSD--RSRSQSVDSRDQYSE 323
           SPNVRSD  RSRSQSVDSRDQYSE
Sbjct: 301 SPNVRSDRSRSRSQSVDSRDQYSE 320

BLAST of Cucsa.181840.4 vs. NCBI nr
Match: gi|645222096|ref|XP_008246433.1| (PREDICTED: pre-mRNA-splicing factor SF2 isoform X1 [Prunus mume])

HSP 1 Score: 445.3 bits (1144), Expect = 9.2e-122
Identity = 233/307 (75.90%), Postives = 253/307 (82.41%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR+RE+EDLF KYGPIVDIDLK+PPRPPGYAF+EFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIRMREIEDLFMKYGPIVDIDLKVPPRPPGYAFIEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSR--SGSSRGGVPRRTDYRVLVTGLPS 120
           AIYGRDGY FDG RLRVE AHGGRGHSSS+DRYS     SS  G  RR+DYRVLVTGLP 
Sbjct: 61  AIYGRDGYNFDGYRLRVELAHGGRGHSSSMDRYSSYSHSSSSHGASRRSDYRVLVTGLPP 120

Query: 121 SASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRA 180
           SASWQDLKDHMRRAGDVCFS+VFRDRGGM GIVDYTNYDDM+YAIRKLDDSEF+NAFSRA
Sbjct: 121 SASWQDLKDHMRRAGDVCFSQVFRDRGGMTGIVDYTNYDDMRYAIRKLDDSEFKNAFSRA 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
           Y+RVKEYDSR SYSRSPS D RRS YSRSPSRSPY         SRS+SRSRS SY GR 
Sbjct: 181 YIRVKEYDSRRSYSRSPSYDMRRS-YSRSPSRSPY--------MSRSQSRSRSYSYGGRI 240

Query: 241 TSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKSR 300
            S+SP  K+  RS S    S  RS SRSR+RSR+RSRSPVSS    +  RS+SRSRS S 
Sbjct: 241 RSISPEAKYLHRSPSA---SYPRSVSRSRARSRTRSRSPVSSARPRTLSRSKSRSRSLSW 295

Query: 301 SLSPNVR 306
           SLSP+ R
Sbjct: 301 SLSPSAR 295

BLAST of Cucsa.181840.4 vs. NCBI nr
Match: gi|595826943|ref|XP_007205653.1| (hypothetical protein PRUPE_ppa009360mg [Prunus persica])

HSP 1 Score: 445.3 bits (1144), Expect = 9.2e-122
Identity = 235/308 (76.30%), Postives = 256/308 (83.12%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRTIYVGNLPGDIR+RE+EDLF KYGPIVDIDLK+PPRPPGYAF+EFED RDA+D
Sbjct: 1   MSSRSSRTIYVGNLPGDIRMREIEDLFMKYGPIVDIDLKVPPRPPGYAFIEFEDARDADD 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYS---RSGSSRGGVPRRTDYRVLVTGLP 120
           AIYGRDGY FDG RLRVE AHGGRGHSSS+DRYS    S SSRG   RR+DYRVLVTGLP
Sbjct: 61  AIYGRDGYNFDGYRLRVELAHGGRGHSSSMDRYSSYSHSSSSRGA-SRRSDYRVLVTGLP 120

Query: 121 SSASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSR 180
            SASWQDLKDHMRRAGDVCFS+VFRD+GGM GIVDYTNYDDM+YAIRKLDDSEF+NAFSR
Sbjct: 121 PSASWQDLKDHMRRAGDVCFSQVFRDQGGMTGIVDYTNYDDMRYAIRKLDDSEFKNAFSR 180

Query: 181 AYVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGR 240
           AY+RVKEYDSR SYSRSPS D RRS YSRSPSRSPY         SRS+SRSRS SY GR
Sbjct: 181 AYIRVKEYDSRRSYSRSPSYDMRRS-YSRSPSRSPY--------TSRSQSRSRSYSYGGR 240

Query: 241 STSLSPRHKHSRRSRSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSRSRSKS 300
             S+SP  K+   S SV   S  RS SRSR+RSR+RSRSPVSS    +  RS+SRSRS S
Sbjct: 241 IRSISPEAKYLHCSPSV---SYPRSVSRSRARSRTRSRSPVSSASPRTLSRSKSRSRSLS 295

Query: 301 RSLSPNVR 306
           RSLSP+ R
Sbjct: 301 RSLSPSAR 295

BLAST of Cucsa.181840.4 vs. NCBI nr
Match: gi|567881737|ref|XP_006433427.1| (hypothetical protein CICLE_v10002007mg [Citrus clementina])

HSP 1 Score: 432.2 bits (1110), Expect = 8.1e-118
Identity = 240/310 (77.42%), Postives = 254/310 (81.94%), Query Frame = 1

Query: 1   MSSRNSRTIYVGNLPGDIRLREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDVRDAED 60
           MSSR+SRT+YVGNLPGD R+REVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFED RDAED
Sbjct: 1   MSSRSSRTLYVGNLPGDTRMREVEDLFYKYGPIVDIDLKIPPRPPGYAFVEFEDYRDAED 60

Query: 61  AIYGRDGYKFDGCRLRVEFAHGGRGHSSSVDRYSR--SGSSRGGVPRRTDYRVLVTGLPS 120
           AI GRDGY FDG RLRVE AHGGR HSSSVDRYS   SG SRG V RR+DYRVLVTGLPS
Sbjct: 61  AIRGRDGYNFDGYRLRVELAHGGRRHSSSVDRYSSYSSGGSRG-VSRRSDYRVLVTGLPS 120

Query: 121 SASWQDLKDHMRRAGDVCFSEVFRDRGGMAGIVDYTNYDDMKYAIRKLDDSEFRNAFSRA 180
           SASWQDLKDHMRRAGDVCFS+VFRDRGGM GIVDYT+YDDMKYAIRKLD SEFRNAFSR+
Sbjct: 121 SASWQDLKDHMRRAGDVCFSQVFRDRGGMTGIVDYTSYDDMKYAIRKLDRSEFRNAFSRS 180

Query: 181 YVRVKEYDSRHSYSRSPSLDSRRSDYSRSPSRSPYRGRGRSQSRSRSRSRSRSRSYSGRS 240
           YVRV+EYDSR SYSRSP   SR   YSRS SRSPY         SRSRS SRS SYS RS
Sbjct: 181 YVRVREYDSRRSYSRSP---SRSPYYSRSRSRSPY--------YSRSRSPSRSWSYSPRS 240

Query: 241 TSLSPRHKHSRRS------RSVSEQSLSRSRSRSRSRSRSRSRSPVSSRHRASRPRSRSR 300
            S SPR K+SRRS      RS S++S S S  RS SRSRSRS SP+SS  R+ R RSRSR
Sbjct: 241 RSYSPRGKYSRRSPSLSPARSASQRSPSGSPPRSFSRSRSRSSSPLSS-PRSPRGRSRSR 297

Query: 301 SRSKSRSLSP 303
           +RS SRSLSP
Sbjct: 301 TRSISRSLSP 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SR30_ARATH4.0e-10170.77Serine/arginine-rich splicing factor SR30 OS=Arabidopsis thaliana GN=SR30 PE=1 S... [more]
SR34_ARATH3.9e-9662.74Serine/arginine-rich-splicing factor SR34 OS=Arabidopsis thaliana GN=SR34 PE=1 S... [more]
SR34A_ARATH6.9e-9359.33Serine/arginine-rich splicing factor SR34A OS=Arabidopsis thaliana GN=SR34A PE=2... [more]
SR34B_ARATH2.7e-8964.63Serine/arginine-rich splicing factor SR34B OS=Arabidopsis thaliana GN=SR34B PE=2... [more]
SRS1B_DANRE4.1e-6154.05Serine/arginine-rich splicing factor 1B OS=Danio rerio GN=srsf1b PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M1I3_CUCSA8.0e-181100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G569330 PE=4 SV=1[more]
M5VZF1_PRUPE6.4e-12276.30Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009360mg PE=4 SV=1[more]
V4T0Q8_9ROSI5.6e-11877.42Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002007mg PE=4 SV=1[more]
A0A067JV96_JATCU5.8e-11570.75Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23302 PE=4 SV=1[more]
A0A059D2W0_EUCGR1.7e-11470.86Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B01911 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09140.12.3e-10270.77 SERINE-ARGININE PROTEIN 30[more]
AT1G02840.12.2e-9762.74 RNA-binding (RRM/RBD/RNP motifs) family protein[more]
AT3G49430.13.9e-9459.33 SER/ARG-rich protein 34A[more]
AT4G02430.21.5e-9064.63 RNA-binding (RRM/RBD/RNP motifs) family protein[more]
AT5G52040.26.8e-2232.04 RNA-binding (RRM/RBD/RNP motifs) family protein[more]
Match NameE-valueIdentityDescription
gi|778662425|ref|XP_011659887.1|1.1e-180100.00PREDICTED: serine/arginine-rich splicing factor SR30 [Cucumis sativus][more]
gi|659099466|ref|XP_008450613.1|4.0e-17397.53PREDICTED: pre-mRNA-splicing factor SF2 [Cucumis melo][more]
gi|645222096|ref|XP_008246433.1|9.2e-12275.90PREDICTED: pre-mRNA-splicing factor SF2 isoform X1 [Prunus mume][more]
gi|595826943|ref|XP_007205653.1|9.2e-12276.30hypothetical protein PRUPE_ppa009360mg [Prunus persica][more]
gi|567881737|ref|XP_006433427.1|8.1e-11877.42hypothetical protein CICLE_v10002007mg [Citrus clementina][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006376 mRNA splice site selection
biological_process GO:0048024 regulation of mRNA splicing, via spliceosome
cellular_component GO:0035061 interchromatin granule
cellular_component GO:0016607 nuclear speck
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003729 mRNA binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.181840Cucsa.181840gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.181840.4Cucsa.181840.4-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.181840.4.CDS.13Cucsa.181840.4.CDS.13CDS
Cucsa.181840.4.CDS.12Cucsa.181840.4.CDS.12CDS
Cucsa.181840.4.CDS.11Cucsa.181840.4.CDS.11CDS
Cucsa.181840.4.CDS.10Cucsa.181840.4.CDS.10CDS
Cucsa.181840.4.CDS.9Cucsa.181840.4.CDS.9CDS
Cucsa.181840.4.CDS.8Cucsa.181840.4.CDS.8CDS
Cucsa.181840.4.CDS.7Cucsa.181840.4.CDS.7CDS
Cucsa.181840.4.CDS.6Cucsa.181840.4.CDS.6CDS
Cucsa.181840.4.CDS.5Cucsa.181840.4.CDS.5CDS
Cucsa.181840.4.CDS.4Cucsa.181840.4.CDS.4CDS
Cucsa.181840.4.CDS.3Cucsa.181840.4.CDS.3CDS
Cucsa.181840.4.CDS.2Cucsa.181840.4.CDS.2CDS
Cucsa.181840.4.CDS.1Cucsa.181840.4.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.181840.4.three_prime_UTR.1Cucsa.181840.4.three_prime_UTR.1three_prime_UTR


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.181840.4.five_prime_UTR.2Cucsa.181840.4.five_prime_UTR.2five_prime_UTR
Cucsa.181840.4.five_prime_UTR.1Cucsa.181840.4.five_prime_UTR.1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 9..76
score: 6.1E-18coord: 111..173
score: 2.
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 110..181
score: 3.7E-6coord: 8..78
score: 7.5
IPR000504RNA recognition motif domainPROFILEPS50102RRMcoord: 109..187
score: 9.644coord: 7..82
score: 17
IPR012677Nucleotide-binding alpha-beta plait domainGENE3DG3DSA:3.30.70.330coord: 2..86
score: 7.1E-25coord: 105..196
score: 3.3
IPR012677Nucleotide-binding alpha-beta plait domainunknownSSF54928RNA-binding domain, RBDcoord: 5..176
score: 1.56
NoneNo IPR availablePANTHERPTHR24012FAMILY NOT NAMEDcoord: 290..299
score: 8.8E-34coord: 4..88
score: 8.8E-34coord: 106..191
score: 8.8
NoneNo IPR availablePANTHERPTHR24012:SF442SERINE/ARGININE-RICH SPLICING FACTOR 4coord: 290..299
score: 8.8E-34coord: 4..88
score: 8.8E-34coord: 106..191
score: 8.8