Cucsa.162610.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.162610.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	SPFH/Band 7/PHB domain-containing membrane-associated protein family
Location	scaffold01144 : 2181504 .. 2182456 (+)
Sequence length	723

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTTTGGAAAGGTTCAGTTGGAACTTCACCAATTTGGACTTTTGATTTACAATGCTGATGTCAAACAATTGGTGAATGTGAGTGGGCACGAATATGTCTCCTACTTGGGGCAGAAGACACAGCAAGAGGCTTCCAATCAGGCCAAAGTGGACGTTGCTGAGGCAAATGCAGAAGTGGCAGAAGTGGTGGAGGCAAATGCAGAGCTGGCGAAGAAGAAAGCAATATTGGCTAAGGGATCAGAGATGGCGGAAGTGGAAGCAGCAAAAGCAGTCTCACTGAGAGAGGCAGACCAAAaGAAAAAGGTGGAGAAGATGAATGCATTGACCATGACTGAGAAGCTTAAGGCTGAATTGTTGAGCAAAGCTAGCATTGAGTACGAAACCAAGGTGAGTTCTAAATCCTAGACAAGAACAACATGATTTGACATCAGAAGGCACAAGAGGCAAACTGTGAATTCTACTAGAAACAAATTAAGGCAGAAGCTGAGCTGTTCCAGAAACAATAAAATGCAGAGGCACAGAAGGCATTGGCTGATGCTAAATTTTATGCTTGCCAGCAAACTGCTGACAGAGACTTATATGCAAaGAAGAAAGAGGCGGAGGGACTGGTGGCACTGGCACTGGCACAGGCCTAAGCACTGTATCCCTGCTAGATGCATTGGGTGGGAACTACACCGCTCTTAGAGATTACCTAATGATCAATGGAGGGATGTTTCAAGACATTGCCAACATCAATGCCAATGCTATTAAGTATTAAGAGGCTCCAACCAATGATTAGCGTGTGGACGAATGGGAGGGATGGACAGGGTTTAGGAGGAGCTGGTAATATGGCTATGAAAGAGTTGTCTGGAGTTCATCAAATGTTGCCTCCATTGTTTCAAACAGATCATGAGCAAACTCGATTGCTTCCTCCTCCATGGATGGTAACCATGACTGACCCCAAACAGAATTGA

mRNA sequence

GTGTTTGGAAAGGTTCAGTTGGAACTTCACCAATTTGGACTTTTGATTTACAATGCTGATGTCAAACAATTGGTGAATGTGAGTGGGCACGAATATGTCTCCTACTTGGGGCAGAAGACACAGCAAGAGGCTTCCAATCAGGCCAAAGTGGACGTTGCTGAGGCAAATGCAGAAGTGGCAGAAGTGGTGGAGGCAAATGCAGAGCTGGCGAAGAAGAAAGCAATATTGGCTAAGGGATCAGAGATGGCGGAAGTGGAAGCAGCAAAAGCAGTCTCACTGAGAGAGGCAGACCAAAAGAAAAAGGTGGAGAAGATGAATGCATTGACCATGACTGAGAAGCTTAAGGCTGAATTGTTGAGCAAAGCTAGCATTGAGTACGAAACCAAGAGACTTATATGCAAAGAAGAAAGAGGCGGAGGGACTGGTGGCACTGGCACTGGCACAGGCCTAAGCACTGTATCCCTGCTAGATGCATTGGGTGGGAACTACACCGCTCTTAGAGATTACCTAATGATCAATGGAGGGATGTTTCAAGACATTGCCAACATCAATGCCAATGCTATTAAGGATGGACAGGGTTTAGGAGGAGCTGGTAATATGGCTATGAAAGAGTTGTCTGGAGTTCATCAAATGTTGCCTCCATTGTTTCAAACAGATCATGAGCAAACTCGATTGCTTCCTCCTCCATGGATGGTAACCATGACTGACCCCAAACAGAATTGA

Coding sequence (CDS)

GTGTTTGGAAAGGTTCAGTTGGAACTTCACCAATTTGGACTTTTGATTTACAATGCTGATGTCAAACAATTGGTGAATGTGAGTGGGCACGAATATGTCTCCTACTTGGGGCAGAAGACACAGCAAGAGGCTTCCAATCAGGCCAAAGTGGACGTTGCTGAGGCAAATGCAGAAGTGGCAGAAGTGGTGGAGGCAAATGCAGAGCTGGCGAAGAAGAAAGCAATATTGGCTAAGGGATCAGAGATGGCGGAAGTGGAAGCAGCAAAAGCAGTCTCACTGAGAGAGGCAGACCAAAaGAAAAAGGTGGAGAAGATGAATGCATTGACCATGACTGAGAAGCTTAAGGCTGAATTGTTGAGCAAAGCTAGCATTGAGTACGAAACCAAGAGACTTATATGCAAaGAAGAAAGAGGCGGAGGGACTGGTGGCACTGGCACTGGCACAGGCCTAAGCACTGTATCCCTGCTAGATGCATTGGGTGGGAACTACACCGCTCTTAGAGATTACCTAATGATCAATGGAGGGATGTTTCAAGACATTGCCAACATCAATGCCAATGCTATTAAGGATGGACAGGGTTTAGGAGGAGCTGGTAATATGGCTATGAAAGAGTTGTCTGGAGTTCATCAAATGTTGCCTCCATTGTTTCAAACAGATCATGAGCAAACTCGATTGCTTCCTCCTCCATGGATGGTAACCATGACTGACCCCAAACAGAATTGA

Protein sequence

VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAIKDGQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPPWMVTMTDPKQN*

Homology

[Close]

BLAST of Cucsa.162610.1 vs. Swiss-Prot
Match: FLOT1_ARATH (Flotillin-like protein 1 OS=Arabidopsis thaliana GN=FLOT1 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 8.6e-40
Identity = 103/251 (41.04%), Postives = 146/251 (58.17%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+ T++E   + +V V E N + A+V +ANAELA KKA   K +++AEVEA KAV+LREA
Sbjct: 218 GEGTKEEIKVRTEVKVFE-NQKEADVAKANAELAMKKAAWTKDAQVAEVEATKAVALREA 277

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + + +VEKMNALT TEKLKAE LSKAS+EYETK                           
Sbjct: 278 ELQTQVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQAE 337

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G     +  G    +LLDA+  +Y+ LRD+LMIN G++Q+IA
Sbjct: 338 AQKAQADAAFYSKQKEAEGLVALASAQGTYLRTLLDAVQNDYSCLRDFLMINNGIYQEIA 397

Query: 217 NINANAIKD-----------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPPW 240
             NA A++D           G+  GG+GN AMK+++G+++MLPP+  T +EQT + PP W
Sbjct: 398 KTNAMAVRDLQPKISVWNHGGEQGGGSGN-AMKDIAGLYKMLPPVLDTVYEQTGMQPPAW 457

HSP 2 Score: 95.1 bits (235), Expect = 1.1e-18
Identity = 56/133 (42.11%), Postives = 85/133 (63.91%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL+QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQA++DV+EA     
Sbjct: 129 VFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVSEA----- 188

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S++   +  K++++    + + E  K  
Sbjct: 189 ---KMKGEIGAKERTGLTLQNAAKIDAESKIISMQRQGEGTKEEIKVRTEVKVFENQKEA 248

Query: 121 LLSKASIEYETKR 131
            ++KA+ E   K+
Sbjct: 249 DVAKANAELAMKK 253

[Close]

BLAST of Cucsa.162610.1 vs. Swiss-Prot
Match: FLOT2_ARATH (Flotillin-like protein 2 OS=Arabidopsis thaliana GN=FLOT2 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 9.5e-39
Identity = 98/246 (39.84%), Postives = 139/246 (56.50%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+ T+ E   + +V V E N + A+V +AN+ELA KKA   K +++AEVEA KAV+LREA
Sbjct: 218 GEGTKAEIKVKTEVKVFE-NQKEADVAKANSELAMKKAAWTKDAKVAEVEATKAVALREA 277

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + + +VEKMNALT TEKLKAE LSKAS+EYETK                           
Sbjct: 278 ELQTQVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQAE 337

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G     +  G    +LLDA+  +Y+ LRD+LMIN G +Q+IA
Sbjct: 338 AQKAEADATFYSKQKEAEGLVALASAQGTYLRTLLDAVQNDYSCLRDFLMINNGTYQEIA 397

Query: 217 NINANAIKD------------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPP 236
             NA A++D             QG+GGA    MK+++G+++MLPP+  T +EQT + PP 
Sbjct: 398 KTNALAVRDLQPKISVWNHGGEQGIGGASGSGMKDIAGLYKMLPPVLDTVYEQTGMQPPA 457

HSP 2 Score: 94.4 bits (233), Expect = 1.9e-18
Identity = 57/133 (42.86%), Postives = 83/133 (62.41%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQA++DVAEA     
Sbjct: 129 VFDKVQLELDQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVAEA----- 188

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S++   +  K +++    + + E  K  
Sbjct: 189 ---KMKGEIGAKERTGLTLQNAAKIDAESKIISMQRQGEGTKAEIKVKTEVKVFENQKEA 248

Query: 121 LLSKASIEYETKR 131
            ++KA+ E   K+
Sbjct: 249 DVAKANSELAMKK 253

[Close]

BLAST of Cucsa.162610.1 vs. Swiss-Prot
Match: FLOT3_ARATH (Flotillin-like protein 3 OS=Arabidopsis thaliana GN=FLOT3 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.8e-38
Identity = 101/255 (39.61%), Postives = 143/255 (56.08%), Query Frame = 1


Query: 36  LGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLRE 95
           LG+ T++E   + +V V + N + A V +A+A LA +KA L++ S +AEVEAAKAV+LRE
Sbjct: 218 LGEGTKEEIKVKTEVKVFQ-NEKEALVAKADAALAIQKAALSQNSRVAEVEAAKAVALRE 277

Query: 96  ADQKKKVEKMNALTMTEKLKAELLSKASIEYETK-------------------------- 155
           A+ + KVEKMNALT TEKLKAE LSKAS+EYETK                          
Sbjct: 278 AELQTKVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQA 337

Query: 156 ---------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDI 215
                        K++   G        G    +LL A+  +Y+A+RD+LMIN G++QDI
Sbjct: 338 EATKAAADAAFYSKQKDAEGLVAMADAQGTYLKTLLGAVNNDYSAMRDFLMINNGIYQDI 397

Query: 216 ANINANAIKD-------------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLP 241
           A  NA AI+D              QG+ G G   M +++G+++MLPP+  T +EQT + P
Sbjct: 398 AKTNAVAIRDLQPKISVWNHGGAEQGMNGGGKATMNDIAGLYKMLPPVLDTVYEQTGMQP 457

HSP 2 Score: 94.0 bits (232), Expect = 2.4e-18
Identity = 56/125 (44.80%), Postives = 81/125 (64.80%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL+QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQAK+DVAEA     
Sbjct: 130 VFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQAKIDVAEA----- 189

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S +   +  K++++    + + +  K  
Sbjct: 190 ---KMKGEVGAKERTGLTIQNAAKIDAESKIISTQRLGEGTKEEIKVKTEVKVFQNEKEA 246

Query: 121 LLSKA 123
           L++KA
Sbjct: 250 LVAKA 246

[Close]

BLAST of Cucsa.162610.1 vs. Swiss-Prot
Match: FLOT4_MEDTR (Flotillin-like protein 4 OS=Medicago truncatula GN=FLOT4 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.7e-27
Identity = 85/182 (46.70%), Postives = 104/182 (57.14%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHE-------------------------YVSY 60
           VFGKVQLEL+QFGLLIYNA+VKQLV+V GHE                             
Sbjct: 129 VFGKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARVDVSEAKMKGE 188

Query: 61  LGQK-----TQQEASN-----------------------QAKVDVAEANAEVAEVVEANA 120
           +G K     T Q A+                        + +V V E N   AEV EAN+
Sbjct: 189 IGSKLREGQTLQNAAKIDAETKIIAMQRAGEGDKEGIKVRTEVKVFE-NQREAEVAEANS 248

Query: 121 ELAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYE 130
           ELAKKKA   K +++AEVEAAKAV+LR+A+ + +VE+MNALT TEKLKAE LSKAS++YE
Sbjct: 249 ELAKKKAAWTKAAQVAEVEAAKAVALRDAELQGEVERMNALTTTEKLKAEFLSKASVQYE 308

HSP 2 Score: 119.0 bits (297), Expect = 7.1e-26
Identity = 81/191 (42.41%), Postives = 103/191 (53.93%), Query Frame = 1


Query: 61  EVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLS 120
           +V EAN EL KK+          E EA       EA+ +K +        T+  +AEL +
Sbjct: 309 KVQEANWELYKKQK---------EAEAILYEKKAEAEAQKALADATFYARTQAAEAELYA 368

Query: 121 KASIEYETKRLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDI 180
           K            KE  G  T G   G  LS  +LL+ALG NYTA+RD+LMINGGMFQ+I
Sbjct: 369 KK-----------KEAEGIVTLGNAQGVYLS--ALLNALGNNYTAVRDFLMINGGMFQEI 428

Query: 181 ANINANAIK-----------DGQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPP 240
           A INA A++            G   GG G  AMKE++GV++MLPPLF+T HEQT +LPP 
Sbjct: 429 AKINAEAVRGLEPKISIWTNGGDNSGGEG--AMKEVAGVYKMLPPLFKTVHEQTGMLPPA 475

[Close]

BLAST of Cucsa.162610.1 vs. Swiss-Prot
Match: FLOT2_MEDTR (Flotillin-like protein 2 OS=Medicago truncatula GN=FLOT2 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.4e-26
Identity = 83/181 (45.86%), Postives = 100/181 (55.25%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHE----------------------------- 60
           VF KVQLEL+QFGLLIYNA+VKQLV+V GHE                             
Sbjct: 131 VFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAKNQARVDVSEAKMKGE 190

Query: 61  YVSYL--GQKTQQEASNQAKVDVAEA---------------------NAEVAEVVEANAE 120
             S L  GQ  Q  A   A+  V                        N   AEV +AN+E
Sbjct: 191 IGSKLREGQTLQNAAKIDAETKVIAMQRAGEGEKEGIKVRTEVKVFENQREAEVAQANSE 250

Query: 121 LAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYET 130
           LAKKKA   K +++AEVEA KAV+LREA+ + +VE+MNALT TEKLKA+LLSKAS++YET
Sbjct: 251 LAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKADLLSKASVQYET 310

HSP 2 Score: 117.9 bits (294), Expect = 1.6e-25
Identity = 84/219 (38.36%), Postives = 115/219 (52.51%), Query Frame = 1


Query: 39  KTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREADQ 98
           K  Q A  +AK  VA   AE+   VE    L   + + A     A V+    V     + 
Sbjct: 260 KAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKADLLSKASVQYETKVQEANWEL 319

Query: 99  KKKVEKMNALTMTEKLKAELLSKASIE---YETKR-----LICKEERGGGTGGTGTGTGL 158
            KK ++  A+   +K +AE   KAS +   Y +K+     L  K++   G    G   G 
Sbjct: 320 YKKQKETEAILYEKKAEAEA-QKASADATFYASKQAAEAELYAKKKEAEGIVTLGQAQGA 379

Query: 159 STVSLLDALGGNYTALRDYLMINGGMFQDIANINANAIK-----------DGQGLGGA-- 218
              +LL+ALG +YTA+RDYLMING MFQ+IA INA AI+            G   GG   
Sbjct: 380 YVSTLLNALGNDYTAVRDYLMINGDMFQEIAKINAEAIRGLEPKISIWTNGGDNSGGITD 439

Query: 219 GNMAMKELSGVHQMLPPLFQTDHEQTRLLPPPWMVTMTD 237
           G M MKE++GV++MLPPLF+T HEQT +LPP WM  +++
Sbjct: 440 GAMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGALSE 477

[Close]

BLAST of Cucsa.162610.1 vs. TrEMBL
Match: A0A0A0LSK6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382450 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.4e-105
Identity = 202/209 (96.65%), Postives = 203/209 (97.13%), Query Frame = 1


Query: 29  GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA 88
           GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA
Sbjct: 41  GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA 100

Query: 89  KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT 148
           KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT
Sbjct: 101 KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT 160

Query: 149 GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAI------KDGQGLGGAGNMAM 208
           GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAI      +DGQGLGGAGNMAM
Sbjct: 161 GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAINVWTNGRDGQGLGGAGNMAM 220

Query: 209 KELSGVHQMLPPLFQTDHEQTRLLPPPWM 232
           KELSGVHQMLPPLFQTDHEQTRLLPPPWM
Sbjct: 221 KELSGVHQMLPPLFQTDHEQTRLLPPPWM 249

[Close]

BLAST of Cucsa.162610.1 vs. TrEMBL
Match: I1I4S7_BRADI (Uncharacterized protein OS=Brachypodium distachyon PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.0e-52
Identity = 137/317 (43.22%), Postives = 172/317 (54.26%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANA--- 60
           VF  VQLEL QFGL+IYNA+VKQLV+V GHEY SYLGQKTQQEA NQAKVDVAEA     
Sbjct: 132 VFESVQLELDQFGLIIYNANVKQLVDVPGHEYFSYLGQKTQQEAVNQAKVDVAEARMKGE 191

Query: 61  -----------EVAEVVEA--------------NAELAKKKAILAKGSEMAEVEAAKAVS 120
                      + A  V+A              N+ELA KKA   K +++A+VEAA+AV+
Sbjct: 192 VGAKEREGMTRQNAAKVDAETKVYTVKRQGEGPNSELAMKKAGWEKQAQVAQVEAAQAVA 251

Query: 121 LREADQKKKVEKMNALTMTEKLKAELLSKASIEYETK----------------------- 180
           +REA  + +VE+ NA   TEKLKAE LSKA ++YE K                       
Sbjct: 252 IREAQLQVEVERTNAARQTEKLKAEHLSKAVVDYEMKVQQANWELYNRQKAAEALLFEQE 311

Query: 181 -----------------------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTAL 235
                                   L  K++   G    G        SLL ALGG+Y AL
Sbjct: 312 KQAEARRAGADAEFFARQREAEAELYAKQKEADGLAAMGQAQSAYVASLLGALGGSYGAL 371

[Close]

BLAST of Cucsa.162610.1 vs. TrEMBL
Match: M5Y6F3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020951mg PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-48
Identity = 118/212 (55.66%), Postives = 152/212 (71.70%), Query Frame = 1


Query: 36  LGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLRE 95
           +G+    E   +A+V V E N   AEV EANAELAKKKA  AK +++AEVEAAKAV+LR+
Sbjct: 217 VGEGRMAEIKVEAEVKVYE-NVREAEVAEANAELAKKKAGWAKEAQVAEVEAAKAVALRD 276

Query: 96  ADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGTGLSTVSL 155
           A+ +K+VE+MNALT+TEKLKAE LSKAS+EYETK+   KE  G    G   G  L T  L
Sbjct: 277 AELQKEVERMNALTITEKLKAEFLSKASVEYETKK---KEAEGLMALGQAQGAYLRT--L 336

Query: 156 LDALGGNYTALRDYLMINGGMFQDIANINANAIK----------DGQGLGG-AGNMAMKE 215
           LDA+GGNY A+RD++MI  GMFQ+IA INA+A++          +G G GG  G+ AMKE
Sbjct: 337 LDAVGGNYAAMRDFMMIKSGMFQEIAKINADAVRGLQPKISIWTNGGGEGGDGGHGAMKE 396

Query: 216 LSGVHQMLPPLFQTDHEQTRLLPPPWMVTMTD 237
           ++ V++MLPPLF+T HEQT +LPP WM T++D
Sbjct: 397 VAEVYKMLPPLFKTVHEQTGMLPPAWMGTLSD 422

[Close]

BLAST of Cucsa.162610.1 vs. TrEMBL
Match: M5Y6F3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020951mg PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-17
Identity = 48/55 (87.27%), Postives = 52/55 (94.55%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEA 56
           VFGKVQLEL+QFGLLIYNA+VKQLV+V GHEY SYLGQKTQ EA+NQAKVDVAEA
Sbjct: 129 VFGKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQAKVDVAEA 183

HSP 2 Score: 197.6 bits (501), Expect = 1.7e-47
Identity = 130/283 (45.94%), Postives = 167/283 (59.01%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQL----------VNVSGHEYVSYLGQKT---------- 60
           VFGKVQLEL+QFGLLIYNA+VKQL          V+VS  +    +G K           
Sbjct: 129 VFGKVQLELNQFGLLIYNANVKQLTQVEAANQAKVDVSEAKMKGEIGAKLSKAQTLQNAA 188

Query: 61  ------------------QQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEM 120
                             ++E   + +V V E N   AEV EANAELAKKKA   K +E+
Sbjct: 189 KIDAETKIIATQRQGEGKKEEIRVRTQVMVYE-NEREAEVAEANAELAKKKAGWTKEAEV 248

Query: 121 AEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTG 180
           A+VE AK+V+LREA  + +VE+MN LT+TEKLKAE LS+A+++YETK+   +E       
Sbjct: 249 AQVEGAKSVALREAQLQWEVERMNGLTLTEKLKAEFLSQATVDYETKK---RETEVLVAL 308

Query: 181 GTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAIK------------- 232
               GT L T  LL+ALGG+Y ALRDY+MIN G+FQ++A +NA AIK             
Sbjct: 309 AQAQGTYLRT--LLEALGGDYAALRDYMMINNGVFQEMAKVNAQAIKGLQPKISIWTSGE 368

[Close]

BLAST of Cucsa.162610.1 vs. TrEMBL
Match: A0A087GD40_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G430000 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.9e-42
Identity = 127/333 (38.14%), Postives = 168/333 (50.45%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHE-------------------------YVSY 60
           VF KVQLEL QFGL+IYNA+VKQLV+V GHE                             
Sbjct: 129 VFDKVQLELDQFGLIIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVSEAKMKGE 188

Query: 61  LGQK--TQQEASNQAKVDV---------------AEANAEV----------AEVVEANAE 120
           +G K  T     N AK+D                AE N +           A+V +ANAE
Sbjct: 189 IGAKERTGLTLQNAAKIDAESKIITMQRQGEGTKAEINVKTEIKVFENQKEADVAKANAE 248

Query: 121 LAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYET 180
           LA KKA   K +++AEVEA KAV+LR A  + +VEKMNALT TEKLKAE LSKAS+EYET
Sbjct: 249 LAMKKAAWTKDAQVAEVEATKAVALRTAQLQTEVEKMNALTRTEKLKAEFLSKASVEYET 308

Query: 181 K-----------------------------------RLICKEERGGGTGGTGTGTGLSTV 235
           K                                       K++   G     +  G    
Sbjct: 309 KVQEANWELYNKQKQAEAVLYEKQKEAEAQRAAADAAFYSKQKEAEGLVALASAQGTYLR 368

[Close]

BLAST of Cucsa.162610.1 vs. TAIR10
Match: AT5G25250.1 (AT5G25250.1 SPFH/Band 7/PHB domain-containing membrane-associated protein family)

HSP 1 Score: 165.2 bits (417), Expect = 4.9e-41
Identity = 103/251 (41.04%), Postives = 146/251 (58.17%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+ T++E   + +V V E N + A+V +ANAELA KKA   K +++AEVEA KAV+LREA
Sbjct: 218 GEGTKEEIKVRTEVKVFE-NQKEADVAKANAELAMKKAAWTKDAQVAEVEATKAVALREA 277

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + + +VEKMNALT TEKLKAE LSKAS+EYETK                           
Sbjct: 278 ELQTQVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQAE 337

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G     +  G    +LLDA+  +Y+ LRD+LMIN G++Q+IA
Sbjct: 338 AQKAQADAAFYSKQKEAEGLVALASAQGTYLRTLLDAVQNDYSCLRDFLMINNGIYQEIA 397

Query: 217 NINANAIKD-----------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPPW 240
             NA A++D           G+  GG+GN AMK+++G+++MLPP+  T +EQT + PP W
Sbjct: 398 KTNAMAVRDLQPKISVWNHGGEQGGGSGN-AMKDIAGLYKMLPPVLDTVYEQTGMQPPAW 457

HSP 2 Score: 95.1 bits (235), Expect = 6.2e-20
Identity = 56/133 (42.11%), Postives = 85/133 (63.91%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL+QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQA++DV+EA     
Sbjct: 129 VFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVSEA----- 188

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S++   +  K++++    + + E  K  
Sbjct: 189 ---KMKGEIGAKERTGLTLQNAAKIDAESKIISMQRQGEGTKEEIKVRTEVKVFENQKEA 248

Query: 121 LLSKASIEYETKR 131
            ++KA+ E   K+
Sbjct: 249 DVAKANAELAMKK 253

[Close]

BLAST of Cucsa.162610.1 vs. TAIR10
Match: AT5G25260.1 (AT5G25260.1 SPFH/Band 7/PHB domain-containing membrane-associated protein family)

HSP 1 Score: 161.8 bits (408), Expect = 5.4e-40
Identity = 98/246 (39.84%), Postives = 139/246 (56.50%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+ T+ E   + +V V E N + A+V +AN+ELA KKA   K +++AEVEA KAV+LREA
Sbjct: 218 GEGTKAEIKVKTEVKVFE-NQKEADVAKANSELAMKKAAWTKDAKVAEVEATKAVALREA 277

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + + +VEKMNALT TEKLKAE LSKAS+EYETK                           
Sbjct: 278 ELQTQVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQAE 337

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G     +  G    +LLDA+  +Y+ LRD+LMIN G +Q+IA
Sbjct: 338 AQKAEADATFYSKQKEAEGLVALASAQGTYLRTLLDAVQNDYSCLRDFLMINNGTYQEIA 397

Query: 217 NINANAIKD------------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPP 236
             NA A++D             QG+GGA    MK+++G+++MLPP+  T +EQT + PP 
Sbjct: 398 KTNALAVRDLQPKISVWNHGGEQGIGGASGSGMKDIAGLYKMLPPVLDTVYEQTGMQPPA 457

HSP 2 Score: 94.4 bits (233), Expect = 1.1e-19
Identity = 57/133 (42.86%), Postives = 83/133 (62.41%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQA++DVAEA     
Sbjct: 129 VFDKVQLELDQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARIDVAEA----- 188

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S++   +  K +++    + + E  K  
Sbjct: 189 ---KMKGEIGAKERTGLTLQNAAKIDAESKIISMQRQGEGTKAEIKVKTEVKVFENQKEA 248

Query: 121 LLSKASIEYETKR 131
            ++KA+ E   K+
Sbjct: 249 DVAKANSELAMKK 253

[Close]

BLAST of Cucsa.162610.1 vs. TAIR10
Match: AT5G64870.1 (AT5G64870.1 SPFH/Band 7/PHB domain-containing membrane-associated protein family)

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-39
Identity = 101/255 (39.61%), Postives = 143/255 (56.08%), Query Frame = 1


Query: 36  LGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLRE 95
           LG+ T++E   + +V V + N + A V +A+A LA +KA L++ S +AEVEAAKAV+LRE
Sbjct: 218 LGEGTKEEIKVKTEVKVFQ-NEKEALVAKADAALAIQKAALSQNSRVAEVEAAKAVALRE 277

Query: 96  ADQKKKVEKMNALTMTEKLKAELLSKASIEYETK-------------------------- 155
           A+ + KVEKMNALT TEKLKAE LSKAS+EYETK                          
Sbjct: 278 AELQTKVEKMNALTRTEKLKAEFLSKASVEYETKVQEANWELYNKQKQAEAVLYEKQKQA 337

Query: 156 ---------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDI 215
                        K++   G        G    +LL A+  +Y+A+RD+LMIN G++QDI
Sbjct: 338 EATKAAADAAFYSKQKDAEGLVAMADAQGTYLKTLLGAVNNDYSAMRDFLMINNGIYQDI 397

Query: 216 ANINANAIKD-------------GQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLP 241
           A  NA AI+D              QG+ G G   M +++G+++MLPP+  T +EQT + P
Sbjct: 398 AKTNAVAIRDLQPKISVWNHGGAEQGMNGGGKATMNDIAGLYKMLPPVLDTVYEQTGMQP 457

HSP 2 Score: 94.0 bits (232), Expect = 1.4e-19
Identity = 56/125 (44.80%), Postives = 81/125 (64.80%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL+QFGL+IYNA+VKQLV+V GHEY SYLGQKTQ EA+NQAK+DVAEA     
Sbjct: 130 VFDKVQLELNQFGLVIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQAKIDVAEA----- 189

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEA-AKAVSLREADQ--KKKVEKMNALTMTEKLKAE 120
              +   E+  K+         A+++A +K +S +   +  K++++    + + +  K  
Sbjct: 190 ---KMKGEVGAKERTGLTIQNAAKIDAESKIISTQRLGEGTKEEIKVKTEVKVFQNEKEA 246

Query: 121 LLSKA 123
           L++KA
Sbjct: 250 LVAKA 246

[Close]

BLAST of Cucsa.162610.1 vs. NCBI nr
Match: gi|700207852|gb|KGN62971.1| (hypothetical protein Csa_2G382450 [Cucumis sativus])

HSP 1 Score: 389.8 bits (1000), Expect = 3.4e-105
Identity = 202/209 (96.65%), Postives = 203/209 (97.13%), Query Frame = 1


Query: 29  GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA 88
           GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA
Sbjct: 41  GHEYVSYLGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAA 100

Query: 89  KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT 148
           KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT
Sbjct: 101 KAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGT 160

Query: 149 GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAI------KDGQGLGGAGNMAM 208
           GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAI      +DGQGLGGAGNMAM
Sbjct: 161 GLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAINVWTNGRDGQGLGGAGNMAM 220

Query: 209 KELSGVHQMLPPLFQTDHEQTRLLPPPWM 232
           KELSGVHQMLPPLFQTDHEQTRLLPPPWM
Sbjct: 221 KELSGVHQMLPPLFQTDHEQTRLLPPPWM 249

[Close]

BLAST of Cucsa.162610.1 vs. NCBI nr
Match: gi|596291222|ref|XP_007226306.1| (hypothetical protein PRUPE_ppa020951mg [Prunus persica])

HSP 1 Score: 201.4 bits (511), Expect = 1.7e-48
Identity = 118/212 (55.66%), Postives = 152/212 (71.70%), Query Frame = 1


Query: 36  LGQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLRE 95
           +G+    E   +A+V V E N   AEV EANAELAKKKA  AK +++AEVEAAKAV+LR+
Sbjct: 217 VGEGRMAEIKVEAEVKVYE-NVREAEVAEANAELAKKKAGWAKEAQVAEVEAAKAVALRD 276

Query: 96  ADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTGGTGTGTGLSTVSL 155
           A+ +K+VE+MNALT+TEKLKAE LSKAS+EYETK+   KE  G    G   G  L T  L
Sbjct: 277 AELQKEVERMNALTITEKLKAEFLSKASVEYETKK---KEAEGLMALGQAQGAYLRT--L 336

Query: 156 LDALGGNYTALRDYLMINGGMFQDIANINANAIK----------DGQGLGG-AGNMAMKE 215
           LDA+GGNY A+RD++MI  GMFQ+IA INA+A++          +G G GG  G+ AMKE
Sbjct: 337 LDAVGGNYAAMRDFMMIKSGMFQEIAKINADAVRGLQPKISIWTNGGGEGGDGGHGAMKE 396

Query: 216 LSGVHQMLPPLFQTDHEQTRLLPPPWMVTMTD 237
           ++ V++MLPPLF+T HEQT +LPP WM T++D
Sbjct: 397 VAEVYKMLPPLFKTVHEQTGMLPPAWMGTLSD 422

[Close]

BLAST of Cucsa.162610.1 vs. NCBI nr
Match: gi|596291222|ref|XP_007226306.1| (hypothetical protein PRUPE_ppa020951mg [Prunus persica])

HSP 1 Score: 97.1 bits (240), Expect = 4.6e-17
Identity = 48/55 (87.27%), Postives = 52/55 (94.55%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEA 56
           VFGKVQLEL+QFGLLIYNA+VKQLV+V GHEY SYLGQKTQ EA+NQAKVDVAEA
Sbjct: 129 VFGKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQAKVDVAEA 183

HSP 2 Score: 197.6 bits (501), Expect = 2.5e-47
Identity = 130/283 (45.94%), Postives = 167/283 (59.01%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQL----------VNVSGHEYVSYLGQKT---------- 60
           VFGKVQLEL+QFGLLIYNA+VKQL          V+VS  +    +G K           
Sbjct: 129 VFGKVQLELNQFGLLIYNANVKQLTQVEAANQAKVDVSEAKMKGEIGAKLSKAQTLQNAA 188

Query: 61  ------------------QQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEM 120
                             ++E   + +V V E N   AEV EANAELAKKKA   K +E+
Sbjct: 189 KIDAETKIIATQRQGEGKKEEIRVRTQVMVYE-NEREAEVAEANAELAKKKAGWTKEAEV 248

Query: 121 AEVEAAKAVSLREADQKKKVEKMNALTMTEKLKAELLSKASIEYETKRLICKEERGGGTG 180
           A+VE AK+V+LREA  + +VE+MN LT+TEKLKAE LS+A+++YETK+   +E       
Sbjct: 249 AQVEGAKSVALREAQLQWEVERMNGLTLTEKLKAEFLSQATVDYETKK---RETEVLVAL 308

Query: 181 GTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIANINANAIK------------- 232
               GT L T  LL+ALGG+Y ALRDY+MIN G+FQ++A +NA AIK             
Sbjct: 309 AQAQGTYLRT--LLEALGGDYAALRDYMMINNGVFQEMAKVNAQAIKGLQPKISIWTSGE 368

[Close]

BLAST of Cucsa.162610.1 vs. NCBI nr
Match: gi|658004067|ref|XP_008337140.1| (PREDICTED: flotillin-like protein 4 [Malus domestica])

HSP 1 Score: 186.0 bits (471), Expect = 7.5e-44
Identity = 112/247 (45.34%), Postives = 144/247 (58.30%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+   +E   + +V V E N   AEV +ANAELAKKKA  AK + +AEVEA+KAV+LREA
Sbjct: 220 GEGKMEEIKTRTEVKVFE-NIREAEVAKANAELAKKKAGWAKEAHVAEVEASKAVALREA 279

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + +K+VE+MNALT TEKLKAELL++AS+EYETK                           
Sbjct: 280 ELQKEVERMNALTRTEKLKAELLTEASVEYETKVQSANSEFYKEQKAADAILYQREKEAE 339

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G    G   G    SL DALGGNY A+RDY+MIN GMFQ+IA
Sbjct: 340 AQKAAADAECYAKQKEAEGLMALGQAQGAYLRSLWDALGGNYAAMRDYMMINSGMFQEIA 399

Query: 217 NINANAIK------------DGQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPP 237
            INA A++             G+G  G  N AMKE++GV++ LPPLF T HEQT + PP 
Sbjct: 400 KINAEAVRGLQPKISIWTNNGGEGGDGGANGAMKEIAGVYKALPPLFNTVHEQTGMKPPA 459

[Close]

BLAST of Cucsa.162610.1 vs. NCBI nr
Match: gi|658004067|ref|XP_008337140.1| (PREDICTED: flotillin-like protein 4 [Malus domestica])

HSP 1 Score: 94.4 bits (233), Expect = 3.0e-16
Identity = 56/133 (42.11%), Postives = 82/133 (61.65%), Query Frame = 1


Query: 1   VFGKVQLELHQFGLLIYNADVKQLVNVSGHEYVSYLGQKTQQEASNQAKVDVAEANAEVA 60
           VF KVQLEL+QFGLLIYNA+VKQLV+V GHEY SYLGQK Q EA+N+A+VDVAEA     
Sbjct: 131 VFDKVQLELNQFGLLIYNANVKQLVDVRGHEYFSYLGQKIQMEAANKARVDVAEA----- 190

Query: 61  EVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREADQKKKVEKMNALT---MTEKLKAE 120
              +   E+  K+         A+++A   +   + + + K+E++   T   + E ++  
Sbjct: 191 ---KKKGEIGSKEREGLTLQNAAKIDAETKIFRTQREGEGKMEEIKTRTEVKVFENIREA 250

Query: 121 LLSKASIEYETKR 131
            ++KA+ E   K+
Sbjct: 251 EVAKANAELAKKK 255

HSP 2 Score: 184.5 bits (467), Expect = 2.2e-43
Identity = 111/247 (44.94%), Postives = 144/247 (58.30%), Query Frame = 1


Query: 37  GQKTQQEASNQAKVDVAEANAEVAEVVEANAELAKKKAILAKGSEMAEVEAAKAVSLREA 96
           G+   +E   +A+V V E N   AEV +ANAELAKKKA  AK + +AEVEA+KAV+LREA
Sbjct: 220 GEGKMEEIKTRAEVKVFE-NIREAEVAKANAELAKKKAGWAKEAHVAEVEASKAVALREA 279

Query: 97  DQKKKVEKMNALTMTEKLKAELLSKASIEYETK--------------------------- 156
           + +K+VE+MNALT TEKLKAELL++AS++YETK                           
Sbjct: 280 ELQKEVERMNALTRTEKLKAELLTEASVKYETKVQSANSEFYREQKAADAILYQREKEAE 339

Query: 157 --------RLICKEERGGGTGGTGTGTGLSTVSLLDALGGNYTALRDYLMINGGMFQDIA 216
                       K++   G    G   G    SL DALGGNY A+RDY+MIN GMFQ+IA
Sbjct: 340 AQKAAADAECYAKQKEAEGLMALGQAQGAYLRSLWDALGGNYAAMRDYMMINSGMFQEIA 399

Query: 217 NINANAIK------------DGQGLGGAGNMAMKELSGVHQMLPPLFQTDHEQTRLLPPP 237
            INA A++             G+G     N AMKE++GV++ LPPLF T HEQT + PP 
Sbjct: 400 KINAEAVRGLQPKISIWTNNGGEGGDSGANCAMKEIAGVYKALPPLFNTVHEQTGMKPPA 459

The following BLAST results are available for this feature:

BLAST of Cucsa.162610.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FLOT1_ARATH	8.6e-40	41.04	Flotillin-like protein 1 OS=Arabidopsis thaliana GN=FLOT1 PE=2 SV=1	[more]
FLOT2_ARATH	9.5e-39	39.84	Flotillin-like protein 2 OS=Arabidopsis thaliana GN=FLOT2 PE=2 SV=1	[more]
FLOT3_ARATH	2.8e-38	39.61	Flotillin-like protein 3 OS=Arabidopsis thaliana GN=FLOT3 PE=2 SV=1	[more]
FLOT4_MEDTR	1.7e-27	46.70	Flotillin-like protein 4 OS=Medicago truncatula GN=FLOT4 PE=2 SV=1	[more]
FLOT2_MEDTR	2.4e-26	45.86	Flotillin-like protein 2 OS=Medicago truncatula GN=FLOT2 PE=2 SV=1	[more]

BLAST of Cucsa.162610.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LSK6_CUCSA	2.4e-105	96.65	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382450 PE=4 SV=1	[more]
I1I4S7_BRADI	4.0e-52	43.22	Uncharacterized protein OS=Brachypodium distachyon PE=4 SV=1	[more]
M5Y6F3_PRUPE	1.2e-48	55.66	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020951mg PE=4 SV=1	[more]
M5Y6F3_PRUPE	3.2e-17	87.27	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020951mg PE=4 SV=1	[more]
A0A087GD40_ARAAL	2.9e-42	38.14	Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G430000 PE=4 SV=1	[more]

BLAST of Cucsa.162610.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G25250.1	4.9e-41	41.04	SPFH/Band 7/PHB domain-containing membrane-associated protein family	[more]
AT5G25260.1	5.4e-40	39.84	SPFH/Band 7/PHB domain-containing membrane-associated protein family	[more]
AT5G64870.1	1.6e-39	39.61	SPFH/Band 7/PHB domain-containing membrane-associated protein family	[more]

BLAST of Cucsa.162610.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207852\|gb\|KGN62971.1\|	3.4e-105	96.65	hypothetical protein Csa_2G382450 [Cucumis sativus]	[more]
gi\|596291222\|ref\|XP_007226306.1\|	1.7e-48	55.66	hypothetical protein PRUPE_ppa020951mg [Prunus persica]	[more]
gi\|596291222\|ref\|XP_007226306.1\|	4.6e-17	87.27	hypothetical protein PRUPE_ppa020951mg [Prunus persica]	[more]
gi\|658004067\|ref\|XP_008337140.1\|	7.5e-44	45.34	PREDICTED: flotillin-like protein 4 [Malus domestica]	[more]
gi\|658004067\|ref\|XP_008337140.1\|	3.0e-16	42.11	PREDICTED: flotillin-like protein 4 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR027705	Flotillin_fam

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.162610	Cucsa.162610	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.162610.1	Cucsa.162610.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.162610.1.CDS.1	Cucsa.162610.1.CDS.1	CDS
Cucsa.162610.1.CDS.2	Cucsa.162610.1.CDS.2	CDS
Cucsa.162610.1.CDS.3	Cucsa.162610.1.CDS.3	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027705	Flotillin family	PANTHER	PTHR13806	FLOTILLIN-RELATED	coord: 1..234 score: 2.6
None	No IPR available	unknown	Coil	Coil	coord: 52..72 scor
None	No IPR available	PANTHER	PTHR13806:SF23	SUBFAMILY NOT NAMED	coord: 1..234 score: 2.6