Cucsa.162540.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.162540.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	scaffold01144 : 2141395 .. 2142331 (-)
Sequence length	937

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCACAGTATAAACTTGAGTTTGAGTTACATTTTCTTGACTTGATTTTATATTTTTTCGCTTCATGTTATAATTGCTCAAATAAATTATTTGATGGACCAAAAAGAAATCCTTGCAGTTTCCCCCACAAGCATCCAAAGATTCCATTAAATGCATTTTCATCGTCTTCTACAACCCTCAATCTTCGAACTTGTTTAAATAAACCCCCCCATTATCCTTCTCCATTATTCCTCTCTATAAACTCAAATTTCATATCAATTCCTTCTTAAAATGGCTTCCAAATCTCTTTCTTTACCAACCATTCTGTTGCTCTCTCTTCTCCTTTGCTCTGCTCTGGCCACCCCATGCGACCAACCCAAAACCAAGCCAGCACCGTCCGTGCCGAAAAAGAAACCCGCCATCCCCAAAATAATCCGGCCACCGGCGGCATCTTCTCCGGCAGTACAATCGAGTTACTGTCCAAAAGACACCTTGAAGTTGGGCGTTTGTGCCGACATTCTTGGAATTGGGAGTACCGTAATTGGGAGCCCTGTTTCGAACAATTGCTGTGCTCTGCTTAGTGGGCTGACAGATGTTGAAGCTGCTGCTTGTCTTTGTACCGCCATTAAAGCCAATGTTCTTGGAATTAACTTGAATATCCCTGTTTCAATCAGTTTGCTCATCAGCTCTTGCCAAAAAACTCTTCCCGATGGCTACCAGTGTAAATGATCAAAGTTTGGTTTTTGTTTAATGGGGTTTCTTGTTTTGGTTGTTATTTAAGTTCTGTGTGGGTCAATAAGTGGGCAAGAAATTTTTTTGCCTTAATTCAGGTCAGCCTTTGTTTATCTGTCATCATTGCTAAATTACTCGCTAGCAACTAGCAAGTGTTATATATTATTTGTATGTGTGGTGAGGAGGAATAATATTATTAATTTCATCCTGTGATTTGTGTTATTTTC

mRNA sequence

TCCACAGTATAAACTTGAGTTTGAGTTACATTTTCTTGACTTGATTTTATATTTTTTCGCTTCATGTTATAATTGCTCAAATAAATTATTTGATGGACCAAAAAGAAATCCTTGCAGTTTCCCCCACAAGCATCCAAAGATTCCATTAAATGCATTTTCATCGTCTTCTACAACCCTCAATCTTCGAACTTGTTTAAATAAACCCCCCCATTATCCTTCTCCATTATTCCTCTCTATAAACTCAAATTTCATATCAATTCCTTCTTAAAATGGCTTCCAAATCTCTTTCTTTACCAACCATTCTGTTGCTCTCTCTTCTCCTTTGCTCTGCTCTGGCCACCCCATGCGACCAACCCAAAACCAAGCCAGCACCGTCCGTGCCGAAAAAGAAACCCGCCATCCCCAAAATAATCCGGCCACCGGCGGCATCTTCTCCGGCAGTACAATCGAGTTACTGTCCAAAAGACACCTTGAAGTTGGGCGTTTGTGCCGACATTCTTGGAATTGGGAGTACCGTAATTGGGAGCCCTGTTTCGAACAATTGCTGTGCTCTGCTTAGTGGGCTGACAGATGTTGAAGCTGCTGCTTGTCTTTGTACCGCCATTAAAGCCAATGTTCTTGGAATTAACTTGAATATCCCTGTTTCAATCAGTTTGCTCATCAGCTCTTGCCAAAAAACTCTTCCCGATGGCTACCAGTGTAAATGATCAAAGTTTGGTTTTTGTTTAATGGGGTTTCTTGTTTTGGTTGTTATTTAAGTTCTGTGTGGGTCAATAAGTGGGCAAGAAATTTTTTTGCCTTAATTCAGGTCAGCCTTTGTTTATCTGTCATCATTGCTAAATTACTCGCTAGCAACTAGCAAGTGTTATATATTATTTGTATGTGTGGTGAGGAGGAATAATATTATTAATTTCATCCTGTGATTTGTGTTATTTTC

Coding sequence (CDS)

ATGGCTTCCAAATCTCTTTCTTTACCAACCATTCTGTTGCTCTCTCTTCTCCTTTGCTCTGCTCTGGCCACCCCATGCGACCAACCCAAAACCAAGCCAGCACCGTCCGTGCCGAAAAAGAAACCCGCCATCCCCAAAATAATCCGGCCACCGGCGGCATCTTCTCCGGCAGTACAATCGAGTTACTGTCCAAAAGACACCTTGAAGTTGGGCGTTTGTGCCGACATTCTTGGAATTGGGAGTACCGTAATTGGGAGCCCTGTTTCGAACAATTGCTGTGCTCTGCTTAGTGGGCTGACAGATGTTGAAGCTGCTGCTTGTCTTTGTACCGCCATTAAAGCCAATGTTCTTGGAATTAACTTGAATATCCCTGTTTCAATCAGTTTGCTCATCAGCTCTTGCCAAAAAACTCTTCCCGATGGCTACCAGTGTAAATGA

Protein sequence

MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQSSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQCK*

Homology

[Close]

BLAST of Cucsa.162540.1 vs. Swiss-Prot
Match: ERLL1_ARATH (pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana GN=AZI1 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.1e-28
Identity = 73/163 (44.79%), Postives = 100/163 (61.35%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCS-ALATPCD---QPKTKPAPSVPKKKPAI----------- 60
           MASK S SL     L++L  +  +AT C+    PK KP PS PK KP             
Sbjct: 1   MASKNSASLALFFALNILFFTLTVATNCNCKPSPKPKPVPS-PKPKPVQCPPPPRPSVPS 60

Query: 61  --PKIIRPPAASSPAVQSSYCPKDTLKLGVCADILG-IGSTVIGSPVSNNCCALLSGLTD 120
             P+ + PP   +P    + CP D LKLGVCA++L  + +  +G P S  CC+L+ GL D
Sbjct: 61  PNPRPVTPPR--TPGSSGNSCPIDALKLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLVD 120

Query: 121 VEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           V+AA CLCTA++ANVLGINLN+P+S+S+L++ C + LP G+QC
Sbjct: 121 VDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKLPSGFQC 160

[Close]

BLAST of Cucsa.162540.1 vs. Swiss-Prot
Match: 14KD_DAUCA (14 kDa proline-rich protein DC2.15 OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-26
Identity = 62/145 (42.76%), Postives = 92/145 (63.45%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           M SK+ +   +     +L  AL +  ++    P P  PK KP       P    +P   +
Sbjct: 1   MGSKNSASVALFFTLNILFFALVSSTEKC---PDPYKPKPKPT------PKPTPTPYPSA 60

Query: 61  SYCPKDTLKLGVCADILG-IGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGI 120
             CP+D LKLGVCAD+L  + + VIGSP +  CC+LL GL ++EAA CLCTAIKAN+LG 
Sbjct: 61  GKCPRDALKLGVCADVLNLVHNVVIGSPPTLPCCSLLEGLVNLEAAVCLCTAIKANILGK 120

Query: 121 NLNIPVSISLLISSCQKTLPDGYQC 145
           NLN+P+++SL++++C K +P+G++C
Sbjct: 121 NLNLPIALSLVLNNCGKQVPNGFEC 136

[Close]

BLAST of Cucsa.162540.1 vs. Swiss-Prot
Match: ERLI1_ARATH (Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana GN=EARLI1 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-26
Identity = 70/168 (41.67%), Postives = 100/168 (59.52%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCS-ALATPCD---QPKTKPAPSVPKKKPAI---PKIIRPPA 60
           MASK S S+     L+++  +   AT C     PK KP PS PK KP     PK +  P+
Sbjct: 1   MASKNSASIALFFALNIIFFTLTAATDCGCNPSPKHKPVPS-PKPKPVPSPKPKPVPSPS 60

Query: 61  ASSPAV---------------QSSYCPKDTLKLGVCADILG-IGSTVIGSPVSNNCCALL 120
             SP+V                S  CP D L+LGVCA++L  + +  +G P +  CC+L+
Sbjct: 61  VPSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVLSSLLNIQLGQPSAQPCCSLI 120

Query: 121 SGLTDVEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
            GL D++AA CLCTA++ANVLGINLN+P+S+S+L++ C + +P G+QC
Sbjct: 121 QGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKVPSGFQC 167

[Close]

BLAST of Cucsa.162540.1 vs. Swiss-Prot
Match: ERLL3_ARATH (pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana GN=At4g12500 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.3e-26
Identity = 70/176 (39.77%), Postives = 100/176 (56.82%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCS-ALATPC-----DQPKTKPAPSVPKKKPAIPKIIRP--- 60
           MASK S SL     L++L  +   AT C      +P+T P+P VP  K   P I  P   
Sbjct: 1   MASKNSTSLALFFALNILFFTLTTATDCRCNLSPKPRTVPSPKVPSPKYPSPSIPSPSVP 60

Query: 61  ------PAASSPAV---------------QSSYCPKDTLKLGVCADIL-GIGSTVIGSPV 120
                 P+  +P+V                S  CP D L+LGVCA++L G+ +  +G P 
Sbjct: 61  TPSVPTPSVPTPSVPSPNPTPVTPPRTPGSSGNCPIDALRLGVCANVLSGLLNVQLGQPS 120

Query: 121 SNNCCALLSGLTDVEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           +  CC+L+ GL D++AA CLCTA++ANVLGINLN+P+S+S+L++ C + LP  +QC
Sbjct: 121 AQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRRLPSDFQC 176

[Close]

BLAST of Cucsa.162540.1 vs. Swiss-Prot
Match: ERLL2_ARATH (pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana GN=At4g12490 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.8e-25
Identity = 68/181 (37.57%), Postives = 96/181 (53.04%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCSALA------TPCDQPKTKPAPSVPKKKPAIPKIIRP--- 60
           MASK S SL     L++L  +  A       P  +P+  P P VP  K   P +  P   
Sbjct: 1   MASKNSASLALFFALNILFFTLTAGTNCRCNPSPKPRPLPNPKVPSPKVPTPSVPSPYVP 60

Query: 61  -----------PAASSPAV---------------QSSYCPKDTLKLGVCADIL-GIGSTV 120
                      P+  SP+V                S  CP D L+LGVCA++L G+ +  
Sbjct: 61  TPSVPSPSVPTPSVPSPSVPSPNPTPVIPPRTPGSSGNCPIDALRLGVCANVLSGLLNVQ 120

Query: 121 IGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQ 145
           +G P    CC+L+ GL D++AA CLCTA++ANVLGINLN+P+S+S+L++ C + LP  +Q
Sbjct: 121 LGQPSPQPCCSLIQGLVDLDAAVCLCTALRANVLGINLNVPISLSVLLNVCNRRLPSNFQ 180

[Close]

BLAST of Cucsa.162540.1 vs. TrEMBL
Match: A0A0A0LPN2_CUCSA (Proline-rich protein DC2.15 OS=Cucumis sativus GN=Csa_2G381890 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 1.2e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN
Sbjct: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQCK 146
           LNIPVSISLLISSCQKTLPDGYQCK
Sbjct: 121 LNIPVSISLLISSCQKTLPDGYQCK 145

[Close]

BLAST of Cucsa.162540.1 vs. TrEMBL
Match: A0A151TT59_CAJCA (14 kDa proline-rich protein DC2.15 OS=Cajanus cajan GN=KK1_009402 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.5e-35
Identity = 81/163 (49.69%), Postives = 101/163 (61.96%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRP---------- 60
           MAS +  + TILLLS+L  S        P  KP P  PK  P  PK   P          
Sbjct: 1   MASNTKLIATILLLSILSYSTFTGANCPPSPKPTPPSPKPTPPSPKPTPPTPKPTPPSPK 60

Query: 61  ---------PAASSPAVQSSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTD 120
                    P+ S      S CPKDTLKLG CAD+LG+ + +IG+P S+ CCAL+ GL D
Sbjct: 61  PTPPSPKPTPSPSPSPPSQSKCPKDTLKLGACADLLGLVNIIIGTPPSSKCCALIKGLAD 120

Query: 121 VEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           +EAA CLCTAIKANVLGINLN+PV++SL++S+CQKT+P G+QC
Sbjct: 121 LEAALCLCTAIKANVLGINLNVPVTLSLILSACQKTVPTGFQC 163

[Close]

BLAST of Cucsa.162540.1 vs. TrEMBL
Match: A0A061GJ47_THECC (Bimodular protein OS=Theobroma cacao GN=TCM_036975 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.0e-35
Identity = 81/145 (55.86%), Postives = 103/145 (71.03%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASK +     L+ SLLL S   + C+  K KP P+ P           PP+ + P   S
Sbjct: 1   MASKQICA-LFLVFSLLLHSTFTSACNSCKPKPTPTPPP----------PPSPACPPPPS 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           S CPKDTLKLGVCAD+LG+ + V+GSP S+ CCALLSGL D+EAA CLCTAIKA+VLGIN
Sbjct: 61  S-CPKDTLKLGVCADLLGLVNIVVGSPPSSKCCALLSGLADLEAALCLCTAIKASVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQCK 146
           LNIPVS+SL++S+CQK +P G++C+
Sbjct: 121 LNIPVSLSLILSACQKNVPAGFKCE 133

[Close]

BLAST of Cucsa.162540.1 vs. TrEMBL
Match: G7I725_MEDTR (Protease inhibitor/seed storage/LTP family protein OS=Medicago truncatula GN=MTR_1g012710 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.3e-34
Identity = 78/151 (51.66%), Postives = 102/151 (67.55%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSV-PKKKPAIPKIIRPPAASSPAVQ 60
           MAS  LS   I+L SLL  S  +  C   K  P+P   PK KP+ P     P   +P+  
Sbjct: 1   MASNKLSA-LIILFSLLAYSTFSHACGSCKPTPSPKPKPKPKPSPPPPSSTPCPPTPSTT 60

Query: 61  ------SSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIK 120
                 S  CP DTLKLGVCAD+LG+ + ++GSP S+ CCALL GL D++AA CLCTAIK
Sbjct: 61  PPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCALLQGLVDLDAAICLCTAIK 120

Query: 121 ANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           ANVLGINLN+P+++SLL+S+C+K++P G+QC
Sbjct: 121 ANVLGINLNVPITLSLLLSACEKSVPSGFQC 150

[Close]

BLAST of Cucsa.162540.1 vs. TrEMBL
Match: G7I723_MEDTR (Lipid transfer protein OS=Medicago truncatula GN=MTR_1g012690 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.1e-34
Identity = 77/154 (50.00%), Postives = 102/154 (66.23%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQ----PKTKPAPSVPKKKPAIPKIIRPPAASSP 60
           MAS  LS   I+L SLL  S  +  C      P + P PS   K    PK   PP +++P
Sbjct: 1   MASNKLSA-LIILFSLLSYSTFSHACGSCKPTPTSPPPPSTTPKASPPPKASTPPPSTTP 60

Query: 61  AVQ------SSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCT 120
                    S  CP DTLKLGVCAD+LG+ + ++GSP S+ CC L+ GL D++AA CLCT
Sbjct: 61  KSSPPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCTLIQGLADLDAAVCLCT 120

Query: 121 AIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           AIKANVLGINLN+PV++SLL+S+C+K++P+G+QC
Sbjct: 121 AIKANVLGINLNVPVTLSLLLSACEKSVPNGFQC 153

[Close]

BLAST of Cucsa.162540.1 vs. TAIR10
Match: AT2G45180.1 (AT2G45180.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 136.7 bits (343), Expect = 1.1e-32
Identity = 71/144 (49.31%), Postives = 94/144 (65.28%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASK+L++  +L+   LL     T    P T P P    K P  PK        +PAV+ 
Sbjct: 1   MASKALAVTALLITLNLLFFTFVTSTKCPPTTPKPP---KTPKSPK-------KAPAVKP 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           + CP DTLKLGVCAD+LG+ + V+GSP    CC LL GL ++EAA CLCTA+KANVLGIN
Sbjct: 61  T-CPTDTLKLGVCADLLGLVNVVVGSPPKTPCCTLLQGLANLEAAVCLCTALKANVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQC 145
           LN+P+ ++LL++ C K +P G+QC
Sbjct: 121 LNVPIDLTLLLNYCGKKVPHGFQC 133

[Close]

BLAST of Cucsa.162540.1 vs. TAIR10
Match: AT1G62510.1 (AT1G62510.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-32
Identity = 74/152 (48.68%), Postives = 98/152 (64.47%), Query Frame = 1


Query: 1   MASKSL-SLPTILLLSLLL------CSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAA 60
           MAS++  SL   L+L+ L       C     P  +PK KP+PS PK KP       P  +
Sbjct: 1   MASRTTKSLALFLILNFLFFTTISACGNCGCPSPKPKHKPSPS-PKPKPNPKPKPTPHPS 60

Query: 61  SSPAVQSSYCPKDTLKLGVCADIL-GIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAI 120
            SPA+    CP+D LKLGVCA++L G+ +  +G P    CC L+ GL D+EAAACLCTA+
Sbjct: 61  PSPAIAK--CPRDALKLGVCANVLNGLLNVTLGKPPVEPCCTLIQGLADLEAAACLCTAL 120

Query: 121 KANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           KAN+LGINLNIP+S+SLL++ C K +P G+QC
Sbjct: 121 KANILGINLNIPLSLSLLLNVCSKKVPRGFQC 149

[Close]

BLAST of Cucsa.162540.1 vs. TAIR10
Match: AT1G12090.1 (AT1G12090.1 extensin-like protein)

HSP 1 Score: 132.1 bits (331), Expect = 2.8e-31
Identity = 75/152 (49.34%), Postives = 97/152 (63.82%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALA-----TPCDQ--PKTKPAPSVPKKKPAIPKIIRPPAA 60
           MAS S++L   L L+LL  + ++     TPC    P  KP P+ PK  P           
Sbjct: 1   MASSSIAL--FLALNLLFFTTISACGSCTPCGGGCPSPKPKPT-PKPTP----------- 60

Query: 61  SSPAVQSSYCPKDTLKLGVCADIL-GIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAI 120
            SP+  SS CPKDTLKLGVCA++L G+    +G P    CC+L+ GL DVEAA CLCTA+
Sbjct: 61  -SPSSGSSKCPKDTLKLGVCANVLNGLLDLTLGKPPVEPCCSLIQGLADVEAAVCLCTAL 120

Query: 121 KANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           KAN+LGINLN+P+S+SLL++ C K LP G+QC
Sbjct: 121 KANILGINLNLPISLSLLLNVCSKQLPPGFQC 137

[Close]

BLAST of Cucsa.162540.1 vs. TAIR10
Match: AT4G12470.1 (AT4G12470.1 azelaic acid induced 1)

HSP 1 Score: 126.7 bits (317), Expect = 1.2e-29
Identity = 73/163 (44.79%), Postives = 100/163 (61.35%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCS-ALATPCD---QPKTKPAPSVPKKKPAI----------- 60
           MASK S SL     L++L  +  +AT C+    PK KP PS PK KP             
Sbjct: 1   MASKNSASLALFFALNILFFTLTVATNCNCKPSPKPKPVPS-PKPKPVQCPPPPRPSVPS 60

Query: 61  --PKIIRPPAASSPAVQSSYCPKDTLKLGVCADILG-IGSTVIGSPVSNNCCALLSGLTD 120
             P+ + PP   +P    + CP D LKLGVCA++L  + +  +G P S  CC+L+ GL D
Sbjct: 61  PNPRPVTPPR--TPGSSGNSCPIDALKLGVCANVLSSLLNIQLGQPSSQQCCSLIQGLVD 120

Query: 121 VEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           V+AA CLCTA++ANVLGINLN+P+S+S+L++ C + LP G+QC
Sbjct: 121 VDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKLPSGFQC 160

[Close]

BLAST of Cucsa.162540.1 vs. TAIR10
Match: AT4G12480.1 (AT4G12480.1 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein)

HSP 1 Score: 120.2 bits (300), Expect = 1.1e-27
Identity = 70/168 (41.67%), Postives = 100/168 (59.52%), Query Frame = 1


Query: 1   MASK-SLSLPTILLLSLLLCS-ALATPCD---QPKTKPAPSVPKKKPAI---PKIIRPPA 60
           MASK S S+     L+++  +   AT C     PK KP PS PK KP     PK +  P+
Sbjct: 1   MASKNSASIALFFALNIIFFTLTAATDCGCNPSPKHKPVPS-PKPKPVPSPKPKPVPSPS 60

Query: 61  ASSPAV---------------QSSYCPKDTLKLGVCADILG-IGSTVIGSPVSNNCCALL 120
             SP+V                S  CP D L+LGVCA++L  + +  +G P +  CC+L+
Sbjct: 61  VPSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVLSSLLNIQLGQPSAQPCCSLI 120

Query: 121 SGLTDVEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
            GL D++AA CLCTA++ANVLGINLN+P+S+S+L++ C + +P G+QC
Sbjct: 121 QGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNRKVPSGFQC 167

[Close]

BLAST of Cucsa.162540.1 vs. NCBI nr
Match: gi|449441902|ref|XP_004138721.1| (PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis sativus])

HSP 1 Score: 290.4 bits (742), Expect = 1.7e-75
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN
Sbjct: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQCK 146
           LNIPVSISLLISSCQKTLPDGYQCK
Sbjct: 121 LNIPVSISLLISSCQKTLPDGYQCK 145

[Close]

BLAST of Cucsa.162540.1 vs. NCBI nr
Match: gi|659088926|ref|XP_008445235.1| (PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-72
Identity = 140/145 (96.55%), Postives = 142/145 (97.93%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS
Sbjct: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           SYCPKDTLKLGVCADILGIGS VIGSPVSN CCALLSGLTDVEAAACLCTAIKANVLGIN
Sbjct: 61  SYCPKDTLKLGVCADILGIGSVVIGSPVSNECCALLSGLTDVEAAACLCTAIKANVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQCK 146
           L+IPVS+SLLISSCQKTLP GYQCK
Sbjct: 121 LDIPVSLSLLISSCQKTLPAGYQCK 145

[Close]

BLAST of Cucsa.162540.1 vs. NCBI nr
Match: gi|1012359007|gb|KYP70191.1| (14 kDa proline-rich protein DC2.15 [Cajanus cajan])

HSP 1 Score: 156.0 bits (393), Expect = 5.1e-35
Identity = 81/163 (49.69%), Postives = 101/163 (61.96%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRP---------- 60
           MAS +  + TILLLS+L  S        P  KP P  PK  P  PK   P          
Sbjct: 1   MASNTKLIATILLLSILSYSTFTGANCPPSPKPTPPSPKPTPPSPKPTPPTPKPTPPSPK 60

Query: 61  ---------PAASSPAVQSSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTD 120
                    P+ S      S CPKDTLKLG CAD+LG+ + +IG+P S+ CCAL+ GL D
Sbjct: 61  PTPPSPKPTPSPSPSPPSQSKCPKDTLKLGACADLLGLVNIIIGTPPSSKCCALIKGLAD 120

Query: 121 VEAAACLCTAIKANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           +EAA CLCTAIKANVLGINLN+PV++SL++S+CQKT+P G+QC
Sbjct: 121 LEAALCLCTAIKANVLGINLNVPVTLSLILSACQKTVPTGFQC 163

[Close]

BLAST of Cucsa.162540.1 vs. NCBI nr
Match: gi|590572334|ref|XP_007011824.1| (Bimodular protein [Theobroma cacao])

HSP 1 Score: 155.2 bits (391), Expect = 8.6e-35
Identity = 81/145 (55.86%), Postives = 103/145 (71.03%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSVPKKKPAIPKIIRPPAASSPAVQS 60
           MASK +     L+ SLLL S   + C+  K KP P+ P           PP+ + P   S
Sbjct: 1   MASKQICA-LFLVFSLLLHSTFTSACNSCKPKPTPTPPP----------PPSPACPPPPS 60

Query: 61  SYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIKANVLGIN 120
           S CPKDTLKLGVCAD+LG+ + V+GSP S+ CCALLSGL D+EAA CLCTAIKA+VLGIN
Sbjct: 61  S-CPKDTLKLGVCADLLGLVNIVVGSPPSSKCCALLSGLADLEAALCLCTAIKASVLGIN 120

Query: 121 LNIPVSISLLISSCQKTLPDGYQCK 146
           LNIPVS+SL++S+CQK +P G++C+
Sbjct: 121 LNIPVSLSLILSACQKNVPAGFKCE 133

[Close]

BLAST of Cucsa.162540.1 vs. NCBI nr
Match: gi|357437019|ref|XP_003588785.1| (protease inhibitor/seed storage/LTP family protein [Medicago truncatula])

HSP 1 Score: 154.1 bits (388), Expect = 1.9e-34
Identity = 78/151 (51.66%), Postives = 102/151 (67.55%), Query Frame = 1


Query: 1   MASKSLSLPTILLLSLLLCSALATPCDQPKTKPAPSV-PKKKPAIPKIIRPPAASSPAVQ 60
           MAS  LS   I+L SLL  S  +  C   K  P+P   PK KP+ P     P   +P+  
Sbjct: 1   MASNKLSA-LIILFSLLAYSTFSHACGSCKPTPSPKPKPKPKPSPPPPSSTPCPPTPSTT 60

Query: 61  ------SSYCPKDTLKLGVCADILGIGSTVIGSPVSNNCCALLSGLTDVEAAACLCTAIK 120
                 S  CP DTLKLGVCAD+LG+ + ++GSP S+ CCALL GL D++AA CLCTAIK
Sbjct: 61  PPTPSTSQKCPSDTLKLGVCADVLGLVNVIVGSPASSKCCALLQGLVDLDAAICLCTAIK 120

Query: 121 ANVLGINLNIPVSISLLISSCQKTLPDGYQC 145
           ANVLGINLN+P+++SLL+S+C+K++P G+QC
Sbjct: 121 ANVLGINLNVPITLSLLLSACEKSVPSGFQC 150

The following BLAST results are available for this feature:

BLAST of Cucsa.162540.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ERLL1_ARATH	2.1e-28	44.79	pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana GN=AZI1 PE=1 SV=1	[more]
14KD_DAUCA	1.9e-26	42.76	14 kDa proline-rich protein DC2.15 OS=Daucus carota PE=2 SV=1	[more]
ERLI1_ARATH	1.9e-26	41.67	Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana GN=EARLI1 PE=1 SV=1	[more]
ERLL3_ARATH	4.3e-26	39.77	pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana GN=At4g12500 PE=2 ...	[more]
ERLL2_ARATH	2.8e-25	37.57	pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana GN=At4g12490 PE=2 ...	[more]

BLAST of Cucsa.162540.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPN2_CUCSA	1.2e-75	100.00	Proline-rich protein DC2.15 OS=Cucumis sativus GN=Csa_2G381890 PE=4 SV=1	[more]
A0A151TT59_CAJCA	3.5e-35	49.69	14 kDa proline-rich protein DC2.15 OS=Cajanus cajan GN=KK1_009402 PE=4 SV=1	[more]
A0A061GJ47_THECC	6.0e-35	55.86	Bimodular protein OS=Theobroma cacao GN=TCM_036975 PE=4 SV=1	[more]
G7I725_MEDTR	1.3e-34	51.66	Protease inhibitor/seed storage/LTP family protein OS=Medicago truncatula GN=MTR...	[more]
G7I723_MEDTR	5.1e-34	50.00	Lipid transfer protein OS=Medicago truncatula GN=MTR_1g012690 PE=4 SV=1	[more]

BLAST of Cucsa.162540.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45180.1	1.1e-32	49.31	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]
AT1G62510.1	3.3e-32	48.68	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]
AT1G12090.1	2.8e-31	49.34	extensin-like protein	[more]
AT4G12470.1	1.2e-29	44.79	azelaic acid induced 1	[more]
AT4G12480.1	1.1e-27	41.67	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumi...	[more]

BLAST of Cucsa.162540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449441902\|ref\|XP_004138721.1\|	1.7e-75	100.00	PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis sativus]	[more]
gi\|659088926\|ref\|XP_008445235.1\|	1.8e-72	96.55	PREDICTED: pEARLI1-like lipid transfer protein 1 [Cucumis melo]	[more]
gi\|1012359007\|gb\|KYP70191.1\|	5.1e-35	49.69	14 kDa proline-rich protein DC2.15 [Cajanus cajan]	[more]
gi\|590572334\|ref\|XP_007011824.1\|	8.6e-35	55.86	Bimodular protein [Theobroma cacao]	[more]
gi\|357437019\|ref\|XP_003588785.1\|	1.9e-34	51.66	protease inhibitor/seed storage/LTP family protein [Medicago truncatula]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR016140	Bifunc_inhib/LTP/seed_store
IPR027923	Hydrophob_seed

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0008233	peptidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.162540	Cucsa.162540	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.162540.1	Cucsa.162540.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.162540.1.five_prime_UTR.1	Cucsa.162540.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.162540.1.CDS.1	Cucsa.162540.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.162540.1.three_prime_UTR.1	Cucsa.162540.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 63..144 score: 7.
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	unknown	SSF47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 66..144 score: 2.34
IPR027923	Hydrophobic seed protein	PFAM	PF14547	Hydrophob_seed	coord: 63..144 score: 6.1
None	No IPR available	GENE3D	G3DSA:1.10.110.10		coord: 62..144 score: 6.8
None	No IPR available	PANTHER	PTHR31731	FAMILY NOT NAMED	coord: 1..144 score: 1.5